WO1996025513A1 - Capteur biochimique - Google Patents
Capteur biochimique Download PDFInfo
- Publication number
- WO1996025513A1 WO1996025513A1 PCT/FR1996/000257 FR9600257W WO9625513A1 WO 1996025513 A1 WO1996025513 A1 WO 1996025513A1 FR 9600257 W FR9600257 W FR 9600257W WO 9625513 A1 WO9625513 A1 WO 9625513A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- yeast cells
- molecules
- type
- biochemical sensor
- biochemical
- Prior art date
Links
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 22
- 150000005829 chemical entities Chemical class 0.000 claims abstract description 6
- 239000000758 substrate Substances 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 238000010353 genetic engineering Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 3
- 238000002703 mutagenesis Methods 0.000 claims description 3
- 231100000350 mutagenesis Toxicity 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 230000010287 polarization Effects 0.000 claims description 2
- 230000004044 response Effects 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 3
- 239000002304 perfume Substances 0.000 abstract description 2
- 239000003344 environmental pollutant Substances 0.000 abstract 1
- 231100000719 pollutant Toxicity 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 102000027257 transmembrane receptors Human genes 0.000 description 6
- 108091008578 transmembrane receptors Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 239000012429 reaction media Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000011278 mitosis Effects 0.000 description 2
- YGYAWVDWMABLBF-UHFFFAOYSA-N Phosgene Chemical compound ClC(Cl)=O YGYAWVDWMABLBF-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000004081 narcotic agent Substances 0.000 description 1
- 238000000424 optical density measurement Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/973—Simultaneous determination of more than one analyte
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/809—Multifield plates or multicontainer arrays
Definitions
- the field of the invention is that of molecular sensors, capable of specifically recognizing molecules and detecting very low contents.
- This type of sensor is particularly interesting in fields as varied as the detection and identification of combat gas, various pollution, narcotics, perfume ..., as soon as we look for the presence of molecules specific whether simple architecture or particularly sophisticated.
- sensors are mainly the ability to recognize a type of molecule and the possibility of amplifying this molecular recognition so as to detect sufficient information to be able to be exploited.
- sensors already exist which operate on the use of antibodies (for specific recognition) and of enzymatic amplification processes for the transmission of information.
- certain tests for detecting molecules operate according to the following principle: A specific antibody (AC-j) of the molecule to be detected is fixed on a membrane; the whole is immersed in a solution containing molecules to be detected (J), allowing the pairing of the antibody (AC-
- - (J) assembly can be immersed in another solution containing another specific anti-J antibody AC2 which can itself be attached to an enzyme.
- the structure described in FIG. 1 is then obtained.
- This molecular grouping can, via the enzyme, transform an entity S into product P at a speed dependent on the catalytic power of the enzyme.
- the enzymatic amplification thus obtained makes it possible to produce a detectable amount of product P even when the molecules to be captured are in very low concentrations. Typically, acidity, basicity, color or any other physico-chemical phenomenon can appear.
- a scheme consists in using an interface between the external medium and a reaction medium connected to a detector, this interface comprising different capture sites targeted molecules and the enzymatic amplification being carried out within the reaction medium.
- the different capture sites must be able to trigger the enzymatic amplification process as soon as they have captured the molecules that we are trying to detect. They must therefore fulfill the following two functions: selectively capture a type of molecules and transmit the information from this capture to a reaction medium which generates by enzymatic amplification, a sufficient signal to the detector (transducer of chemical information into information physical).
- transmembrane receptors integrated within a membrane made up of phospholipids. These transmembrane receptors are made up of transverse elements (type proteins organized in a helix) linked together by molecular bridges, as illustrated in FIGS. 2a and 2b. This set creates on each side of the membrane a reception site, the steric form of which depends on the molecular bridges and on the relative position of the transverse elements allowing, on the external medium side, the capture of a type of molecule (M) for which the transmembrane receptor is adapted, and transmitting the deformation associated with capture, in the reaction medium.
