WO1992008794A1 - Plantes resistantes aux herbicides - Google Patents
Plantes resistantes aux herbicides Download PDFInfo
- Publication number
- WO1992008794A1 WO1992008794A1 PCT/GB1991/001972 GB9101972W WO9208794A1 WO 1992008794 A1 WO1992008794 A1 WO 1992008794A1 GB 9101972 W GB9101972 W GB 9101972W WO 9208794 A1 WO9208794 A1 WO 9208794A1
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- herbicide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
Definitions
- This invention relates to herbicide resistant maize plants and mutant gene sequences providing said resistance.
- the invention further relates to plants containing said gene sequences and the seed and progeny of the plants.
- the purpose in providing crop plants which resist the action of a herbicide is to facilitate the destruction of weeds growing between the plants by the overall application of a herbicidally effective concentration of a herbicide which would destroy the crop plant in its normal, that is herbicide sensitive, state.
- Such resistant plants are also useful for use in a locus of any short term carry-over of herbicide from a previous crop.
- Methods are known by which populations of plants may be obtained which contain a great number of random mutations. Such methods include tissue culture techniques where spontaneous somaclonal variation occurs in the presence or absence of a mutagen. By applying to the cultures some form of selection pressure it is possible to recover cells which resist that pressure. Depending * on the plant species it is sometimes possible to regenerate whole plants from the resistant cells.
- tissue culture selection methods have been used in the past to select for resistance to herbicides.
- European Patent Application Number 257,993 discloses a general scheme of mutations to the gene encoding acetolactate synthase which are said to confer resistance to the sulphonylurea class of herbicides.
- An object of the present invention is to provide genetic material for using in imparting herbicide resistance to plants.
- a plant DNA comprising the Apal to Stul or the Xhol to Stul restriction fragment shown in Figure 24 or the Xhol to NspHl restriction fragment shown in Figure 25, and variations in said DNAs as permitted by the degeneracy of the genetic code.
- Plants particularly maize plants, which contain said DNAs stably integrated within their genomes.
- the invention also provides seeds and progeny of the said plant which have been produced by crossing of the plants of this invention with other maize plant lines.
- mutant DNAs of this invention may be isolated from seeds deposited on 8th May 1989 with the National Collection of Industrial & Marine Bacteria, Aberdeen, United Kingdom, under the Accession Numbers NCIMB 40137 and 40136. Each of these deposits is a genetic mixture of maize seeds, segregating mutant and non-mutant seeds. The mutants are heterozygous for the gene conferring herbicide resistance. Mutant plants may be derived from these seeds by growing under the conditions described below under the heading "Screening" in the presence of the herbicide imazethapyr.
- the plants of this invention are known to be resistant to certain members of the imidazolinone family of herbicides, for example, imazethapyr [5- ethyl-2-(5-iso- propyl-5-methyl-4-oxo-2-imidazolin- 2-yl) nicotinic acid: (Trade Mark PURSUIT, American Cyanamid)].
- Cross resistance to herbicides of the sulphonylurea family, chlorsulfuron, for example might have been expected on the basis of reports in the literature and the fact that acetolactate synthase is the site of action of both the imidazolinones and the sulphonylurea ⁇ .
- the resistance conferred by the genes of this invention is specific to the imidazolinones in general and PURSUIT particularly. Any tolerance which the plants may have of the sulphonylurea ⁇ is insignificant.
- tissue culture selection method whole plants have to be regenerated from the tissue and grown to maturity before any indication of the performance of the progeny under field application rates of the herbicide can be obtained.
- selection is made directly on the plants under concentrations of herbicide which are comparable to those which are recommended for normal weed-killing activity in the field.
- Selection on the M- generation, as with the tissue culture method selects ' recessive mutations as well as dominant traits. Dominance of a desirable trait is generally viewed as more useful and easier to handle in a breeding programme especially of hybrid crops.
- mutants selected under herbicide pressure vary according to the particular member of the herbicide family which is used. All of our mutants were selected under pressure of imazethapyr. Had a different imidazolinone herbicide been used, a different spectrum of mutants would have been selected.
