WO1997016430A1 - Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases - Google Patents
Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases Download PDFInfo
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- WO1997016430A1 WO1997016430A1 PCT/US1996/017447 US9617447W WO9716430A1 WO 1997016430 A1 WO1997016430 A1 WO 1997016430A1 US 9617447 W US9617447 W US 9617447W WO 9716430 A1 WO9716430 A1 WO 9716430A1
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- 0 *C1C(N*)NC(*)C(*)N2C1CCCC2 Chemical compound *C1C(N*)NC(*)C(*)N2C1CCCC2 0.000 description 7
- VXILVSMPTBRDEC-UHFFFAOYSA-N N=C1NCCSCC1 Chemical compound N=C1NCCSCC1 VXILVSMPTBRDEC-UHFFFAOYSA-N 0.000 description 1
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Definitions
- This application is directed to inhibitors of nitric oxide synthase, and in particular cyclic amidines.
- nitric oxide a reactive, inorganic radical gas as a molecule contributing to important physiological and pathological processes is one of the major biological revelations of recent times.
- This molecule is produced under a variety of physiological and pathological conditions by cells mediating vital biological functions. Examples include endothelial cells lining the blood vessels; nitric oxide derived from these cells relaxes smooth muscle and regulates blood pressure and has significant effects on the function of circulating blood cells such as platelets and neutrophils as well as on smooth muscle, both of the blood vessels and also of other organs such as the airways.
- nitric oxide serves as a neurotransmitter in non- adrenergic non-cholinergic neurons.
- nitric oxide appears to be produced in small amounts on an intermittent basis in response to various endogenous molecular signals.
- nitric oxide can be synthesized in much larger amounts on a protracted basis. Its production is induced by exogenous or endogenous inflammatory stimuli, notably endotoxin and cytokines elaborated by cells of the host defense system in response to infectious and inflammatory stimuli. This induced production results in prolonged nitric oxide release which contributes both to host defense processes such as the killing of bacteria and viruses as well as pathology associated with acute and chronic inflammation in a wide variety of diseases.
- nitric oxide synthases which utilize the amino acid arginine and molecular oxygen as co-substrates has provided an understanding of the biochemistry of this molecule and provides distinct pharmacological targets for the inhibition of the synthesis of this mediator, which should provide significant beneficial effects in a wide variety of diseases.
- Nitric oxide and L-citrulline are formed from L-arginine via the dioxygenase activity of specific nitric oxide synthases (NOSs) in mammalian cells.
- NOSs specific nitric oxide synthases
- L-arginine, ⁇ 2 and NADPH are cosubstrates while FMN, FAD and tetrahydrobiopterin are cofactors.
- NOSs fall into two distinct classes, constitutive NOS (cNOS) and inducible NOS (iNOS) . Two constitutive NOSs have been identified. They are:
- a constitutive, Ca ++ /calmodulin dependent enzyme located in the endothelium and elsewhere (ecNOS or NOS 3), that releases NO in response to receptor or physical stimulation,
- the third isoform identified is inducible NOS (iNOS or NOS 2): (iii) a Ca ++ independent enzyme which is induced after activation of vascular smooth muscle, macrophages, endothelial cells, and a large number of other cells by endotoxin and cytokines. Once expressed, this inducible NO synthase produces NO in relatively large amounts for long periods of time.
- Spectral studies of both the mouse macrophage iNOS and rat brain ncNOS have shown that these enzymes (which have been classified as P-450-like enzymes from their CO-difference spectra) contain a heme moiety.
- the structural similarity between NOS and the P-450/flavoprotein complex suggests that the NOS reaction mechanism may be similar to P-450 hydroxylation and/or peroxidation.
- NOS belongs to a class of flavohemeproteins which contain both heme and flavin binding regions within a single protein in contrast to the multiprotein NADPH oxidase or Cytochrome P-450/NADPH Cyt c reductase complexes.
- NOS 3 acts as an autocoid mediating a number of physiological responses.
- Two distinct cDNAs accounting for the activity of NOS 1 and NOS 3 in man have been cloned, one for NOS 1 (Nakane et. al, FEBS ⁇ Letters, 316, 175-182, 1993) which is present in the brain and a number of peripheral tissues, the other for an enzyme present in endothelium
- NOS 3 Marsden et. al, FEBS tters, 307, 287-293, 1992. This latter enzyme is critical for production of NO to maintain vasorelaxation.
- a second class of enzyme, iNOS or NOS 2 has been cloned from human liver (Geller et. al, PNAS, 90, 3491-5, 1993), and identified in more than a dozen other cells and tissues, including smooth muscle cells, chondrocytes, the kidney and airways.
- this enzyme is induced upon exposure to cytokines such as gamma interferon (IFN- ⁇ ), interleukin- l ⁇ (IL-l ⁇ ), tumor necrosis factor (TNF- ⁇ ) and LPS (lipopolysaccharide).
- cytokines such as gamma interferon (IFN- ⁇ ), interleukin- l ⁇ (IL-l ⁇ ), tumor necrosis factor (TNF- ⁇ ) and LPS (lipopolysaccharide).
