WO1994008716A1 - Cartouche de dosage - Google Patents

Cartouche de dosage Download PDF

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Publication number
WO1994008716A1
WO1994008716A1 PCT/US1993/009395 US9309395W WO9408716A1 WO 1994008716 A1 WO1994008716 A1 WO 1994008716A1 US 9309395 W US9309395 W US 9309395W WO 9408716 A1 WO9408716 A1 WO 9408716A1
Authority
WO
WIPO (PCT)
Prior art keywords
panel
wells
cartridge
underside
sub
Prior art date
Application number
PCT/US1993/009395
Other languages
English (en)
Inventor
Brent Huntley
Michael Wooding
James Godsey
Original Assignee
Baxter Diagnostics Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Diagnostics Inc. filed Critical Baxter Diagnostics Inc.
Priority to AU52967/93A priority Critical patent/AU5296793A/en
Priority to EP93923202A priority patent/EP0616555A4/fr
Priority to JP6510061A priority patent/JPH07502207A/ja
Publication of WO1994008716A1 publication Critical patent/WO1994008716A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • This invention relates generally to assay cartridges having a ⁇ plurality of aligned adjacent wells which are useful as reaction vessels for performing diagnostic tests in which a solid phase is to be separated from a liquid phase, and is particularly directed to assay cartridges which are adapted so that the liquid phase may be separated from the solid phase by drawing it through a filter o membrane at a lower opening in the wells by means of a pressure differential.
  • Assay cartridges which include a plurality of wells for performing diagnostic assays on samples are known.
  • the present invention s relates to a class of cartridges which are generally described in U.S. Patent No. 4,704,255 to Jolley. These cartridges are designed to perform assays of the type described in U.S. Patent No. 4,652,533, also to Jolley. (For purposes of the present disclosure, both of the above patents are incorporated herein by reference and made a part o hereof.)
  • a sample is inoculated into one or more of the wells in the cartridge.
  • An immunoreactant selected for its ability to bind the analyte of interest is attached to a plurality of water insoluble particles held in a suspended state in reagent liquids. Upon 5 introduction of the suspended particles and liquid into the sample wells, the immunoreactant selectively and/or competitively binds to analyte in the sample.
  • the bottom of each well is covered with a filter membrane designed to retain the suspended particles while permitting liquids to pass through.
  • the particles and any bound o substrate are concentrated by microfiltration, that is, by drawing the liquids by means of a pressure differential through the membrane and into a waste reservoir, which is molded as an integral part of the cartridge.
  • a luminescent labelled material which selectively binds to the immunoreactant and/or the analyte is used to determine whether the analyte is present.
  • an assay cartridge which is relatively simple in design and easy to manufacture. It is a further object of this invention to provide an assay cartridge which can be relatively easily and inexpensively molded, but which utilizes a smaller amount of molded material than existing cartridges.
  • a cartridge for holding an analytical sample during a diagnostic assay comprising a panel which has a plurality of wells in its upper side.
  • the wells have an upper opening and a lower opening; and the lower opening extends to the underside of the panel.
  • Filter means extend across the lower openings of the wells so that liquids contained in the wells may be drawn through the filter, thereby concentrating suspended solids near the lower openings of the wells.
  • the panel includes a support means for supporting the panel on a non-integral, external surface, which does not form a part of the panel.
  • the underside of the panel, the support means, and the surface When placed on a surface, the underside of the panel, the support means, and the surface thereby define a substantially enclosed sub-panel region.
  • a negative pressure differential is created in the sub- panel region, air is drawn through the wells from the upper side of the panel, thereby drawing liquid from the wells into the sub-panel region.
  • the surface supporting the panel is part of an automated or semi-automated instrument designed to perform the various steps of a diagnostic assay sequentially on an amplified or 5 non-amplified nucleic acid target.
  • the panel is removably attached to the support surface and the support surface can be moved to various positions within the instrument for addition of reagents, removal of liquids from the wells, and the like.
  • FIG. 1 is an exploded perspective view of an assay cartridge and s associated apparatus made in accordance with the present invention
  • FIG. 2 is a cross-sectional view of the assay cartridge and associated apparatus in their assembled configuration
  • FIG. 3 is a top view of the assay cartridge and associated apparatus in its assembled configuration, including the cartridge o locating feature;
  • FIG. 4 is a cutaway view of an instrument which may be used to perform assays with the cartridge of the present invention.
  • FIG. 5 is a perspective view of the front of an instrument which may be used to perform assays with the cartridge of the present 5 invention, showing the cartridge just after it has been loaded into the instrument.
  • Figs. 1 and 2 show the general arrangement of an assay cartridge 10 made in accordance with the present invention.
  • This o Specification describes a preferred form of the invention, in which the assay cartridge is used in conjunction with an automated or semi- automated instrument for performing diagnostic assays and, in particular, probe-based assays.
