WO1994013678A1 - Spirofuranone derivatives and their use in the treatment of neurodegenerative disorders - Google Patents
Spirofuranone derivatives and their use in the treatment of neurodegenerative disorders Download PDFInfo
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- WO1994013678A1 WO1994013678A1 PCT/GB1993/002500 GB9302500W WO9413678A1 WO 1994013678 A1 WO1994013678 A1 WO 1994013678A1 GB 9302500 W GB9302500 W GB 9302500W WO 9413678 A1 WO9413678 A1 WO 9413678A1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/10—Spiro-condensed systems
- C07D491/107—Spiro-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/10—Spiro-condensed systems
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
Definitions
- This invention relates to furanone derivatives useful in therapy (in particular in the treatment of neurodegenerative disorders), methods for their use, formulations including them and processes for their production.
- Japanese Patent Application 3-153690 to Yamanouchi Pharmaceutical Co
- European Patent Application 311313 and its US equivalents US Patents N°s 4,996,210 and 4,940,795, to Yamanouchi Pharmaceutical Co
- International Patent Application WO 90/15804 and European Patent Application 491562 both to Fisons Corporation disclose furanone derivatives which are indicated for use in the treatment of neurodegenerative disorders.
- Muscarinic agonists are thought to be useful in the treatment of neurodegenerative disorders including Alzheimer's disease.
- at least three subtypes of muscarinic receptor have been identified (M réelle M 2 and M 3 ) and the proportion of each varies between the different tissues of the body. It is believed that compounds which are able to stimulate M, receptors selectively will have a beneficial effect in the treatment of neurodegenerative disorders without causing unwanted side effects [see for example N M J Rupinak, Drug Development Research, 27, 77-88 (1992), in particular page 85].
- ring A is a ring of formula Al or All (in which the spiro atom is indicated by *),
- R 1 represents H or alkyl C, ⁇
- R 2 represents -(CH 2 ) n -OR 3 , -(CH 2 ) m -C(0)R 4 , fluoroalkyl C ⁇ , cyanoalkyl C w or alkyl C 1-6 substituted with a 4-7 membered optionally unsaturated heterocyclic ring;
- R 3 represents H, alkyl C, ⁇ (optionally substituted with alkoxy C ⁇ ), cycloalkyl C 4 . 7 , a 4-7 membered optionally unsaturated heterocyclic ring, phenyl (optionally substituted with halogen, hydroxy or alkyl C w ), or benzyl (optionally substituted with halogen, hydroxy or alkyl C w );
- R 4 represents OR 5 , NR 6 R 7 , N(OR 6 )R 7 or alkyl C w ;
- R s represents H, alkenyl C 2.6 , alkynyl C 2 . 6 or alkyl C 6 (optionally substituted with phenyl);
- R 6 and R 7 independently represent H or alkyl C, ⁇ ; R 8 represents H or alkyl C 1-6 ; n is 2-5; m is 1-5; p is 1 or 2; and pharmaceutically acceptable salts thereof (hereinafter referred to en bloc as "the compounds of the invention").
- a number of the compounds of the invention may have the advantage that they are more potent, have a longer duration of action, have a broader range of activity, have fewer side effects, are more stable, are more selective (in particular they may be able to stimulate the M, receptor more selectively), or have other more useful properties than the compounds of the prior art.
- compositions of formula I include acid addition salts, for example maleate. fumarate, hydrochloride and hydrobromide salts. J
- the invention includes all optical and geometric isomers of the compounds of formula I. in particular both an - (or E-) and syn- (or Z-) isomers of the carbon-nitrogen double bond, and both (R)- and (S)-stereoisomers at the 2-position of the furanone ring.
- R 1 represents methyl
- R 2 represents -(CH 2 ) n -OR 3 or -(CH 2 ) m -C(0)R 4
- R 3 represents alkyl C ⁇ (optionally substituted with alkoxy C, ⁇ ) (for example methyl, ethyl or ethoxyethyl) or phenyl
- R 4 represents 0-(alkyl C w ) or N(alkyl C 2 (for example methoxy, ethoxy, propyloxy or dimethylamino)
- R 8 represents methyl
- f) n is 2
- g) m is 1
- h) p is 1
- i) ring A is a ring of formula Al
- j) the carbon-nitrogen double bond is syn (i.e. -OR 2 is disposed adjacent to R 1 ).
- Heteroatoms in heterocyclic rings which R 2 may include and R 3 may represent include N. S and O. Particular heterocyclic rings which may be mentioned are tetrahydrofuran and 1,3-dioxan.
- R 2 is as defined above, and where necessary converting the resulting compound into a pharmaceutically acceptable salt.
- R 2 is as defined above, with a primary aliphatic amino alcohol as described by Wild et al in US Patent N° 5,120,849.
- R 2 X VI wherein R 2 is as defined above and X is a leaving group (for example halogen), in the presence of a base (for example sodium hydride).
- Compounds of formula V may be prepared from compounds of formula II by reaction with hydroxylamine.
- Compounds of formula I which are optically pure may be prepared from compounds of formula II which are optically pure (with regard to the 2-position of the furanone ring).
- Compounds of formula II which are optically pure with regard to the 2-position of the furanone ring may be prepared from the corresponding mixture of enantiomers by reaction with a sterically hindered base (for example tri-sec-butylborohydride) to give (RR) and (SS) cis-alcohols, followed by addition of a chiral group [for example (R)- or (S)-C(0)NHCH(CH 3 )(l-naphthyl)] to form diastereoisomers, followed by selective precipitation of one diastereoisomer, removal of the chiral group (for example using bis(2-methoxyethoxy)aluminium hydride) and oxidation to the ketone (for example using N-chlorosuccinimi
- the compounds of the invention may also be interconverted, for example compounds in which R 2 contains a carboxylic acid group may be converted to a corresponding ester or amide by conventional techniques.
- the compounds of the invention are useful because they possess pharmacological activ- ity in animals.
- the compounds are useful in the treatment of neurodegen ⁇ erative disorders including presenile and senile dementia (also known as Alzheimer's disease and senile dementia of the Alzheimer type respectively), Huntington's chorea, tardive dyskinesia, hyperkinesia, mania and Tourette Syndrome.
- a method of treatment of a neurodeg ⁇ enerative disorder comprising administering a therapeutically effective amount of a compound of the invention to a patient suffering s from such a disorder.
- a neurodeg ⁇ enerative disorder including presenile and senile dementia, Huntington's chorea, tardive dysl ⁇ nesia, hyperkinesia, mania and Tourette Syndrome
- administering a therapeutically effective amount of a compound of the invention to a patient suffering s from such a disorder.
- the use of the compounds of the invention as pharmaceuticals, and the use of the compounds of the invention in the manufacture of a medicament for the treatment of neurodegenerative disorders are also provided.
- Biochemical procedures for measuring affinity and estimating efficacy at brain ⁇ o muscarinic receptors are believed to be indicative of the potential utilities for these compounds.
- Muscarinic receptor subtypes are coupled preferentially to different secondary messenger ⁇ gers and ion channels.
- M receptors stimulate is phosphatidyl inositol (PI) hydrolysis while M 2 receptors inhibit adenylate cyclase.
- PI phosphatidyl inositol
- Rat brain crude membrane preparation is incubated with a radiolabelled agonist ([ 3 H]- s oxotremorine-M, Oxo-M) and various concentrations of test compound at 30°C for 60 minutes.
- the membranes are then collected by vacuum filtration on filters and receptor-bound radioactivity is determined by liquid scintillation spectroscopy.
- the affinities (Ki) of the test compound are determined from the competition binding curves using a non-linear iterative curve-fitting computer program.
- Compounds of the inven- 0 tion with a high affinity for the Oxo-M binding site with a Ki of less than l ⁇ M and preferably less than O.l ⁇ M are preferred.
