WO1990005906A1 - Detection mixte d'analyte a l'aide d'un absorbant - Google Patents

Detection mixte d'analyte a l'aide d'un absorbant Download PDF

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Publication number
WO1990005906A1
WO1990005906A1 PCT/US1988/004059 US8804059W WO9005906A1 WO 1990005906 A1 WO1990005906 A1 WO 1990005906A1 US 8804059 W US8804059 W US 8804059W WO 9005906 A1 WO9005906 A1 WO 9005906A1
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WO
WIPO (PCT)
Prior art keywords
reaction zone
absorbent
liquid
means defining
control
Prior art date
Application number
PCT/US1988/004059
Other languages
English (en)
Inventor
Paul Christopher Brooks
Erwin Workman
Quentin J. Tonelli
Original Assignee
Idexx Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idexx Corporation filed Critical Idexx Corporation
Priority to PCT/US1988/004059 priority Critical patent/WO1990005906A1/fr
Priority to EP19890902484 priority patent/EP0442872A4/en
Priority to JP50236589A priority patent/JPH04502961A/ja
Publication of WO1990005906A1 publication Critical patent/WO1990005906A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/065—Valves, specific forms thereof with moving parts sliding valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se

Definitions

  • This invention relates to devices and methods for detecting an analyte, for example, using an immunoassay.
  • An analyte in a sample may be detected by treating the sample with various reagents, such as labeled immunological binding partners to the analyte and reagents to enable detection of the label. Often, the sample must be washed between administration of various reagents.
  • An accurate assay often depends on controlling, the amount of reactants exposed to the sample and the duration of the reactions talcing place. It is desirable to control these variables in a way that enables use of the assay kit by individuals of widely varying skill and experience, so that variations in individual technique do not materially alter the result. Consistency and accuracy can be difficult when a color-generating indicator must be evaluated, particularly if any background color is generated.
  • Test reagents should be tested to verify their activity, which may erode over time. Also, it is desirable to reduce the time necessary to perform the assay. It is desirable to have the ability to assay extremely small sample volumes with relative low concentrations of analyte, and/or to detect relatively small differentials in analyte concentrations. Finally, it is desirable to assay whole blood samples without complex centrifuging equipment.
  • One method for adding and washing reagents in an immunoassay uses an absorbent material to move liquid washes and reagents through a solid substrate (such as a membrane) to which other reactants are immobilized.
  • Cole et al. U.S. 4,246,339 discloses an immunoassay test device including sorbent material 18 for drawing liquid through a micropor ⁇ us membrane 22 at the bottom of a test well 20.
  • the sorbent material is resiliently biased away from the membrane, and it draws liquid through the membrane only when the two are forced together to overcome the bias.
  • Sorbent material 18 comprises a surface layer 36 which is hydrophobic and a bulk portion 38 which is wettable. Reagents are added serially to the test well and, after each reagent has been in the well for a prescribed time, the membrane and sorbent material are forced together to draw off liquid before the next reagent is added.
  • Tom U.S. 4,366,241 discloses an immunoassay device having two bibulous zones, an analyte binding partner being non-diffusively fixed in the first zone (the "immunoabsorbing zone"), and the second layer being a reservior zone, either directly or indirectly in liquid-receiving relationship with the first zone to pull liquid through and out of the first zone.
  • the immuno bsorbing zone is a portion of a flat test strip which is exposed by a hole in a protective coating.
  • Hossom U.S. 4,623,461 discloses a test device having a filter which feeds a specimen to a flat absorbent material having a reaction zone surrounded by peripheral zone.
  • An annular ring 40 of absorbent material is positioned around the peripheral zone, so that fluid is drawn radially outward from the reaction zone, through the peripheral zone, and into the absorbent material.
  • Bagshaine U.S. 3,888,629 discloses an assay device having a matrix pad 17 which may be pre-treated with one binding partner. Absorbent material 23 is forced into intimate contact with matrix pad 17 to increase the speed of filtration through the matrix pad.
  • Kondo U.S. Pat. 4,270,920 discloses multiple reagent layers arranged on a single horizontal support. A porous spreading layer (40 in Fig. l or 41 and 44 in Fig. 3) spreads the sample as it moves into the reagent layers, so that a relatively small sample volume will be spread evenly over the reagent layers.
  • test devices comprising positive and negative control spots on a test pad or slide.
  • an immunoassay device comprising positive and negative control spots. Separation of the spots is maintained by spotting the reagents a sufficient distance from each other.
  • One aspect of the invention generally features a test device having a control absorbent in liquid-transferring contact with an aqueous permeable, aqueous insoluble reaction zone, adapted to retain the detectable reaction product formed when analyte in the sample is treated with at least one liquid reagent.
  • the control absorbent has a predetermined, limited, standard, liquid-absorbing capacity, and it is adapted to absorb a predetermined volume of liquid from the - it - sample after the sample passes through the reaction zone. That predetermined, limited, standard volume is selected to enable reliable detection of the analyte, taking into account the reagent volumes and concentrations present, the mode of detecting the reaction product, and the analyte concentration level at which discrimination is desired.
  • control absorbent and the means defining a reaction zone are positioned i the device in the manner that, by filling the control absorbent to capacity, it effectively meters a predetermined limited flow between a first region of the reaction zone, which is exposed to receive liquid sample, and a second region thereof, spaced from the first region, which is in liquid transferring relation with the control absorbent.
  • a second aspect of the invention generally features a device that includes: an absorbent reservoir; means retaining the absorbent reservoir in a first position spaced apart from liquid-transferring contact with the control absorbent or the reaction zone; and means moving the absorbent reservoir from the first position to a second position, in which the reservoir is in liquid-transferring contact with the control absorbent and/or the reaction zone.
  • the two aspects can be combined in a single device so that the control absorbent draws the- predetermined sample volume into the reaction zone while the absorbent reservoir is in the first position; then the absorbent reservoir is moved into the second position, and the means defining a reaction zone is contacted with at least one of the liquid reagents which is drawn through the reaction zone into the absorbent reservoir.
