WO1996012397A3 - Recombinant antibody capable of binding to pna/nucleic acid complexes - Google Patents

Recombinant antibody capable of binding to pna/nucleic acid complexes Download PDF

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Publication number
WO1996012397A3
WO1996012397A3 PCT/DK1995/000486 DK9500486W WO9612397A3 WO 1996012397 A3 WO1996012397 A3 WO 1996012397A3 DK 9500486 W DK9500486 W DK 9500486W WO 9612397 A3 WO9612397 A3 WO 9612397A3
Authority
WO
WIPO (PCT)
Prior art keywords
pna
pnas
nucleic acids
nucleic acid
complexes
Prior art date
Application number
PCT/DK1995/000486
Other languages
French (fr)
Other versions
WO1996012397A2 (en
Inventor
Jens Joergen Hyldig-Nielsen
Karl-Johan Pluzek
Original Assignee
Dako As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dako As filed Critical Dako As
Priority to JP8513602A priority Critical patent/JPH11511642A/en
Priority to AU43272/96A priority patent/AU4327296A/en
Priority to EP95942056A priority patent/EP0837884A2/en
Publication of WO1996012397A2 publication Critical patent/WO1996012397A2/en
Publication of WO1996012397A3 publication Critical patent/WO1996012397A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Recombinant antibodies or fragments thereof that are capable of binding to complexes formed between PNA (Peptide Nucleic Acid) and nucleic acids, particularly to PNA/DNA or PNA/RNA complexes, are described. The preferred antibodies are recombinant antibodies that bind to PNA/DNA OR PNA/RNA complexes, but not to single-stranded PNAs, double-stranded nucleic acids or single-stranded nucleic acids. PNAs are newly developed, not naturally occurring compounds of which some have a polyamide backbone bearing a plurality of ligands such as naturally occurring nucleobases attached to the backbone through a suitable linker. Some PNAs have been shown to possess a surprising high affinity for complementary nucleic acid, forming very stable and specific complexes. Such PNAs are thus suitable as hybridization probes for detection of nucleic acids. The antibodies now provided render the PNAs very usable as hybridization probes. The antibodies provided are useful in the capture, recognition, detection, identification or quantitation of nucleic acids in biological samples, via their ability to react with PNA-nucleic acid complexes.
PCT/DK1995/000486 1995-06-22 1995-12-01 Recombinant antibody capable of binding to pna/nucleic acid complexes WO1996012397A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP8513602A JPH11511642A (en) 1995-06-22 1995-12-01 Recombinant antibody capable of binding to PNA / nucleic acid complex
AU43272/96A AU4327296A (en) 1995-06-22 1995-12-01 Recombinant antibody capable of binding to pna/nucleic acid complexes
EP95942056A EP0837884A2 (en) 1995-06-22 1995-12-01 Recombinant antibody capable of binding to pna/nucleic acid complexes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK0717/95 1995-06-22
DK71795 1995-06-22

Publications (2)

Publication Number Publication Date
WO1996012397A2 WO1996012397A2 (en) 1996-05-02
WO1996012397A3 true WO1996012397A3 (en) 1996-06-20

Family

ID=8096736

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1995/000486 WO1996012397A2 (en) 1995-06-22 1995-12-01 Recombinant antibody capable of binding to pna/nucleic acid complexes

Country Status (4)

Country Link
EP (1) EP0837884A2 (en)
JP (1) JPH11511642A (en)
AU (1) AU4327296A (en)
WO (1) WO1996012397A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
US6756207B1 (en) * 1997-02-27 2004-06-29 Cellomics, Inc. System for cell-based screening
US6287772B1 (en) 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0135159A2 (en) * 1983-08-19 1985-03-27 Cetus Corporation Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same
WO1992020703A1 (en) * 1991-05-24 1992-11-26 Ole Buchardt The use of nucleic acid analogues in diagnostics and analytical procedures
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
WO1995017430A1 (en) * 1993-12-23 1995-06-29 Dako A/S Polyclonal antibody to pna/nucleic acid complexes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0135159A2 (en) * 1983-08-19 1985-03-27 Cetus Corporation Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same
WO1992020703A1 (en) * 1991-05-24 1992-11-26 Ole Buchardt The use of nucleic acid analogues in diagnostics and analytical procedures
WO1995015974A1 (en) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Protecting nucleic acids and methods of analysis
WO1995017430A1 (en) * 1993-12-23 1995-06-29 Dako A/S Polyclonal antibody to pna/nucleic acid complexes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
H. ÖRUM ET AL.: "Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage.", NUCLEIC ACIDS RESEARCH, vol. 21, no. 19, 25 September 1993 (1993-09-25), OXFORD, GB, pages 4491 - 4498, XP002393352 *
M. EGHOLM ET AL.: "Peptide nucleic acids containing adenine or guanine recognize thymine and cytosine in complementary DNA sequences.", JOURNAL OF THE CHEMICAL SOCIETY. CHEMICAL COMMUNICATIONS, no. 9, CAMBRIDGE, GB, pages 800 - 801 *
M. EGHOLM ET AL.: "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules.", NATURE, vol. 365, no. 6446, 7 October 1993 (1993-10-07), LONDON, GB, pages 566 - 568, XP000606272, DOI: doi:10.1038/365566a0 *
O. BUCHARDT ET AL.: "Peptide nucleic acids and their potential applications in biotechnology.", TRENDS IN BIOTECHNOLOGY, vol. 11, no. 9, CAMBRIDGE, GB, pages 384 - 386, XP023595026, DOI: doi:10.1016/0167-7799(93)90097-S *
P. WITTUNG ET AL.: "DNA-like double helix formed by peptide nucleic acid.", NATURE, vol. 368, no. 6471, 7 April 1994 (1994-04-07), LONDON, GB, pages 561 - 563 *

Also Published As

Publication number Publication date
EP0837884A2 (en) 1998-04-29
AU4327296A (en) 1996-05-15
JPH11511642A (en) 1999-10-12
WO1996012397A2 (en) 1996-05-02

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