- Figure 1 is a flow-chart showing the derivation of several generations of progeny from plants generated by this invention
- FIG. 2 shows the chemical structures of the herbicides used in this invention
- Figure 3 is a graph of ALS enzyme activity in the presence of imazethapyr.
- the enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 4 is a graph of ALS enzyme activity in the presence of imazapyr. The enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 5 is a graph of ALS enzyme activity in the presence of imazaquin. The enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 6 is a graph of ALS enzyme activity in the presence of chlorsulfuron. The enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 7 is a graph of ALS enzyme activity in the presence of chlorimuron.
- the enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 8 is a graph of ALS enzyme activity in the presence of thiacarburon.
- the enzyme is extracted from plants heterozygous for the resistance gene;
- Figure 9 is an enzyme activity graph of the activity of ALS extracted from leaves of progeny of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of imazethapyr (PURSUIT);
- Figure 10 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of imazaquin (SCEPTER);
- Figure 11 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of imazapyr (ARSENAL);
- Figure 12 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of chlorsulfuron (GLEAN);
- Figure 13 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of chlorimuron (CLASSIC);
- Figure 14 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of thiacarburon (HARMONY);
- Figure 15 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of a triazolopyrimidine;
- Figure 16 is an enzyme activity graph of the activity of ALS extracted from leaves of mutants 1 and 2 which are homozygous for the resistant allele and in the presence of a phenoxypyrimidine;
- Figure 17 is a dose response curve for the herbicide imazethapyr (PURSUIT);
- Figure 18 is a dose response curve for the herbicide imazaquin (SCEPTER).
- Figure 19 is a dose response curve for the herbicide chlorimuron (CLASSIC).
- Figure 20 is a dose response curve for the herbicide chlorsulfuron (GLEAN).
- Figure 21 is a dose response curve for a triazolopyrimidine herbicide
- Figure 22 is a dose response curve for a phenoxypyrimidine herbicide
- Figure 23 is a dose response curve for the herbicide imazethapyr (PURSUIT) for mutants 1 and 2 in both heterozygous and homozygous f-orms.
- EMS ethyl methane sulphonate
- Ml seed was sown, 100 seeds per tray, in WCB growing medium (low organic matter, 45% loam, 55% grit) and sprayed to 'run-off using a track sprayer, with a solution of imazethapyr (PURSUIT) at a concentration calculated to be the equivalent of 250 g/ha of active ingredient .
- the seeds were covered with 0.5 inch of WCB and grown in the glasshouse at 25°C.
- RFLP restriction fragment length polymorphism
- the M.BC- plants were self pollinated to give generation M.BC.S, which was again self-pollinated to give M-BC ⁇ S-. From that generation it was possible to identify, by the fact that the resistant trait is non-segregating, lines which are homozygous for the trait. These homozygous lines derived from Mutant No.l may be utilised for the production of Fl hybrids which possess resistance to the imidazolinone herbicides or for further breeding work.
- Seeds of generation M.BC., M-BC- of the nine mutants were sprayed pre-emergence with the herbicide imazethapyr (PURSUIT) (Trade Mark) at a rate of 250 g/ha and grown in a growth chamber (16 hour day, 27°C; 8 hour night, 17°C). Plants were harvested after 11 days.
- PURSUIT herbicide imazethapyr
- Tricine (Trade Mark), lOmM EDTA, 5mM pyruvate, 80 ⁇ M flavin adenine dinucleotide (FAD), ImM dithiothreitol (DTT), pH 8, plus 0.8g Polyclar AT.
- FAD flavin adenine dinucleotide
- DTT ImM dithiothreitol
- pH 8 plus 0.8g Polyclar AT.
- the homogenate was pressed through four layers of muslin and centrifuged at 30,000g for 20 minutes.
- the supernatant was brought to 65% saturation with ammonium sulphate, left to precipitate for 30 minutes and then centrifuged at 30.000g for 20 minutes.
- the pellet was resuspended in 2.5ml of 40mM Tricine, lOmM EDTA, 5mM pyruvate, 80 ⁇ M FAD, 25% (v/v) glycerol, ImM DTT, pH 8, and desalted into 3.5ml of 40mM Tricine, lOmM EDTA, 25% (v/v) glycerol, ImM DTT, pH 8.
