WO1994023022A1 - AN α-GALACTOSIDASE ENZYME - Google Patents
AN α-GALACTOSIDASE ENZYME Download PDFInfo
- Publication number
- WO1994023022A1 WO1994023022A1 PCT/DK1994/000138 DK9400138W WO9423022A1 WO 1994023022 A1 WO1994023022 A1 WO 1994023022A1 DK 9400138 W DK9400138 W DK 9400138W WO 9423022 A1 WO9423022 A1 WO 9423022A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- galactosidase
- dna
- seq
- polypeptide
- cell
- Prior art date
Links
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- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
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- 206010016766 flatulence Diseases 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
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- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 108010062266 glycyl-glycyl-argininal Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 108010028188 glycyl-histidyl-serine Proteins 0.000 description 1
- 108010050848 glycylleucine Proteins 0.000 description 1
- 108010077515 glycylproline Proteins 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
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- 229930027917 kanamycin Natural products 0.000 description 1
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- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 108010057821 leucylproline Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 108010064235 lysylglycine Proteins 0.000 description 1
- 108010017391 lysylvaline Proteins 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
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- 235000014571 nuts Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 108010070409 phenylalanyl-glycyl-glycine Proteins 0.000 description 1
- 108010051242 phenylalanylserine Proteins 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 210000004896 polypeptide structure Anatomy 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 229940074439 potassium sodium tartrate Drugs 0.000 description 1
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- 108010053725 prolylvaline Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 108010048818 seryl-histidine Proteins 0.000 description 1
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- 238000012807 shake-flask culturing Methods 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
Definitions
- a preferred method of isolating a DNA construct of the invention from a cDNA or genomic library is by use of polymerase chain reaction (PCR) using degenerate oligonucleotide probes prepared on the basis of the amino acid sequence shown in SEQ ID No. 3.
- PCR polymerase chain reaction
- the PCR may be carried out using the techniques described in US Patent No. 4,683,202 or by R.K. Saiki et al. (1988).
- the ⁇ -galactosidase encoded by the DNA construct of the invention may be used for a number of purposes involving hydrolysis of ⁇ -galactosides to galactoses and sucroses.
- Fig . 5 is a HPLC chromatogram illustrating the degradation of raffinose by ⁇ -galactosidase
- pH optimum is measured by using 2mM PNP- ⁇ -galactosidase in
- the pooled ⁇ -galactosidase fractions were concentrated 10 x to obtain a protein content of about 16 mg/ml.
- the gelfiltration was performed on a Sephadex G100 (Mw 4.000-150-000) gelfiltration column equilibrated with the buffer specified above.
- the ⁇ -galactosidase which was present in the front fraction and contained 5.6 mg of protein, was subsequently analysed for purity by use of the IEF Phast system and the SDS-PAGE Phast system as described below.
- the specific activity of the front fraction was determed to 264 GALU/mg Protein as described above.
- the protein content was determined spectrophotometrically at 280 nm.
- the labelled fragment was isolated using a Sephadex G50 spun column as described by Maniatis et. al. (Maniatis et al., 1982). The probe was heat denatured for 5 min, 100°C, and then added to the hybridization mixture.
