WO1996041009A1 - Restes permettant de lier des troisiemes brins a des complexes doubles nucleotidiques complementaires de n'importe quelle sequence paire de base - Google Patents

Restes permettant de lier des troisiemes brins a des complexes doubles nucleotidiques complementaires de n'importe quelle sequence paire de base Download PDF

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Publication number
WO1996041009A1
WO1996041009A1 PCT/US1996/009428 US9609428W WO9641009A1 WO 1996041009 A1 WO1996041009 A1 WO 1996041009A1 US 9609428 W US9609428 W US 9609428W WO 9641009 A1 WO9641009 A1 WO 9641009A1
Authority
WO
WIPO (PCT)
Prior art keywords
residue
backbone
oligonucleotide
bases
strand
Prior art date
Application number
PCT/US1996/009428
Other languages
English (en)
Inventor
Jacques R. Fresco
Bin Lin
Lynn C. Klotz
Original Assignee
Princeton University
Oncorpharm, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University, Oncorpharm, Inc. filed Critical Princeton University
Priority to AU62601/96A priority Critical patent/AU6260196A/en
Priority to EP96921358A priority patent/EP0871772A4/fr
Publication of WO1996041009A1 publication Critical patent/WO1996041009A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays

Abstract

La présente invention concerne des règles et des principes permettant de concevoir des bases hétérocycliques qui, lorsqu'elles sont incorporées dans des oligonucléotides, permettent à ces oligonucléotides de servir d'acides nucléiques de troisième brin capable d'une liaison spécifique avec les acides nucléiques à double brin de n'importe quelle séquence paire de base, sans qu'il y ait besoin de brin riche en purine. Les bases servant d'exemple de la figure ont été conçues en mettant en ÷uvre le procédé de l'invention.
PCT/US1996/009428 1995-06-07 1996-06-06 Restes permettant de lier des troisiemes brins a des complexes doubles nucleotidiques complementaires de n'importe quelle sequence paire de base WO1996041009A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU62601/96A AU6260196A (en) 1995-06-07 1996-06-06 Residues for binding third strands to complementary nucleic acid duplexes of any base pair sequence
EP96921358A EP0871772A4 (fr) 1995-06-07 1996-06-06 Restes permettant de lier des troisiemes brins a des complexes doubles nucleotidiques complementaires de n'importe quelle sequence paire de base

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/473,888 1995-06-07
US08/473,888 US6426407B1 (en) 1995-06-07 1995-06-07 Residues for binding third strands to complementary nucleic acid duplexes of any base pair sequence

Publications (1)

Publication Number Publication Date
WO1996041009A1 true WO1996041009A1 (fr) 1996-12-19

Family

ID=23881431

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/009428 WO1996041009A1 (fr) 1995-06-07 1996-06-06 Restes permettant de lier des troisiemes brins a des complexes doubles nucleotidiques complementaires de n'importe quelle sequence paire de base

Country Status (4)

Country Link
US (1) US6426407B1 (fr)
EP (1) EP0871772A4 (fr)
AU (1) AU6260196A (fr)
WO (1) WO1996041009A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024622A1 (fr) * 1997-11-10 1999-05-20 Princeton University Hybridation in-situ triplex
WO2012011114A3 (fr) * 2010-07-22 2012-07-26 Genearrest Ltd Composés de liaison à l'adn/arn double brin spécifiques de certaines séquences et leurs utilisations

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7309569B2 (en) * 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US7220541B2 (en) * 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
CA2531869A1 (fr) * 2003-04-30 2004-11-18 Pharmacia Corporation Composes comportant une fraction bicyclique fusionnee qui se lie au petit sillon d'adn double brin

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009705A1 (fr) * 1990-11-23 1992-06-11 Gilead Sciences, Inc. Oligomeres formant un triplex et contenant des bases modifiees
US5176996A (en) * 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5352580A (en) * 1993-05-26 1994-10-04 Becton, Dickinson And Company Selective detection of mycobacteria by nucleicacid probes derived from Mycobacterium kansasii

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422251A (en) * 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
WO1992010590A1 (fr) * 1990-12-10 1992-06-25 Gilead Sciences, Inc. Inhibition de transcription par formation de triple helice
JP3495752B2 (ja) * 1992-12-11 2004-02-09 キヤノン株式会社 生標的個体の検出方法およびプローブ

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176996A (en) * 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
WO1992009705A1 (fr) * 1990-11-23 1992-06-11 Gilead Sciences, Inc. Oligomeres formant un triplex et contenant des bases modifiees
US5352580A (en) * 1993-05-26 1994-10-04 Becton, Dickinson And Company Selective detection of mycobacteria by nucleicacid probes derived from Mycobacterium kansasii

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ANN. REV. BIOPHYS. BIOMOL. STRUCT., 1995, Vol. 24, PLUM et al., "Nucleic Acid Hybridization: Triplex Stability and Energetics", pages 319-350. *
BIOCHEM. SOC. TRANS., October 1989, Vol. 17, No. 5, VOSS et al., "Use of 2,6-Diaminopurine in Oligonucleotide Gene Probes", page 913. *
BIOPOLYMERS, 1993, Vol. 33, MOHAN et al., "Molecular Recognition of Watson Crick Base-Pair Reversals in Triple Helix Formation: Use of Nonnatural Oligonucleotide Bases", pages 1317-1325. *
BIOPOLYMERS, 1995, Vol. 35, CHENG et al., "Solvent Effects on Model d(CG G)7 and d(TA T)7 DNA Triple Helices", pages 457-473. *
J. AM. CHEM. SOC., 1994, Vol. 116, XIANG et al., "A New Pyrimidine Nucleoside (m50xC) for the pH-Independent Recognition of G-C Base Pairs by Oligonucleotide-Directed Triplex Formation", pages 11155-11156. *
MOL. ENGINEER., 1995, Vol. 5, SUN et al., "Rational Design of Switched Triple Helix Forming Oligonucleotides: Extension of Sequences for Triple Helix Formation", pages 157-178. *
NUCLEIC ACIDS RES., 1988, Vol. 16, No. 1, CHOLLET et al., "DNA Containing the Base Analogue 2-Aminoadenine: Preparation, Use as Hybridization Probes and Cleavage by Restriction Enzymes", pages 305-317. *
NUCLEIC ACIDS RES., 1988, Vol. 16, No. 11, CHEONG et al., "Thermodynamic Studies of Base Pairing Involving 2,6-Diaminopurine", pages 5115-5122. *
NUCLEOSIDES NUCLEOTIDES, 1994, Vol. 13, No. 1-3, CANO et al., "Synthesis of Oligodeoxyribonucleotides Containing 2,6-Diaminopurine", pages 501-509. *
See also references of EP0871772A4 *
SEKHARUDU et al., "DNA Triple Helices with Reverse-Hoogsteen and Purine-Purine Hydrogen Bonding: A Molecular Dynamics Study", In: STRUCTURAL BIOLOGY: THE STATE OF ART, PROCEEDINGS OF THE EIGHT CONVERSATION, Edited by R. SARMA et al., NEW YORK: ADENINE PRESS, 1994, pages 113-125. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024622A1 (fr) * 1997-11-10 1999-05-20 Princeton University Hybridation in-situ triplex
WO2012011114A3 (fr) * 2010-07-22 2012-07-26 Genearrest Ltd Composés de liaison à l'adn/arn double brin spécifiques de certaines séquences et leurs utilisations

Also Published As

Publication number Publication date
EP0871772A1 (fr) 1998-10-21
AU6260196A (en) 1996-12-30
EP0871772A4 (fr) 2002-09-04
US6426407B1 (en) 2002-07-30

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