  • the absorbent reservoir In the first position the absorbent reservoir is retained above the reaction zone, and it is moved from the first position to the second position, where it rests and is retained in liquid transferring contact above the reaction zone.
  • a plurality of liquid reagents can be drawn upwardly through the reaction zone into the absorbent reservoir without additional reservoir movement.
  • a latch means can be used to restrain movement of the absorbent reservoir from the second position back to the first position, so a plurality of liquid reagents can be introduced to the reaction zone while the absorbent reservoir is latched in the second position.
  • the device also can include a means to release the latch after the detectable product is formed, so th-e absorbent reservoir is returned to the first position while the reaction product is being detected.
  • At least one reactant participating in a reaction to form the detectable product is included in the reaction zone; for example, a specific binding partner for the analyte can be included in the reaction zone to trap the analyte there.
  • the reaction zone is impermeable to the analyte.
  • the reaction zone is defined on a flat member, and the two regions of the zone are opposite faces of the member
  • the reaction product may be detected by a characteristic of the product ' , such as color intensity, optical density, reflectance density, pH, fluorescence, or conductivity. For example, it may be detected by external visual inspection of the means defining a reaction zone. A contrast region surrounding said reaction zone aids detection by contrasting with the reaction zone in respect to the characteristic detected. An intensity scale may be included to aid quantative detection of sample analyte.
  • the reaction zone is contained in a test head, and the absorbent reservoir is concentrically positioned with respect to the test head, allowing telescoping movement of the absorbent reservoir with respect to the test head.
  • the test head includes at least one control region and at least one reaction zone, with dividers isolating each control region and each reaction zone.
  • the test head comprises an aqueous impermeable face plate having at least one opening to allow liquid to reach the reaction zone, and a second opening to allow liquid to read the control zone.
  • the test device is accompanied by a reagent pack sized and configured to supply a plurality of reagents to the reaction zone.
  • the device includes " a developer selected to participate in generating a colored substance from a chromophore.
  • the invention features a method for detecting an analyte in a sample by reacting the analyte with at least one liquid reagent to form a detectable reaction product.
  • the sample is contacted with the reaction zone, thereby causing the predetermined limited sample volume to flow through the means defining the reaction zone.
  • the absorbent reservoir is retained in the first position, the sample is contacted with the reaction zone, thereby flowing the predetermined limited sample volume through the means defining the reaction zone; thereafter, the absorbent reservoir is moved in contact with the control absorbent; while the absorbent reservoir is maintained in contact with.the control absorbent, the reaction zone is contacted with at least one of the liquid reagents, which flows through the reaction zone into the absorbent reservoir.
  • the reaction zone While the absorbent reservoir is maintained in the second position, the reaction zone may be contacted with one or more additional reagents which are allowed to flow through .the reaction zone into the absorbent reservoir. A predetermined time is allowed after the sample absorption and before moving the absorbent reservoir into the second position, to allow the analyte to react with reactant in the reaction zone. After the detectable product is formed, the absorbent reservoir may be returned to the first position to enable detection of the reaction product while the absorbent reservoir is in the first position, i.e., in the absence of continued flow through the reaction zone caused by the absorbent reservoir.
  • the absorbent reservoir can be maintained in the first position for a predetermined length of time, selected to control the desired reaction, so that both the volume of sample contacted with the reaction zone and the duration of that contact are controlled.
  • the test head of the device is at one end and is positioned downwardly to be immersed in the sample and the liquid reagent(s). Then, after the detectable product is formed, the device is inverted to position the test head on top during detection of the product.
  • the invention involves a particularly rapid assay that is easy to use with a minimal sample volume. The test result is reliably read, with enhanced background contrast and with reliable controls.
  • the test device is readily assembled with protection against error during assembly and use. The overall assay generally represents a significant cost savings.
  • Fig. 1 is a view of an immunoassay kit
  • Fig. 2 is an exploded view of the dipstick of
  • Fig. 2a is a view of the cap and barrel of the dipstick of Fig. 2, with other parts of the dipstick omitted.
  • Fig. 3 is a view, with parts broken away, showing assembly of the dipstick of Fig. 2;
  • Fig. 4 is a side view of the dipstick of Fig. 2. in section, with parts omitted for clarity, taken along 4-4 of Fig. 1;
  • Fig. 5 is an exploded cross-section of the dipstick of Fig. 2 taken along 5-5 of Fig. 2a, with parts omitted for clarity;
  • Fig. 6 is a view along 6-6 of Fig. 4;
  • Figs. 7A and 7B show, respectively, the two operating positions of the dipstick of Fig. 2;
  • Fig. 8 shows, in highly diagrammatic fashion, five steps in an assay using the kit of Fig. l;
  • Fig. 9 depicts a key for reading the results of the assay of Fig. 8.
  • an immunoassay kit 10 includes a dipstick 12 and a reagent tray 14 which has a clear lid 16. There are four wells 18a-18d in tray 14, described in greater detail below. Tray 14 also includes an elongated slot 15 to accomodate dipstick 12 and a circular sample well 19 to receive the sample.
  • dipstick 12 includes a test head 20, a barrel 24, and a cap 40.
  • Test head 20 (best shown in Fig. 6) is sized to be immersed in sample well 19 and in wells 18a-18d.
  • Test head 20 has three liquid-receiving openings in face 22. Round openings 21a and 21b are positive and negative control openings, respectively, as explained below. Opening 23 is the analyte detection opening.
  • An index notch 25 is positioned opposite positive control opening 21a to indicate the proper test head orientation when reading test results.
  • barrel 24 of the dipstick includes three axially extending fins 35. evenly spaced around the circumference of the interior of the barrel and integral with the barrel. As shown in Figs. 4 and 5, fins 35 extend to the head region of the dipstick, where they terminate in radial fins.26, which are shown in more detail in Figs. 2 and 5. Fins 26 are shaped and positioned to fit within three radial recesses 28 in the primary (or control) absorbent 30, described below, and each terminates in a sharp edge 27, designed to pierce depth matrix 32 during assembly.
  • a cylindrical absorbent reservoir 38, positioned within barrel 24, is described in greater detail below.
