WO1995010591A1 - Protease-containing cleaning compositions - Google Patents
Protease-containing cleaning compositions Download PDFInfo
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- WO1995010591A1 WO1995010591A1 PCT/US1994/011776 US9411776W WO9510591A1 WO 1995010591 A1 WO1995010591 A1 WO 1995010591A1 US 9411776 W US9411776 W US 9411776W WO 9510591 A1 WO9510591 A1 WO 9510591A1
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- subtilisin
- protease
- amino acid
- alkyl
- protease enzyme
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- 0 CCC(*)*=CCc1cc(C)ccc1 Chemical compound CCC(*)*=CCc1cc(C)ccc1 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
- A61K8/416—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/22—Peroxides; Oxygen; Ozone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/463—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfuric acid derivatives, e.g. sodium lauryl sulfate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- A61Q11/02—Preparations for deodorising, bleaching or disinfecting dentures
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q40/00—Finance; Insurance; Tax strategies; Processing of corporate or income taxes
- G06Q40/04—Trading; Exchange, e.g. stocks, commodities, derivatives or currency exchange
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/22—Gas releasing
- A61K2800/222—Effervescent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
Definitions
- the present invention relates to a variety of cleaning compositions comprising novel protease enzymes which are carbonyl hydrolase variants.
- BACKGROUND Enzymes make up the largest class of naturally occurring proteins. Each class of enzyme generally catalyzes (accelerates a reaction without being consumed) a different kind of chemical reaction.
- One class of enzymes known as proteases, are known for their ability to hydrolyze (break down a compound into two or more simpler compounds with the uptake of the H and OH parts of a water molecule on either side of the chemical bond cleaved) other proteins. This ability to hydrolyze proteins has been taken advantage of by incorporating naturally occurring and protein engineered proteases as an additive to laundry detergent preparations. Many stains on clothes are proteinaceous and wide- specificity proteases can substantially improve removal of such stains.
- protease characteristics such as thermal stability, pH stability, oxidative stability and substrate specificity are not necessarily optimized for utilization outside the natural environment of the enzyme.
- the amino acid sequence of the protease enzyme determines the characteristics of the protease.
- a change of the amino acid sequence of the protease may alter the properties of the enzyme to varying degrees, or may even inactivate the enzyme, depending upon the location, nature and/or magnitude of the change in the amino acid sequence.
- Several approaches have been taken to alter the amino acid sequence of proteases in an attempt to improve their properties, with the goal of increasing the efficacy of the protease for cleaning uses such as in the wash environment. These approaches include altering the amino acid sequence to enhance thermal stability and to improve oxidation stability under quite diverse conditions.
- proteases useful for cleaning a variety of surfaces. It is therefore an object of the present invention to provide cleaning compositions containing protease enzymes which are carbonyl hydrolase variants having improved proteolytic activity, substrate specificity, stability and/or enhanced performance characteristics.
- protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position +76 in combination with one or more of the following residues: +99, +101 , +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered positions corresponds to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin); and
- the present invention also relates to methods for cleaning items in need of cleaning by contacting said item with a protease enzyme which is a carbonyl hydrolase variant as described herein.
- the invention therefore encompasses a method for cleaning fabrics comprising contacting, preferably with agitation, said fabrics with an aqueous liquor containing said protease enzyme.
- the method can be carried out at temperatures below about 60°C but, of course, is quite effective at laundry temperatures up to the boil.
- the present invention also relates to a method for cleaning dishes by contacting a dish in need of cleaning with a protease enzyme as described herein.
- the present invention methods also include methods for personal cleansing , said methods comprising contacting the part of the human or lower animal body in need of cleaning with a protease enzyme as described herein.
- FIGs. 1 A-C depict the DNA and amino acid sequence for Bacillus amyloliquefaciens subtilisin and a partial restriction map of this gene (Seq. ID No.6).
- Fig. 2 depicts the conserved amino acid residues among subtilisins from Bacillus amyloliquefaciens (BPN)' and Bacillus lentus (wild-type).
- Figs. 3A and 3B depict the amino acid sequence of four subtilisins.
- the top line represents the amino acid sequence of subtilisin from Bacillus amyloliquefaciens subtilisin (also sometimes referred to as subtilisin BPN') (Seq. ID No.7).
- the second line depicts the amino acid sequence of subtilisin from Bacillus subtilis (Seq. ID No.8).
- the third line depicts the amino acid sequence of subtilisin from B. licheniformis (Seq. ID No.9).
- the fourth line depicts the amino acid sequence of subtilisin from Bacillus lentus (also referred to as subtilisin 309 in PCT WO89/06276) (Seq. ID No.10).
- the symbol * denotes the absence of specific amino acid residues as compared to subtilisin BPN'.
- Fig. 4 depicts the construction of plasmid GGA274.
- Fig. 5 depicts the construction of GGT274 which is an intermediate to certain expression plasmids used in this application.
- Figs. 6A and 6B depict the DNA and amino acid sequence of subtilisin from Bacillus lentus (Seq. ID No.11 ).
- the mature subtilisin protein is coded by the codons beginning at the codon GCG (334-336) corresponding to Ala.
- Figs. 7A and 7B depict the DNA and amino acid sequence of a preferred embodiment of the invention (N76D/S103A ⁇ 104I) (Seq. ID No.12).
- the DNA in this figure has been modified by the methods described to encode aspartate at position 76, alanine at position 103 and isoleucine at position 104.
- the mature subtilisin variant protein is coded by the codons beginning at the codon GCG (334-336) corresponding to Ala.
- Fig. 8 depicts the construction of vector pBCDAICAT.
- Fig. 9 depicts the construction of vector pUCCATFNA.
- Fig. 10 shows the stability of a preferred mutant enzyme compared to wild-type, in a liquid detergent formulation.
- the invention includes protease enzymes which are non-naturally- occurring carbonyl hydrolase variants having a different proteolytic activity, stability, substrate specificity, pH profile and/or performance characteristic as compared to the precursor carbonyl hydrolase from which the amino acid sequence of the variant is derived.
- the precursor carbonyl hydrolase may be a naturally-occurring carbonyl hydrolase or recombinant hydrolase.
- such carbonyl hydrolase variants have an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids.
- the plurality of amino acid residues of the precursor enzyme correspond to position +76 in combination with one or more of the following residues +99, +101 , +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered position corresponds to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins, such as Bacillus lentus subtilisin.
- the carbonyl hydrolase variants which are protease enzyme useful in the present invention compositions comprise replacement of amino acid residue +76 in combination with one or more additional modifications.
- the variant protease enzymes useful for the present invention comprise the substitution, deletion or insertion of amino acid residues in the following combinations: 76/99; 76/101; 76/103; 76/104; 76/107; 76/123; 76/99/101; 76/99/103; 76/99/104; 76/101/103; 76/101/104; 76/103/104; 76/104/107; 76/104/123; 76/107/123; 76/99/101/103; 76/99/101/104; 76/99/103/104; 76/101/103/104; 76/103/104/123; 76/104/107/123; 76/99/101/103; 76/99/101/104; 76/99/103/104; 76/101/103/104; 76/103/104/123; 76/
- variant enzymes useful for the present invention comprise the substitution, deletion or insertion of an amino acid residue in the following combination of residues: 76/99; 76/104; 76/99/104; 76/103/104; 76/104/107; 76/101/103/104; 76/99/101/103/104 and 76/101/104 of B. amyloliquefaciens subtilisin.
- Variant DNA sequences encoding such carbonyl hydrolase or subtilisin variants are derived from a precursor DNA sequence which encodes a naturally-occurring or recombinant precursor enzyme.
- the variant DNA sequences are derived by modifying the precursor DNA sequence to encode the substitution of one or more specific amino acid residues encoded by the precursor DNA sequence corresponding to positions 76, 99, 101, 103, 104, 107, 123, 27, 105, 109, 126, 128, 135, 156, 166, 195, 197, 204, 206, 210, 216, 217, 218, 222, 260, 265 and/or 274, in Bacillus amyloliquefaciens or any combination thereof.
- the amino acid residues identified for modification herein are identified according to the numbering applicable to B. amyloliquefaciens (which has become the conventional method for identifying residue positions in all subtilisins), the preferred precursor DNA sequence useful for the present invention is the DNA sequence of Bacillus lentus as shown in Fig. 6 (Seq. ID No. 11).
- variant DNA sequences encode the insertion or substitution of the amino acid residue 76 in combination with one or more additional modification.
- the variant DNA sequences encode the substitution or insertion of amino acid residues in the following combinations: 76/99; 76/101 ; 76/103; 76/104; 76/107; 76/123; 76/99/101; 76/99/103; 76/99/104; 76/101/103; 76/101/104; 76/103/104; 76/104/107; 76/104/123; 76/107/123; 76/99/101/103; 76/99/101/104; 76/99/103/104; 76/101/103/104; 76/103/104/123; 76/104/107/123; 76/99/101/103/104; 76/99/103/104; 76/101/103/104; 76/103/104/123; 76/104/107/123; 76/99/101/103/104; 76/99/103/104/123; 76
- variant DNA sequences encode for the modification of the following combinations of residues: 76/99; 76/104; 76/99/104; 76/103/104; 76/104/107; 76/101 /103/104; 76/99/101 /103/104 and 76/101 /104.
- These recombinant DNA sequences encode carbonyl hydrolase variants having a novel amino acid sequence and, in general, at least one property which is substantially different from the same property of the enzyme encoded by the precursor carbonyl hydrolase DNA sequence. Such properties include proteolytic activity, substrate specificity, stability, altered pH profile and/or enhanced performance characteristics.
- protease enzymes useful herein encompass the substitution of any of the nineteen naturally occurring L-amino acids at the designated amino acid residue positions. Such substitutions can be made in any precursor subtilisin (procaryotic, eucaryotic, mammalian, etc.). Thoughout this application reference is made to various amino acids by way of common one- and three- letter codes. Such codes are identified in Dale, J.W. (1989), Molecular Genetics of Bacteria. John Wiley & Sons, Ltd., Appendix B.
- Amino Acid Preferred Amino Acid to Residue be Substituted/Inserted
- subtilisin variants exhibiting altered stability (e.g., modified autoproteolytic stability) over precursor subtilisins.
- Bacillus amyloliquefaciens subtilisin alone or in combination with each other and in any combination with +76 mutations, produce subtilisin variants exhibiting altered proteolytic activity, altered thermal stability, altered pH profile, altered substrate specificity and/or altered performance characteristics.
- Carbonyl hydrolases are protease enzymes which hydrolyze compounds containing
- Naturally-occurring carbonyl hydrolases principally include hydrolases, e.g., peptide hydrolases such as subtilisins or metal loproteases.
- Peptide hydrolases include ⁇ - aminoacylpeptide hydrolase, peptidylamino acid hydrolase, acyiamino hydrolase, serine carboxypeptidase, metallocarboxypeptidase, thiol proteinase, carboxylproteinase and metalloproteinase. Serine, metallo, thiol and acid proteases are included, as well as endo and exo-proteases.
- Recombinant carbonyl hydrolase refers to a carbonyl hydrolase in which the DNA sequence encoding the naturally-occurring carbonyl hydrolase is modified to produce a mutant DNA sequence which encodes the substitution, insertion or deletion of one or more amino acids in the carbonyl hydrolase amino acid sequence. Suitable modification methods are disclosed herein, and in U.S. Patent 4,760,025 (RE 34,606), U.S. Patent 5,204,015 and U.S. Patent 5,185,258, the disclosure of which are incorporated herein by reference.
- Subtilisins are bacterial or fungal carbonyl hydrolases which generally act to cleave peptide bonds of proteins or peptides.
- subtilisin means a naturally-occurring subtilisin or a recombinant subtilisin.
- a series of naturally-occurring subtilisins is known to be produced and often secreted by various microbial species. Amino acid sequences of the members of this series are not entirely homologous. However, the subtilisins in this series exhibit the same or similar type of proteolytic activity.
- This class of serine proteases shares a common amino acid sequence defining a catalytic triad which distinguishes them from the chymotrypsin related class of serine proteases.
- subtilisins and chymotrypsin related serine proteases both have a catalytic triad comprising aspartate, histidine and serine.
- subtilisin related proteases the relative order of these amino acids, reading from the amino to carboxy terminus, is aspartate-histidine-serine.
- the chymotrypsin related proteases the relative order, however, is histidine-aspartate-serine.
- subtilisin herein refers to a serine protease having the catalytic triad of subtilisin related proteases. Examples include but are not limited to the subtilisins identified in Fig. 3 herein.
- Recombinant subtilisin refers to a subtilisin in which the DNA sequence encoding the subtilisin is modified to produce a variant (or mutant) DNA sequence which encodes the substitution, deletion or insertion of one or more amino acids in the naturally-occurring subtilisin amino acid sequence.
- Suitable methods to produce such modification include those disclosed in U.S. Patent 4,760,025 (RE 34,606), U.S. Patent 5,204,015 and U.S. Patent 5,185,258.
- Non-human carbonyl hydrolases and the DNA encoding them may be obtained from many procaryotic and eucaryotic organisms. Suitable examples of procaryotic organisms include gram negative organisms such as E. coli or Pseudomonas and gram positive bacteria such as Micrococcus or Bacillus. Examples of eucaryotic organisms from which carbonyl hydrolase and their genes may be obtained include yeast such as Saccharomyces cerevisiae, fungi such as Aspergillus sp. and non-human mammalian sources such as, for example, bovine sp. from which the gene encoding the carbonyl hydrolase chymosin can be obtained.
- yeast such as Saccharomyces cerevisiae
- fungi such as Aspergillus sp.
- non-human mammalian sources such as, for example, bovine sp. from which the gene encoding the carbonyl hydrolase chymosin can be obtained.
- non-human carbonyl hydrolase as used herein has a functional definition which refers to carbonyl hydrolases which are associated, directly or indirectly, with procaryotic and eucaryotic sources.
- a “carbonyl hydrolase variant” has an amino acid sequence which is derived from the amino acid sequence of a “precursor carbonyl hydrolase.”
- the precursor carbonyl hydrolases include naturally- occurring carbonyl hydrolases (subtilisin) and recombinant carbonyl hydrolases (subtilisin).
- the amino acid sequence of the carbonyl hydrolase variant is "derived” from the precursor hydrolase amino acid sequence by the substitution, deletion or insertion of one or more amino acids of the precursor amino acid sequence.
- Such modification is of the "precursor DNA sequence” which encodes the amino acid sequence of the precursor carbonyl hydrolase (subtilisin) rather than manipulation of the precursor carbonyl hydrolase (subtilisin) enzyme per se. Suitable methods for such manipulation of the precursor DNA sequence include methods disclosed herein, as well as methods known to those skilled in the art (see, for example, EP 0328299,
- Bacillus amyloliquefaciens subtilisin are identified herein for mutation.
- the modified residues are selected from the following combinations: 76/99; 76/101 ; 76/103; 76/104;
- protease enzymes useful in the present invention are not limited to the mutation of this particular subtilisin but extends to precursor carbonyl hydrolases containing amino acid residues at positions which are "equivalent" to the particular identified residues in Bacillus amyloliquefaciens subtilisin.
- the precursor subtilisin is Bacillus lentus subtilisin and the substitutions, deletions or insertions are made at the equivalent amino acid residue in ⁇ . lentus corresponding to those listed above.
- a residue (amino acid) of a precursor carbonyl hydrolase is equivalent to a residue of Bacillus amyloliquefaciens subtilisin if it is either homologous (i.e., corresponding in position in either primary or tertiary structure) or analogous to a specific residue or portion of that residue in Bacillus amyloliquefaciens subtilisin (i.e., having the same or similar functional capacity to combine, react, or interact chemically).
- amino acid sequence of a precursor carbonyl hydrolase is directly compared to the Bacillus amyloliquefaciens subtilisin primary sequence and particularly to a set of residues known to be invariant in subtilisins for which sequence is known. Fig.
- Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis (carisbergensis) and Bacillus lentus are aligned to provide the maximum amount of homology between amino acid sequences. A comparison of these sequences shows that there are a number of conserved residues contained in each sequence. These conserved residues (as between BPN' and B. lentus) are identified in Fig. 2.
- subtilisin variant useful in the invention, however, the amino acid equivalent to +76 in Bacillus amyloliquefaciens subtilisin is substituted with aspartate (D).
- aspartate D
- Table II A comparison of all the amino acid residues identified herein for substitution versus the preferred substitution for each such position is provided in Table II for illustrative purposes. Table II
- Equivalent residues may also be defined by determining homology at the level of tertiary structure for a precursor carbonyl hydrolase whose tertiary structure has been determined by x-ray crystallography. Equivalent residues are defined as those for which the atomic coordinates of two or more of the main chain atoms of a particular amino acid residue of the precursor carbonyl hydrolase and Bacillus amyloliquefaciens subtilisin (N on N, CA on CA, C on C and O on O) are within 0.13nm and preferably 0.1 nm after alignment.
