WO1994002819B1 - Apparatus and method for testing condoms as barriers to virus penetration - Google Patents
Apparatus and method for testing condoms as barriers to virus penetrationInfo
- Publication number
- WO1994002819B1 WO1994002819B1 PCT/US1993/006705 US9306705W WO9402819B1 WO 1994002819 B1 WO1994002819 B1 WO 1994002819B1 US 9306705 W US9306705 W US 9306705W WO 9402819 B1 WO9402819 B1 WO 9402819B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- condom
- conduit
- pressurizing
- container
- viral particles
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title 1
- 230000035515 penetration Effects 0.000 title 1
- 230000003612 virological Effects 0.000 claims abstract 20
- 239000002245 particle Substances 0.000 claims abstract 19
- 230000000452 restraining Effects 0.000 claims abstract 15
- 239000007788 liquid Substances 0.000 claims abstract 14
- 239000012530 fluid Substances 0.000 claims abstract 10
- 210000003899 Penis Anatomy 0.000 claims abstract 5
- 230000000875 corresponding Effects 0.000 claims abstract 5
- 239000000725 suspension Substances 0.000 claims abstract 5
- 230000008478 viral entry into host cell Effects 0.000 claims abstract 3
- 238000011049 filling Methods 0.000 claims abstract 2
- 239000004033 plastic Substances 0.000 claims 7
- 229920003023 plastic Polymers 0.000 claims 7
- 239000004744 fabric Substances 0.000 claims 6
- 239000011521 glass Substances 0.000 claims 6
- 239000000843 powder Substances 0.000 claims 6
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 claims 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 4
- 239000000919 ceramic Substances 0.000 claims 3
- 230000002706 hydrostatic Effects 0.000 claims 3
- 239000000463 material Substances 0.000 claims 3
- 229920000515 polycarbonate Polymers 0.000 claims 3
- 239000004417 polycarbonate Substances 0.000 claims 3
- 229920000379 polypropylene carbonate Polymers 0.000 claims 3
- 241001515965 unidentified phage Species 0.000 claims 3
- 238000002156 mixing Methods 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- 239000004677 Nylon Substances 0.000 claims 1
- 108060007474 POR Proteins 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 239000000314 lubricant Substances 0.000 claims 1
- 238000003760 magnetic stirring Methods 0.000 claims 1
- 229920001778 nylon Polymers 0.000 claims 1
- 239000006223 plastic coating Substances 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000000837 restrainer Substances 0.000 abstract 2
- 230000001568 sexual Effects 0.000 abstract 1
Abstract
A method for testing a condom for viral penetration includes the steps of positioning a condom to be tested in a restraining means so that expansion of the condom is restricted to a size corresponding to the mean anthropomorphic dimensions of a penis; pressurizing the condom via a liquid containing viral particles in suspension, and monitoring for passage of viral particles through the condom. The method which simulates conditions of human sexual intercourse while using a viral particle to test condom integrity can be effected using an apparatus with elements that are commonly found in a microbiology lab. The apparatus includes a conduit over which the open end of the condom to be tested is secured; a restrainer for restricting the expansion of the condom to a size corresponding to the mean anthropomorphic dimensions of a penis; a liquid containing viral particles in suspension, the liquid filling the condom; a pressurizing device for pressurizing the condom via the liquid; and a container filled with a collecting fluid wherein the condom and the restrainer are positioned so that the viral particles that have passed through the condom can be monitored.
Claims
_ 1g _
AMENDED CLAIMS
[received by the International Bureau on 25 January 1994 (25.01.94); original claims 8, 9, 18, 19, 21, 23 and 32-34 amended; new claims 35-40 added; remaining claims unchanged (6 pages)]
6. The apparatus of claim 1, further comprising means for circulating said collection fluid.
7. The apparatus of claim 1, wherein said conduit comprises a Buchner funnel .
8. The apparatus of claim 1, wherein said means for securing comprises at least one rubber band.
9. The apparatus of claim 1, wherein said restraining means comprises an open weave fabric.
10. The apparatus of claim 9, wherein said open fabric comprises organdy fabric.
11. The apparatus of claim 9, wherein said open fabric comprises nylon fabric.
12. The apparatus of claim 2, wherein said second means for securing comprises at least one rubber band.
13. The apparatus of claim 3, wherein said leakage indicator comprises a piece of pH paper.
14. The apparatus of claim 4, wherein said means for protecting is parafilm, wax paper, or plastic kitchen wrap.
15. The apparatus of claim 1, wherein said liquid containing said viral particles comprises a sterile Dulbecco's phosphate buffered saline.
