WO1989006788A1 - Procede et dispositif d'analyse cellulaire - Google Patents

Procede et dispositif d'analyse cellulaire Download PDF

Info

Publication number
WO1989006788A1
WO1989006788A1 PCT/US1989/000193 US8900193W WO8906788A1 WO 1989006788 A1 WO1989006788 A1 WO 1989006788A1 US 8900193 W US8900193 W US 8900193W WO 8906788 A1 WO8906788 A1 WO 8906788A1
Authority
WO
WIPO (PCT)
Prior art keywords
test sample
discrete
support
support means
predetermined pattern
Prior art date
Application number
PCT/US1989/000193
Other languages
English (en)
Inventor
Jonathan Maimon
Original Assignee
Long Island Jewish Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Long Island Jewish Medical Center filed Critical Long Island Jewish Medical Center
Priority to KR1019890701731A priority Critical patent/KR900700867A/ko
Publication of WO1989006788A1 publication Critical patent/WO1989006788A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L99/00—Subject matter not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices

Definitions

  • the invention is directed to a method and. apparatus, which may be in kit form, for accurate and rapid mass processing of large numbers of test samples absorbed onto an absorbent support, such as a nitrocellulose paper support, or other suitable support.
  • the apparatus of the invention is particularly well suited for rapid assaying of large numbers of experimental cell cultures grown in standard tissue culture plates having 96 individual culture wells arranged in 8 rows of 12 wells each.
  • Sensitive assay systems for screening cell culture test samples for the presence of a specific product which entail adsorption of a small quantity of a culture medium onto nitrocellulose paper, or other suitable adsorbent support means, and assay of the absorbed material in situ.
  • Immunoassay methods known in the art for assay of material absorbed on, e.g. , nitrocellulose supports include radioimmunoassay (RIA) and enzyme- linked-immunosorbent assay (ELISA) .
  • Still other known assays which are performed on test samples absorbed on a paper support include the use of labeled RNA and DNA probes to assay culture samples absorbed on nitrocellulose for the presence of a complimentary segment of DNA.
  • an immunoassay is performed whereby a radio-labelled antigen or an antigen linked with an enzyme is contacted with the test sample which is absorbed onto the "* support. Binding of the antigen to the absorbed test sample indicates the presence of the desired antibody product. Binding of the antigen can be conveniently determined in situ by a visible color reaction (ELISA) or by counting the radioactivity (RIA) that is bound to the test sample absorbed on the nitrocellulose, depending upon the type of immunoassay system used. Although these assay systems are reliable and easily performed, they are labor-intensive, and become quite burdensome and expensive when mass screening " of many test culture samples is involved.
  • a mouse myeloma cell is fused with a spleen cell of a mouse immunized with a specific antigen in order to obtain an individual cell line that is producing a myeloma antibody to that antigen in tissue culture.
  • myeloma cells Of those cells which fuse into immortal hybrid cells (“hybridomas") , only a small fraction produce an antibody to the desired antigen. It is therefore necessary to isolate from the heterogeneous population of experimental hybridomas, the one hybridoma cell which produces a monoclonal antibody to the specific antigen.
  • the experimental cell culture is distributed among a plurality of wells in tissue culture plates. Typically, these plates have 96 wells per plate.
  • the cells are incubated and divide in their individual wells, and a sample is taken from the culture medium in each well and then assayed for the presence of the specific desired antibody. If a sample from a well tests positive for the specific antibody, the cells are taken up and the population divided by redistribution into other plates of, e.g. , 96 wells.
  • the cells are incubated in these wells and the assay is repeated again for the detection of samples in wells containing cells producing the desired antibody. In this manner, antibody-positive wells are repeatedly identified and subcloned, until all the cells in a given well are derived from a single cell, i.e., the cell culture is monoclonal.
  • test culture sample must be taken from each well and absorbed onto an adsorbent assay support such as nitrocellulose paper, and each absorbed test sample spot must then be assayed by an RIA or ELISA procedure.
  • a small sample is withdrawn from each cell culture well and absorbed onto nitrocellulose paper.
  • This first application provides any antibodies that are being produced in the culture medium.
  • a blocking solution of BSA, gelatin or other solution is applied to block nonspecific protein binding by the paper. To do this, the entire sheet of nitrocellulose paper is submerged in the blocking solution.
  • the third step is the application of the specific antigen to the nitrocellulose paper having the cell culture sample absorbed thereon.
  • the antigen is usually conjugated to an enzyme which can be assayed by a color reaction. If there is an antibody present in the cell culture absorbed onto the nitrocellulose paper, the antibody binds the antigen and thereby also the conjugated enzyme.
