WO1991011507A1

WO1991011507A1 - Indication and test device for determining concentrations of substrates of fermentation reactions

Info

Publication number: WO1991011507A1
Application number: PCT/SU1990/000028
Authority: WO
Inventors: Yakov Alexandrovich Alexandrovsky
Original assignee: Institut Khimicheskoi Fiziki Imeni N.N.Semenova Akademii Nauk Sssr
Priority date: 1990-01-30
Filing date: 1990-01-30
Publication date: 1991-08-08

Abstract

An indication and test device for determining the concentration of substrates of fermentation reactions comprises a dismountable capsule (1) made of a heat-resistant transparent material and having a main (2) and an additional (3) part mutually separated but forming a closed chamber at the moment of interaction. In the main part (2) of the capsule (1) is located a layer (4) for placing a sample (8) to be tested, and in its additional part (3) is located a layer (5) of reagents. The layer (4) for placing the sample to be tested and the layer (5) of reagents are based on true gelatines.

Description

IDEΚΑΤ0ΚΑΤ0Ε ΤΕCΤ-USΤΡΟΤΡΟΪΒΟΪΒΟ FOR ΟΟΡΕΕΕΚΟΗΚΟΗΚΟΗΚΟΗΕΗΤΡΑΕΗΤΡΑΚΟΗ SUBSΤΡΑΤΟΒ ΕΡΜΕΗΤΑΤΕΡΜΕΗΤΑΤΕΡΜΕΗΤΑΤΒΗΒΗΧΧΡΕΑΚЙЙ

Glitter Technology

The present invention is not subject to 5 areas of biochemistry, or, more precisely, to indifferent gestational disorders.

PREVIOUS LEVEL OF TECHNOLOGY

Β the current time in connection with the use of

10 sοκοeφφeκτivnyχ leκeρsτvenny χ sρedsτv nοvyχ meτοdοv and treatment, intensification neοbχοdimοsτi κοnτροlya κeches τve πi- schevyχ προduκτοv, inτensiφiκetsiey ρe znοοbρzznyχ τeχnοlο- gichesκiχ προtsessοv in miκροbiοlοgichesκοy and πischevοy προ- myshlennοsτi shiρiτsya ποτρebnοsτ in seleκτivnyχ eκsπρess-

15 Enelysis. Β ρyade slucheev, neπρimeρ, πρi seχeρnοm diebe- Te, τaκοy eneliz (eκsπρess-anzliz glyuκοzy) yavlyaeτsya πρeκτiche sκi edinsτvennym and zhiznennοvezhnym κρiτeρiem οtsen- κi sοsτοyaniya bοlnοgο and eφφeκτivnοsτi προvοdimοgο leche- Niya. Β connection with the above is not observed at all

There are 20 (especially in medicine) in cheap, unreliable and selective enzymes biological active compounds.

Ηzibοlshee ρzsπροs τρanenie ποluchili οdnο- and mnοgο- κenelnye sπeκτροφοτοmeτρichesκie enelizeτορy, ρeelizuyu- Suitable izvesshnye meτοdy enelize, sρedi κοτορy ^χ φeρmenτnye

25 methods are in general more impaired and impaired, ensuring an inactive and high speed of reactivity under mild conditions. Οdneκο, iχ πρi- Menen τρebueτ isποlzοvznie, κeκ πρevilο, nesκοlniχ ρzsgvοροv ρeegenτοv, svyazenny ^χ with eτim προtseduρ iχ πρigο-

30 τοvleniya, dοaiροveniya vseχ ρesτvο ^'ροv, vκlyucheya eneliziρue- my οbρezets. UZBE UZSTEVYAHELGSTVE and, first of all, the use of complex, as it is called, the stereznenzny prypibaev, in this case it consumes energy. - 2 -

Bοlee πeρsπeκτivnymi yavlyayuτsya indiκeτορnye τesτ- usτροysτvz, οsnοvennye ne meτοdeχ suχοy χimii, chτο iο- κlyucheeτ τzκie οπeρztsii κzκ πρigοτοvlenie ρesshvοροv, iχ dοziροvenie, dοziροvznie eneliziρuemοgο οbρeztse and chτο 5 οsοbennο vezhnο not gρebuyuschie isποlzοveniya izmeρiτel- nyχ πρibοροv for ποluκοlichesgvennοgο znzlize. The principle of the action of geeky indiscriminate gesg-devices is based on a non-visual assessment of the intensity of the process and the absence of time.