16. The apparatus of claim 1, wherein said viral particles comprise a 0X174 bacteriophage.
17. The apparatus of claim 1, wherein said viral particles are a PRD1, MS2, T7, or 06 bacteriophage.
18. The apparatus of claim 1, wherein said conduit includes a second end, said means for pressurizing comprises a piece of tubing and a funnel, and wherein one end of said tubing is connected to said second end of said conduit and the other end of said tubing is connected to said funnel.
19. The apparatus of claim 1, wherein said means for pressurizing includes means for pressurizing said liquid containing viral particles in suspension to about 60 mm Hg.
20. The apparatus of claim 1, wherein said container comprises a fleaker having its top restriction removed.
21.- The apparatus of claim 5, wherein said means for positioning comprises a clamp and a ringstand.
22. The apparatus of claim 1, wherein said collection fluid comprises a sterile Dulbecco's phosphate buffered saline.
23. The apparatus of claim 6, wherein said means for circulating comprises a magnetic stirring bar having a sterilizable plastic coating.
24. An apparatus for testing viral penetration through a condom having an open end, comprising:
(A) a conduit having a first end, said open end of said condom being placed over said first end of said conduit;
(B) means for securing said open end of said condom over said first end;
(C) restraining means for restricting hydrostatic expansion of said condom to a size corresponding to mean anthropomorphic dimensions of a penis, said restraining means having an open end and being virally penetrable and virally inert, wherein said restraining means is positioned relative to said condom such that said restraining means envelopes a portion of said condom ;
(D) a liquid containing viral particles in suspension, said liquid filling said condom;
(E) means for pressurizing said liquid;
(F) a container capable of holding liquid, wherein said condom and said restraining means are positioned in said container; and
(G) a collection fluid in said container for collecting said viral particles that have passed through said condom.
25. A method for testing a condom for viral penetration, comprising the steps of : positioning a condom in a restraining means for restricting hydrostatic expansion of said condom to a size corresponding to mean anthropomorphic dimensions of a penis; pressurizing said condom by introducing thereinto a liquid that contains viral particles in suspension; and monitoring for passage of any viral particles through said condom.
26. The method of claim 25, wherein the positioning step comprises the step of positioning said condom and said restraining means in a container filled with a collection fluid.
27. The method of claim 26, wherein the monitoring step comprises the step of monitoring for any viral particles at about 30 minutes after said pressurizing step.
28. The method of claim 25, further comprising the step of rinsing lubricant from said condom and drying said condom before performing the positioning step.
29. The method of claim 25, wherein the pressurizing step comprises the step of pressurizing said condom to a pressure of about 60 mm Hg.
30. The method of claim 26, further comprising the step of assaying said collection fluid before performing the positioning step to assure that none of said viral particles are present in said collection fluid.
31. The method of claim 27, wherein the monitoring step includes the steps of stirring said collection fluid and then assaying said collection fluid.
32. An apparatus in the form of a kit for testing a condom having an open end for viral penetrability, comprising:
(A) a conduit having first and second ends, said first end of said conduit for receiving said open end of said condom;
(B) means for securing said open end of said condom over said first end;
(C) restraining means for restricting hydrostatic expansion of said condom to a size corresponding to mean anthropomorphic dimensions of a penis, said restraining means having an open end and being virally penetrable and virally inert;
(D) at least one viral particle;
(E) a first dried buffer powder for mixing with water and for suspending therein said at least one viral particle to form a liquid;
(F) a container capable of holding liquid, wherein said condom and said restraining means are to be positioned in said container; and
(G) a second dried buffer powder for mixing with water to form a collection fluid for collecting said viral particles that have passed through said condom.
33. The apparatus of claim 32, further comprising: second means for securing said open end of said restraining means over said first end of said conduit; a leakage indicator to be placed between said first end of said conduit and said open end of said condom nearer to said open end of said condom than said securing means; means for protecting said open end of said condom, said protecting means to be placed between said open end of said condom and said means for securing; means for positioning said conduit so that said condom and said restraining means will be positioned in said container; and means for pressurizing said liquid, wherein said pressurizing means is to be connected to said second end of said conduit.
34. The apparatus of claim 33, wherein said conduit comprises a Buchner funnel; said means for securing comprises at least one rubber band; said restraining means for restricting comprises an open weave fabric; said second means for securing comprises at least one rubber band; said leakage indicator comprises a piece of pH paper; said means for protecting is parafilm, wax paper, or plastic kitchen wrap; said first dried buffer powder comprises a sterile Dulbecco's phosphate buffered saline powder; said at least one viral particle is a X174, PRDl, MS2, T7, or φ6 bacteriophage; said means for pressurizing comprises a piece of tubing and a funnel, one end of said tubing is to be connected to said second end of said conduit and the other end of said tubing is to be connected to said funnel; said container comprises a fleaker having its top restriction removed; said means for positioning comprises a clamp and a ringstand; and said second dried buffer powder comprises a sterile Dulbecco's phosphate buffered saline powder.
