WO1991010910A3 - Procede de traitement enzymatique de substrats - Google Patents

Procede de traitement enzymatique de substrats Download PDF

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Publication number
WO1991010910A3
WO1991010910A3 PCT/EP1991/000086 EP9100086W WO9110910A3 WO 1991010910 A3 WO1991010910 A3 WO 1991010910A3 EP 9100086 W EP9100086 W EP 9100086W WO 9110910 A3 WO9110910 A3 WO 9110910A3
Authority
WO
WIPO (PCT)
Prior art keywords
fusion protein
supporting base
substrate
codes
brought
Prior art date
Application number
PCT/EP1991/000086
Other languages
German (de)
English (en)
Other versions
WO1991010910A2 (fr
Inventor
Rainer Rudolph
Erhard Kopetzki
Stephan Fischer
Adelbert Grossmann
Baerbel Hoell-Neugebauer
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4001508A external-priority patent/DE4001508A1/de
Priority claimed from DE4002636A external-priority patent/DE4002636A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of WO1991010910A2 publication Critical patent/WO1991010910A2/fr
Priority to NO913673A priority Critical patent/NO913673D0/no
Priority to FI914389A priority patent/FI914389A0/fi
Priority to KR1019910701147A priority patent/KR920701822A/ko
Publication of WO1991010910A3 publication Critical patent/WO1991010910A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • AIDS & HIV (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Afin de soumettre des substrats à un traitement enzymatique, on met le substrat à traiter en contact avec un biocatalyseur obtenu en combinant un gène de codage pour une substance biologiquement active avec un fragment d'ADN de codage d'un peptide de liaison capable d'interaction avec un matériau de support, de manière à obtenir une protéine de fusion. On insère ensuite celle-ci dans un vecteur approprié, on la transforme dans un organisme approprié, on met en culture ledit organisme, on en dissout les cellules et on met le lysat contenant la protéine de fusion en contact avec un matériau de support capable de se lier au peptide de liaison. La protéine de fusion se lie au matériau de support par interaction intermoléculaire, ce qui permet d'obtenir le substrat enzymatiquement traité.
PCT/EP1991/000086 1990-01-19 1991-01-18 Procede de traitement enzymatique de substrats WO1991010910A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
NO913673A NO913673D0 (no) 1990-01-19 1991-09-18 Fremgangsmaate for enzymatisk behandling av substrater.
FI914389A FI914389A0 (fi) 1990-01-19 1991-09-18 Foerfarande foer enzymatisk behandling av substrat.
KR1019910701147A KR920701822A (ko) 1990-01-19 1991-09-18 기질의 효소적 처리 방법

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DEP4001508.4 1990-01-19
DE4001508A DE4001508A1 (de) 1990-01-19 1990-01-19 Verfahren zur enzymatischen behandlung von substraten
DE4002636A DE4002636A1 (de) 1990-01-30 1990-01-30 Expression von hiv1- und 2-polypeptiden und deren verwendung
DEP4002636.1 1990-01-30

Publications (2)

Publication Number Publication Date
WO1991010910A2 WO1991010910A2 (fr) 1991-07-25
WO1991010910A3 true WO1991010910A3 (fr) 1991-10-03

Family

ID=25889228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/000086 WO1991010910A2 (fr) 1990-01-19 1991-01-18 Procede de traitement enzymatique de substrats

Country Status (6)

Country Link
EP (1) EP0464184A1 (fr)
JP (1) JPH04503610A (fr)
KR (1) KR920701822A (fr)
AU (1) AU633686B2 (fr)
CA (1) CA2047235A1 (fr)
WO (1) WO1991010910A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0089626A2 (fr) * 1982-03-19 1983-09-28 G.D. Searle & Co. Procédé pour la préparation de polypeptides à l'aide d'un polymère d'acides aminés chargés et une exopeptidase
EP0282042A2 (fr) * 1987-03-10 1988-09-14 F. Hoffmann-La Roche Ag Protéines fusionnées et leur purification
EP0286239A1 (fr) * 1987-03-10 1988-10-12 New England Biolabs, Inc. Production et purification d'une protéine fusionnée d'une protéine de liage
EP0289339A1 (fr) * 1987-05-01 1988-11-02 Cambridge Bioscience Corporation Détection d'anticorps de virus immuno-déficiant humains
EP0326100A2 (fr) * 1988-01-29 1989-08-02 Abbott Laboratories Essais et dispositifs à capture d'ions
EP0339389A1 (fr) * 1988-04-25 1989-11-02 F. Hoffmann-La Roche Ag Moyen auxiliaire de test
EP0348780A2 (fr) * 1988-06-30 1990-01-03 Hoechst Aktiengesellschaft Procédé pour l'obtention de protéines

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0089626A2 (fr) * 1982-03-19 1983-09-28 G.D. Searle & Co. Procédé pour la préparation de polypeptides à l'aide d'un polymère d'acides aminés chargés et une exopeptidase
EP0282042A2 (fr) * 1987-03-10 1988-09-14 F. Hoffmann-La Roche Ag Protéines fusionnées et leur purification
EP0286239A1 (fr) * 1987-03-10 1988-10-12 New England Biolabs, Inc. Production et purification d'une protéine fusionnée d'une protéine de liage
EP0289339A1 (fr) * 1987-05-01 1988-11-02 Cambridge Bioscience Corporation Détection d'anticorps de virus immuno-déficiant humains
EP0326100A2 (fr) * 1988-01-29 1989-08-02 Abbott Laboratories Essais et dispositifs à capture d'ions
EP0339389A1 (fr) * 1988-04-25 1989-11-02 F. Hoffmann-La Roche Ag Moyen auxiliaire de test
EP0348780A2 (fr) * 1988-06-30 1990-01-03 Hoechst Aktiengesellschaft Procédé pour l'obtention de protéines

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Biotechnology, Band 7, Nr. 6, Juni 1989, (New York, US), E. Ong et al.: "Enzyme immobilization using the cellulose-binding domain of a cellulomonas fimi exoglucanase", Seiten 604-607 *

Also Published As

Publication number Publication date
AU7072491A (en) 1991-08-05
JPH04503610A (ja) 1992-07-02
EP0464184A1 (fr) 1992-01-08
KR920701822A (ko) 1992-08-12
WO1991010910A2 (fr) 1991-07-25
CA2047235A1 (fr) 1991-07-20
AU633686B2 (en) 1993-02-04

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