WO1995011587A1 - Soilless propagation method and device - Google Patents
Soilless propagation method and device Download PDFInfo
- Publication number
- WO1995011587A1 WO1995011587A1 PCT/BE1994/000071 BE9400071W WO9511587A1 WO 1995011587 A1 WO1995011587 A1 WO 1995011587A1 BE 9400071 W BE9400071 W BE 9400071W WO 9511587 A1 WO9511587 A1 WO 9511587A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tuberization
- cuttings
- carried out
- vitro
- seedlings
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Definitions
- the present invention relates to a process for obtaining plant reproductive organs combining in vitro culture and above-ground multiplication of seedlings, bulbs, bulbils, rhizomes and tubers (minitubercules). This process finds an application in obtaining seed or pre-base plants of irreproachable phytosanitary quality, in particular in the context of the cultivation of potatoes.
- the invention also relates to a device for implementing the method.
- Processes of multiplication and tuberization are known by in vitro techniques, the only techniques which can currently guarantee the production of seedlings, tubers or other organs of vegetative reproduction free of pathogenic agents such as viruses, viroids, bacteria, fungi or nematodes by example.
- microplantules obtained in vitro are either acclimatized in a greenhouse, or maintained in in vitro culture for the production of reproductive organs (bulbs or microtubercules).
- microtubers In the particular case of the potato, for example, the methods of in vitro production of microtubers have been described frequently, which consist in stimulating tuberization after several weeks on the stems of microplantules by transferring the microplantules into a nutritive medium, generally used in liquid form, containing a higher concentration of sucrose than in multiplication media and / or containing a complement of biologically active substances such as hormones (cytokinins), growth retardants (CCC), absorbers ethylene or by applying carbon dioxide C0 2 (patent EP 476,141), the seedlings then being placed under a diffuse light or at
- micro-tubers These processes are characterized by a low yield of micro-tubers, by small microtubers (2 to 7 mm in diameter), by a high dormancy of 4 to G months and by a limited reserve of organic and mineral substances in them . They are often cultivated in a greenhouse before sowing in the field in order to obtain mini-tubers.
- Patent EP-A-0 476 141 Process for producing tuber (1991).
- mini-tubers obtained after a subculture in a greenhouse or under shelter of the seedlings or microtubers from Vin vitro.
- techniques have been proposed for increasing the rate of production of minitubercules in soil (patent WO 88/04137) by transplanting propagated plants to the greenhouse and having undergone an induction treatment in vitro. The plants are treated with a culture medium containing hormonal additives, in order to promote the development of mini-tubers, which can be harvested G at 8 weeks after transplantation.
- plants are never immune to contamination, since the culture is done either in soil or in growing medium in the greenhouse.
- the method according to the invention makes it possible to increase the crop produced by seedling, to reduce the duration of the process of obtaining this crop, to arrive precisely at a seed with the desired diameter, to ensure the aptitude for seeding in the open ground and obtaining crops all year round by radically modifying the propagation techniques and the known conditions of implementation.
- the present invention consists in multiplying and inducing above-ground tuberization by an aero-hydroponic technique, to replace the techniques of in vitro culture in the multiplication and tuberization phases.
- This new aero-hydroponic technique for producing seedlings, bulbs, bulbils, rhizomes and tubers, guarantees quality health of equipment at costs significantly lower than those of products derived from in vitro techniques.
- the method according to the invention is characterized in that it comprises a first step of in vitro propagation of cuttings from the merisrasi, a second step of multiplication and elongation of said cuttings in order to obtain seedlings then a third step of tuberization of said seedlings, the second and third stages being carried out in aero-hydroponics with a nutritive solution substantially identical to the solutions suitable for the conditions of culture in vitro, with the proviso that it is substantially free of an assimilable carbon source.
- meristem is generally meant the plant tissue formed of undifferentiated cells, seat of rapid and numerous divisions, located in the growth regions of the plant.
- assistant carbon source in particular all the sugars, in particular sucrose, glucose.
- the amount possibly present is very much lower than that normally used in solutions for in vitro cultures. At least 10 times less. Preferably at least, 50 times less.
- the solution may not contain said assimilable carbon source at all. In other words, plants are such that they work in autotrophic conditions.
