WO1990005779A1 - Selection sexuelle de la mouche mediterraneenne a ailes defective - Google Patents
Selection sexuelle de la mouche mediterraneenne a ailes defective Download PDFInfo
- Publication number
- WO1990005779A1 WO1990005779A1 PCT/US1989/004728 US8904728W WO9005779A1 WO 1990005779 A1 WO1990005779 A1 WO 1990005779A1 US 8904728 W US8904728 W US 8904728W WO 9005779 A1 WO9005779 A1 WO 9005779A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- wing
- phenotype
- wild
- medflies
- medfly
- Prior art date
Links
- 241000255579 Ceratitis capitata Species 0.000 title claims abstract description 47
- 230000002950 deficient Effects 0.000 title description 2
- 230000005945 translocation Effects 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 21
- 230000013011 mating Effects 0.000 claims description 14
- 230000014509 gene expression Effects 0.000 claims description 6
- 108700028369 Alleles Proteins 0.000 claims description 4
- 206010061217 Infestation Diseases 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 abstract 1
- 241000255925 Diptera Species 0.000 description 14
- 230000002068 genetic effect Effects 0.000 description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 210000002593 Y chromosome Anatomy 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 210000002257 embryonic structure Anatomy 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 229930195722 L-methionine Natural products 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 108010079058 casein hydrolysate Proteins 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000032669 eclosion Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 229960004452 methionine Drugs 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 208000034951 Genetic Translocation Diseases 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 108700005090 Lethal Genes Proteins 0.000 description 1
- 239000005949 Malathion Substances 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 241000006364 Torula Species 0.000 description 1
- 210000001766 X chromosome Anatomy 0.000 description 1
- 108700029634 Y-Linked Genes Proteins 0.000 description 1
- 208000028258 Y-linked inheritance Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 239000002254 cytotoxic agent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 229960000453 malathion Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- MBABOKRGFJTBAE-UHFFFAOYSA-N methyl methanesulfonate Chemical compound COS(C)(=O)=O MBABOKRGFJTBAE-UHFFFAOYSA-N 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 208000016021 phenotype Diseases 0.000 description 1
- VVWWGULTERRQST-UHFFFAOYSA-M potassium;phosphoric acid;chloride Chemical compound [Cl-].[K+].OP(O)(O)=O VVWWGULTERRQST-UHFFFAOYSA-M 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Definitions
- the subject invention concerns production of sterilized male medflies free of female medflies using genetic lesions for separation.
- the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann), is a major agriculture pest in temperate and sub-tropical regions worldwide. The medfly attacks over 150 varieties of cultivated fruit crops. It is found throughout the world in a variety of climes. Current control practice in most areas consist of the use of bait sprays, i.e., an attractant such as yeast hydrolysate mixed with an insecticide such as malathion. In addition, the United States Department of Agriculture has used the sterile insect release method (SIRM) in California and Texas and has participated in or consulted on SIRM in Central America and other places throughout the world.
- SIRM sterile insect release method
- the SIRM would have greater world wide use if not for several current and potential problems. Released females, though sterile, will sting or puncture fruit while attempting to oviposit, allowing the entrance of decay organisms and causing considerable, sometimes severe, damage. The large number of sterile females released in conjunction with sterile males, where there is no separation, can monopolize the matings with the released males needed for the sterile control of wild fertile females. There is evidence that sterile females mate with sterile males (intrastrain mating) more readily than with wild males (interstrain mating). Since released females will outnumber wild females up to a 50 to 1 ratio, they may be involved in the vast majority of matings by released males. This decrease in the likelihood of matings between sterile males and native fertile females greatly reduces the efficiency of SIRM.
- a genetic sexing method would not only help avoid the problems described above, but could add several distinct benefits to a SIRM program. Sterilization technologies could be fined tuned to maximize sterile males without regard for effects on females, so that male competitiveness might be easier to improve.