10 Izvesτnο indiκaτορnοe τesτ-usτροysgvο for οπρedele- Nia κοntsenτρetsy subsτρegοv φeρmenτeτivnyχ ρeeκtsy (communication, Α, 2052057) between sοdeρzhzschee sοedinennye sοbοy ποdlοzhκu and πο at meρe οdin slοy ρeegenshοv and slοy for neneseniya eneliziρuemοgο οbρeztse, vzeimοdeysτvuyuschy

15 with the most closely related reagent layer. Β dan- nοm indiκaτορnοm gesτ-usτροysτve ποdlοzhκe vyποlnene as sveτοπροnitseemοy, gidροφοbnοy πlοsκοm lenshy, slοy ρeegenshοv vyποlnen nz οsnοve svyazuyuschegο veschesshve with ρes- πρedelennymi therein ρeegenshemi and πρiκρeπlen κ οdnοy πο-

20 versions of a hydroplanar ribbon, in particular a layer for carrying out an energizable hydroelectric cartridge is used,

On the one hand, in this test-ussvoe volume, in the country

25 προgeκeΕτ ρeeκtsiya, οgρenichen bottom πlοsκοsτyu gidροφοb- nοy πlοsκοy Leung sveρχu - gidροφilnοy τκznyu and πο lpg οdρedelyaeτsya gοlκο slοem ρezgenτοv, πlοsched κοτοροgο οgρenichene τem minimzlnym οbemοm znzliziρuemigο οbρez- tse (nesκοlκο miκροliτροv) κοτορy dοlzhen ποκρyvesh

30 the entire area of the layer of degenerations, which is generally necessary for the loss of layers of non-existent, non-existent and non-existent. By and large, especially, it complicates the process of making an indicator of the sixth-best condition.

35 On the other hand, in this indigenous shesh-ussrushshve you will need to protect the layer of the reagents κ - 3 - ποveρχnοsshi gidροφοbnοy πlοsκοy lenshy ποsρedsshvοm κleya, κοshορy in zzvisimοσshi οsh svοiχ svοysshv and sheχnοlοgii ne- incurring no influence mοzhvsh οκezyveτ τοlκο szmu ρeen- tion ne, ne and nο οbem, b κοshοροm οna προgeκeeτ, chshο decrease sheesh shοchnοssh izmeρeniya and sheκzhe you complicate the process of the indices of the sessh-ussrssve.

Εsche in dennοm indiκeshορnοm shessh-usτροysshve schishyve- of ρezulshaga enalize προvοdishsya only mοmenτ ρezvishi οκρzsni cheρez οπρed-ELENITE insheρvzl vρemeni, το esτ of meρenie προvοdigsya in κineτichesκοm ρezhime, chgο for ποlu- cheniya πρzvilnyχ and vοsπροizvοdimyχ ρezulτzτοv gρebueg ποsgοyansτve eκτivnοsgi φeρmenτοv in slοe ρeegengοv , which is subject to the C-Laws Dispatch indicator. Α still dennοm indiκeshορnοm shesτ-usshροysshve προve- denie enelize in κineτichesκοm ρezhime οbuslevlivzeτ neοb- χοdimοsg πρedvzρishelnοy sshendeρshizztsii φeρmenshοv πο iχ zκgivnοs τi, chτο πρivοdiτ κ udοροzheniyu geχnοlοgii due gοgοvleniya slοev ρeegengοv and chτο in svοyu οcheρed, ποvy sheeτ sebesτοimοsg yΗdiκeτορnοgο shessh -usr . Τeκzhe in dennοm indiκeshορnοm shessh-usshροysshve in The case of chee isποlzοveniya in κechesshve svyazuyuschegο veschesτve πορi- sτοy bumegi πρedyavlyayuτsya vysοκie τρebοvzniya κ her κechesτvu, το esτ nzbορu οπρedelennyχ φiziκο-χimi- chesniχ svοysτv, chgο _{οbyazyvaeτ.} . sτendeρτizοveτ eτi svοysτve (τοlschinu, diemeτρ πορ, tsveτnοsτ, nzbuχee- mοsτ and τοmu ποdοbnοe) πρi iοποlzοvznii bumzgi of ρez- nyχ πeρτy, chτο τaκzhe πρivοdiτ κ udοροzheniyu τeχnοlοgii izgοτοvleniya slοev ρeegenshοv and chgο in svοyu οcheρed, πρi vοdiτ κ ποvysheniyu sebesτοimοsτi indiκzτορnοgο τesτ -usst- ροysггз.

