WO1995001998B1 - Protein-enriched thermoplastics - Google Patents
Protein-enriched thermoplasticsInfo
- Publication number
- WO1995001998B1 WO1995001998B1 PCT/US1994/007776 US9407776W WO9501998B1 WO 1995001998 B1 WO1995001998 B1 WO 1995001998B1 US 9407776 W US9407776 W US 9407776W WO 9501998 B1 WO9501998 B1 WO 9501998B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- polypeptide
- thermoplastic
- melt
- interdispersed
- composition
- Prior art date
Links
- 229920001169 thermoplastic Polymers 0.000 title claims abstract 14
- 239000004416 thermosoftening plastic Substances 0.000 title claims abstract 14
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 12
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 12
- 239000000203 mixture Substances 0.000 claims abstract 12
- 239000004033 plastic Substances 0.000 claims abstract 8
- 229920003023 plastic Polymers 0.000 claims abstract 8
- 238000010438 heat treatment Methods 0.000 claims abstract 5
- 238000000465 moulding Methods 0.000 claims abstract 5
- 238000000034 method Methods 0.000 claims abstract 2
- 229920000642 polymer Polymers 0.000 claims abstract 2
- 229920001184 polypeptide Polymers 0.000 claims 14
- 150000001413 amino acids Chemical class 0.000 claims 4
- 239000000155 melt Substances 0.000 claims 4
- 102000008186 Collagen Human genes 0.000 claims 3
- 108010035532 Collagen Proteins 0.000 claims 3
- 102000016942 Elastin Human genes 0.000 claims 3
- 108010014258 Elastin Proteins 0.000 claims 3
- 102000011782 Keratins Human genes 0.000 claims 3
- 108010076876 Keratins Proteins 0.000 claims 3
- 229920001436 collagen Polymers 0.000 claims 3
- 229960005188 collagen Drugs 0.000 claims 3
- 229920002549 elastin Polymers 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 2
- -1 polyethylene Polymers 0.000 claims 2
- 239000004698 Polyethylene (PE) Substances 0.000 claims 1
- 239000004743 Polypropylene Substances 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 239000000654 additive Substances 0.000 claims 1
- 230000000996 additive Effects 0.000 claims 1
- 230000002708 enhancing Effects 0.000 claims 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 239000004926 polymethyl methacrylate Substances 0.000 claims 1
- 229920001155 polypropylene Polymers 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 238000005266 casting Methods 0.000 abstract 2
- 238000001125 extrusion Methods 0.000 abstract 2
Abstract
Thermoplastics interdispersed with a variety of functional thermostable proteins and methods of making such thermoplastics are provided. The disclosure demonstrates that certain proteins can maintain functional integrity through exposure to plastic thermomolding. The proteins are exposed to the heating and molding/extrusion/casting process and are hence present on the formed plastic surface and at a depth below the plastic surface. The proteins contained in the disclosed compositions retain functional properties or binding specificities through the heating and molding/extrusion/casting processes. Preferred thermostable protein used in the disclosed compositions include silk-like protein polymers, particularly ProNectin®F. The disclosed methods and compositions find use in many applications where plastics containing functional thermostable proteins are desired, in particular, cell cultureware.
Claims
1. A composition comprising a biologically functional thermostable polypeptide interdispersed within a thermoplastic resulting from a melt at a temperature of at least 60° C, wherein said polypeptide is at least about 25kD, has repeating units from elastin, collagen, keratin or silk proteins and sequences intervening said repeating units having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
2. The composition of Claim 1 wherein said intervening group polypeptide is from 3 to about 60 amino acids in length.
3. The composition of Claim 1 wherein said polypeptide has a molecular weight of at least 50kD.
4. The composition of Claim 1, wherein said intervening group comprises RGD.
5. The composition of Claim 4 wherein said protein polymer is ProΝectin®F or SLPL3.0.
6. The composition of Claim 1 wherein said plastic is polystyrene, polyethylene, polypropylene, or polymethylmethacrylate.
