WO1993001304A1 - Peptides synthetiques relatifs a des proteines d'enveloppe du vif - Google Patents
Peptides synthetiques relatifs a des proteines d'enveloppe du vif Download PDFInfo
- Publication number
- WO1993001304A1 WO1993001304A1 PCT/US1992/005689 US9205689W WO9301304A1 WO 1993001304 A1 WO1993001304 A1 WO 1993001304A1 US 9205689 W US9205689 W US 9205689W WO 9301304 A1 WO9301304 A1 WO 9301304A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- phe
- gln
- peptide
- glu
- ala
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Definitions
- This invention relates to non-naturally occurring or synthetic peptides which mimic an antigenic site on the surface of feline immunodeficiency virus (FIV) envelope protein.
- FV feline immunodeficiency virus
- feline immunodeficiency virus A retrovirus termed feline immunodeficiency virus (FIV) is now known to be the etiological agent in feline acquired immunodeficiency syndrome (FAIDS). Testing for FIV is important in diagnosing exposure to the virus in order to to limit the spread of infection.
- FAIDS feline acquired immunodeficiency syndrome
- Detection of exposure to FIV is accomplished by measuring levels of FIV antibodies in serum, plasma, whole blood or saliva.
- inactivated crude or purified viral protein from lysates of FIV-infected cells are used to coat a solid phase.
- Biological samples suspected of containing FIV antibodies are incubated with the solid phase and after an
- anti-feline antibody tagged with a detectable label is added to the solid phase.
- the label which may be an enzyme, radioisotope or fluorescent molecule is measured to determine the presence of FIV antibodies in the sample.
- the difficulty with using viral lysates as a source of FIV antigens is the high level of
- This invention provides a highly sensitive and accurate detection or diagnostic procedure that does not require the use of virus or lysates thereof as a test reagent.
- a polypeptide test reagent prepared by chemical means is used to detect the presence of antibodies to FIV in body fluids.
- test reagent comprising a peptide having about twenty seven amino acids arranged in a specific sequence, preferably prepared by solid phase
- the 27mer peptide is useful in a highly sensitive and accurate method for the detection of antibodies to FIV in serum.
- a shorter 13mer peptide contained within the 27mer is similarly useful.
- Peptides having specific immunoreactivity to FIV antibodies are selected from the group consisting of peptides containing from twelve to twenty seven amino acids, the sequence of which corresponds to a part or the entirety of the following sequence
- the highly sensitive and accurate method for the detection of antibodies to FIV in serum comprises the following steps:
- a solid phase such as polystyrene
- a buffered solution containing from about 0.5 ug/ml to about 20 ug/ml of peptide, in an appropriate solvent such as phosphate buffered or borate buffered saline.
- a detectable label which may be an enzyme, radioisotope or fluorescent molecule.
- Horseradish peroxidase was activated by periodate oxidation (20 mM sodium metaperiodate) for two hours at 25°C. Peptide was added to the activate enzyme and allowed to react for two hours at 25 °C and the resulting conjugate was stabilized by sodium
- conjugation ratio of peptide to enzyme of 15-35 The conjugated peptide is diluted for use into a solution containing 10 mM phosphate, 6% (w/v) bovine serum albumin and 0.1% (v/v) Tween 20.
- the working peptide is diluted for use into a solution containing 10 mM phosphate, 6% (w/v) bovine serum albumin and 0.1% (v/v) Tween 20.
- conjugate concentration is from about 0.5 ug/ml to about 10 ug/ml.