DISCLOSURE OF THE INVENTION

The main reason for the use of this product is the use of an electronic device for the connection of power components that have to be connected to other devices - -γ - veschesτvο slοya ρeegenshοv and slοy for nzneseniya enzliziρuemοgο οbρzztse would vyποlneny sheκim οbρzzοm, chshο ποzvο- would lilο uπροsshish sheχnοlοgiyu izgοshοvleniya slοya ρezgenshοv and slοya for neneseniya enzliziρuemοgο οbρe ztse and οsuschessh- 5 vig προvedenie enelize ποsle zeveρsheniya πρevρescheniya subsgρzτe in πρύdux.

It is possible to get a heme, which, in an indis- trial test system, is used to determine the concentra- tions of substrata of the fractions, which consist of interconnected interconnections.

10 and lοzhκu πο at meρe οdin slοy ρeegengοv, vyποlnen- ny ne οsnοve svyazuyuschegο veschesshvz with ρzsπρedelennymi therein ρeagenτemi and slοy for neneseniya znzliziρuemοg.ο οb- ρzztsz, vzeimοdeysτvuyuschy with nzibοlee blizκο ρesποlοzhennym slοem ρezgenτοv, sοglesnο izοbρeτeniyu ^{'ποdlοzhκe} izgοgοv-

15 lenz in the form of a separate extract, more. ^'Nnοy of τeρmοsτοy- κοgο προzρechnοgο meτeρizle and having οsnοvnuyu and πο lesser meρe οdnu dοποlnigelnuyu chzsτi, κοτορye προsτρensτ- vennο οτdeleny οdne οτ dρugοy, e in mοmenτ vzeimοdeysτviya οbρzzuyug zzmκnuguyu geρmeτichnuyu ποlοsg and from a κοτορyχ

20 οsnοvnοy chesτi ρzzmeschen slοy for neneseniya enzliziρuemο- gο οbρeztse, s dοποlniτelnοm in chzsτi - πο at meρe οdin slοy ρezgengοv, πρichem slοy for nzneseniya enelizi- ρuemοgο οbρeztse and binder veschesgvο πο at meρe οdnοgο slοya ρeegenτοv vyποlneny ne οsnοve isτinnyχ sgud-

25th.

Tselesοοbρeznο, chgοby in indiκeτορnοm τesτ-usτροysgve for οπρedeleniya κοntsenτρetsy subsτ ^'ρzτοv φeρmengzgivnyχ ρezκtsy in sluchee mnοzhesgve dοποlnigelnyχ chesτey ρzzem- nοy κzπsuly in κezhdοy of dοlοlniτelnyχ chesτey would

30 is allocated for a smaller layer of one layer of reagents.

Iron-ore, so that in an indi- vidual test device for the determination of the concentration of substances of the ingredients, there is no need to reduce the incidence of

35 The best solution is that you will improve the process of eliminating the layer of reagents and the layer for carrying - 5 - it is enumerable to simplify the process of producing indi- cating sessh-enhancing sys- 5 In addition, the inconvenient solution increases the amount of energy available when the subscription termination is completed, and the longer the service term is longer.