- IFN- ⁇ gamma interferon
- IL-l ⁇ interleukin- l ⁇
- TNF- ⁇ tumor necrosis factor
- LPS lipopolysaccharide
- Endothelium derived relaxation factor has been shown to be produced by NOS 3 (Moncada et. al, Pharmacol. Reviews, 43, 109-142, 1991). Studies with substrate analog inhibitors of NOS have shown a role for NO in regulating blood pressure in animals and blood flow in man, a function attributed to NOS 3. A transgenic mouse deficient in functional NOS 3 was shown to be hypertensive, thus validating the role of NO synthesis by NOS 3 in the regulation of blood pressure (Huang et al, Nature, 377, 239-242, 1995).
- NO has also been shown to be an effector of the cytotoxic effects of activated macrophages (Nathan, FASEB J., 6, 3051-64, 1992) for fighting tumour cells and invading microorganisms (Wright et al, Card. Res., 26 ,48-57, 1992 and Moncada et al., Pharmacological Review, 43, 109-142, 1991). It also appears that the adverse effects of excess NO production, in particular pathological vasodilation and tissue damage, may result largely from the effects of NO synthesized by the NOS 2.
- NO generated by NOS 2 has been implicated in the pathogenesis of inflammatory diseases.
- hypotension induced by LPS or TNF- ⁇ can be reversed by NOS inhibitors and reinitiated by L-arginine (Kilbourn et. al, PNAS, 87, 3629- 32, 1990).
- Conditions which lead to cytokine-induced hypotension include septic shock, hemodialysis (Beasley and Brenner, Kidney Int., 42, Suppl., 38, S96--S100, 1992) and IL-2 therapy in cancer patients (Hibbs et. al, J. Clin. Invest., 89, 867-77, 1992).
- NOS 2 is implicated in these responses, and thus the possibility exists that a NOS inhibitor would be effective in ameliorating cytokine-induced hypotension.
- Recent studies in animal models have suggested a role for NO in the pathogenesis of inflammation and pain and NOS inhibitors have been shown to have beneficial effects on some aspects of the inflammation and tissue changes seen in models of inflammatory bowel disease, (Miller et. al, J. Pharmacol Exp. Ther., 264, 11-16, 1990) and cerebral ischemia and arthritis (Ialenti et. al, Br. J. Pharmacol ., 110, 701-6, 1993; Stevanovic- Racic et al, Arth. & Rheum., 37, 1062-9, 1994).
- transgenic mice deficient in NOS 1 show diminished cerebral ischemia (Huang et. al, Science, 265, 1883-5, 1994) and transgenic mice deficient in NOS 2 exhibit enhanced survivability in a model of LPS-induced shock (MacMicking et al Cell 81, 641-650, 1995) and Wei et al. Nature 375, 408-411, 1995)). Further conditions where there is an advantage in inhibiting
- NO production from L-arginine include therapy with cytokines such as TNF, IL-1 and IL-2 or therapy with cytokine-inducing agents, for example 5, 6-dimethylxanthenone acetic acid, and as an adjuvant to short term immunosuppression in transplant therapy.
- cytokines such as TNF, IL-1 and IL-2
- cytokine-inducing agents for example 5, 6-dimethylxanthenone acetic acid
- compounds which inhibit NO synthesis may be of use in reducing the NO concentration in patients suffering from inflammatory conditions in which an excess of NO contributes to the pathophysiology of the condition, for example adult respiratory distress syndrome (ARDS) and myocarditis.
- ARDS adult respiratory distress syndrome
- myocarditis myocarditis
- an NO synthase enzyme may be involved in the degeneration of cartilage which takes place in autoimmune and/or inflammatory conditions such as arthritis, rheumatoid arthritis, chronic bowel disease and systemic lupus erythematosis (SLE). It is also thought that an NO synthase enzyme may be involved in insulin- dependent diabetes mellitus. Therefore, a yet further aspect of the present invention provides cyclic amidine derivatives or salts thereof in the manufacture of a medicament for use in cytokine or cytokine-inducing therapy, as an adjuvant to short term immunosuppression in transplant therapy, for the treatment of patients suffering from inflammatory conditions in which an excess of NO contributes to the pathophysiology of the condition.
- nitric oxide synthase mediated diseases and disorders including neurodegenerative disorders, disorders of gastrointestinal motility and inflammation.
- diseases and disorders include hypotension, septic shock, toxic shock syndrome, hemodialysis, IL-2 therapy such as in in cancer patients, cachexia, immunosuppression - 6 -
- autoimmune and/or inflammatory indications including sunburn, eczema or psoriasis and respiratory conditions such as bronchitis, asthma, oxidant-induced lung injury and acute respiratory distress (ARDS), glomerulonephritis, restenosis, inflammatory sequelae of viral infections, myocarditis, heart failure, atherosclerosis, osteoarthritis, rheumatoid arthritis, septic arthritis, chronic or inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic lupus erythematosis (SLE), ocular conditions such as ocular hypertension, retinitis and uveitis, type 1 diabetes, insulin- dependent diabetes mellitus and cystic fibrosis.
