WO1996017068A2 - Vegetaux resistant aux agents pathogenes et leur procede de production - Google Patents

Vegetaux resistant aux agents pathogenes et leur procede de production Download PDF

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Publication number
WO1996017068A2
WO1996017068A2 PCT/DE1995/001698 DE9501698W WO9617068A2 WO 1996017068 A2 WO1996017068 A2 WO 1996017068A2 DE 9501698 W DE9501698 W DE 9501698W WO 9617068 A2 WO9617068 A2 WO 9617068A2
Authority
WO
WIPO (PCT)
Prior art keywords
plants
vip
proteins
cdna
plant
Prior art date
Application number
PCT/DE1995/001698
Other languages
German (de)
English (en)
Other versions
WO1996017068A3 (fr
Inventor
Uwe Sonnewald
Karin Herbers
Original Assignee
Institut für Pflanzengenetik und Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut für Pflanzengenetik und Kulturpflanzenforschung filed Critical Institut für Pflanzengenetik und Kulturpflanzenforschung
Publication of WO1996017068A2 publication Critical patent/WO1996017068A2/fr
Publication of WO1996017068A3 publication Critical patent/WO1996017068A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne l'isolation de nouveaux clones d'ADNc induits par agents pathogènes, ainsi qu'un procédé permettant de produire des végétaux résistant aux agents pathogènes, selon lequel un produit de recombinaison génique chimérique comprenant un promoteur actif, une région codant les protéines induites par virus (protéines VIP) et un signal de polyadénylation, est introduit dans le patrimoine héréditaire des végétaux. Les nouveaux gènes de protéine VIP ont été isolés à partir de pieds de tabac transgéniques qui présentent une résistance élevée aux virus. Les protéines VIP constituent une nouvelle catégorie de protéines PR. L'invention concerne en outre de nouvelles séquences d'ADN qui codent les protéines VIP, ainsi que des végétaux et des parties de végétaux dont le patrimoine héréditaire se trouve modifié par ledit procédé. Ces nouveaux végétaux ont une résistance élevée aux agents pathogènes. L'invention s'applique à l'agriculture et au génie génétique.
PCT/DE1995/001698 1994-11-26 1995-11-21 Vegetaux resistant aux agents pathogenes et leur procede de production WO1996017068A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944442179 DE4442179C2 (de) 1994-11-26 1994-11-26 Pathogenresistente Pflanzen und Verfahren zu ihrer Herstellung
DEP4442179.6 1994-11-26

Publications (2)

Publication Number Publication Date
WO1996017068A2 true WO1996017068A2 (fr) 1996-06-06
WO1996017068A3 WO1996017068A3 (fr) 1996-08-29

Family

ID=6534259

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1995/001698 WO1996017068A2 (fr) 1994-11-26 1995-11-21 Vegetaux resistant aux agents pathogenes et leur procede de production

Country Status (2)

Country Link
DE (1) DE4442179C2 (fr)
WO (1) WO1996017068A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (fr) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Plantes transgéniques résistantes aux maladies
EP0460753A2 (fr) * 1990-06-07 1991-12-11 Mogen International N.V. Nouvelles préparations antifungiques, procédé pour leur production, procédé d'obtention des plantes ayant une sensibilité réduite aux champignons
WO1994016077A1 (fr) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Procede pour induire une resistance aux maladies dans des plantes
WO1994024295A1 (fr) * 1993-04-12 1994-10-27 Ciba-Geigy Ag Regulation exogene de l'expression des genes chez des plantes
WO1995019443A2 (fr) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Sequences d'adn anti-pathogenes et a regulation chimique, et leurs utilisations

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4003045A1 (de) * 1990-02-02 1991-08-08 Hoechst Ag Virus/herbizidresistenz-gene, verfahren zu ihrer herstellung und ihre verwendung
EP0479180A3 (en) * 1990-10-05 1992-08-26 Hoechst Aktiengesellschaft Virus resistant plants, method for their production
EP0480310A3 (en) * 1990-10-09 1992-11-19 Takeda Chemical Industries, Ltd. Virus-resistant tomato plants
DE4040954C2 (de) * 1990-12-20 2001-05-17 Max Planck Gesellschaft Verfahren zur Erzeugung von pathogen-resistenten Pflanzen
DE69233410D1 (de) * 1991-10-04 2004-10-21 Univ North Carolina State Pathogenresistente transgene pflanzen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (fr) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Plantes transgéniques résistantes aux maladies
EP0460753A2 (fr) * 1990-06-07 1991-12-11 Mogen International N.V. Nouvelles préparations antifungiques, procédé pour leur production, procédé d'obtention des plantes ayant une sensibilité réduite aux champignons
WO1994016077A1 (fr) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Procede pour induire une resistance aux maladies dans des plantes
WO1994024295A1 (fr) * 1993-04-12 1994-10-27 Ciba-Geigy Ag Regulation exogene de l'expression des genes chez des plantes
WO1995019443A2 (fr) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Sequences d'adn anti-pathogenes et a regulation chimique, et leurs utilisations

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CURRENT PLANT SCIENCE AND BIOTECHNOLOGY IN AGRICULTURE, VOL. 14. ADVANCES IN MOLECULAR GENETICS OF PLANT-MICROBE INTERACTIONS, VOL. 2. 6TH INTERNATIONAL SYMPOSIUM ON MOLECULAR PLANT-MICROBE INTERACTIONS, SEATTLE, WASHINGTON, USA, JULY 1992., 1993, Seiten 527-533, XP002003881 ALEXANDER, D., ET AL.: "Systemic acquired resistance in tobacco: use of transgenic expression to study the functions of pathogenesis-related proteins" *
EMBL SEQUENCE DATABASE, RELEASE 44, 19-JUN-1995, ACCESSION NO. X83851., XP002003883 HERBERS, K., ET AL.: "N.tabacum mRNA for PAR-1b" *
EMBL SEQUENCE DATABASE, RELEASE 44, 19-JUN-1995, XP002003884 HEBERS, K., ET AL.: "N.tabacum mRNA for PAR-1c" *
EMBL SEQUENCE DATABASE, RELEASE 44, 19-JUN-1995, XP002003885 HERBERS, K., ET AL.: "N.tabacum mRNA for PAR-1a" *
FRITIG, B. AND M. LEGRAND (ED.). DEVELOPMENTS IN PLANT PATHOLOGY, VOL. 2. MECHANISMS OF PLANT DEFENSE RESPONSES. 2ND INTERNATIONAL CONFERENCE OF THE EUROPEAN FOUNDATION FOR PLANT PATHOLOGY, STRASBOURG, FRANCE, AUGUST 24-27, 1992. KLUWER ACADEMIC , 1993, XP002003880 LAWTON, K., ET AL.: "The molecular biology of systemic acquired resistance" *
PHOTOSYNTHETICA (PRAGUE) 30 (4). 1994. 575-582. , XP000566783 BADUR R., ET AL.: "Induction of pathogenesis-related proteins in sugar accumulating tobacco leaves." *
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, Bd. 90, August 1993, WASHINGTON US, Seiten 7327-7331, XP002003882 ALEXANDER, D., ET AL.: "Increased toleranceto two oomycte pathogens in transgenic tobacco expressing pathogenesis-related protein 1a" *

Also Published As

Publication number Publication date
DE4442179C2 (de) 1996-12-12
WO1996017068A3 (fr) 1996-08-29
DE4442179A1 (de) 1996-05-30

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