- the nutrient solution is also remarkable for the fact that it contains an effective amount of vitamin, in particular thiamine HC1, pyridoxine, glycine, casein hydrorysat.
- this composition will include the anions and cations commonly used, in particular the ingredients of the formula of Muraschige and Skoog (1962) added with a mixture of amino acids obtained by acid hydrolysis of casein, for example the cations, potassium , calcium, magnesium, sodium, iron, copper, zinc, cobalt, nitrate, sulfate anions etc.
- this composition will comprise in part by weight: Ammonium nitrate 1650 mg
- Nicotinic acid 0.5 mg
- hydro-hydroponic denotes a method of hydroponic culture in which the water loaded with different additives which allow the growth of plants is conveyed in nebulized form.
- the cuttings have a portion of stem and carry at least one node, preferably two nodes, that is to say approximately 10 to 20 weeks after the differentiation of the meristems, these are subjected to the step of multiplication and elongation.
- a first multiplication can be carried out in vitro using techniques identical to those described in the above-mentioned prior art.
- the cuttings obtained at the end of the first phase are placed on a support, in particular in synthetic foam, preferably sheets of polyphenol foam, under suitable lighting in particular from 60 to 150 ⁇ E / m 2 / s at 16 hours of light, a knot that can be inserted into the openings for this purpose in the support.
- the cuttings are cultivated on the support until at least one axillary stem has been formed, the apical portion of which is transplanted in order to obtain a complete seedling comprising at least five nodes.
- the intermediate portions of the axillary rod comprising at least one node are transplanted to form new axillary rods.
- the apical and intermediate portions of the new stems are treated as above: this operation is repeated the appropriate number of times.
- the apical buds are removed approximately two to three weeks after transplanting.
- the nutritive solution, during the second stage, is that used for the propagation of the cuttings, possibly modified by additives to favor the elongation of the cuttings and the appearance of the axillary stems, in particular benzyladenine (B, A) and 2 -isopentenyladenine (2-iP).
- B, A benzyladenine
- 2-iP 2 -isopentenyladenine
- the tuberization stage can be carried out according to two methods. According to a first method, the tuberization stage is carried out with a nutritive solution of reduced content of nitrogenous compound and under conditions of short days or long days, depending on the physiology of the variety used.
- the leaves of the pre-induced plants are removed with a portion of the stem and transplanted onto the support.
- the tuberization is carried out with a nutritive solution comprising the elements described previously with the exception of ammonium nitrate and disodium EDTA.
- the method according to the invention is particularly suitable for obtaining mini-tubers of potatoes.
- a variant of this process relating to the cultivation of potato cuttings is described below.
- Cuttings of seed potatoes from meristems in vitro are placed on polyphenol foam plates in an enclosure fed by spraying with a culture medium containing the components of the culture medium used for the initial propagation of the seedlings, optionally supplemented by additives promoting growth and transfer into non-sterile conditions of explants.
- Each cutting comprises a portion of stem carrying two nodes, one of them being pressed into the polyphenol foam support.
- the cuttings are placed under a lighting of 60 to 150 ⁇ E / mVs at 16 hours of light.
- the seedlings After three weeks, the seedlings have formed one or two axillary stems with at least five nodes.
- the axillary stems obtained are divided into three: two portions comprising two nodes and a portion comprising a node and the apex.
- the portions comprising two nodes are transplanted as above and form the cuttings of type A.
- the apical portion, also transplanted in the support in polyphenol, constitutes a cuttings of type D.
- the type A cuttings can be successively multiplied while the type D cuttings, form after 15 days a complete seedling, of a size greater than 15 cm, comprising more than five nodes.
- the elongated plants of a size greater than 15 cm are transplanted into a new support under conditions of short days at 8 hours of light under a lighting of 60-150 ⁇ E / n.2 / s for 10 to 21 days.
- various inducers of tuberization can be used (jasmonic acid, salicilic acid and derivatives, coumarin, ancymidol as well as growth retardants (CCC, Alar, TIBA) or ethylene absorbing agents such as permanganate.
- Alar succinic acid 2-2 dimethyl hydrazine
- CCC chlorocholine hydrochloride
- carbon dioxide (CO 2 ) at a concentration of 1 to 40% can also be applied in the culture chambers, in particular at the level of the roots after the induction phase of the tuberization, the plants can be maintained in short days on a medium of the same composition, except for a reduced content of nitrogen compound.
