WO1993011225A1 - Culture de cellules osseuses - Google Patents

Culture de cellules osseuses Download PDF

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Publication number
WO1993011225A1
WO1993011225A1 PCT/GB1992/002185 GB9202185W WO9311225A1 WO 1993011225 A1 WO1993011225 A1 WO 1993011225A1 GB 9202185 W GB9202185 W GB 9202185W WO 9311225 A1 WO9311225 A1 WO 9311225A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
bone
asc
culture
primary
Prior art date
Application number
PCT/GB1992/002185
Other languages
English (en)
Inventor
Martin James Ogilvie Francis
Jon Nicholas Beresford
Stephen Ellis Graves
Original Assignee
Isis Innovation Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation Limited filed Critical Isis Innovation Limited
Publication of WO1993011225A1 publication Critical patent/WO1993011225A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Abstract

L'invention concerne un procédé pour maintenir des cellules osseuses ou des cellules ostéoformatrices en culture et consistant à produire des cellules, au stade de culture primaire, avec une source à action prolongée d'acide ascorbique L 2-phosphate. De préférence, au premier stade de passage, les cellules en culture sont introduites dans le corps comme une allogreffe, une autogreffe ou une hétérogreffe, avec le support d'une matrice biocompatible ou sous la forme d'une suspension.
PCT/GB1992/002185 1991-11-26 1992-11-26 Culture de cellules osseuses WO1993011225A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9125052.2 1991-11-26
GB919125052A GB9125052D0 (en) 1991-11-26 1991-11-26 Culture of bone cells

Publications (1)

Publication Number Publication Date
WO1993011225A1 true WO1993011225A1 (fr) 1993-06-10

Family

ID=10705214

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1992/002185 WO1993011225A1 (fr) 1991-11-26 1992-11-26 Culture de cellules osseuses

Country Status (3)

Country Link
AU (1) AU2951892A (fr)
GB (1) GB9125052D0 (fr)
WO (1) WO1993011225A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997014783A1 (fr) * 1995-10-20 1997-04-24 North Shore University Hospital Research Corporation Reparation osseuse par modification tissulaire faisant appel a des cellules periostiques cultivees
WO1999035242A1 (fr) * 1998-01-12 1999-07-15 Betagene, Inc. Milieu de culture de cellules neuroendocrines
US6152964A (en) * 1996-03-01 2000-11-28 Isotis B.V. Method for in vitro production of bone
EP2451963A1 (fr) * 2009-07-10 2012-05-16 CSL Limited Procédé d'augmentation du rendement de l'expression de protéines dépendantes de la vitamine k
US20130267026A1 (en) * 2002-06-07 2013-10-10 P Tech, Llc Methods of building a body portion

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0282746A1 (fr) * 1987-02-19 1988-09-21 Takeda Chemical Industries, Ltd. Procédé de préparation de tissu cultivé de manière artificielle
EP0339607A2 (fr) * 1988-04-29 1989-11-02 Samuel Dr. Itay Composition pour la réparation de cartilage et d'os et procédé pour sa préparation comme implant de tissu du squelette

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0282746A1 (fr) * 1987-02-19 1988-09-21 Takeda Chemical Industries, Ltd. Procédé de préparation de tissu cultivé de manière artificielle
EP0339607A2 (fr) * 1988-04-29 1989-11-02 Samuel Dr. Itay Composition pour la réparation de cartilage et d'os et procédé pour sa préparation comme implant de tissu du squelette

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BONE vol. 11, no. 5, 1990, NEW YORK, N.Y., US page 378 S.E. GRAVES ET AL. 'EFFECTS OF L-ASCORBIC ACID 2-PHOSPHATE ON HUMAN BONE DERIVED CELLS.' cited in the application *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997014783A1 (fr) * 1995-10-20 1997-04-24 North Shore University Hospital Research Corporation Reparation osseuse par modification tissulaire faisant appel a des cellules periostiques cultivees
US6152964A (en) * 1996-03-01 2000-11-28 Isotis B.V. Method for in vitro production of bone
WO1999035242A1 (fr) * 1998-01-12 1999-07-15 Betagene, Inc. Milieu de culture de cellules neuroendocrines
US20130267026A1 (en) * 2002-06-07 2013-10-10 P Tech, Llc Methods of building a body portion
US10294455B2 (en) * 2002-06-07 2019-05-21 P Tech, Llc Methods of building a body portion
US11613731B2 (en) 2002-06-07 2023-03-28 P Tech, Llc Scaffold and method for implanting cells
EP2451963A1 (fr) * 2009-07-10 2012-05-16 CSL Limited Procédé d'augmentation du rendement de l'expression de protéines dépendantes de la vitamine k
EP2451963A4 (fr) * 2009-07-10 2013-01-23 Csl Ltd Procédé d'augmentation du rendement de l'expression de protéines dépendantes de la vitamine k
US9212214B2 (en) 2009-07-10 2015-12-15 Csl Limited Methods of increasing the expression yield of vitamin K-dependent proteins

Also Published As

Publication number Publication date
AU2951892A (en) 1993-06-28
GB9125052D0 (en) 1992-01-22

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