WO1997021824A3 - Method for obtaining retroviral vector supernatant having high transduction efficiency - Google Patents

Method for obtaining retroviral vector supernatant having high transduction efficiency Download PDF

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Publication number
WO1997021824A3
WO1997021824A3 PCT/US1996/019904 US9619904W WO9721824A3 WO 1997021824 A3 WO1997021824 A3 WO 1997021824A3 US 9619904 W US9619904 W US 9619904W WO 9721824 A3 WO9721824 A3 WO 9721824A3
Authority
WO
WIPO (PCT)
Prior art keywords
transduction efficiency
retroviral vector
high transduction
vector supernatant
obtaining
Prior art date
Application number
PCT/US1996/019904
Other languages
French (fr)
Other versions
WO1997021824A2 (en
Inventor
Richard J Rigg
Jonathan S Dando
Sean P Forestell
Ernst Bohnlein
Original Assignee
Richard J Rigg
Jonathan S Dando
Sean P Forestell
Ernst Bohnlein
Systemix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/572,959 external-priority patent/US5910434A/en
Application filed by Richard J Rigg, Jonathan S Dando, Sean P Forestell, Ernst Bohnlein, Systemix Inc filed Critical Richard J Rigg
Priority to JP9522241A priority Critical patent/JP2000501614A/en
Priority to AU13337/97A priority patent/AU1333797A/en
Priority to EP96944812A priority patent/EP0871753A2/en
Publication of WO1997021824A2 publication Critical patent/WO1997021824A2/en
Publication of WO1997021824A3 publication Critical patent/WO1997021824A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

Abstract

This invention provides methods for obtaining retroviral supernatants having high transduction efficiency, comprising culturing producer cells in a packed-bed bioreactor.
PCT/US1996/019904 1995-12-15 1996-12-13 Method for obtaining retroviral vector supernatant having high transduction efficiency WO1997021824A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP9522241A JP2000501614A (en) 1995-12-15 1996-12-13 Method for obtaining a retroviral vector supernatant having high transduction efficiency
AU13337/97A AU1333797A (en) 1995-12-15 1996-12-13 Method for obtaining retroviral vector supernatant having high transduction efficiency
EP96944812A EP0871753A2 (en) 1995-12-15 1996-12-13 Method for obtaining retroviral vector supernatant having high transduction efficiency

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57294395A 1995-12-15 1995-12-15
US08/572,943 1995-12-15
US08/572,959 1995-12-15
US08/572,959 US5910434A (en) 1995-12-15 1995-12-15 Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant

Publications (2)

Publication Number Publication Date
WO1997021824A2 WO1997021824A2 (en) 1997-06-19
WO1997021824A3 true WO1997021824A3 (en) 1997-09-04

Family

ID=27075989

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/019904 WO1997021824A2 (en) 1995-12-15 1996-12-13 Method for obtaining retroviral vector supernatant having high transduction efficiency

Country Status (5)

Country Link
EP (1) EP0871753A2 (en)
JP (1) JP2000501614A (en)
AU (1) AU1333797A (en)
CA (1) CA2238434A1 (en)
WO (1) WO1997021824A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994134A (en) * 1998-05-04 1999-11-30 Canji, Inc. Viral production process
US6790614B1 (en) 1999-11-19 2004-09-14 Novartis Ag Selectable cell surface marker genes
EP1807510A4 (en) 2004-09-24 2008-01-23 Angioblast Systems Inc Multipotential expanded mesenchymal precursor cell progeny (memp) and uses thereof
KR20130100225A (en) 2004-09-24 2013-09-09 안지오블라스트 시스템스 인코퍼레이티드 Method of enhancing proliferation and/or survival of mesenchymal precursor cells (mpc)
KR101446634B1 (en) 2005-04-12 2014-10-16 메소블라스트, 아이엔씨. Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase
WO2008137641A2 (en) 2007-05-04 2008-11-13 Whitehead Institute For Biomedical Research Ex vivo expansion of human hematopoietic stem cells
JP6062615B2 (en) 2007-08-06 2017-01-18 メゾブラスト，インコーポレーテッド Methods for generating, repairing and / or maintaining connective tissue in vivo
DK2235197T3 (en) * 2007-12-27 2017-10-09 Baxalta GmbH Methods of cell culture
EP3121597A1 (en) 2008-08-18 2017-01-25 Mesoblast, Inc. Monoclonal antibody stro-4
WO2014022376A2 (en) 2012-08-01 2014-02-06 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
JP6901823B2 (en) 2012-08-01 2021-07-14 ユナイテッド セラピューティクス コーポレイション Treatment of pulmonary arterial hypertension with mesenchymal stem cells
EP3878452B1 (en) 2013-01-09 2023-09-20 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
ES2901383T3 (en) 2013-05-10 2022-03-22 Whitehead Inst Biomedical Res In vitro production of red blood cells with sortase-markable proteins
CN114395559A (en) * 2014-04-25 2022-04-26 吉尼松公司 Treatment of hyperbilirubinemia
PT3757206T (en) 2014-11-05 2024-05-21 Juno Therapeutics Inc Methods for transduction and cell processing
IL266175B2 (en) 2016-10-24 2024-01-01 United Therapeutics Corp Enhancement of msc immunomodulatory properties by treprostinil
KR20200062236A (en) 2017-10-16 2020-06-03 리제너론 파아마슈티컬스, 인크. Perfusion bioreactor and related methods of use
WO2024075819A1 (en) * 2022-10-07 2024-04-11 Ａｇｃ株式会社 Virus vector production method employing cell

