WO1995008620B1 - Recovery of insoluble biosynthetic products - Google Patents
Recovery of insoluble biosynthetic productsInfo
- Publication number
- WO1995008620B1 WO1995008620B1 PCT/US1994/010397 US9410397W WO9508620B1 WO 1995008620 B1 WO1995008620 B1 WO 1995008620B1 US 9410397 W US9410397 W US 9410397W WO 9508620 B1 WO9508620 B1 WO 9508620B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- solid phase
- acid
- indigo
- insoluble
- cell mass
- Prior art date
Links
- 238000011084 recovery Methods 0.000 title claims abstract 5
- 230000001851 biosynthetic Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 22
- 230000004151 fermentation Effects 0.000 claims abstract 16
- 238000000855 fermentation Methods 0.000 claims abstract 16
- 239000007790 solid phase Substances 0.000 claims abstract 15
- 239000003513 alkali Substances 0.000 claims abstract 9
- 239000002253 acid Substances 0.000 claims abstract 8
- 230000002255 enzymatic Effects 0.000 claims abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims 12
- 229940097275 Indigo Drugs 0.000 claims 9
- 241001062009 Indigofera Species 0.000 claims 9
- 235000000177 Indigofera tinctoria Nutrition 0.000 claims 9
- KWYUFKZDYYNOTN-UHFFFAOYSA-M potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N Perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 3
- KDFQYGBJUYYWDJ-UHFFFAOYSA-N azane;sodium Chemical compound N.[Na] KDFQYGBJUYYWDJ-UHFFFAOYSA-N 0.000 claims 3
- GRYLNZFGIOXLOG-UHFFFAOYSA-N nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims 2
- 102000016911 Deoxyribonucleases Human genes 0.000 claims 1
- 108010053770 Deoxyribonucleases Proteins 0.000 claims 1
- 102000033147 ERVK-25 Human genes 0.000 claims 1
- 229940088598 Enzyme Drugs 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 229920001503 Glucan Polymers 0.000 claims 1
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims 1
- 102000016943 Muramidase Human genes 0.000 claims 1
- 108010014251 Muramidase Proteins 0.000 claims 1
- 108091005771 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 229960000274 lysozyme Drugs 0.000 claims 1
- 239000004325 lysozyme Substances 0.000 claims 1
- 235000010335 lysozyme Nutrition 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- 239000012071 phase Substances 0.000 abstract 1
- 238000005063 solubilization Methods 0.000 abstract 1
Abstract
There is disclosed a process for the recovery of an insoluble fermentation product from a solid phase cell mass, the process comprising treating the fermentation broth to solubilize the cell mass and separating by known methods the liquid phase from the insoluble (product-containing) phase. The solubilization of the cell mass may be carried out by one or more treatments with an alkali or acid compound or by enzymatic treatment of the cell mass to lyze the cells.
Claims
1. A recovery process useful for recovering an insoluble fermentation product from an aqueous fermentation broth comprising a first solid phase containing the insoluble fermentation product and a second solid phase containing cell mass, the process comprising: a) treating the aqueous fermentation broth one or more times with either an appropriate alkali or an appropriate acid at a temperature over room temperature for a period of time sufficient to solubilize at least about 20% of cell mass, resulting in an aqueous fermentation broth containing the first solid phase containing the insoluble fermentation product; and b) separating the first solid phase containing the insoluble fermentation product from the aqueous fermentation broth; and c) recovering the insoluble fermentation product from the first solid phase.
2. A process of Claim 1 wherein the alkali is selected from the group consisting of potassium hydroxide, sodium hydroxide and ammonia.
3. A process of Claim 1 wherein the acid is selected from the group consisting of phosphoric acid, sulfuric acid, hydrochloric acid, nitric acid and perchloric acid.
4. A process of Claim 1 wherein the temperature is from about 40 to 100°C.
5. A process of Claim 4 wherein the temperature is from about 90-95cC.
6. A process of Claim 1 wherein the aqueous fermentation broth is contacted with each appropriate alkali or acid for a period of from about 1 to 12 hours.
7. A process of Claim 1 further comprising resuspending one or more times the first solid phase resulting from step a) with either an appropriate alkali or an appropriate acid to solubilize residual, unsolubilized cell mass in the first solid phase prior to recovering the insoluble fermentation product.
8. A process of Claim 1 wherein the desired insoluble fermentation product is indigo, melanin or glucan.
9. A process of Claim 1 wherein the second solid phase is solubilized by enzymatic treatment of the cell mass.
10. A process of Claim 9 wherein the enzyme is selected from the group consisting of lysozyme, protease, DNase, RNase or a combination thereof.
11. A process for the recovery of indigo from an aqueous fermentation broth comprising a first solid phase containing indigo and a second solid phase containing cell mass, the process comprising: a) contacting the fermentation broth with an appropriate alkali selected from the group consisting of potassium hydroxide, sodium hydroxide and ammonia at a temperature of from about 40 to 100°C for a period of time sufficient to solubilize at least about 20% of cell mass; b) separating the first solid phase containing indigo from the aqueous broth; c) optionally resuspending the first solid phase containing indigo with either an appropriate alkali selected from the group consisting of potassium hydroxide, sodium hydroxide and ammonia or an appropriate acid selected from the group consisting of phosphoric acid, sulfuric acid, hydrochloric acid, nitric acid and perchloric acid at a temperature of from about 40 to 100°C for an appropriate period of time to solubilize residual insoluble cell mass; d) separating the first solid phase from the aqueous broth; and e) recovering the indigo from the first solid phase.