  • Axial ribs 35 center the absorbent reservoir.
  • Barrel 24 also includes an indexing block 52 extending radially beyond the circumference of the barrel.
  • Depth matrix 32 can be a glass fiber membrane (e.g., Gelman A/E; Pall 0-10) which is capable of absorbing microparticles as described below.
  • Control absorbent 30 is hydrophilic polyethylene material, which has slots 28 molded in it. For each test, a desired optimum sample volume is determined, and the control absorbent: depth and porosity are selected accordingly.
  • a typical volume of sample desired to be moved through the reaction zone is less than 4Q0 ⁇ l and certainly less than 1 ml (most preferably less than 150-200 ⁇ l).
  • a suitable control ' absorbent for a volume of 100-150 ⁇ l is a porous polyethylene (average pore size 40 ⁇ ) with a diameter of 0.410 inches and a thickness of 0.100 inches. Such material is available from Porex Technology, Fairburn GA or Chromex Inc., Brooklyn, New York.
  • the absorbent reservoir 38 comprises drawn cellulose acetate fibers of 3.5 to 4.5 denure.
  • the fibers may be oriented parallel to the barrel axis (along the direction of fluid movement) to provide fast wicking and thereby reduce the total assay time.
  • the fibers are treated with a plasticizer such as triacten to stiffen them and improve flow.
  • the absorbent reservoir should have excess capacity for the total liquid volume to be moved through the reaction zone, e.g., at least about 5 ml.
  • Cap 40 includes cross-members 46 and offset ribs 43 (Fig. 5) to pinch and retain absorbent reservoir 38, as shown in Fig. 4.
  • Indexing void 50 on the interior of cap 40 mates with indexing block 52 on the exterior of barrel 24.
  • a ridge 47 extends around the circumference of the interior of cap 40 and mates with circumferential ridges 44 and 54 on the exterior of barrel 24. Assembly
  • Solid portions of the dipstick are suitable plastic such as injection molded polypropylene.
  • the barrel and test head are molded as separate parts.
  • Reservoir 38 is pinched between cross-members 46 and ribs 43, so that the reservoir is retained away from control absorbent 30..
  • Cap 40 is forced over the end of barrel 24, with indexing void 50 positioned over indexing block 52.
  • Ridge 47 is snapped over ridge 44, but not over ridge 54, so absorbent reservoir 38 is maintained apart from control absorbent 30 as shown in position A of Fig. 7.
  • microparticles are spotted through test head ports into the assay region.
  • latex microparticles with anti-analyte antibody can be spotted through analyte detection port 23 onto depth matrix 32.
  • Latex particles containing the chromagen label e.g., the enzyme
  • positive control 21a Particles non-reactive to the sample or the reagents are spotted through negative control 21b.
  • the latex microparticles can be made of polystyrene. Proteins are immobilized on the particles by known techniques. See, e.g., Bangs, Uniform Latex Particles, Seragen Diagnostics.
  • the particles are suspended in a buffer (e.g., 0.5%-1.0% weight loading in a standard saline buffer appropriate for the enzyme at issue) to be dropped onto the reaction zone.
  • a buffer e.g. 0.5%-1.0% weight loading in a standard saline buffer appropriate for the enzyme at issue
  • kit 10 with dipstick 12 nested in slot 15, and lid 16 covering tray 14. After removing the lid, sample is added to well 19 according to a protocol that will depend upon the sample and the precise nature of the assay.
  • Dipstick 12 is removed from the slot.
  • the cap 40 is retracted, and the absorbent reservoir 38 is spaced apart from the control absorbent (position A in Fig. 7).
  • the test head 20 is immersed in sample well 19 (Fig. 8, step 1).
  • the amount of sample drawn through the reaction zone 32 is determined by control absorbent 30.
  • control absorbent 30 Specifically, the porosity and dimensions of the control absorbent control the sample volume drawn into the reaction zone.
  • the capacity of control absorbent 30 is limited—i.e., the control absorbent will not absorb all of the sample in well 19. Rather, once absorbent 30 is filled to capacity, sample flow through the reaction zone (i.e., from the receiving face of the zone to the outlet face of the zone) ceases.
  • control absorbent 30 meters the extent of flow of sample through the reaction zone predetermined (experimentally) to provide reliable analyte detection. That flow will depend on the amount of reagent available in the reaction zone, the intensity of the parameter being detected, the level of analyte as to which discrimination is desired, sample viscosity, and the sample volume available.
  • Example Anti-viral antibody, conjugated to latex microparticles by standard techniques are spotted through analyte detection port 23 onto the depth matrix 32. Similar latex microparticles containing enzyme are spotted through positive control port 21a onto assay region 32. Paticles are spotted through negative control port 21b.
  • Reagent wells are filled as follows:
  • 18a antibody-enzyme conjugate (30 sec. - 2 minutes. )
  • 18b wash solution (60 sec.)
  • 18c enzyme substrate (30 - 60 sec.)
  • stop solution A solution which either stops enzyme activity, and/or precipitates the substrate by changes in pH, ionic strength, or the addition of an inhibitor.
  • Suitable systems include well known alkaline phosphatase systems and horseradish peroxidas H-0- systems.
  • Each well 18a-18d contains a slight excess of the reagent in question, absorbed within sponge matrices 56a-56d which release a reagent when compacted by the test head. In this way, reagent loss through evaporation in shipping and storage is avoided.
  • test head in the test head can be a separate piece from the barrel.
  • the control absorbent and depth matrix can be manufactured as a single element.
  • an absorbent porous polyethylene substrate can be constructed to have constricted pores on the top surface using a cellulosic material.
  • the test tray can be packaged in a shrink-wrap plastic film, omitting the cover.
  • the test tray can be modified by changing the spacing and location of the wells. For example, sample well 19 can be moved to one end of the dipstick slot 15 and wells 18a-18d can be spaced apart, with a groove included between each well to contain liquid that drips and avoid contaminating the wells.
  • a range of immunoassay techniques are performed with the device, including radioactive and fluorescent techniques as well as the colorimetric technique described in this patent application. The size of the device can be adjusted depending on assay volume.
  • a pre-filter can be used to remove undesired elements of the sample that hinder the assay, e.g. red blood cells in a whole blood assay.
  • the number of openings or ports in the test head can be increased, e.g. , multiple assays can be performed on the same sample, by spotting different analyte binding agents in different openings.
  • the test can be read by automated reading apparatus, as well as by visual inspection.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
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  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Plasma & Fusion (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

Un dispositif d'essai (12) pour détecter un analyte dans un échantillon liquide en traitant celui-ci avec des réactifs liquides pour former un produit de réaction détectable. Le dispositif (12) comprend une zone de réaction (32) capable de retenir le produit de réaction détectable et un absorbant de contrôle (30) placé en contact de transfert de liquide avec la zone de réaction (32). L'absorbant de contrôle (30) possède une capacité prédéterminée d'absorption de liquide et est placé de manière à ce que, lorsque l'absorbant de contrôle (30) est rempli à pleine capacité, il mesure en fait un débit prédéterminé à travers la zone de réaction (32). Le dispositif comprend également (avec ou sans l'absorbant de contrôle (30)): un réservoir d'absorbant (38); un moyen pour retenir celui-ci (38) dans une première position éloignée de l'absorbant de contrôle (30), de façon à empêcher un transfert de liquide; et un moyen pour déplacer le réservoir d'absorbant (38) d'une première position à une deuxième position permettant un transfert de liquide avec l'absorbant de contrôle (30).
PCT/US1988/004059 1988-11-14 1988-11-14 Detection mixte d'analyte a l'aide d'un absorbant WO1990005906A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/US1988/004059 WO1990005906A1 (fr) 1988-11-14 1988-11-14 Detection mixte d'analyte a l'aide d'un absorbant
EP19890902484 EP0442872A4 (en) 1988-11-14 1988-11-14 Dual absorbent analyte detection
JP50236589A JPH04502961A (ja) 1988-11-14 1988-11-14 二重吸収被分析物検出

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1988/004059 WO1990005906A1 (fr) 1988-11-14 1988-11-14 Detection mixte d'analyte a l'aide d'un absorbant

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WO1990005906A1 true WO1990005906A1 (fr) 1990-05-31

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JP (1) JPH04502961A (fr)
WO (1) WO1990005906A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0651249A2 (fr) * 1993-11-03 1995-05-03 Cogent Diagnostics Limited Appareil analytique
AU665956B2 (en) * 1991-05-29 1996-01-25 Beckman Coulter, Inc. Assay device
GB2521119A (en) * 2013-10-30 2015-06-17 Surescreen Diagnostics Ltd Indication device
US9656264B2 (en) 2011-02-28 2017-05-23 Ge Healthcare Uk Limited Biological sample holder and method of assembling same

Citations (7)

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US3888629A (en) * 1971-09-08 1975-06-10 Kenneth Dawson Bagshawe Performance of chemical or biological reactions within an absorbent matrix pad
US4246339A (en) * 1978-11-01 1981-01-20 Millipore Corporation Test device
US4366241A (en) * 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4632901A (en) * 1984-05-11 1986-12-30 Hybritech Incorporated Method and apparatus for immunoassays
US4717656A (en) * 1983-12-02 1988-01-05 Vertrik Bioteknik Ab Device for chemical analyses and use thereof
EP0253579A1 (fr) * 1986-07-14 1988-01-20 Hybritech Incorporated Appareil et procédé pour essai immunologique
US4797260A (en) * 1987-01-27 1989-01-10 V-Tech, Inc. Antibody testing system

Family Cites Families (4)

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AU665956B2 (en) * 1991-05-29 1996-01-25 Beckman Coulter, Inc. Assay device
EP0651249A2 (fr) * 1993-11-03 1995-05-03 Cogent Diagnostics Limited Appareil analytique
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US9656264B2 (en) 2011-02-28 2017-05-23 Ge Healthcare Uk Limited Biological sample holder and method of assembling same
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Also Published As

Publication number Publication date
EP0442872A4 (en) 1992-08-05
JPH04502961A (ja) 1992-05-28
EP0442872A1 (fr) 1991-08-28

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