  • the fourth step is the application of the 5 substrate for the conjugated enzyme.
  • a measurable color develops on the nitrocellulose paper where the test sample was absorbed.
  • a color reaction is visible on the nitrocellulose paper when the specific 10 antibody to the antigen conjugated to the enzyme is present in the absorbed cell culture test sample.
  • the intensity of the color is proportional to the amount of specific antibody in the cell culture.
  • labelled antigen is then applied to the nitrocellulose paper containing the absorbed test culture sample.
  • the radio-labelled antigen is bound to the test sample only if the specific antibody to the antige'n is present in the test sample. Radioactivity bound to the
  • nitrocellulose paper through the test sample is counted in a gamma or liquid scintillation counter.
  • the apparatus and method of the invention are particularly suited for rapid screening of experimental cell cultures, such as hybridomas or transformed cells, in which, in the absence of the invention, hundreds or thousands of individual samples of culture wells in tissue culture plates must be tediously screened for those few cells producing a desired bioproduct, as discussed above.
  • the invention may also be utilized to perform any assay of a test sample on an adsorbent support, such as nitrocellulose paper, as e.g. , isotopic assays, and nonisotopic assays including competitive or non-competitive enzyme-linked immunoabsorbent assays, fluorescent immunoassays, che iluminescent assays, bioluminescent assays, and the like.
  • a guide apparatus for simultaneously positioning and applying a number of discrete liquid assay test samples in a predetermined pattern (e.g. , the pattern of cell culture wells in a standard cell " culture plate) on an adsorbent assay support.
  • the invention comprises a guide and positioning means to simultaneously receive and position a means for dispensing a plurality of discrete test samples (e.g. , a twelve tip multichannel pipette) in said predetermined pattern; and means to receive, support and position a planar adsorbent assay support means (e.g. , a sheet of nitrocellulose paper) at a position which is vertically spaced and aligned below the guide and positioning means.
  • a plurality of discrete test samples may be simultaneously deposited on the planar absorbent assay support means to provide a plurality of discrete test sample spots absorbed on the assay support in accordance with the predetermined pattern.
  • the nitrocellulose paper or other assay support means has dimensions which match the dimensions of a standard (commercially available) tissue culture plate having 96 wells arranged in 8 rows of 12 wells each.
  • the paper is marked in a manner to indicate the orientation of the paper to correctly correspond to the 96 well cell culture plate.
  • the predetermined pattern of the guide and positioning means includes 96 dispensing means positions arranged in 8 rows of 12 positions, corresponding to the row configuration of the tissue culture plate.
  • the liquid dispensing means is preferably a 12-tip multi-channel pipette whose tips fit the dimensions of the 12 wells in a single row of the cell culture plate as well as the 12 guide positions of each row of the guide and positioning means.
  • the 12-tip multi-channel pipette is used to readily, simultaneously transfer discrete test samples, one from each of the wells of the cell culture plate, to corresponding positions on the guide and positioning means for deposition of discrete test samples onto the absorbent support positioned below the guide and positioning means.
  • the apparatus of the invention therefore provides an assay support template, such as a nitrocellulose assay template, with discrete test sample spots positioned to correspond to the positions of the 96 wells in a cell culture plate.
  • nitrocellulose assay templates may be rapidly prepared, one for each 96-well-cell-culture plate.
  • the nitrocellulose assay template with discrete absorbed culture samples is removed from the guide and positioning means and immersed into a bath container for simultaneous processing with other templates according to the immunoassay system chosen.
  • the bath container may accomodate, for example, as many as ten nitrocellulose templates in a removable template organizer and may be processed through a sequence of bath containers to perform a desired assay.
  • up to 960 assays of cell culture test samples may be readily processed simultaneously in one unit.
  • each of the nitrocellulose templates may be rapidly scanned for any color reaction if an ELISA system was used.
  • a multiple punch device for simultaneously detaching and separating the plurality of discrete test samples absorbed on the adsorbent assay support template in said predetermined pattern and delivering the detached test samples to individual receiver means arranged in accordance with the predetermined pattern.
  • the assay employed involves the binding of a radio-label to the absorbed test spots
  • the nitrocellulose assay template is placed in the multiple punch device for detaching and separating the plurality of test sample spots.
  • the multiple punch device punches out all the test spots at a single press and blows all the cut discs into the individual receiver means arranged in the predetermined pattern and suitable for use in a radiolabel counter.