- a genetic marker could be provided in some programs that would identify inadvertent releases of fertile males as a result of accidental failure to sterilize and reduce opportunities for unexplained failures of some programs.
- Ceratitis capitata (Medfly) and the resulting medflies are provided for controlling the medfly population in an environment subject to infestation.
- the method involves providing breeding stocks of male and female medflies, where the males have the normal wing pheno ⁇ type and include an autosomal Y-translocation involving one arm of the autosome containing the ap_ and d_c genes with the Y-chromosome, where the wild-type gene allele of the v-wing mutated gene phenotype is involved in a translocation with the Y-chromosome.
- the normal wing phenotype male containing the Y-chromosomal translocation is mated with homozygous v-wing females.
- Lines may then be propagated and bred through a plurality of generations, where the progeny contain only normal wing phenotype males and v-wing phenotype females.
- the progeny containing medflies of both sexes may then be sterilized in accordance with conventional ways, for example, gamma radiation employing a total dosage of least about 10, usually 15Krads.
- the v-wing mutant may be obtained in a variety of ways. Particularly, routine screening of laboratory stocks can uncover the v-wing mutant.
- fly stocks can be mutagenized by standard techniques, which include but are not limited by the following: Medflies may be irradiated with dosages in the range of about 2 to 8 Krad to induce mutations, ' followed by mating and screening the progeny for the v-wing phenotype.
- Larvae may be subjected to a wide variety of mutagens, such as nitrosoguanidine, methyl methanesulfonate, formalin, or the like and the resulting medflies are screened for the v-wing phenotype.
- embryos may be transformed with P-type transposable elements, which include a selective marker.
- Introduction of the P-type element into the embryos can be achieved by injection, employing a potassium chloride phosphate-buffered solution having from about 50 to 500 ⁇ g/ml of P-type element DNA.
- the embryos which contain the P-type element are detected by resistance to a selective medium.
- the resistance may be to a cytotoxic agent, such as an antibiotic, methotrexate, heavy metal or other toxin.
- P-type transposable elements are available for use with some diptera. By transforming a sufficient number of embryos, one may then screen the progeny for the v-wing mutation.
- DNA from translocation stock and from homozygous v-wing stock is isolated by the method described by Poustka and Lehrach, Trends in Genetics (1986) 2:174-179.
- the DNA is cloned into a large insert 40kb cosmid library.
- the clones are probed with total Y-chromosome Medfly DNA isolated by a fluorescence activated cell sorter based on the fluorescence of the Y-chromosome.
- the library is reprobed with total genomic Medfly DNA or DNA of the X-chromosome plus the autosome containing the wild-type wing gene complementary to the v-wing mutant, which can be obtained by fluorescence activated cell sorting.
- Clones which hybridize to both probes contain the translocation junction points.
- the v-wing mutant autosome chromosome is walked down from the junction towards the v-wing gene using techniques descsribed in Poustka and Lehrach, supra. Clones obtained in the walking procedure are used to transform wild-type flies. Clones which are specific for the v-wing mutant medfly are transformed into wild-type larvae in accordance with the procedure described in Rubin and Spaulding, Nucleic Acids Res. (1983) 11:6341-6351. See also, Thomas et al.. Nature (1986) 324:34-38; Song et al., Proc. Natl. Acad. Sci. (1987) 84: 6820-6824; and U.
- the v-wing phenotype may be variable at one temperature and uniformly extreme at a different temperature.
- the v-wing mutant employed in the subject invention is found to provide about 10 to 20% of medflies with stubby remnants when reared at room temperature, while 100% of the medflies have stubs and are totally unable to fly when reared at 30°C.
- the larvae may be grown in any conventional larval food recipe.
- the conventional nutrient media will usually include a base of wheat bran plus sucrose, yeast r a vitamin fortification, as well as other additives.
- Adults may be raised on sucrose, yeast hydrolysate, casein hydrolysate, and as appropriate, essential amino acids, e.g., L-methionine.