- One hundred microlitres of the enzyme extract was used for each assay.
- Final reagent concentrations were: 120mM Tricine, 50mM pyruvate, lOmM MgCl 2/ 66mM FAD, 93//M thiamine pyrophosphate (TPP), pH 8, in the presence or absence of a herbicide of interest, in a volume of 750 ⁇ l .
- Incubations were carried out at 37°C for 30 minutes. Reactions were stopped with 250/1 of 1.84M sulphuric acid, and decarboxylation of the acetolactate carried out at 37°C for 75 minutes, or at 60°C for 30 minutes. Assay blanks had sulphuric acid added prior to the enzyme extract.
- the seeds used in this screen were populations of mixed tolerant and sensitive seed, segregating 1:1, derived from each of the heterogygous, tolerant mutants crossed with homozygous, sensitive UE95. All of the tolerant progeny are heterozygous for tolerance. Each of the mutants identified above were used except mutant 4.
- the controls were seed of self-pollinated UE95 which was pollinated in the same year as the tolerant lines.
- One litre of compost was placed in each seed tray of dimensions 19cm x 11cm x 5cm deep and firmed down flat. Two furrows 1 cm deep were drawn in the surface of the compost in each tray and six seeds were sown in each furrow (total twelve seeds per tray). Some trays were sown with 24 seeds, in which case three furrows were made and 8 seeds sown per furrow.
- each tray contained either the seed of one mutant or of the UE95 control for each herbicide application rate and for the untreated control.
- ALS-inhibitor herbicides Five different types were tested on the mutants plus imazethapyr (PURSUIT) as a comparison. Four rates of each were applied; approximately O.lx, 0.5x, lx and 4x the estimated field rates for each herbicide. The rates are the same for mutant 3 to 10 but higher rates for chlorimuron (CLASSIC) and lower rates for chlorsulfuron (GLEAN) were applied to mutants 1 and 2.
- herbicides with their estimated field application rates were used: chlorimuron (CLASSIC) 8 -13 g/Ha chlorsulfuron (GLEAN) 4 -26 g/HA imazaquin (SCEPTER) 100 -150 g/Ha triazolopyrimidine 10 - 30 g/Ha phenoxypyrimidine 100 - 200 g/Ha imazethapyr (PURSUIT) 70 - 140 g/Ha
- the rates of application used in the screening tests were as follows: chlorimuron (CLASSIC) 4,20,40,160 g/Ha (mutants 1 and 2); and, 8,40,80,320 g/Ha (mutants 3 to 10); chlorsulfuron (GLEAN) 5,25,50,200 g/Ha (mutants 1 and 2); and, 1,5,10,40 g/Ha (mutants 3 to 10); imazaquin (SCEPTER) 30,150,300,1200 g/Ha; triazolopyrimidine 5,25,50,200 g/Ha; phenoxypyrimidine 10,50,100,400 g/Ha; and, imazethapyr (PURSUIT) 30,150,300,1200 g/Ha
- the triazolopyrimidine and phenoxypyrimidine were formulated in an adjuvant wetting agent known as JF5969 and diluted with water to make a final concentration of 10% JF5969.
- a particular mutant may display relatively low resistance compared with the others against a particular herbicide this may make it eminently useful for providing plants which are intended to tolerate only small amounts of herbicide, for example, to resist a "carry-over" effect.
- the mutated lines of the present invention can be used in common with various second parent lines to produce herbicide resistant hybrids.
- Material from the homozygous lines may be entered into a breeding programme involving further outcrossing, selfing, visual selection and herbicide screening in order to produce a range of new herbicide-tolerant hybrid seed.
- the herbicide resistance trait can be transferred to new lines by the described mutation breeding approach or by conventional breeding practices, using selection for herbicide re ⁇ istantce as described hereinabove.
- Biochemical and molecular screeing techniques are also available to those skilled in the art to aid the process.
- the use of genetic engineering techniques are readily conceivable to isolate and transfer the resistance gene.
- the objective of a breeding preogramme may simply be the beneficial transfer of herbicide resistance or may be more complex, involving concurrent improvement of agronomic performance.
