WO1995004138A1 - Matieres cellulosiques delignifiees servant a ameliorer les processus industriels de fermentation alcoolique - Google Patents

Matieres cellulosiques delignifiees servant a ameliorer les processus industriels de fermentation alcoolique Download PDF

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Publication number
WO1995004138A1
WO1995004138A1 PCT/GR1994/000018 GR9400018W WO9504138A1 WO 1995004138 A1 WO1995004138 A1 WO 1995004138A1 GR 9400018 W GR9400018 W GR 9400018W WO 9504138 A1 WO9504138 A1 WO 9504138A1
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WO
WIPO (PCT)
Prior art keywords
fermentation
cellulosic material
delignified
delignified cellulosic
relation
Prior art date
Application number
PCT/GR1994/000018
Other languages
English (en)
Inventor
Athanasios Koutinas
Lazaros Iconomou
Eustratia Bardi
Original Assignee
Athanasios Koutinas
Lazaros Iconomou
Eustratia Bardi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athanasios Koutinas, Lazaros Iconomou, Eustratia Bardi filed Critical Athanasios Koutinas
Priority to EP94921072A priority Critical patent/EP0666908A1/fr
Publication of WO1995004138A1 publication Critical patent/WO1995004138A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C12N11/12—Cellulose or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C12C11/09—Fermentation with immobilised yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G1/00—Preparation of wine or sparkling wine
    • C12G1/02—Preparation of must from grapes; Must treatment and fermentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • This invention aims to the use of delignified cellulosic materials as catalyst of alcoholic fermentation and supports for immobilization of the yeast cells.
  • the biocatalysts prepared by immobilisation of yeast on delignitied cellulosic materials are suitable for low temperature alcoholic Krutation as well as for continuous processes.
  • the ethanol production yield is higher than the case of without of it. It is supported to lower residual sugar analyzed and higher final ethanol concentration obtained and the treatment with hot sodium hydroxide solution increases the possibility cells going through and so accommodate their immobilization. That in relation with low prise of the support and its abundance in nature make this biocatalyst attractive in the ethanol production as well as in wine making and beer production.
  • the particles form of the support give the possibility for fermentations using feed batch bioreactors and separation of biocatalyst employing centrifuge separators or separation after removal of the supernatant liquid. The last will be achieved only in the case which it is possible.
  • the form of particles of the support permits the use of a fluidized bed bioreactor. These bioreactors can be used in the potable alcohol production, grade - fuel alcohol production, wine making and beer production.
  • Biocatalyst prepared by immobilization of the psychrophile and alcohol resistant strain VISANTO - 1 on delignified cellulosic material leads to the production of beer and wine at 0-4 oC with a satisfactory fermentation rate and in a fermentation near of equal to that of natural fermentation of must at room temperature. This biocatalyst give an important increase of the fermentation rate. The last leads to a reduction of cost and more safe fermentation at low temperatures.
  • Two conical flasks of 11 contain each 300 ml synthetic media containing glucose 40% [ initial oBe density 19.4 ], 0.5% MgSO 4 and 0.4 yeast extract in it were also diluted 0.1 KH 2 PO 4 as well as 0.1% (NH 4 ) 2 SO 4 . In this liquid was spread baker's yeast so that to obtain on cell concentration 20 g/l. In the first flask was not added any amount of delignified cellulosic material, while in the second was added so that the concentration of this material in a dry weight basis be equal to 80 g/l. However, since delignified cellulosic material contains 65% humidity, it causes a reduction of glucose and cell concentration.
  • the Bioreactor was pumped for 65 days with must without any substantial decrease in the productivity. Products obtained every day had a 10.7 -11.2% v/v alcohol concentrations, residual sugar 25 - 40 g/l and volatile acidity 0.15 - 0.35 g CH 3 OOH/ l