- the pupae are irradiated to sterility one day before adult eclosion. Sterility can be achieved by irradiation at a total dose of up to about 18Krads of gamma irradiation in a nitrogen atmosphere.
- the resulting sterile fruit flies may then be introduced into the environment to be protected, in accordance with conventional methods.
- the flies may be placed in an open container, where the females are incapable of flying over the walls of the container, while the males may freely leave the container.
- the sterile males may then compete with wild-type males for the fertile females to substantially diminish the medfly population.
- the v-wing stock was produced from a single female fly found in routine screening of laboratory stocks. After a true breeding stock was produced, crosses revealed that this apparently spontaneous mutant is an autosomal recessive located on the same chromosome as ap and dc genes.
- Homozygous v-wing flies reared at room temperature show a wide range of phenotypic expressions from a few percent of flies with essentially wild-type wings to 10-20% with only stubby remnants. At 30°C 100% of the flies have only stubs and are totally unable to fly.
- Larval food consisted of 250 g sucrose, 250 g torula yeast, 500 g of wheat bran and 10 g of Vanderzant vitamin fortification for insects (U.S. Biochemical Co.), 1 L distilled water containing 15 ml of cone. HC1 and 10 ml of a solution containing 750 mg of methylparaben and 1 g of ascorbic acid dissolved in 95% ethanol.
- Translocations were produced by irradiating 24-48 hr old male flies in air with a dose of 4800 rads in the Hawaii Research Irradiator fcobalt-60 source). The mating scheme to isolate Y-autosome translocations then followed the plan used by Saul (1984). After irradiation the males were held for 1 day and then mass mated to virgin v-wing females. Each male offspring from this cross was pair mated to several female v-wing flies. Those crosses which gave progeny suggestive of a desired translocation, i.e., all wild type males and all v-wing females, were saved and propagated. One line (#28) from the first 102 male lines has proven to have just such a Y-autosome translocation exhibiting Y- linked inheritance of the wild-type V-wing allele. Additional lines are still being investigated.
- a genetic sexing method for medflies' where females are produced having a physical deficiency which allows for the automatic separation of females from males and prohibits the females from affecting fruit or large scale mating with the sterile males.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Des mouches méditerranéennes ou Ceratitis capitata mâles stérilisées, à l'exclusion des femelles, sont introduites dans un environnement en établissant une lignée dans laquelle les femelles ont un phénotype d'ailes non fonctionnelles à des températures élevées et les mâles ont une translocation à autosome Y avec le gène de type sauvage associé au phénotype alaire. En croisant les deux et en stérilisant les descendants et en libérant les pupes ou les adultes dans l'environnement, les mâles sont libres de voler et de s'accoupler avec les mouches méditerranéennes femelles sauvages, tandis que les femelles ailées non fonctionnelles sont retenues au niveau du site de libération.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27498988A | 1988-11-22 | 1988-11-22 | |
| US274,989 | 1988-11-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1990005779A1 true WO1990005779A1 (fr) | 1990-05-31 |
Family
ID=23050441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1989/004728 WO1990005779A1 (fr) | 1988-11-22 | 1989-10-20 | Selection sexuelle de la mouche mediterraneenne a ailes defective |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0404890A4 (fr) |