- Alignment is achieved after the best model has been oriented and positioned to give the maximum overlap of atomic coordinates of non-hydrogen protein atoms of the carbonyl hydrolase in question to the Bacillus amyloliquefaciens subtilisin.
- the best model is the crystallographic model giving the lowest R factor for experimental diffraction data at the highest resolution available.
- Equivalent residues which are functionally analogous to a specific residue of Bacillus amyloliquefaciens subtilisin are defined as those amino acids of the precursor carbonyl hydrolases which may adopt a conformation such that they either alter, modify or contribute to protein structure, substrate binding or catalysis in a manner defined and attributed to a specific residue of the Bacillus amyloliquefaciens subtilisin.
- residues of the precursor carbonyl hydrolase for which a tertiary structure has been obtained by x-ray crystallography
- the atomic coordinates of at least two of the side chain atoms of the residue lie with 0.13nm of the corresponding side chain atoms of Bacillus amyloliquefaciens subtilisin.
- the coordinates of the three dimensional structure of Bacillus amyloliquefaciens subtilisin are set forth in EPO Publication No. 0251 446 (equivalent to U.S. Patent Application SN 08/212,291, the disclosure of which is incorporated herein by reference) and can be used as outlined above to determine equivalent residues on the level of tertiary structure.
- the carbonyl hydrolase variants useful in the present invention include the mature forms of carbonyl hydrolase variants, as well as the pro- and prepro-forms of such hydrolase variants.
- the prepro-forms are the preferred construction since this facilitates the expression, secretion and maturation of the carbonyl hydrolase variants.
- Prosequence refers to a sequence of amino acids bound to the N- terminal portion of the mature form of a carbonyl hydrolase which when removed results in the appearance of the "mature” form of the carbonyl hydrolase. Many proteolytic enzymes are found in nature as translational proenzyme products and, in the absence of post-translational processing, are expressed in this fashion.
- a preferred prosequence for producing carbonyl hydrolase variants, specifically subtilisin variants is the putative prosequence of Bacillus amyloliquefaciens subtilisin, although other subtilisin prosequences may be used. In the Examples, the putative prosequence from the subtilisin from Bacillus lentus (ATCC 21536) is used.
- signal sequence refers to any sequence of amino acids bound to the N-terminal portion of a carbonyl hydrolase or to the N- terminal portion of a prohydrolase which may participate in the secretion of the mature or pro forms of the hydrolase.
- This definition of signal sequence is a functional one, meant to include all those amino acid sequences encoded by the N-terminal portion of the subtilisin gene or other secretable carbonyl hydrolases which participate in the effectuation of the secretion of subtilisin or other carbonyl hydrolases under native conditions.
- the protease enzymes useful for the present invention utilize such sequences to effect the secretion of the carbonyl hydrolase variants as described herein.
- a preferred signal sequence used in the Examples comprises the first seven amino acid residues of the signal sequence from Bacillus subtilis subtilisin fused to the remainder of the signal sequence of the subtilisin from Bacillus lentus (ATCC 21536).
- a "prepro" form of a carbonyl hydrolase variant consists of the mature form of the hydrolase having a prosequence operably linked to the amino terminus of the hydrolase and a "pre” or “signal” sequence operably linked to the amino terminus of the prosequence.
- “Expression vector” refers to a DNA construct containing a DNA sequence which is operably linked to a suitable control sequence capable of effecting the expression of said DNA in a suitable host.
- control sequences include a promoter to effect transcription, an optional operator sequence to control such transcription, a sequence encoding suitable mRNA ribosome binding sites and sequences which control termination of transcription and translation.
- the vector may be a plasmid, a phage particle, or simply a potential genomic insert. Once transformed into a suitable host, the vector may replicate and function independently of the host genome, or may, in some instances, integrate into the genome itself.
- plasmid and vector are sometimes used interchangeably as the plasmid is the most commonly used form of vector at present. However, included herein are such other forms of expression vectors which serve equivalent functions and which are, or become, known in the art.
- the "host cells” used in the present invention generally are procaryotic or eucaryotic hosts which preferably have been manipulated by the methods disclosed in U.S. Patent 4,760,025 (RE 34,606) to render them incapable of secreting enzymatically active endoprotease.
- a preferred host cell for expressing subtilisin is the Bacillus strain BG2036 which is deficient in enzymatically active neutral protease and alkaline protease (subtilisin). The construction of strain BG2036 is described in detail in U.S. Patent 5,264,366.
- Other host cells for expressing subtilisin include Bacillus subtilis 1168 (also described in U.S.
- Patent 4,760,025 (RE 34,606) and U.S.Patent 5,264,366, the disclosure of which are incorporated herein by reference), as well as any suitable Bacillus strain such as B. licheniformis, B. lentus, etc.
- Host cells are transformed or transfected with vectors constructed using recombinant DNA techniques. Such transformed host cells are capable of either replicating vectors encoding the carbonyl hydrolase variants or expressing the desired carbonyl hydrolase variant.
- vectors which encode the pre- or prepro-form of the carbonyl hydrolase variant such variants, when expressed, are typically secreted from the host cell into the host cell medium.
- operably linked when describing the relationship between two DNA regions, simply means that they are functionally related to each other.
- a presequence is operably linked to a peptide if it functions as a signal sequence, participating in the secretion of the mature form of the protein most probably involving cleavage of the signal sequence.
- a promoter is operably linked to a coding sequence if it controls the transcription of the sequence;
- a ribosome binding site is operably linked to a coding sequence if it is positioned so as to permit translation.
- the genes encoding the naturally-occurring precursor carbonyl hydrolase may be obtained in accord with the general methods known to those skilled in the art.
- the methods generally comprise synthesizing labeled probes having putative sequences encoding regions of the hydrolase of interest, preparing genomic libraries from organisms expressing the hydrolase, and screening the libraries for the gene of interest by hybridization to the probes. Positively hybridizing clones are then mapped and sequenced.
- the B. lentus gene used in the Examples is cloned as described in Example 1 of U.S. Patent 5,185,258, the disclosure of which is incorporated herein.
- the BPN' gene used in the Examples is cloned as described in Example 1 in RE 34,606, the disclosure of which is incorporated herein.
- the cloned carbonyl hydrolase is then used to transform a host cell in order to express the hydrolase.
- the hydrolase gene is then ligated into a high copy number plasmid.
- This plasmid replicates in hosts in the sense that it contains the well-known elements necessary for plasmid replication: a promoter operably linked to the gene in question (which may be supplied as the gene's own homologous promotor if it is recognized, i.e., transcribed, by the host), a transcription termination and polyadenylation region (necessary for stability of the MRNA transcribed by the host from the hydrolase gene in certain eucaryotic host cells) which is exogenous or is supplied by the endogenous terminator region of the hydrolase gene and, desirably, a selection gene such as an antibiotic resistance gene that enables continuous cultural maintenance of plasmid-infected host cells by growth in antibiotic-containing media.
- High copy number plasmids also contain an origin of replication for the host, thereby enabling large numbers of plasmids to be generated in the cytoplasm without chromosomal limitations.
- the genes used in the present examples are a natural B. lentus gene and a natural ⁇ . amyloliquefaciens gene.
- a synthetic gene encoding a naturally-occurring or mutant precursor carbonyl hydrolase (subtilisin) may be produced.
- the DNA and/or amino acid sequence of the precursor hydrolase (subtilisin) is determined. Multiple, overlapping synthetic single-stranded DNA fragments are thereafter synthesized, which upon hybridization and ligation produce a synthetic DNA encoding the precursor hydrolase.
- An example of synthetic gene construction is set forth in Example 3 of U.S. Patent 5,204,015, the disclosure of which is incorporated herein by reference.
- cassette mutagenesis method may be used to facilitate the construction and identification of the carbonyl hydrolase variants useful in the present invention, although other methods including site-directed mutagenesis may be used.
- the naturally-occurring gene encoding the hydrolase is obtained and sequenced in whole or in part. Then the sequence is scanned for a point at which it is desired to make a mutation (deletion, insertion or substitution) of one or more amino acids in the encoded enzyme.
- the sequences flanking this point are evaluated for the presence of restriction sites for replacing a short segment of the gene with an oligonucleotide pool which when expressed will encode various mutants.
- restriction sites are preferably unique sites within the hydrolase gene so as to facilitate the replacement of the gene segment.
- any convenient restriction site which is not overly redundant in the hydrolase gene may be used, provided the gene fragments generated by restriction digestion can be reassembled in proper sequence. If restriction sites are not present at locations within a convenient distance from the selected point (from 10 to 15 nucleotides), such sites are generated by substituting nucleotides in the gene in such a fashion that neither the reading frame nor the amino acids encoded are changed in the final construction. Mutation of the gene in order to change its sequence to conform to the desired sequence is accomplished by M13 primer extension in accord with generally known methods. The task of locating suitable flanking regions and evaluating the needed changes to arrive at two convenient restriction site sequences is made routine by the redundancy of the genetic code, a restriction enzyme map of the gene and the large number of different restriction enzymes. Note that if a convenient flanking restriction site is available, the above method need be used only in connection with the flanking region which does not contain a site.
- the restriction sites flanking the positions to be mutated are digested with the cognate restriction enzymes and a plurality of end termini-complementary oligonucleotide cassettes are ligated into the gene.
- the mutagenesis is simplified by this method because all of the oligonucleotides can be synthesized so as to have the same restriction sites, and no synthetic linkers are necessary to create the restriction sites.
- proteolytic activity is defined as the rate of hydrolysis of peptide bonds per milligram of active enzyme. Many well known procedures exist for measuring proteolytic activity (K. M.
- the variant enzymes of the present invention may have other modified properties such as K m , m i ratio and/or modified substrate specificity and/or modified pH activity profile. These enzymes can be tailored for the particular substrate which is anticipated to be present, for example, for hydrolytic processes such as laundry uses.
- One objective can be to secure a variant carbonyl hydrolase having altered proteolytic activity as compared to the precursor carbonyl hydrolase, since increasing such activity (numerically larger) enables the use of the enzyme to more efficiently act on a target substrate.
- subtilisin also of interest are variant enzymes having altered thermal stability and/or altered substrate specificity as compared to the precursor.
- the carbonyl hydrolase to be mutated is a subtilisin.
- lower proteolytic activity may be desirable.
- increases or decreases (alteration) of the stability of the variant may be desirable.
- Increases or decreases in K,-,,, Konul, or K ⁇ /l ⁇ are specific to the substrate used to determine these kinetic parameters. Also, it has been determined that residues equivalent to +76 in combination with a number of other modifications in subtilisin are important in modulating overall stability and/or proteolytic activity of the enzyme.
- the Asparagine (N) in Bacillus lentus subtilisin at equivalent position +76 can be substituted with Aspartate (D) in the preferred protease enzymes in combination with modification of one or more of the following amino acid residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 to produce enhanced stability and/or enhanced activity of the resulting mutant enzyme.
- the most preferred protease enzymes useful in this invention are set forth in the Examples.
- N76D/S99D N76DA/104I
- N76D/S99D/V104I N76D/S103A/V104I
- N76D/V1041/1107V N76D/V104Y/1107V
- N76D/S101R/S103A/V104I substitutions are preferably made in Bacillus lentus (recombinant or native-type) subtilisin, although the substitutions may be made in any Bacillus subtilisin.
- Protease Manufacture Example Construction for the Expression of GG36 Gene in B. subtilis The cloning and the construction for expression of the subtilisin gene from B. lentus is performed essentially the same as that described in U.S. Patent 5,185,258.
- the plasmid GGA274 (described in Fig. 4 herein) is further modified in the following manner, as shown in Fig. 5.
- the Pstl site that is introduced during the construction of the GGA274 plasmid is removed by the oligonucleotide directed mutagenesis described below, with an oligonucleotide having the following sequence: 5' GAAGCTGCAACTCGTTAAA 3' (Seq. ID No.1 ).
- the underlined "A" residue eliminates the recognition sequence of restriction enzyme Pstl and changes the corresponding amino acid residue from alanine to threonine at position 274.
- Threonine at position 274 is the wild- type residue originally found in the cloned B. lentus subtilisin gene sequences.
- the DNA segment encoding subtilisin is excised from the plasmid GGA274 or its derivatives (GGT274 shown in Fig. 5) by EcoRI and BamHI digest. The DNA fragment is subcloned back into Bacteriophage M13-based vectors, such as MP19, for mutagenesis. After mutagenesis, the EcoRI and Hindlll digest, followed by cloning, are performed to move the mutated subtilisin gene back into an expression plasmid like GGA274 for the expression and the recovery of mutated subtilisin proteins.
- Oligonucleotide-directed mutagenesis is performed as described in
- a synthetic oligonucleotide of the sequence 5' GCTGCTCTAGACAATTCG 3' (Seq. ID No.2) is used to change the amino acid residue at position 76 from asparagine (N) to aspartic acid (D), or N76D.
- the underlined "G” and "C” residues denote changes from the wild-type gene sequence.
- the CA keeps the ieucine at position +75 and changes the amino acid sequence to introduce an Xbal recognition site of the Xbal restriction enzyme (TCTAGA), while the change at GAC changes asparagine at +76 to aspartate.
- oligonucleotides For mutagenesis at positions 99, 101, 103 and 104, different oligonucleotides can be used depending on the combination of mutations desired. For example, an oligonucleotide of the sequence 5' GTATTAGGGGCGGACGGTCGAGGCGCCATCAGCTCGATT 3'(Seq. ID No.3) is used to simultaneously make the following changes: S99D; S101 R; S103A and V104I in a single subtilisin molecule. Similarly, oligonucleotides of the sequence 5' TCAGGTTCGGTCTCGAGCGTTGCCCAAGGATTG 3' (Seq. ID No.4) and 5' CACGTTGCTAGCTTGAGTTTAG 3' (Seq.
- subtilisin variant stock solution is added to a 1 cm cuvette containing substrate dissolved in 0.1M Tris-HCL buffer, pH 8.6, and thermostated at 25°C.
- the reaction progress is followed spectrophotometrically by monitoring the absorbance of the reaction product p- nitroaniline at 410 nm.
- Kinetic parameters are obtained by using a non-linear regression algorithm to fit the reaction velocity and product concentration for each reaction to the Michaelis-Menten equation.
- Thermal Stability of Subtilisin Variants A comparison of thermal stability observed for Bacillus lentus subtilisin and the variants of the present invention made by the process of Oligonucleotide-Directed Mutagenesis hereinbefore is shown in Table IV.
- Purified enzyme 15 ug/ml in 0.1 M glycine 0.01% Tween-80 pH 10.0, with or without 50 mM CaCl2, is aliquotted into small tubes and incubated at 10°C for 5 minutes, 10°C to 60°C over 1 minute, and 60°C for 20 minutes. Tubes are then placed on ice for 10 minutes.
- variants (19 out of 26) are significantly more stable than Bacillus lentus subtilisin.
- variants N76D/S99D, N76D/V104I, N76D/S99D/V104I, N76D/S103A/V104I, N76D/V1041/1107V, N76D ⁇ 104Y/1107V and N76D/S101 R/S103A/V104I are significantly more stable than the single substitution variant N76D in the test condition without 50mM CaCI, added.
- N76D/S99D 49 98 N76D/S101R 0 82 N76D/S103A 26 92 N76D/V104I 58 98 N76D/I107V 32 96 N76D/N123S 0 97 N76D/S99D/S101R 30 100 N76D/S99D/S103A 36 100 N76D/S99D/V104I 48 97 N76D/S101R/S103A 26 100
- the mutagenesis protocol is essentially the same as described above in Oligonucleotide-Directed Mutagenesis.
- the single-stranded DNA template is generated by phagemid method.
- To construct the phagemid vector for generating the single-stranded DNA template we first construct the vector pBCDAICAT. The flow chart of vector construction is outlined in Figure 8.
- the encoding sequence of GG36DAI gene is excised as an EcoRI-BamHI fragment and cloned into the EcoRI-BamHI sites of pUCCHL5R to make pUCCAT.
- the large EcoRl-Hindlll fragment of pUCCAT is then cloned into the EcoRI and Hindlll sites of BS2KS+ to generate the plasmid pBCDAICAT.
- B. amyloliquefaciens BPN' N76D/Q103A Y104I/Y217L is done in a similar fashion except in two consecutive steps. N76D is first introduced into BPN' Y217L to make BPN' N76D/Y217L and a second mutagenesis is done to convert BPN' N76D/Y217L to BPN' N76D/Q103A/Y104I/Y217L. To generate the single-stranded DNA template for the first mutagenesis, an EcoRI-BamHI fragment encoding BPN' Y217L subtilisin (derived from the Y217L plasmid described in Wells, J., et al., PNAS.
