GB2302541A - Method of using mobile priming sites for DNA sequencing - Google Patents

Method of using mobile priming sites for DNA sequencing

Info

Publication number
GB2302541A
GB2302541A GB9617625A GB9617625A GB2302541A GB 2302541 A GB2302541 A GB 2302541A GB 9617625 A GB9617625 A GB 9617625A GB 9617625 A GB9617625 A GB 9617625A GB 2302541 A GB2302541 A GB 2302541A
Authority
GB
United Kingdom
Prior art keywords
dna sequencing
priming sites
mobile
mobile priming
prinkers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB9617625A
Other versions
GB9617625D0 (en
Inventor
Jack T Leonard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amicon Corp
Original Assignee
Amicon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amicon Corp filed Critical Amicon Corp
Publication of GB9617625D0 publication Critical patent/GB9617625D0/en
Publication of GB2302541A publication Critical patent/GB2302541A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An oligonucleotide primer having a nucleotide sequence selected from the group consisting of 5'-CCCCCACCTCCTgCCCATCAT-3' and 5'-gCCCgACCCCACCTCCACTAC-3' and any sequence sufficiently homologous to these sequences which allow priming of a DNA polymerase reaction initiating from certain double-stranded oligonucleotide "prinkers". Methods of use of these oligonucleotides in DNA sequencing are also disclosed.
GB9617625A 1994-12-23 1995-12-22 Method of using mobile priming sites for DNA sequencing Withdrawn GB2302541A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36552994A 1994-12-23 1994-12-23
PCT/US1995/016953 WO1996027023A2 (en) 1994-12-23 1995-12-22 Method of using mobile priming sites for dna sequencing

Publications (2)

Publication Number Publication Date
GB9617625D0 GB9617625D0 (en) 1996-11-06
GB2302541A true GB2302541A (en) 1997-01-22

Family

ID=23439240

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9617625A Withdrawn GB2302541A (en) 1994-12-23 1995-12-22 Method of using mobile priming sites for DNA sequencing

Country Status (4)

Country Link
AU (1) AU4962396A (en)
DE (1) DE19581516T1 (en)
GB (1) GB2302541A (en)
WO (1) WO1996027023A2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations

Also Published As

Publication number Publication date
DE19581516T1 (en) 1997-05-07
GB9617625D0 (en) 1996-11-06
AU4962396A (en) 1996-09-18
WO1996027023A3 (en) 1997-01-09
WO1996027023A2 (en) 1996-09-06

Similar Documents

Publication Publication Date Title
EP1498494A3 (en) Method of nucleic acid sequencing
UA49843C2 (en) Oligonucleotide for amplification of nucleic acid sequences, method for amplification of a directed nucleic acid sequence, set for performing reaction of amplification of nucleic acid
DK0868530T3 (en) The cascade-nucleinsyreamplificeringsreaktion
WO2001042493A3 (en) Method for the parallel detection of the degree of methylation of genomic dna
CA2223729A1 (en) Universal primer sequence for multiplex dna amplification
GB9901475D0 (en) A method of DNA sequencing
NZ332753A (en) Artificial mismatch hybridization
IL148091A0 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2153898A1 (en) Method for generating single-stranded dna molecules
EP0449972A4 (en) Triple stranded nucleic acid and methods of use
ZA964057B (en) Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers.
WO2001047944A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
IL102765A (en) Reagents and methods to detect and determine mycobacterium nucleic acids
GB9620075D0 (en) Method
EP1212449A4 (en) Method for amplifying signal-flanking sequences from unknown genomic dna
WO1998054313A3 (en) Dna methyltransferase genomic sequences and antisense oligonucleotides
WO2000029623A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
IL145588A0 (en) Amplification and sequencing primer pairs and use thereof
AU7614696A (en) Integrin-linked kinase, its inhibitors and methods of medical treatment using these inhibitors, gene therapy and pseudo-substrate inhibitors
WO1996015264A3 (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
GB2302541A (en) Method of using mobile priming sites for DNA sequencing
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
ATE211180T1 (en) METHOD FOR THE NON-RADIOACTIVE DETECTION OF MEMBRANE-BONDED NUCLEIC ACIDS AND TEST KIT
WO2001040521A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
WO2001048245A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)