| AU (1) | AU4635289A (fr) |
| IL (1) | IL92290A0 (fr) |
| PT (1) | PT92371A (fr) |
| WO (1) | WO1990005779A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009067089A1 (fr) * | 2007-11-21 | 2009-05-28 | Wong Ching Sing | Système automatisé d'élevage d'insectes |
| CN115088681A (zh) * | 2022-08-02 | 2022-09-23 | 浙江省农业科学院 | 一种获得番茄潜叶蛾不育雄虫的方法及其在该虫防治中的应用 |
-
1989
- 1989-10-20 AU AU46352/89A patent/AU4635289A/en not_active Abandoned
- 1989-10-20 WO PCT/US1989/004728 patent/WO1990005779A1/fr not_active Application Discontinuation
- 1989-10-20 EP EP19890913040 patent/EP0404890A4/en not_active Withdrawn
- 1989-11-13 IL IL92290A patent/IL92290A0/xx unknown
- 1989-11-22 PT PT92371A patent/PT92371A/pt not_active Application Discontinuation
Non-Patent Citations (12)
| Title |
|---|
| Annals of the Entomological Society of America, Volume 75, issued 1982, S.H. SAUL, "Rosy-Like Mutant of the Mediterranean Fruit Fly, Ceratitis Capitata (Diptera: Tephritidae), and its Potential for use in a Genetic Sexing Program", 480-483. * |
| Annals of the Entomological Society of America, Volume 77, issued 1984, S.H. SAUL, "Genetic Sexing in the Mediterranean Fruit Fly, Ceratitis Capitata (WEIDEMANN) (Diptera: Tephritidae): Conditional Lethal Translocations that Preferentially Eliminate Females", pages 280-283. * |
| Argicultural Zoology Reviews, Volume 1 issued 1986, S.H. SAUL, "Genetics of the Mediterranean Fruit Fly (Ceratitis Capitata) (WEIDEMANN)", pages 73-108. * |
| Biological Abstracts, Volume 72, issued 1981, BOWNES et al., "Regulative Properties of Wing Discs from the Vestigial (vg) Mutant of Drosophila Melanogaster", Abstract no. 51916. * |
| EMBO Journal, Volume 7, issued 1988, WILLIAMS et al., "Molecular Organization of the Vestigial Region in Drosophila Melanogaster, pages 1355-1363. * |
| Entomophaga Volume 24, issued 1979, Y. ROSSLER, "Automated Sexing of Ceratitis Capitata (Dip.: Tephritidae) The Development of Strains with Inherited Sex-Limited Pupal Color Dimorphism" pages 411-416. * |
| Genetica, Volume 58, issued 1982, A.S. ROBINSON et al., "Ceratitis Capitatasuitable Case for Genetic Sexing", pages 229-237. * |
| Genetica, Volume 62, issued 1984, A.H. ROBINSON, "Unexpected Segregation Ratios from Male-Linked Translocations in the Mediterranean Fruit Fly Ceratitis Capitata (Diptera: Tephritidae)", pages 209-215. * |
| Journal of Economical Entomology, Volume 74, issued 1981, OZAKI et al., "Effects of Pupal Handling during Laboratory Rearing on Adult Eclosion and Flight Cability in Three Tephritid Species", pages 520-525. * |
| Science, Volume 172, issued 1971, I.C. McDONALD, "A Male-Producing Strains of the House Fly, page 489. * |
| See also references of EP0404890A4 * |
| Theoretical and Applied Genetics, Volume 59, issued 1981, A.S. ROBINSON et al., "Genetic Sexing in Drosophila Melanogaster using Alcohol Dehydrogenase Locus and a Y-Linked Translocation", pages 23-24 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009067089A1 (fr) * | 2007-11-21 | 2009-05-28 | Wong Ching Sing | Système automatisé d'élevage d'insectes |
| CN115088681A (zh) * | 2022-08-02 | 2022-09-23 | 浙江省农业科学院 | 一种获得番茄潜叶蛾不育雄虫的方法及其在该虫防治中的应用 |
| CN115088681B (zh) * | 2022-08-02 | 2024-03-08 | 浙江省农业科学院 | 一种获得番茄潜叶蛾不育雄虫的方法及其在该虫防治中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU4635289A (en) | 1990-06-12 |
| PT92371A (pt) | 1990-05-31 |
| EP0404890A4 (en) | 1992-01-08 |
| IL92290A0 (en) | 1990-07-26 |
| EP0404890A1 (fr) | 1991-01-02 |
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