  • Fig. 1 is a perspective view of a preferred embodiment of a guide and positioning means according to the invention for applying and positioning a plurality of liquid test samples on an absorbent assay support means.
  • Fig. 2 is a side cross-sectional view taken generally along line 2-2 of Fig. " 1.
  • Fig. 3 is a plan view of a preferred embodiment of a slide-like frame means according to the invention to hold the absorbent support means.
  • Fig. 4 is a perspective view of a template holder to accomodate a plurality of absorbent assay support means for simultaneous processing according to the invention.
  • Fig. 5 is a perspective view of a punch apparatus for detaching and separating a plurality of test sample spots absorbed on an absorbent assay support means.
  • Fig. 6 is a side cross-sectional view taken generally along line 6-6 of Fig. 5.
  • the apparatus comprises a guide and positioning means 1 to simultaneously receive and position a plurality of liquid dispensing elements 2, and a cooperating planar absorbent assay support frame 3 to receive and position a planar absorbent assay support 4, such as a sheet of nitrocellulose paper (see Figs. 2 S 3), within the guide and positioning means 1.
  • the guide and positioning means 1 comprises a planar top member 5 having a plurality of bore-type passages 7 formed there through.
  • Each of the passages 7 communicates between the upper and lower surfaces of the top member 5 and is desirably shaped to receive outlet means of the liquid dispensing elements 2, such as, e.g. , pipettes. It is preferred that the liquid dispensing elements 2, such as, e.g. , pipettes. It is preferred that the
  • bore-type passages 7 are generally conical in shape, with the base of the cone being located in the upper surface of the planar member 5. More particularly, it is preferred that the bore-type passages 7 be so dimensioned that the tips of standard pipettes. preferably used as the dispensing elements 2 to transfer assay samples, will protrude through and project beyond the bottom surface of the top planar member 5.
  • the bore-type passages 7 are arranged in the top member 5 in a predetermined pattern, preferably in a rectangular pattern of rows and columns. Since the apparatus of the invention is intended to be used in conjunction with conventional cell culture plates, which have 96 sample wells arranged in 8 rows having 12 wells in each row, the passages 7 are preferably arranged in 8 rows (A-H) , each having 12 of the passages 7 (1-12) spaced relative to one another to correspond to the sample wells in the conventional culture plate.
  • a pair of vertical legs 9 are provided to support the planar top member 5 above a surface. As shown in Fig. 1, the vertical legs 9 comprise two elongated vertical legs 9 extending the full length of the planar member 5. It should be understood, however, that other suitable support means can be used.
  • a pair of grooves 11, or any other equivalent support arrangement, are formed, one in each of the vertical legs 9, to support the absorbent support frame 3 at a predetermined, vertically spaced distance below the bottom of the planar number 5. More particularly, it is desired that the absorbent support frame 3 be supported below the protruding tips of the dispensing elements 2 at a distance such that, when test samples are applied , to the planar absorbent assay support 4, separate discrete "spots" of samples 10 are formed on the absorbent support 4.
  • the distance between the tips of the dispensing elements 2 located in the bores 7 and the upper surface of the absorbent assay support 4 is preferably 2 mm.
  • the absorbent support frame 3 is used to maintain the planar absorbent assay support 4 in a proper alignment relative to the pattern of the bore-type passages 7 when the frame 3 is supported by the grooves 11.
  • the absorbent support frame 3 comprises a slide-like frame having upper and lower cooperating frame members 21 and 22, respectively, designed to clasp and hold an absorbent assay support 4, such as a.sheet of nitrocellulose paper, between them. As shown in Fig.
  • the frame members 21 and 22 are desirably hinged by a hinge connection 23 at one end so that they may be opened to insert the absorbent assay support 4 into the frame member 22 whereupon the frame member 21 is rotated about the hinge connection 23 to close the slide-like frame 3 and thereby clamp the absorbent assay support 4 between them.
  • the frame member 22 is dimensioned such that, when the absorbent assay support 4 is held in the frame 3, an opening 25 in the frame member 21 provides an exposed surface area of the assay support 4 which is greater than the area under the preselected pattern of bore-type passages 7 in the planar member 5, and sufficiently wide such that, discrete the sample "spots" 10 of desired dimensions may be formed.
  • the exposed area of the nitrocellulose assay support 4 is preferably at least 125 mm by 80 mm.
  • the absorbent assay support 4 may include a notch 30 for matching to a- corresponding element 31 on the frame member 22 to insure that the nitrocellulose paper is properly oriented when the frame 3 is inserted into the grooves 11.
