WO1993013204A3 - Mittel zur stimulierung der teilungsaktivität tierischer zellen - Google Patents

Mittel zur stimulierung der teilungsaktivität tierischer zellen Download PDF

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Publication number
WO1993013204A3
WO1993013204A3 PCT/EP1992/002929 EP9202929W WO9313204A3 WO 1993013204 A3 WO1993013204 A3 WO 1993013204A3 EP 9202929 W EP9202929 W EP 9202929W WO 9313204 A3 WO9313204 A3 WO 9313204A3
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WO
WIPO (PCT)
Prior art keywords
agent
cell division
nucleotides
stimulating animal
animal cell
Prior art date
Application number
PCT/EP1992/002929
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English (en)
French (fr)
Other versions
WO1993013204A2 (de
Inventor
Michael Strauss
Andre Lieber
Original Assignee
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft filed Critical Max Planck Gesellschaft
Publication of WO1993013204A2 publication Critical patent/WO1993013204A2/de
Publication of WO1993013204A3 publication Critical patent/WO1993013204A3/de
Priority to US08/511,836 priority Critical patent/US5831067A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Die Erfindung betrifft ein Mittel zur Stimulierung der Teilungsaktivität tierischer und menschlicher Zellen. Anwendungsgebiete sind die biologisch-medizinische Grundlagenforschung, die Biotechnologie und die pharmazeutische Industrie. Das erfindungsgemäße Mittel ist durch Nukleotidsequenzen mit komplementärer Sequenz (antisense) zu bestimmten Bereichen der mRNA von Tumorsuppressorgenen oder anderen negativ auf die Zellteilungsaktivität wirkenden Genen gekennzeichnet. Als Nukleotidsequenz werden bevorzugt synthetische Oligonukleotide einer Länge von mindestens 15 Nukleotiden, bevorzugt 18 - 21 Nukleotide, eingesetzt. Das Mittel wird dem Kulturmedium in einer Menge von 5-30 νM zugesetzt, wobei die Konzentration während der Kultivierung im wesentlichen konstant bleiben muß.
PCT/EP1992/002929 1991-12-18 1992-12-17 Mittel zur stimulierung der teilungsaktivität tierischer zellen WO1993013204A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US08/511,836 US5831067A (en) 1991-12-18 1995-08-07 Agent for stimulating animal cell division

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4142452.2 1991-12-18
DE19914142452 DE4142452A1 (de) 1991-12-18 1991-12-18 Mittel zur stimulierung der teilungsaktivitaet tierischer zellen

Publications (2)

Publication Number Publication Date
WO1993013204A2 WO1993013204A2 (de) 1993-07-08
WO1993013204A3 true WO1993013204A3 (de) 1993-08-05

Family

ID=6447798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1992/002929 WO1993013204A2 (de) 1991-12-18 1992-12-17 Mittel zur stimulierung der teilungsaktivität tierischer zellen

Country Status (3)

Country Link
EP (1) EP0572641A1 (de)
DE (1) DE4142452A1 (de)
WO (1) WO1993013204A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759776A (en) * 1995-06-05 1998-06-02 California Pacific Medical Center Targets for breast cancer diagnosis and treatment
US5776683A (en) * 1996-07-11 1998-07-07 California Pacific Medical Center Methods for identifying genes amplified in cancer cells
US5830723A (en) * 1996-08-13 1998-11-03 Regents Of The University Of Minnesota Method for immortalizing chicken cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009180A1 (en) * 1989-02-15 1990-08-23 Board Of Regents, The University Of Texas System Methods and compositions for treatment of cancer using oligonucleotides
WO1992005272A1 (en) * 1990-09-17 1992-04-02 The Regents Of The University Of California Method and composition for controlling proliferation of cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009180A1 (en) * 1989-02-15 1990-08-23 Board Of Regents, The University Of Texas System Methods and compositions for treatment of cancer using oligonucleotides
WO1992005272A1 (en) * 1990-09-17 1992-04-02 The Regents Of The University Of California Method and composition for controlling proliferation of cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ERICKSON, R.P. & IZANT, J.G. 'gene regulation: biology of antisense RNA and DNA' 1992 , RAVEN PRESS, LTD. , NEW YORK Seiten 317-328; SCHLINGENSIEPEN, K.-H. & BRYSCH, W.:' Phosphorothioate oligomers: inhibitors of oncogene expression in tumor cells and tools for gene function analysis' *
ONCOGENE Bd. 5, 1990, Seiten 973 - 980 MERCER, W.E. ET AL. 'Wild type human p53 is antiproliferative in SV40-transformed hamster cells' *
ONCOGENE Bd. 7, Nr. 4, April 1992, Seiten 769 - 773 STRAUSS, M. ET AL. 'Stimulation of cell division and fibroblast focus formation by antisense repression of retinoblastoma protein synthesis' *
PROCEEDINGS OF THE 82nd ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH; 15-18 Mai 1991; HOUSTON, TEXAS Bd. 32, März 91; Seite 303, Nummer 1799; SCHLINGENSIEPEN, K.-H. & BRYSCH, W.: 'Proto-oncogenes c-jun and jun-b have opposite effects on cell proliferation: a gene suppression study using antisense phosphorothioate oligodeoxynucleotides' *

Also Published As

Publication number Publication date
DE4142452A1 (de) 1993-06-24
WO1993013204A2 (de) 1993-07-08
EP0572641A1 (de) 1993-12-08

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