WO1991013141A1 - Lipase containing liquid pre-spotter and use of such pre-spotter - Google Patents
Lipase containing liquid pre-spotter and use of such pre-spotter Download PDFInfo
- Publication number
- WO1991013141A1 WO1991013141A1 PCT/DK1991/000058 DK9100058W WO9113141A1 WO 1991013141 A1 WO1991013141 A1 WO 1991013141A1 DK 9100058 W DK9100058 W DK 9100058W WO 9113141 A1 WO9113141 A1 WO 9113141A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- spotter
- lipase
- fat
- alcohol
- laundry
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
Definitions
- the invention relates to a lipase containing liquid pre-spotter and to a use of such pre-spotter.
- the purpose of the invention is the provision of a simple lipase containing liquid pre-spotter, which exhibits an improved fat and oil removing effect and an improved lipase stability.
- the lipase containing and surfactant free liquid pre-spotter according to the invention comprises a liquid mixture of an alcohol and water, with an alcohol content above 20% by weight, and an effective amount of lipase, whereby tne balance is water.
- the pre-spotter according to the invention does not contain any surfactant, and thus exhibits a simple composition, in dischargee of the fact that the pre-spotter according to the invention is very effective, as demonstrated in the following examples.
- alcohol comprises both monovalent alcohols like ethanol and polyvalent alcohols (polyois) like propylene glycol. If the alcohol content is below 10% by weight, the effect and the stability of the lipase tend to be unsatisfactory.
- an effective amount of lipase means an amount, which is able to remove fat and oil stains effectively, when evaluated by swatch tests described later in this specification. It is intended that all lipases can be used in the pre-spotter according to the invention. Reference can be made to Research
- the pre-spotter further comprises a lipase stabilizer.
- a lipase stabilizer examples include CaCl2 (e.g. 0.1-0.5%) or a protease inhibitor (such as borate and formate).
- the alcohol is a mono- or polyvalent alcohol with 2-3 carbon atoms, preferably ethanol, propylene glycol, glycerol or a mixture of two or more of these.
- the lipase activity is at least 10,000 LU/liter of liquid pre-spotter. It has been found that the time interval between application of pre-spotter and main wash is undue long in order to obtain an efficient fat and oil removal, if the lipase activity is less than 10,000 LU/liter of liquid pre-spotter. On the other hand, for economic reasons a suitable upper limit for the lipase activity is 5,000,000 LU/liter of liquid pre-spotter.
- the lipase is Upolase*. It has been found that the fat and oil removal is very efficient in this embodiment.
- the pre-spotter contains an antimicrobial agent.
- examples of such agents are inorganic salts (such as NaCI), sugars (such as sucrose and glucose), organic acids (such as benzoic, sorbic, propionic, lactic and formic acids) which are generally effective in amounts of 0.01-2% at low pH (below 5).
- Other examples of stabilizing agents are antioxidants (such as sulphur dioxide), 1 ,2-benz-iso- thioazolin-3-one (BIT) and parabens. Some of these may also serve to improve enzyme stability.
- the pH value is between 4 and 10, preferably between 5 and 9.
- the stability optimum and the activity optimum of the lipase should be taken into consideration.
- the invention comprises a use of the pre-spotter according to the invention, and this use is characterized by the fact that the pre-spotter is applied locally to the fat or oil spots on the laundry, that the laundry is left alone for a certain time period, and that the laundry subsequently is exposed to a low temperature washing process.
- the certain time period alluded to above is normally at least 5 minutes, but it can be shorter in case the lipase activity in the pre-spotter is extraordinarily high.
- ester formed by the above indicated ester synthesis performs as a surfactant, improving the fat and oil removing effect.
- part of the improved fat and oil removing effect of the pre-spotter according to the invention has to be ascribed to this ester synthesis; however, applicant does not want to be bound to this hypothesis.
- the lipase activity is measured in LU lipase activity units.
- the LU activity determination method is based on hydrolysis of tributyrin in a pH-stat.
- 1 LU lipase Unit
- 1 LU lipase Unit is the amount of enzyme which liberates 1 ⁇ mole titratable butyric acid per minute at 30" C and pH 7.0 with gum arabic as an emulsifier. Further details are given in Novo Nordisk analytical Method AF 95/5, available on request from Novo Nordisk A/S, Novo Alle, DK-2880 Bagsvaerd, Denmark.
