WO1995019424A1 - Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques - Google Patents

Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques Download PDF

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Publication number
WO1995019424A1
WO1995019424A1 PCT/US1995/000019 US9500019W WO9519424A1 WO 1995019424 A1 WO1995019424 A1 WO 1995019424A1 US 9500019 W US9500019 W US 9500019W WO 9519424 A1 WO9519424 A1 WO 9519424A1
Authority
WO
WIPO (PCT)
Prior art keywords
envelope
bioreactor
housing
algae
fluid
Prior art date
Application number
PCT/US1995/000019
Other languages
English (en)
Inventor
Amnon Yogev
Dan Yakir
Original Assignee
Yeda Research And Development Co. Ltd.
Rycus, Avigail
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Research And Development Co. Ltd., Rycus, Avigail filed Critical Yeda Research And Development Co. Ltd.
Priority to AU15969/95A priority Critical patent/AU681243B2/en
Priority to US08/669,320 priority patent/US5958761A/en
Priority to JP7519032A priority patent/JPH09507390A/ja
Publication of WO1995019424A1 publication Critical patent/WO1995019424A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C12M41/08—Means for changing the orientation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C12M41/10—Filtering the incident radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

Definitions

  • the present invention relates to a bioreactor for improved productivity of photosynthetic algae. More particularly, the invention is concerned with bioreactors and bioreactor systems for optical enhancement of photosynthetic productivity of algae.
  • microalgae In the past decade, there has been considerable activity relating to production of photosynthetic microalgae for commercial purposes. Special industries aiming to produce health food, food additives, animal feed, biofertilizers and an assortment of natural products (most notably ⁇ -carotene) have been established. Recently, microalgae have been suggested as a means to sequester carbon from the industry, and hydrogen producing algae as a source of energy.
  • EET RULE26 devices and to provide a bioreactor in which the light regime therein is improved.
  • Still a further object of the present invention is to provide a bioreactor in which the spectral quality of the impinging light can be adjusted in accordance with specific requirements of an algae species which is produced.
  • Yet still a further object of the present invention is to provide a bioreactor in which the temperature prevailing inside the reactor can be adjusted and controlled.
  • a bioreactor for improved productivity of photosynthetic algae comprising a tubular housing surrounding a tubular envelope located therein and defining a space therebetween to be filled with fluid, said housing and envelope being made of at least a translucent material, said housing and envelope having inlet and outlet ports providing access to said space and to the interior of said envelope, and means for mixing a algae media disposed inside said envelope.
  • the invention also provides a bioreactor system for improved productivity of photosynthetic algae, comprising a tubular housing surrounding a tubular envelope located therein and defining a space therebetween, said housing and envelope being made of at least a translucent material, said housing and envelope having inlet and outlet ports providing access to said space and the interior of said envelope, and means for mixing a algae media disposed inside said envelope, said envelope containing ' a fluid of selective refractive index, wherein, for a given geometrical relationship between said housing and envelope the radiation concentration power is controlled by modifying the refractive index of said fluid.
  • Fig. 1 is an isometric and schematic illustration of a bioreactor according to the present invention
  • Fig. 2 is a cross-sectional view of a further embodiment of a bioreactor according to the present invention
  • Fig. 3 is a schematic illustration of the bioreactor of
  • Fig. 1 as utilized with a solar concentrator.
  • a bioreactor 2 particularly a bioreactor for improving the productivity of photosynthetic algae by optical means.
  • the bioreactor 2 includes an outer tubular housing 4, and an inner tubular envelope 6.
  • the housing 4 and the envelope 6 are cylindrical and coaxially disposed with respect to each other.
  • Both the housing 4 and the envelope 6 are made of at least a translucent and preferably, of a transparent, material.
  • mixing means 8 e.g., composed of a plurality of vanes 10 mounted on an axis 12 driven by a motor 14.
  • the end portions of the housing 4 and the envelope 6 are connected to common bases or end walls 16, 16' .
  • the outer surface of the envelope 6 and the inner surface of housing 4 define thereinbetween a space 18 to be filled with fluid, the access to which space is effected via an inlet nozzle 20 and an outlet nozzle 22.
  • the two nozzles 20 and 22 are in fluid communication through a heat exchanger/ pump 24.
  • Access to and from the volume inside the envelope 6 is accomplished at one end wall thereof through an opening or a capillary port 26 and at the other end wall through an injector 28.
  • the walls 16, 16' are dismountable to facilitate convenient access to the volume inside the housing and envelope for cleaning and other purposes.
  • the mixing of algae media introduced into the envelope 6 can be effected by other means than the mechanical mixer shown in Fig. 1.
  • the stream of air can also be utilized for mixing the algae media.
  • the air stream can be propelled through a duct replacing the axis 12, which duct is provided with small holes or is made of porous material through which small bubbles are emitted causing a mixing action.
  • mixing can be achieved by a pump (not shown), circulating the algae media via several bioreactors, inter-connected in series.
  • the bioreactor 2 can be scaled up by increasing the diameters of the housing 4 and envelope 6.
  • a rod 30 inside the envelope 6, so as to form an annular space 32 between the surface of the rod and the inside surface of the envelope.
  • the surface of the rod 30 may be coated with material to provide reflection of the impinging radiation.
  • a tube with or without a reflective coating instead of a rod, there may be inserted a tube with or without a reflective coating. If the tube is transparent, there may not be a need for such coating.
  • the more suitable mixing means would be air bubbles or circulating means, as described above.
  • FIG. 3 there is seen the bioreactor 2 located in the center of a Compound Parabolic solar Concentrator (CPC) 34 so as to form a solar bioreactor of a higher degree of efficiency by providing additional solar radiation when required and by the superior distribution of light all around the bioreactor 2.
  • CPC Compound Parabolic solar Concentrator
  • the concentration power is determined by the ratio of the diameters of the housing 4 and envelope 6 (in a case where both the housing and envelope are cylindrical) , as well as by the refractive index of the materials used, which materials should be adjusted with respect to the selected diameters.
  • the external solution poured in the space 18, provides the means for spectral and temperature controls.
  • the spectral quality of the solution can be modified by including therein color filters and/or fluorescence dyes.
  • Two filters, e.g., rodamine or dicromite can be used in the solution, which filters modify the red to blue ratio of the light reaching the algae.
  • the walls of the housing 4 and envelope 6, as well as the solution inside the space 18 also act as light filters, in effect removing substantially all of the UV and IR radiation.
  • the same solution prevailing inside the space 18 is also utilized to control the temperatures of the algae inside the envelope 6 by circulating same through the heat exchanger/pump 24 at a controlled rate.
  • Nutrients are provided by the algae media itself, while C0 2 is supplied thereto by injecting same via the capillary port 26.
  • the carbon supplied is controlled by a PH-stat controller (not shown) operated by a thin electrode inserted into the envelope 6 through port 26.
  • a solar bioreactor 2 was constructed of tubular housing 4 and envelope 6, both made of glass, having respective diameters of 5 and 7.5 cm (ratio of diameters 1.5).
  • the refractive index of the external and internal media was adjusted to 1.5 by dissolving NaCl providing 1.5 times incident sunlight at the inner surface of the envelope (i.e., at the surface of the algae media).
  • the maximal depth of the algae media was about 2.5 cm and it was efficiently mechanically stirred by a series of vanes 10 rotating at a speed of 16 revolutions per minutes, or alternatively, stirred by air bubbles.
  • the bioreactor 2 was placed horizonally in parallel to the sun's daily track, or perpendicularly to the solar orbit.
  • a dilute solution of Dunaliella bardawil algae was used as a model and was put in the envelope 6. Temperature in the external solution was adjusted to provide a constant temperature of 27-32°C. A similar reactor was placed inside a CPC 30 providing overall concentrating power at the surface of the algae media of about 4.
  • bioreactor I was oriented horizontally and contained mechanical mixing means
  • bioreactor II was oriented perpendicularly to solar orbit and contained air mixing means.