- rat brain hippocampal tissue is cross sliced into 350x350 ⁇ m segments which are incubated with 3 H-myoinositol, lithium chloride, and various concentrations of test compound for 120 minutes.
- the 3 H-inositol phosphates formed are purified by ion exchange chroma- tography and the tritium content is determined by liquid scintillation spectroscopy.
- the ability of a compound to stimulate the hydrolysis of phosphatidyl inositol is compared with that of the full agonist carbachol, which is assigned a value of 100%.
- Partial agonists produce a maximal rate of inositol phosphate formation which varies, according to the compound, from 10 to 80%. Weak partial agonists and antagonists do not stimu- late the formation of inositol phosphates. Compounds of the invention with a maximal rate of inositol phosphate formation of greater than 15% are preferred.
- Partial agonists identified in the above assays may be tested for any selectivity for M, versus M 2 receptors.
- a measure of M 2 -receptor mediated inhibition of adenylate cyclase in rat heart membranes can be obtained according to procedures described by F J Ehlert et al, J Pharmacol Exp Ther, 228:23-29 (1987).
- Some of the compounds may possess muscarinic antagonist properties and thus may be useful as antisecretory agents in the management of peptic ulcers and acute rhinitis, or in the treatment of motion sickness and Parkinson's disease.
- the compounds of the invention may be administered by any convenient route, for example orally, parenterally or rectally.
- the daily dose required will of course vary with the particular compound used, the particular condition being treated and with the sever ⁇ ity of that condition. However, in general a total daily dose of from about 0.1 to lOmg/kg of body weight, and preferably about 0.1 to lmg/kg, is suitable, administered from 1 to 4 times a day.
- the compound of the invention will generally be administered in the form of a suitable pharmaceutical formulation.
- a pharmaceutical formulation including preferably less than 50% by weight of a compound of the invention in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier.
- the pharmaceutical formulation is preferably in unit dose form.
- Such forms include solid dosage forms, for example tablets, pills, capsules, pow- ders, granules, and suppositories for oral, parenteral or rectal administration; and liquid dosage forms, for example sterile parenteral solutions or suspensions, suitably flavoured syrups, flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil and peanut oil, and elixirs and similar pharmaceutical vehicles.
- Solid formulations may be prepared by mixing the active ingredient with pharmaceutical carriers, for example conventional tabletting ingredients such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate, gums and other diluents, for example water, to form a homogeneous preformulation formulation ⁇ o in which the active ingredient is uniformly dispersed so that it may be readily subdivided into equally effective unit dosage forms containing typically from 0.1 to about 500mg of the active ingredient.
- the solid dosage forms may be coated or otherwise compounded to prolong the action of the formulation.
- a peripherally acting cholinergic antagonist such as N-methylscopolamine, N-methylatropine, propantheline, methanth- eline or glycopyrrolate.
- the methanol was removed in vacuo and the residue was taken up in deionized water.
- the pH was adjusted to 6.5 with saturated aqueous Na 2 C0 3 .
- the aqueous layer was washed with a small volume of CHC1 3 , and this was extracted twice with water.
- the combined aqueous layers were evaporated in vacuo at 35°C.
- the residue was then treated with four portions of absolute ethanol, followed by anhydrous CH 2 C1 2 (with evaporation in vacuo after each addition) to give 7.5g of crude product.
- the crude product was purified by flash chromatography on silica using an ammoniated 10-20% CH 3 OH/CHCl 3 gradient. An NMR of recovered starting material showed that the acid was enriched in syn to a 3/2 ratio.
- the maleate salt of the amide was obtained ⁇ o from ethyl acetate/Et 2 0, giving 0.3 lg (84%) of white solid.