- a homologous maize gene probe was generated by PCR using data from the published literature (European Patent Application No. 257,993) which, it i ⁇ to be noted contains only a partial sequence.
- the probe sequence was cloned into plasmid pIEl03 using standard laboratory cloning procedures.
- Two cDNA libraries, both constructed in ⁇ -ZAP were screened with the insert from pIE103. These were (i) a pre-existing maize UE95 root cDNA library and (ii) a four-week old UE95 plant leaf cDNA library.
- R21 was fully sequenced by "oligo walking” u ⁇ ing plasmid sequencing. The complete (>2kb) DNA sequence wa ⁇ thereby obtained. 15. Screening of the leaf library and sequencing of L3
- L2 wa ⁇ found to be truncated, L3 wa ⁇ chosen as a representative of a UE95 wild type class 2 ALS gene for sequencing.
- the entire gene can be spanned by fourteen sequencing primers although some further sequencing was required to resolve ambiguities, particularly in the GC-rich
- Mutant 1 - clone lb
- R21 wild-type gene
- Sequence of Mutant 1 A single base mutation was detected towards the 5' end of the coding region (indicated by an asteri ⁇ k in Figure 24). Thi ⁇ mutation, of guanine to adenine tran ⁇ ition, re ⁇ ult ⁇ in an alanine to threonine ⁇ ubstitution. Such a mutation has been previously reported a ⁇ conferring re ⁇ i ⁇ tance to the sulphonylurea herbicides but our mutant 1 po ⁇ e ⁇ es no such resi ⁇ tance. 19. Sequence of Mutant 2
- a guanine to adenine transition 52 bases from the 3' end of the coding region (indicated by an asteri ⁇ k in Figure 25) re ⁇ ult ⁇ in a ⁇ erine to a ⁇ paragine ⁇ ub ⁇ titution in the amino acid ⁇ equence.
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Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU88681/91A AU663856B2 (en) | 1990-11-14 | 1991-11-08 | Herbicide resistant plants |
JP3518003A JPH06502074A (ja) | 1990-11-14 | 1991-11-08 | 除草剤抵抗性トウモロコシ |
BR919107080A BR9107080A (pt) | 1990-11-14 | 1991-11-08 | Dna codificando uma sintase de acetolactato de mutante,dna de planta,planta sementes de progenia de planta |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB909024728A GB9024728D0 (en) | 1990-11-14 | 1990-11-14 | Herbicide resistant plants |
GB9024728.9 | 1990-11-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1992008794A1 true WO1992008794A1 (fr) | 1992-05-29 |
Family
ID=10685344
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1991/001972 WO1992008794A1 (fr) | 1990-11-14 | 1991-11-08 | Plantes resistantes aux herbicides |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0557352A1 (fr) |
JP (1) | JPH06502074A (fr) |
AU (1) | AU663856B2 (fr) |
BR (1) | BR9107080A (fr) |
CA (1) | CA2096348A1 (fr) |
GB (1) | GB9024728D0 (fr) |
WO (1) | WO1992008794A1 (fr) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0525384A2 (fr) * | 1991-07-31 | 1993-02-03 | American Cyanamid Company | Mutants de AHAS résistants aux imidazolinones |
WO1996007746A1 (fr) * | 1994-09-08 | 1996-03-14 | American Cyanamid Company | Promoteur de l'acetohydroxyacide-synthase servant a l'expression de genes introduits dans des vegetaux |
EP0716147A2 (fr) | 1994-11-09 | 1996-06-12 | Nippon Paper Industries Co., Ltd. | Procédé de préparation des plantes transgéniques |
US6274796B1 (en) | 1996-04-29 | 2001-08-14 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
EP1183346A1 (fr) * | 1999-05-24 | 2002-03-06 | New England Biolabs, Inc. | Procede de generation de genes non transferables separes capables d'exprimer un produit proteique actif |
US6653529B2 (en) * | 2000-04-28 | 2003-11-25 | Basf Aktiengesellschaft | Use of the maize X112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides |
US6943280B2 (en) | 2000-05-10 | 2005-09-13 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetohydroxycid synthase-inhibiting herbicides |
US7019196B1 (en) | 1998-11-05 | 2006-03-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US20070033670A1 (en) * | 2003-05-28 | 2007-02-08 | Basf Aktiengesellschaft | Wheat plants having increased tolerance to imidazolinone herbicides |
US9035133B2 (en) | 2006-12-12 | 2015-05-19 | Basf Agrochemical Products B.V. | Herbicide-resistant sunflower plants and methods of use |
US20150289468A1 (en) * | 2002-07-10 | 2015-10-15 | The Department Of Agriculture, Western Australia | Wheat plants having increased resistance to imidazolinone herbicides |
US10017827B2 (en) | 2007-04-04 | 2018-07-10 | Nidera S.A. | Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0461355T3 (da) * | 1990-05-09 | 1997-08-25 | American Cyanamid Co | Fremgangsmåde til at hindre afgrødeskade i nærvær af synergistiske pesticidkombinationer |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0257993A2 (fr) * | 1986-08-26 | 1988-03-02 | E.I. Du Pont De Nemours And Company | Fragment d'acide nucléique codant la synthase acétolactate végétale résistante aux herbicides |
EP0360750A2 (fr) * | 1988-09-22 | 1990-03-28 | Ciba-Geigy Ag | Plantes tolérantes aux herbicides |
WO1990014000A1 (fr) * | 1989-05-17 | 1990-11-29 | Imperial Chemical Industries Plc | Mais resistant aux herbicides |
EP0154204B1 (fr) * | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Résistance aux herbicides dans des plantes |
-
1990
- 1990-11-14 GB GB909024728A patent/GB9024728D0/en active Pending
-
1991
- 1991-11-08 WO PCT/GB1991/001972 patent/WO1992008794A1/fr not_active Application Discontinuation
- 1991-11-08 AU AU88681/91A patent/AU663856B2/en not_active Ceased
- 1991-11-08 EP EP19910919915 patent/EP0557352A1/fr not_active Withdrawn
- 1991-11-08 JP JP3518003A patent/JPH06502074A/ja active Pending
- 1991-11-08 CA CA 2096348 patent/CA2096348A1/fr not_active Abandoned
- 1991-11-08 BR BR919107080A patent/BR9107080A/pt not_active Application Discontinuation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0154204B1 (fr) * | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Résistance aux herbicides dans des plantes |
EP0257993A2 (fr) * | 1986-08-26 | 1988-03-02 | E.I. Du Pont De Nemours And Company | Fragment d'acide nucléique codant la synthase acétolactate végétale résistante aux herbicides |
EP0360750A2 (fr) * | 1988-09-22 | 1990-03-28 | Ciba-Geigy Ag | Plantes tolérantes aux herbicides |
WO1990014000A1 (fr) * | 1989-05-17 | 1990-11-29 | Imperial Chemical Industries Plc | Mais resistant aux herbicides |
Non-Patent Citations (4)
Title |
---|
CHEMICAL ABSTRACTS, Vol. 112, 1990, Columbus, Ohio, US., ANDERSON P.C. et al., "Herbicide-tolerant Mutants of Corn", page 259, Abstract No. 193711; & GENOME, Vol. 31, No. 2, 1989, pages 994-999. * |
NUCLEIC ACIDS RESEARCH, Vol. 18, No. 8, 25 April 1990, Arlington, Virginia, US., SATHASIVAN K. et al., "Nucleotide Sequence of a Mutant Acetolactate Synthase Gene from an Imidazolinone-resistant Arabidopsis Thaliana Var. Columbia", page 2188. * |
PLANT PHYSIOLOGY, Vol. 92, No. 4, April 1990, Rockville, MD, USA., HAUGHN G.W. et al., "A Mutation Causing Imidazolinone Resistance Maps to the Csr1 Locus of Arabidopsis Thalina", pages 1081-1085. * |
PLANT PHYSIOLOGY. SUPPLEMENT, Vol. 93, No. 1, May 1990, Rockville, MD, USA., SATHASIVAN K. et al., "Isolation and Characterization of a Mutant Acetolactate Synthase Gene from an Imidazolinone-resistant Arabidopsis Thaliana Var. Columbia", page 157, Abstract 917. * |