- IFA immunofluorescence assay
Abstract
L'invention concerne des peptides nouveaux et utiles dans des tests de diagnostic du virus de l'immunodéficience féline (VIF).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US72813491A | 1991-07-10 | 1991-07-10 | |
US728,134 | 1991-07-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1993001304A1 true WO1993001304A1 (fr) | 1993-01-21 |
Family
ID=24925565
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1992/005689 WO1993001304A1 (fr) | 1991-07-10 | 1992-07-09 | Peptides synthetiques relatifs a des proteines d'enveloppe du vif |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO1993001304A1 (fr) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994006471A1 (fr) * | 1992-09-21 | 1994-03-31 | Mallinckrodt Veterinary,Inc. | Vaccins destines a lutter contre le virus immunodeficitaire felin |
EP0602046A1 (fr) * | 1991-06-14 | 1994-06-22 | St. Vincent's Institute Of Medical Research | Detection des virus de l'immunodeficience chez felis |
FR2721031A1 (fr) * | 1994-06-09 | 1995-12-15 | Centre Nat Rech Scient | Fragment peptidique spécifique du virus de l'immunodéficience féline (VIF) et son utilisation comme réactif de diagnostic. |
FR2771011A1 (fr) * | 1997-11-17 | 1999-05-21 | Hippocampe | Obtention de vaccins destines a prevenir les effets pathogenes associes a une infection retrovirale |
US7201903B2 (en) | 2003-09-11 | 2007-04-10 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7285278B2 (en) | 2004-06-30 | 2007-10-23 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7285272B2 (en) | 2003-12-18 | 2007-10-23 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7291338B2 (en) | 2005-03-09 | 2007-11-06 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7335360B2 (en) | 2004-06-30 | 2008-02-26 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7348136B2 (en) | 2004-02-19 | 2008-03-25 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US8809004B2 (en) | 2010-04-02 | 2014-08-19 | Idexx Laboratories, Inc. | Detection of feline immunodeficiency virus |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5037753A (en) * | 1987-08-26 | 1991-08-06 | The Regents Of The University Of California | Feline t-lymphotropic lentivirus |
-
1992
- 1992-07-09 WO PCT/US1992/005689 patent/WO1993001304A1/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5037753A (en) * | 1987-08-26 | 1991-08-06 | The Regents Of The University Of California | Feline t-lymphotropic lentivirus |
US5037753B1 (en) * | 1987-08-26 | 1994-03-15 | The Regents Of The University Of California | Feline t-lymphotropic lentivirus |
US5037753B2 (en) * | 1987-08-26 | 1998-06-16 | Univ California | Feline t-lymphotrophic lentivirus |
Non-Patent Citations (7)
Title |
---|
JOURNAL OF GENERAL VIROLOGY, Volume 71, issued 1990, H.F. EGBERINK et al., "Intracellular Proteins of Feline Immunodeficiency Virus and Their Antigenic Relationship with Equine Infectious Anaemia Virus Proteins", pages 739-743. * |
JOURNAL OF GENERAL VIROLOGY, Volume 71, issued 1990, R. STEINMAN et al., "Biochemical and Immunological Characterization of the Major Structural Proteins of Feline Immunodeficiency Virus", pages 701-706. * |
JOURNAL OF VIROLOGY, Volume 65, No. 3, issued March 1991, E.B. STEPHENS et al., "Processing of the Glycoprotein of Feline Immunodeficiency Virus: Effect of Inhibitors of Glycosylation", pages 1114-1123. * |
JOURNAL OF VIROLOGY, Volume 65, No. 8, issued August 1991, T. KIYOMASU et al., "Identification of Feline Immunodeficiency Virus Rev Gene Activity", pages 4539-4542. * |
MOLECULAR IMMUNOLOGY, Volume 29, No. 5, issued 1992, A.AVRAMEAS, "Localization of Three Epitopes of the Env Protein of Feline Immunodeficiency Virus", pages 565-572. * |
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, Volume 86, issued April 1989, R.A. OLMSTED et al., "Molecular Cloning of Feline Immunodeficiency Virus", pages 2448-2452. * |
VIROLOGY, Volume 183, issued 1991, S. MORIKAWA et al., "Analysis of the Requirements for the Synthesis of Virus-Like Particles by Feline Immunodeficiency Virus Gag Using Baculovirus Vectors", pages 288-297. * |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0602046A1 (fr) * | 1991-06-14 | 1994-06-22 | St. Vincent's Institute Of Medical Research | Detection des virus de l'immunodeficience chez felis |
EP0602046A4 (fr) * | 1991-06-14 | 1995-07-19 | St Vincents Inst Med Res | Detection des virus de l'immunodeficience chez les mammiferes. |
US5591572A (en) * | 1991-06-14 | 1997-01-07 | St. Vincents Institute Of Medical Research Limited | Detection of mammalian immunodeficiency viruses |
WO1994006471A1 (fr) * | 1992-09-21 | 1994-03-31 | Mallinckrodt Veterinary,Inc. | Vaccins destines a lutter contre le virus immunodeficitaire felin |
FR2721031A1 (fr) * | 1994-06-09 | 1995-12-15 | Centre Nat Rech Scient | Fragment peptidique spécifique du virus de l'immunodéficience féline (VIF) et son utilisation comme réactif de diagnostic. |