7. The composition of Claim 1 further comprising a polypeptide thermostability enhancing additive.
8. A formed object produced by contacting a plastic with a biologically functional thermostable polypeptide, heating said plastic and polypeptide to at least 60°C for at least 15 seconds to create a melt, molding said melt to form a biologically functional thermostable polypeptide interdispersed within a thermoplastic, wherein said polypeptide is at least about 25kD, has repeating units from elastin, collagen, keratin or silk proteins and sequences intervening said repeating units having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
9. A method for producing a biologically functional thermostable polypeptide interdispersed within a thermoplastic, said method comprising: forming a mixture of a plastic and said thermostable polypeptide, heating said thermoplastic and said polypeptide to create a melt, molding said melt into a predetermined form, whereby a biologically functional thermostable polypeptide is interdispersed within a thermoplastic, wherein said polypeptide is at least about 25kD, has repeating units from elastin, collagen, keratin or silk proteins and sequences intervening said repeating units having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
10. A method for producing biologically functional ProNectin®F interdispersed within a thermoplastic, said method comprising: forming a mixture of a thermoplastic and ProNectin®F, heating said thermoplastic and said ProNectin®F to create a melt, molding said melt into a predetermined form, whereby biologically functional ProNectin®F is interdispersed within said thermoplastic.
STATEMENT UNDER ARTICLE 19
In response to the International Search Report for the above captioned PCT application, applicants amend the claims by submitting replacement sheets 69-70, enclosed. Claims 1-10 have been amended to limit the claimed thermoplastics to ones which are produced by a particular process. This limitation further distinguishes the claimed thermoplastics from those products disclosed in the references listed in the search report.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU73594/94A AU7359494A (en) | 1993-07-09 | 1994-07-08 | Protein-enriched thermoplastics |
| US08/587,333 US5808012A (en) | 1993-07-09 | 1996-01-16 | Protein-enriched thermoplastics |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/089,862 US5723588A (en) | 1986-11-04 | 1993-07-09 | Protein-enriched thermoplastics |
| US08/089,862 | 1993-07-09 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1995001998A2 WO1995001998A2 (en) | 1995-01-19 |
| WO1995001998A3 WO1995001998A3 (en) | 1995-01-19 |
| WO1995001998B1 true WO1995001998B1 (en) | 1995-03-09 |
Family
ID=22219947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1994/007776 WO1995001998A2 (en) | 1993-07-09 | 1994-07-08 | Protein-enriched thermoplastics |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US5723588A (en) |
| AU (1) | AU7359494A (en) |
| WO (1) | WO1995001998A2 (en) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG49993A1 (en) * | 1995-12-28 | 1998-06-15 | Fujirebio Kk | Fused dna sequence fused protein expressed from said fused dna sequence and method for expressing said fused protein |
| US6177282B1 (en) * | 1997-08-12 | 2001-01-23 | Mcintyre John A. | Antigens embedded in thermoplastic |
| US7018798B2 (en) | 1997-08-12 | 2006-03-28 | Embedded Concepts, Llc | Nucleic acid antigens embedded in thermoplastic |
| US20020156434A1 (en) * | 1998-03-13 | 2002-10-24 | Minimed Inc. | Stabilizing catheter for protein drug delivery |
| WO2001006045A1 (en) * | 1999-07-14 | 2001-01-25 | Kaneka Corporation | Regenerated collagen fiber with excellent heat resistance |
| US20010053931A1 (en) * | 1999-11-24 | 2001-12-20 | Salvatore J. Abbruzzese | Thin-layered, endovascular silk-covered stent device and method of manufacture thereof |