Г э κ κ ис ис ис

10 The following is a genuine 'excuse' which is explained by his understanding of the fact that ^: it is; performances and πρilehemes by my own, in the κο-ззρз::: _. Fig. Ι from ρ ρ ρ жж--в Д Д Д ин ин ди ди ин ин ин ин ин ин ин ин ин ин ин ин ин ин ин ин Д Д Д ин ин ин ин ин Д Д Д Д ин ин ин all

15 shivnyh reektsiy (in a separate rezerez), according to the invention; Fig. 2 - a general view of the indicator of a tesg-ussrusgvz for the determination of κκκΗΗшшш с с ^ ^ ^сссссс φ φ φ φ φ φ φ φ φ φ φ φ эээϊϊϊϊϊϊ с эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн эн энϊ энϊ эн эн эн- эн энϊϊ эн эн- эн эн- эн эн- эн эн-- эн leading for us to do.

20 ρzztsοm (in προdοlnοm ^'* ρezρeze) with οglasnο izοbρeτeniyu φig.Z - οbschy view iΗdiκeshορnοgο shessh-usτροysshve for οπρedeleniya κοntsenshρetsy subsshρz shοv φeρmensheshivnyχ ρeaκ tions πο φig.2 in mοmensh vzeimοdeysshviya slοya ρeegenshοv sο slοem for neneseniya eneliziρuemοgο οbρeztsz (in προdοlnο

25 ρezρeze) sοglesnο izοbρesheniyu φig.4 - οbschy view indiκzshορnοgο ^{'shessh-usτροysgve} for οπρedeleniya κοntsonτρetsy subsτρeτοv φeρmengegivnyχ ρeeκ πο φig tions. Ι with a separate encapsulation, which has two optional chesgi (in the local section), according to the invention

30 niu; φig.5 - οbschy view indyκeτορnοgο τesτ-usshροysshve for οπρedeleniya κοntsbnshρetsyy subsshρe shοv φeρmensheshivnyχ ρeen tions πο φig.4 sο snyaτymi κρyshnemi and enzliziρuemym οbρe tsοm (in προdοlnοm ρezρeze) with οglesnο izοbρesheniyu;

35 fig. B - general and indie gessh-ussstwe for - b - the division of the incentive of the substratum of the substances is ^χ in the process of fig.

5 Best Respect of the Invention

Indiκeshορnοe shessh-usshροysshvο for οπρedeleniya κοn- tsenshρetsy subsshρeshοv φeρmenshzshivnyχ ρeeκtsy sοdeρzhish ρezemnuyu κeπsulu I (φig.Ι) izgοshοvlennuyu of lyubοgο sheρmοsshοyκοgο προzρechnοgο mesheρizle, nzπρimeρ, πlesshmes-

10 sy. The infirm dieshesh napsules I am sharing the least possible subsection that has come down from the enlisted process. Sule Κeπ- I have οsnοvnuyu 2 and 3 dοποlnishelnuyu chesshi, προssh- ρznsgvennο οshdelennye οdne οsh dρugοy. Ч BASIC CHESHI 2 NZPSULA I placed in layer 4 to carry enaliziruemy

15 Obrazze, in its optional 3, is accommodated with a layer of 5 resignations. Cases 4 and 5 have been carried out for the sake of true, more reasonable, up to 35 ° C, which are not in good condition (for example, 10 solid gels). Fundamentally 2 and additionally - Chelny 3 Cheshpsy Kapsula I snebzheni sosvesvedenno svyshenno kryshke

20 mi 6.7.

There are two indicators that are more suitable for the division of secondary subsections of which are 2.3, which is 6, 7,

25 lysable obrazets 8.

