WO1997017459A1 - Process to recover polyesters from transgenic plants - Google Patents
Process to recover polyesters from transgenic plants Download PDFInfo
- Publication number
- WO1997017459A1 WO1997017459A1 PCT/US1996/017757 US9617757W WO9717459A1 WO 1997017459 A1 WO1997017459 A1 WO 1997017459A1 US 9617757 W US9617757 W US 9617757W WO 9717459 A1 WO9717459 A1 WO 9717459A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pha
- polyhydroxyalkanoate
- recover
- matter
- solubilization
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/88—Post-polymerisation treatment
- C08G63/89—Recovery of the polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
Definitions
- THIS INVENTION relates to polyester production and in particular to a microbiological process of making polyhydroxyalkanoate (PHA).
- ACCORDING TO THE INVENTION PHA is recovered from vegetable matter associated with it by solubilizing said matter. More particularly, the process comprises:
- the process may be carried out in successive steps, each using a different reagent, and with take-off of the solution from one such step before carrying out the next.
- the solubilization steps include at least one ofthe following:
- step (i) is carried out enzymatically, since chemical solubilization of
- carbohydrates such as cellulose or hemicelluloses requires vigorous conditions such as the
- carbohydrates such as starch may be carried out chemically. If desired, cellulose may be left
- Suitable enzymes are selected from carbohydrases.
- a mixture of such enzymes is used, such as those exemplified below.
- the enzymes may be used alone or in combination with living organisms such as bacteria or fungi of which
- step (ii) is carried out enzymatically using for example a hydrolase such as
- step (iii) is carried out chemically, using an oxidant, in presence of a
- nucleic acids constitute only a few percent ofthe vegetable
- Step (iv) can be carried out chemically or enzymatically or a combination thereof.
- Chemical methods include simply displacing the protein from the PHA granules by digestion
- An alternative chemical method involves oxidation using, for
- the enzyme is a protease. If desired,
- protein may be partly solubilized by protease, then more completely by oxidation.
- At least (i) and (iv) are used if the PHA is to be recovered as particles or latex.
- the vegetable matter is suitably the seeds of one or more ofthe oil-bearing plants
- rape, canola, soya, sunflower, maize, oil palm and coconut as genetically modified for
- the required genes are preferably isolated from a PHA - accumulating procaryote such as
- Alcaligenes eutrophus or Chromatium vinosum Usually such oil seeds contain oil as well as
- step (i) is carried out by a so-called biorefinery
- the PHA produced by the process is R-stereospecific and capable of a level of
- crystallinity in the range 30-70% w/w. Its molecular weight is for example over 50,000,
- BIOPOLTM PHB homopolymer particles (... ⁇ average, crystalline).
- VISCOZYMETM carbohydrase activity
- Nordisk containing arabanase, cellulase, ⁇ -glucanases, hemi-cellulase, pectinase and
- the table shows the extent to which rape seed meal was solubilized.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96942728A EP0859859A1 (en) | 1995-11-08 | 1996-11-06 | Process to recover polyesters from transgenic plants |
JP9518283A JP2000500966A (en) | 1995-11-08 | 1996-11-06 | Method for recovering polyester from transgenic plants |