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procédé d'extraction de la lignine des matières cellulosiques. Ces matières peuvent servir de catalyseurs dans la fermentation associée à la fabrication d'alcools, à l'aide de solutions de mélasse et de glucose à titre de matières premières. Il s'avère que, dans le cas des procédés utilisés et des résultats obtenus, les matières exemptes de lignine assurent une augmentation de la vitesse de fermentation alcoolique et du degré alcoolique final, par rapport à la fermentation réalisée en l'absence de ces matières. En outre, on propose l'utilisation de matières exemptes de lignine dans l'immobilisation de cellules de levure de fermentation. On a mis en évidence que les biocatalyseurs produits de cette façon sont utilisables dans la fabrication du vin et de la bière à basse température (de 0 à 5 °C) avec des périodes de fermentation satisfaisantes, mais aussi dans la fermentation d'un grand nombre de lots de fermentation à des températures normales et avec de courtes périodes de fermentation. Enfin, il s'avère que le biocatalyseur obtenu par immobilisation de cellules dans les matières cellulosiques exemptes de lignine est approprié pour la fabrication du vin et de la bière dans un réacteur à fonctionnement continu, ce qui a été démontré par le fait que le rendement du réacteur n'a pas baissé pendant les 65 premiers jours d'exploitation.
PCT/GR1994/000018 1993-07-27 1994-07-14 Matieres cellulosiques delignifiees servant a ameliorer les processus industriels de fermentation alcoolique WO1995004138A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP94921072A EP0666908A1 (fr) 1993-07-27 1994-07-14 Matieres cellulosiques delignifiees servant a ameliorer les processus industriels de fermentation alcoolique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GR93100320 1993-07-27
GR930100320 1993-07-27

Publications (1)

Publication Number Publication Date
WO1995004138A1 true WO1995004138A1 (fr) 1995-02-09

Family

ID=10941369

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GR1994/000018 WO1995004138A1 (fr) 1993-07-27 1994-07-14 Matieres cellulosiques delignifiees servant a ameliorer les processus industriels de fermentation alcoolique

Country Status (2)

Country Link
EP (1) EP0666908A1 (fr)
WO (1) WO1995004138A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002068578A1 (fr) * 2001-02-23 2002-09-06 University Of Georgia Research Foundation, Inc. Support inerte fibreux destine a la fermentation de biere et vin clair
CN106636221A (zh) * 2016-09-30 2017-05-10 广西罗城科潮基业科技发展有限公司 一种以淀粉质原料生产酒精的无酸发酵方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2206329A1 (en) * 1972-11-15 1974-06-07 Aquitaine Petrole Enzyme-cellulose complexes - esp. suitable for milk coagulation for cheeses, utilisable as large particles in enzymatic fixed or mobile beds
WO1981000576A1 (fr) * 1979-08-23 1981-03-05 B Haegerdal Enzyme(s) et micro-organisme(s) immobilise(s) et leur production
WO1981001714A1 (fr) * 1979-12-06 1981-06-25 Purdue Research Foundation Micro-organismes myceliens sous forme de comprimes
US4649113A (en) * 1983-12-28 1987-03-10 The United States Of America As Represented By The Secretary Of Agriculture Alkaline peroxide treatment of nonwoody lignocellulosics
DE3824743C1 (en) * 1988-07-21 1989-09-21 Otto Dr.Rer.Nat. Moebus Process for the production of biotechnological products by solid fermentation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2206329A1 (en) * 1972-11-15 1974-06-07 Aquitaine Petrole Enzyme-cellulose complexes - esp. suitable for milk coagulation for cheeses, utilisable as large particles in enzymatic fixed or mobile beds
WO1981000576A1 (fr) * 1979-08-23 1981-03-05 B Haegerdal Enzyme(s) et micro-organisme(s) immobilise(s) et leur production
WO1981001714A1 (fr) * 1979-12-06 1981-06-25 Purdue Research Foundation Micro-organismes myceliens sous forme de comprimes
US4649113A (en) * 1983-12-28 1987-03-10 The United States Of America As Represented By The Secretary Of Agriculture Alkaline peroxide treatment of nonwoody lignocellulosics
DE3824743C1 (en) * 1988-07-21 1989-09-21 Otto Dr.Rer.Nat. Moebus Process for the production of biotechnological products by solid fermentation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
M. YASUHISA: "Developments in alcohol manufacturing technology.", INTERNATIONAL JOURNAL OF SOLAR ENERGY, vol. 7, no. 2, 1989, LONDON GB, pages 93 - 109 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002068578A1 (fr) * 2001-02-23 2002-09-06 University Of Georgia Research Foundation, Inc. Support inerte fibreux destine a la fermentation de biere et vin clair
CN106636221A (zh) * 2016-09-30 2017-05-10 广西罗城科潮基业科技发展有限公司 一种以淀粉质原料生产酒精的无酸发酵方法

Also Published As

Publication number Publication date
EP0666908A1 (fr) 1995-08-16

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