- ARDS oxidant-induced lung injury and acute respiratory distress
- glomerulonephritis glomerulonephritis
- restenosis inflammatory sequelae of viral infections
- myocarditis heart failure
- atherosclerosis osteo
- Compounds of Formula I are also usful in the treatment of hypoxia, hyperbaric oxygen convulsions and toxicity, dementia, Alzheimer's disease, Sydenham's chorea, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis (ALS), multiple sclerosis, epilepsy, Korsakoff s disease, imbecility related to cerebral vessel disorder, NO mediated cerebral trauma and related sequelae, ischemic brain edema (stroke), sleeping disorders, eating disorders such as anorexia, schizophrenia, depression, pre-menstrual syndrome (PMS), urinary incontinence, anxiety, drug and alcohol addiction, pain, migraine, emesis, tumor growth, immune complex disease, as immunosupressive agents, acute allograft rejection, infections caused by invasive microorganisms which produce NO and for preventing or reversing tolerance to opiates and diazepines.
- ALS amyotrophic lateral sclerosis
- epilepsy Korsakoff s disease
- side a or side b has a double bond
- X is selected from O, S(0) m , NH, and NR6, wherein R ⁇ is selected from Cl-l2alkyl, Cl-l2alkyl-carbonyl, C 1 - 12alkyloxy-carbonyl, C 1 - 12alkylamino-carbonyl Cl-l2alkyl-sulfonyl and Cl-l2alkylamino-sulfonyl wherein said Cl-12alkyl group being optionally mono or di- substituted by substituents being independently selected phenyl, Ci-6alkoxy, amino, and halo; m is 0, 1 or 2;
- Rl, R2, R3 and R4 are each independently selected from the group consisting of
- hetero C5-l()cycloalkyl wherein the hetero C5-l()cycloalkyl optionally contains 1 or 2 heteroatoms selected from S, O and N, (1) aryl, selected from phenyl or naphthyl, (m) heteroaryl, wherein heteroaryl is selected from the group consisting of:
- aryl-S(0)2NH where aryl is selected from phenyl and naphthyl
- aryl-C(O) where aryl is selected from phenyl, naphthyl, pyridyl, thienyl, thiazolyl, oxazolyl, imidazolyl, and triazolyl
- R7 is selected from hydrogen, phenyl, benzyl, cyclohexyl or Ci-6alkyl,
- each of (b) to (y) being optionally mono or di- substituted, the substituents being independently selected from
- halo selected from F, Cl, Br and I
- hetero C5- 1 Ocycloalkyl wherein the hetero C5-l ⁇ cycloalkyl optionally contains 1 or 2 heteroatoms selected from S, O and N,
- aryl selected from phenyl or naphthyl
- heteroaryl wherein heteroaryl is selected from the group consisting of: (a) imidazolyl,
- R2, 3 an R4 including the optional substituents present thereon reside on the same carbon atom of Formula I, or two of the group Rl , R2, R3 and R4 including the optional substituents present thereon reside on adjacent atoms of Formula I
- said two members may optionally be joined, such that together with the carbon atom to which they are attached there is formed a saturated or unsaturated monocyclic ring of 5, 6 or 7 atoms, said monocyclic ring optionally containing up to three hetero atoms selected from N, O or S, or when a member of the group Rl, R2, R3 and R4 including the optional substituents present thereon resides on an atom adjacent to the N on which R6 resides, said member may optionally be joined with R6, such that together with the N on which R6 resides and the carbon on which said member resides there is formed a saturated or unsaturated monocyclic heterocycle of 5, 6 or 7 atoms, said monocycle optionally containing up to three hetero atoms selected from
- halo selected from F, Cl, Br and I
- hetero C5-l ⁇ cycloalkyl wherein the heteroC5-iocycloalkyl optionally contains 1 or
- aryl selected from phenyl or naphthyl, (g) cyclohexyl.
- Rl, R2, R3 and R4 are each independently selected from the group consisting of
- aryl where aryl is phenyl and naphthyl
- aryloxy where aryl is phenyl and naphthyl
- cycloalkyl wherein the cycloalkyi is a 5-, 6-, or 7-membered monocyclic ring which optionally contains 1 or 2 heteroatoms selected from S, O, and N, and
- heteroaryl wherein heteroaryl is selected from the group consisting of:
- R6R7N-S02-NH-C( 0)-, wherein R6 and R7 are independendy selected from the group consisting of
- each of (b) to (e) being mono- or di- substituted, the substituents being independently selected from (1) hydroxy, (2) Cl-3alkoxy,
- halo selected from fluoro, chloro, bromo, and iodo, (10) oxo, and
- Rl, R2, R3 and R4 are each independently selected from the group consisting of
- R5 is selected from the group consisting of
- R6R7N-S02-NH-C( 0)-, optionally mono or di-substituted, wherein R6 and R7 are independently selected from the group consisting of
- halo selected from fluoro, chloro, and bromo.
- alkyl is defined to include linear, branched, and cyclic structures, with Cl-6alkyl including methyl, ethyl, propyl, 2-propyl, s- and t-butyl, butyl, pentyl, hexyl, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
- Ci-6alkoxy is intended to include alkoxy groups of from 1 to 6 carbon atoms of a straight, branched, or cyclic configuration. Examples of lower alkoxy groups include methoxy, ethoxy, propoxy, isopropoxy, cyclopropyloxy, cyclohexyloxy, and the like.