- M type of molecule
- is able to trigger within the cellular medium, a whole process of reactions via enzymes in particular, present in the medium. More specifically, it is known that certain cells see their reproduction process by mitosis (indirect division of cells, into identical cells) disturbed during the capture of certain messenger molecules. Thus when capturing molecules via transmembrane receptors, it is possible to modify the mechanisms intracellular so as to make certain proteins act at the level of genes active in matters of cell reproduction.
- yeast cells appear to be very good candidates for producing biochemical sensors because of their low cost and their robustness compared to other living cells whose maintenance in functional state is problematic and especially thanks to the current knowledge of their genetic heritage.
- the subject of the invention is a biochemical sensor based on yeast cells, the genetic heritage of which has been modified so that said yeast cells are able to pick up a certain type of molecule and at the end of this capture generates detectable information.
- the invention proposes a biochemical sensor characterized in that it comprises:
- a substrate on which yeast cells are deposited capable of capturing a type of messenger (M) and of producing, after this capture, a chemical entity (P);
- the chemical entity produced can advantageously be an optically recognizable protein.
- the detection means can be of the optical density measurement, fluorescence measurement, light polarization measurement, etc. type.
- the substrate is a nourishing medium allowing the reproduction of yeast cells.
- It can advantageously be an anhydrous gel loaded with minerals, sugar etc., on which the yeast cells are deposited, activated subsequently by humidification.
- FIG. 1 illustrates a reaction scheme used in a screening test for molecules in solution, according to known art
- FIG. 2 shows schematically the principle of capture sites existing in living microorganisms; * Figure 2a shows schematically a sectional view of transmembrane receiver;
- FIG. 2b shows schematically a top view of a transmembrane receiver
- the biochemical sensor according to the invention comprises a sensitive detection layer produced from the deposit of cells whose genetic heritage has been modified. These genetic modifications can be carried out by mutagenesis and / or by genetic engineering techniques, followed by a selection from cells obtained from cells sensitive to a given chemical compound (or to a range of compounds).
- mutagenesis is a natural process that involves the random modification of certain genes. By heating, in particular, this natural process can be accelerated over a very large number of times in order to cause natural genetic mutations in order to obtain new transmembrane receptors adapted to the capture of targeted molecules.
- the yeast cells used in the biochemical sensor according to the invention have not only been deceived in order to make them sensitive to different types of molecules, but they have also been so to make them capable of producing an entity. particular detectable, attesting to the capture of the targeted molecules.
- the genome, the whole genetic heritage, of yeast cells is better and better controlled, it thus becomes possible by the identification of genes and their functions to come by genetic manipulation, to disturb this heritage in order to change some features. So we can come and insert certain elements into this genetic heritage, making it possible to adapt transmembrane receptors to different types of molecules.
- the capture of a given type of molecule can in turn trigger a whole chain of specific chemical reactions leading to the specific appearance of a protein. especially.
- This protein can be selected for its optical properties.
- yeast cells are isolated, the genetic manipulation of which has led to making the yeast cell sensitive to molecules other than those which are naturally capable of being captured and has also led to the entity production
- This experiment can be carried out and adapted to different types of molecules (M) so as to have a series of sensors based on yeast cells responding specifically to molecules (Mi) with identical or non-entity production (P) to detect.
- the sensor according to the invention can more generally identify a whole series of molecules (M) when it results from the association of N elementary biological sensors designed to recognize each a specific type of molecules (Mi).
- a mosaic of N elementary sensors can be produced, capable of producing the same colored protein when they capture a molecule (Mi).
