WO1996016334A3 - Screening technique - Google Patents

Screening technique Download PDF

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Publication number
WO1996016334A3
WO1996016334A3 PCT/GB1995/002732 GB9502732W WO9616334A3 WO 1996016334 A3 WO1996016334 A3 WO 1996016334A3 GB 9502732 W GB9502732 W GB 9502732W WO 9616334 A3 WO9616334 A3 WO 9616334A3
Authority
WO
WIPO (PCT)
Prior art keywords
tumour cells
inhibitor
cells
sample
guanidinobenzoatase
Prior art date
Application number
PCT/GB1995/002732
Other languages
French (fr)
Other versions
WO1996016334A2 (en
Inventor
Frank Steven
Original Assignee
Univ Manchester
Frank Steven
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9423617A external-priority patent/GB9423617D0/en
Priority claimed from GB9423616A external-priority patent/GB9423616D0/en
Priority claimed from GB9423615A external-priority patent/GB9423615D0/en
Application filed by Univ Manchester, Frank Steven filed Critical Univ Manchester
Priority to AU38784/95A priority Critical patent/AU3878495A/en
Publication of WO1996016334A2 publication Critical patent/WO1996016334A2/en
Publication of WO1996016334A3 publication Critical patent/WO1996016334A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oncology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Hospice & Palliative Care (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method of detecting tumour cells in a cell sample from a human or animal comprises treating the sample with a first competitive inhibitor which binds to isoenzymic forms of guanidinobenzoatase on the surfaces of tumour cells and other cells having distinct isoenzymic forms of guanidinobenzoatase. Subsequently the first inhibitor is selectively displacing from either the tumour cells or said other cells by a second competitive inhibitor which is distinguishable from the first inhibitor whereby the presence of tumour cells in the sample may be detected. For preference, it is the first inhibitor which is displaced from the tumour cells and the second inhibitor is fluorescent. Non-tumour cells do not fluoresce and the tumour cells are easily distingued by their fluorescence.
PCT/GB1995/002732 1994-11-23 1995-11-23 Screening technique WO1996016334A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU38784/95A AU3878495A (en) 1994-11-23 1995-11-23 Screening technique

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB9423615.5 1994-11-23
GB9423616.3 1994-11-23
GB9423617.1 1994-11-23
GB9423617A GB9423617D0 (en) 1994-11-23 1994-11-23 Preparation of cell samples
GB9423616A GB9423616D0 (en) 1994-11-23 1994-11-23 Screening technique
GB9423615A GB9423615D0 (en) 1994-11-23 1994-11-23 Screening technique

Publications (2)

Publication Number Publication Date
WO1996016334A2 WO1996016334A2 (en) 1996-05-30
WO1996016334A3 true WO1996016334A3 (en) 1996-08-01

Family

ID=27267483

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1995/002732 WO1996016334A2 (en) 1994-11-23 1995-11-23 Screening technique

Country Status (2)

Country Link
AU (1) AU3878495A (en)
WO (1) WO1996016334A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2553637T3 (en) * 2006-03-06 2015-12-10 Zetiq Technologies Ltd. Methods to identify a cellular phenotype
US9182403B2 (en) 2009-05-19 2015-11-10 Zetiq Technologies Ltd. Kits for and methods of differential staining of cervical cancer cells and/or tissues

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ANTICANCER RES., vol. 11, no. 1, pages 143 - 150 *
ANTICANCER RES., vol. 8, no. 6, pages 1179 - 1183 *
BIOCHEM. SOC. TRANS., vol. 15, no. 6, pages 1109 *
BIOL. CHEM. HOPPE-SEYLER, vol. 369(suppl.), pages 137 - 143 *
CHEMICAL ABSTRACTS, vol. 107, no. 15, 12 October 1987, Columbus, Ohio, US; abstract no. 131847, F. S. STEVEN ET AL.: "Evidence for in vivo inhibition of a cell surface protease associated with tumor cells." page 517; column 2; *
CHEMICAL ABSTRACTS, vol. 108, no. 15, 11 April 1988, Columbus, Ohio, US; abstract no. 127378, F. S. STEVEN ET AL.: "Inhibitors of guanidinobenzoatase: evidence for multiple forms of this protease on different tumor cells." page 341; column 1; *
CHEMICAL ABSTRACTS, vol. 109, no. 9, 29 August 1988, Columbus, Ohio, US; abstract no. 70869, F. S. STEVEN ET AL.: "Inhibitors of guanidinobenzoatase and their possible role in cell migration." page 458; column 1; *
CHEMICAL ABSTRACTS, vol. 110, no. 25, 19 June 1989, Columbus, Ohio, US; abstract no. 225086, F. STEVEN ET AL.: "A fluorescent study of ligands for guanidinobenzoatase, a protease associated with tumor cells." page 23; column 2; *
CHEMICAL ABSTRACTS, vol. 115, no. 3, 22 July 1991, Columbus, Ohio, US; abstract no. 26918, F. S. STEVEN ET AL.: "Evidence for distinct isoenzymic forms of a cell surface protease on normal colonic cells and on carcinoma cells from the same colon." page 550; column 1; *
CHEMICAL ABSTRACTS, vol. 115, no. 5, 5 August 1991, Columbus, Ohio, US; abstract no. 44714, F. S. STEVEN ET AL.: "Temporary competitive inhibition of a tumor cell surface protease as a protective mechanism in the preparation of the membrane bound native enzyme in the presence of excess cytoplasmic inhibitors." page 367; column 2; *
CHEMICAL ABSTRACTS, vol. 120, no. 3, 17 January 1994, Columbus, Ohio, US; abstract no. 26195, F. S. STEVEN ET AL.: "Affinity preparation of a protein inhibitor recognizing a cell surface protease" page 368; column 1; *
J. ENZYME INHIB., vol. 2, no. 2, pages 117 - 127 *
J. ENZYME INHIB., vol. 5, no. 1, pages 77 - 85 *
J. ENZYME INHIB., vol. 7, no. 1, pages 65 - 76 *

Also Published As

Publication number Publication date
AU3878495A (en) 1996-06-17
WO1996016334A2 (en) 1996-05-30

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