- pUCCATFNA plasmid pUCCATFNA
- the pUCCATFNA plasmid containing BPN' Y217L is used to construct the pBCFNACAT plasmid (Fig. 9).
- Single-stranded DNA is generated as described above.
- an oligonucleotide primer with the sequence * *** ** Xbal
- BPN' N76D/Q103A/Y104I/Y217L 5' GCT GAC GGT TCC GGC GCJ AJT AGJ TGG ATC ATT 3' (Seq. ID No.15) to obtain BPN' N76D/Q103A/Y104I/Y217L. All steps involved in the cloning, the single-stranded DNA preparation, the mutagenesis, and the screening for mutants are carried out as described above. Expression of the BPN' gene and its variants are achieved by integrating plasmid DNA (pBCFNACAT containing the different variants' BPN' gene) directly into a protease-deficient strain of
- Purified enzyme is buffer-exchanged into 0.1 M glycine pH 10.0, 0.01%
- Tween-80 by applying the enzyme to a column consisting of Sephadex G-25 equilibrated with this buffer and elufmg the enzyme from the column using the same buffer.
- Half-life which is the length of time required for 50% enzyme inactivation, is determined from the first-order plot of reaction velocity as a function of the time of incubation at 60°C. The data are presented in Table VI as percent of the half-life determined or Bacillus lentus subtilisin (GG36) under identical conditions.
- K27R/N76D/V104Y/N123S/Q206L 440 1.2 3.70E+05 K27R/N76D/V104Y/N123S/S216V 440 1.2 3.70E+05 K27R/N76D ⁇ 104Y/N123S/N218S 760 0.98 7.80E+05 K27R/N76D/V104Y/N123S/T260P 410 1.2 3.40E+05 K27R/N76D/V104Y/N 123S T274A 390 1 3.90E+05 N76D/S103A/V104I/L126F/S265N 170 2.1 8.10E+04 N76D/S103A/V104I/S156E/S166D * 40 6.3 6.40E+03 K27R N76D/V104Y/N123S/G195E G1 S7E 410 0.98 4.20E+05 K27R/N76D V104Y/N123S/G195E/N218S
- BPN' amyloliquefaciens subtilisin 50 0.14 3.60E+05
- B amyloliquefaciens subtilisin 100
- the cleaning compositions of the present invention also comprise, in addition to the protease enzyme described hereinbefore, one or more cleaning composition materials compatible with the protease enzyme.
- cleaning composition materials means any liquid, solid or gaseous material selected for the particular type of cleaning composition desired and the form of the product (e.g., liquid; granule; spray composition), which materials are also compatible with the protease enzyme used in the composition.
- the specific selection of cleaning composition materials are readily made by considering the surface, item or fabric to be cleaned, and the desired form of the composition for the cleaning conditions during use (e.g., through the wash detergent use).
- compatible as used herein, means the cleaning composition materials do not reduce the proteolytic activity of the protease enzyme to such an extent that the protease is not effective as desired during normal use situations. Specific cleaning composition materials are exemplified in detail hereinafter.
- compositions useful for cleaning a variety of surfaces in need of proteinaceous stain removal include detergent compositions for cleaning hard surfaces, unlimited in form (e.g., liquid and granular); detergent compositions for cleaning fabrics, unlimited in form (e.g., granular, liquid and bar formulations); dishwashing compositions (unlimited in form); oral cleaning compositions, unlimited in form (e.g., dentifrice, toothpaste and mouthwash formulations); and denture cleaning compositions, unlimited in form (e.g., liquid, tablet).
- effective amount of protease enzyme refers to the quantity of protease enzyme described hereinbefore necessary to achieve the enzymatic activity necessary in the specific cleaning composition. Such effective amounts are readily ascertained by one of ordinary skill in the art and is based on many factors, such as the particular enzyme variant used, the cleaning application, the specific composition of the cleaning composition, and whether a liquid or dry (e.g., granular, bar) composition is required, and the like.
- the cleaning compositions of the present invention comprise from about 0.0001 % to about 10% of one or more protease enzymes, more preferably from about 0.001% to about 1%, more preferably still from about 0.001% to about 0.1%.
- protease enzymes may be employed are discussed in further detail below. All parts, percentages and ratios used herein are by weight unless otherwise specified.
- non-fabric cleaning compositions include hard surface cleaning compositions, dishwashing compositions, oral cleaning compositions, denture cleaning compositions and personal cleansing compositions.
- A. Cleaning Compositions for Hard Surfaces. Dishes and Fabrics The protease enzymes can be used in any detergent composition where high sudsing and/or good insoluble substrate removal are desired. Thus the protease enzymes can be used with various conventional ingredients to provide fully-formulated hard-surface cleaners, dishwashing compositions, fabric laundering compositions and the like. Such compositions can be in the form of liquids, granules, bars and the like. Such compositions can be formulated as modern "concentrated" detergents which contain as much as 30%-60% by weight of surfactants.
- the cleaning compositions herein can optionally, and preferably, contain various anionic, nonionic, zwitterionic, etc., surfactants.
- Such surfactants are typically present at levels of from about 0.1 % to about 60%, preferably from about 1% to about 35%, of the compositions.
- Nonlimiting examples of surfactants useful herein include the conventional Cn-Ci ⁇ alky' benzene sulfonates and primary and random alkyl sulfates, the C10-C18 secondary (2,3) alkyl sulfates of the formulas CH 3 (CH 2 )x(CHOSO3)-M + )CH 3 and CH 3 (CH 2 )y(CHOS0 3 -M + ) CH 2 CH 3 wherein x and (y+1 ) are integers of at least about 7, preferably at least about 9, and M is a water-solubilizing cation, especially sodium, the C10-C18 alk y' alkoxy sulfates (especially EO 1-7 ethoxy sulfates), C10-C18 alkyl alkoxy carboxylates (especially the EO 1-7 ethoxycarboxylates), the C-jo-C ⁇ alkyl polyglycosides, and their corresponding sulfated polyglycosides, C
- alkyl alkoxy sulfates AES
- alkyl alkoxy carboxylates AEC
- AES alkyl alkoxy sulfates
- AEC alkyl alkoxy carboxylates
- Other conventional useful surfactants are listed in standard texts. Particularly useful surfactants include the C-JQ-CIS N-methyl glucamides disclosed in US Patent 5, 194,639, Connor et al., issued March 16, 1993, inco ⁇ orated herein by reference.
- nonionic surfactants which are condensates of ethylene oxide with a hydrophobic moiety to provide a surfactant having an average hydrophilic-lipophilic balance (HLB) in the range from 5 to 17, preferably from 6 to 14, more preferably from 7 to 12.
- HLB hydrophilic-lipophilic balance
- the hydrophobic (lipophilic) moiety may be aliphatic or aromatic in nature and the length of the polyoxyethylene group which is condensed with any particular hydrophobic group can be readily adjusted to yield a water-soluble compound having the desired degree of balance between hydrophilic and hydrophobic elements.
- the C9-C15 primary alcohol ethoxylates (or mixed ethoxy/propoxy) containing 3-8 moles of ethylene oxide per mole of alcohol, particularly the C14-C15 primary alcohols containing 6-8 moles of ethylene oxide per mole of alcohol, the C12-C15 primary alcohols containing 3- 5 moles of ethylene oxide per mole of alcohol, and mixtures thereof.
- suds boosters such as the C10-C16 alkolamides can be incorporated into the compositions, typically at about 1% to about 10% levels.
- the C10-C1 monoethanol and diethanol amides illustrate a typical class of such suds boosters.
- Use of such suds boosters with high sudsing adjunct surfactants such as the amine oxides, betaines and sultaines noted above is also advantageous.
- soluble magnesium salts such as MgCl2, MgSO.4, and the like, can be added at levels of, typically, from about 0.1% to about 2%, to provide additional sudsing.
- the liquid detergent compositions herein can contain water and other solvents as carriers.
- Low molecular weight primary or secondary alcohols exemplified by methanol, ethanol, propanol, and isopropanol are suitable.
- Monohydric alcohols are preferred for solubilizing surfactants, but polyols such as those containing from about 2 to about 6 carbon atoms and from about 2 to about 6 hydroxy groups (e.g., 1,3-propanediol, ethylene glycol, glycerine, and 1 ,2-propanediol) can also be used.
- the compositions may contain from about 5% to about 90%, typically from about 10% to about 50% of such carriers.
- the detergent compositions herein will preferably be formulated such that during use in aqueous cleaning operations, the wash water will have a pH between about 6.8 and about 11.0. Finished products thus are typically formulated at this range. Techniques for controlling pH at recommended usage levels include the use of buffers, alkalis, acids, etc., and are well known to those skilled in the art.
- the formulator may wish to employ various builders at levels from about 5% to about 50% by weight.
- Typical builders include the 1-10 micron zeolites, polycarboxylates such as citrate and oxydisuccinates, layered silicates, phosphates, and the like.
- Other conventional builders are listed in standard formularies.
- the formulator may wish to employ various additional enzymes, such as cellulases, lipases, amylases, peroxidases, and proteases in such compositions, typically at levels of from about 0.001 % to about 1 % by weight.
- additional enzymes such as cellulases, lipases, amylases, peroxidases, and proteases
- cellulases such as cellulases, lipases, amylases, peroxidases, and proteases
- bleaching compounds such as the percarbonates, perborates and the like, can be used in such compositions, typically at levels from about 1 % to about 15% by weight.
- such compositions can also contain bleach activators such as tetraacetyl ethylenediamine, nonanoyloxybenzene sulfonate, and the like, which are also known in the art. Usage levels typically range from about 1% to about 10% by weight.
- Various soil release agents especially of the anionic oligoester type, various chelating agents, especially the aminophosphonates and ethylenediaminedisuccinates, various clay soil removal agents, especially ethoxylated tetraethylene pentamine, various dispersing agents, especially polyacrylates and polyasparatates, various brighteners, especially anionic brighteners, various dye transfer inhibiting agents, such as polyvinyl pyrrolidone, various suds suppressors, especially silicones and secondary alcohols, various fabric softeners, especially smectite clays and clay floculating polymers (e.g., poly(oxy ethylene)), and the like can all be used in such compositions at levels ranging from about 1 % to about 35% by weight. Standard formularies and published patents contain multiple, detailed descriptions of such conventional materials.
- Enzyme stabilizers may also be used in the cleaning compositions of the present invention.
- Such enzyme stabilizers include propylene glycol
- Hard surface cleaning compositions refers to liquid and granular detergent compositions for cleaning hard surfaces such as floors, walls, bathroom tile, and the like. Hard surface cleaning compositions of the present invention comprise an effective amount of one or more protease enzymes, preferably from about 0.0001% to about 10%, more preferably from about 0.001 % to about 5%, more preferably still from about 0.001 % to about 1 % by weight of active protease enzyme of the composition.
- such hard surface cleaning compositions typically comprise a surfactant and a water-soluble sequestering builder.
- the surfactants are sometimes not used since they may produce a filmy/streaky residue on the glass surface.
- the surfactant component when present, may comprise as little as 0.1% of the compositions herein, but typically the compositions will contain from about 0.25% to about 10%, more preferably from about 1% to about 5% of surfactant.
- compositions will contain from about 0.5% to about 50% of a detergency builder, preferably from about 1 % to about 10%.
- pH should be in the range of about 8 to 12.
- Conventional pH adjustment agents such as sodium hydroxide, sodium carbonate or hydrochloric acid can be used if adjustment is necessary.
- Solvents may be included in the compositions.
- Useful solvents include, but are not limited to, glycol ethers such as diethyleneglycol monohexyl ether, diethyleneglycol monobutyl ether, ethyleneglycol monobutyl ether, ethyleneglycol monohexyl ether, propyleneglycol monobutyl ether, dipropyleneglycol monobutyl ether, and diols such as 2,2,4-trimethyl-1,3- pentanediol and 2-ethyl-1 ,3-hexanediol. When used, such solvents are typically present at levels of from about 0.5% to about 15%, preferably from about 3% to about 11 %.
- volatile solvents such as isopropanol or ethanol can be used in the present compositions to facilitate faster evaporation of the composition from surfaces when the surface is not rinsed after "full strength" application of the composition to the surface.
- volatile solvents are typically present at levels of from about 2% to about 12% in the compositions.
- the hard surface cleaning composition embodiment of the present invention is illustrated by the following nonlimiting examples. (In the following examples, reference to “Protease #" in the examples is to the variant useful in the present invention compositions having the given number in Table III hereinbefore.)
- dishwashing compositions comprise one or more protease enzymes.
- dishwashing composition refers to all forms for compositions for cleaning dishes, including but not limited to, granular and liquid forms.
- the dishwashing composition embodiment of the present invention is illustrated by the following examples. Examples 13-18
- Mg ++ (as MgCl2) 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20
- Example 19 Granular Automatic Dishwashing Composition Component A B C Citric Acid 15.0 - - Citrate 4.0 29.0 15.0
- Example 19 A-C the Protease #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention described in Tables V and VI, are substituted for Protease #12, with substantially similar results. Also in Examples 19 A-C, any combination of the proteases useful in the present invention recited in Tables III, V and VI among others, are substituted for Protease # 12 with substantially similar results. 3. Fabric cleaning compositions
- fabric cleaning compositions comprise one or more protease enzymes.
- fabric cleaning composition refers to all forms for detergent compositions for cleaning fabrics, including but not limited to, granular, liquid and bar forms. a. Granular fabric cleaning compositions
- the granular fabric cleaning compositions of the present invention contain an effective amount of one or more protease enzymes, preferably from about 0.001% to about 10%, more preferably from about 0.005% to about 5%, more preferably from about 0.01% to about 1% by weight of active protease enzyme of the composition.
- the granular fabric cleaning compositions typically comprise at least one surfactant, one or more builders, and, in some cases, a bleaching agent.
- the granular fabric cleaning composition embodiment of the present invention is illustrated by the following examples.
- Zeolite A (1-10 mi ⁇ ometer) 26.00 26.00 26.00 26.00 26.00
- Fillers e.g., silicates; carbonates; perfumes; Up to 100 Up to 100 water
- Liquid fabric cleaning compositions of the present invention comprise an effective amount of one or more protease enzymes, preferably from about 0.0001% to about 10%, more preferably from about 0.001% to about 1%, and most preferably from about 0.001% to about 0.1%, by weight of active protease enzyme of the composition.
- Such liquid fabric cleaning compositions typically additionally comprise an anionic surfactant, a fatty acid, a water-soluble detergency builder and water.
- liquid fabric cleaning composition embodiment of the present invention is illustrated by the following examples.
- Ci2- C14 alkyl sulfate Na 20.00 20.00 20.00 20.00 20.00 20.00
- Citric acid monohydrate 10.0 15.0
- Ci2-15 alcohol 7 times ethoxylated - 8.0
- Bar fabric cleaning compositions of the present invention suitable for hand-washing soiled fabrics contain an effective amount of one or more protease enzymes, preferably from about 0.001% to about 10%, more preferably from about 0.01 % to about 1 % by weight of the composition.
- the bar fabric cleaning composition embodiment of the present invention is illustrated by the following examples. Examples 42-45
- Zeolite A (0.1 -.10 ⁇ ) 5.0 5.0 5.0 5.00
- Polya ⁇ ylate (MW 1400) 0.2 0.2 0.2 0.20
- one or more protease enzymes may be inco ⁇ orated into a variety of other cleaning compositions where hydrolysis of an insoluble substrate is desired.
- additional cleaning compositions include but are not limited to, oral cleaning compositions, denture cleaning compositions, and contact lens cleaning compositions. 1. Oral cleaning compositions
- oral cleaning compositions refers to dentifrices, toothpastes, toothgels, toothpowders, mouthwashes, mouth sprays, mouth gels, chewing gums, lozenges, sachets, tablets, biogels, prophylaxis pastes, dental treatment solutions, and the like.
- oral cleaning compositions of the present invention comprise from about 0.0001% to about 20% of one or more protease enzymes, more preferably from about 0.001% to about 10%, more preferably still from about 0.01% to about 5%, by weight of the composition, and a pharmaceutical ly-acceptable carrier.
- pharmaceutical ly- acceptable means that drugs, medicaments or inert ingredients which the term describes are suitable for use in contact with the tissues of humans and lower animals without undue toxicity, incompatibility, instability, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio.
- the pharmaceutically-acceptable oral cleaning carrier components of the oral cleaning components of the oral cleaning compositions will generally comprise from about 50% to about 99.99%, preferably from about 65% to about 99.99%, more preferably from about 65% to about 99%, by weight of the composition.