  • movement of the cartridge, the addition of reagents to the appropriate wells in the cartridge, as well as the various washing steps, are performed robotically by the instrument.
  • the cartridge and associated apparatus of the present invention may also be used to perform such assays manually.
  • the invention in general includes an assay cartridge in the form of a panel 12, together with associated equipment for drawing liquid reagents through lower openings in the bottoms of the wells 14 in the cartridge.
  • various liquid reagents and washing solutions are added to the wells 14 in the cartridge 12. Excess reagents and washing solutions can be easily and efficiently removed from the wells using the cartridge and associated apparatus described herein.
  • This apparatus includes a vacuum plate 16 with a vacuum well 17 formed therein and a rubber gasket 18 adapted to maintain an airtight seal between the cartridge 12 and the vacuum plate 16.
  • the vacuum plate 16 is secured to a base plate 19.
  • the base plate 19 has a channel 20 and a vacuum orifice 22 formed therein.
  • the vacuum orifice 22 is aligned with a corresponding opening 24 in the vacuum plate 16.
  • a negative pressure differential is also created in the vacuum well 17, thereby causing fluids in the cartridge wells 14 to be drawn by means of the pressure differential into the vacuum well and through the channel 20.
  • the cartridge is preferably inexpensively manufactured so that it can be disposed of after use.
  • the assay cartridge of the present invention is a plate or panel 12 having an upper side, an underside, and a plurality of wells 14 formed therein.
  • the cartridge illustrated takes the general form of a conventional generally rectangular microtitre plate and preferably has either 96 (8 rows of 12) or 48 (8 rows of 6) wells.
  • Each well 14 has an upper opening
  • each well 14 takes the form of a generally cylindrical recess formed in the cartridge 12, and tapers at its lower end to a lower opening 26.
  • the wells can be generally parabolic or conical in shape, but truncated at their lower o ends to form the lower openings.
  • Each lower opening 26, in turn, is covered with a porous microfilter membrane 28, which is designed to filter and retain suspended solids while permitting unwanted liquids and reagents to pass through.
  • a porous microfilter membrane 28 which is designed to filter and retain suspended solids while permitting unwanted liquids and reagents to pass through.
  • the size, shape, s configuration, and method of manufacture of the wells, as well as that of the microfilter may be as described in U.S. Patent No. 4,704,255.
  • support means in the form of a supporting wall 30 is formed adjacent the periphery of the cartridge o on the underside thereof and extends around the perimeter of the cartridge.
  • This supporting wall 30 has a height greater than the depth of the wells 14 (including the depth of the filter membrane 28) so that the when the cartridge is placed on a flat surface, the lower openings of the wells are elevated above the level of that surface.
  • the supporting wall 30 also has a substantially flat lower surface, which is adapted to engage a flat surface at substantially all points along the length of the wall.
  • the supporting wall 30 is illustrated as a generally rectangular, continuous wall which extends substantially normally from the underside of the o cartridge at a position adjacent the peripheral edge of the cartridge.
  • the wall 30 also has a substantially flat lower surface adapted to sealingly engage the flat gasket 18.
  • the wall may be formed in any number of ways, as long as the sub-panel region 50 is substantially enclosed so that a negative pressure may be created therein in order to withdraw fluids from the wells.
  • the cartridge is generally open in the sub-panel region 50 5 (see Fig. 2) beneath the lower openings of the wells.
  • the region is referred to herein as a "sub-panel” region, it will be understood that it need not be located below the panel if, for example, the panel is not supported on a horizontal surface. Thus, this term is meant to encompass any possible orientation of the o support surface.
  • the sub-panel region 50 beneath the lower openings of the wells and interior of the support wall 30 is substantially uncovered at the lower side thereof.
  • the cartridge may be placed on a generally horizontal surface in order to substantially enclose the sub-panel region such that significant s quantities of air may only enter the sub-panel region from the upper side of the panel and through the upper openings of the wells.
  • means are then provided for creating a negative pressure in this enclosed sub-panel region to withdraw fluids from the wells, through the filter membrane, and into the sub-panel region. o It will also be understood that it may be desirable in certain circumstances to partially cover the bottom of the panel. For example, after liquid from the wells is withdrawn, remnants of the fluid frequently adhere to the underside of the panel.
  • the 5 bottom of the panel be covered in some manner, for example, by a flexible sheet made of Mylar ® or another suitable material, and affixed to the lower surface of the supporting wall.
  • this covering typically will be partially punctured in some manner when the cartridge is place on the vacuum plate so that a negative pressure o may be created in the sub-panel region; however, such a covering will help minimize exposure to any remaining fluid adhering to the underside of the panel.
  • the panel 12 has a pair of integral ledges 32, 33 (only ledge 32 is visible in Fig. 1 ) which extend outwardly from the supporting wall 30. If the panel 12 is generally rectangular, as is illustrated in Figs. 1 and 2, ledges 32, 34 are preferably located on the shorter sides of the