CA002236894A CA2236894A1 (en) | 1995-11-08 | 1996-11-06 | Process to recover polyesters from transgenic plants |
AU11573/97A AU1157397A (en) | 1995-11-08 | 1996-11-06 | Process to recover polyesters from transgenic plants |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9522896.1 | 1995-11-08 | ||
GBGB9522896.1A GB9522896D0 (en) | 1995-11-08 | 1995-11-08 | Polyester production |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997017459A1 true WO1997017459A1 (en) | 1997-05-15 |
Family
ID=10783594
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1996/017757 WO1997017459A1 (en) | 1995-11-08 | 1996-11-06 | Process to recover polyesters from transgenic plants |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0859859A1 (en) |
JP (1) | JP2000500966A (en) |
AU (1) | AU1157397A (en) |
GB (1) | GB9522896D0 (en) |
HU (1) | HUP9902019A2 (en) |
MX (1) | MX9803700A (en) |
WO (1) | WO1997017459A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10013514A1 (en) * | 2000-03-14 | 2001-09-27 | Ufz Leipzighalle Gmbh | Enzymatic recovery of homo- or co-polymers of polyhydroxyalkanoates (PHA) from biomass comprises treating the biomass with a reducing agent that reduces the non-PHA cell components of the biomass. |
EP3205667A1 (en) | 2016-02-15 | 2017-08-16 | Brendan Patrick Purcell | Biofabricated material containing collagen fibrils |
EP3473647A1 (en) | 2017-06-29 | 2019-04-24 | Modern Meadow, Inc. | Yeast strains and methods for producing collagen |
US11214844B2 (en) | 2017-11-13 | 2022-01-04 | Modern Meadow, Inc. | Biofabricated leather articles having zonal properties |
US11352497B2 (en) | 2019-01-17 | 2022-06-07 | Modern Meadow, Inc. | Layered collagen materials and methods of making the same |
US11913166B2 (en) | 2015-09-21 | 2024-02-27 | Modern Meadow, Inc. | Fiber reinforced tissue composites |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0145233A2 (en) * | 1983-11-23 | 1985-06-19 | Imperial Chemical Industries Plc | Separation processfor a 3-hydroxybutyrate polymer |
US5110980A (en) * | 1989-04-06 | 1992-05-05 | Ecole Polytechnique | Separation of poly-β-hydroxyalkanoic acid from microbial biomass |
WO1995005472A2 (en) * | 1993-08-17 | 1995-02-23 | Michigan State University | Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants |
-
1995
- 1995-11-08 GB GBGB9522896.1A patent/GB9522896D0/en active Pending
-
1996
- 1996-11-06 AU AU11573/97A patent/AU1157397A/en not_active Abandoned
- 1996-11-06 HU HU9902019A patent/HUP9902019A2/en unknown
- 1996-11-06 WO PCT/US1996/017757 patent/WO1997017459A1/en not_active Application Discontinuation
- 1996-11-06 EP EP96942728A patent/EP0859859A1/en not_active Withdrawn
- 1996-11-06 JP JP9518283A patent/JP2000500966A/en not_active Ceased
-
1998
- 1998-05-08 MX MX9803700A patent/MX9803700A/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0145233A2 (en) * | 1983-11-23 | 1985-06-19 | Imperial Chemical Industries Plc | Separation processfor a 3-hydroxybutyrate polymer |
US5110980A (en) * | 1989-04-06 | 1992-05-05 | Ecole Polytechnique | Separation of poly-β-hydroxyalkanoic acid from microbial biomass |
WO1995005472A2 (en) * | 1993-08-17 | 1995-02-23 | Michigan State University | Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants |
Non-Patent Citations (3)
Title |
---|
POIRIER, Y. ET AL.: "PERSPECTIVES ON THE PRODUCTION OF POLYHYDROXYALKANOATES IN PLANTS", FEMS MICROBIOLOGY REVIEWS, vol. 103, 1992, pages 237 - 246, XP000651017 * |
POIRIER, Y. ET AL.: "POLYHYDROXYBUTYRATE, A BIODEGRADABLE THERMOPLASTIC, PRODUCED IN TRANSGENIC PLANTS", SCIENCE, vol. 256, 1992, pages 520 - 523, XP002026974 * |