  • the invention is preferably designed to allow 5 1 of each of the 96 test samples to be dispensed and simply adsorbed onto the nitrocellulose paper below, and thereby create 96 one cm diameter neighboring rings or sample spots 10 that do not overlap, and which are arranged in a pattern on the assay support 4 corresponding to the pattern of the wells in the cell culture plate from which the samples were derived.
  • the absorbed discrete samples 10 therefor provide a nitrocellulose template.
  • a template holder 100 which may be reusable or disposable, is constructed to hold several, e.g. ten, nitrocellulose assay supports 4 after deposition of the test samples 10.
  • the assay supports 4 are inserted into guide slots 101 such that the inserted supports 4 can be simultaneously submerged into a bath or baths for RIA or ELISA processing, as will be described below.
  • a multiple punch device is provided and illustrated in Fig. 5.
  • the multiple punch device is used in an RIA assay and comprises a base 200 in which a sheet of nitrocellulose paper 4 with the absorbed discrete test samples 10 can be placed.
  • a member 201 is provided with individual means 202 for punching out 96 paper discs, each of which is arranged to circumscribe one of the absorbed test sample spots 10.
  • the member 201 is connected by a hinge 209 to the base 200.
  • Corresponding openings 203 are formed in the base 200 such that the spots punched out by the individual means 202 when the member 201 is rotated about the hinge 209 in a punching operation may be removed from the punch device.
  • An air blow nozzle 204 is mounted at the top of the hinged member 201 to blow air from an air tube 211 through the cutting means 202 via a manifold 210 and thereby fascilitate passage of the 96 punched discs having the test samples 10 thereon through the openings 203 and into 96 tubes 205 arranged in a rack 206 positioned below the base 200.
  • the tubes 205 are arranged in the rack 206 and marked to correspond to the 96 wells in the original cell culture plates and also sequentially left to right.
  • the tubes can go directly into a gamma counter or placed in larger tubes to accommodate the specification of the gamma counter.
  • a pair of handles 207, 208 are provided to facilitate the punching operation.
  • nitrocellulose assay.support 4 is placed in the slide-like frame 3, in the correct orientation, as described above.
  • Test samples of each culture media are pipetted, preferably with a 12-tip multichannel pipette, from each row of twelve wells in a culture plate.
  • the 12 tips 2 of the multichannel pipette are positioned by the conical bore-type passages 7 in the planar member 5 and the test samples in the tips 2 are simultaneously .
  • the template holder 100 is then removed from the bath container containing the blocking solution and dipped into another bath container containing a buffer for a short rinse.
  • the entire holder 100 is then dipped into another bath container contai .ni.ng 125 I antigen (or other isotope) in a 20 mM P-N buffer containing albumin, 1% fish skin gelatin or other blocking solution.
  • concentration and the specific activity of the radio- labelled antigen needed to provide a sufficient number of counts per minute at each test spot in which an antibody is present is determined empirically. This is then shaken at room temperature overnight or shorter (determined empirically) .
  • the template holder 100 is thereafter placed in another bath container containing 20mM phosphate buffer PH 7.5 containing 0.5% NP 40 to remove all nonspecifically bound radio-labelled antigen.
  • the template holder 100 is removed from the bath container of step 8 " nd placed in an oven or air dryer at 37-c to dry the nitrocellulose paper (10 to 15 minutes) .
  • Each sheet of nitrocellulose paper is placed in the base of a multiple punch device 200.
  • the loaded punch is placed on a test tube rack 206 with 96 pre- marked tubes 205 mounted therein, and the top punch means 201 is pressed closed to cut out the 96 discs.
  • An air tubing 211 (lab source) is connected to the nozzle 204 in the top of the punch to blow the 96 paper discs through the openings 203 and into the 96 tubes 205.
  • the premarked tubes 205 are placed into larger tubes to fit into an automatic gamma counter. Each tube is counted for 30 seconds to 1 minute, depending on the level of radioactivity in each tube. (If another isotope is to be used, count with a liquid sintilation counter, depending on the type and energy spectrum of the emitted radiation.)
  • test samples containing the desired antibody are identified by visual inspection of the whole nitrocellulose paper for the development of spots of positive color reaction at the discrete test sample spots deposited on the nitrocellulose paper pursuant to the method of the invention. The locations of the positive test sample spots are correlated to the correct well in the culture plate using the grid on the frame 3. 5
  • the liner may comprise a disposable plastic liner formed to include conical wells to fit the bore- 10 type passages 7 so as to preserve the sterility of the dispensing elements 2 for reuse or to avoid contamination of the planar member 5 and the passages 7 from radioactive or contagious material.
  • a flat horizontal adjustable heater to fit underneath the pipette guide may be used to quickly dry the culture samples absorbed to -nitrocellulose paper, in order to allow multiple applications (on same spots) of cell culture media. Multiple applications are useful at the