- compositions of the present invention are well known to those skilled in the art.
- a wide variety of composition types, carrier components and optional components useful in the oral cleaning compositions are disclosed in U.S. Patent 5,096,700, Seibel, issued March 17, 1992; U.S. Patent 5,028,414, Sampathkumar, issued July 2, 1991; and U.S. Patent 5,028,415, Benedict, Bush and Sunberg, issued July 2, 1991; all of which are inco ⁇ orated herein by reference.
- the oral cleaning composition embodiment of the present invention is illustrated by the following examples. Examples 46-49 Dentifrice Composition
- Carboxyvinyl Polymer *** 0.300 0.300 0.300 0.300 Carrageenan* 0.800 0.800 0.800 0.800 Water balance to 100%
- *PEG-6 Polyethylene glycol having a molecular weight of 600. * * Precipitated silica identified as Zeodent 119 offered by J.M. Huber. ***Carbopol offered by B.F. Goodrich Chemical Company. * *** lota Carrageenan offered by Hercules Chemical Company.
- Examples 46-49 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 46-49, any combination of the protease enzymes useful in the present invention recited in Tables III, V, VI, among others, are substituted for Protease # 12 with substantially similar results. Examples 50-53
- Examples 50-53 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 50-53, any combination of the protease enzymes useful in the present invention recited in Tables III, V, and VI, among others, are substituted for Protease # 12 with substantially similar results.
- Examples 54-57 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 54-57, any combination of the protease enzymes useful in the present invention recited in Tables III, V and VI, among others, are substituted for Protease # 12 with substantially similar results.
- Examples 58-61 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 58-61 , any combination of the protease enzymes useful in the present invention recited in Tables III, V, and VI, among others, are substituted for Protease # 12 with substantially similar results. 2. Denture cleaning compositions
- denture cleaning compositions for cleaning dentures outside of the oral cavity comprise one or more protease enzymes.
- Such denture cleaning compositions comprise an effective amount of one or more protease enzymes, preferably from about 0.0001 % to about 50% of one or more protease enzymes, more preferably from about 0.001% to about 35%, more preferably still from about 0.01% to about 20%, by weight of the composition, and a denture cleansing carrier.
- denture cleansing composition formats such as effervescent tablets and the like are well known in the art (see for example U.S.
- Patent 5,055,305, Young, inco ⁇ orated herein by reference are generally appropriate for inco ⁇ oration of one or more protease enzymes for removing proteinaceous stains from dentures.
- the denture cleaning composition embodiment of the present invention is illustrated by the following examples.
- Flavor 1.0 1.0 1.00 1.00
- Examples 62-65 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 62-65, any combination of the protease enzymes useful in the present invention recited in Tables III, V and VI, among others, are substituted for Protease # 12 with substantially similar results. 3.
- personal Cleansing Compositions In another embodiment of the present invention, personal cleaning compositions for cleaning the skin (in liquid and bar form) comprise one or more of the protease enzymes.
- compositions typically comprise from about 0.001% to about 5% protease enzyme, preferably from about 0.005% to about 2%, and most preferably from about 0.01 % to about 0.8% by weight of * the composition.
- Preferred personal cleansing compositions into which can be included protease enzymes as described herein are taught in U.S. Patent Applications SN 08/121,623 and SN 08/121,624, both by Visscher et al., filed September 14, 1993, the disclosures of which are incorporated herein by reference in their entirety. Such compositions are illustrated by the following examples.
- Examples 66-67 the Proteases #'s 1-11 and 13-25 recited in Table III, among others including the additional proteases useful in the present invention des ⁇ ibed in Tables V and VI, are substituted for Protease # 12, with substantially similar results. Also in Examples 66-67, any combination of the protease enzymes useful in the present invention recited in Tables III, V, and VI, among others, are substituted for Protease # 12 with substantially similar results.
- Example 68 Wash Performance Test The wash performance of the variants useful in the present invention compositions is evaluated by measuring the removal of stain from EMPA 116 (blood/milk/carbon black on cotton) cloth swatches (Testfabrics, Inc., Middlesex, NJ 07030).
- EMPA 116 swatches cut to 3 X 4-1/2 inches with pinked edges, are placed in each pot of a Model 7243S Terg-O-Tometer (United States Testing Co., Inc., Hoboken, NJ) containing 1000 ml of water, 15 gpg hardness (Ca ++ :Mg ++ ::3:1::w:w), 7 g of detergent, and enzyme as appropriate.
- the detergent base is WFK1 detergent from wfc - Testgewebe GmbH, Adierstrasse 42, Postfach 130762, D-47759 Krefeld, Germany:
- Sodium perborate monohydrate is obtained from Degussa Corporation,
- Sokalan CP5 is obtained from BASF Corporation, Parsippany, NJ 07054.
- Mykon ATC Green (TAED, tetraacetylethylenediamine) is obtained from Warwick International, Limited, Mostyn, Holywell, Clwyd CH8 9HE, England.
- Tinopal AMS GX is obtained from Ciba-Geigy Corporation, Greensboro, NC 27419.
- Example 69 Protease Stability in a Liouid Detergent Formulation
- a comparison of protease stability toward inactivation in a liquid detergent formulation is made for Bacillus lentus subtilisin and it's variant enzyme N76D/S103A/V104I according to the procedure outlined herein.
- the detergent formulation for the study is a commercially purchased bottle of Tide Ultra liquid lanudry detergent made in the USA by The Procter & Gamble Company. Heat treatment of the detergent formulation is necessary to inactivate in-situ protease. This is accomplished by incubating the detergent at 96°C for a period of 4.5 hours. Concentrated preparations of the B.
- lentus subtilisin and N76D/S103A/V104I variant in the range of 20 grams/liter enzyme, are then added to the heat-treated Tide Ultra at room-temperature to a final concentratrion of 0.3 grams/liter enzyme in the detergent formulation.
- the heat-treated detergent with protease added is then incubated in a water bath thermostatted at 50°C. Aliquots are removed from the incubation tubes at 0, 24, 46, 76, and 112 hour time intervals and assayed for enzyme activity by addition to a 1 cm cuvette containing 1.2 mM of the synthetic peptide substrate suc-Ala-Ala-Pro-phe-p-nitroanilide dissolved in 0.1 M tris-HCL buffer, pH 8.6, and thermostatted at 25°C.
- the initial linear reaction velocity is followed spectrophotometrically by monitoring the absorbance of the reaction product p- nitroaniline at 410nm as a function of time.
- the N76D/S103A/V104I variant is observed to have significantly greater stability towards inactivation than the native B. lentus enzyme.
- Estimated half-lives for inactivation in the Tide Ultra detergent formulation for the two enzymes, under the specified test conditions, are 45 hours for B. lentus subtilisin and 125 hours for the N76D/S103A/V104I variant.
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
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DE69434962T DE69434962T2 (en) | 1993-10-14 | 1994-10-13 | PROTEASE-CONTAINING DETERGENTS |
JP7512131A JP2888985B2 (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning composition |
AU80797/94A AU8079794A (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning compositions |
EP94931872A EP0723579B1 (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning compositions |
HU9600960A HU219851B (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning compositions |
BR9407834A BR9407834A (en) | 1993-10-14 | 1994-10-13 | Cleaning compositions containing protease |
CA002173105A CA2173105C (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning compositions |
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US13679793A | 1993-10-14 | 1993-10-14 | |
US08/136,797 | 1993-10-14 | ||
US23793894A | 1994-05-02 | 1994-05-02 | |
US08/237,938 | 1994-05-02 |
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WO1995010591A1 true WO1995010591A1 (en) | 1995-04-20 |
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PCT/US1994/011776 WO1995010591A1 (en) | 1993-10-14 | 1994-10-13 | Protease-containing cleaning compositions |
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US (2) | US5679630A (en) |
EP (1) | EP0723579B1 (en) |
JP (2) | JP2888985B2 (en) |
CN (1) | CN1082999C (en) |
AT (1) | ATE361355T1 (en) |
AU (1) | AU8079794A (en) |
BR (1) | BR9407834A (en) |
CA (1) | CA2173105C (en) |
CZ (1) | CZ105396A3 (en) |
DE (1) | DE69434962T2 (en) |
ES (1) | ES2287931T3 (en) |
HU (1) | HU219851B (en) |
TW (1) | TW306931B (en) |
WO (1) | WO1995010591A1 (en) |
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---|---|---|---|---|
WO1996016154A1 (en) * | 1994-11-18 | 1996-05-30 | The Procter & Gamble Company | Detergent compositions containing lipase and protease |
WO1997007770A1 (en) * | 1995-08-23 | 1997-03-06 | Henkel Kommanditgesellschaft Auf Aktien | Use of mutated subtilisin protease in cosmetic products |
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WO2001075087A2 (en) | 2000-04-03 | 2001-10-11 | Maxygen, Inc. | Subtilisin variants |
WO2001068821A3 (en) * | 2000-03-14 | 2002-04-04 | Novozymes As | Novel subtilase enzymes having an improved wash performance on egg stains |
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US7153820B2 (en) | 2001-08-13 | 2006-12-26 | Ecolab Inc. | Solid detergent composition and method for solidifying a detergent composition |
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Families Citing this family (399)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK39093D0 (en) * | 1993-04-01 | 1993-04-01 | Novo Nordisk As | ENZYME |
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
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IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
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AR007837A1 (en) * | 1996-07-08 | 1999-11-24 | Procter & Gamble | DETERGENT COMPOSITION FOR WASHING INCLUDING A COMBINATION OF SURFACTANTS |
US6313086B1 (en) * | 1996-07-31 | 2001-11-06 | The Procter & Gamble Company | Detergent compositions containing and effervescent |
CA2268618A1 (en) * | 1996-10-18 | 1998-04-30 | Robin Gibson Hall | Detergent compositions comprising a mixture of cationic, anionic and nonionic surfactants |
EP0913458B1 (en) * | 1997-10-22 | 2004-06-16 | The Procter & Gamble Company | Liquid hard-surface cleaning compositions |
ES2367505T3 (en) * | 1997-10-23 | 2011-11-04 | Danisco Us Inc. | PROTEASE VARIANTS WITH MULTIPLE SUBSTITUTIONS WITH ALTERED NET LOAD FOR USE IN DETERGENTS. |
AU2742499A (en) * | 1998-03-26 | 1999-10-18 | Procter & Gamble Company, The | Serine protease variants having amino acid substitutions |
US6420331B1 (en) | 1998-06-10 | 2002-07-16 | Procter & Gamble Company | Detergent compositions comprising a mannanase and a bleach system |
US6376450B1 (en) * | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
DE19942595C2 (en) * | 1998-11-18 | 2002-01-31 | Linders Werner | Denture cleaners |
US7241729B2 (en) * | 1999-05-26 | 2007-07-10 | Rhodia Inc. | Compositions and methods for using polymeric suds enhancers |
EP1194461B1 (en) * | 1999-05-26 | 2008-10-08 | Rhodia Inc. | Block polymers, compositions and methods of use for foams, laundry detergents, shower rinses and coagulants |
US7939601B1 (en) | 1999-05-26 | 2011-05-10 | Rhodia Inc. | Polymers, compositions and methods of use for foams, laundry detergents, shower rinses, and coagulants |
US20050124738A1 (en) * | 1999-05-26 | 2005-06-09 | The Procter & Gamble Company | Compositions and methods for using zwitterionic polymeric suds enhancers |
AU6260299A (en) | 1999-09-22 | 2001-04-24 | Procter & Gamble Company, The | A hand-held liquid container |
US20020068014A1 (en) * | 2000-02-18 | 2002-06-06 | Haught John Christian | Antibacterial agents and compositions, methods and systems employing same |
AU2001239038A1 (en) * | 2000-02-29 | 2001-09-12 | Clariant S.A. | Cosmetic compositions comprising anionic and cationic surfactants |
US7319112B2 (en) * | 2000-07-14 | 2008-01-15 | The Procter & Gamble Co. | Non-halogenated antibacterial agents and processes for making same |
US7109016B2 (en) | 2000-08-21 | 2006-09-19 | Novozymes A/S | Subtilase enzymes |
AU7961401A (en) * | 2000-08-21 | 2002-03-04 | Novozymes As | Subtilase enzymes |
US6541234B1 (en) * | 2000-09-11 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
GB2367065B (en) * | 2000-09-20 | 2002-11-20 | Reckitt Benckiser Inc | Enzyme containing laundry booster compositions |
US6541235B1 (en) * | 2000-09-29 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
DE10104479C1 (en) * | 2001-01-31 | 2002-09-12 | Gerhard Knapp | Combination of agents used for inhibiting mite droppings, e.g. for spraying mattress or bed, consists of auxilase, water, anionic surfactant, fragrance and preservative |
US20030040459A1 (en) * | 2001-02-05 | 2003-02-27 | Unilever Home & Pesonal Care Usa | Cleaning compositions |
CN100489094C (en) * | 2001-03-23 | 2009-05-20 | 金克克国际有限公司 | Proteins with altered immunogenicity and methods of making and using the same |
DE10121463A1 (en) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants |
US7157416B2 (en) * | 2001-07-20 | 2007-01-02 | Genencor International, Inc. | Stabilization of enzymes |
GB0118932D0 (en) * | 2001-08-02 | 2001-09-26 | Unilever Plc | Improvements relating to laundry compositions |
US6933269B2 (en) * | 2001-08-03 | 2005-08-23 | The Procter & Gamble Company | Polyaspartate derivatives for use in detergent compositions |
US6544941B1 (en) * | 2001-08-27 | 2003-04-08 | Unilever Home & Personal Care Usa, Division Of Conopco, Inc. | Dishwashing composition |
DE10153792A1 (en) * | 2001-10-31 | 2003-05-22 | Henkel Kgaa | New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants |
WO2003062380A2 (en) * | 2002-01-16 | 2003-07-31 | Genencor International, Inc. | Multiply-substituted protease variants |
EP1523553B1 (en) * | 2002-01-16 | 2009-12-02 | Genencor International, Inc. | Multiply-substituted protease variants |
EP1542535A1 (en) | 2002-08-28 | 2005-06-22 | KAPITZ, Carl-Heinz | Use of proteases for decomposing allergens |
WO2004033668A2 (en) * | 2002-10-10 | 2004-04-22 | Diversa Corporation | Proteases, nucleic acids encoding them and methods for making and using them |
BR0303954A (en) | 2002-10-10 | 2004-09-08 | Int Flavors & Fragrances Inc | Composition, fragrance, method for dividing an olfactory effective amount of fragrance into a non-rinse and non-rinse product |
US7585824B2 (en) | 2002-10-10 | 2009-09-08 | International Flavors & Fragrances Inc. | Encapsulated fragrance chemicals |
US7402554B2 (en) * | 2003-02-28 | 2008-07-22 | The Procter & Gamble Company | Foam-generating kit containing a foam-generating dispenser and a composition containing a high level of surfactant |