POIRIER, Y. ET AL.: "PRODUCTION OF POLYHYDROXYALKANOATES, A FAMILY OF BIODEGRADABLE PLASTICS AND ELASTOMERS, IN BACTERIA AND PLANTS", BIO/TECHNOLOGY, vol. 13, February 1995 (1995-02-01), pages 142 - 150, XP000651028 * |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10013514A1 (en) * | 2000-03-14 | 2001-09-27 | Ufz Leipzighalle Gmbh | Enzymatic recovery of homo- or co-polymers of polyhydroxyalkanoates (PHA) from biomass comprises treating the biomass with a reducing agent that reduces the non-PHA cell components of the biomass. |
DE10013514C2 (en) * | 2000-03-14 | 2002-06-27 | Ufz Leipzighalle Gmbh | Process for the production of polyhydroxyalkanoates (PHA) or their copolymers |
US7070966B2 (en) | 2000-03-14 | 2006-07-04 | Ufz Umweltforschungszentrum Leipzig-Halle Gmbh | Method for obtaining polyhydroxyalkanoates (PHA) and the copolymers thereof |
US11913166B2 (en) | 2015-09-21 | 2024-02-27 | Modern Meadow, Inc. | Fiber reinforced tissue composites |
US10370504B2 (en) | 2016-02-15 | 2019-08-06 | Modern Meadow, Inc. | Method for making a biofabricated material containing collagen fibrils |
EP3747900A1 (en) | 2016-02-15 | 2020-12-09 | Modern Meadow, Inc. | Method for biofabricating composite material |
EP3205669A1 (en) | 2016-02-15 | 2017-08-16 | Brendan Patrick Purcell | Composite biofabricated material |
EP3205667A1 (en) | 2016-02-15 | 2017-08-16 | Brendan Patrick Purcell | Biofabricated material containing collagen fibrils |
US10301440B2 (en) | 2016-02-15 | 2019-05-28 | Modern Meadow, Inc. | Biofabricated material containing collagen fibrils |
US10370505B2 (en) | 2016-02-15 | 2019-08-06 | Modern Meadow, Inc. | Method for making biofabricated composite |
EP3205668A1 (en) | 2016-02-15 | 2017-08-16 | Brendan Patrick Purcell | Method for biofabricating composite material |
US10519285B2 (en) | 2016-02-15 | 2019-12-31 | Modern Meadow, Inc. | Method for biofabricating composite material |
EP3747901A1 (en) | 2016-02-15 | 2020-12-09 | Modern Meadow, Inc. | Biofabricated material containing collagen fibrils |
EP3205666A1 (en) | 2016-02-15 | 2017-08-16 | Brendan Patrick Purcell | Method for making a biofabricated material containing collagen fibrils |
US11001679B2 (en) | 2016-02-15 | 2021-05-11 | Modern Meadow, Inc. | Biofabricated material containing collagen fibrils |
US11542374B2 (en) | 2016-02-15 | 2023-01-03 | Modern Meadow, Inc. | Composite biofabricated material |
US11286354B2 (en) | 2016-02-15 | 2022-03-29 | Modern Meadow, Inc. | Method for making a biofabricated material containing collagen fibrils |
US11530304B2 (en) | 2016-02-15 | 2022-12-20 | Modern Meadow, Inc. | Biofabricated material containing collagen fibrils |
US11525042B2 (en) | 2016-02-15 | 2022-12-13 | Modern Meadow, Inc. | Composite biofabricated material |
EP3473647A1 (en) | 2017-06-29 | 2019-04-24 | Modern Meadow, Inc. | Yeast strains and methods for producing collagen |
US11214844B2 (en) | 2017-11-13 | 2022-01-04 | Modern Meadow, Inc. | Biofabricated leather articles having zonal properties |
US11352497B2 (en) | 2019-01-17 | 2022-06-07 | Modern Meadow, Inc. | Layered collagen materials and methods of making the same |
Also Published As
Publication number | Publication date |
---|---|
HUP9902019A2 (en) | 1999-10-28 |
GB9522896D0 (en) | 1996-01-10 |
EP0859859A1 (en) | 1998-08-26 |
AU1157397A (en) | 1997-05-29 |
MX9803700A (en) | 1998-09-30 |
JP2000500966A (en) | 2000-02-02 |
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