Ηe φiguρe 3 πρedsgzvlenο indinegορnοe τesg-usτροy- sτvο for οπρedeleniya κοntsenτρztsy subsτρeτοv φeρmenτeτiv us ^χ ρezκtsy πο φig.2 with udzlennym enzdiziρuemym οbρeztsοm 8 mοmenτ vzzimοdeysτviya slοya (φig.Z) for applying

30 enalizable obrazets with a layer of 5 originals.

By the other hand, the performance of the index is more advanced for the purpose of sharing the benefits of the input signals. The key is

35 it is carried out in that way, that the separate voltages of I (fig. 4) contain more - 7 - One additional part 9, supported by two 10,11 upgrades. Β part 9 is divided into a layer of 12 ген reagents, which is in the pure form without the use of a binding material. Ηe φiguρe 5 πρedsshevl _(enο indiκashορnοe shesτ-5 usshροy- sshvο for οπρedeleniya κοntsenshρetsy .subsshρeshοv φeρmenshzshiv nyχ ρeeκtsy sο snyashymi κρyshκeSch "6, 7" sοοshveshsshvennο with chzsτey κρyshκemi 2,3 and 10, 11 with chesshi 9 ρzzemnοy κeπsuly I. Ηe slοe 4 ρreplaced by enalization уембρρецецецец 8.

This is a 6-year-old six-speed six-speed display.

10 for compilation of the benefits of a subsection of the changes in section 5 with a deleted ensemble for example 8 in the case of a loss of 4 (see section 5). 5D2 Results. Ches and 2, 9.3 Capsules I are consequently aligned and open closed

15 hermetic area of capsule I.

Inductive gestational devices for the determination of the center of the electronic components of the process in the form of 1, 2, 3 of the following method.

2 Main 2 and optional 3 parts

20 capsules I accommodate a corresponding layer 4 for carrying an enalizable obrazets and a layer of 5 reagents. The extinction of the unzenzhennyh layers 4 and 5 is due to the basic connections, because they are cut and the temperature is less than 35 ° C.

25 hemispheres. 2 and 3 Chzsτi κzπsuly I οτdeleny dρug οsh dρu- ge and κezhdaya of niχ zenρyshasοοτveτsτvennο κρyshκοy 6, 7. Κρyshκi 6, 7 πρedοχρznyayuτ sοοshveshsshvennο slοi 4.5 οsh ze- gρyazneniya, οsh meχznichesκiχ ποvρezhdeny and sluchee non πρedvidennοgο ρzsπlevleniya slοev 4 5 πρ and χρ

30 people I osh and ^χ above Kenya.

The division of profitable subsystems into indivisible second-step expansions by the following process.

From Cheshshi 2 capsules I I take off 6 and not 4

35 for carrying out the energy supply of the equipment - 8 - lennοe vρemya nesκοlκο κeπel ρesτvορz enzliziρuemοgο οbρeztse 8. Β τechenie eτοgο vρemeni in slοy 4 προisχοdiτ diφφuziya niznοmοlenulyaρnyχ sοedineny of eneliziρuemοgο οbρeztse 8. Κοlichesτvο προdiφφundiροvzvshegο subsτρzge ^'5 πρyamο προπορtsiοnelnο egο κοntsengρetsii in οbρeztse 8 (and diemeτρu chesτi κzπsuly I, sοdeρzheschey eτοτ slοy 4). After a certain period of time, the sample 8 being removed is removed with an increase in layer 4 by tearing or washing off with a mild water jet. Then, with part 3 of the capsule I remove

10 to cover 7 and connect it to chzgu 2, forming a closed hermetic space. Κeπsulu I ποmescheyuτ, nzπρimeρ in τeπluyu vοdu with τemπeρeτuροy not lower than 37 ° C and at ^'yshe 60 ° C, ρezulτzτe chegο sτudni slοev 4.5 ρesπlevlyayuτsya and sme- shiveyuτsya. Елен ρπΟедеделенеленеленκκκ эн эн эн эн эн эн эн эн эн энэ энэээ энэ энэээοοοοοοοοοοοοοο

15 tse 8, which was included in layer 4, as it was much better above, just in layer 5 of the regeneration and initiating a regular reaction. When this reaction is carried out, the process of recovery is processed. The intensity of the environment is directly increased by the concentration of substances in the environment.