- Cl-6 alkylthio is intended to include alkylthio groups of from 1 to 6 carbon atoms of a straight, branched or cyclic configuration.
- Examples of lower alkylthio groups include methylthio, propylthio, isopropylthio, cycloheptylthio, etc.
- the propylthio group signifies -SCH2CH2CH3.
- Heteroaryl includes furan, benzofuran, thiophene, py ⁇ ole, indole, isoxazole, isothiazole, pyrazole, oxazole, benzoxazole, thiazole, imidazole, benzimidazole, 1,2,3-oxadiazole, 1,2,3-thiadiazole, 1,2,3- triazole, 1,3,4-oxadiazole, 1,3,4-thiadiazole, 1,3,4-triazole, 1,2,5- oxadiazole, 1,2,5-thiadiazole, pyridine, quinoline, isoquinoline, pyridazine, pyrimidine, pyrazine, 1,2,4-triazine, 1,3,5-triazine, 1,2,4,5- tetrazine, tetrazole. and the like.
- the compounds of the instant invention are useful in the treatment of a number of NOS implicated diseases.
- NOS implicated diseases The implication of these diseases is well documented in the literature.
- psoriasis see Ruzicka et. al, J. Invest. Derm., 103: 397 (1994) or Kolb-Bachofen et. al, Lancet, 344: 139 (1994) or Bull, et al., J. Invest. Derm., 103:435(1994); with regard to uveitis, see Mandia et.
- compositions of the present invention comprise a compound of Formula I as an active ingredient or a pharmaceutically acceptable salt, thereof, and may also contain a pharmaceutically acceptable carrier and optionally other therapeutic ingredients.
- pharmaceutically acceptable salts refers to salts prepared from pharmaceutically acceptable non-toxic acids or bases including inorganic bases and organic bases. Salts derived from inorganic acids include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic salts, manganous, potassium, sodium, zinc, and the like. Particularly preferred are the ammonium, calcium, magnesium, potassium, and sodium salts.
- Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as arginine, betaine, caffeine, choline, N,N_- dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2- dimethylaminoethanol, ethanolamine, ethylenediamine, N- ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine.
- basic ion exchange resins such as arginine, betaine, caffeine, choline, N,N_- dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2- dimethylaminoethanol, ethanolamine, ethylenediamine, N- ethylmorpho
- lysine methylglucar ⁇ ine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.
- compositions containing the active ingredient of the instant invention may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs.
- Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets.
- excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example, magnesium stearate, stearic acid or talc.
- the tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period.
- a time delay material such as glyceryl monostearate or glyceryl distearate may be employed.
- Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin, or olive oil.
- Aqueous suspensions contain the active material in admixture with excipients suitable for the manufacture of aqueous suspensions.
- excipients are suspending agents, for example sodium carboxymethyl-cellulose, methylcellulose, hydroxy- propylmethycellulose, sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethylene- oxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate.
- dispersing or wetting agents may be a naturally-occurring phosphatide, for example le
- the aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl, p- hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose, saccharin or aspartame.
- Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in mineral oil such as liquid paraffin.
- the oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
- Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, may also be present.
- the pharmaceutical compositions of the invention may also be in the form of an oil-in-water emulsions.
- the oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these.
- Suitable emulsifying agents may be naturally-occurring phosphatides, for example soy beans, lecithin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate.
- the emulsions may also contain sweetening and flavouring agents. Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
- the pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleagenous suspension.
- This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above.
- the sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally- acceptable diluent or solvent, for example as a solution in 1,3-butane diol.
- the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
- sterile, fixed oils are conventionally employed as a solvent or suspending medium.
- any bland fixed oil may be employed including synthetic mono- or diglycerides.
- fatty acids such as oleic acid find use in the preparation of injectables.
- compositions may also be administered in the form of a suppositories for rectal administration of the drug.
- These compositions can be prepared by mixing the drug with a suitable non- irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
- suitable non- irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
- Such materials are cocoa butter and polyethylene glycols.
- topical use creams, ointments, jellies, solutions or suspensions, etc., containing the compound of Formula I are employed. (For purposes of this application, topical application shall include mouth washes and gargles.)
- Dosage levels of the order of from about 0.01 mg to about 140 mg/kg of body weight per day are useful in the treatment of the above-indicated conditions, or alternatively about 0.5 mg to about 7 g per patient per day.
- inflammation may be effectively treated by the administration of from about 0.01 to 50 mg of the compound per kilogram of body weight per day, or alternatively about 0.5 mg to about 3.5 g per patient per day, preferably 2.5 mg to 1 g per patient per day.
- the amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.
- a formulation intended for the oral administration of humans may contain from 0.5 mg to 5 g of active agent compounded with an appropriate and convenient amount of carrier material which may vary from about 5 to about 95 percent of the total composition.
- Dosage unit forms will generally contain between from about 1 mg to about 500 mg of an active ingredient, typically 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg, or 1000 mg.
