WO1996040925A3 - Regulatory elements conferring tapetum specificity - Google Patents

Regulatory elements conferring tapetum specificity Download PDF

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Publication number
WO1996040925A3
WO1996040925A3 PCT/US1996/010217 US9610217W WO9640925A3 WO 1996040925 A3 WO1996040925 A3 WO 1996040925A3 US 9610217 W US9610217 W US 9610217W WO 9640925 A3 WO9640925 A3 WO 9640925A3
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WO
WIPO (PCT)
Prior art keywords
tapetum
specific regulatory
regulatory element
regulatory elements
anther
Prior art date
Application number
PCT/US1996/010217
Other languages
French (fr)
Other versions
WO1996040925A2 (en
Inventor
Gary A Huffman
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Priority to AU61745/96A priority Critical patent/AU696222B2/en
Priority to EP96919394A priority patent/EP0835310B1/en
Priority to AT96919394T priority patent/ATE256742T1/en
Priority to JP8536010A priority patent/JPH11506321A/en
Priority to DE69631137T priority patent/DE69631137D1/en
Publication of WO1996040925A2 publication Critical patent/WO1996040925A2/en
Publication of WO1996040925A3 publication Critical patent/WO1996040925A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S47/00—Plant husbandry
    • Y10S47/01—Methods of plant-breeding and including chromosome multiplication

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Superconductors And Manufacturing Methods Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A 94 base pair DNA regulatory element that confers anther tapetum-specific gene expression has been discovered, isolated, and characterized. Additional tapetum-specific regulatory elements have been developed by mutating the 94 base pair anther box. These tapetum-specific regulatory elements can be used to control the expression of a foreign gene that disrupts the function of tapetal cells. Similarly, the combination of at least one tapetum-specific regulatory element and at least one anther-specific regulatory element can be used to disrupt pollen production in anthers. Thus, the control of maize pollen production can be achieved by using a tapetum-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants.
PCT/US1996/010217 1995-06-07 1996-06-07 Regulatory elements conferring tapetum specificity WO1996040925A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU61745/96A AU696222B2 (en) 1995-06-07 1996-06-07 Regulatory elements conferring tapetum specificity
EP96919394A EP0835310B1 (en) 1995-06-07 1996-06-07 Regulatory elements conferring tapetum specificity
AT96919394T ATE256742T1 (en) 1995-06-07 1996-06-07 REGULATORY ELEMENT FOR CONVEYING WALLPAPER SPECIFICITY
JP8536010A JPH11506321A (en) 1995-06-07 1996-06-07 Control elements that give tapetum specificity
DE69631137T DE69631137D1 (en) 1995-06-07 1996-06-07 REGULATORY ELEMENT FOR COMMUNICATING TAPETUM SPECIFICITY

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/477,978 1995-06-07
US08/477,978 US5837850A (en) 1994-04-21 1995-06-07 Regulatory element conferring tapetum specificity

Publications (2)

Publication Number Publication Date
WO1996040925A2 WO1996040925A2 (en) 1996-12-19
WO1996040925A3 true WO1996040925A3 (en) 1997-05-01

Family

ID=23898076

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/010217 WO1996040925A2 (en) 1995-06-07 1996-06-07 Regulatory elements conferring tapetum specificity

Country Status (11)

Country Link
US (1) US5837850A (en)
EP (1) EP0835310B1 (en)
JP (1) JPH11506321A (en)
AR (1) AR002116A1 (en)
AT (1) ATE256742T1 (en)
AU (1) AU696222B2 (en)
CA (1) CA2223459A1 (en)
DE (1) DE69631137D1 (en)
HU (1) HUP9901073A3 (en)
WO (1) WO1996040925A2 (en)
ZA (1) ZA964716B (en)

Families Citing this family (31)