- Embodiments of liquid pre-spotter is applied to the fat stain on a test swatch by means of a pipette.
- the amount of pre-spotter used depends on the size of the stain and on the kind of fabric; typically 0.5-3 ml of pre-spotter is satisfactory.
- After 5-30 minutes of incubation the swatches are washed in a Laboratory washing machine (Launder-o-meter or Terg-o-tometer) at low temperature (20-40 °C).
- the detergent used in the washing process is a standarc powder or liquid detergent.
- Swatches The swatches are . made from coloured (e.g. blue or green) cotton fabric.
- the fabric is cut into test pieces with the dimensions 12x12 cm, and 75- 150 ⁇ of fatty material (e.g. lard, margarine, butter, or olive oil) is applied to the center of each swatch.
- the amount of fatty material applied depends on the type of fatty material used.
- the fatty material is heated to approx. 70 °C before application in order to facilitate the penetration of fatty material into the fabric.
- the stained fabric was aged at room temperature for 1 day before treatment. Evaluation: After washing and drying the swatches are gently ironed.
- the swatches are visually inspected and compared to reference swatches which have been identically treated but without any lipase added to the pre-spotter formulation.
- extraction of the residual fatty material can be carried out (Soxhlet, chloroform, 5 hours).
- Stain Lard on green cotton sheet. 75 ⁇ of lard was applied on each swatch. After stain application, the swatches were heat treated in an oven for 30 minutes at 75 °C in order to obtain a better distribution of the stain.
- Pre- spotting 1 ml of pre-spotter is applied with a pipette on the centre of the stains and subsequently the swatches are incubated for 15 minutes at roorr temperature.
- the pre-spotter formulations contained 1 % Lipolase* 100L (100,000
- the lipase activity in the pre-spotters was 1000 LU/g.
- the same pre-spotter formulations, but without added lipase were evaluated as well. Washing: Immediately after pre-spotting the swatches were washed in a Terg- o-tometer (40 °C, 20 minutes, 18° dH, pH 9.5) with a simplifiec
- European powder detergent containing 1.75 g/l of sodium triphosphate, 0.40 g/l of sodium metasilicate, 2.00 g/l of sodium sulphate, 0.30 g/l of Berol 160 (alcohol ethoxylate from Beroi Kemi AB.
- Nansa S80/S LAS from Albright & Wilson
- Example 5 evaluation method as described in Example 1 was used; the only difference being that the dosage of Lipolase* 100 L was varied between 0 and 5%.
- the pre-spotter formulation contained 5% (w/w) Lipolase* 100 L, 45%
- Lipolase* The enzymatic stability of Lipolase* was investigated in 5 different pre- spotter formulations.
- the pre-spotter formulations were stored at 30 °C in closed bottles and the activity was followed over a 2 months period.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Detergent Compositions (AREA)
Abstract
The pre-spotter comprises a surfactant free liquid pre-spotter containing alcohol, water and an effective amount of lipase. This pre-spotter is simple and it exhibits an improved fat and oil removing effect and an improved lipase stability.
Description
UPASE CONTAINING UQUID PRE-SPOTTER AND USE OF SUCH PRE-SPOTTER
The invention relates to a lipase containing liquid pre-spotter and to a use of such pre-spotter.
In relation to washing processes it is described that fat or oil stains, which are difficult to remove, can be treated with a lipase containing liquid pre- spotter, which is applied directly to the stain before the washing process. However, to the best of our knowledge no lipase containing pre-spotter is commercialized, probably due to lipase stability or performance problems. It is further described that the thus treated laundry is subsequently washed, usually at relatively low temperature, normally after a short period of time. It is one of the advantages by using a lipase containing pre-spotter that the subsequent washing process can be carried out at relatively low temperature, i.e. below around 60 °C. If no lipase containing pre-spotter is used, usually the washing process will have to be carried out at around 100βC and at high alkalinity in order to have the fat or oil stains removed effectively, but even under these conditions, i.e. at around 100°C and at high alkalinity, some obstinate fat or oil stains cannot be removed. Reference can be made to EP 177 183, which describes special lipase containing pre-spotters in which the enzymes are encapsulated in reversed micelles. It has been found tnε: the fat and oil removing effect of the prior art lipase containing pre-spotters and the lipase stability in the prior art lipase containing pre-spotters are open to improvement.