Abstract

Ce bioréacteur (2) permettant une productivité améliorée d'algues photosynthétiques comprend un logement tubulaire (4) entourant une enveloppe tubulaire (6) située dans celui-ci et définissant entre l'enveloppe et lui-même un espace (18) destiné à être rempli de fluide. Le logement (4) et l'enveloppe (6) sont fabriqués dans au moins un matériau transparent et possèdent chacun un orifice d'entrée (20, 16) ainsi qu'un orifice de sortie (22, 28) lesquels constituent un accès à l'espace (18) ainsi qu'à l'intérieur de l'enveloppe (6), un mélangeur (8) destiné à mélanger des milieux d'algues étant placé dans l'enveloppe (6). L'invention concerne également un système de réaction biologique dans lequel l'enveloppe (6) contient un fluide possédant un indice de réfraction sélectif et dans lequel, en fonction d'un rapport géométrique donné entre le logement (4) et l'enveloppe (6), la puissance de concentration du rayonnement est régulée par la modification de l'indice de réfraction du fluide.
PCT/US1995/000019 1994-01-12 1995-01-10 Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques WO1995019424A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU15969/95A AU681243B2 (en) 1994-01-12 1995-01-10 A bioreactor and system for improved productivity of photosynthetic algae
US08/669,320 US5958761A (en) 1994-01-12 1995-01-10 Bioreactor and system for improved productivity of photosynthetic algae
JP7519032A JPH09507390A (ja) 1994-01-12 1995-01-10 光合成藻類の生産性を改善するバイオリアクター及びシステム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL10832194A IL108321A (en) 1994-01-12 1994-01-12 Bioreactor and system for improved productivity of photosynthetic algae
IL108,321 1994-01-12

Publications (1)

Publication Number Publication Date
WO1995019424A1 true WO1995019424A1 (fr) 1995-07-20

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ID=11065701

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/000019 WO1995019424A1 (fr) 1994-01-12 1995-01-10 Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques

Country Status (4)