US20040229763A1 (en) * | 2003-02-28 | 2004-11-18 | The Procter & Gamble Company | Cleaning kit and/or a dishwashing kit containing a foam-generating dispenser and a cleaning and/or dishwashing composition |
US7651992B2 (en) * | 2003-02-28 | 2010-01-26 | The Procter & Gamble Company | Foam-generating kit containing a foam-generating dispenser and a composition containing a high level of surfactant |
EP1623029B1 (en) * | 2003-05-07 | 2008-09-03 | Novozymes A/S | Variant subtilisin enzymes (subtilases) |
US20050112152A1 (en) | 2003-11-20 | 2005-05-26 | Popplewell Lewis M. | Encapsulated materials |
US7105064B2 (en) | 2003-11-20 | 2006-09-12 | International Flavors & Fragrances Inc. | Particulate fragrance deposition on surfaces and malodour elimination from surfaces |
US20050113282A1 (en) * | 2003-11-20 | 2005-05-26 | Parekh Prabodh P. | Melamine-formaldehyde microcapsule slurries for fabric article freshening |
US20050226900A1 (en) * | 2004-04-13 | 2005-10-13 | Winton Brooks Clint D | Skin and hair treatment composition and process for using same resulting in controllably-releasable fragrance and/or malodour counteractant evolution |
US20050227907A1 (en) * | 2004-04-13 | 2005-10-13 | Kaiping Lee | Stable fragrance microcapsule suspension and process for using same |
US7419943B2 (en) | 2004-08-20 | 2008-09-02 | International Flavors & Fragrances Inc. | Methanoazuenofurans and methanoazulenone compounds and uses of these compounds as fragrance materials |
US7594594B2 (en) * | 2004-11-17 | 2009-09-29 | International Flavors & Fragrances Inc. | Multi-compartment storage and delivery containers and delivery system for microencapsulated fragrances |
US7871972B2 (en) | 2005-01-12 | 2011-01-18 | Amcol International Corporation | Compositions containing benefit agents pre-emulsified using colloidal cationic particles |
WO2006076454A1 (en) | 2005-01-12 | 2006-07-20 | Amcol International Corporation | Detersive compositions containing hydrophobic benefit agents pre-emulsified using colloidal cationic particles |
US7977288B2 (en) | 2005-01-12 | 2011-07-12 | Amcol International Corporation | Compositions containing cationically surface-modified microparticulate carrier for benefit agents |
US20070207174A1 (en) * | 2005-05-06 | 2007-09-06 | Pluyter Johan G L | Encapsulated fragrance materials and methods for making same |
CA2620738C (en) * | 2005-08-31 | 2010-10-12 | Ezaki Glico Co., Ltd. | Tablet for removing tongue coating |
DK2390321T3 (en) * | 2005-10-12 | 2015-02-23 | Procter & Gamble | The use and manufacture of a storage stable neutral metalloprotease |
US20070138674A1 (en) | 2005-12-15 | 2007-06-21 | Theodore James Anastasiou | Encapsulated active material with reduced formaldehyde potential |
US20070138673A1 (en) | 2005-12-15 | 2007-06-21 | Kaiping Lee | Process for Preparing a High Stability Microcapsule Product and Method for Using Same |
US7838481B2 (en) * | 2006-04-07 | 2010-11-23 | Beckman Coulter, Inc. | Formaldehyde-free cleaner composition for cleaning blood analyzers and method of use |
US7629158B2 (en) * | 2006-06-16 | 2009-12-08 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
CN104232365A (en) * | 2006-07-18 | 2014-12-24 | 丹尼斯科美国公司 | Protease variants active over a broad temperature range |
US20080027505A1 (en) * | 2006-07-26 | 2008-01-31 | G&L Consulting, Llc | System and method for treatment of headaches |
US7833960B2 (en) | 2006-12-15 | 2010-11-16 | International Flavors & Fragrances Inc. | Encapsulated active material containing nanoscaled material |
US7709436B2 (en) * | 2007-05-09 | 2010-05-04 | The Dial Corporation | Low carbon footprint compositions for use in laundry applications |
US7648953B2 (en) * | 2008-05-08 | 2010-01-19 | The Dial Corporation | Eco-friendly laundry detergent compositions comprising natural essence |
EP2164448A4 (en) | 2007-05-14 | 2012-07-04 | Amcol International Corp | Compositions containing benefit agent composites pre-emulsified using colloidal cationic particles |
US20080311064A1 (en) * | 2007-06-12 | 2008-12-18 | Yabin Lei | Higher Performance Capsule Particles |
ES1065735Y (en) * | 2007-06-19 | 2008-01-16 | Sensient Fragances S A | SOAP PAD WITH ACTIVE NUCLEUS |
US7998920B2 (en) * | 2008-01-22 | 2011-08-16 | Stepan Company | Sulfonated estolide compositions containing magnesium sulfate and processes employing them |
US7879790B2 (en) * | 2008-01-22 | 2011-02-01 | Stepan Company | Mixed salts of sulfonated estolides and other derivatives of fatty acids, and methods of making them |
US7666828B2 (en) | 2008-01-22 | 2010-02-23 | Stepan Company | Sulfonated estolides and other derivatives of fatty acids, methods of making them, and compositions and processes employing them |
US8066818B2 (en) | 2008-02-08 | 2011-11-29 | The Procter & Gamble Company | Water-soluble pouch |
PL2088187T3 (en) * | 2008-02-08 | 2013-01-31 | Procter & Gamble | Process For Making A Water-Soluble Pouch |
EP2265702A1 (en) | 2008-02-08 | 2010-12-29 | Amcol International Corporation | Compositions containing cationically surface-modified microparticulate carrier for benefit agents |
US8236747B2 (en) * | 2008-02-08 | 2012-08-07 | Method Products, Inc. | Consumer product packets with enhanced performance |
US20090209447A1 (en) * | 2008-02-15 | 2009-08-20 | Michelle Meek | Cleaning compositions |
US20090233830A1 (en) * | 2008-03-14 | 2009-09-17 | Penny Sue Dirr | Automatic detergent dishwashing composition |
EP2100948A1 (en) * | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
US8097047B2 (en) * | 2008-04-02 | 2012-01-17 | The Procter & Gamble Company | Fabric color rejuvenation composition |
US8188022B2 (en) | 2008-04-11 | 2012-05-29 | Amcol International Corporation | Multilayer fragrance encapsulation comprising kappa carrageenan |
WO2009126960A2 (en) | 2008-04-11 | 2009-10-15 | Amcol International Corporation | Multilayer fragrance encapsulation |
JP5647976B2 (en) | 2008-06-06 | 2015-01-07 | ダニスコ・ユーエス・インク | Compositions and methods comprising mutant microbial proteases |
EP2166092A1 (en) | 2008-09-18 | 2010-03-24 | The Procter and Gamble Company | Detergent composition |
EP2166076A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
EP2166073A1 (en) | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
EP2166075A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter and Gamble Company | Cleaning composition |
US7915215B2 (en) | 2008-10-17 | 2011-03-29 | Appleton Papers Inc. | Fragrance-delivery composition comprising boron and persulfate ion-crosslinked polyvinyl alcohol microcapsules and method of use thereof |
EP2362902B1 (en) * | 2008-11-11 | 2012-10-24 | Danisco US, Inc., Genencor Division | Compositions and methods comprising a subtilisin variant |
US20100267304A1 (en) * | 2008-11-14 | 2010-10-21 | Gregory Fowler | Polyurethane foam pad and methods of making and using same |
US20100125046A1 (en) | 2008-11-20 | 2010-05-20 | Denome Frank William | Cleaning products |
US8058223B2 (en) * | 2009-01-21 | 2011-11-15 | Stepan Company | Automatic or machine dishwashing compositions of sulfonated estolides and other derivatives of fatty acids and uses thereof |
US8119588B2 (en) * | 2009-01-21 | 2012-02-21 | Stepan Company | Hard surface cleaner compositions of sulfonated estolides and other derivatives of fatty acids and uses thereof |
US7884064B2 (en) * | 2009-01-21 | 2011-02-08 | Stepan Company | Light duty liquid detergent compositions of sulfonated estolides and other derivatives of fatty acids |
US8124577B2 (en) * | 2009-01-21 | 2012-02-28 | Stepan Company | Personal care compositions of sulfonated estolides and other derivatives of fatty acids and uses thereof |
BRPI0924129B1 (en) | 2009-01-21 | 2019-09-17 | Stepan Company | AGRICULTURAL COMPOSITION OF SULFONED STOLIDES AND OTHER FATTY ACID DERIVATIVES |
ES2488117T3 (en) | 2009-02-02 | 2014-08-26 | The Procter & Gamble Company | Liquid detergent composition for dishwashing by hand |
EP2213715A1 (en) | 2009-02-02 | 2010-08-04 | The Procter & Gamble Company | Liquid hand dishwashing detergent composition |
EP2216390B1 (en) | 2009-02-02 | 2013-11-27 | The Procter and Gamble Company | Hand dishwashing method |
EP2216392B1 (en) | 2009-02-02 | 2013-11-13 | The Procter and Gamble Company | Liquid hand dishwashing detergent composition |
ES2461892T3 (en) | 2009-02-02 | 2014-05-21 | The Procter & Gamble Company | Liquid detergent composition for dishwashing by hand |
EP2216391A1 (en) | 2009-02-02 | 2010-08-11 | The Procter & Gamble Company | Liquid hand dishwashing detergent composition |
EP3998328A1 (en) | 2009-02-09 | 2022-05-18 | The Procter & Gamble Company | Detergent composition |
WO2011005917A1 (en) * | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
EP2451922A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
EP2451919A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
MX2012000486A (en) | 2009-07-09 | 2012-01-27 | Procter & Gamble | A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte. |
WO2011005730A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte |
US20110005004A1 (en) * | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
MX342487B (en) | 2009-07-09 | 2016-09-29 | The Procter & Gamble Company * | A mildly alkaline, low-built, solid fabric treatment detergent composition comprising phthalimido peroxy caproic acid. |
EP2277860B1 (en) | 2009-07-22 | 2015-08-19 | Stepan Company | Compositions comprising sulfonated estolides and alkyl ester sulfonates, methods of making them, and compositions and processes employing them |
PL2292725T5 (en) | 2009-08-13 | 2022-11-07 | The Procter And Gamble Company | Method of laundering fabrics at low temperature |
DE102009029513A1 (en) * | 2009-09-16 | 2011-03-24 | Henkel Ag & Co. Kgaa | Storage-stable liquid washing or cleaning agent containing proteases |
CN102120167B (en) | 2009-09-18 | 2014-10-29 | 国际香料和香精公司 | encapsulated active material |
EP2322595A1 (en) | 2009-11-12 | 2011-05-18 | The Procter & Gamble Company | Solid laundry detergent composition |
EP2322593A1 (en) | 2009-11-12 | 2011-05-18 | The Procter & Gamble Company | Liquid laundry detergent composition |
EP2510092A1 (en) * | 2009-12-09 | 2012-10-17 | The Procter & Gamble Company | Fabric and home care products |
EP2333040B2 (en) | 2009-12-10 | 2019-11-13 | The Procter & Gamble Company | Detergent composition |
ES2423580T5 (en) | 2009-12-10 | 2021-06-17 | Procter & Gamble | Method and use of a dishwashing composition |
EP2333042B1 (en) | 2009-12-10 | 2015-07-01 | The Procter and Gamble Company | Automatic dishwashing product and use thereof |
EP2333041B1 (en) | 2009-12-10 | 2013-05-15 | The Procter & Gamble Company | Method and use of a dishwasher composition |
MX369096B (en) | 2010-02-10 | 2019-10-29 | Novozymes As | Variants and compositions comprising variants with high stability in presence of a chelating agent. |
EP2357220A1 (en) | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
PL2361964T3 (en) | 2010-02-25 | 2013-05-31 | Procter & Gamble | Detergent composition |
EP2377914B1 (en) | 2010-04-19 | 2016-11-09 | The Procter & Gamble Company | Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase |
EP2365058A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition having an excellent anti-encrustation profile |
EP2363456A1 (en) | 2010-03-01 | 2011-09-07 | The Procter & Gamble Company | Solid laundry detergent composition comprising brightener in micronized particulate form |
EP2380957A1 (en) | 2010-04-19 | 2011-10-26 | The Procter & Gamble Company | Solid laundry detergent composition having a dynamic in-wash ph profile |
EP2365054A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising secondary alcohol-based detersive surfactant |
EP2365059A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising C.I. fluorescent brightener 260 in alpha-crystalline form |
US20120067373A1 (en) | 2010-04-15 | 2012-03-22 | Philip Frank Souter | Automatic Dishwashing Detergent Composition |
JP6018044B2 (en) * | 2010-04-15 | 2016-11-02 | ダニスコ・ユーエス・インク | Compositions and methods comprising protease variants |
US20110257069A1 (en) | 2010-04-19 | 2011-10-20 | Stephen Joseph Hodson | Detergent composition |
US8889612B2 (en) | 2010-04-19 | 2014-11-18 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
US20110257062A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Liquid laundry detergent composition comprising a source of peracid and having a ph profile that is controlled with respect to the pka of the source of peracid |
US20110257060A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Laundry detergent composition comprising bleach particles that are suspended within a continuous liquid phase |
ES2565192T3 (en) | 2010-04-23 | 2016-04-01 | The Procter & Gamble Company | Method to perfume |
EP2383329A1 (en) | 2010-04-23 | 2011-11-02 | The Procter & Gamble Company | Particle |
EP2380961B1 (en) | 2010-04-23 | 2018-05-23 | The Procter and Gamble Company | Detergent composition |
ES2579217T3 (en) | 2010-04-23 | 2016-08-08 | The Procter & Gamble Company | Particle |
ES2533368T3 (en) | 2010-04-23 | 2015-04-09 | The Procter & Gamble Company | Dishwasher product |
EP2380478A1 (en) | 2010-04-23 | 2011-10-26 | The Procter & Gamble Company | Automatic dishwashing product |
CN105925556B (en) | 2010-05-06 | 2020-11-13 | 丹尼斯科美国公司 | Compositions and methods comprising subtilisin variants |
EP2395070A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Liquid laundry detergent composition comprising lipase of bacterial origin |
EP2395071A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Solid detergent composition comprising lipase of bacterial origin |
WO2011163457A1 (en) | 2010-06-23 | 2011-12-29 | The Procter & Gamble Company | Product for pre-treatment and laundering of stained fabric |
US20130166040A1 (en) * | 2010-06-24 | 2013-06-27 | The Board Of Trustee Of The University Of Illinois | Reagents and methods for preparing teeth for implantation |
ES2560218T3 (en) | 2010-07-02 | 2016-02-17 | The Procter & Gamble Company | Process for making films from bands of nonwoven material |
BR112013000099A2 (en) | 2010-07-02 | 2016-05-17 | Procter & Gamble | filaments comprising non-woven non-scent active agent fabrics and methods of manufacture thereof |
EP2588589B2 (en) | 2010-07-02 | 2023-07-19 | The Procter & Gamble Company | Process for the production of a detergent product |
BR112013000101A2 (en) | 2010-07-02 | 2016-05-17 | Procter & Gamble | filaments comprising active agent nonwoven webs and methods of manufacture thereof |
CA2803382C (en) | 2010-07-02 | 2015-03-31 | The Procter & Gamble Company | Method for delivering an active agent |
EP2596088B1 (en) | 2010-07-22 | 2016-09-28 | Unilever PLC | Detergent compositions comprising biosurfactant and enzyme |
BR112013000114B1 (en) | 2010-07-22 | 2020-12-29 | Unilever N.V. | cleaning composition and process for cleaning a substrate |
US8685171B2 (en) | 2010-07-29 | 2014-04-01 | The Procter & Gamble Company | Liquid detergent composition |
EP2412792A1 (en) | 2010-07-29 | 2012-02-01 | The Procter & Gamble Company | Liquid detergent composition |
WO2012057781A1 (en) | 2010-10-29 | 2012-05-03 | The Procter & Gamble Company | Cleaning and/or treatment compositions comprising a fungal serine protease |
WO2011026154A2 (en) | 2010-10-29 | 2011-03-03 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
EP2500087B1 (en) | 2011-03-18 | 2018-10-10 | International Flavors & Fragrances Inc. | Microcapsules produced from blended sol-gel precursors |
