WO1993018163A3 - Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria - Google Patents

Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria Download PDF

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Publication number
WO1993018163A3
WO1993018163A3 PCT/US1993/002355 US9302355W WO9318163A3 WO 1993018163 A3 WO1993018163 A3 WO 1993018163A3 US 9302355 W US9302355 W US 9302355W WO 9318163 A3 WO9318163 A3 WO 9318163A3
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WO
WIPO (PCT)
Prior art keywords
delivery
expression
positive bacteria
gram positive
hybrid
Prior art date
Application number
PCT/US1993/002355
Other languages
French (fr)
Other versions
WO1993018163A2 (en
Inventor
Vincent A Fischetti
Gianni Pozzi
Olaf Schneewind
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Priority to AU39199/93A priority Critical patent/AU674311B2/en
Priority to EP93908349A priority patent/EP0646176B1/en
Priority to HU9402575A priority patent/HU220420B/en
Priority to JP51605193A priority patent/JP3626181B2/en
Priority to DK93908349T priority patent/DK0646176T3/en
Priority to AT93908349T priority patent/ATE269904T1/en
Priority to DE69333560T priority patent/DE69333560T2/en
Priority to US08/302,756 priority patent/US6737521B1/en
Publication of WO1993018163A2 publication Critical patent/WO1993018163A2/en
Publication of WO1993018163A3 publication Critical patent/WO1993018163A3/en

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/385—Haptens or antigens, bound to carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6031—Proteins
    • A61K2039/6068—Other bacterial proteins, e.g. OMP
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Process is described for the delivery and expression of hybrid surface proteins to the surface of bacteria. The transformed bacteria are useful as vaccines, for the delivery of other active peptides to animal hosts, as diagnostic reagents and for other purposes.
PCT/US1993/002355 1990-05-11 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria WO1993018163A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU39199/93A AU674311B2 (en) 1992-03-13 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria
EP93908349A EP0646176B1 (en) 1992-03-13 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria
HU9402575A HU220420B (en) 1992-03-13 1993-03-12 A hybrid surface protein of gram positive bacteria, method and kit for detecting of infection
JP51605193A JP3626181B2 (en) 1992-03-13 1993-03-12 Supply and expression of hybrid surface proteins on the surface of Gram-positive bacteria
DK93908349T DK0646176T3 (en) 1992-03-13 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram-positive bacteria
AT93908349T ATE269904T1 (en) 1992-03-13 1993-03-12 TRANSPORT AND EXPRESSION OF A HYBRID SURFACE PROTEIN ON THE SURFACE OF GRAMP POSITIVE BACTERIA
DE69333560T DE69333560T2 (en) 1992-03-13 1993-03-12 TRANSPORT AND EXPRESSION OF A HYBRID SURFACE PROTEIN ON THE SURFACE OF GRAMPOSITIVE BACTERIA
US08/302,756 US6737521B1 (en) 1990-05-11 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85108292A 1992-03-13 1992-03-13
US07/851,082 1992-03-13

Publications (2)

Publication Number Publication Date
WO1993018163A2 WO1993018163A2 (en) 1993-09-16
WO1993018163A3 true WO1993018163A3 (en) 1993-11-11

Family

ID=25309927

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/002355 WO1993018163A2 (en) 1990-05-11 1993-03-12 Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria

Country Status (10)

Country Link
EP (1) EP0646176B1 (en)
JP (1) JP3626181B2 (en)
AT (1) ATE269904T1 (en)
AU (1) AU674311B2 (en)
CA (1) CA2131995A1 (en)
DE (1) DE69333560T2 (en)
DK (1) DK0646176T3 (en)
ES (1) ES2224097T3 (en)
HU (1) HU220420B (en)
WO (1) WO1993018163A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
HU220420B (en) * 1992-03-13 2002-01-28 The Rockefeller University A hybrid surface protein of gram positive bacteria, method and kit for detecting of infection
US5516637A (en) * 1994-06-10 1996-05-14 Dade International Inc. Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage
AUPM885194A0 (en) * 1994-10-14 1994-11-10 Council Of The Queensland Institute Of Medical Research, The Synthetic peptides and vaccines comprising same
GB9518323D0 (en) 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US7101692B2 (en) 1999-04-15 2006-09-05 The Regents Of The University Of California Identification of sortase gene
CA2365523A1 (en) 1999-04-15 2000-10-26 The Regents Of The University Of California Identification of sortase gene
EP1171098A4 (en) 1999-04-16 2003-04-02 Osel Inc A method for improving the half-life of soluble viral-specific ligands on mucosal membranes
US20100086485A1 (en) * 2006-01-17 2010-04-08 Heiko Apfel Influenza vaccine
IL208820A0 (en) * 2010-10-19 2011-01-31 Rachel Teitelbaum Biologic female contraceptives
US9925257B2 (en) 2011-09-23 2018-03-27 Forsyth Dental Infirmary For Children Vaccine and therapeutic delivery system
WO2013044059A2 (en) 2011-09-23 2013-03-28 Forsyth Dental Infirmary For Children A vaccine and therapeutic delivery system
MA41020A (en) 2014-11-25 2017-10-03 Evelo Biosciences Inc PROBIOTIC AND PREBIOTIC COMPOSITIONS, AND THEIR METHODS OF USE FOR MODULATION OF THE MICROBIOME