- the subtitle compound was prepared by a modification of the process disclosed in Ger- 0 man Patent Application N° 3615473 as follows. N-hydroxyphthalimide (17.0g, 0.11 mole) was combined with K 2 C0 3 (10.5g, 0.076 mole) and anhydrous N-methylpyrollidinone
- the phthalimide (16.8g, 0.076 mole) was combined with absolute ethanol (150ml) under nitrogen. Hydrazine hydrate (3.8ml, 0.076 mole) was added and the reaction was stirred and heated with an oil bath at 95°C for 30 minutes. The reaction was monitored to completion using TLC, and was cooled in an ice bath while acidifying with concentrated HC1. The reaction was heated at reflux for 15 minutes, and then cooled with an ice bath. The white solid was collected and washed with cold ethanol. The filtrate was evaporated in vacuo and three portions of absolute ethanol were added and evaporated in vacuo to remove water.
- HPLC Novapak Phenyl, CH 3 CN/H 2 0, buffer KH 2 P0 4 at Ph 2.5: Major peaks at 10.31 and 10.89 mins for anti/syn ratio of 4.8/1; peaks at 6.72 and 7.84 mins for anti/syn ratio of acid produced by hydrolysis.
- the reaction was diluted with CHC1 3 and washed with saturated aqueous sodium car ⁇ bonate.
- the aqueous layer was extracted with five portions of CHC1 3 .
- the combined organic layers were dried and stripped. Two passes through silica Prep-paks using an ammoniated toluene/acetonitrile/methanol gradient gave 0.9g, or 70% yield.
- the white solid which froze out on storing cold appeared to be hygroscopic and gave a broad melting range.
- C-sH- ⁇ N requires C 61.21, H, 7.53, N 9.52, adjusted for 0.85% H 2 0 60.69, H 7.56, N
- step (b) r2S.3S. l 'R v )-2.8-Dimethyl- l -oxa-8-azaspiro[4.5]decan-3-yl N-f l-f 1- naphthyl ethyl]carbamate s
- a solution of the product of step (a) (13.3g, 71mmoles), (R)-l-(l-naphthyl)ethyl isocyanate (16g, ⁇ lmmoles), and dibutyltin dilaurate (0.22ml, 1.3mmoles) in anhydrous toluene (350ml) was heated at reflux for 3.5 hours.
- the reac ⁇ tion mixture was cooled to ambient temperature and concentrated in vacuo.
- the resi ⁇ due was dissolved in dichloromethane (500ml), washed successively with saturated aque- ⁇ o ous sodium bicarbonate solution (100ml) and brine (100ml), dried over anhydrous magnesium sulphate, and evaporated in vacuo.
- the residue was treated with anhydrous hydrogen chloride in 2-propanol to obtain 11.3g (42%) of the subtitle compound as its hydrochloride salt.
- step (c) f2S.3SV2.8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-ol 0
- step (b) 2.1g, 5mmoles
- the reaction was heated at reflux for 2 hours, cooled in an ice bath, and treated with water dropwise until the precipitated salts formed a separable mass.
- the subtitle compound was prepared from the subtitle compound of Example 13(a) (3.18g, 17mmoles) and (S)-l-(l-naphthyl)ethyl isocyanate (3.75g, 19mmoles) using the method of Example 13(b), providing 2.53g (35%) of the hydrochloride salt.
- the subtitle compound was prepared from the compound of step (b) (0.44g, 2.4mmoles) 5 using the method of Example 13(d), giving lOOmg (18%) as the maleate salt.
- step (d) fRVqnt/-2,8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one oxime and fRV vn-2.8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one oxime
- step (c) fRVqnt/-2,8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one oxime
- Example 13(e) The title compound was prepared from the second subtitle compound of Example 13(e) (300mg, 1.51mmoles) and 2-chloro-N,N-dimethylacetamide (223mg, 1.83 mmoles) by the procedure of Example 13(f), yield 227mg (53%) following flash chromatography using ammoniated chloroform/methanol [92:8].
