WO1990003441A1 - Determination de la sensibilite de bacteries vis a vis d'anticorps, par microdilution acceleree - Google Patents

Determination de la sensibilite de bacteries vis a vis d'anticorps, par microdilution acceleree Download PDF

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Publication number
WO1990003441A1
WO1990003441A1 PCT/US1989/003971 US8903971W WO9003441A1 WO 1990003441 A1 WO1990003441 A1 WO 1990003441A1 US 8903971 W US8903971 W US 8903971W WO 9003441 A1 WO9003441 A1 WO 9003441A1
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WO
WIPO (PCT)
Prior art keywords
aliquots
oxygen
incubation
antibiotic
aliquot
Prior art date
Application number
PCT/US1989/003971
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English (en)
Inventor
Marvin Murray
Original Assignee
Marvin Murray
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Marvin Murray filed Critical Marvin Murray
Publication of WO1990003441A1 publication Critical patent/WO1990003441A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material

Definitions

  • This invention relates to the testing of bacteria to determine their susceptibility to specif ⁇ ic antibiotics.
  • the invention particularly relates to the microdilution method of determining antibiotic susceptibility in which a minimum inhibitory concen ⁇ tration (MIC) of the antibiotic to a bacterial pathogen can be determined.
  • MIC minimum inhibitory concen ⁇ tration
  • microdilution test procedures More quantitative results are obtained by microdilution test procedures.
  • a series of samples are prepared in microdilution trays containing the bacterium at a standard concentration and progressive dilutions of the antibiotic being tested.
  • samples are incubated overnight and usually for about 16 to 18 hours.
  • determinations can sometimes be made in a short ⁇ er time but at least 6 to 8 hours is required.
  • the extent of bacterial growth or inhibition is deter ⁇ mined by measuring the turbidity of the samples. The endpoint is taken at the lowest concentration of the antimicrobi ⁇ at which the microorganism tested does not show growth. Aerobic bacteria are known to respire.
  • test samples were inoculated with bacterial cells still in their lag growth phase.
  • the samples were aerated to the same oxygen partial pressure (p0 2 ), and cultured under conditions promoting a rapid transition from lag phase to exponential growth phase. Measurements of p ⁇ 2 were made during the transitional period, viz., either at the end of the lag phase or the beginning of the growth phase.
  • This invention provides a method for acceler ⁇ ated determination of antimicrobial susceptibility of oxyphilic bacteria.
  • the bacteria are oxy- philic pathogens, classified as “aerobic” or “micro- aerophilic”.
  • a pure culture of the bacteria to be tested is obtained. This can be done by standard methods of isolation, as described for example in the Manual of Clinical Microbiology, Lennette, Editor, 3rd ed. , 1980, 68-71.
  • the prepared aliquots are then subjected to a preparatory incubation for a time sufficient for the antibiotic to act on the bacterial cells.
  • a preparatory incubation for a time sufficient for the antibiotic to act on the bacterial cells.
  • the oxygen in all of the ali ⁇ quots can be substantially depleted.
  • preincubation times ranging from 60 to 120 minutes.
  • a presently preferred preincubation time is from about 60 to 130 minutes, or optimally around 90 minutes.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Dans un procédé de microdilution accélérée permettant de déterminer la sensibilité d'une bactérie oxyphilique à un antibiotique, on soumet un aliquot de régulation positive contenant la bactérie sans l'antibiotique ainsi que les aliquots de test contenant la bactérie et augmentant les concentrations de l'antibiotique, à une incubation préparatoire pendant un moment suffisant pour que l'antibiotique agisse sur les cellules bactériennes et utilise l'oxygène se trouvant dans celles-ci. On ajoute une quantité prédéterminée d'oxygène aux aliquots, on procède à une seconde incubation jusqu'à ce que la teneur en oxygène de l'aliquot de régulation positive soit réduite d'au moins 50 %, puis on détermine quantitativement la teneur en oxygène de la série servant de mesure de la sensibilité à un antibiotique.
PCT/US1989/003971 1988-09-20 1989-09-12 Determination de la sensibilite de bacteries vis a vis d'anticorps, par microdilution acceleree WO1990003441A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24680888A 1988-09-20 1988-09-20
US246,808 1988-09-20

Publications (1)

Publication Number Publication Date
WO1990003441A1 true WO1990003441A1 (fr) 1990-04-05

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/003971 WO1990003441A1 (fr) 1988-09-20 1989-09-12 Determination de la sensibilite de bacteries vis a vis d'anticorps, par microdilution acceleree