Cited By (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0525384A2 (fr) * | 1991-07-31 | 1993-02-03 | American Cyanamid Company | Mutants de AHAS résistants aux imidazolinones |
EP0525384A3 (en) * | 1991-07-31 | 1993-06-23 | American Cyanamid Company | Imidazolinone resistant ahas mutants |
US6444875B1 (en) | 1991-07-31 | 2002-09-03 | Basf Aktiengesellschaft | Imidazolinone resistant AHAS mutants |
WO1996007746A1 (fr) * | 1994-09-08 | 1996-03-14 | American Cyanamid Company | Promoteur de l'acetohydroxyacide-synthase servant a l'expression de genes introduits dans des vegetaux |
MD2312B2 (ro) * | 1994-09-08 | 2003-11-30 | Эмерикэн Сайенэмид Компэни | Succesiune nucleotidă izolată a promotorului genei sintazei acetohidroxiacizilor; vector; procedeu de expresie a genei heteroloage; construct de acid nucleic şi metodă de selecţie a materialului vegetal transgenic |
EP0716147A2 (fr) | 1994-11-09 | 1996-06-12 | Nippon Paper Industries Co., Ltd. | Procédé de préparation des plantes transgéniques |
US6274796B1 (en) | 1996-04-29 | 2001-08-14 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US9029642B2 (en) * | 1998-11-05 | 2015-05-12 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US7754947B2 (en) | 1998-11-05 | 2010-07-13 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US7495153B2 (en) | 1998-11-05 | 2009-02-24 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US7345221B2 (en) | 1998-11-05 | 2008-03-18 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US7019196B1 (en) | 1998-11-05 | 2006-03-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
EP1183346A1 (fr) * | 1999-05-24 | 2002-03-06 | New England Biolabs, Inc. | Procede de generation de genes non transferables separes capables d'exprimer un produit proteique actif |
EP1183346A4 (fr) * | 1999-05-24 | 2004-08-18 | New England Biolabs Inc | Procede de generation de genes non transferables separes capables d'exprimer un produit proteique actif |
US6653529B2 (en) * | 2000-04-28 | 2003-11-25 | Basf Aktiengesellschaft | Use of the maize X112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides |
US6943280B2 (en) | 2000-05-10 | 2005-09-13 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetohydroxycid synthase-inhibiting herbicides |
US7399905B2 (en) | 2000-05-10 | 2008-07-15 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetohydroxyacid synthase-inhibiting herbicides in rice |
US9090904B2 (en) | 2000-05-10 | 2015-07-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetohydroxyacid synthase-inhibiting herbicides |
US20150289468A1 (en) * | 2002-07-10 | 2015-10-15 | The Department Of Agriculture, Western Australia | Wheat plants having increased resistance to imidazolinone herbicides |
US10575485B2 (en) * | 2002-07-10 | 2020-03-03 | Western Australian Agriculture Authority | Wheat plants having increased resistance to imidazolinone herbicides |
US20070033670A1 (en) * | 2003-05-28 | 2007-02-08 | Basf Aktiengesellschaft | Wheat plants having increased tolerance to imidazolinone herbicides |
US9382526B2 (en) * | 2003-05-28 | 2016-07-05 | Basf Aktiengesellschaft | Wheat plants having increased tolerance to imidazolinone herbicides |
US9035133B2 (en) | 2006-12-12 | 2015-05-19 | Basf Agrochemical Products B.V. | Herbicide-resistant sunflower plants and methods of use |
US10017827B2 (en) | 2007-04-04 | 2018-07-10 | Nidera S.A. | Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use |
Also Published As
Publication number | Publication date |
---|---|
JPH06502074A (ja) | 1994-03-10 |
AU663856B2 (en) | 1995-10-26 |
EP0557352A1 (fr) | 1993-09-01 |
GB9024728D0 (en) | 1991-01-02 |
CA2096348A1 (fr) | 1992-05-15 |
AU8868191A (en) | 1992-06-11 |
BR9107080A (pt) | 1993-09-21 |
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