| JP3993855B2 (en) | 2001-11-01 | 2007-10-17 | スパイン・ウェイブ・インコーポレーテッド | Device for spinal disc recovery |
| AU2002350026A1 (en) * | 2001-11-01 | 2003-05-12 | Lawrence M. Boyd | System and method for the pretreatment of the endplates of an intervertebral disc |
| US20090238811A1 (en) * | 2002-09-09 | 2009-09-24 | Mcdaniel C Steven | Enzymatic Antimicrobial and Antifouling Coatings and Polymeric Materials |
| US20050142094A1 (en) * | 2003-03-12 | 2005-06-30 | Manoj Kumar | Use of repeat sequence protein polymers in personal care compositions |
| US7297678B2 (en) * | 2003-03-12 | 2007-11-20 | Genencor International, Inc. | Use of repeat sequence protein polymers in personal care compositions |
| US7846137B2 (en) * | 2003-12-26 | 2010-12-07 | Medtronic Minimed, Inc. | Modular catheter system |
| WO2005063800A2 (en) * | 2003-12-31 | 2005-07-14 | F. Hoffmann-La Roche Ag | Peptide synthesis using decanting filter |
| US20050148703A1 (en) * | 2003-12-31 | 2005-07-07 | Barone Justin R. | Polymer composites containing keratin |
| US20050148928A1 (en) * | 2003-12-31 | 2005-07-07 | Medtronic Minimed, Inc. | Multiple layer tubing and method of making same |
| JP4650798B2 (en) | 2004-04-19 | 2011-03-16 | デンカ生研株式会社 | Virus production method |
| US7837733B2 (en) | 2004-06-29 | 2010-11-23 | Spine Wave, Inc. | Percutaneous methods for injecting a curable biomaterial into an intervertebral space |
| BE1016177A6 (en) * | 2004-09-03 | 2006-04-04 | Resilux | METHOD FOR MANUFACTURING HYDROFOBE POLYMERS |
| US8642307B2 (en) * | 2006-05-25 | 2014-02-04 | Nalge Nunc International Corporation | Cell culture surface chemistries |
| US8497126B2 (en) * | 2006-06-30 | 2013-07-30 | Corning Incorporated | Method of making enhanced cell growth surface |
| KR100829213B1 (en) * | 2006-12-06 | 2008-05-14 | 송승민 | Mold of recipient block and usage thereof |
| US20100310630A1 (en) | 2007-04-27 | 2010-12-09 | Technische Universitat Braunschweig | Coated surface for cell culture |
| US10821085B2 (en) | 2010-12-07 | 2020-11-03 | Kimberly-Clark Worldwide, Inc. | Wipe coated with a botanical composition having antimicrobial properties |
| US9832993B2 (en) | 2010-12-07 | 2017-12-05 | Kimberly-Clark Worldwide, Inc. | Melt processed antimicrobial composition |
| US8445032B2 (en) | 2010-12-07 | 2013-05-21 | Kimberly-Clark Worldwide, Inc. | Melt-blended protein composition |
| US8524264B2 (en) | 2010-12-07 | 2013-09-03 | Kimberly-Clark Worldwide, Inc. | Protein stabilized antimicrobial composition formed by melt processing |
| US9648874B2 (en) | 2010-12-07 | 2017-05-16 | Kimberly-Clark Worldwide, Inc. | Natural, multiple use and re-use, user saturated wipes |
| US9149045B2 (en) | 2010-12-07 | 2015-10-06 | Kimberly-Clark Worldwide, Inc. | Wipe coated with a botanical emulsion having antimicrobial properties |
| US9700425B1 (en) | 2011-03-20 | 2017-07-11 | Nuvasive, Inc. | Vertebral body replacement and insertion methods |
| US8574628B2 (en) | 2011-12-19 | 2013-11-05 | Kimberly-Clark Worldwide, Inc. | Natural, multiple release and re-use compositions |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57108010A (en) * | 1980-12-25 | 1982-07-05 | Japan Atom Energy Res Inst | Preparation of slow-releasing composite |
| JPS58183626A (en) * | 1982-04-21 | 1983-10-26 | Nippon Oil Co Ltd | Agent for immobilizing l-asparaginase used as a remedy for leukemia |
-
1993
- 1993-07-09 US US08/089,862 patent/US5723588A/en not_active Expired - Lifetime
-
1994
- 1994-07-08 WO PCT/US1994/007776 patent/WO1995001998A2/en active Application Filing
- 1994-07-08 AU AU73594/94A patent/AU7359494A/en not_active Abandoned
-
1996
- 1996-01-16 US US08/587,333 patent/US5808012A/en not_active Expired - Fee Related
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