  • an outside surface of the supporting wall also preferably includes a generally smooth area (which may also be recessed) for receiving an adhesive-backed bar code label 36.
  • the label 36 is used to identify the panel itself or, if the cartridge is being used in a clinical setting, the patient from whom the specimens being tested in the s cartridge were taken.
  • the cartridge may be manufactured by injection molding it in the manner described in U.S. Patent No. 4,704,255.
  • the cartridge of the present invention is unlike that disclosed in the '255 patent, however, in that it lacks, among other things, an intermediate section and an o integral waste reservoir.
  • the cartridge may be molded as a single, integral piece, thereby greatly reducing the complexity and cost of the mold, as well as the amount of molded material required.
  • the material selected should be able to withstand the 5 various temperature cycles encountered during modern diagnostic procedures.
  • the cartridge must also be sufficiently rigid to withstand significant warpage when the wells are evacuated under negative pressure, as will be described in greater detail below.
  • Presently preferred materials which may be used to mold the cartridge include o polystyrene, polysulfone, polycarbonate, ABS, polytetrafluoroethylene (PTFE), and polypropylene.
  • PTFE polytetrafluoroethylene
  • the cartridge may be manufactured out of other materials (including metals) and using other manufacturing methods (such as forming, stamping, and the like).
  • Filter means in the form of a filter member 28 may be formed as a sheet and positioned so that it extends across and against the lower 5 openings of the wells in any of the ways described in U.S. Patent No. 4,704,255.
  • Preferred filter materials include cellulose acetate, polypropylene, PTFE, and polycarbonate. To date, success has been had with a filter membrane made of cellulose acetate manufactured by Sartorius of Bohemia, Long Island, New York.
  • a sheet-like filter membrane is joined or attached to the lower edges of the wells which surround the lower openings 26 of the wells 14 by insert molding such that these openings are completely covered by the membrane and liquid cannot leak around the membrane, but must pass through the membrane when it is forcibly evacuated from the s wells.
  • filter membranes having a unique and novel composition may be used to manufacture the cartridges of the present invention, particularly when the cartridge is to be used for probe-based assays.
  • These membranes are comprised o of both hydrophilic and hydrophobic materials which have been assembled together in various ways to form an integral membrane.
  • the membrane may be comprised of hydrophilic and hydrophobic layers of filter materials which have been welded together to form a "sandwich.”
  • the membrane may be a 5 single layer having alternating "strips" of hydrophilic and hydrophobic filter materials.
  • the membrane could be comprised of either a hydrophilic or a hydrophobic material with inserts (for example, circular inserts) made of a hydrophobic or hydrophilic material, respectively. These inserts could be dispersed o substantially uniformly throughout the membrane.
  • the support surface is not attached to, or a part of, the cartridge but, in cooperation with the cartridge, defines the enveloped sub-panel region.
  • this support surface will be part of a separate instrument in which the panel is used.
  • the lower surface of the supporting wall 30 of the panel o 12 sealingly engages a gasket 18 resting on a vacuum plate 16 so that unwanted liquids may be removed from the wells 14 by negative air pressure in the sub-panel region.
  • sealing means in the form of a gasket 18 is interposed between the cartridge 10 and the vacuum plate 16, and downward pressure is exerted on 5 the cartridge 10.
  • the gasket 18 is substantially rectangular, open in the area positioned beneath the wells 14, and sufficiently wide so that the bottom surface of the supporting wall 30 can be brought into complete engagement with the gasket at substantially all points along its length.
  • the o gasket is made of rubber or another compressible material, such as well known polymers or monomers, so that it will provide a substantially airtight seal between the cartridge 12 and the vacuum plate 16 as will now be described.
  • the gasket is affixed to the surface of the vacuum plate 16 by means of a suitable adhesive.
  • the gasket could be molded (e.g., insert molded) as part of the vacuum plate (if the 5 vacuum plate is molded), or as a separate piece.
  • the gasket could be inserted into an appropriately shaped depression in the vacuum plate in an interference fit relationship, or stretched around a lip on the surface of the vacuum plate.
  • the sealing means is illustrated as a gasket 18 in Fig. 1 , it will be understood that it may s also take numerous other configurations, such as an O-ring affixed to the inner walls of the well 17 around its periphery, which is adapted to sealably engage the outer surface of the supporting wall 30; a groove around the periphery of the well 17, which is adapted to sealably accept the supporting wall 30 in sealing engagement; a o sealing projection, such as a flexible O-ring, on the outer edges of the cartridge itself; or other like configurations.
  • the vacuum plate 16 is preferably formed of a suitable rigid material, such as aluminum or reinforced plastic, and preferably includes a vacuum well 17 formed in the surface of the plate which 5 faces the underside of the panel 12.
  • the plate may be formed of any suitable material, as long as it is sufficiently durable to withstand repeated use.