- Mi molecule
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/722,018 US5834218A (en) | 1995-02-17 | 1996-02-16 | Biochemical sensor |
JP8524725A JPH09512179A (ja) | 1995-02-17 | 1996-02-16 | 生化学的センサ |
EP96904154A EP0756635A1 (fr) | 1995-02-17 | 1996-02-16 | Capteur biochimique |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9501829A FR2730812B1 (fr) | 1995-02-17 | 1995-02-17 | Capteur biochimique |
FR95/01829 | 1995-02-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1996025513A1 true WO1996025513A1 (fr) | 1996-08-22 |
Family
ID=9476240
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR1996/000257 WO1996025513A1 (fr) | 1995-02-17 | 1996-02-16 | Capteur biochimique |
Country Status (5)
Country | Link |
---|---|
US (1) | US5834218A (fr) |
EP (1) | EP0756635A1 (fr) |
JP (1) | JPH09512179A (fr) |
FR (1) | FR2730812B1 (fr) |
WO (1) | WO1996025513A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6117643A (en) * | 1997-11-25 | 2000-09-12 | Ut Battelle, Llc | Bioluminescent bioreporter integrated circuit |
US7141414B2 (en) * | 2002-09-16 | 2006-11-28 | Hewlett-Packard Development Company, L.P. | Biosensor |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD212052A1 (de) * | 1982-10-04 | 1984-08-01 | Inst F Tech Mikrobiologie | Anwendung traegerfixierter mikroorganismen oder organellen fuer sensoren |
DE4301087A1 (de) * | 1993-01-16 | 1994-07-21 | Lange Gmbh Dr Bruno | Vorrichtung zur Bestimmung des biochemischen Sauerstoffbedarfs |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1424482A (fr) * | 1964-12-01 | 1966-01-14 | Csf | élément de circuit électrique intégré à réactance inductive |
CA1237645A (fr) * | 1982-12-21 | 1988-06-07 | John H. Fisher | Technique analytique |
US4935345A (en) * | 1987-04-07 | 1990-06-19 | Arizona Board Of Regents | Implantable microelectronic biochemical sensor incorporating thin film thermopile |
IL98150A0 (en) * | 1990-05-17 | 1992-08-18 | Adeza Biomedical Corp | Highly reflective biogratings and method for theirhighly reflective biogratings and method production |
FR2663466A1 (fr) * | 1990-06-15 | 1991-12-20 | Thomson Csf | Composant semiconducteur a jonction schottky pour amplification hyperfrequence et circuits logiques rapides, et procede de realisation d'un tel composant. |
-
1995
- 1995-02-17 FR FR9501829A patent/FR2730812B1/fr not_active Expired - Fee Related
-
1996
- 1996-02-16 EP EP96904154A patent/EP0756635A1/fr not_active Withdrawn
- 1996-02-16 JP JP8524725A patent/JPH09512179A/ja active Pending
- 1996-02-16 WO PCT/FR1996/000257 patent/WO1996025513A1/fr not_active Application Discontinuation
- 1996-02-16 US US08/722,018 patent/US5834218A/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DD212052A1 (de) * | 1982-10-04 | 1984-08-01 | Inst F Tech Mikrobiologie | Anwendung traegerfixierter mikroorganismen oder organellen fuer sensoren |
DE4301087A1 (de) * | 1993-01-16 | 1994-07-21 | Lange Gmbh Dr Bruno | Vorrichtung zur Bestimmung des biochemischen Sauerstoffbedarfs |
Non-Patent Citations (3)
Title |
---|
DATABASE CAPLUS [online] XP002004837, accession no. STN Database accession no. 1992:507511 * |
J. CHEN ET AL: "An amperometric alcohol based on chemically permeabilized methylotrophic microorganisms.", BIOTECHNOL. PROG., vol. 8, no. 2, 1992, pages 161 - 164, XP002004836 * |
KORPIN YA. I. ET AL.: "CELLULAR MICROBIOSENSORS FOR METHANOL AND ETHANOL DETERMINATION ON THE BASIS OF PH-SENSITIVE FIELD EFFECT TRANSISTORS", UKR. BIOKHIM. ZH., vol. 64, no. 3, 1992, pages 96 - 100 * |
Also Published As
Publication number | Publication date |
---|---|
US5834218A (en) | 1998-11-10 |
FR2730812B1 (fr) | 1997-03-14 |
EP0756635A1 (fr) | 1997-02-05 |
FR2730812A1 (fr) | 1996-08-23 |
JPH09512179A (ja) | 1997-12-09 |
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