JP5933688B2 (en) | 2011-05-05 | 2016-06-15 | ザ プロクター アンド ギャンブル カンパニー | Compositions and methods comprising serine protease variants |
CA3111256C (en) | 2011-05-05 | 2023-05-09 | Danisco Us Inc. | Compositions and methods comprising serine protease variants |
US20140371435A9 (en) | 2011-06-03 | 2014-12-18 | Eduardo Torres | Laundry Care Compositions Containing Thiophene Azo Dyes |
EP2537918A1 (en) | 2011-06-20 | 2012-12-26 | The Procter & Gamble Company | Consumer products with lipase comprising coated particles |
US8828920B2 (en) | 2011-06-23 | 2014-09-09 | The Procter & Gamble Company | Product for pre-treatment and laundering of stained fabric |
US20120324655A1 (en) | 2011-06-23 | 2012-12-27 | Nalini Chawla | Product for pre-treatment and laundering of stained fabric |
EP2725912A4 (en) | 2011-06-29 | 2015-03-04 | Solae Llc | Baked food compositions comprising soy whey proteins that have been isolated from processing streams |
EP2540824A1 (en) | 2011-06-30 | 2013-01-02 | The Procter & Gamble Company | Cleaning compositions comprising amylase variants reference to a sequence listing |
EP2551335A1 (en) | 2011-07-25 | 2013-01-30 | The Procter & Gamble Company | Enzyme stabilized liquid detergent composition |
US20130029895A1 (en) | 2011-07-27 | 2013-01-31 | Jean-Luc Phillippe Bettiol | Multiphase liquid detergent composition |
US10246668B2 (en) * | 2011-08-24 | 2019-04-02 | Illinois Tool Works, Inc. | Proportioner-ready bioenzymatic concentrated cleaning product |
PL2584028T3 (en) | 2011-10-19 | 2017-10-31 | Procter & Gamble | Particle |
US20130123162A1 (en) * | 2011-11-10 | 2013-05-16 | The Procter & Gamble Company | Consumer products |
EP3719192A1 (en) | 2012-01-04 | 2020-10-07 | The Procter & Gamble Company | Fibrous structures comprising particles and methods for making same |
DK2623586T3 (en) | 2012-02-03 | 2017-11-13 | Procter & Gamble | COMPOSITIONS AND PROCEDURES FOR LIPASER SURFACE TREATMENT |
MX2014011154A (en) | 2012-03-19 | 2014-12-10 | Procter & Gamble | Laundry care compositions containing dyes. |
WO2013149858A1 (en) | 2012-04-02 | 2013-10-10 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
EP2674475A1 (en) | 2012-06-11 | 2013-12-18 | The Procter & Gamble Company | Detergent composition |
US10246692B2 (en) | 2012-07-12 | 2019-04-02 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
DE102012215107A1 (en) * | 2012-08-24 | 2014-02-27 | Basf Se | Solid dishwashing detergent with improved protease performance |
EP2712915A1 (en) | 2012-10-01 | 2014-04-02 | The Procter and Gamble Company | Methods of treating a surface and compositions for use therein |
MX2015007802A (en) | 2012-12-20 | 2015-09-04 | Procter & Gamble | Detergent composition with silicate coated bleach. |
BR112015021683A2 (en) | 2013-03-05 | 2017-07-18 | Procter & Gamble | mixed sugar compositions |
WO2014143773A1 (en) | 2013-03-15 | 2014-09-18 | Lubrizol Advanced Materials, Inc. | Itaconic acid polymers |
CN105051174B (en) | 2013-03-21 | 2018-04-03 | 诺维信公司 | Polypeptide and their polynucleotides of coding with lipase active |
PL2978830T3 (en) | 2013-03-28 | 2019-08-30 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
JP6188199B2 (en) * | 2013-05-20 | 2017-08-30 | ライオン株式会社 | Dishwasher cleaner |
EP2808372A1 (en) | 2013-05-28 | 2014-12-03 | The Procter and Gamble Company | Surface treatment compositions comprising photochromic dyes |
CN105722495B (en) | 2013-08-15 | 2020-02-28 | 国际香料和香精公司 | Polyurea or polyurethane capsules |
CA2920901A1 (en) | 2013-09-18 | 2015-03-26 | The Procter & Gamble Company | Laundry care compositions containing thiophene azo carboxylate dyes |
WO2015042013A1 (en) | 2013-09-18 | 2015-03-26 | Lubrizol Advanced Materials, Inc. | Stable linear polymers |
CN105555935A (en) | 2013-09-18 | 2016-05-04 | 宝洁公司 | Laundry care composition comprising carboxylate dye |
US9834682B2 (en) | 2013-09-18 | 2017-12-05 | Milliken & Company | Laundry care composition comprising carboxylate dye |
CN105555936A (en) | 2013-09-18 | 2016-05-04 | 宝洁公司 | Laundry care composition comprising carboxylate dye |
EP2857486A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
EP2857487A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
EP2857485A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising alkanolamine-free cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
US9610228B2 (en) | 2013-10-11 | 2017-04-04 | International Flavors & Fragrances Inc. | Terpolymer-coated polymer encapsulated active material |
EP2862597B1 (en) | 2013-10-18 | 2018-01-03 | International Flavors & Fragrances Inc. | Stable, flowable silica capsule formulation |
MX353557B (en) | 2013-11-11 | 2018-01-17 | Int Flavors & Fragrances Inc | Multi-capsule compositions. |
CN105873603A (en) | 2013-12-19 | 2016-08-17 | 雀巢产品技术援助有限公司 | Compositions and methods for reducing cat allergens in the environment |
EP3097174A1 (en) | 2014-01-22 | 2016-11-30 | The Procter & Gamble Company | Method of treating textile fabrics |
WO2015112339A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Fabric treatment composition |
WO2015112338A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Method of treating textile fabrics |
WO2015112341A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Fabric treatment composition |
CA2841024C (en) | 2014-01-30 | 2017-03-07 | The Procter & Gamble Company | Unit dose article |
US10752562B2 (en) | 2014-02-25 | 2020-08-25 | The Procter & Gamble Company | Process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
US9994497B2 (en) | 2014-02-25 | 2018-06-12 | The Procter & Gamble Company | Process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
JP6342508B2 (en) | 2014-02-26 | 2018-06-13 | ザ プロクター アンド ギャンブル カンパニー | Antifoam composition |
EP3116924B1 (en) | 2014-03-14 | 2020-08-19 | Lubrizol Advanced Materials, Inc. | Itaconic acid polymers and copolymers |
WO2015148361A1 (en) | 2014-03-27 | 2015-10-01 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
WO2015148360A1 (en) | 2014-03-27 | 2015-10-01 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
EP2924107A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
EP2924105A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
JP2017518407A (en) | 2014-05-06 | 2017-07-06 | ミリケン・アンド・カンパニーMilliken & Company | Laundry care composition |
EP3152288A1 (en) | 2014-06-06 | 2017-04-12 | The Procter & Gamble Company | Detergent composition comprising polyalkyleneimine polymers |
WO2015191434A2 (en) | 2014-06-09 | 2015-12-17 | Stepan Company | Detergents for cold-water cleaning |
CA2959973A1 (en) | 2014-09-10 | 2016-03-17 | Basf Se | Encapsulated cleaning composition |
US9617502B2 (en) | 2014-09-15 | 2017-04-11 | The Procter & Gamble Company | Detergent compositions containing salts of polyetheramines and polymeric acid |
BR112017005767A2 (en) | 2014-09-25 | 2017-12-12 | Procter & Gamble | cleaning compositions containing a polyetheramine |
US20160090552A1 (en) | 2014-09-25 | 2016-03-31 | The Procter & Gamble Company | Detergent compositions containing a polyetheramine and an anionic soil release polymer |
US9388368B2 (en) | 2014-09-26 | 2016-07-12 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
MX2017006377A (en) | 2014-11-17 | 2017-08-21 | Procter & Gamble | Benefit agent delivery compositions. |
BR112017014650B1 (en) | 2015-01-08 | 2022-10-11 | Stepan Company | USEFUL CLOTHING DETERGENT FOR COLD WATER CLEANING INCLUDING AN ALKYLENE BRIDGE SURFACTANT AND A LIPASE AND A METHOD FOR WASHING A DIRTY TEXTILE ARTICLE IN WATER |
WO2016160407A1 (en) | 2015-03-31 | 2016-10-06 | Stepan Company | Detergents based on alpha-sulfonated fatty ester surfactants |
US9783766B2 (en) | 2015-04-03 | 2017-10-10 | Ecolab Usa Inc. | Enhanced peroxygen stability using anionic surfactant in TAED-containing peroxygen solid |
US10280386B2 (en) | 2015-04-03 | 2019-05-07 | Ecolab Usa Inc. | Enhanced peroxygen stability in multi-dispense TAED-containing peroxygen solid |
WO2016172699A1 (en) | 2015-04-24 | 2016-10-27 | International Flavors & Fragrances Inc. | Delivery systems and methods of preparing the same |
PL3088503T3 (en) | 2015-04-29 | 2018-10-31 | The Procter & Gamble Company | Method of treating a fabric |
US20160319227A1 (en) | 2015-04-29 | 2016-11-03 | The Procter & Gamble Company | Method of treating a fabric |
DK3088505T3 (en) | 2015-04-29 | 2020-08-03 | Procter & Gamble | PROCEDURE FOR TREATMENT OF A TEXTILE FABRIC |
HUE039245T2 (en) | 2015-04-29 | 2018-12-28 | Procter & Gamble | Detergent composition |
EP3088502B1 (en) | 2015-04-29 | 2018-05-23 | The Procter and Gamble Company | Method of treating a fabric |
EP3292173A1 (en) | 2015-05-04 | 2018-03-14 | Milliken & Company | Leuco triphenylmethane colorants as bluing agents in laundry care compositions |
WO2016196555A1 (en) | 2015-06-02 | 2016-12-08 | Stepan Company | Cold-water cleaning method |
CN107835851B (en) | 2015-06-02 | 2020-03-20 | 荷兰联合利华有限公司 | Laundry detergent compositions |
US10226544B2 (en) | 2015-06-05 | 2019-03-12 | International Flavors & Fragrances Inc. | Malodor counteracting compositions |
TR201906232T4 (en) | 2015-06-11 | 2019-05-21 | Unilever Nv | Detergent composition for laundry. |
EP3313966B1 (en) | 2015-06-26 | 2020-07-29 | Unilever PLC | Laundry detergent composition |
WO2016203064A2 (en) * | 2015-10-28 | 2016-12-22 | Novozymes A/S | Detergent composition comprising protease and amylase variants |
US10183087B2 (en) * | 2015-11-10 | 2019-01-22 | American Sterilizer Company | Cleaning and disinfecting composition |
WO2017089093A1 (en) | 2015-11-25 | 2017-06-01 | Unilever N.V. | A liquid detergent composition |
WO2017100051A2 (en) | 2015-12-07 | 2017-06-15 | Stepan Comapny | Cold-water cleaning compositions and methods |
US20170204223A1 (en) | 2016-01-15 | 2017-07-20 | International Flavors & Fragrances Inc. | Polyalkoxy-polyimine adducts for use in delayed release of fragrance ingredients |
EP3205392A1 (en) | 2016-02-12 | 2017-08-16 | Basf Se | Microcapsules and process for preparation of microcapsules |
EP3205393A1 (en) | 2016-02-12 | 2017-08-16 | Basf Se | Process for preparation of microcapsules |
CN108603139B (en) | 2016-02-17 | 2020-12-04 | 荷兰联合利华有限公司 | Whitening composition |
BR112018016674B1 (en) | 2016-02-17 | 2022-06-07 | Unilever Ip Holdings B.V. | Detergent composition for washing clothes and domestic method of treating a fabric |
WO2017143174A1 (en) | 2016-02-18 | 2017-08-24 | International Flavors & Fragrances Inc. | Polyurea capsule compositions |
EP3426757A1 (en) | 2016-03-09 | 2019-01-16 | Basf Se | Encapsulated laundry cleaning composition |
WO2017162378A1 (en) | 2016-03-21 | 2017-09-28 | Unilever Plc | Laundry detergent composition |
US10119100B2 (en) * | 2016-04-07 | 2018-11-06 | The Honest Company, Inc. | Laundry detergent compositions |
CN108779416B (en) | 2016-04-08 | 2021-01-05 | 荷兰联合利华有限公司 | Laundry detergent compositions |
WO2017182295A1 (en) | 2016-04-18 | 2017-10-26 | Basf Se | Liquid cleaning compositions |
CN105820888A (en) * | 2016-04-21 | 2016-08-03 | 刘爱华 | Decontaminating bactericidal clothing detergent |
CN109642188A (en) | 2016-05-20 | 2019-04-16 | 斯特潘公司 | Polyethers amine composition for laundry detergent compositions |
EP3275987A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
EP3275989A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
EP3275985A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
EP3275986B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
EP3275988B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
ES2950434T3 (en) | 2016-09-16 | 2023-10-10 | Int Flavors & Fragrances Inc | Microcapsule compositions stabilized with viscosity control agents |
US20180085291A1 (en) | 2016-09-28 | 2018-03-29 | International Flavors & Fragrances Inc. | Microcapsule compositions containing amino silicone |
BR112019006576A2 (en) | 2016-11-01 | 2019-07-02 | Milliken & Co | leuco dyes as bleaching agents in laundry care compositions |
EP3535362A1 (en) | 2016-11-01 | 2019-09-11 | The Procter and Gamble Company | Leuco colorants as bluing agents in laundry care compositions, packaging, kits and methods thereof |
BR112019008706A2 (en) | 2016-11-01 | 2019-07-16 | Procter & Gamble | leucos dyes as bleaching agents in laundry care compositions |
US20180119056A1 (en) | 2016-11-03 | 2018-05-03 | Milliken & Company | Leuco Triphenylmethane Colorants As Bluing Agents in Laundry Care Compositions |
US10758462B2 (en) | 2016-11-16 | 2020-09-01 | Colgate-Palmolive Company | Oral care composition |
WO2018093356A1 (en) * | 2016-11-16 | 2018-05-24 | Colgate-Palmolive Company | Oral care composition |
US10550443B2 (en) | 2016-12-02 | 2020-02-04 | The Procter & Gamble Company | Cleaning compositions including enzymes |
CA3044420C (en) | 2016-12-02 | 2022-03-22 | The Procter & Gamble Company | Cleaning compositions including enzymes |
MX2019006421A (en) | 2016-12-02 | 2019-08-01 | Procter & Gamble | Cleaning compositions including enzymes. |
BR112019011999B1 (en) | 2016-12-15 | 2022-11-08 | Unilever Ip Holdings B.V | COMPOSITION OF AQUEOUS LIQUID DETERGENT FOR WASHING CLOTHES AND DOMESTIC METHOD OF TREATMENT OF A FABRIC |
EP3339423A1 (en) | 2016-12-22 | 2018-06-27 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
US11697904B2 (en) | 2017-01-27 | 2023-07-11 | The Procter & Gamble Company | Active agent-containing articles that exhibit consumer acceptable article in-use properties |
WO2018140472A1 (en) | 2017-01-27 | 2018-08-02 | The Procter & Gamble Company | Active agent-containing articles that exhibit consumer acceptable article in-use properties |
US11697906B2 (en) | 2017-01-27 | 2023-07-11 | The Procter & Gamble Company | Active agent-containing articles and product-shipping assemblies for containing the same |
US11697905B2 (en) | 2017-01-27 | 2023-07-11 | The Procter & Gamble Company | Active agent-containing articles that exhibit consumer acceptable article in-use properties |
RU2019123965A (en) | 2017-02-01 | 2021-02-01 | Дзе Проктер Энд Гэмбл Компани | CLEANING COMPOSITIONS CONTAINING AMYLASE VERSIONS |
US20180346648A1 (en) | 2017-05-30 | 2018-12-06 | International Flavors & Fragrances Inc. | Branched polyethyleneimine microcapsules |
BR112020000205B1 (en) | 2017-07-07 | 2023-10-31 | Unilever Ip Holdings B.V. | CLEANING COMPOSITION FOR WASHING FABRICS AND HOUSEHOLD METHOD OF TREATING A FABRIC |
CN110869480B (en) | 2017-07-07 | 2021-08-13 | 联合利华知识产权控股有限公司 | Whitening composition |
TWI715878B (en) | 2017-10-12 | 2021-01-11 | 美商美力肯及公司 | Leuco colorants and compositions |
CA3075090A1 (en) | 2017-10-12 | 2019-04-18 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions |
CA3074613A1 (en) | 2017-10-12 | 2019-04-18 | The Procter & Gamble Company | Leuco colorants in combination with a second whitening agent as bluing agents in laundry care compositions |
MX2020003103A (en) | 2017-10-12 | 2020-07-28 | Procter & Gamble | Leuco colorants as bluing agents in laundry care composition. |
BR112020008476B1 (en) | 2017-11-01 | 2023-11-21 | Milliken & Company | LEUCO COMPOUND |
WO2019105675A1 (en) | 2017-11-30 | 2019-06-06 | Unilever Plc | Detergent composition comprising protease |
EP3502227A1 (en) | 2017-12-19 | 2019-06-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
EP3502244A1 (en) | 2017-12-19 | 2019-06-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
EP3502246A1 (en) | 2017-12-19 | 2019-06-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