- NOS activity is measured as the formation of L-[2,3,4,5- 3H]Citrulline from L- r 2,3A5- 3 H] Arginine.
- the incubation buffer 100 uL contained; 100 mM TES, pH 7.5, 5 uM FAD, 5 uM FMN, 10 uM BH4, 0.5 mM NADPH, 0.5 mM DTT, 0.5 mg/mL BSA, 2 mM CaC12, 10 ug/mL cahnodulin (bovine), 1 uM L-Arg, 0.2 uCi L-[2,3,4,5- 3 H]Arg, and the inhibitor in aqueous DMSO (max. 5 %).
- the reaction is initiated by addition of enzyme.
- Illustrative of the utility of the compounds of Formula I is the ability of such compounds to inhibit NO synthase as shown in Table 1 and as measured by the assay described above:
- the compounds of the present invention can be prepared according to the following methods.
- Reaction conditions a) H 2 40 psi, Pd(OH)_>/C, EtOH, HOAc, 4 hr; b) (t-C 4 H9 ⁇ 2C) 2 O, NaCl, NaOH, CHCI 3 , reflux, 4hr; c) (CH3)3OBF 4j CH 2 C
- hexahydro-l-(phenylmethyl)-(5/7)- l,4-diazepin-5-one A (prepared as described by T. Irikura, CAS 84:31153r, 83: 179149u) is reacted under hydrogen atmosphere at 40 psi in the presence of palladium hydroxide catalyst in ethanol and acetic acid to give hexahydro-5//-l,4-diazepin-5-one B as the acetic acid salt.
- Reaction conditions a) NH 2 OH - HCl, NaOH, EtOH; b) n-BuLi, TsCl; Et 3 N, aq. dioxane; c) Lawesson's reagent, tol, 90°C; d) Me 3 OBF 4 , iPr 2 NEt, CH 2 CI 2 ; NH 4 CI, EtOH, reflux
- 1,4-Oxa- and thiazepine analogs are prepared by methodology outiine in Scheme 3.
- a ketone derivative A is converted to its corresponding oxime B by reaction with hydroxylamine in ethanol.
- Ring expansion of B via a Beckmann rearrangement of the O-tosyl- oxime formed by reaction of B with with butyl lithium and p-toluenesulfonyl chloride gives hexahydro-l,4-heteroazepin-5-one £,.
- X S
- the amide in Q is converted to the thioamide D by reaction with Lawesson's reagent.
- More highly substituted hexahydro-5-imino-l,4- heteroazepines may be prepared according to methodology outlined in Scheme 4.
- Diester A is cyclized via a Dieckmann condensation to keto- ester B.
- B Treatment of B with a strong base such as sodium hydride followed by addition of an alkylating agent such as n-propyl iodide will give E (where R2 is hydrogen and R3 is n-propyl).
- keto- ester B may be oxidized by manganese dioxide to form the ⁇ , ⁇ - unsaturated keto-ester C.
- a substituent R2 is introduced via a Michael reaction with an organo-cuprate reagent to form D.
- Reaction conditions a) (t-C 4 H9O) 2 CO, NaHCO 3 , CH 2 CI 2 ; b) Ph 3 P, (i-C 3 H7 ⁇ 2 C)2N2, THF; c) HS(CH2) 2 CO 2 H, CsCO 3 , DMF; d) HCl, EtOAc; e) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide,
- More highly substituted hexahydro-5-imino- 1 ,4- heteroazepines may also be prepared according to methodology outlined in Scheme 5. Briefly, the amine functionality in aminoalcohol A is protected to give B. Mitsunobu conditions will cyclize B to form aziridine Q. The aziridine ring in Q is opened with ⁇ -mercaptopropionic acid followed by treatment with hydrochloric acid in ethyl acetate to yield amino acid D. Reaction of D under standard peptide bond forming reactions gives lactam E. Reaction with Lawesson's reagent gives the thiolactam F which is converted to 5-imino-l,4-thiazepine Q_by previously described conditions.
- Step A Hexahvdro-(5/T,- 1.4-diazepine-5-one acetic acid salt.
- l-Benzylhexahydro-(5 )-l,4-diazepine-5-one (1.5 g, 7.34 mmol) was dissolved in 12 mL of ethanol and 6 mL of acetic acid. After addition of 150 mg of 20% palladium hydroxide on carbon, the mixture was shaken under 40 psi of hydrogen for 4 h. The resulting mixture was centrifuged and the supematant was filtered through a 0.45 micron membrane filter. The catalyst was washed with ethanol (3 x 10 mL), and the combined filtrate was concentrated in vacuo to give a yellow oil which began to crystallize.
- Step B 1 -(ferf-ButoxycarbonyDhexahvdro-fS/fl- 1.4-diazepin-5-one.
- Step C l-(?grr-Butoxycarbonyl -2.3.6.7-tetrahvdro-5-methoxy-f IH)- 1 ,4-diazepine.