  • the vacuum well 17 is illustrated as a generally wedge-shaped depression in the vacuum plate 16, which is formed by machining.
  • the well may take any number of shapes and may be formed by casting, molding, and other well known methods.
  • An opening 24 through the vacuum well 17 is formed in the vacuum plate 24 by suitable methods, such as drilling, forming, machining, casting, or the like.
  • the opening 24 is located in the deepest area of the well 17, so that gravity will assist in funnelling the liquids to the opening where they are withdrawn under pressure from the well 17.
  • This opening is used to create a negative pressure differential in the sub-panel region, as will be described 5 below.
  • this opening could also be located in the panel itself as long as it communicates with the sub- panel region.
  • a sponge or another absorbent or adsorbent material may be placed in the vacuum well 17. o
  • the use of such materials assists in efficiently withdrawing fluids from the wells 14 and in preventing an accumulation of fluid on the underside of the cartridge, which is typically undesirable.
  • the well may be formed with a textured interior surface or an integral or non-integral manifold in proximity with the panel s which is similarly designed to prevent the accumulation of liquids by channelling them away from the panel.
  • the vacuum plate 16 in turn, is rigidly mounted to base plate 19 by suitable means, such as screws 40 threaded into corresponding threaded recesses 42 in the base plate 19.
  • the base plate may be o formed from the same materials and in the same manner as the vacuum plate. Typically, however, the base plate will be formed of a suitable metal, such as aluminum or reinforced plastic.
  • the base plate 19 forms part of an associated automated or semi-automated instrument, 5 which includes apparatus for moving the plate to various stations for addition of reagents to the wells, incubation, washing, and other processes commonly employed in diagnostic procedures.
  • the vacuum plate 16 be removably attached to the base plate 19 rather than integrally formed therewith, although o the latter is also an acceptable configuration.
  • a removable vacuum plate is preferred so that the vacuum plate 19 may be replaced with another plate sized to accommodate cartridges of varying sizes or shapes. This provides a configuration with a maximum amount of flexibility to accommodate various currently available cartridges and cartridges which may be developed in the future.
  • a channel 20 and vacuum orifice 22 is formed in the base plate
  • the vacuum orifice 22 is positioned so that it is brought into substantial alignment or registry with the opening 24 in the vacuum plate 16 when the vacuum plate is rigidly attached to the base plate 19.
  • a sealing means such as an O- ring 44 inserted into a circular groove (not shown) in the base plate 19, is positioned between the vacuum plate 16 and the base plate 19 in the area surrounding the aligned opening 24 and vacuum orifice 22 to substantially prevent leakage of air due to positional tolerances and the like.
  • the channel 20, which communicates with the vacuum orifice 22, is formed in the base plate 19.
  • a negative pressure can be created in the channel 20 and, via the vacuum orifice
  • the cartridge 12 is assembled to the vacuum plate 16 in the following manner. Referring simultaneously to Figs. 3 and 4, integral ledge 33 on the panel 12 is positioned under the latch 38.
  • the latch 38 is shaped and positioned to engage the ledge 33 at the adjacent end of the panel 12. (Ledge 33 is similar to the ledge 32 at the other end of the panel 12, except that no grooves 34 need be provided.)
  • the panel 12 is then placed between lateral positioning guides, which may take the form of a pair of stanchions 46a, 46b rigidly attached to the vacuum plate 16 adjacent the long edges of the panel 12 and substantially intermediate their length.
  • the stanchions 46a, 46b help to guide the panel 12 into position so that the V-shaped grooves 34 in the ledge 34 are brought into accepting engagement with the locating guides 48a, 48b, which are rigidly attached to the vacuum plate 16 adjacent the shorter edge of the cartridge 12 opposite the latch 38.
  • the cartridge is precisely located with respect to the vacuum plate 16 and the base plate 19.
  • the plate be located with sufficient precision so that the centers of the wells may be located to within .001 inch and, preferably, about .005 inch. This is particularly important when the cartridge is used with an automated or semi- automated instrument, since in order to move the cartridge to 5 appropriate stations so that the appropriate reagents and wash solutions may be dispensed into the correct wells in the cartridge, the location of the cartridge (and, thus, the location of the wells) must be fixed so that it can be known within fairly precise limits.
  • the latch 38 is preferably slidably o attached to the base plate 19 so that it can be moved in a downward direction.
  • the latch 38 can be mechanically urged (for example, pneumatically or by means of a solenoid) toward the vacuum plate 16 in order to press the lower surface of the supporting wall 30 into an interference fit relationship with the gasket 18, s thereby compressing the gasket and forming a substantially airtight seal.
  • a similar latch (not shown), either movable or fixed, may be provided at the opposite end of the cartridge to engage the opposite ledge 32 on the cartridge (see Fig. 1 ) and thereby uniformly hold the other side of the cartridge against the gasket 18.
  • the pump P should be operated at pressures which efficiently and substantially remove liquids from the panel wells 14 in order to effectively concentrate the solids remaining in the wells near the bottom openings of the wells. While such pressures may be readily selected and optimized by those skilled in o the art depending on the configuration of the entire system, in many applications such pressures will be approximately 22 inches of mercury or greater.