Country Status (2)

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AU (1) AU4338489A (fr)
WO (1) WO1990003441A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0632131A1 (fr) * 1992-07-22 1995-01-04 Daikin Industries, Limited Procede et appareil d'examen recourant a un agent antibacterien
US5876959A (en) * 1992-07-22 1999-03-02 Daikin Industries, Ltd. Method for testing infectious disease causing microorganisms
US6143555A (en) * 1992-07-22 2000-11-07 Daikin Industries Ltd. Infectious disease inspection method and apparatus therefor
US6251405B1 (en) 1995-06-07 2001-06-26 Connaught Laboratories, Inc. Immunological combination compositions and methods
WO2019108125A1 (fr) * 2017-11-30 2019-06-06 Ascilion Ab Procédé de détermination de la sensibilité de microbes à des agents antibiotiques

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2747567A1 (de) * 1976-10-25 1978-04-27 British Petroleum Co Verfahren zur bestimmung der toxizitaet von industrieabwaessern
DE2651896A1 (de) * 1976-11-13 1978-05-18 Bbc Brown Boveri & Cie Verfahren zum bestimmen der toxizitaet von waessrigen fluessigkeiten
DE2752356A1 (de) * 1976-11-24 1978-06-01 Haus Der Gesundheit Verfahren zur pruefung der chemotherapeutischen empfindlichkeit von mikroorganismen
US4162195A (en) * 1974-04-04 1979-07-24 Aktiebolaget Kalle-Regulatorer Biological testing device and method of measuring toxicity of sewage
EP0006376A1 (fr) * 1978-06-15 1980-01-09 Rhone-Poulenc Industries Procédé de mesure et/ou de détection de toxicité et appareillage pour ledit procédé
EP0128527A2 (fr) * 1983-06-09 1984-12-19 Marvin Murray Détermination accélérée de la susceptibilité antimicrobienne de bactéries
JPS60110300A (ja) * 1983-11-16 1985-06-15 Chiyo Masuda 抗菌製剤の経時的抗菌力測定法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4162195A (en) * 1974-04-04 1979-07-24 Aktiebolaget Kalle-Regulatorer Biological testing device and method of measuring toxicity of sewage
DE2747567A1 (de) * 1976-10-25 1978-04-27 British Petroleum Co Verfahren zur bestimmung der toxizitaet von industrieabwaessern
DE2651896A1 (de) * 1976-11-13 1978-05-18 Bbc Brown Boveri & Cie Verfahren zum bestimmen der toxizitaet von waessrigen fluessigkeiten
DE2752356A1 (de) * 1976-11-24 1978-06-01 Haus Der Gesundheit Verfahren zur pruefung der chemotherapeutischen empfindlichkeit von mikroorganismen
EP0006376A1 (fr) * 1978-06-15 1980-01-09 Rhone-Poulenc Industries Procédé de mesure et/ou de détection de toxicité et appareillage pour ledit procédé
EP0128527A2 (fr) * 1983-06-09 1984-12-19 Marvin Murray Détermination accélérée de la susceptibilité antimicrobienne de bactéries
JPS60110300A (ja) * 1983-11-16 1985-06-15 Chiyo Masuda 抗菌製剤の経時的抗菌力測定法

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0632131A1 (fr) * 1992-07-22 1995-01-04 Daikin Industries, Limited Procede et appareil d'examen recourant a un agent antibacterien
AU675444B2 (en) * 1992-07-22 1997-02-06 Daikin Industries, Ltd. Method of examining with antibacterial and apparatus therefor
EP0632131A4 (fr) * 1992-07-22 1997-04-16 Daikin Ind Ltd Procede et appareil d'examen recourant a un agent antibacterien.
US5876959A (en) * 1992-07-22 1999-03-02 Daikin Industries, Ltd. Method for testing infectious disease causing microorganisms
US6143555A (en) * 1992-07-22 2000-11-07 Daikin Industries Ltd. Infectious disease inspection method and apparatus therefor
US6251405B1 (en) 1995-06-07 2001-06-26 Connaught Laboratories, Inc. Immunological combination compositions and methods
US6379675B1 (en) 1995-06-07 2002-04-30 Connaught Laboratories, Inc. Immunological combination compositions and methods
WO2019108125A1 (fr) * 2017-11-30 2019-06-06 Ascilion Ab Procédé de détermination de la sensibilité de microbes à des agents antibiotiques

Also Published As

Publication number Publication date
AU4338489A (en) 1990-04-18

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