20 Sample 8. After 5-15 minutes after the reaction, the mixture is intensified due to the mixture in capsule I with a standard glass. Thus, the disclosure of the invention 8 carries out, according to the invention, after the completion of the conversion of the substance to the manufacture,

25 wide modes of "end-to-end" mode.

The transmission of energy in the “end-to-end” mode, somewhat unknown, is more precise and does not end up with an incentive and active fragmentation. Consequently, even a significant reduction of the activity of enzymes (up to 80) in

30 that long-term breakdown of the capsules does not affect the results of the enzlize.

An indi cate Gesta for the separation of the subscriber subsystems in terms of performance in the case of 4,5,6 secured enzymes. The implications are

35 in general, another good part is 9 nzpsula I zepol- - 9 - I believe that there are 12 disagreements, with a fixed, indefinite, in its purest form, without the use of a binding substance. The lovers of the 9th Capsule I zenryvayush kryshkemi 10,11, the functions of the good are enzymatic above. 5 Separation of concentra- tions of subsystems of randomly generated indices with a tessergic drive is the same as the above.

According to the invention, the use of an optional part of 9 parts I allows you to create favorable conditions for 10 long-term adverse effects Consequently, for a greater number of re- generators, there are a few additional 9 parts that I would like to do to improve the results.

For the best understanding of the missing invention, we present the following concise examples of its existence. Ρime ρ I

The concentration of glucose was shared. 20 Weighed a wide mixture of the following sosgavz: zhelegin I g

ΓLΘΚΟZΟΟΚSIDZZE 20 mg phenol I 'mg

25 πеροκзидзе 20 mg

4-amino-entipyrine 2 mg

The resulting mixture was dissolved at 45 ° C in 10 ml of a phasophosphate buffered solution (0.05 Η) 7.1). For the production of layer 5 (fig.), The reagents' 2250 mark of 30 extended parses was extended for an additional period of 3, which is slightly longer.

For growth of layer 4, for carrying out a standardized 400-microlitel 10 of the yellow iron, 35 - 10 - and at a low temperature of 45 ° C, placed in the main part of 2 parts of I with an internal diameter of Ι2mm and closed by a 6.

The division of glucose concentrations was carried out by the following method. 5 Β the initialized sample 8 (Fig. 2) (for example I - a solution of 8 glucose) must be replaced after 5 minutes, 2 units I after 4, but after 5 minutes, 6 with the other part 3 of capsule I, containing a layer of 5 ree- 10 genes, it is also preliminarily crawled from it by a bullet 7.

According to I, the mixture was placed at a temperature of 50 ° C for 10 minutes, and, as a result, 4.5 (fig. 3) were mixed and mixed. After extracting Kapsula I from the thermosgage, the evolution of the eroded mixture in Exchule 15 I was improved with an extended, more common source of heat.

NOTE 2

Οπρedelyali κοntsenshρztsiyu eshilοvοgο sπiρshz. The following mixture was prepared: 20 gelshine I g elgogolsedise 20 mg perepoksidze 20 mg.

The resulting mixture was diluted at 45 ° C in 10 ml of an optional buffered solution (0, Η Η,8 7.8), containing I Μ 25 eshylenediaminesis. For layer 5 (fig. 3), a loss of 250 pounds for a larger upgrade is available for an optional part of 3 capsules I,. having an internal diamegr ,2 mm, and opened up at a height of 7, For the purpose of layer 4, to carry an enzyme-30, the mixture was prepared as follows: 10 mg phenol

4-amino-enantipyrine 20 mg seven-kerabaside salted 15 mg zhelegine I g

35 at a temperature of 45 ° C in 10 ml of a buffered buffer - II - ρ с ΙΜ ((0, ΙΜ ρΗ 7, 8). 700 μl of this hot product was placed in the main part of 2 capsules I with an external Ι2mm diester and closed in place 6.

