WO1996027023A3 - Procede d'utilisation de sites d'amorçage mobiles pour le sequençage de l'adn - Google Patents

Procede d'utilisation de sites d'amorçage mobiles pour le sequençage de l'adn Download PDF

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Publication number
WO1996027023A3
WO1996027023A3 PCT/US1995/016953 US9516953W WO9627023A3 WO 1996027023 A3 WO1996027023 A3 WO 1996027023A3 US 9516953 W US9516953 W US 9516953W WO 9627023 A3 WO9627023 A3 WO 9627023A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna sequencing
priming sites
mobile
mobile priming
prinkers
Prior art date
Application number
PCT/US1995/016953
Other languages
English (en)
Other versions
WO1996027023A2 (fr
Inventor
Jack T Leonard
Original Assignee
Amicon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amicon Inc filed Critical Amicon Inc
Priority to GB9617625A priority Critical patent/GB2302541A/en
Priority to DE19581516T priority patent/DE19581516T1/de
Priority to AU49623/96A priority patent/AU4962396A/en
Publication of WO1996027023A2 publication Critical patent/WO1996027023A2/fr
Publication of WO1996027023A3 publication Critical patent/WO1996027023A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une amorce oligonucléotide présentant une séquence nucléotide sélectionnée à partir du groupe constitué de 5'-CCCCCACCTCCTgCCCATCAT-3' et 5'-gCCCgACCCCACCTCCACTAC-3' et de toute séquence suffisamment homologue à ces séquences qui permettent d'amorcer la réaction de polymérase d'ADN prenant son origine dans certains 'finalisateurs' oligonucléotides à deux brins. L'invention concerne également des procédés d'utilisation de ces oligonucléotides pour le séquencement de l'ADN.
PCT/US1995/016953 1994-12-23 1995-12-22 Procede d'utilisation de sites d'amorçage mobiles pour le sequençage de l'adn WO1996027023A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB9617625A GB2302541A (en) 1994-12-23 1995-12-22 Method of using mobile priming sites for DNA sequencing
DE19581516T DE19581516T1 (de) 1994-12-23 1995-12-22 Verfahren zur Verwendung mobiler Priming-Stellen zur DNA-Sequenzierung
AU49623/96A AU4962396A (en) 1994-12-23 1995-12-22 Method of using mobile priming sites for dna sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36552994A 1994-12-23 1994-12-23
US08/365,529 1994-12-23

Publications (2)

Publication Number Publication Date
WO1996027023A2 WO1996027023A2 (fr) 1996-09-06
WO1996027023A3 true WO1996027023A3 (fr) 1997-01-09

Family

ID=23439240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/016953 WO1996027023A2 (fr) 1994-12-23 1995-12-22 Procede d'utilisation de sites d'amorçage mobiles pour le sequençage de l'adn

Country Status (4)

Country Link
AU (1) AU4962396A (fr)
DE (1) DE19581516T1 (fr)
GB (1) GB2302541A (fr)
WO (1) WO1996027023A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001582A1 (fr) * 1992-07-13 1994-01-20 Medical Research Council Procede de categorisation de populations de sequences nucleotidiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001582A1 (fr) * 1992-07-13 1994-01-20 Medical Research Council Procede de categorisation de populations de sequences nucleotidiques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AKANE, A. ET AL.: "direct dideoxy sequencing of genomic DNA by ligation mediation", BIOTECHNIQUES, vol. 16, no. 2, February 1994 (1994-02-01), US, pages 238 - 41, XP000420385 *
HETZER, M. ET AL.: "PCR mediated analysis of RNA sequences", NUCLEIC ACIDS RESEARCH, vol. 21, no. 3, 1993, GB, pages 5526 - 27, XP002018646 *
KALISCH, B. ET AL: "covalent linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments", GENE, vol. 44, 1986, NL, pages 263 - 270, XP002018647 *
PALITTAPONGARNPIM, P. ET AL.: "DNA fingerprinting of mycobacterium tuberculosis isolates by ligation-mediated polymerase chain reaction", NUCLEIC ACIDS RESEARCH, vol. 21, no. 3, February 1993 (1993-02-01), GB, pages 761 - 62, XP000343308 *
ROSENTHAL, A. ET AL.: "genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction", NUCLEIC ACIDS RESEARCH, vol. 18, no. 10, 25 May 1990 (1990-05-25), GB, pages 3095 - 96, XP000132495 *

Also Published As

Publication number Publication date
AU4962396A (en) 1996-09-18
GB2302541A (en) 1997-01-22
WO1996027023A2 (fr) 1996-09-06
GB9617625D0 (en) 1996-11-06
DE19581516T1 (de) 1997-05-07

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