35. The apparatus of claim 1, wherein the conduit, the means for pressurizing, and the container are made from a material which is heat sterilizable.
36. The apparatus of claim 35, wherein the conduit is made from one of glass, ceramic or plastic; the means for pressurizing is made from plastic; and the container is made from one of glass, polypropylene, or polycarbonate.
37. The apparatus of claim 24, wherein the conduit, the means for pressurizing, and the container are made from a material which is heat sterilizable.
38. The apparatus of claim 37, wherein the conduit is made from one of glass, ceramic or plastic; the means for pressurizing is made from plastic; and the container is made from one of glass, polypropylene, or polycarbonate.
39. The apparatus of claim 32, wherein the conduit and the container are made from a material which is heat sterilizeable.
40. The apparatus of claim 39, wherein the conduit is made from one of glass, ceramic or plastic and wherein the container is made from one of glass, polypropylene, or polycarbonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU47748/93A AU4774893A (en) | 1992-07-20 | 1993-07-20 | Apparatus and method for testing condoms as barriers to virus penetration |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/915,581 | 1992-07-20 | ||
| US07/915,581 US5422240A (en) | 1992-07-20 | 1992-07-20 | Apparatus and method for testing condoms as barriers to virus penetration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1994002819A1 WO1994002819A1 (en) | 1994-02-03 |
| WO1994002819B1 true WO1994002819B1 (en) | 1994-03-17 |
Family
ID=25435961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1993/006705 WO1994002819A1 (en) | 1992-07-20 | 1993-07-20 | Apparatus and method for testing condoms as barriers to virus penetration |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US5422240A (en) |
| AU (1) | AU4774893A (en) |
| WO (1) | WO1994002819A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5493899A (en) * | 1994-05-23 | 1996-02-27 | Donald Guthrie Foundation For Education And Research | Method for testing integrity of elastomeric protective barriers |
| US5681742A (en) * | 1995-09-26 | 1997-10-28 | Louisville Laboratories, Inc. | Biological specimen containment and incubation device |
| US5659130A (en) * | 1996-04-03 | 1997-08-19 | Industrual Technology Research Institute | Simple device for testing chemical permeation |
| US6164122A (en) * | 1999-04-21 | 2000-12-26 | Carter-Wallace, Inc. | System and method including multiple test chambers for automated testing for holes in prophylactic device |
| EP1254250B1 (en) | 2000-01-26 | 2005-03-23 | Loma Linda University | Method for the evaluation of implantable materials |
| US6430989B1 (en) * | 2000-12-29 | 2002-08-13 | Kimberly-Clark Worldwide, Inc. | Method and apparatus for evaluating swimwear for underwater leakage |
| US20050189368A1 (en) * | 2003-12-04 | 2005-09-01 | Osterberg Brian J. | Combination beverage service item and condom holder |
| TWM318124U (en) * | 2007-01-05 | 2007-09-01 | Smart Ant Telecom Co Ltd | Examining apparatus for dust-proof and waterproof capabilities |
| CN104792471A (en) * | 2015-04-03 | 2015-07-22 | 天津市计划生育药具管理中心 | Female condom pinhole detecting method |
| DE102015005833A1 (en) * | 2015-05-07 | 2016-11-10 | Sartorius Stedim Biotech Gmbh | Method and device for an integrity test of a test container |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1295249A2 (en) * | 1985-09-02 | 1987-03-07 | Войсковая часть 13991 | Method of checking leak-proofness of articles |
| US4843014A (en) * | 1987-12-02 | 1989-06-27 | Cukier Daniel S | Apparel having a breach indicator |
| US5073482A (en) * | 1988-06-21 | 1991-12-17 | Epitope, Inc. | Elastic barrier permeability testing device and method of use |
| JP2765881B2 (en) * | 1988-11-09 | 1998-06-18 | 三菱電機株式会社 | Intake air amount measurement device for internal combustion engine |
| US5138871A (en) * | 1990-04-06 | 1992-08-18 | The United States Of America As Represented By The Department Of Health And Human Services | Method and apparatus for testing the permeability of prophylactics |
-
1992
- 1992-07-20 US US07/915,581 patent/US5422240A/en not_active Expired - Lifetime
-
1993
- 1993-07-20 AU AU47748/93A patent/AU4774893A/en not_active Abandoned
- 1993-07-20 WO PCT/US1993/006705 patent/WO1994002819A1/en active Application Filing
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