  • Figs. 4 and 5 illustrate schematically an instrument which may be used to mechanically manipulate the cartridge in order to move the s wells to and from various stations for dispensing reagents and the like during the course of performing a diagnostic assay.
  • the instrument 60 includes means for moving the base plate 19, together with the attached vacuum plate 16 and panel 12, to substantially all locations within a plane which is parallel to the planes o of the base plate 19, vacuum plate 16 and panel 12, but perpendicular to the face of the instrument.
  • motors 64a, 64b are rigidly attached to the base plate 19 and powered by motors 64a, 64b.
  • motors 64a, 64b may be stepper motors, servo 5 motors, or the like.
  • the motors 64a, 64b are under the control of a central processor (not shown) which, in response to input from the instrument operator, as well as other internal inputs, actuates the motors to power the lead screws 62a, 62b and thereby position the cartridge in the desired location within the instrument.
  • lead screw 62a is adapted to move the base plate 19 in a first or "X" direction, while lead screw 62b moves the base plate 19 in a second or “Y” direction substantially perpendicular thereto. It will be seen that by sequential actuation of the motors 64a, 64b, base plate 19 may be moved to precisely defined Cartesian coordinates within the plane of motion, thereby positioning the panel 12 and its wells 14 in desired locations within the instrument so that reagents may be dispensed into selected wells at appropriate times during the course of a diagnostic assay.
  • Fig. 5 illustrates the position of the cartridge just after it is loaded into the instrument 60 or just prior to the time it is removed from the instrument.
  • motors 64a, 64b By actuating motors 64a, 64b, the base plate 19 and attached vacuum plate 16 are brought into alignment with slot 66 in the front face of the instrument 60. Motor 64b is then actuated o to move the base plate 19 through the slot such that the cartridge is brought outside the body of the instrument.
  • the cartridge may be manually inserted into, or removed from its fixed position on the vacuum plate 16.
  • the cartridge is inserted prior to the start of the assay and removed after the assay is 5 completed, although it will be understood that it may be desirable to remove the cartridge during the course of the assay for addition of reagents and the like.
  • motors 64a, 64b are o actuated to position the base plate 19 and attached vacuum plate 16 in the general position shown in Fig. 5.
  • the operator then attaches a panel 12 to the vacuum plate 16 in sealing engagement with the gasket in the proper position, being guided by the locating guides on the vacuum plate 16, as described above.
  • one or more wells 14 Prior to attachment of the 5 panel 12, one or more wells 14 are inoculated with an appropriate sample -- for example, a sample of a bodily fluid which has or has not been subjected to nucleic acid amplification.
  • Motors 64a, 64b are then actuated to bring the cartridge into the body of the instrument 60 and to move the appropriate well(s) into o alignment with various dispensing or incubating stations, where appropriate detection and washing reagents are dispensed into the wells.
  • pump P (see Fig. 2) is actuated in order to create a negative pressure in the sub-panel region beneath the wells 14. This negative pressure differential uniformly draws fluids in the wells 14 into the vacuum well 17 and out through the opening 24 and the vacuum orifice 22. At this point, the fluids are drawn through the channel 20 in the base plate 19 and, preferably, into a waste container (not shown) for safe disposal.
  • the solids which have been concentrated on the membrane at the lower ends of the wells 14 can then be analyzed through well-known detection methods, such as fluorometry, spectrophotometry, radiometry, and the like. (See, for example, the detection methods described in U.S. Patent No. 4,652,533.) While the invention has been described in connection with certain presently preferred components and arrangements, those skilled in the art will recognize many modifications to structure, arrangement, portions, elements, materials, steps, and components which can be used in the practice of the invention without departing from the principles thereof.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Une cartouche destinée à recevoir un échantillon d'analyse pendant un dosage diagnostique comprend un panneau comportant dans sa face supérieure une pluralité d'alvéoles juxtaposées. Lesdites alvéoles comportent des ouvertures supérieure et inférieure, l'ouverture inférieure s'étendant vers la face inférieure du panneau. Une membrane à filtre est disposée en travers des ouvertures inférieures des alvéoles de manière que les liquides contenues dans les alvéoles soient aspirés à travers le filtre, ce qui a pour résultat d'accumuler les solides en suspension près des ouvertures inférieures des alvéoles. Le panneau comprend une cloison permettant le maintien du panneau sur une surface séparée externe qui ne fait pas partie du panneau.
PCT/US1993/009395 1992-10-09 1993-10-04 Cartouche de dosage WO1994008716A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU52967/93A AU5296793A (en) 1992-10-09 1993-10-04 Assay cartridge
EP93923202A EP0616555A4 (fr) 1992-10-09 1993-10-04 Cartouche de dosage.
JP6510061A JPH07502207A (ja) 1992-10-09 1993-10-04 アッセイカートリッジ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95920792A 1992-10-09 1992-10-09
US07/959,207 1992-10-09