Thus the purpose of the invention is the provision of a simple lipase containing liquid pre-spotter, which exhibits an improved fat and oil removing effect and an improved lipase stability. The lipase containing and surfactant free liquid pre-spotter according to the invention comprises a liquid mixture of an alcohol and water, with an alcohol content above 20% by weight, and an effective amount of lipase, whereby tne balance is water. It should be noted that the pre-spotter according to the invention does not contain any surfactant, and thus exhibits a simple composition, in soite
of the fact that the pre-spotter according to the invention is very effective, as demonstrated in the following examples.
In this specification with claims the term alcohol comprises both monovalent alcohols like ethanol and polyvalent alcohols (polyois) like propylene glycol. If the alcohol content is below 10% by weight, the effect and the stability of the lipase tend to be unsatisfactory.
The term "an effective amount of lipase" means an amount, which is able to remove fat and oil stains effectively, when evaluated by swatch tests described later in this specification. It is intended that all lipases can be used in the pre-spotter according to the invention. Reference can be made to Research
Disclosure June 1988, No. 290, 29056.
In a preferred embodiment of the pre-spotter according to the invention the pre-spotter further comprises a lipase stabilizer. Examples of suitable lipase stabilizers are CaCl2 (e.g. 0.1-0.5%) or a protease inhibitor (such as borate and formate).
In a preferred embodiment of the pre-spotter according to the invention the alcohol is a mono- or polyvalent alcohol with 2-3 carbon atoms, preferably ethanol, propylene glycol, glycerol or a mixture of two or more of these.
It has been found that the fat and oil removing effect is most satisfactory with these alcohols.
In a preferred embodiment of the pre-spotter according to the invention the lipase activity is at least 10,000 LU/liter of liquid pre-spotter. It has been found that the time interval between application of pre-spotter and main wash is undue long in order to obtain an efficient fat and oil removal, if the lipase activity is less than 10,000 LU/liter of liquid pre-spotter. On the other hand, for economic reasons a suitable upper limit for the lipase activity is 5,000,000 LU/liter of liquid pre-spotter.
In a preferred embodiment of the pre-spotter according to the invention the lipase is Upolase*. It has been found that the fat and oil removal is very efficient in this embodiment.
In a preferred embodiment of the pre-spotter according to the invention the pre-spotter contains an antimicrobial agent. Examples of such agents are inorganic salts (such as NaCI), sugars (such as sucrose and glucose), organic acids (such as benzoic, sorbic, propionic, lactic and formic acids) which are generally effective in amounts of 0.01-2% at low pH (below 5). Other examples of stabilizing agents are antioxidants (such as sulphur dioxide), 1 ,2-benz-iso- thioazolin-3-one (BIT) and parabens. Some of these may also serve to improve enzyme stability.
In a preferred embodiment of the pre-spotter according to the invention the pH value is between 4 and 10, preferably between 5 and 9. in selecting the pH value for a specific pre-spotter, the stability optimum and the activity optimum of the lipase should be taken into consideration.
Also, the invention comprises a use of the pre-spotter according to the invention, and this use is characterized by the fact that the pre-spotter is applied locally to the fat or oil spots on the laundry, that the laundry is left alone for a certain time period, and that the laundry subsequently is exposed to a low temperature washing process.
The certain time period alluded to above is normally at least 5 minutes, but it can be shorter in case the lipase activity in the pre-spotter is extraordinarily high.
From the brochure Novo Detergent Enzymes B 435b-GB July 1989 it appears that very difficult stains can be removed by introduction of a pre-spotter with a Lipolase® containing detergent in a specific test, which utilized a heavy duty liquid detergent. This heavy duty liquid detergent contained around 28% surfactant. It has been found that during the pre-spotting not exclusively a fat hydrolysis takes place but additionally an ester synthesis based on the alcohol in the pre-spotter and the fatty acids liberated from the fat or oil stains by means of the iipolytically catalyzed hydrolysis. It is surprising that an ester synthesis takes place because of the high water activity. Aiso, the ester formed by the above indicated ester synthesis performs as a surfactant, improving the fat and oil
removing effect. Thus, it is assumed that part of the improved fat and oil removing effect of the pre-spotter according to the invention has to be ascribed to this ester synthesis; however, applicant does not want to be bound to this hypothesis.