- Example 14(d) The title compound was prepared from the second subtitle compound of Example 14(d) (300mg, 1.51mmoles) and 2-chloro-N,N-dimethylacetamide (226mg, 1.85mmoles) by the procedure of Example 13(f), yield 310mg (73%) following flash chromatography using ammoniated chloroform/methanol [95:5]-[90:10].
- Example 13(e) The title compound was prepared from the first subtitle compound of Example 13(e) (300mg, 1.51mmoles) and methyl chloroacetate (209mg, 1.92 mmoles) by the procedure of Example 13(f), yield 317mg (77%).
- Example 14(d) The title compound was prepared from the first subtitle compound of Example 14(d) - (200mg, l.OOmmoles) and methyl chloroacetate (130mg, 1.20mmoles) by the procedure 5 of Example 13(f), yield 90mg (25%) as its oxalate salt.
- Example 19 fSVwn-2.8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one.
- O-fmethoxycarbonylmethvO oxime The title compound was prepared from the second subtitle compound of Example 13(e) (248mg, 1.25mmoles) and methyl chloroacetate (166mg, 1.53mmoles) by the procedure of Example 13(f), yield 159mg (47%) following flash chromatography using ammoniated s chloroform/methanol [92:8].
- Example 22 fR nri-2.8-Dimethyl-l-oxa-8-azaspiro[4,5]decan-3-one.
- O- ⁇ -methoxyethvO oxime The title compound was prepared from the first subtitle compound of Example 14(d) (301mg, 1.52mmoles) and 2-bromoethyl methyl ether (265mg, 1.91mmoles) using the method of Example 13(f), yield 290mg (75%) following flash chromatography using ammoniated chloroform/methanol [94:6].
- Example 23 fS -5v -2,8-Dimethyl-l-oxa-8-azaspiro[4,5]decan-3-one, O-f2-methoxyethv0 oxime
- the title compound was prepared from the second subtitle compound of Example 13(e) (300mg, 1.51mmoles) and 2-bromoethyl methyl ether (268mg, 1.93mmoles) using the method of Example 13(f), yield 225mg (58%) following flash chromatography using ammoniated chloroform/methanol [95:5].
- Example 24 fRV vn-2.8-Dimethyl-l-oxa-8-azaspiro[4,5]decan-3-one, O-(2-methoxyethy0 oxime
- the title compound was prepared from the second subtitle compound of Example 14(d) (301mg, 1.52mmoles) and 2-bromoethyl methyl ether (270mg, 1.94mmoles) using the method of Example 13(f), yield 343mg (88%) following flash chromatography using ammoniated chloroform/methanol [95:5]. m.p. 85-88°C (oxalate salt, ethyl acetate) MS (CI): 257 [M+H] +
- the title compound was prepared from the second subtitle compound of Example 13(e) (300mg, 1.51mmoles) and 2-bromoethyl ethyl ether (330mg, 1.94mmoles) using the method of Example 13(f), yield 230mg (56%) following flash chromatography using ammoniated chloroform/methanol [95:5], followed by ammoniated ethyl acetate/hex- ane/methanol [6:6:1].
- the title compound was prepared from the second subtitle compound of Example 14(d) (350mg, 1.76 mmoles) and 2-bromoethyl methyl ether (385mg, 2.26mmoles) using the method of Example 13(f), yield 230mg (48%) following flash chromatography using amm ⁇ oniated chloroform methanol [95:5], followed by ammoniated ethyl acetate/hexane/methanol [6:6:1].
- the title compound was prepared from 2,8-dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one hydrochloride ( l.Og, 4.55mmol) and cyanomethoxyamine hydrochloride (0.62g, 4.57mmol) according to the methods of Example 4, providing 850mg (79%) of a 3:1 anti/syn mixture of isomers following purification by HPLC (Delta Prep 4000) eluting with ammoniated chloroform/methanol [98:2] to [95:5].