Here, they shared the incentive of the eshilov.

5 as follows.

Ηe slοy 4 chesshi 2 neπsuly I, πρedveρishelnο ude- liv κρyshnu 6, ne 5 minush ποmescheli nesκοlnο κeπel zne- liziρuemοgο οbρeztse 8 (φig.2) sοdeρzheschy eτilοvy sπiρτ.

After an uzzenny intereval in the course of time

10 8 were drifted with energetic stimulation. Surprising the 7th part with part 3 of capsule I, part 2,3 connected and placed ne

10 minutes in a thermotherm ρ and 50 ° С. Уль Result layers 4 and 5

(игig.)) ρzsplavlyavlyali and mixed. After extracting the capsule from the temperature, the result was a response to the erupted

15 mixtures are available at the end of the season with a small, prefabricated, and used mixture.

NOTE 3

They shared the concentration of ethanol.

We made a mixture of the following systems:

20 eger-eger 0.3 g nikotinemidzdenindinule (ϋϋ. ⁺ ) 10 mg

The resulting mixture was dissolved at 50 ° C in 10 ml of glycine-CΗ buffer solution (0.0ΙΜ 3.5%). For φ layer 2, layer 12 (FIG. 4) of regenerators for an additional part

25 9 capsules I with an internal diameter of 7 mm, closed with one side of the bulkhead 10, placed 200 microns of the second and unbroken.

We made another mixture of the following systems: eger-egerz

30 dia

(ΚΑSh-dehydrogen-genase)

35 _^ Pοluchennuyu mixture enelοgichnο vysheοπisennοmu ρzsshvορya whether πρi 50 ° C in 10 ml φόsφeshnοgο buφeρnοgο ρzsshvορz (0,0ΙΜ - 12 - ρΗ 7.0). For φορmiροveniya slοya 5 (φig.4) ρeegenshοv 250 mκl πρigοshοvlennοgο ρesshvορe ποmescheli in dοποlnishel- hydrochloric chessh 3 κzπsuly I with vnushρennim dizmeshροm 7 mm and ze- κρyveli κρyshκοy 7. 5 For φορmiροveniya slοya 4 for neneseniya enzliziρuemο- gο οbρeztse in οsnοvnuyu chessh 2 Capsules I with an external diameter of 7 mm were placed at 300 μl hot (50 ° C) resh 0.3 г er-э ρ ρ н 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 (0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1 0,1. 6.

10 Zeshem οπρedelyali κοntsenshρztsiyu eshilοvοgο sπiρshe zne- lοgich ^'nο οπisennοmu in πρimeρe I.

This is one of the following examples, a methodology for the elimination of indigenous syshsshshyshys for the processing of concentrates of substances of substances

15 goals, on the one hand, and the essence of the invention, on the other hand, are used in the course of business. And one of the most unique experiences doesn’t require the use of any special option ^.

20 τeχnοlοgichesκοgο οbορudοveniya and sledοvzτelnο, mοzheτ byτ vyποlnenz with πρimeneniem sτendeρτnyχ neuchnyχ πρibο- ροv, geκiχ neκ scales, ρΗ-meτρ, ρe zlichnye dοzzτορy, τeρmο- sτzg and gοmu ποdøbnοe, sοsgevlyayuschiχ neοbχοdimy least τeχnichesκiχ sρedsτv sοvρemennοy biοχimichesκοy lebορeτο-

25 r. Znechiτelnοe uπροschenie τeχnοlοgii izgοτοvleniya invariant diκzτορnyχ gösgen district-usτροysτv for οπρedeleniya κοntsenτρztsy subsgρzτοv φeρmengeτivnyχ ρeeκtsy ποzvοlyaeτ legκο nele- diτ not τοlκο messοvοe iχ προizvοdsτvο, nο and gοτοviτ melye πeρgii for eκsπeditsiοnnyχ ρebοτ is lebορzτορy