- Trimethyloxonium tetrafluoroborate (Meerwein's salt) (141 mg, 0.94 mmol) was added in one portion to a solution of l-(tert- butoxycarbonyl)hexahydro-(5 )-l,4-diazepin-5-one (200 mg, 0.94 mmol) in 2.0 mL of anhydrous methylene chloride. The mixture was stirred overnight at room temperature. The reaction mixture was partitioned between 10 mL of saturated aqueous sodium bicarbonate and 20 mL of ethyl acetate. The organic layer was separated and the aqueous layer was extracted with 3 x 10 mL of ethyl acetate.
- Step D 1 -(fe -Butoxycarbonyl hexahvdro-5-imino-( 1 H)- 1.4- diazepine hydrochloride .
- Step E Hexahvdro-5-Imino-( IH)- 1 ,4-diazepine dihydrochloride.
- Step B Tetrahvdro-(2/ifi- 1 ,4-thiazepin-5-one
- Step D Hexahydro-5-imino- 1 ,4-thiazepine hydrochloride
- step A tetrahydropyran-4-one was converted to 4-oximino-tetrahydropyran.
- Step B Tetrahvdro-(2HV 1.4-oxazepin-5-one
- step B 4-oximino- tetrahydropyran was converted to tetrahydro-(2//)-l,4-oxazepin-5-one.
- step D tetrahydro-(2H) 1, oxazepin-5-one was reacted with Meerwien's salt and ammonium chloride to form hexahydro-5-imino-l//-l,4-oxazepine, hydrochloride.
- Step A Tetrahydrothiopyran-4-one-3-carboxylic acid, allyl ester.
- Step B 3-Propyl-tetrahvdrothiopyran-4-one-3-carboxylic acid, allyl ester.
- Step C 3-Propyl-tetrahydrothiopyran-4-one.
- Step D 4-Oximino-3-propyl-tetrahvdrothiopyran.
- step A 3-propyl- tetrahydrothiopyran-4-one was reacted with hydroxylamine hydrochloride to form 4-oximino-3-propyl-tetrahydrothiopyran and was used directly in the subsequent reaction.
- Step E Tetrahvdro-3-propyl-(2 f)- 1 ,4-thiazepin-5-one and tetrahvdro-6-propyl-(2 D- 1.4-thiazepin-5-one.
- step B 4-o ⁇ immo-3- propyl-tetrahydrothiopyran was converted to a 3: 1 mixture of tetrahydro- 3- ⁇ ropyl-(2/7)-l,4-thiazepin-5-one and tetrahydro-6-propyl-(2//)-l,4- thiazepin-5-one.
- Step F Tetrahvdro-3-propyl-(2/f)- 1 ,4-thiazepin-5-thione.
- step C the mixture of tetrahydro-3-propyl-(2 - 1 ,4-thiazepin-5-one and tetrahydro-6-propyl- (2H)-l,4-thiazepin-5-one was reacted with Lawesson's reagent to yield the corresponding thioamides.
- the 3-n-propyl isomer was isolated and purified by flash column chromatography on silica gel eluted with methylene chloride : hexanes (1:1) to yield tetfahydro-3-propyl-(2i/)-l,4- thiazepin-5-thione as a single compound.
- Step G Hexahvdro-5-imino-3-propyl- 1 ,4-thiazepine hydrochloride.
- step D tetrahydro-3- propyl-(2 )-l,4-thiazepin-5-thione was reacted with Meerwein's salt and ammonium chloride to yield hexahydro-5-imino-3-propyl-l,4-thiazepine, hydrochloride.
- Step A 2.3-Dihydrothiopyran-4-one-3-carboxylic acid, allyl ester.
- Step B 2-Methyl-tetrahvdrothiopyran-4-one-3-carboxylic acid, allyl ester.
- Step D Hexahydro-5-imino-7-methyl-1.4-thiazepine hydrochloride.
- Hexahydro-5-imino-2-methyl- 1 ,4-thiazepine hydrochloride was prepared according to the procedures described in Example 6.
- step B the 6- positional isomer was separated from the 3-positional isomer (see Example 9) as its respective thioamide by flash column chromatography
- step D reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro-5-imino-6-(3-methyl-2- n-butenyl)- 1 ,4-thiazepine hydrochloride.
- step B the 3- positional isomer was separated from the 6-positional isomer (see Example 8) by flash column chromatography as its respective thioamide. Subsequently, reaction with Meerwein's salt and ammomum chloride as described in Example 2, step D gave hexahydro-5-imino-3-(3-methyl-2- n-butenyl)- 1 ,4-thiazepine hydrochloride.
- step B the 6-positional isomer was separated from the 3-positional isomer (see Example 11) as its respective thioamide by flash column chromatography . Subsequently, reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro-5-i ⁇ no-6-(2-methyl-propyl)-l,4-thiazepine hydrochloride.
- step B the 3-positional isomer was separated from the 6-positional isomer (see Example 13) as its respective thioamide by flash column chromatography . Subsequently, reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro-5-imino-3-methyl-l,4-thiazepine hydrochloride.
- step B the 3-positional isomer was separated from the 6-positional isomer as its respective thioamide by flash column chromatography . Subsequently, reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro- 5-imino-3-ethyl- 1 ,4-thiazepine hydrochloride.