- Example 30 o flnri-2.8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one O-fethoxycarbonylmethv ⁇ oxime
- the title compound was prepared from a racemic mixture of the first title compound of Example 13(e) and the first title compound of Example 14(d) (500mg, 2.52mmol) and ethyl bromoacetate (530mg, 3.17mmol) using the method of Example 13(f), providing 420mg (58%) following flash chromatography eluting with ammoniated chloroform meth- 5 anol [95:5].
- Example 32 fln ⁇ -8-Methyl-l-oxa-8-azaspiro[4.5]decan-3-one 0-fmethoxycarbonylmethvO oxime ⁇ o ⁇ nri-8-Methyl-l-oxa-8-azaspiro[4.5]decan-3-one oxime was first prepared from 8-methyl- l-oxa-8-azaspiro[4.5]decan-3-one oxime (the compound of Example 3, International Patent Application WO 90/15804) using HPLC.
- Example 34 ⁇ rct.-2,8-Dimethyl-l-oxa-8-azaspiro[4.5]decan-3-one.
- Q-d.3-dioxoian-2-ylmethyl) oxime The title compound was prepared from a racemic mixture of the first title compound of Example 13(e) and the first title compound of Example 14(d) (400mg) and 2-brom- omethyl-l,3-dioxolane using the method of Example 13(f), providing 270mg (47%) following flash chromatography eluting with ammoniated chloroform/methanol [95:5].
- the title compound was prepared from a racemic mixture of the first title compound of Example 13(e) and the first title compound of Example 14(d) (400mg) and 2-brom- omethyl-tetrahydrofuran using the method of Example 13(f), providing 130mg (24%) following flash chromatography eluting with ammoniated chloroform/methanol [95:5].
- step (a) Sodium hydride (60% dispersion in oil, 8.8g, 221mmol) was washed with hexane, sus ⁇ pended in DMF (80ml), cooled on ice and ethyl lactate (26. lg, 221mmol) added cau ⁇ tiously keeping the foaming under control. The mixture was stirred at room tempera- ture for 1 hour and the product of step (a) (8g) was added slowly to the resulting dark solution. The reaction was stirred at room temperature overnight.
- reaction mixture was then diluted with ether (200ml) and acidified with Hcl satu ⁇ rated ethanol. Hexane (100ml) was added and the top solvent layer was separated. The bottom layer, which contained most of the subtitle compound, was washed with ether and the combined washings were diluted with hexane, precipitating a brown oil which also contained some of the subtitle compound.
- reaction mixture was basified with 25% NaOH, extracted with 2 portions of CHC1 3 , the extracts were dried (MgS0 4 ), the solvent was evaporated and the residue was flash chromatographed through NH 3 -deactivated silica eluting with CHClj/MeOH [20:1] giving 0.49g of a yellow solid.
- reaction mixture was concentrated, and the residue was partitioned between 20ml saturated aqueous Na 2 C0 3 solution and 20ml CHC1 3 .
- the layers were separated, and the aqueous layer was extracted with CHC1 3 (3x20ml).
- the organic layers were then combined, dried (MgS0 4 ), and concentrated, yielding 1.14g of crude product.
- the crude product was purified using HPLC using two high resolution columns, eluting with toluene/ethyl acetate/ammoniated methanol [84:8:8], to give 520mg of the E-isomer, followed by 300mg of mixed fractions.
- the 2,4,6-trinitrobenzenesulphonic acid salt was made of the E-isomer, and, after recrystallization from isopropanol, yielded 630mg of the pure salt (m.p. 154-157°C).
- Further purification of the mixed fractions obtained from the HPLC purification gave the Z-isomer as a tan oil (20mg) [m.p. 167-171°C (2,4,6-trinitrobenzenesulphonic acid salt, isopropanol)].
- step (a) To a suspension of 60% NaH oil suspension (0.065g, 1.63mmol) in dry DMF (6ml) under nitrogen was added the E-isomer of step (a) (0.32g, 1.63mmol) and the mixture was then stirred for 0.5 hour. The resulting solution was cooled to -5°C with an ice- methanol bath and N,N-dimethyl chloroacetamide (0.198g, 1.63mmol) was added in one portion. The reaction mixture was warmed to room temperature, diluted with ether, acidified with Hcl saturated EtOH, and diluted with hexane. The liquid was decanted and the precipitated gum was washed with ether. The residue was partitioned between chloroform and saturated sodium carbonate.
