WO1995027065A1 - Method for selecting transformed eucaryotic cells and cells obtained thereby - Google Patents

Method for selecting transformed eucaryotic cells and cells obtained thereby Download PDF

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Publication number
WO1995027065A1
WO1995027065A1 PCT/FR1995/000425 FR9500425W WO9527065A1 WO 1995027065 A1 WO1995027065 A1 WO 1995027065A1 FR 9500425 W FR9500425 W FR 9500425W WO 9527065 A1 WO9527065 A1 WO 9527065A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
streptothricin
dna
sat
selecting transformed
Prior art date
Application number
PCT/FR1995/000425
Other languages
French (fr)
Inventor
Jean Brevet
Joanna Jelenska
Jacques Tempe
Erhard Tietze
Original Assignee
Lvmh Recherche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lvmh Recherche filed Critical Lvmh Recherche
Priority to EP95916702A priority Critical patent/EP0754233A1/en
Priority to AU23747/95A priority patent/AU2374795A/en
Priority to NZ284494A priority patent/NZ284494A/en
Priority to JP7525468A priority patent/JPH09510876A/en
Publication of WO1995027065A1 publication Critical patent/WO1995027065A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Abstract

Method for producing animal or plant eucaryotic cells or cell clusters, resistant to streptothricin or analogues thereof, including the steps of transforming said eucaryotic cells with a plasmid containing an exogenous DNA sequence coding for a streptothricin acetyl transferase (SAT), which comprises the DNA sequence coding for said streptothricin acetyl transferase (DNA-SAT) in an appropriate position, and one or more DNA sequences coding for elements controlling the expression of said DNA-SAT sequence in said cells, and selecting the cells resistant to streptothricin or its analogues after their transformation.
PCT/FR1995/000425 1994-04-05 1995-04-04 Method for selecting transformed eucaryotic cells and cells obtained thereby WO1995027065A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP95916702A EP0754233A1 (en) 1994-04-05 1995-04-04 Method for selecting transformed eucaryotic cells and cells obtained thereby
AU23747/95A AU2374795A (en) 1994-04-05 1995-04-04 Method for selecting transformed eucaryotic cells and cells obtained thereby
NZ284494A NZ284494A (en) 1994-04-05 1995-04-04 Transformed cells with streptothricin resistance
JP7525468A JPH09510876A (en) 1994-04-05 1995-04-04 Method for selecting transformed eukaryotic cells and cells obtained using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9403969A FR2718152B1 (en) 1994-04-05 1994-04-05 Method for selecting transformed eukaryotic cells and cells obtained.
FR94/03969 1994-04-05

Publications (1)

Publication Number Publication Date
WO1995027065A1 true WO1995027065A1 (en) 1995-10-12

Family

ID=9461737

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1995/000425 WO1995027065A1 (en) 1994-04-05 1995-04-04 Method for selecting transformed eucaryotic cells and cells obtained thereby

Country Status (9)

Country Link
EP (1) EP0754233A1 (en)
JP (1) JPH09510876A (en)
AU (1) AU2374795A (en)
CA (1) CA2187170A1 (en)
FR (1) FR2718152B1 (en)
IL (1) IL113224A0 (en)
NZ (1) NZ284494A (en)
WO (1) WO1995027065A1 (en)
ZA (1) ZA952778B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001061019A2 (en) * 2000-02-18 2001-08-23 Mcgill University A dominant selectable marker for gene transformation and disruption in yeasts

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD285374A5 (en) * 1989-06-29 1990-12-12 Akad Wissenschaften Ddr METHOD FOR PRODUCING A PROKARYOTIC VECTOR PLASMIDE
US5073675A (en) * 1989-05-26 1991-12-17 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5073675A (en) * 1989-05-26 1991-12-17 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
DD285374A5 (en) * 1989-06-29 1990-12-12 Akad Wissenschaften Ddr METHOD FOR PRODUCING A PROKARYOTIC VECTOR PLASMIDE

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 109, no. 13, Columbus, Ohio, US; abstract no. 105615, TIETZE, E., ET AL.: "Cloning and preliminary characterization of the streptothricin resistance determinants of the transposons Tn1825 and 1826" *
CLAES, B., ET AL.: "Organ-dependent regulation of a plant promoter isolated from rice by 'promoter-trapping' in tobacco", THE PLANT JOURNAL, vol. 1, no. 1, pages 15 - 26 *
EMBL SEQUENCE ACC. NO Z48231. REL.42. 13-2-1995. TIETZE, ET AL. E.coli IncQ plasmid pIE639 sat3 gene and aphA gene. *
GOLD, S.E., ET AL.: "Three selectable markers for transformation of Ustilago maydis", GENE, vol. 142, 16 May 1994 (1994-05-16), pages 225 - 230 *
HEIM, U., ET AL.: "Nucleotide sequence of a plasmid born streptothricin-acetyl-transferase gene (sat-1)", NUCLEIC ACIDS RESEARCH, vol. 17, no. 17, ARLINGTON, VIRGINIA US, pages 7103 *
J. BASIC MICROBIOL., vol. 28, no. 1-2, pages 129 - 136 *
KIDA, T., ET AL.: "Isolation of 2 streptothricin-like antibiotics. No. 6241-A and No. 6421-B as inhibitors of de novo starch synthesis and their herbicidal activity", AGRIC. BIOL. CHEM., vol. 49, no. 6, pages 1839 - 1844 *
TIETZE, E., ET AL.: "Characterization of new R plasmids beloning to the incompatibility group IncQ", JOURNAL OF BASIC MICROBIOLOGY, vol. 29, pages 695 - 706 *
TIETZE, E., ET AL.: "DNA probes for studying streptothricin resistance evolution in enteric bacteria", JOURNAL OF BASIC MICROBIOLOGY, vol. 30, no. 4, pages 279 - 287 *
TIETZE, E., ET AL.: "Nucleotide sequence of the streptothricin-acetyl-transferase gene sat-2", NUCLEIC ACIDS RESEARCH, vol. 18, no. 5, ARLINGTON, VIRGINIA US, pages 1283 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001061019A2 (en) * 2000-02-18 2001-08-23 Mcgill University A dominant selectable marker for gene transformation and disruption in yeasts
WO2001061019A3 (en) * 2000-02-18 2002-09-26 Univ Mcgill A dominant selectable marker for gene transformation and disruption in yeasts

Also Published As

Publication number Publication date
JPH09510876A (en) 1997-11-04
IL113224A0 (en) 1995-07-31
EP0754233A1 (en) 1997-01-22
CA2187170A1 (en) 1995-10-12
ZA952778B (en) 1996-01-05
FR2718152B1 (en) 1996-06-28
FR2718152A1 (en) 1995-10-06
NZ284494A (en) 1998-09-24
AU2374795A (en) 1995-10-23

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