30 with the aim of introducing an emergency knowledge of the methods of "dry chemistry" of practical any connection, with the aid of the separation of the object of a non-existent disturbance. Eτο ποdτveρzhdzeτsya πρivedenny- E πρimeρzmi κοτορy in ^χ for πρigοτοvleniya indiκzτορnyχ

35 gas-powered devices for the determination of glucose and synergistic concentrations were used widely known reagents and methods. - thirteen -

Bοlee gοgο for ποlucheniya κοlichesgvennyχ ρezulτeτοv with ποmοschyu dennyχ indiκeτορnyχ shessh-usshροysshv with οshibκοy not πρevysheyuschey οshibκu meshοde, mοguτ bysh isποlvzοveny sπeκsh- ροφοτοmeshρichesκie enelizeτορy shiροκοgο προφilya, ποsκοl- 5 κu nοnsshρuntsiya usshροysshv sheκοvz, chshο ποzvοlyaesh iχ φοgο- meshρiροvenie προsvesh ne.

Another important indifferent indicator is that it is more convenient for the purpose of sharing the benefits of the subsystems and the efficiency of the processes is possible.

10 lize with egο ποmοschyu with ποsshοyannοy minimzlnοy οshibnοy Teh same cheρez dlishelny sροn χρeneniya in Sechen κοshοροgο mοzhesh προizοyshi suschesshvenneya (dο 80) ineκshivetsiya used ποlzοvznnyχ φeρmenshοv, ποsκοlκu προvedenie enzlize οsuschesshvlyayush in ^ρezhime, κοnechnοy shοchκi. "Τeκim οbρezοm,

15 saving even 20% of the original active components you use by adding a complete range of sub-components to the backup.

The best solution is that you get the most comfortable and comfortable conditions for an extended stay.

20 changes of the reagents without changing their эн св эн эн эн,,,,,,,,,, зв зв зв, which means that you have the best experience in terms of high accuracy.

Indicated πρ

You can use this invention in a wedge of 25 ceremonial studies, food and microbiological research, which is beneficial.

Claims

- 1 - ΦΟΡΜУЛΑ ЙЗΟБΡΕΤΕΗИЯ

1. An indicative test device for the determination of concentra- tions of substrata of functional sections, which is connected less to the extent necessary

5 οdin slοy (5) ρeegenτοv, vyποlnenny ne οsnοve binders schegο veschesτve with ρesπρedelennymi therein ρeegengzmi and slοy (4) for neneseniya enzliziρuemοgο οbρz ztse, vzeimοdeysg- vuyuschy with NZ ibοlee blizκο ρesποlοzhe nnym slοem (5) ρeegen- ΤΟB, οτlichzyuscheesya gem, which is available in the form of

10 ρezemnοy κeπsuly (I), from vyποlnennοy τeρmοsτοyκοgο προz- ρechnοgο meτeρielz and having οsnοvnuyu (2) and at πο meρe οdnu dοποlniτelnuyu (3) chzsτi, κοτορye προsgρenssh- vennο οshdeleny οdne οsh dρugοy, s mοmensh in vzeimοdeysshviya οbρz zuyush zemnnushuyu geρmeshichnuyu ποlοssh, and from в

15 basic cheshes (2) is replaced by a layer (4) to carry an energy supply, but in addition an additional cash is (3) - there is a lower connection - in the lower case of the single layer (5) the results were fulfilled

20 ns the basis of the most recent.

2. INDICATOR TEST-INSTRUMENTS πο π. Ι, an expandable gem, which in the case of a large number of additional cassules (I) is available in each of the optional caches (3.9) is replaced by an additional 12 (5)

25 sev. .

3. An indispensable best-selling one from ππ. 1.2, Chez οshlicheyuscheesya ^'m chshο οsnοvneya (2) and dοποlnishel- nye (3,9) chzsshi ρezemnοy κeπsuly (I) snzbzheny κρyshnemi (6, 7,10,11).