Country Link
JP (1) JPH09507390A (fr)
AU (1) AU681243B2 (fr)
IL (1) IL108321A (fr)
WO (1) WO1995019424A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021723A1 (fr) * 1995-01-13 1996-07-18 Enviro Research Pty. Ltd. Appareil de production de biomasse
WO1997011154A1 (fr) * 1995-09-23 1997-03-27 Michael Melkonian Photo-bioreacteur solaire rotatif pour la production de biomasse d'algues a partir notamment de gaz contenant du dioxyde de carbone
WO1999020736A1 (fr) * 1997-10-22 1999-04-29 Stephen Charles Skill Photoreaction
WO2002099031A1 (fr) * 2001-06-01 2002-12-12 Yamaha Hatsudoki Kabushiki Kaisha Dispositif et procede permettant de cultiver des micro-algues
WO2010014010A2 (fr) * 2008-08-01 2010-02-04 Algae-Tech Ltd Système de culture d’algues
FR2985269A1 (fr) * 2011-12-29 2013-07-05 Dominique Patrick Delobel Dispositif d'agitation de bassin d'aquaculture de micro organismes photosynthetiques
WO2014093444A1 (fr) * 2012-12-11 2014-06-19 Atmi Packaging, Inc. Récipient pour la culture cellulaire

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006282946A1 (en) * 2005-08-25 2007-03-01 Solix Biofuels, Inc. Method, apparatus and system for biodiesel production from algae

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2715795A (en) * 1954-06-22 1955-08-23 Basic Res Corp Microorganism culture method and apparatus
US3303608A (en) * 1965-09-02 1967-02-14 Patrick J Hannan Oxygen production by photosynthesis of algae under pressure
US3986297A (en) * 1974-06-24 1976-10-19 Shoji Ichimura Photosynthesis reactor tank assembly
US4324068A (en) * 1980-03-03 1982-04-13 Sax Zzyzx, Ltd. Production of algae
JPS5851888A (ja) * 1981-06-18 1983-03-26 リンステイチユ−ト・ナシヨナル・デ・ラ・リサ−チ・サイエンテイフイツク 藻類および非光合成微生物類の透析培養方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3984297A (en) * 1974-04-15 1976-10-05 W. R. Grace & Co. Curable polyene-polythiol hydantoin compositions

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2715795A (en) * 1954-06-22 1955-08-23 Basic Res Corp Microorganism culture method and apparatus
US3303608A (en) * 1965-09-02 1967-02-14 Patrick J Hannan Oxygen production by photosynthesis of algae under pressure
US3986297A (en) * 1974-06-24 1976-10-19 Shoji Ichimura Photosynthesis reactor tank assembly
US4324068A (en) * 1980-03-03 1982-04-13 Sax Zzyzx, Ltd. Production of algae
JPS5851888A (ja) * 1981-06-18 1983-03-26 リンステイチユ−ト・ナシヨナル・デ・ラ・リサ−チ・サイエンテイフイツク 藻類および非光合成微生物類の透析培養方法

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021723A1 (fr) * 1995-01-13 1996-07-18 Enviro Research Pty. Ltd. Appareil de production de biomasse
US5846816A (en) * 1995-01-13 1998-12-08 Enviro Research Pty Ltd. Apparatus for biomass production
WO1997011154A1 (fr) * 1995-09-23 1997-03-27 Michael Melkonian Photo-bioreacteur solaire rotatif pour la production de biomasse d'algues a partir notamment de gaz contenant du dioxyde de carbone
AU704463B2 (en) * 1995-09-23 1999-04-22 Michael Melkonian Rotating solar photo bioreactor for use in the production of algal biomass from gases, in particular CO2-containing gases
WO1999020736A1 (fr) * 1997-10-22 1999-04-29 Stephen Charles Skill Photoreaction
US6370815B1 (en) 1997-10-22 2002-04-16 Stephen Skill Photoreaction
WO2002099031A1 (fr) * 2001-06-01 2002-12-12 Yamaha Hatsudoki Kabushiki Kaisha Dispositif et procede permettant de cultiver des micro-algues
WO2010014010A2 (fr) * 2008-08-01 2010-02-04 Algae-Tech Ltd Système de culture d’algues
WO2010014010A3 (fr) * 2008-08-01 2010-07-01 Algae-Tech Ltd Système de culture d’algues
US9688951B2 (en) 2008-08-01 2017-06-27 Algae-Tech Ltd. Algae growth system
FR2985269A1 (fr) * 2011-12-29 2013-07-05 Dominique Patrick Delobel Dispositif d'agitation de bassin d'aquaculture de micro organismes photosynthetiques
WO2014093444A1 (fr) * 2012-12-11 2014-06-19 Atmi Packaging, Inc. Récipient pour la culture cellulaire
US10280391B2 (en) 2012-12-11 2019-05-07 Pall Technology Uk Limited Recipient for cell cultivation
US10781417B2 (en) 2012-12-11 2020-09-22 Pall Technology Uk Limited System and method for detachment of cells in fixed bed reactors

Also Published As

Publication number Publication date
IL108321A (en) 1998-10-30
AU681243B2 (en) 1997-08-21
AU1596995A (en) 1995-08-01
IL108321A0 (en) 1994-04-12
JPH09507390A (ja) 1997-07-29

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