- the minitubercules therefore form, after six to nine weeks, on the runners which develop in the lower part of the aero-hydroponic device.
- the leaves of the pre-induced plants are removed and cut with a portion of stem, to be transplanted, vertically on the polyphenol substrate.
- the plants are placed in continuous light or in long day, under aero-hydroponic conditions, according to the procedure described by EWING E.E. on leaves of plants in culture in vitro (Potato physiology, 1985).
- the induced tuberization on the seedlings or leaves is carried out in an air-conditioned room by modifying the photoperiod and the temperature.
- the tubers are harvested as soon as they have reached the desired size.
- the harvest continues for 10 to 12 weeks, depending on the species or variety. Continuous removal of part of the tubers promotes tuberization.
- the photoperiod for the tuberization of the seed potatoes is then 12 to 20 hours for a temperature of 15 to 25 ° C, depending on the variety used.
- 1 or 2 mini-tubers are collected at the base of each leaf after 15 to 18 days.
- the multiplication and tuberization cycles for the seed potatoes are carried out in air-conditioned rooms which eliminate any risk of contamination by aphids.
- the nutritive solution recycled and subjected to UV radiation as well as the absence of soil or potting soil guarantees the sanitary property of the products.
- the apparatuses used for the culture above-ground can be of different types.
- a device particularly suitable for implementing the method according to the invention comprises a container on which a culture support is placed, said container containing inside a nebulizer, connected to a means for supplying a nutritive solution and the container being connected to a means for discharging the nutrient solution, said supply and discharge means being connected to a nutrient solution tank and to one or more control means.
- FIG. 1 represents a vertical section of a production channel and the figure 2 shows schematically a device for implementing the method according to the invention.
- Figure 1 shows a cross section of a channel 2 for producing seedlings 1 and tubers or bulbils 18.
- the nutrient solution is distributed by means of line 17 and is nebulized at the root part of the seedlings.
- the seedlings 1 are arranged above a channel 2, on and through a support 16, their lower parts being in the channel, and are fed 23 periodically with a solution mist.
- nutrient pressurized by a pump 4 and distributed by a nebulizer 17.
- the excess solution is recycled 24 and sterilized by a UV device before returning to the tank 5 containing the nutrient solution 3.
- the tubers or bulbils produced will appear in channel 2 and are fed in the same way as the seedlings 1. Compensation for losses of reservoir 5, in water and nutrients is done automatically, by level sensors 6 and / or concentration 7 and 8 and regulating organs 9 and 10.
- a group of metering pumps 14 pours into the reservoir 5 of nutritive solution 3 the quantities of acid and nutrients 11 and 12 calculated by the regulators 9 and 10.
- a sensor 21 making it possible to measure the concentration of C0 2 acts on a regulating valve 20 which injects, from a compressed CO 2 reservoir 22, the required quantities of CO 2 into the channel 2 for producing seedlings.
- the control unit 15 manages the correction of the pH, the conductivity of the CO 2 concentration and other level. It can also manage the entire circuit in all phases of the process.
- the multiplication is carried out in an air-conditioned room at a temperature of 20-24 ° C, the cuttings receive an assimilation illumination of approximately 60-100 ⁇ E / mVs, thanks to fluorescent tubes, with a photoperiod of 16 hours.
- Each transplanting of autotrophic cuttings is carried out at regular intervals.
- the culture medium is renewed every three weeks.
- the aim of the test was to compare the quality of the potato seedlings and the yield of the micropropagation carried out according to the process of the invention on supports of polyphenol foam and in autotrophic condition, compared with the conventional technique in vitro.
- the yields as a function of the type of cuttings cultivated were compared: a) 1 node provided with an axillary bud inserted into the culture substrate, b) 1 node provided with an axillary bud placed outside the substrate of culture, c) 1 upper node provided with a terminal bud.
- This test is carried out with a view to highlighting the advantage of developing an automatic method of cutting or chopping seedlings to transplant under industrial production conditions.
- the culture support is brought to saturation, then the spraying of the culture medium is stopped.
- the capillary retention of the substrate used makes it possible to space two saturation cycles over a period of 8 hours.
- Lengthening, vigor, leaf development and growth speed are favored in non-sterile conditions.