12. A process of Claim 11 wherein the alkali is sodium hydroxide and the acid is phosphoric acid.
13. A process of Claim 11 wherein the temperature is from about 90-95°C.
14. A process of Claim 13 wherein each alkali and acid step is carried out for a period of from about 1 to 12 hours.
15. A process of Claim 11 further comprising purifying the indigo product resulting from such recovery process.
16. The process of Claim 1 further comprising purifying the indigo recovered from said process.
17. The process of Claim 7 further comprising purifying the indigo recovered from said process.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7509833A JPH09502879A (en) | 1993-09-23 | 1994-09-15 | Recovery method of insoluble biosynthetic products |
| KR1019960701463A KR960705027A (en) | 1993-09-23 | 1994-09-15 | RECOVERY OF INSOLUBLE BIOSYNTHETIC PRODUCTS |
| DE69429995T DE69429995T2 (en) | 1993-09-23 | 1994-09-15 | RECOVERY OF INSOLUBLE INDIGO |
| CA002172522A CA2172522C (en) | 1993-09-23 | 1994-09-15 | Recovery of insoluble biosynthetic products |
| AT94929207T ATE213769T1 (en) | 1993-09-23 | 1994-09-15 | RECOVERY OF INSOLUBLE INDIGO |
| DK94929207T DK0720647T3 (en) | 1993-09-23 | 1994-09-15 | Extraction of insoluble indigo |
| EP94929207A EP0720647B1 (en) | 1993-09-23 | 1994-09-15 | Recovery of insoluble indigo |
| FI961338A FI119376B (en) | 1993-09-23 | 1996-03-22 | Making of insoluble indigo |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/125,997 | 1993-09-23 | ||
| US08/125,997 US5492819A (en) | 1993-09-23 | 1993-09-23 | Recovery of insoluble biosynthetic products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1995008620A1 WO1995008620A1 (en) | 1995-03-30 |
| WO1995008620B1 true WO1995008620B1 (en) | 1995-04-20 |
Family
ID=22422458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1994/010397 WO1995008620A1 (en) | 1993-09-23 | 1994-09-15 | Recovery of insoluble biosynthetic products |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5492819A (en) |
| EP (1) | EP0720647B1 (en) |
| JP (1) | JPH09502879A (en) |
| KR (1) | KR960705027A (en) |
| CN (1) | CN1131436A (en) |
| AT (1) | ATE213769T1 (en) |
| CA (1) | CA2172522C (en) |
| DE (1) | DE69429995T2 (en) |
| DK (1) | DK0720647T3 (en) |
| ES (1) | ES2171466T3 (en) |
| FI (1) | FI119376B (en) |
| WO (1) | WO1995008620A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10040176B4 (en) * | 2000-08-17 | 2005-09-08 | Consortium für elektrochemische Industrie GmbH | Process for the isolation of cystine |
| CN106336685A (en) * | 2016-08-24 | 2017-01-18 | 张永成 | Method for extracting natural dye by using biological fermentation method, and applications thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4520103A (en) * | 1982-10-27 | 1985-05-28 | Amgen | Microbial production of indigo |
| JPS60196195A (en) * | 1984-03-21 | 1985-10-04 | Dainippon Seito Kk | Preparation of yeast glucan |
| US5082936A (en) * | 1984-11-28 | 1992-01-21 | Massachusetts Institute Of Technology | Glucan composition and process for preparation thereof |
| US5164485A (en) * | 1985-08-20 | 1992-11-17 | Takeda Chemical Industries, Ltd. | Modified hepatitis B virus surface antigen P31 and production thereof |
| DE3620915A1 (en) * | 1986-06-23 | 1988-01-07 | Sandoz Ag | METHOD FOR TREATING ORGANICALLY PRODUCED INDIGO |
| EP0330419B1 (en) * | 1988-02-23 | 1994-10-26 | MITSUI TOATSU CHEMICALS, Inc. | Process for the preparation of indigo compound |
| ATE117376T1 (en) * | 1988-10-03 | 1995-02-15 | Biosource Genetics Corp | PRODUCTION OF MELANIN. |
| SE8901687D0 (en) * | 1989-05-11 | 1989-05-11 | Alfa Laval Agri Int | FIBRONECTIN BINDING PROTEIN AS WELL AS IT'S PREPARATION |
| US5116997A (en) * | 1991-04-15 | 1992-05-26 | Basf Aktiengesellschaft | Purification of indigo |
-
1993
- 1993-09-23 US US08/125,997 patent/US5492819A/en not_active Expired - Lifetime
-
1994
- 1994-09-15 ES ES94929207T patent/ES2171466T3/en not_active Expired - Lifetime
- 1994-09-15 KR KR1019960701463A patent/KR960705027A/en not_active IP Right Cessation
- 1994-09-15 CN CN94193462A patent/CN1131436A/en active Pending
- 1994-09-15 DK DK94929207T patent/DK0720647T3/en active
- 1994-09-15 JP JP7509833A patent/JPH09502879A/en active Pending
- 1994-09-15 EP EP94929207A patent/EP0720647B1/en not_active Expired - Lifetime
- 1994-09-15 AT AT94929207T patent/ATE213769T1/en active
- 1994-09-15 WO PCT/US1994/010397 patent/WO1995008620A1/en active IP Right Grant
- 1994-09-15 DE DE69429995T patent/DE69429995T2/en not_active Expired - Lifetime
- 1994-09-15 CA CA002172522A patent/CA2172522C/en not_active Expired - Fee Related
-
1996
- 1996-03-22 FI FI961338A patent/FI119376B/en not_active IP Right Cessation
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