- GACACGGCCG CAACTGGGGT TTCAAAACCCA CAGACGATCG TTACGAATGG CACTAGTTTC 120
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
- ORGANISM synthetic primer ( xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 20 :
- MOLECULE TYPE DNA (genomic)
- MOLECULE TYPE DNA (genomic)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Beans For Foods Or Fodder (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE69434051T DE69434051T2 (en) | 1993-03-31 | 1994-03-30 | Alpha-galactosidase |
AT94911117T ATE278774T1 (en) | 1993-03-31 | 1994-03-30 | ALPHA-GALACTOSIDASE |
JP52156894A JP3535162B2 (en) | 1993-03-31 | 1994-03-30 | α-galactosidase enzyme |
AU63751/94A AU682028B2 (en) | 1993-03-31 | 1994-03-30 | An alpha-galactosidase enzyme |
US08/522,269 US5919690A (en) | 1993-03-31 | 1994-03-30 | α-galactosidase enzyme |
EP94911117A EP0692024B1 (en) | 1993-03-31 | 1994-03-30 | An alpha-galactosidase enzyme |
BR9406563A BR9406563A (en) | 1993-03-31 | 1994-03-30 | Construction of DNA vector for recombinant cell expression A-galactosity preparation process to produce an A-galactosity enzyme or a variant of the same use as a preparation for A-galactosity food or feed processes to prepare an enzyme-modified soy product and to convert a soy product |
US09/294,923 US6197566B1 (en) | 1993-03-31 | 1999-04-19 | α-galactosidase enzyme |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK93388A DK38893D0 (en) | 1993-03-31 | 1993-03-31 | DNA |
DK0388/93 | 1993-03-31 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/522,269 Continuation US5919690A (en) | 1993-03-31 | 1994-03-30 | α-galactosidase enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1994023022A1 true WO1994023022A1 (en) | 1994-10-13 |
Family
ID=8092943
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1994/000138 WO1994023022A1 (en) | 1993-03-31 | 1994-03-30 | AN α-GALACTOSIDASE ENZYME |
Country Status (11)
Country | Link |
---|---|
US (2) | US5919690A (en) |
EP (1) | EP0692024B1 (en) |
JP (1) | JP3535162B2 (en) |
CN (1) | CN1120351A (en) |
AT (1) | ATE278774T1 (en) |
AU (1) | AU682028B2 (en) |
BR (1) | BR9406563A (en) |
CA (1) | CA2159314A1 (en) |
DE (1) | DE69434051T2 (en) |
DK (1) | DK38893D0 (en) |
WO (1) | WO1994023022A1 (en) |
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WO1997013526A1 (en) * | 1995-10-10 | 1997-04-17 | Novo Nordisk A/S | Method of improving lipid digestion |
WO1997016555A1 (en) * | 1995-11-01 | 1997-05-09 | Nexia Biotechnologies, Inc. | Aspergillus niger beta-galactosidase gene |
WO2002018614A1 (en) * | 2000-08-30 | 2002-03-07 | Amano Enzyme Inc. | METHOD OF ELEVATING YIELD OF OLIGOSACCHARIDES CONTAINING α-GALACTOSYL AND ANTI-CANDIDA COMPOSITIONS |
WO2003046194A1 (en) * | 2001-11-26 | 2003-06-05 | Amano Enzyme Inc. | NOVEL α-GALACTOSYL-CONTAINING NON-REDUCING DISACCHARIDES AND PROCESS FOR PRODUCING THE SAME |
US6864363B2 (en) | 2000-11-08 | 2005-03-08 | University Of Georgia Research Foundation, Inc. | Dipeptidylpeptidases and methods of use |
US6875851B1 (en) * | 1999-03-05 | 2005-04-05 | University Of Georgia Research Foundation, Inc. | Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis |
US7919275B2 (en) | 1999-02-22 | 2011-04-05 | Novozymes A/S | Oxaloacetate hydrolase deficient fungal host cells |
WO2015011276A1 (en) * | 2013-07-26 | 2015-01-29 | Novozymes A/S | POLYPEPTIDES HAVING α-L-GALACTOSIDASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME |
WO2017202979A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
WO2017202966A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
WO2017202997A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity |
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US6534300B1 (en) | 1999-09-14 | 2003-03-18 | Genzyme Glycobiology Research Institute, Inc. | Methods for producing highly phosphorylated lysosomal hydrolases |
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CA1252256A (en) | 1984-12-24 | 1989-04-11 | Joel B. Barlow | Drain assembly with synthetic resin lock nut and coupling elements |
CA1339101C (en) | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
-
1993
- 1993-03-31 DK DK93388A patent/DK38893D0/en not_active Application Discontinuation
-
1994
- 1994-03-30 AU AU63751/94A patent/AU682028B2/en not_active Ceased
