DE69333560D1 - TRANSPORT AND EXPRESSION OF A HYBRID SURFACE PROTEIN ON THE SURFACE OF GRAMPOSITIVE BACTERIA - Google Patents
TRANSPORT AND EXPRESSION OF A HYBRID SURFACE PROTEIN ON THE SURFACE OF GRAMPOSITIVE BACTERIAInfo
- Publication number
- DE69333560D1 DE69333560D1 DE69333560T DE69333560T DE69333560D1 DE 69333560 D1 DE69333560 D1 DE 69333560D1 DE 69333560 T DE69333560 T DE 69333560T DE 69333560 T DE69333560 T DE 69333560T DE 69333560 D1 DE69333560 D1 DE 69333560D1
- Authority
- DE
- Germany
- Prior art keywords
- expression
- transport
- hybrid
- grampositive bacteria
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000018697 Membrane Proteins Human genes 0.000 title abstract 2
- 108010052285 Membrane Proteins Proteins 0.000 title abstract 2
- 241000192125 Firmicutes Species 0.000 title 1
- 241000894006 Bacteria Species 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/385—Haptens or antigens, bound to carriers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
- A61K2039/6031—Proteins
- A61K2039/6068—Other bacterial proteins, e.g. OMP
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Crystallography & Structural Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Process is described for the delivery and expression of hybrid surface proteins to the surface of bacteria. The transformed bacteria are useful as vaccines, for the delivery of other active peptides to animal hosts, as diagnostic reagents and for other purposes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US85108292A | 1992-03-13 | 1992-03-13 | |
US851082 | 1992-03-13 | ||
PCT/US1993/002355 WO1993018163A2 (en) | 1992-03-13 | 1993-03-12 | Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69333560D1 true DE69333560D1 (en) | 2004-07-29 |
DE69333560T2 DE69333560T2 (en) | 2006-07-13 |
Family
ID=25309927
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69333560T Expired - Fee Related DE69333560T2 (en) | 1992-03-13 | 1993-03-12 | TRANSPORT AND EXPRESSION OF A HYBRID SURFACE PROTEIN ON THE SURFACE OF GRAMPOSITIVE BACTERIA |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0646176B1 (en) |
JP (1) | JP3626181B2 (en) |
AT (1) | ATE269904T1 (en) |
AU (1) | AU674311B2 (en) |
CA (1) | CA2131995A1 (en) |
DE (1) | DE69333560T2 (en) |
DK (1) | DK0646176T3 (en) |
ES (1) | ES2224097T3 (en) |
HU (1) | HU220420B (en) |
WO (1) | WO1993018163A2 (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5821088A (en) * | 1990-05-11 | 1998-10-13 | Siga Pharmaceuticals, Inc. | Use of gram-positive bacteria to express recombinant proteins |
JP3626181B2 (en) * | 1992-03-13 | 2005-03-02 | ザ ロックフェラー ユニバーシティー | Supply and expression of hybrid surface proteins on the surface of Gram-positive bacteria |
US5516637A (en) * | 1994-06-10 | 1996-05-14 | Dade International Inc. | Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage |
AUPM885194A0 (en) * | 1994-10-14 | 1994-11-10 | Council Of The Queensland Institute Of Medical Research, The | Synthetic peptides and vaccines comprising same |
GB9518323D0 (en) | 1995-09-07 | 1995-11-08 | Steidler Lothar | Materials and methods relating to the attachment and display of substances on cell surfaces |
US7101692B2 (en) | 1999-04-15 | 2006-09-05 | The Regents Of The University Of California | Identification of sortase gene |
JP2003506011A (en) | 1999-04-15 | 2003-02-18 | ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニア | Identification of sortase gene |
CN1355690A (en) | 1999-04-16 | 2002-06-26 | 奥赛尔股份有限公司 | Method for improving half-life of soluble viral-specific ligands on mucosal membranes |
US20100086485A1 (en) * | 2006-01-17 | 2010-04-08 | Heiko Apfel | Influenza vaccine |
IL208820A0 (en) * | 2010-10-19 | 2011-01-31 | Rachel Teitelbaum | Biologic female contraceptives |
US9925257B2 (en) | 2011-09-23 | 2018-03-27 | Forsyth Dental Infirmary For Children | Vaccine and therapeutic delivery system |
US9376686B2 (en) | 2011-09-23 | 2016-06-28 | Forsyth Dental Infirmary For Children | Vaccine and therapeutic delivery system |
MA41020A (en) | 2014-11-25 | 2017-10-03 | Evelo Biosciences Inc | PROBIOTIC AND PREBIOTIC COMPOSITIONS, AND THEIR METHODS OF USE FOR MODULATION OF THE MICROBIOME |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE240396T1 (en) * | 1989-06-21 | 2003-05-15 | Univ Rockefeller | RECOMBINANT POXVIRUS AND STREPTOCOCCI CONTAINING AN M-PROTEIN |
SE9101433D0 (en) * | 1991-05-13 | 1991-05-13 | Marianne Hansson | RECOMBINANT DNA SEQUENCE AND ITS USE |
JP3626181B2 (en) * | 1992-03-13 | 2005-03-02 | ザ ロックフェラー ユニバーシティー | Supply and expression of hybrid surface proteins on the surface of Gram-positive bacteria |
-
1993
- 1993-03-12 JP JP51605193A patent/JP3626181B2/en not_active Expired - Fee Related
- 1993-03-12 DE DE69333560T patent/DE69333560T2/en not_active Expired - Fee Related
- 1993-03-12 WO PCT/US1993/002355 patent/WO1993018163A2/en active IP Right Grant
- 1993-03-12 DK DK93908349T patent/DK0646176T3/en active
- 1993-03-12 CA CA002131995A patent/CA2131995A1/en not_active Abandoned
- 1993-03-12 AT AT93908349T patent/ATE269904T1/en not_active IP Right Cessation
- 1993-03-12 HU HU9402575A patent/HU220420B/en not_active IP Right Cessation
- 1993-03-12 AU AU39199/93A patent/AU674311B2/en not_active Ceased
- 1993-03-12 EP EP93908349A patent/EP0646176B1/en not_active Expired - Lifetime
- 1993-03-12 ES ES93908349T patent/ES2224097T3/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69333560T2 (en) | 2006-07-13 |
JP3626181B2 (en) | 2005-03-02 |
AU3919993A (en) | 1993-10-05 |
EP0646176B1 (en) | 2004-06-23 |
ATE269904T1 (en) | 2004-07-15 |
HU220420B (en) | 2002-01-28 |
HUT70306A (en) | 1995-09-28 |
CA2131995A1 (en) | 1993-09-16 |
JP2002509421A (en) | 2002-03-26 |
WO1993018163A3 (en) | 1993-11-11 |
DK0646176T3 (en) | 2004-11-08 |
WO1993018163A2 (en) | 1993-09-16 |
HU9402575D0 (en) | 1994-11-28 |
EP0646176A1 (en) | 1995-04-05 |
ES2224097T3 (en) | 2005-03-01 |
AU674311B2 (en) | 1996-12-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |