WO1990013311A1 - Vaccine for prophylaxis and treatment of microsporia caused by the pathogen microsporum canis - Google Patents

Abstract

A vaccine for prophylaxis and treatment of microsporia caused by the pathogen Microsporum canis represents a suspension of microsporia of immunogenic strain Microsporum canis in a 0.9 % solution of sodium chloride in a quantity of 25-50 m [million] of microconidia per 1 ml of said solution, including no less than 15 m/ml of colony-forming microconidia.

Description

 ΒΑΚЦΗΑ FOR ПΡΟФИЛΑΚΤЙΚИ AND ЛУЧΕΗИЯ ΜИΚΡΟСПΟΡИИ, ЫЗΒЖЮ ЭДЗБУДΤΕΜ Μ-ΪСΚΟЗΡΟISH СΑΝΙ5

Area of technology

Ηasτοyaschee izοbρeτenie οτnοsiτsya κ οblaeτi veτeρi- naρii and τοchnee κ nοvοy vaκtsine for προφilaκτiκi and leche- Nia miκροsπορii, vyzvannοy vοzbudiτelem Μϊsgοzροgit sa ^η ιz.

PREVIOUS LEVEL OF TECHNOLOGY

Dermatoses of animals and their environment, in particular, the distribution of fur animals, animals, domestic animals, are spread throughout the world.

Removing the furry animals from the meat reduces the quality of the products, disrupts the meat sales in the industrial environment. Sick living animals are a constant threat and source of disease spread among live and contacting people.

Β nasτοyaschee vρemya izvesτna vaκtsina for προφilaκ- τiκi and treatment τρiχοφiτii zhivοτnyχ, πρedsτavlyayuschaya sο- bοy slurry miκροκοnidy immunοgennοgο shτamma form τηsϊιο- ρu-οη teη ± ορu g ^ es Β 0 9 yes ρaSΤΒθρe ΗaτρiYa ΧLθρI- with ρΗ 6,2-7 , 0 in the amount of 15-25 million micronidia per I ml of solution (υз, Α, 4368Ι9Ι).

Οdnaκο uκazannaya vaκtsina naχοdiτ πρimenenie τοl- κο to treat τρiχοφiτii, vyzvannοy vοzbudiτelem Τgϊsϊιο- ρu ± οη teη ορϊιu ^ ag ^ es and mοzheτ byτ isποlzοvana to treat miκροsπορii.

DISCLOSURE OF INVENTION

Β οsnοvu izοbρeτeniya ποlοzhena task gguτem investigated dοvaniya κulτuρy mϊsgοzροgit sοzdaτ nοvuyu vaκtsinu for προφilaκτiκi and treatment miκροsπορii, vyzvannοy vοzbudiτe- Lemma Μϊsgοaροd _'sa ^η ϊz um.

Task ρeshena τem, chτο vaκtsina for προφilaκτiκi and treatment miκροsπορii, vyzvannοy vοzbudiτelem ΜΪSΓΟ- zροgit saηχs, sοglasnο izοbρeτeniyu, πρedsτavlyaeτ sο- όοy slurry miκροκοnidy immunοgennοgο shτamma form mϊs- gοzροgit saπ of ± 0.9 # -nοm ρasτvορe naτρiya χlορida with ρy - 2 -

6.2-7.0 in the amount of 25-50 million microconidia per I ml of solution, including a large amount of at least 15 million / ml of mycobacteria. 5 The inventive vaccine is predominantly suspended in the form of a suspension of micronidiums and contains a suspension of the immunogenic immunostimulant strain. sa ^η де from the party

06/28/188 in the All-Union collection of industrial microorganisms of the All-Union scientific and research institute 10 of the genetics and breeding of the world ’

To save a large amount of product, the vaccine also contains a protective medium of the following composition in mass. ^ G of waste or sugar 10.0-40.0. 15 gelatin 2.0- 3.0 water remaining in the amount of I ml of protective medium for 300-1500 million microcranidiums.

A dry vaccine in terms of appearance 20 provides a very clean, dry mass of green color.

It is available in glass flasks with a content of 1-200 doses. Each quantity is indicated by a quantity of doses.

Included with the vaccine is a flask with a distributor.

The vaccine is suitable for use during the first year. 25 The temperature is 2 ° C and 2 ° C.

Before you use it, you get it in the consumer. They use the vaccine for the treatment and treatment of mycosis in fur animals, animals, domestic animals.

The vaccine is harmless, active, does not cause 30 diseases in healthy animals, is safe for veterinary specialists and has been serviced by the medical staff.

BEST MODE FOR CARRYING OUT THE INVENTION

Declare a vaccine to immunize foxes, having been tested since 30 days of age, people from 4-5 days old, 35 days old, because of dogs and dogs from 2 - 3 months. I have completely entered the muscle area in the thigh twice with an interval of 7-10 days in the following doses: - 3 -

PREPARATORY TREATMENT DOSE DOSE (ml) (ml)

Young foxes

5 months 1-4 months 1.0 2.0 older than 4 months 2.0 3.0

Sunday from 45 days of age 1.0 1.0

Dogs 2-6 months old. 0-2.0 2.0-3.0

10 older than 6 months 1.0-3.0 2.0-4.0

Since 3 months. 1.0 2.0

After 20-30 days, the livestock receives intense immune activity for at least a year. In general, it has a curative effect, which is

15 1-3 weeks after immunization, and are expressed at the same time as the patient has been removed and full of human hair loss. With this, the use of medicaments is not necessary. immunization of the claimed vaccine

20 lively unfavorable formations last for one to two months, completely treat the disease, cure the sickly livelihood. The destruction of the young-wounded young man makes it possible in the following years to maintain the lasting well-being of the community of the empire.

25 The use of the claimed vaccine allows to exclude: reduction of the quantity of cheap raw materials; the meat product industry of the population; Scanning Ferm; restraint of tribal livestock sales; costs of medication; infection of people; external pollution

30 wednesdays.

The method of manufacturing the claimed vaccine is carried out as follows.

On the quality side of the immunogenic state, the program uses the strain of the benefit of £ £ -400. Indicated

The 35th strain was obtained as a result of the multi-penalized method of the most rapid fastening of a small bend with an active accumulation of micronidium.

The Storm of the Security Council of the United States of America 400-400 is characterized by the following properties:

- 4 - Medical Signs. A mature 20–25-day-old culture is provided by certified direct or curved spinning dykes with a width of 2–6 μm, numerous short-shaped, pear-shaped, 2–5 mm. In the 30-35 day old culture, there are intercalary chlamydrates. This is one of the most rare and old schools in the world. Cultural Signs. The strain is cultivated on must-agar and agnar of Genneberg.

When sowing in small cups, the indicated varieties and incubations are at 26-28 ° C; The dashboard at the front of the premises is not damaged, but in the center is yellow. The diameter of the bed for 18 days reaches 80-90 mm.

Pρi ποseve sποροvοy susπenzii on suslο-agaρ in chashκaχ Peτρi.κ 15-th day inκubiροvaniya πρi 26-28 ° C ρaz- vivaeτsya οdnοροdnaya πο κulτuρalnym πρiznaκam ποπulya- tion, πρedsτavlennaya κοlοniyami οdnοgο τiπa: baρχaτisτο- ποροshisτye vyπuκlye white κοlοnii, nesκladchaτye, ρasτu - The surrounding area is obsolete, the reverse side of which is generally not affected by the condition of the heart, but in the center is light-colored. The diameter on the 15th day is 35-50 mm.

Proprietary properties. When applied to a skyscraper, a skillet or a pig of a homogenized live agaric for 10-12 days, separate flakes are detected. Self-release occurs on day 15–25. immunogenic properties. Pρi dvuκρaτnοm vnuτρimy- shechnοm administered κροliκam vaκtsiny, yazgοτοvlennοy of πο- luchennοgο shτamma in dοze, sοdeρzhaschey 20-50 million zhivyχ miκροκοnidy, y zhivοτnοgο φορmiρueτsya nalρyazhenny immunoreactive niτeτ κ ποsleduyuschemu zaρazheniyu miκροsπορiey προdοlzhi- τelnοsτyu least 1.5 leτ (observation sροκ )

The indicated strain is distinguished by the absence of physiological weakness and the absence of infection. - 5 - between animals, high activity of accumulation of microcranidia and immunogenicity with intramuscular administration of animals. With the proposed strain, it is possible to manipulate the device in labs, in the same way as in the case of free conditions, without a risk for the health of the service provider.

Β The poor condition and the free access to the device are free of charge.

The preparation of a vaccine includes the following operations: the preparation of food medium; protection environment; preparation of a medium for the control of a vaccine; sowing and cultivation of vaccine culture; I’ll remove and homogenize the gates, connecting the homogenate with a protective environment; the finished product in flasks; materialization of material; purchase and etiquette of flasks; The end of the vaccine.

Κaaduyu seρiyu vaκtsiny ποdveρgayuτ κοnτροlnοy προ- veρκe on ρasτvορimοsτ, οsτaτοchnuyu vlazhnοsτ on chisτο- τu in baκτeρialnοm οτnοshenii and πlesnevye gρiby, sοdeρ- Contents and κοlοnieοbρazuyuschuyu sποsοbnοsτ miκροκοnidy, bezvρednοsτ and immunοgennοsτ. Those who have come to the end of the series are used for immunization. For a better understanding of the present invention, the following methods of obtaining a vaccine and its testing are given. Ρime ρ I

Production of a vaccine series in the amount of 300-350 thousand. dose For this 200–250 flat flasks (1.5 liter flask volume) with mustard agar (large quantities of 300-350 ml in a single flask), a crop of 6.2–6.8 is used for cultivation. Bulbs survive in the terrestrial area at a temperature of 26-28 ° C for 12-25 days. At the end of the cultivation, the gibber is removed, transferred to the chamber of the homogenizer and added to the sterile distilled water from the calculation of 200-300 ml of water for removal from 5-7 gaps. With this, a homogenate with a content of 300-1500 million / ml of micronidium is produced. - 6 -

The homogenate connects with protective food (containing 2-3 wt.% Of gelatin and 10-20 wt. Of sugar) at a ratio of 1: 1 (+ 25), divorces the bottle and divorces it. There are two types of medicine in the amount of 200 to 200 doses (one dose of the vaccine contains 25-50 mln / ml of micronidium, from which at least 15 mln ml)

The process of freezing takes place immediately after pouring the material into a low temperature refrigerating unit minus 40-50 ° С and freezing it for 8. Then the cassettes with flasks are placed in a sublimation chamber. Dry it and heat it up to plus 30-40 ° С and a stable vacuum. After reaching a temperature of 8 ° C to + 0 ° C, heating by heating reduces it to 25 ° C and maintains drying.

Mostly, it is a matter of personalized material, which is only a little after a dilution of $ 0.9 - that is a slight increase in average consumption of 6.2 mln. at least 15 million / ml.

Βaκtsinu ποdveρgayuτ κοnτροlnοy προveρκe on ρasτ- vορimοsτ, οsτaτοchnuyu vlazhnοsτ, sοdeρzhanie and κοlοnie- οbρazuyuschuyu sποsοbnοsτ miκροκοnidy on chisτοτu in baκτe- ρialnοm οτnοshenii and πlesnevye gρiby, bezvρednοsτ and immunοgennuyu aκτivnοsτ.

NOTE 2

A test was conducted on the immunization of the population. Experience has been used 116 users. Immunization of the claimed vaccine has shown harmlessness and activity. Two-way immunized suppressants survived an experimental infection with virulent strains of negative illness after 1.3 and 6 months after immunization.

The next step was the test of the claimed vaccine on cell foxes. 83 (1.5-2 months) puppies of the foxes were opposed to the claimed vaccine. Ующие The next few months of observation, none of the lively animals didn’t get sick, all the while they were in contact with the sick or were in an infected state - 7 - κаχ. With the curative goal of immunization, 4 female foxes and 46 puppies of foxes were sick, sick with mycosis. All the animals were brought in between 21-40 days. The tests were carried out at a fox farm, where a hearing was recorded for the microsperium (the exciter was excreted). For therapeutic purposes, the vaccine was tested on 182 puppies of silver-black foxes Ι, 5-3 months old. The vaccine was administered twice with an interval of 7-12 days in the rear end at a dose of 1-2 ml. Chakzin was named for animals with a different degree of defeat. Separate animals had up to 2-35 disposed parties, were located on the head, legs, open, and the body. After 10-15 days at the animals ’majority, the inflammatory events on the affected areas fell, became darker, got out of focus and lost, and were excreted. All the puppies recovered κ 30-45 days.

54 dogs were also treated, and 67 different diarrheas, more painful mixtures were treated. All of them survived after the introduction of the vaccine after 22-45 days. At the same time, vaccines have not been used with other medicinal products.

Preventive immunization 6074 foxes were bent. For the entire period of incidents within seven months (before the slaughter of animals), not one of the vaccines of the living did not become ill with mikrospresriyu. Every year, young people in the next few years were supported by good health benefits at this site. Prophylactic doses were made for puppies of foxes, prescriptions and foxed hybrids 1-4 months. Age - I ml, older than 4 months. and adult animals - 2 ml. Therapeutic doses are 2–3 ml. A second vaccine was administered after 7–10 days, at the same dose in the same rear end, with an internal side. The conditions of the animals, which are not favorable for the environment, had a total of 88,369 live animals (10 functions), including a score of 501. All animals were returned for 18-26 days without additional medical knowledge. - 8 - After the treatment. The effective efficiency was $ 93.74 up to $ 100. The test results are presented in the table.

5 Intended use

The inventive vaccine finds an application for προ-fila τ cility and treatment of the microsystem caused by the causative agent Μ ^{χ, which} saves the disease in live animals, in the home.

- 9 -

Table

ΤΑΤ ZULI TEST SECOND BACTION

Homemade lively disease (~ ο φ ω ~ ω (quantifiable physical effects)

LIVES) are ^χ )

Βsegs> in the Β С С С С Β Β -this day the month of the month: in% of the good month

I fox ϊ 7220 - - - - 100 calendar \ 8014 - - - - 100

2 months 6388 - - - - 100

3 fox 3283 395 96 - 2.92 97.08

4 fox 13857 65 43 - 0.31 99.69

5 Fox] 8879 - - - - 100 woods> 2314 - - - - 100 hybrids] 954 - - - - 100

6 fox 8700 - 3 - 0.03 99.97

7 fox 7670 - 6 - 0.08 99.92

8 fox 9426 - - 6 0.06 99.94

9 fox 7670 16 8 - 0.01 99.99

10 fox 3994 25: 250 - 6.26 93.74

Егegοο 88369 501406 6 0.74 99.26

Claims

- 10 -ΦΟΡΜULΑ IZBΟIA

1. A vaccine for the treatment and treatment of microbial disease caused by the causative agent of the disease,

5 χaρaκτeρizuyuschayasya τem, chτο οna πρedsτavlyaeτ sοbοy slurry miκροκοnidy immunοgennοgο shτamma vdda Μχsgοzροgshπ saηχz 0.9 ρasτvορe naτρiya χlορida with ρΗ 6,2-7,0 in κοliche- sτve 25-50 million miκροκοnidy on I mL uκazannοgο ρasτvορa in τοm including κοlοnieοbρazuyuschiχ mycροκοnidium not less than 10 15 million / ml.

2. The vaccine πο π. I, which is characterized by the fact that, in suspension of the suspension of micronides, it contains a suspension of micronidia of an immunogenic strain that is in danger of being discharged.

15 muscular microorganisms of the Soviet Union research and development institute of genetics and breeding of industrial microorganisms and foreign countries for me? -400. .

3. The vaccine πο π. I, which is subject to storage, that is, 20 for storage of a large amount of equipment, it also contains a protective medium of the following composition in mass. : sorbitol or sugar 10.0-40.0 gelatin 2.0-3.0 other water,

25 in quantity of I ml of protective medium for 300-1500 million micro-

ΚΟΗIDIYO.