- step B the 3-positional isomer was separated from the 6-positional isomer as its respective thioamide by flash column chromatography . Subsequently, reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro- 5-imino-3-butyl- 1 ,4-thiazepine, hydrochloride.
- iH NMR 500 MHz, CD3OD: ⁇ 3.90 (m, IH), 3.23 (m, IH), 3.02 (m,
- step B the 3-positional isomer was separated from the 6-positional isomer as its respective thioamide by flash column chromatography . Subsequently, reaction with Meerwein's salt and ammonium chloride as described in Example 2, step D gave hexahydro-5-imino-3-(2-methyl-3-propenyl)-l,4-thiazepine hydrochloride.
- (+)-frfln5-Decahvdro-4-imino-benzorbl-1.4-thiazepine acetic acid salt (+)-frfln5-Decahvdro-4-imino-benzorbl-1.4-thiazepine acetic acid salt.
- Step B ( ⁇ V7-(fgrt-ButoxycarponylV7-aza-bicvclo-14.1.01- cycloheptane
- Step C ( ⁇ frQ «.y-2-amino-l-12-(carboxy)ethylthio1-cyclohexane hydrochloride
- Step D ( ⁇ r. s-Decahydro-4-oxo-benzolbl-1.4-thiazepine
- reaction mixture After stirring for an additional 5 mins., the reaction mixture was warmed to room temperature and stirred ovemight at the same temperature. The following day the reaction mixture was diluted with water and extracted with methylene chloride. The solvent layer was washed with brine, dried and evaporated to give the cmde which was purified by silica column and eluted with hexane/ethylacetate (7 : 3 + 5% methanol) to give 0.102 g (55%) of ( ⁇ )- f r ⁇ ns-decahydro-4-oxo-benzo[b]- 1 ,4-thiazepane as white solid.
- Step E ( ⁇ Vfr ⁇ n -Decahvdro-4-thioxo-benzolbl-1.4-thiazepine
- Step F ( ⁇ ran.s-Decahvdro-4-imino-benzorbl- 1.4-thiazepine acetic acid salt.
- ( ⁇ )- ran5-decahydr ⁇ -4-thioxo-benzo[b]-l,4-thiazepane 25 mg, 0.12 mmol
- trimethyloxonium tetrafluoroborate Meerwein's salt
- Step A Tetrahvdro-3(S)-propyl-(2 D- 1.4-thiazepine-5-thione
- the title compound was prepared employing the procedure in Example 17, Steps A to E and starting from L-norvalinol instead of ( ⁇ )-trans-2- aminocy clohexanol .
- Step B Hexahvdro-5-imino-3(S Vpropyl- 1 ,4-thiazepine acetic acid salt
- the cmde compound was then purified by column chromatography and eluted with acetonitrile : water : acetic acid (90:5:5) giving 31.5 mg of hexahydro-5-imino-3(S)- ⁇ ropyl-l,4-thiazepine acetic acid salt.
- Step B Hexahvdro-5-imino-3(RVpropyl- 1.4-thiazepine acetic salt
- step B tetrahydro-3(R)-propyl-(2 /)-l,4- thiaze ⁇ ine-5-thione (40 mg, 0.21 mmol) was converted to 37.4 mg of hexahydro-5-imino-3(R)-propyl-l,4-thiazepine acetic acid salt.
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Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002234641A CA2234641A1 (en) | 1995-11-01 | 1996-10-28 | Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
AU75287/96A AU700078B2 (en) | 1995-11-01 | 1996-10-28 | Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
EP96937836A EP0861238A4 (en) | 1995-11-01 | 1996-10-28 | Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US717295P | 1995-11-01 | 1995-11-01 | |
US60/007,172 | 1995-11-01 | ||
US901295P | 1995-12-21 | 1995-12-21 | |
US60/009,012 | 1995-12-21 | ||
GBGB9605162.8A GB9605162D0 (en) | 1996-03-12 | 1996-03-12 | Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
GBGB9605700.5A GB9605700D0 (en) | 1996-03-19 | 1996-03-19 | Hexahydro-5-imino,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
GB9605700.5 | 1996-03-19 | ||
GB9605162.8 | 1996-03-19 |
Publications (1)
Publication Number | Publication Date |
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WO1997016430A1 true WO1997016430A1 (en) | 1997-05-09 |
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ID=27451425
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Application Number | Title | Priority Date | Filing Date |
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PCT/US1996/017447 WO1997016430A1 (en) | 1995-11-01 | 1996-10-28 | Hexahydro-5-imino-1,4-heteroazepine derivatives as inhibitors of nitric oxide synthases |
Country Status (5)
Country | Link |
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EP (1) | EP0861238A4 (en) |
JP (1) | JP3188715B2 (en) |
AU (1) | AU700078B2 (en) |
CA (1) | CA2234641A1 (en) |
WO (1) | WO1997016430A1 (en) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