- the conditions of culture were the same (temperature of 24 ° C and light of 60 ⁇ E / mVs for a photoperiod of 16 hours.
- Plants obtained under non-sterile conditions had, on average, a height of 10.9 cm of had 9.5 leaves while those cultivated under sterile conditions had a height of 9.4 cm and had 6.3 leaves.
- transplanting process and the type of explant determines the quality of the plants obtained at the end of the culture.
- Type of cuttings put total length of the Average number of P u rce n of Coefficient of in crop planting (cm) leaves per seedling developed seedlings multiplication (*) after transplanting
- the multiplication coefficient is defined as the number of axillary buds developed per cutting, after transplanting.
- the apical segments also allow a multiplication greater than or equal to 4 in two weeks of culture.
- the supports were placed at a temperature of 23 "C under the condition of short days (8 hours of light). Under these conditions, after three weeks of incubation, the formation of minitubercules is observed on the stems of the plants located in the part bottom of the device, kept in the dark
- the quantity of minitubers produced was between 0.5 to 3 tubers per plant for a size varying from 0.7 to 1.5 cm.
- Tests for the production of minitubers were carried out by comparing techniques 1 and 2 described above. Plants from type A and type D cuttings were placed in induction conditions in short days (8 hours of light) for 3 weeks on the tuberization medium, then placed under 20 hours of light, at a temperature of
- Method 1 Type A cuttings 59.6 225 plants whole plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Hydroponics (AREA)
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP94928735A EP0726702A1 (en) | 1993-10-27 | 1994-10-21 | Soilless propagation method and device |
AU78056/94A AU7805694A (en) | 1993-10-27 | 1994-10-21 | Soilless propagation method and device |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE9301152A BE1007666A3 (en) | 1993-10-27 | 1993-10-27 | MULTIPLICATION PROCESS ABOVE GROUND OF SEEDLINGS, BULBS, bulbils, RHIZOMES AND TUBERS. |
BE9301152 | 1993-10-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995011587A1 true WO1995011587A1 (en) | 1995-05-04 |
Family
ID=3887480
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/BE1994/000071 WO1995011587A1 (en) | 1993-10-27 | 1994-10-21 | Soilless propagation method and device |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0726702A1 (en) |
AU (1) | AU7805694A (en) |
BE (1) | BE1007666A3 (en) |
WO (1) | WO1995011587A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0887013A1 (en) * | 1996-10-14 | 1998-12-30 | THE JAPAN TOBACCO & SALT PUBLIC CORPORATION | Method for producing potato tubers |
WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
NL2004976C2 (en) * | 2010-06-28 | 2011-12-29 | Living Foods B V | SYSTEM, CASH AND METHOD FOR GROWING AND / OR INCREASING PRODUCTS. |
WO2012059155A1 (en) * | 2010-11-03 | 2012-05-10 | Horticoop B.V. | Method of growing one or more plants |
WO2012110065A1 (en) * | 2011-02-16 | 2012-08-23 | Horticoop B.V. | Device and method for growing one or more plants |
WO2012136232A1 (en) * | 2011-04-06 | 2012-10-11 | Horticoop B.V. | Device and method for growing one or more plants |
WO2016147577A1 (en) * | 2015-03-19 | 2016-09-22 | パナソニックIpマネジメント株式会社 | Hydroponic culture device |
US10058040B2 (en) | 2010-09-17 | 2018-08-28 | Kamal Daas | Apparatus and method for growing one or more plants |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4332105A (en) * | 1976-07-26 | 1982-06-01 | Adi-Aeroponics Growth Ltd. | Apparatus and method for plant growth in aeroponic conditions |
WO1988004137A1 (en) * | 1986-12-01 | 1988-06-16 | Novotrade Rt. | An effective process for the in vitro-in vivo production of potato minitubers |