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US5962769A (en) * 1995-06-07 1999-10-05 Pioneer Hi-Bred International, Inc. Induction of male sterility in plants by expression of high levels of avidin
US6037523A (en) * 1997-06-23 2000-03-14 Pioneer Hi-Bred International Male tissue-preferred regulatory region and method of using same
AR021056A1 (en) * 1998-11-03 2002-06-12 Syngenta Participations Ag DNA THAT INCLUDES A SPECIFIC GENE OF RICE AND TRANSGENIC PLANT TRANSFORMED WITH THE SAME
GB9910796D0 (en) * 1999-05-10 1999-07-07 Biogemma Uk Ltd Dna
WO2001019976A2 (en) 1999-09-16 2001-03-22 Monsanto Technology Llc Plant regulatory sequences for control of gene expression
EP1255825A2 (en) * 2000-02-15 2002-11-13 Pioneer Hi-Bred International, Inc. Male tissue-specific regulatory region and method of using same
WO2001083790A2 (en) * 2000-05-01 2001-11-08 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression
WO2002000905A2 (en) * 2000-06-28 2002-01-03 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression
ATE548458T1 (en) * 2000-09-26 2012-03-15 Pioneer Hi Bred Int MALE FERTILITY-MEDIATING NUCLEOTIDE SEQUENCES AND THEIR USE
US7517975B2 (en) * 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7612251B2 (en) * 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
EP2272965A3 (en) 2001-07-06 2011-01-19 Monsanto Technology LLC Methods for enhancing segregation of transgenes in plants and compositions thereof
US20100031387A1 (en) * 2002-02-07 2010-02-04 Hybrigene, Inc. Prevention of transgene escape in genetically modified perennials
US20050044596A1 (en) * 2003-03-19 2005-02-24 Smith Alan G. Methods to confer enhanced floral properties to plants
NZ571825A (en) 2003-12-16 2011-01-28 Pioneer Hi Bred Int Dominant gene suppression transgenes and methods of using same
US20080244765A1 (en) * 2004-12-16 2008-10-02 Pioneer Hi-Bred International, Inc. Methods and compositions for pollination disruption
CA2614915A1 (en) 2005-05-25 2006-11-30 Pioneer Hi-Bred International, Inc. Methods for improving crop plant architecture and yield
US7910802B2 (en) * 2007-08-03 2011-03-22 Pioneer Hi-Bred International, Inc. MSCA1 nucleotide sequences impacting plant male fertility and method of using same
US7919676B2 (en) * 2007-08-03 2011-04-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
US7915478B2 (en) 2007-08-03 2011-03-29 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
CA2715564C (en) * 2008-02-14 2018-01-09 E. I. Du Pont De Nemours And Company Plant genomic dna flanking spt event and methods for identifying spt event
EP2257076B1 (en) * 2009-05-28 2015-02-25 Advanced Digital Broadcast S.A. Video data signal, system and method for controlling shutter glasses
EP2825551A1 (en) 2012-03-13 2015-01-21 Pioneer Hi-Bred International Inc. Genetic reduction of male fertility in plants
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
BR112015023304A2 (en) 2013-03-14 2017-07-18 Pioneer Hi Bred Int method to improve abiotic stress tolerance of a plant, plant and seed
CN105143454A (en) 2013-03-15 2015-12-09 先锋国际良种公司 Compositions and methods of use of ACC oxidase polynucleotides and polypeptides
CA3221516A1 (en) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
WO2016100309A1 (en) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
CA3016487A1 (en) 2016-03-18 2017-09-21 Marc C. Albertsen Methods and compositions for producing clonal, non-reduced, non-recombined gametes
AU2018347545A1 (en) 2017-10-13 2020-04-16 Pioneer Hi-Bred International, Inc. Systems and methods for cellular reprogramming of a plant cell

Citations (14)

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Publication number Priority date Publication date Assignee Title
EP0344029A1 (en) * 1988-04-28 1989-11-29 Plant Genetic Systems, N.V. Plants with modified stamen cells
WO1990008830A1 (en) * 1989-01-26 1990-08-09 Imperial Chemical Industries Plc Hybrid seed production
EP0412911A1 (en) * 1989-08-10 1991-02-13 Plant Genetic Systems, N.V. Plants with modified flowers
WO1992005261A1 (en) * 1990-09-21 1992-04-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Improvements in or relating to organic compounds
WO1992009696A1 (en) * 1990-11-23 1992-06-11 Plant Genetic Systems, N.V. Process for transforming monocotyledonous plants
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WO1993025695A1 (en) * 1992-06-12 1993-12-23 Plant Genetic Systems N.V. Maintenance of male-sterile plants
EP0578611A1 (en) * 1992-07-02 1994-01-12 Ciba-Geigy Ag Anther-specific cDNA sequences, genomic DNA sequences and recombinant DNA sequences
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Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0344029A1 (en) * 1988-04-28 1989-11-29 Plant Genetic Systems, N.V. Plants with modified stamen cells
WO1990008830A1 (en) * 1989-01-26 1990-08-09 Imperial Chemical Industries Plc Hybrid seed production
EP0412911A1 (en) * 1989-08-10 1991-02-13 Plant Genetic Systems, N.V. Plants with modified flowers
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WO1993025695A1 (en) * 1992-06-12 1993-12-23 Plant Genetic Systems N.V. Maintenance of male-sterile plants
EP0578611A1 (en) * 1992-07-02 1994-01-12 Ciba-Geigy Ag Anther-specific cDNA sequences, genomic DNA sequences and recombinant DNA sequences
WO1994021804A1 (en) * 1993-03-18 1994-09-29 Pioneer-Hi-Bred International, Inc. Methods and compositions for controlling plant development
WO1995029247A1 (en) * 1994-04-21 1995-11-02 Pioneer Hi-Bred International, Inc. Regulatory element conferring tapetum specificity
WO1996017945A1 (en) * 1994-12-08 1996-06-13 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
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Title
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Also Published As

Publication number Publication date
US5837850A (en) 1998-11-17
DE69631137D1 (en) 2004-01-29
HUP9901073A3 (en) 2001-11-28
CA2223459A1 (en) 1996-12-19
HUP9901073A2 (en) 1999-07-28
JPH11506321A (en) 1999-06-08
ATE256742T1 (en) 2004-01-15
EP0835310B1 (en) 2003-12-17
AU6174596A (en) 1996-12-30
AR002116A1 (en) 1998-01-07
WO1996040925A2 (en) 1996-12-19
EP0835310A2 (en) 1998-04-15
ZA964716B (en) 1997-12-08
AU696222B2 (en) 1998-09-03

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