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US5854234A (en) * | 1993-10-21 | 1998-12-29 | G. D. Searle & Co. | Amidino dervatives useful as nitric oxide synthase inhibitors |
US5883251A (en) * | 1995-04-20 | 1999-03-16 | G. D. Searle & Co. | Azepine derivatives useful as nitric oxide synthase inhibitors |
WO1999041240A1 (en) * | 1998-02-12 | 1999-08-19 | Schering Aktiengesellschaft | 3,4-dihydroquinoline derivatives as nitrogen monoxide synthase (nos) inhibitors |
US5958958A (en) * | 1997-07-22 | 1999-09-28 | G.D. Searle & Co. | 1,2,4-oxa diazolino and 1,24-oxa diazolidion heterocycles as useful nitric oxide synthase inhibitors |
EP1036565A1 (en) * | 1997-12-05 | 2000-09-20 | Institute of Medicinal Molecular Design, Inc. | Preventives/remedies for diabetes |
WO2000064904A1 (en) * | 1999-04-28 | 2000-11-02 | Astrazeneca Ab | 5,7-bicyclic amidine derivatives useful as nitric oxide synthase inhibitors |
US6344473B1 (en) | 2000-08-07 | 2002-02-05 | G.D. Searle & Co. | Imidazoles useful as nitric oxide synthase inhibitors |
US6489323B1 (en) | 1998-06-10 | 2002-12-03 | G.D. Searle & Co. | Heterobicyclic and tricyclic nitric oxide synthase inhibitors |
EP2085399A1 (en) | 2008-01-29 | 2009-08-05 | Sanofi-Aventis | substituted arylamide oxazepinopyrimidone derivatives |
WO2009095792A2 (en) | 2008-01-29 | 2009-08-06 | Sanofi-Aventis | Substituted heteroarylamide diazepinopyrimidone derivatives |
EP2090578A1 (en) | 2008-01-29 | 2009-08-19 | Sanofi-Aventis | Substituted arylamide diazepinopyrimidone derivatives for the treatment of neurodegenerative diseases caused by abnormal activity of GSK3-beta |
US8202874B2 (en) | 2008-06-26 | 2012-06-19 | Mitsubishi Tanabe Pharma Corporation | Substituted N-oxide pyrazine derivatives |
US8211903B2 (en) | 2008-06-26 | 2012-07-03 | Mitsubishi Tanabe Pharma Corporation | Substituted pyrimido isoquinoline derivatives |
US8273758B2 (en) | 2008-06-26 | 2012-09-25 | Sanofi | Substituted tricyclic derivatives |
US8501728B2 (en) | 2008-01-29 | 2013-08-06 | Sanofi | Substituted heteroarylamide oxazepinopyrimidone derivatives |
US8592580B2 (en) | 2008-06-26 | 2013-11-26 | Sanofi | Substituted triazinone derivatives |
US8614219B2 (en) | 2008-06-26 | 2013-12-24 | Sanofi-Aventis | Substituted pyrimidin-4-one derivatives |
US9073914B2 (en) | 2008-06-26 | 2015-07-07 | Sanofi | Substituted alkyl pyrimidin-4-one derivatives |
US9073915B2 (en) | 2008-06-26 | 2015-07-07 | Sanofi | Substituted pyrimidone derivatives |
CN114787139A (en) * | 2019-11-26 | 2022-07-22 | 吉利德科学公司 | Processes and intermediates for preparing MCL1 inhibitors |
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AT278805B (en) * | 1966-01-17 | 1970-02-10 | American Cyanamid Co | PROCESS FOR THE PREPARATION OF OXACEPINES AND THIAZEPINES |
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US4761411A (en) * | 1983-05-18 | 1988-08-02 | Hoechst-Roussel Pharmaceuticals Inc. | Dihydrobenzopyrrolobenzodiazepines useful for treating pyschoses |
ATE177078T1 (en) * | 1993-10-21 | 1999-03-15 | Searle & Co | AMIDINO DERIVATIVES AS NO-SYNTHETASE INHIBITORS |
US5629322A (en) * | 1994-11-15 | 1997-05-13 | Merck & Co., Inc. | Cyclic amidine analogs as inhibitors of nitric oxide synthase |
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1996
- 1996-10-28 CA CA002234641A patent/CA2234641A1/en not_active Abandoned
- 1996-10-28 JP JP51753197A patent/JP3188715B2/en not_active Expired - Fee Related
- 1996-10-28 EP EP96937836A patent/EP0861238A4/en not_active Withdrawn
- 1996-10-28 AU AU75287/96A patent/AU700078B2/en not_active Ceased
- 1996-10-28 WO PCT/US1996/017447 patent/WO1997016430A1/en not_active Application Discontinuation
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US4096140A (en) * | 1974-11-29 | 1978-06-20 | Sandoz, Inc. | 5-Amino or substituted amino-7-phenyl or substituted phenyl-2,3-dihydro-1H-1,4-diazepines |
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Cited By (31)
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Also Published As
Publication number | Publication date |
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CA2234641A1 (en) | 1997-05-09 |
EP0861238A4 (en) | 2000-03-01 |
JPH11501327A (en) | 1999-02-02 |
EP0861238A1 (en) | 1998-09-02 |
AU700078B2 (en) | 1998-12-17 |
JP3188715B2 (en) | 2001-07-16 |
AU7528796A (en) | 1997-05-22 |
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