In the examples shown below the lipase activity is measured in LU lipase activity units. The LU activity determination method is based on hydrolysis of tributyrin in a pH-stat. 1 LU (lipase Unit) is the amount of enzyme which liberates 1 μmole titratable butyric acid per minute at 30" C and pH 7.0 with gum arabic as an emulsifier. Further details are given in Novo Nordisk analytical Method AF 95/5, available on request from Novo Nordisk A/S, Novo Alle, DK-2880 Bagsvaerd, Denmark.
In the examples shown below the following general procedure was used.
Method: Embodiments of liquid pre-spotter is applied to the fat stain on a test swatch by means of a pipette. The amount of pre-spotter used depends on the size of the stain and on the kind of fabric; typically 0.5-3 ml of pre-spotter is satisfactory. After 5-30 minutes of incubation the swatches are washed in a Laboratory washing machine (Launder-o-meter or Terg-o-tometer) at low temperature (20-40 °C). The detergent used in the washing process is a standarc powder or liquid detergent.
Swatches: The swatches are . made from coloured (e.g. blue or green) cotton fabric. The fabric is cut into test pieces with the dimensions 12x12 cm, and 75- 150 μ of fatty material (e.g. lard, margarine, butter, or olive oil) is applied to the center of each swatch. The amount of fatty material applied depends on the type of fatty material used. The fatty material is heated to approx. 70 °C before application in order to facilitate the penetration of fatty material into the fabric. The stained fabric was aged at room temperature for 1 day before treatment.
Evaluation: After washing and drying the swatches are gently ironed. The swatches are visually inspected and compared to reference swatches which have been identically treated but without any lipase added to the pre-spotter formulation. In order to quantify the efficiency of the lipase, extraction of the residual fatty material can be carried out (Soxhlet, chloroform, 5 hours).
EXAMPLE 1
Effect of Lipolase* in pre-spotter formulations based on water and propylene glycol
Stain: Lard on green cotton sheet. 75 μ\ of lard was applied on each swatch. After stain application, the swatches were heat treated in an oven for 30 minutes at 75 °C in order to obtain a better distribution of the stain.
Pre- spotting: 1 ml of pre-spotter is applied with a pipette on the centre of the stains and subsequently the swatches are incubated for 15 minutes at roorr temperature.
The pre-spotter formulations contained 1 % Lipolase* 100L (100,000
LU/g) and 99% (w/w) of a mixture of water and propylene glycol.
Accordingly the lipase activity in the pre-spotters was 1000 LU/g. For comparison, the same pre-spotter formulations, but without added lipase, were evaluated as well. Washing: Immediately after pre-spotting the swatches were washed in a Terg- o-tometer (40 °C, 20 minutes, 18° dH, pH 9.5) with a simplifiec
European powder detergent containing 1.75 g/l of sodium triphosphate, 0.40 g/l of sodium metasilicate, 2.00 g/l of sodium sulphate, 0.30 g/l of Berol 160 (alcohol ethoxylate from Beroi Kemi AB.
Sweden), and 0.50 g/l of Nansa S80/S (LAS from Albright & Wilson,
UK).
Evalua¬ tion: A trained test panel (13 persons) evaluated the stain removal by visual inspection.
Def.
Effect of lipase dosage in a pre-spotter formulation based on water and propylene αlvcol
The same kind of test material and the same pre-spotting, washing,
5 and evaluation method as described in Example 1 was used; the only difference being that the dosage of Lipolase* 100 L was varied between 0 and 5%.
Results (mean score of test panel evaluation):
EXAMPLE 3
Effect of different lipases in pre-spotter formulations based on water and propylene 0 αlvcol
The same experiments as described in Example 2 were carried out, the only difference being that four lipases of different origin (2 fungal, 1 bacterial and 1 from yeast) were investigated. The lipases were compared on an LU-activity basis. Results (mean score of test panel evaluation):
1) see Huge-Jensen et al. (1989), Lipids 24, 781-785
2) see EP 0214761 3) Sigma L1754
EXAMPLE 4
Effect of Lipolase* in pre-spotter formulations containing different alcohols
The same kind of test material and the same pre-spotting washing, and evaluation method as described in Example 1 was used. Results (mean score of test panel evaluation):
EXAMPLE 5
5 Effect of Lipolase* in water/propylene glycol formulation
Stain: 3 different stains were investigated
1) Lipstick on white cotton (interlock type). The lipstick was applied directly onto the fabric in a homogenous layer (diameter of the spot: 0 40 mm)
2) Lard on purple cotton (interlock type). 150 μ\ of lard per swatch.