Publications (1)

Publication Number Publication Date
WO1994008716A1 true WO1994008716A1 (fr) 1994-04-28

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ID=25501777

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/009395 WO1994008716A1 (fr) 1992-10-09 1993-10-04 Cartouche de dosage

Country Status (5)

Country Link
EP (1) EP0616555A4 (fr)
JP (1) JPH07502207A (fr)
AU (1) AU5296793A (fr)
CA (1) CA2125159A1 (fr)
WO (1) WO1994008716A1 (fr)

Cited By (7)

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Publication number Priority date Publication date Assignee Title
WO1998053912A1 (fr) * 1997-05-27 1998-12-03 Qiagen Gmbh Dispositif de filtration, sous pression reduite et de maniere selective, et de sechage sous vide d'echantillons liquides ou de gouttes d'echantillons liquides et utilisation de ce dispositif
WO1999047928A2 (fr) * 1998-03-19 1999-09-23 Immunetics, Inc. Systemes et procedes de criblage rapide par buvardage
GB2348622A (en) * 1999-03-16 2000-10-11 Minerva Technologies Moulded filter plates
GB2377990A (en) * 2001-07-17 2003-01-29 Vivascience Ltd Multiwell plate
US20030039592A1 (en) * 1997-03-25 2003-02-27 Greiner Bio-One Gmbh Microplate with transparent bottom
CH693689A5 (de) * 2002-05-31 2003-12-15 Tecan Trading Ag Vorrichtung, System und Verfahren zum Übertragen von Flüssigkeiten aus SPE-Platten.
EP1591164A1 (fr) * 2004-04-23 2005-11-02 Millipore Corporation Plaque à cavités multiples avec volume de rétention réduit

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104155204A (zh) * 2014-08-15 2014-11-19 广州衡创测试技术服务有限公司 一种水中悬浮物过滤方法

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Also Published As

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CA2125159A1 (fr) 1994-04-28
AU5296793A (en) 1994-05-09
EP0616555A4 (fr) 1996-08-28
EP0616555A1 (fr) 1994-09-28
JPH07502207A (ja) 1995-03-09

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