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007150A1 (en) * 1988-02-05 1989-08-10 The Trustees Of Columbia University In The City Of Retroviral packaging cell lines and processes of using same
WO1994019478A1 (en) * 1993-02-22 1994-09-01 The Rockefeller University Production of high titer helper-free retroviruses by transient transfection
WO1996004934A1 (en) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Retroviral vectors produced by producer cell lines resistant to lysis by human serum

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007150A1 (en) * 1988-02-05 1989-08-10 The Trustees Of Columbia University In The City Of Retroviral packaging cell lines and processes of using same
WO1994019478A1 (en) * 1993-02-22 1994-09-01 The Rockefeller University Production of high titer helper-free retroviruses by transient transfection
WO1996004934A1 (en) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Retroviral vectors produced by producer cell lines resistant to lysis by human serum

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
EVANS L H ET AL: "A NEUTRALIZABLE EPITOPE COMMON TO THE ENVELOPE GLYCOPROTEINS OF ECOTROPIC, POLYTROPIC, XENOTROPIC, AND AMPHOTROPIC MURINE LEUKEMIA VIRUSES", JOURNAL OF VIROLOGY, vol. 64, no. 12, December 1990 (1990-12-01), pages 6176 - 6183, XP000653216 *
FORESTELL S P ET AL: "RETROVIRAL END-POINT TITER IS NOT PREDICTIVE OF GENE TRANSFER EFFICIENCY: IMPLICATIONS FOR VECTOR PRODUCTION", GENE THERAPY, vol. 2, no. 10, 19 December 1995 (1995-12-19), pages 723 - 730, XP000653184 *
MILLER A D: "RETROVIRUS PACKAGING CELLS", HUMAN GENE THERAPY, vol. 1, 1990, pages 5 - 14, XP000653181 *
MILLER D ET AL: "REDESIGN OF RETROVIRUS PACKAGING CELL LINES TO AVOID RECOMBINATION LEADING TO HELPER VIRUS PRODUCTION", MOLECULAR AND CELLULAR BIOLOGY, vol. 6, no. 8, August 1986 (1986-08-01), pages 2895 - 2902, XP000605368 *
RIGG R J ET AL: "A NOVEL HUMAN AMPHOTROPIC PACKAGING CELL LINE: HIGH TILTER, COMPLEMENT RESISTANCE, AND IMPROVED SAFETY", VIROLOGY, vol. 218, no. 1, 1 January 1996 (1996-01-01), pages 290 - 295, XP000570621 *
SONEOKA Y ET AL: "A TRANSIENT THREE-PLASMID EXPRESSION SYSTEM FOR THE PRODUCTION OF HIGH TITER RETROVIRAL VECTORS", NUCLEIC ACIDS RESEARCH, vol. 23, no. 4, 25 February 1995 (1995-02-25), pages 628 - 633, XP000569533 *

Also Published As

Publication number Publication date
JP2000501614A (en) 2000-02-15
EP0871753A2 (en) 1998-10-21
WO1997021824A2 (en) 1997-06-19
AU1333797A (en) 1997-07-03
CA2238434A1 (en) 1997-06-19

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