- 1994-03-30 AT AT94911117T patent/ATE278774T1/en not_active IP Right Cessation
- 1994-03-30 BR BR9406563A patent/BR9406563A/en not_active Application Discontinuation
- 1994-03-30 CA CA002159314A patent/CA2159314A1/en not_active Abandoned
- 1994-03-30 WO PCT/DK1994/000138 patent/WO1994023022A1/en active IP Right Grant
- 1994-03-30 EP EP94911117A patent/EP0692024B1/en not_active Expired - Lifetime
- 1994-03-30 CN CN94191638A patent/CN1120351A/en active Pending
- 1994-03-30 DE DE69434051T patent/DE69434051T2/en not_active Expired - Fee Related
- 1994-03-30 JP JP52156894A patent/JP3535162B2/en not_active Expired - Fee Related
- 1994-03-30 US US08/522,269 patent/US5919690A/en not_active Expired - Lifetime
-
1999
- 1999-04-19 US US09/294,923 patent/US6197566B1/en not_active Expired - Fee Related
Non-Patent Citations (2)
Title |
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CHEMICAL ABSTRACTS, Volume 71, No. 3, 21 July 1969 (21.07.69), (Columbus, Ohio, USA), BAHL, Om P. et al., "Glycosidases of Aspergillus Niger. I. Purification and Characterization of Alpha-and Beta-Galactosidases and Beta-N-Acetylglucosaminidase", pages 27-28, the Abstract No. 9808t, J. Biol. Chem. 1969, 244 (11), 2970-2978. * |
CHEMICAL ABSTRACTS, Volume 73, No. 5, 3 August 1970 (03.08.70), (Columbus, Ohio, USA), LEE, YUAN CHUAN et al., "Galactosidases from Aspergillus Niger", page 31, the Abstract No. 21547f, Arch. Biochem. Biophys. 1970, 138 (1), 264-271. * |
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WO1997013526A1 (en) * | 1995-10-10 | 1997-04-17 | Novo Nordisk A/S | Method of improving lipid digestion |
WO1997016555A1 (en) * | 1995-11-01 | 1997-05-09 | Nexia Biotechnologies, Inc. | Aspergillus niger beta-galactosidase gene |
US5821350A (en) * | 1995-11-01 | 1998-10-13 | Nexia Biotechnologies, Inc. | Aspergillus niger beta-galactosidase gene |
US7919275B2 (en) | 1999-02-22 | 2011-04-05 | Novozymes A/S | Oxaloacetate hydrolase deficient fungal host cells |
US6875851B1 (en) * | 1999-03-05 | 2005-04-05 | University Of Georgia Research Foundation, Inc. | Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis |
WO2002018614A1 (en) * | 2000-08-30 | 2002-03-07 | Amano Enzyme Inc. | METHOD OF ELEVATING YIELD OF OLIGOSACCHARIDES CONTAINING α-GALACTOSYL AND ANTI-CANDIDA COMPOSITIONS |
US7491518B2 (en) | 2000-08-30 | 2009-02-17 | Amano Enzyme Inc. | Method of elevating yield of oligosaccharides containing α-galactosyl and anti-candida compositions |
US6864363B2 (en) | 2000-11-08 | 2005-03-08 | University Of Georgia Research Foundation, Inc. | Dipeptidylpeptidases and methods of use |
WO2003046194A1 (en) * | 2001-11-26 | 2003-06-05 | Amano Enzyme Inc. | NOVEL α-GALACTOSYL-CONTAINING NON-REDUCING DISACCHARIDES AND PROCESS FOR PRODUCING THE SAME |
WO2015011276A1 (en) * | 2013-07-26 | 2015-01-29 | Novozymes A/S | POLYPEPTIDES HAVING α-L-GALACTOSIDASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME |
WO2017202979A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
WO2017202966A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Polypeptides having alpha-galactosidase activity and polynucleotides encoding same |
WO2017202997A1 (en) | 2016-05-24 | 2017-11-30 | Novozymes A/S | Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity |
WO2021026201A1 (en) | 2019-08-05 | 2021-02-11 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
WO2021078839A1 (en) | 2019-10-22 | 2021-04-29 | Novozymes A/S | Animal feed composition |
WO2021233936A1 (en) | 2020-05-18 | 2021-11-25 | Dsm Ip Assets B.V. | Animal feed compositions |
Also Published As
Publication number | Publication date |
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EP0692024B1 (en) | 2004-10-06 |
JP3535162B2 (en) | 2004-06-07 |
JPH08508644A (en) | 1996-09-17 |
AU682028B2 (en) | 1997-09-18 |
DE69434051T2 (en) | 2006-03-09 |
US6197566B1 (en) | 2001-03-06 |
CA2159314A1 (en) | 1994-10-13 |
AU6375194A (en) | 1994-10-24 |
US5919690A (en) | 1999-07-06 |
BR9406563A (en) | 1996-01-30 |
EP0692024A1 (en) | 1996-01-17 |
ATE278774T1 (en) | 2004-10-15 |
DE69434051D1 (en) | 2004-11-11 |
DK38893D0 (en) | 1993-03-31 |
CN1120351A (en) | 1996-04-10 |
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