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015872A1 (en) * 1989-06-21 1990-12-27 The Rockefeller University Recombinant poxvirus and streptococcal m protein vaccine
WO1992020805A1 (en) * 1991-05-13 1992-11-26 Pierre Fabre Medicament Recombinant dna coding for signal peptide, selective interacting polypeptide and membrane anchoring sequence

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU220420B (en) * 1992-03-13 2002-01-28 The Rockefeller University A hybrid surface protein of gram positive bacteria, method and kit for detecting of infection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015872A1 (en) * 1989-06-21 1990-12-27 The Rockefeller University Recombinant poxvirus and streptococcal m protein vaccine
WO1992020805A1 (en) * 1991-05-13 1992-11-26 Pierre Fabre Medicament Recombinant dna coding for signal peptide, selective interacting polypeptide and membrane anchoring sequence

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BIO/TECHNOLOGY vol. 9, no. 12, December 1991, NATURE AMERICA, INC., NEW YORK, US pages 1369 - 1372 P. FUCHS ET AL. 'Targeting recombinant antibodies to the surface of Escherichia coli: Fusion to a peptidoglycan associated lipoprotein' *
GENE vol. 104, no. 2, 15 August 1991, ELSEVIER SCIENCE, AMSTERDAM, NL; pages 147 - 153 F. BREITLING ET AL. 'A surface expression vector for antibody screening' *
INFECTION AND IMMUNITY vol. 60, no. 5, May 1992, AM.SOC.MICROBIOL.,WASHINGTON,DC,US; pages 1902 - 1907 G. POZZI ET AL. 'Delivery and expression of a heterologous antigen on the surface of streptococci' *
NATURE vol. 351, 6 June 1991, MACMILLAN JOURNALS LTD,LONDON,UK; pages 456 - 460 C.K. STOVER ET AL. 'New use of BCG for recombinant vaccines' cited in the application the whole document *
SCIENCE vol. 244, 7 April 1989, AAAS, WASHINGTON, DC, US; pages 70 - 72 S.M.C. NEWTON ET AL. 'Immune response to cholera toxin epitope inserted in Salmonella flagellin' *
XI lancefield international symposium on Streptococci and streptococcal diseases, Siena, Italy, September 10-14, 1990; p 350- 352; the whole document *
ZENTRALBLATT FUER BAKTERIOLOGIE SUPPLEMENT, 22 (INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY) 1992, GUSTAV FISCHER VERLAG;STUTTGART,BRD; OREFICI, G. (ED.). 'NEW PERSPECTIVES ON STREPTOCOCCI AND STREPTOCOCCAL INFECTIONS' *

Also Published As

Publication number Publication date
DK0646176T3 (en) 2004-11-08
JP3626181B2 (en) 2005-03-02
AU674311B2 (en) 1996-12-19
EP0646176A1 (en) 1995-04-05
AU3919993A (en) 1993-10-05
ATE269904T1 (en) 2004-07-15
HU9402575D0 (en) 1994-11-28
WO1993018163A2 (en) 1993-09-16
JP2002509421A (en) 2002-03-26
HU220420B (en) 2002-01-28
EP0646176B1 (en) 2004-06-23
DE69333560T2 (en) 2006-07-13
CA2131995A1 (en) 1993-09-16
ES2224097T3 (en) 2005-03-01
DE69333560D1 (en) 2004-07-29
HUT70306A (en) 1995-09-28

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