3) Olive oil on dark green cotton (interlock type). 150 μl of olive oil per swatch.
Pre- spotting: 3 ml of pre-spotter is applied to the centre of the stains and subsequently the swatches are incubated for 15 minutes at room temperature.
The pre-spotter formulation contained 5% (w/w) Lipolase* 100 L, 45%
(w/w) water, and 50% (w/w) propylene glycol. Accordingly the lipase activity in the pre-spotter was 5000 LU/g.
For comparison, the same pre-spotter formulation, but without added lipase, i.e. 50% w/w water and 50% w/w propylene glycol, were evaluated as well.
Washing: Immediately after pre-spotting the swatches were washed in a Terg- o-tometer (30 βC, 30 minutes, 18° dH, pH 9.5) with a commercial
European powder detergent (5 g/l).
Evalua- tion: The performance of the pre-spotter was evaluated by the same test panel method as described in Example 1.
Results: The mean scores (12 persons) were as follows:
EXAMPLE 6
Storage stability of pre-spotter formulations
The pre-spotter formulations were stored at 30 °C in closed bottles and the activity was followed over a 2 months period.
EXAMPLE 7
Synthesis of esters vs hydrolysis under pre-soottinα conditions
In order to investigate lipase catalyzed formation of esters vs hydrolysis the following experiment was carried out. Olive oil adsorbed to PVC particles was incubated in pre-spotting formulations containing a lipase. After 5 minutes of incubation the reaction was stopped and fatty matter was extracted and analyzed
by chromatography. Conditions of incubation: 30 'C, pH 7.0, 0.33 g olive oil adsorbed on 1.67 g PVC powder, 20 g pre-spotter, 1500 LU. Results:
Claims
1. Lipase containing and surfactant free liquid pre-spotter comprising a liquid mixture of an alcohol and water, with an alcohol content above 20% by weight, and an effective amount of lipase, whereby the balance is water.
2. Pre-spotter according to Claim 1, which further comprises a lipase stabilizer.
3. Pre-spotter according to Claims 1 to 2, in which the alcohol is ethanol, propylene glycol, glycerol or a mixture of two or more of these.
4. Pre-spotter according to Claims 1 to 3, in which the iipase activity is at least 10,000 LU/liter of liquid pre-spotter.
5. Pre-spotter according to Claims 1 to 4, wherein the lipase is Lipolase®.
6. Pre-spotter according to Claims 1 to 5, wherein the pre-spotter contains an antimicrobial agent.
7. Pre-spotter according to Claims 1 to 6, wherein the pH value is between 4 and 10, preferably between 5 and 9.
8. Use of the pre-spotter according to Claims 1 -7, characterized by the fact that the pre-spotter is applied locally to the fat or oil spots on the laundry, that the laundry is left alone for a certain time period, and that the laundry subsequently is exposed to a low temperature washing process.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT91905035T ATE101642T1 (en) | 1990-03-01 | 1991-02-28 | LIPASE-CONTAINING LIQUID SOAKING AGENT AND USE THEREOF. |
DE69101219T DE69101219T2 (en) | 1990-03-01 | 1991-02-28 | LIPASE CONTAINING LIQUID SOAKING AGENT AND ITS USE. |
US07/867,663 US5447649A (en) | 1990-03-01 | 1992-02-28 | Lipase containing liquid pre-spotter and use of such pre-spotter |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK53090A DK53090D0 (en) | 1990-03-01 | 1990-03-01 | |
DK77290A DK77290D0 (en) | 1990-03-01 | 1990-03-26 | LIPASE-CONTAINING LIQUID PRE-SPOTTER AND USING SUCH PRE-SPOTTER |
DK772/90 | 1991-01-24 | ||
DK530/90 | 1991-01-24 | ||
DK11891A DK11891D0 (en) | 1991-01-24 | 1991-01-24 | LIPASE CONTAINING LIQUID PRE-SPOTTER AND USING SUCH PRE-SPOTTER. |
DK118/91 | 1991-01-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1991013141A1 true WO1991013141A1 (en) | 1991-09-05 |
Family
ID=27220418
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1991/000058 WO1991013141A1 (en) | 1990-03-01 | 1991-02-28 | Lipase containing liquid pre-spotter and use of such pre-spotter |