EP3502245A1 (en) | 2017-12-19 | 2019-06-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
CA3087284C (en) | 2018-01-26 | 2023-05-02 | The Procter & Gamble Company | Water-soluble unit dose articles comprising enzyme |
EP3533859A1 (en) | 2018-02-28 | 2019-09-04 | The Procter & Gamble Company | Cleaning compositions |
US20190264140A1 (en) | 2018-02-28 | 2019-08-29 | The Procter & Gamble Company | Methods of cleaning |
CN112119147B (en) | 2018-05-17 | 2023-09-29 | 联合利华知识产权控股有限公司 | cleaning composition |
EP3810743B1 (en) | 2018-06-15 | 2024-03-13 | Ecolab USA Inc. | Enhanced peroxygen stability using fatty acid in bleach activating agent containing peroxygen solid |
WO2019245838A1 (en) | 2018-06-19 | 2019-12-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
EP3810769A1 (en) | 2018-06-19 | 2021-04-28 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
CN110713866B (en) * | 2018-07-13 | 2022-05-03 | 花王株式会社 | Liquid detergent composition for clothing |
US20200032178A1 (en) | 2018-07-27 | 2020-01-30 | The Procter & Gamble Company | Water-soluble unit dose articles comprising water-soluble fibrous structures and particles |
CN112703246A (en) | 2018-09-17 | 2021-04-23 | 联合利华知识产权控股有限公司 | Detergent composition |
BR112021009828A2 (en) | 2018-11-20 | 2021-08-17 | Unilever Ip Holdings B.V. | liquid detergent composition, method of treating a fabric substrate and use of a sterol esterase enzyme |
CN113056549B (en) | 2018-11-20 | 2023-03-10 | 联合利华知识产权控股有限公司 | Detergent composition |
WO2020104156A1 (en) | 2018-11-20 | 2020-05-28 | Unilever Plc | Detergent composition |
BR112021009789A2 (en) | 2018-11-20 | 2021-08-17 | Unilever Ip Holdings B.V. | detergent composition, method of treating a fabric substrate and use of an esterase enzyme |
WO2020104159A1 (en) | 2018-11-20 | 2020-05-28 | Unilever Plc | Detergent composition |
JP7364677B2 (en) | 2018-12-14 | 2023-10-18 | ザ プロクター アンド ギャンブル カンパニー | Foamable fiber structure containing particles and method for producing the same |
EP3897955B1 (en) | 2018-12-18 | 2024-02-07 | International Flavors & Fragrances Inc. | Hydroxyethyl cellulose microcapsules |
US10988715B2 (en) | 2019-03-14 | 2021-04-27 | The Procter & Gamble Company | Method for treating cotton |
MX2021011104A (en) | 2019-03-14 | 2021-10-22 | Procter & Gamble | Cleaning compositions comprising enzymes. |
JP7275297B2 (en) | 2019-03-14 | 2023-05-17 | ザ プロクター アンド ギャンブル カンパニー | Cleaning composition containing enzymes |
EP3969556A1 (en) | 2019-05-16 | 2022-03-23 | Unilever Global Ip Limited | Laundry composition |
EP3741283A1 (en) | 2019-05-22 | 2020-11-25 | The Procter & Gamble Company | Automatic dishwashing method |
EP3976775A1 (en) | 2019-05-24 | 2022-04-06 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
CN114040972A (en) | 2019-06-24 | 2022-02-11 | 宝洁公司 | Cleaning compositions comprising amylase variants |
US20220364022A1 (en) | 2019-06-28 | 2022-11-17 | Conopco, Inc., D/B/A Unilever | Detergent composition |
CN114008183A (en) | 2019-06-28 | 2022-02-01 | 联合利华知识产权控股有限公司 | Detergent composition |
US20220372397A1 (en) | 2019-06-28 | 2022-11-24 | Conopco, Inc., D/B/A Unilever | Detergent composition |
EP3990602A1 (en) | 2019-06-28 | 2022-05-04 | Unilever Global IP Limited | Detergent composition |
US20220372408A1 (en) | 2019-06-28 | 2022-11-24 | Conopco, Inc., D/B/A Unilever | Detergent composition |
WO2020260006A1 (en) | 2019-06-28 | 2020-12-30 | Unilever Plc | Detergent compositions |
WO2021043764A1 (en) | 2019-09-02 | 2021-03-11 | Unilever Global Ip Limited | Detergent composition |
BR112022004470A2 (en) | 2019-09-19 | 2022-05-31 | Unilever Ip Holdings B V | Detergent composition and method for non-oxidative washing of fabric stains |
AR120142A1 (en) | 2019-10-07 | 2022-02-02 | Unilever Nv | DETERGENT COMPOSITION |
US20210122998A1 (en) | 2019-10-24 | 2021-04-29 | The Procter & Gamble Company | Automatic dishwashing detergent composition comprising an amylase |
US20210148044A1 (en) | 2019-11-15 | 2021-05-20 | The Procter & Gamble Company | Graphic-Containing Soluble Articles and Methods for Making Same |
EP3835396A1 (en) | 2019-12-09 | 2021-06-16 | The Procter & Gamble Company | A detergent composition comprising a polymer |
BR112022015120A2 (en) | 2020-01-29 | 2022-12-13 | Unilever Ip Holdings B V | TRANSPARENT PLASTIC CONTAINER AND TRANSPARENT PLASTIC CONTAINER MANUFACTURING PROCESS |
EP3862412A1 (en) | 2020-02-04 | 2021-08-11 | The Procter & Gamble Company | Detergent composition |
EP4162016A1 (en) | 2020-06-05 | 2023-04-12 | The Procter & Gamble Company | Detergent compositions containing a branched surfactant |
EP4162018B1 (en) | 2020-06-08 | 2024-01-31 | Unilever IP Holdings B.V. | Method of improving protease activity |
CA3186847A1 (en) | 2020-08-04 | 2022-02-10 | Nevena Lukic | Automatic dishwashing method |
CA3187735A1 (en) | 2020-08-04 | 2022-02-10 | Nina Elizabeth GRAY | Automatic dishwashing method and pack |
WO2022031309A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
WO2022031311A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
EP4204530A1 (en) | 2020-08-28 | 2023-07-05 | Unilever IP Holdings B.V. | Detergent composition |
BR112023003008A2 (en) | 2020-08-28 | 2023-04-04 | Unilever Ip Holdings B V | SECONDARY ALKAN SULFONATE SURFACTANT (SAS), DETERGENT COMPOSITION AND TREATMENT METHOD OF A TEXTILE ARTICLE |
US20230303951A1 (en) | 2020-08-28 | 2023-09-28 | Conopco, Inc., D/B/A Unilever | Detergent composition |
CN116096703A (en) | 2020-08-28 | 2023-05-09 | 联合利华知识产权控股有限公司 | Surfactant and detergent composition |
WO2022043042A1 (en) | 2020-08-28 | 2022-03-03 | Unilever Ip Holdings B.V. | Detergent composition |
WO2022093189A1 (en) | 2020-10-27 | 2022-05-05 | Milliken & Company | Compositions comprising leuco compounds and colorants |
CA3196371A1 (en) | 2020-10-29 | 2022-05-05 | Catherine Jones | Cleaning composition comprising alginate lyase enzymes |
WO2022108766A1 (en) | 2020-11-17 | 2022-05-27 | The Procter & Gamble Company | Automatic dishwashing composition comprising amphiphilic graft polymer |
CA3197110A1 (en) | 2020-11-17 | 2022-05-27 | Patrick Firmin August Delplancke | Automatic dishwashing method with alkaline rinse |
EP4001388A1 (en) | 2020-11-17 | 2022-05-25 | The Procter & Gamble Company | Automatic dishwashing method with amphiphilic graft polymer in the rinse |
EP4006131A1 (en) | 2020-11-30 | 2022-06-01 | The Procter & Gamble Company | Method of laundering fabric |
US20240010951A1 (en) | 2020-12-07 | 2024-01-11 | Conopco Inc., D/B/A Unilever | Detergent compositions |
WO2022122481A1 (en) | 2020-12-07 | 2022-06-16 | Unilever Ip Holdings B.V. | Detergent compositions |
JP2023551014A (en) | 2020-12-23 | 2023-12-06 | ビーエーエスエフ ソシエタス・ヨーロピア | Amphiphilic alkoxylated polyamines and their uses |
MX2023005793A (en) | 2021-03-15 | 2023-05-29 | Procter & Gamble | Cleaning compositions containing polypeptide variants. |
WO2022235720A1 (en) | 2021-05-05 | 2022-11-10 | The Procter & Gamble Company | Methods for making cleaning compositions and detecting soils |
EP4086330A1 (en) | 2021-05-06 | 2022-11-09 | The Procter & Gamble Company | Surface treatment |
EP4108767A1 (en) | 2021-06-22 | 2022-12-28 | The Procter & Gamble Company | Cleaning or treatment compositions containing nuclease enzymes |
EP4123007A1 (en) | 2021-07-19 | 2023-01-25 | The Procter & Gamble Company | Fabric treatment using bacterial spores |
EP4123006A1 (en) | 2021-07-19 | 2023-01-25 | The Procter & Gamble Company | Composition comprising spores and pro-perfume materials |
EP4124383A1 (en) | 2021-07-27 | 2023-02-01 | International Flavors & Fragrances Inc. | Biodegradable microcapsules |
CA3228918A1 (en) | 2021-08-10 | 2023-02-16 | Nippon Shokubai Co., Ltd. | Polyalkylene-oxide-containing compound |
CN117957300A (en) | 2021-09-20 | 2024-04-30 | 联合利华知识产权控股有限公司 | Detergent composition |
EP4154974A1 (en) | 2021-09-23 | 2023-03-29 | International Flavors & Fragrances Inc. | Biodegradable microcapsules |
WO2023064749A1 (en) | 2021-10-14 | 2023-04-20 | The Procter & Gamble Company | A fabric and home care product comprising cationic soil release polymer and lipase enzyme |
EP4194537A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
EP4194536A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
WO2023114795A1 (en) | 2021-12-16 | 2023-06-22 | The Procter & Gamble Company | Automatic dishwashing composition comprising a protease |
WO2023114793A1 (en) | 2021-12-16 | 2023-06-22 | The Procter & Gamble Company | Home care composition |
US20230265358A1 (en) | 2021-12-16 | 2023-08-24 | The Procter & Gamble Company | Home care composition comprising an amylase |
US20230365897A1 (en) | 2021-12-16 | 2023-11-16 | The Procter & Gamble Company | Fabric and home care composition including a protease |
EP4212239A1 (en) | 2022-01-14 | 2023-07-19 | International Flavors & Fragrances Inc. | Biodegradable prepolymer microcapsules |
EP4273209A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Machine-cleaning compositions containing enzymes |
EP4273210A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Detergent compositions containing enzymes |
EP4279571A1 (en) | 2022-05-19 | 2023-11-22 | The Procter & Gamble Company | Laundry composition comprising spores |
WO2023227356A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition containing enzyme |
WO2023227331A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition comprising a specific methyl ester ethoxylate surfactant and a lipase |
WO2023227375A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an aminocarboxylate, an organic acid and a fragrance |
WO2023227332A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer and a protease |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988008033A1 (en) * | 1987-04-10 | 1988-10-20 | Amgen Inc. | Subtilisin analogs |
WO1989006279A1 (en) * | 1988-01-07 | 1989-07-13 | Novo-Nordisk A/S | Mutated subtilisin genes |
WO1989009830A1 (en) * | 1988-04-12 | 1989-10-19 | Genex Corporation | Subtilisin mutations |
EP0398539A1 (en) * | 1989-05-17 | 1990-11-22 | Amgen Inc. | Multiply mutated subtilisins |
EP0405901A1 (en) * | 1989-06-26 | 1991-01-02 | Unilever Plc | Enzymatic detergent compositions |
WO1991006637A1 (en) * | 1989-10-31 | 1991-05-16 | Genencor International, Inc. | Subtilisin mutants |
EP0516200A1 (en) * | 1991-05-01 | 1992-12-02 | Unilever N.V. | Detergent compositions containing stabilized enzymes |
Family Cites Families (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US34606A (en) * | 1862-03-04 | Improvement in machines for combing cotton | ||
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5204015A (en) * | 1984-05-29 | 1993-04-20 | Genencor International, Inc. | Subtilisin mutants |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
US5182204A (en) * | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
US4634551A (en) * | 1985-06-03 | 1987-01-06 | Procter & Gamble Company | Bleaching compounds and compositions comprising fatty peroxyacids salts thereof and precursors therefor having amide moieties in the fatty chain |
US4990452A (en) * | 1986-02-12 | 1991-02-05 | Genex Corporation | Combining mutations for stabilization of subtilisin |
IE65767B1 (en) * | 1986-04-30 | 1995-11-15 | Genencor Int | Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors |
US4686063A (en) * | 1986-09-12 | 1987-08-11 | The Procter & Gamble Company | Fatty peroxyacids or salts thereof having amide moieties in the fatty chain and low levels of exotherm control agents |
US5260207A (en) * | 1987-04-06 | 1993-11-09 | Enzon Labs Inc. | Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
EP0353250B1 (en) * | 1987-04-06 | 1999-09-08 | Novo Nordisk A/S | The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
DK571587D0 (en) * | 1987-11-02 | 1987-11-02 | Novo Industri As | ENZYMATIC DETERGENT COMPOSITION |
US4844821A (en) * | 1988-02-10 | 1989-07-04 | The Procter & Gamble Company | Stable liquid laundry detergent/fabric conditioning composition |
GB8803114D0 (en) * | 1988-02-11 | 1988-03-09 | Bp Chem Int Ltd | Bleach activators in detergent compositions |
PT89702B (en) * | 1988-02-11 | 1994-04-29 | Gist Brocades Nv | PROCESS FOR PREPARING NEW PROTEOLITIC ENZYMES AND DETERGENTS THAT CONTAINS THEM |
US5324653A (en) * | 1988-02-11 | 1994-06-28 | Gist-Brocades N.V. | Recombinant genetic means for the production of serine protease muteins |
US5116741A (en) * | 1988-04-12 | 1992-05-26 | Genex Corporation | Biosynthetic uses of thermostable proteases |
GB8810954D0 (en) * | 1988-05-09 | 1988-06-15 | Unilever Plc | Enzymatic detergent & bleaching composition |
US5118623A (en) * | 1988-05-27 | 1992-06-02 | Solvay Enzymes, Inc. | Bleach stable enzymes |
DK316989D0 (en) * | 1989-06-26 | 1989-06-26 | Novo Nordisk As | ENZYMES |
WO1991000335A1 (en) * | 1989-06-26 | 1991-01-10 | Unilever Plc | Enzymatic detergent compositions |
JPH0372876A (en) * | 1989-08-11 | 1991-03-28 | Mitsubishi Gas Chem Co Inc | New alkaline protease |
DK271490D0 (en) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | detergent composition |
EP0563169B2 (en) * | 1990-12-21 | 2006-04-12 | Novozymes A/S | ENZYME MUTANTS HAVING A LOW DEGREE OF VARIATION OF THE MOLECULAR CHARGE OVER A pH RANGE |
DK0583339T3 (en) * | 1991-05-01 | 1999-04-19 | Novo Nordisk As | Stabilized enzymes and detergent compositions |
US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
PT717778E (en) * | 1992-07-17 | 2008-01-16 | Genencor Int | High alkaline serine proteases. |
JPH08502546A (en) * | 1992-10-23 | 1996-03-19 | ザ、プロクター、エンド、ギャンブル、カンパニー | Granular detergent with protease enzyme and bleach |
DK39093D0 (en) * | 1993-04-01 | 1993-04-01 | Novo Nordisk As | ENZYME |
US5505938A (en) * | 1993-09-30 | 1996-04-09 | Lever Brothers Company, Division Of Conopco, Inc. | Straight chain saturated or unsaturated C8 -C18 alkyl aldonolactone esters and an enzymatic process for their preparation |
MA23346A1 (en) * | 1993-10-14 | 1995-04-01 | Genencor Int | VARIANTS OF THE SUB-USE |
CZ105396A3 (en) * | 1993-10-14 | 1996-09-11 | Procter & Gamble | Cleaning agent, agent for cleaning fabrics, agent for washing dishes, washing agent, method of cleaning fabrics, method of washing dishes and washing process |
CZ105296A3 (en) * | 1993-10-14 | 1996-09-11 | Procter & Gamble | Bleaching agent containing protease enzymes, cleansing agents, agent for cleaning fabrics and fabric cleaning method |
AU8015094A (en) * | 1993-11-03 | 1995-05-23 | Procter & Gamble Company, The | Control of calcium carbonate precipitation in automatic dishwashing |
CA2175329C (en) * | 1993-11-03 | 2000-01-18 | Eugene Steven Sadlowski | Control of calcium carbonate precipitation in automatic dishwashing |
US5460747A (en) * | 1994-08-31 | 1995-10-24 | The Procter & Gamble Co. | Multiple-substituted bleach activators |
US5578136A (en) * | 1994-08-31 | 1996-11-26 | The Procter & Gamble Company | Automatic dishwashing compositions comprising quaternary substituted bleach activators |
US5686015A (en) * | 1994-08-31 | 1997-11-11 | The Procter & Gamble Company | Quaternary substituted bleach activators |
CN1172498A (en) * | 1994-11-18 | 1998-02-04 | 普罗格特-甘布尔公司 | Detergent compositions containing lipase and protease |
CN1136311C (en) * | 1996-11-04 | 2004-01-28 | 诺沃奇梅兹有限公司 | Subtilase variants and compositions |
EP2278001B1 (en) * | 1996-11-04 | 2013-10-23 | Novozymes A/S | Protease variants and compositions |