EP0293488A1 (en) * | 1986-12-02 | 1988-12-07 | Kyowa Hakko Kogyo Co., Ltd. | Method of multiplying tubers |
WO1989010399A1 (en) * | 1988-04-29 | 1989-11-02 | Wisconsin Alumni Research Foundation | Microtuber propagation of potatoes |
EP0388109A1 (en) * | 1989-03-11 | 1990-09-19 | Korea Institute Of Science And Technology | Potato Production |
EP0476141A1 (en) * | 1990-03-23 | 1992-03-25 | Kirin Beer Kabushiki Kaisha | Process for producing tuber |
US5136804A (en) * | 1988-10-20 | 1992-08-11 | Shira Aeroponics (1984) Ltd. | System for germination, propagation and growing plants in ultrasonic-fog conditions (aeroponics) |
-
1993
- 1993-10-27 BE BE9301152A patent/BE1007666A3/en not_active IP Right Cessation
-
1994
- 1994-10-21 WO PCT/BE1994/000071 patent/WO1995011587A1/en not_active Application Discontinuation
- 1994-10-21 AU AU78056/94A patent/AU7805694A/en not_active Abandoned
- 1994-10-21 EP EP94928735A patent/EP0726702A1/en not_active Withdrawn
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4332105A (en) * | 1976-07-26 | 1982-06-01 | Adi-Aeroponics Growth Ltd. | Apparatus and method for plant growth in aeroponic conditions |
WO1988004137A1 (en) * | 1986-12-01 | 1988-06-16 | Novotrade Rt. | An effective process for the in vitro-in vivo production of potato minitubers |
EP0293488A1 (en) * | 1986-12-02 | 1988-12-07 | Kyowa Hakko Kogyo Co., Ltd. | Method of multiplying tubers |
WO1989010399A1 (en) * | 1988-04-29 | 1989-11-02 | Wisconsin Alumni Research Foundation | Microtuber propagation of potatoes |
US5136804A (en) * | 1988-10-20 | 1992-08-11 | Shira Aeroponics (1984) Ltd. | System for germination, propagation and growing plants in ultrasonic-fog conditions (aeroponics) |
EP0388109A1 (en) * | 1989-03-11 | 1990-09-19 | Korea Institute Of Science And Technology | Potato Production |
EP0476141A1 (en) * | 1990-03-23 | 1992-03-25 | Kirin Beer Kabushiki Kaisha | Process for producing tuber |
Non-Patent Citations (5)
Title |
---|
"In vitro plant tissue culture and its agricultural applications", 1966, BUTTERWORTHS, LONDON * |
"Potato physiology", 1985, ACADEMIC PRESS, NEW YORK * |
G.HUSSEY & N.J.STACEY: "Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.)", ANNALS OF BOTANY, vol. 53, 1984, pages 565 - 578 * |
P.C.DEBERGH & R.H.ZIMMERMAN -EDS-: "Micropropagation; technology and application", 1991, KLUWER ACADEMIC PUBL., DORDRECHT NL * |
Y.P.S.BAJAJ: "Biotechnology in agriculture and forestry Vol. 19. High tech and micropropagation III", 1992, SPRINGER VERLAG, BERLIN * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0887013A1 (en) * | 1996-10-14 | 1998-12-30 | THE JAPAN TOBACCO & SALT PUBLIC CORPORATION | Method for producing potato tubers |
EP0887013A4 (en) * | 1996-10-14 | 1999-08-25 | Japan Tobacco & Salt Public | Method for producing potato tubers |
WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
NL2004976C2 (en) * | 2010-06-28 | 2011-12-29 | Living Foods B V | SYSTEM, CASH AND METHOD FOR GROWING AND / OR INCREASING PRODUCTS. |
US10058040B2 (en) | 2010-09-17 | 2018-08-28 | Kamal Daas | Apparatus and method for growing one or more plants |
WO2012059155A1 (en) * | 2010-11-03 | 2012-05-10 | Horticoop B.V. | Method of growing one or more plants |
US8887439B2 (en) | 2010-11-03 | 2014-11-18 | Horticoop B.V. | Method for cultivating one or more plants |
WO2012110065A1 (en) * | 2011-02-16 | 2012-08-23 | Horticoop B.V. | Device and method for growing one or more plants |
WO2012136232A1 (en) * | 2011-04-06 | 2012-10-11 | Horticoop B.V. | Device and method for growing one or more plants |
WO2016147577A1 (en) * | 2015-03-19 | 2016-09-22 | パナソニックIpマネジメント株式会社 | Hydroponic culture device |
Also Published As
Publication number | Publication date |
---|---|
BE1007666A3 (en) | 1995-09-12 |
AU7805694A (en) | 1995-05-22 |
EP0726702A1 (en) | 1996-08-21 |
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