- Example 37(b) Following the method of Example 37(b), the title compound was prepared from the Z- isomer of Example 37(a) (lOOmg, 0.51mmol). The reaction mixture was partitioned between CHC1 3 and water. The aqueous layer was extracted with CHC1 3 , the organic extracts were dried (MgS0 4 ), the solvent was evaporated and residual DMF was removed under high vacuum. Chromatographic purification afforded 37mg of the title compound which was recrystallized from cyclohexane.
Abstract
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950702268A KR950704322A (en) | 1992-12-05 | 1993-12-06 | Spirofuranone Derivatives and Their Use in the Treatment of Neurodegenerative Disorders |
AU56553/94A AU5655394A (en) | 1992-12-05 | 1993-12-06 | Spirofuranone derivatives and their use in the treatment of neurodegenerative disorders |
EP94902038A EP0672046A1 (en) | 1992-12-05 | 1993-12-06 | Spirofuranone derivatives and their use in the treatment of neurodegenerative disorders |
JP6513925A JPH08503955A (en) | 1992-12-05 | 1993-12-06 | Furanone derivatives useful for treatment |
NO952194A NO952194L (en) | 1992-12-05 | 1995-06-02 | Spirofuranone derivatives and their use in the treatment of neurodegenerative diseases |
FI952733A FI952733A0 (en) | 1992-12-05 | 1995-06-05 | Spirofuranone derivatives and their use in neurodegenerative disorders |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB929225497A GB9225497D0 (en) | 1992-12-05 | 1992-12-05 | Pharmacologically active compounds |
GB929225498A GB9225498D0 (en) | 1992-12-05 | 1992-12-05 | Pharmacologically active compounds |
GB9225498.6 | 1992-12-05 | ||
GB9225497.8 | 1992-12-05 | ||
GB9316531.4 | 1993-08-10 | ||
GB939316531A GB9316531D0 (en) | 1993-08-10 | 1993-08-10 | Pharmacologically active compounds |
Publications (1)
Publication Number | Publication Date |
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WO1994013678A1 true WO1994013678A1 (en) | 1994-06-23 |
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Application Number | Title | Priority Date | Filing Date |
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PCT/GB1993/002500 WO1994013678A1 (en) | 1992-12-05 | 1993-12-06 | Spirofuranone derivatives and their use in the treatment of neurodegenerative disorders |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0672046A1 (en) |
JP (1) | JPH08503955A (en) |
KR (1) | KR950704322A (en) |
AU (1) | AU5655394A (en) |
CA (1) | CA2150947A1 (en) |
FI (1) | FI952733A0 (en) |
IL (1) | IL107881A0 (en) |
MX (1) | MX9307679A (en) |
NO (1) | NO952194L (en) |
WO (1) | WO1994013678A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6121459A (en) * | 1997-01-27 | 2000-09-19 | Warner-Lambert Company | Single pot process for producing (Z)-azabicyclo oxime ethers |
WO2001036423A1 (en) * | 1999-11-17 | 2001-05-25 | Akzo Nobel N.V. | Spiro(2h-1-benzopyran-2,4'-piperidine) derivates as glycine transport inhibitors |
WO2005068462A1 (en) * | 2004-01-08 | 2005-07-28 | F. Hoffmann-La Roche Ag | Diaza-spiropiperidine derivatives as inhibitors of transporter 1 and glycine transporter 2 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990015804A1 (en) * | 1989-06-21 | 1990-12-27 | Fisons Corporation | Novel spirofurane derivatives |