EP0688790A1 (fr) * | 1994-06-09 | 1995-12-27 | Centre National De La Recherche Scientifique | Fragment peptidique spécifique du virus de l'immunodéficience féline (VIF), et son utilisation comme réactif de diagnostic |
US5648209A (en) * | 1994-06-09 | 1997-07-15 | Centre National De La Recherche Scientifique-Cnrs | Specific peptide fragment of the feline immunodeficiency virus (FIV), and its use as a diagnostic reagent |
US7829683B2 (en) | 1997-11-17 | 2010-11-09 | Lomastar Technologies Sarl | Polynucleotides encoding modified HIV-1 gp41 membrane polypeptides |
FR2771011A1 (fr) * | 1997-11-17 | 1999-05-21 | Hippocampe | Obtention de vaccins destines a prevenir les effets pathogenes associes a une infection retrovirale |
WO1999025377A1 (fr) * | 1997-11-17 | 1999-05-27 | Hippocampe | Obtention de vaccins destines a prevenir les effets pathogenes associes a une infection retrovirale |
US6455265B1 (en) | 1997-11-17 | 2002-09-24 | Mymetics S.A. | Method for obtaining vaccines for preventing the pathogenic effects related to a retroviral infection |
US7253270B2 (en) | 1997-11-17 | 2007-08-07 | Mymetics Sa | Polynucleotide encoding a mutated HIV gp41 polypeptide |
US7201903B2 (en) | 2003-09-11 | 2007-04-10 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7776546B2 (en) | 2003-09-11 | 2010-08-17 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7285272B2 (en) | 2003-12-18 | 2007-10-23 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7348136B2 (en) | 2004-02-19 | 2008-03-25 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7335360B2 (en) | 2004-06-30 | 2008-02-26 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7285278B2 (en) | 2004-06-30 | 2007-10-23 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US7291338B2 (en) | 2005-03-09 | 2007-11-06 | Idexx Laboratories, Inc. | Method and device for detecting feline immunodeficiency virus |
US8809004B2 (en) | 2010-04-02 | 2014-08-19 | Idexx Laboratories, Inc. | Detection of feline immunodeficiency virus |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230296601A1 (en) | Novel coronavirus antibody detection kit based on magnetic particle chemiluminescence | |
CA2661875C (fr) | Procede combinatoire de detection d'anticorps anti-virus de l'hepatite c et d'antigenes du virus de l'hepatite c | |
CA1303494C (fr) | Dosage immunoenzymatique permettant de deceler simultanement des antigenes et des anticorps chez l'humain | |
EP0202890A2 (fr) | ELISA-compétitif pour détecter les anticorps | |
EP0111211A1 (fr) | Immunoessai pour protéines et fragments de protéines non enzymatiquement glucosylés - un indice de la glycémie | |
US5625034A (en) | Core antigen protein of hepatitis C virus, and diagnostic method and kit using the same | |
WO1993001304A1 (fr) | Peptides synthetiques relatifs a des proteines d'enveloppe du vif | |
US6379886B1 (en) | Diagnostic regeant for hepatitis C virus infection | |
Masihi et al. | Enzyme-linked immunosorbent assay for the detection of influenza type-specific antibodies | |
EP0514509B1 (fr) | Composition comprenant des antigenes conjugues a une activite enzymatique, et destinee aux necessaires a usage prive et epidemiologique de diagnostic immunologique, notamment de la maladie de chagas, et procede de preparation de ladite composition | |
JP3374101B2 (ja) | Env/gagポリペプチドマーカーを使用するネコ免疫不全ウイルス感染の診断 | |
CN101523216B (zh) | 人类免疫缺陷病毒系列蛋白抗体测定方法 | |
CA1279818C (fr) | Test pour la detection des anticorps contre certains micro-organismes | |
WO2004070387A1 (fr) | Procede ameliore de detection d'anticorps vhc dans un dosage combine ou dans un seul dosage d'anticorps | |
JP2003202345A (ja) | Hcvコア抗原及び抗体の同時検出試薬 | |
EP0313986B1 (fr) | Dosages immunologiques utilisant des antigènes produits dans des organismes hétérologues | |
Graddon et al. | Single antibody dot immunoassay—a simple technique for rapid detection of a plant virus | |
EP0443880B1 (fr) | Méthode d'analyse du virus de l'anémie infectieuse équine (EIA-Virus) | |
Obwaller et al. | An enzyme-linked immunosorbent assay with whole trophozoites of Toxoplasma gondii from serum-free tissue culture for detection of specific antibodies | |
WO1998058259A1 (fr) | Stabilisation de polypeptides a utiliser dans des procedes de dosage immunologique | |
US6596846B2 (en) | Method and composition for the diagnosis of equine infectious anemia virus disease by using the recombinant capsid protein virus (p26) | |
KR910007962A (ko) | Hiv 관련 펩티드 | |
Rho et al. | Characterization of reverse transcriptase from feline leukemia virus by radioimmunoassay | |
CA1276103C (fr) | Composant complementaire clq conjugue a une substance | |
Hilbink et al. | A comparison of two enzyme linked immunosorbent assays for enzootic bovine leucosis serology |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE |
|
122 | Ep: pct application non-entry in european phase |