-
1994
- 1994-10-13 CZ CZ961053A patent/CZ105396A3/en unknown
- 1994-10-13 AU AU80797/94A patent/AU8079794A/en not_active Abandoned
- 1994-10-13 ES ES94931872T patent/ES2287931T3/en not_active Expired - Lifetime
- 1994-10-13 BR BR9407834A patent/BR9407834A/en not_active IP Right Cessation
- 1994-10-13 CA CA002173105A patent/CA2173105C/en not_active Expired - Lifetime
- 1994-10-13 HU HU9600960A patent/HU219851B/en unknown
- 1994-10-13 US US08/322,676 patent/US5679630A/en not_active Expired - Lifetime
- 1994-10-13 EP EP94931872A patent/EP0723579B1/en not_active Expired - Lifetime
- 1994-10-13 CN CN94194485A patent/CN1082999C/en not_active Expired - Lifetime
- 1994-10-13 WO PCT/US1994/011776 patent/WO1995010591A1/en active IP Right Grant
- 1994-10-13 DE DE69434962T patent/DE69434962T2/en not_active Expired - Lifetime
- 1994-10-13 AT AT94931872T patent/ATE361355T1/en not_active IP Right Cessation
- 1994-10-13 JP JP7512131A patent/JP2888985B2/en not_active Expired - Lifetime
-
1995
- 1995-01-18 TW TW084100416A patent/TW306931B/zh active
-
1997
- 1997-07-22 US US08/898,218 patent/US6017871A/en not_active Expired - Lifetime
-
1998
- 1998-10-27 JP JP30591498A patent/JP3776244B2/en not_active Expired - Lifetime
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988008033A1 (en) * | 1987-04-10 | 1988-10-20 | Amgen Inc. | Subtilisin analogs |
WO1989006279A1 (en) * | 1988-01-07 | 1989-07-13 | Novo-Nordisk A/S | Mutated subtilisin genes |
WO1989009830A1 (en) * | 1988-04-12 | 1989-10-19 | Genex Corporation | Subtilisin mutations |
EP0398539A1 (en) * | 1989-05-17 | 1990-11-22 | Amgen Inc. | Multiply mutated subtilisins |
EP0405901A1 (en) * | 1989-06-26 | 1991-01-02 | Unilever Plc | Enzymatic detergent compositions |
WO1991006637A1 (en) * | 1989-10-31 | 1991-05-16 | Genencor International, Inc. | Subtilisin mutants |
EP0516200A1 (en) * | 1991-05-01 | 1992-12-02 | Unilever N.V. | Detergent compositions containing stabilized enzymes |
Cited By (131)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6017871A (en) * | 1993-10-14 | 2000-01-25 | The Procter & Gamble Company | Protease-containing cleaning compositions |
US5677272A (en) * | 1993-10-14 | 1997-10-14 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
WO1996016154A1 (en) * | 1994-11-18 | 1996-05-30 | The Procter & Gamble Company | Detergent compositions containing lipase and protease |
US5707950A (en) * | 1994-11-18 | 1998-01-13 | The Procter & Gamble Company | Detergent compositions containing lipase and protease |
US6509021B1 (en) | 1995-08-23 | 2003-01-21 | Henkel Kommanditgesellschaft Auf Aktien | Use of mutated subtilisin protease in cosmetic products |
WO1997007770A1 (en) * | 1995-08-23 | 1997-03-06 | Henkel Kommanditgesellschaft Auf Aktien | Use of mutated subtilisin protease in cosmetic products |
WO1997042282A1 (en) | 1996-05-03 | 1997-11-13 | The Procter & Gamble Company | Detergent compositions comprising polyamine polymers with improved soil dispersancy |
JPH11508318A (en) * | 1996-05-03 | 1999-07-21 | ザ、プロクター、エンド、ギャンブル、カンパニー | Liquid laundry detergent composition containing cotton soil release polymer and protease enzyme |
EP0839905A1 (en) * | 1996-11-05 | 1998-05-06 | The Procter & Gamble Company | Foam detergent composition with enzymes |
US6156722A (en) * | 1996-12-31 | 2000-12-05 | The Procter & Gamble Company | Laundry detergent compositions comprising dye fixatives |
US6140292A (en) * | 1996-12-31 | 2000-10-31 | The Procter & Gamble Company | Laundry detergent compositions with polyamide-polyamines |
US6194370B1 (en) | 1996-12-31 | 2001-02-27 | The Procter & Gamble Company | Cost effective stain and soil removal aqueous heavy duty liquid laundry detergent compositions |
US6221825B1 (en) | 1996-12-31 | 2001-04-24 | The Procter & Gamble Company | Thickened, highly aqueous liquid detergent compositions |
US6369011B1 (en) * | 1997-06-04 | 2002-04-09 | The Procter & Gamble Company | Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same |
WO1998055634A1 (en) * | 1997-06-04 | 1998-12-10 | The Procter & Gamble Company | Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same |
WO1998055579A1 (en) * | 1997-06-04 | 1998-12-10 | The Procter & Gamble Company | Mixed protease enzyme systems for cleaning protein based soils and compositions incorporating same |
WO1999020726A1 (en) * | 1997-10-23 | 1999-04-29 | The Procter & Gamble Company | Bleaching compositions comprising multiply-substituted protease variants |
WO1999020723A3 (en) * | 1997-10-23 | 2001-01-04 | Procter & Gamble | Multiply-substituted protease variant and amylase variant-containing cleaning compositions |
EP2284272A1 (en) | 1998-06-10 | 2011-02-16 | Novozymes A/S | Mannanases |
EP2287318A1 (en) | 1998-06-10 | 2011-02-23 | Novozymes A/S | Mannanases |
US6831053B1 (en) | 1998-10-23 | 2004-12-14 | The Procter & Gamble Company | Bleaching compositions comprising multiply-substituted protease variants |
US6764986B1 (en) | 1999-07-08 | 2004-07-20 | Procter & Gamble Company | Process for producing particles of amine reaction products |
US6740713B1 (en) | 1999-07-08 | 2004-05-25 | Procter & Gamble Company | Process for producing particles of amine reaction products |
US6972276B1 (en) | 1999-07-09 | 2005-12-06 | Procter & Gamble Company | Process for making amine compounds |
EP1754774A2 (en) | 1999-08-10 | 2007-02-21 | The Procter and Gamble Company | Detergent compositions comprising hydrotropes |
WO2001068821A3 (en) * | 2000-03-14 | 2002-04-04 | Novozymes As | Novel subtilase enzymes having an improved wash performance on egg stains |
AU2001239214B8 (en) * | 2000-03-14 | 2006-07-20 | Maxygen, Inc. | Novel subtilase enzymes having an improved wash performance on egg stains |
US6777218B1 (en) | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
AU2001239214B2 (en) * | 2000-03-14 | 2006-06-08 | Maxygen, Inc. | Novel subtilase enzymes having an improved wash performance on egg stains |
WO2001075087A2 (en) | 2000-04-03 | 2001-10-11 | Maxygen, Inc. | Subtilisin variants |
WO2001075087A3 (en) * | 2000-04-03 | 2002-12-19 | Maxygen Inc | Subtilisin variants |
US6902922B2 (en) | 2000-04-03 | 2005-06-07 | Novozymes A/S | Subtilisin variants |
US7220566B2 (en) | 2000-04-03 | 2007-05-22 | Novozymes A/S | Subtilisin variants |
EP1241112A2 (en) | 2001-03-15 | 2002-09-18 | The Procter & Gamble Company | Flexible multiple compartment pouch |
US7153820B2 (en) | 2001-08-13 | 2006-12-26 | Ecolab Inc. | Solid detergent composition and method for solidifying a detergent composition |
WO2003041667A2 (en) * | 2001-11-13 | 2003-05-22 | The Procter & Gamble Company | Compositions containing enzymes stabilized with certain osmo-protectants and methods for using such compositions in personal care |
WO2003041667A3 (en) * | 2001-11-13 | 2003-07-31 | Procter & Gamble | Compositions containing enzymes stabilized with certain osmo-protectants and methods for using such compositions in personal care |
WO2004045576A1 (en) * | 2002-11-18 | 2004-06-03 | Unilever Plc | Viscoelastic cleansing gel with surfactant solutions containing polysaccharides and their derivative polysaccharide hydrocolloids |
US7811076B2 (en) | 2003-12-23 | 2010-10-12 | Henkel Ag & Co. Kgaa | Alkaline protease and washing and cleaning products containing said novel alkaline protease |
US7691618B2 (en) | 2004-04-23 | 2010-04-06 | Henkel Ag & Co. Kgaa | Alkaline proteases and detergents and cleaners comprising these alkaline proteases |
WO2005116180A1 (en) | 2004-05-17 | 2005-12-08 | The Procter & Gamble Company | Bleaching composition comprising a carbohydrate oxidase |
WO2005124012A1 (en) * | 2004-06-18 | 2005-12-29 | Henkel Kommanditgesellschaft Auf Aktien | New enzymatic bleaching system |
US7686892B2 (en) | 2004-11-19 | 2010-03-30 | The Procter & Gamble Company | Whiteness perception compositions |
US7846268B2 (en) | 2004-11-19 | 2010-12-07 | The Procter & Gamble Company | Whiteness perception compositions comprising a dye-polymer conjugate |
EP1700907A1 (en) | 2005-03-11 | 2006-09-13 | Unilever N.V. | Liquid bleaching composition |
EP1700904A1 (en) | 2005-03-11 | 2006-09-13 | Unilever N.V. | Liquid detergent composition |
US9109068B2 (en) | 2005-07-21 | 2015-08-18 | Akzo Nobel N.V. | Hybrid copolymer compositions |
US9321873B2 (en) | 2005-07-21 | 2016-04-26 | Akzo Nobel N.V. | Hybrid copolymer compositions for personal care applications |
US7901772B2 (en) | 2005-09-27 | 2011-03-08 | The Procter & Gamble Company | Microcapsule and method of producing same |
US8460792B2 (en) | 2005-09-27 | 2013-06-11 | The Procter & Gamble Company | Microcapsule and method of producing same |
US8785172B2 (en) | 2006-04-20 | 2014-07-22 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
WO2007122175A1 (en) * | 2006-04-20 | 2007-11-01 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
US9200239B2 (en) | 2006-04-20 | 2015-12-01 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
EP2301517A1 (en) | 2006-08-01 | 2011-03-30 | The Procter & Gamble Company | Benefit agent containing delivery particle |
WO2008028896A2 (en) | 2006-09-08 | 2008-03-13 | Henkel Ag & Co. Kgaa | High-concentration enzyme granules and detergents or cleaners comprising such high-concentration enzyme granules |
US11261406B2 (en) | 2007-02-15 | 2022-03-01 | Ecolab Usa Inc. | Fast dissolving solid detergent |
US10577565B2 (en) | 2007-02-15 | 2020-03-03 | Ecolab Usa Inc. | Fast dissolving solid detergent |
US10005986B2 (en) | 2007-02-15 | 2018-06-26 | Ecolab Usa Inc. | Fast dissolving solid detergent |
EP3339412A1 (en) | 2007-02-15 | 2018-06-27 | Ecolab Usa Inc. | Fast dissolving solid detergent |
EP2617804A1 (en) | 2007-02-15 | 2013-07-24 | Ecolab Inc. | Fast dissolving solid detergent |
US9267097B2 (en) | 2007-02-15 | 2016-02-23 | Ecolab Usa Inc. | Fast dissolving solid detergent |
EP2677023A2 (en) | 2007-10-18 | 2013-12-25 | Ecolab Inc. | Pressed, waxy, solid cleaning compositions and methods of making them |
EP3438235A1 (en) | 2007-10-18 | 2019-02-06 | Ecolab USA Inc. | Pressed, waxy, solid cleaning compositions and methods of making them |
US10844327B2 (en) | 2008-03-14 | 2020-11-24 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
US8680034B2 (en) | 2008-03-14 | 2014-03-25 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
US8008241B2 (en) * | 2008-03-14 | 2011-08-30 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
US8980814B2 (en) | 2008-03-14 | 2015-03-17 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
JP2011513574A (en) * | 2008-03-14 | 2011-04-28 | ザ プロクター アンド ギャンブル カンパニー | Automatic dishwashing detergent composition |
US8978088B2 (en) | 2008-06-13 | 2015-03-10 | Rovi Guides, Inc. | Systems and methods for displaying media content and media guidance information |
WO2010108002A1 (en) | 2009-03-18 | 2010-09-23 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives |
WO2010108000A1 (en) | 2009-03-18 | 2010-09-23 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes |
US8088722B2 (en) | 2009-05-26 | 2012-01-03 | The Procter & Gamble Company | Aqueous liquid composition for pre-treating soiled dishware |
WO2011005830A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Laundry detergent composition comprising low level of sulphate |
WO2011005623A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Laundry detergent composition comprising low level of bleach |
JP2014507489A (en) * | 2010-12-17 | 2014-03-27 | ビーエーエスエフ ソシエタス・ヨーロピア | Storage-stable liquid detergent and detergent containing protease and cellulase |
EP2792737A1 (en) | 2011-05-20 | 2014-10-22 | Ecolab USA Inc. | Non-phosphate detergents and non-phosphoric acids in an alternating alkali/acid system for warewashing |
WO2012160498A2 (en) | 2011-05-20 | 2012-11-29 | Ecolab Usa Inc. | Acid formulations for use in a system for warewashing |
EP2902471A1 (en) | 2011-05-20 | 2015-08-05 | Ecolab USA Inc. | Non-phosphate detergents and non-phosphoric acids in an alternating alkali/acid system for warewashing |
US10167458B2 (en) | 2011-06-30 | 2019-01-01 | Novozymes A/S | Alpha-amylase variants |
US9434932B2 (en) | 2011-06-30 | 2016-09-06 | Novozymes A/S | Alpha-amylase variants |
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US8636918B2 (en) | 2011-08-05 | 2014-01-28 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide hybrid polymer composition and methods of controlling hard water scale |
US8841246B2 (en) | 2011-08-05 | 2014-09-23 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide hybrid polymer composition and methods of improving drainage |
US8679366B2 (en) | 2011-08-05 | 2014-03-25 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide graft polymer composition and methods of controlling hard water scale |
US9309490B2 (en) | 2011-08-05 | 2016-04-12 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide graft polymer compositon and methods of improving drainage |
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US8853144B2 (en) | 2011-08-05 | 2014-10-07 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide graft polymer composition and methods of improving drainage |
US9051406B2 (en) | 2011-11-04 | 2015-06-09 | Akzo Nobel Chemicals International B.V. | Graft dendrite copolymers, and methods for producing the same |
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US8945314B2 (en) | 2012-07-30 | 2015-02-03 | Ecolab Usa Inc. | Biodegradable stability binding agent for a solid detergent |
EP3470516A1 (en) | 2012-09-10 | 2019-04-17 | Ecolab USA Inc. | Stable liquid manual dishwashing compositions containing enzymes |
EP3456809A1 (en) | 2012-10-04 | 2019-03-20 | Ecolab USA, Inc. | Pre-soak technology for laundry and other hard surface cleaning |
EP3279304A1 (en) | 2013-09-09 | 2018-02-07 | Ecolab USA Inc. | Synergistic stain removal through novel chelator combination |
WO2015032447A1 (en) | 2013-09-09 | 2015-03-12 | Ecolab Usa Inc. | Synergistic stain removal through novel chelator combination |
WO2015032451A1 (en) | 2013-09-09 | 2015-03-12 | Ecolab Usa Inc. | Synergistic stain removal through novel chelator combination |
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US11103118B2 (en) | 2014-08-01 | 2021-08-31 | Ecolab Usa Inc. | Method of manual surface cleaning using cleaning textiles and of washing said cleaning textiles |
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CN117701487B (en) * | 2024-02-05 | 2024-05-03 | 内蒙古昆明卷烟有限责任公司 | Bacillus licheniformis engineering bacteria for efficiently expressing amylase genes and application thereof |
Also Published As
Publication number | Publication date |
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US6017871A (en) | 2000-01-25 |
JP2888985B2 (en) | 1999-05-10 |
BR9407834A (en) | 1997-05-13 |
CZ105396A3 (en) | 1996-09-11 |
US5679630A (en) | 1997-10-21 |
JP3776244B2 (en) | 2006-05-17 |
JPH09505841A (en) | 1997-06-10 |
HU219851B (en) | 2001-08-28 |
CA2173105A1 (en) | 1995-04-20 |
CN1082999C (en) | 2002-04-17 |
ATE361355T1 (en) | 2007-05-15 |
EP0723579B1 (en) | 2007-05-02 |
DE69434962T2 (en) | 2008-01-17 |
CN1137289A (en) | 1996-12-04 |
DE69434962D1 (en) | 2007-06-14 |
ES2287931T3 (en) | 2007-12-16 |
TW306931B (en) | 1997-06-01 |
JPH11246895A (en) | 1999-09-14 |
AU8079794A (en) | 1995-05-04 |
EP0723579A1 (en) | 1996-07-31 |
HUT75984A (en) | 1997-05-28 |
CA2173105C (en) | 2003-05-27 |
HU9600960D0 (en) | 1996-06-28 |
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