JPH03153690A (en) * | 1989-11-10 | 1991-07-01 | Yamanouchi Pharmaceut Co Ltd | Heterocyclic spiro derivative and production thereof |
EP0491562A1 (en) * | 1990-12-19 | 1992-06-24 | Fisons Corporation | Novel spirofurane derivatives |
-
1993
- 1993-12-05 IL IL10788193A patent/IL107881A0/en unknown
- 1993-12-06 CA CA002150947A patent/CA2150947A1/en not_active Abandoned
- 1993-12-06 MX MX9307679A patent/MX9307679A/en unknown
- 1993-12-06 JP JP6513925A patent/JPH08503955A/en active Pending
- 1993-12-06 EP EP94902038A patent/EP0672046A1/en not_active Withdrawn
- 1993-12-06 WO PCT/GB1993/002500 patent/WO1994013678A1/en not_active Application Discontinuation
- 1993-12-06 KR KR1019950702268A patent/KR950704322A/en not_active Application Discontinuation
- 1993-12-06 AU AU56553/94A patent/AU5655394A/en not_active Abandoned
-
1995
- 1995-06-02 NO NO952194A patent/NO952194L/en unknown
- 1995-06-05 FI FI952733A patent/FI952733A0/en not_active Application Discontinuation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990015804A1 (en) * | 1989-06-21 | 1990-12-27 | Fisons Corporation | Novel spirofurane derivatives |
JPH03153690A (en) * | 1989-11-10 | 1991-07-01 | Yamanouchi Pharmaceut Co Ltd | Heterocyclic spiro derivative and production thereof |
EP0491562A1 (en) * | 1990-12-19 | 1992-06-24 | Fisons Corporation | Novel spirofurane derivatives |
Non-Patent Citations (1)
Title |
---|
CHEMICAL ABSTRACTS, vol. 115, no. 19, 11 November 1991, Columbus, Ohio, US; abstract no. 207873a, S. TSUKAMOTO ET AL: "Preparation of heterocyclic spiro derivatives as muscarinic acetylcholine receptor M1 agonists" page 1010; * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6121459A (en) * | 1997-01-27 | 2000-09-19 | Warner-Lambert Company | Single pot process for producing (Z)-azabicyclo oxime ethers |
WO2001036423A1 (en) * | 1999-11-17 | 2001-05-25 | Akzo Nobel N.V. | Spiro(2h-1-benzopyran-2,4'-piperidine) derivates as glycine transport inhibitors |
US6645973B1 (en) | 1999-11-17 | 2003-11-11 | Akzo Nobel | Spiro(2h-1-benzopyran-2,4-piperidine)derivatives as glycine transport inhibitors |
US7507824B2 (en) | 1999-11-17 | 2009-03-24 | N.V. Organon | Spiro(2H-1benzopyran-2,4′-piperidine) derivates as glycine transport inhibitors |
WO2005068462A1 (en) * | 2004-01-08 | 2005-07-28 | F. Hoffmann-La Roche Ag | Diaza-spiropiperidine derivatives as inhibitors of transporter 1 and glycine transporter 2 |
US7265126B2 (en) | 2004-01-08 | 2007-09-04 | Hoffmann-La Roche Inc. | Diaza-spiropiperidine derivatives |
CN100422183C (en) * | 2004-01-08 | 2008-10-01 | 弗·哈夫曼-拉罗切有限公司 | Diaza-spiropiperidine derivatives as inhibitors of glycine transporter 1 and glycine transporter 2 |
Also Published As
Publication number | Publication date |
---|---|
EP0672046A1 (en) | 1995-09-20 |
NO952194D0 (en) | 1995-06-02 |
AU5655394A (en) | 1994-07-04 |
FI952733A (en) | 1995-06-05 |
NO952194L (en) | 1995-06-02 |
FI952733A0 (en) | 1995-06-05 |
CA2150947A1 (en) | 1994-06-23 |
MX9307679A (en) | 1994-06-30 |
JPH08503955A (en) | 1996-04-30 |
IL107881A0 (en) | 1994-04-12 |
KR950704322A (en) | 1995-11-17 |
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