PCT/DK1991/000059 WO1991013142A1 (en) | 1990-03-01 | 1991-02-28 | Lipase containing liquid pre-spotter and use of such pre-spotter |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1991/000059 WO1991013142A1 (en) | 1990-03-01 | 1991-02-28 | Lipase containing liquid pre-spotter and use of such pre-spotter |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0517761B1 (en) |
JP (1) | JP2930719B2 (en) |
DE (1) | DE69101219T2 (en) |
DK (1) | DK0517761T3 (en) |
ES (1) | ES2051122T3 (en) |
WO (2) | WO1991013141A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5510052A (en) * | 1994-08-25 | 1996-04-23 | Colgate-Palmolive Co. | Enzymatic aqueous pretreatment composition for dishware |
EP1029972A1 (en) * | 1991-12-20 | 2000-08-23 | Novo Nordisk A/S | Removal of hydrophobic esters from textiles |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605881A (en) * | 1993-09-03 | 1997-02-25 | Minolta Co., Ltd. | Cleaning liquid for recycling copy medium for electrophotography |
CA2161975C (en) * | 1994-03-21 | 2000-02-01 | Jeanne A. O'brien | Stable enzyme-containing aqueous laundry prespotting composition |
WO2014200657A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces xiamenensis |
WO2014200658A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from promicromonospora vindobonensis |
WO2014200656A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces umbrinus |
WO2014204596A1 (en) | 2013-06-17 | 2014-12-24 | Danisco Us Inc. | Alpha-amylase from bacillaceae family member |
WO2015050724A1 (en) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof |
WO2015050723A1 (en) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
US20160272957A1 (en) | 2013-11-20 | 2016-09-22 | Danisco Us Inc. | Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof |
WO2017173324A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
WO2017173190A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
WO2018050047A1 (en) * | 2016-09-13 | 2018-03-22 | Novozymes A/S | Detergent composition, use of detergent composition and method for laundering textile |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3003766A1 (en) * | 1979-02-07 | 1980-08-21 | Unilever Nv | DETERGENT FOR PRE-TREATING LAUNDRY |
DE3223568A1 (en) * | 1982-06-24 | 1983-12-29 | Peter 5275 Bergneustadt Penz jun. | Stain-removing composition and method for removing stains |
EP0383373A2 (en) * | 1989-02-03 | 1990-08-22 | Sara Lee/DE N.V. | A detergent composition in liquid form for the pretreatment of textiles |
-
1991
- 1991-02-28 WO PCT/DK1991/000058 patent/WO1991013141A1/en active IP Right Grant
- 1991-02-28 JP JP3504594A patent/JP2930719B2/en not_active Expired - Lifetime
- 1991-02-28 EP EP19910905035 patent/EP0517761B1/en not_active Expired - Lifetime
- 1991-02-28 DE DE69101219T patent/DE69101219T2/en not_active Expired - Fee Related
- 1991-02-28 ES ES91905035T patent/ES2051122T3/en not_active Expired - Lifetime
- 1991-02-28 DK DK91905035T patent/DK0517761T3/en active
- 1991-02-28 WO PCT/DK1991/000059 patent/WO1991013142A1/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3003766A1 (en) * | 1979-02-07 | 1980-08-21 | Unilever Nv | DETERGENT FOR PRE-TREATING LAUNDRY |
DE3223568A1 (en) * | 1982-06-24 | 1983-12-29 | Peter 5275 Bergneustadt Penz jun. | Stain-removing composition and method for removing stains |
EP0383373A2 (en) * | 1989-02-03 | 1990-08-22 | Sara Lee/DE N.V. | A detergent composition in liquid form for the pretreatment of textiles |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1029972A1 (en) * | 1991-12-20 | 2000-08-23 | Novo Nordisk A/S | Removal of hydrophobic esters from textiles |
US5510052A (en) * | 1994-08-25 | 1996-04-23 | Colgate-Palmolive Co. | Enzymatic aqueous pretreatment composition for dishware |
Also Published As
Publication number | Publication date |
---|---|
DE69101219D1 (en) | 1994-03-24 |
EP0517761B1 (en) | 1994-02-16 |
WO1991013142A1 (en) | 1991-09-05 |
ES2051122T3 (en) | 1994-06-01 |
JPH05504368A (en) | 1993-07-08 |
DE69101219T2 (en) | 1994-06-01 |
JP2930719B2 (en) | 1999-08-03 |
EP0517761A1 (en) | 1992-12-16 |
DK0517761T3 (en) | 1994-05-09 |
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