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Procédé et dispositif permettant de déposer un ensemble d'échantillons d'analyse liquides sur un support d'analyse absorbant. Selon l'invention, un organe de positionnement et de guidage (1) reçoit et positionne un organe distributeur d'un ensemble d'échantillons d'analyse séparés, de sorte que l'organe distributeur (2) distribue l'ensemble d'échantillons d'analyse selon un motif déterminé. Un support d'analyse absorbant plan (5) est situé dans une position où il est écarté verticalement et aligné sous l'organe de guidage et de positionnement pour recevoir les échantillons d'analyse séparés distribués par l'organe distributeur. Cet agencement permet d'appliquer un ensemble d'échantillons d'analyse ponctuels sur le support d'analyse absorbant, qui les absorbe rapidement, en formant un motif déterminé compatible avec un traitement simultané et efficient dans le système d'analyse immunologique choisi.
PCT/US1989/000193 1988-01-21 1989-01-18 Procede et dispositif d'analyse cellulaire WO1989006788A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019890701731A KR900700867A (ko) 1988-01-21 1989-01-18 세포 스크리닝 방법과 장치

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14657088A 1988-01-21 1988-01-21
US146,570 1988-01-21

Publications (1)

Publication Number Publication Date
WO1989006788A1 true WO1989006788A1 (fr) 1989-07-27

Family

ID=22517997

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/000193 WO1989006788A1 (fr) 1988-01-21 1989-01-18 Procede et dispositif d'analyse cellulaire

Country Status (3)

Country Link
KR (1) KR900700867A (fr)
AU (1) AU3040089A (fr)
WO (1) WO1989006788A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993009435A1 (fr) * 1991-11-09 1993-05-13 Bds Biologicals Limited Detection d'anticorps diriges contre des antigenes nucleaires extractibles et d'autres substances
EP1110613A1 (fr) * 1999-12-23 2001-06-27 Mikron Plastics Technology Support pour embouts de pipette

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4264560A (en) * 1979-12-26 1981-04-28 Samuel Natelson Clinical analytical system
US4341498A (en) * 1980-06-23 1982-07-27 Aluminum Company Of America Method and apparatus for blanking, folding and inserting membrane into container covercap
US4554839A (en) * 1983-10-14 1985-11-26 Cetus Corporation Multiple trough vessel for automated liquid handling apparatus
EP0185330A2 (fr) * 1984-12-18 1986-06-25 Cetus Corporation Système de traitement pour des échantillons multiples
US4713165A (en) * 1986-07-02 1987-12-15 Ilex Corporation Sensor having ion-selective electrodes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4264560A (en) * 1979-12-26 1981-04-28 Samuel Natelson Clinical analytical system
US4341498A (en) * 1980-06-23 1982-07-27 Aluminum Company Of America Method and apparatus for blanking, folding and inserting membrane into container covercap
US4554839A (en) * 1983-10-14 1985-11-26 Cetus Corporation Multiple trough vessel for automated liquid handling apparatus
EP0185330A2 (fr) * 1984-12-18 1986-06-25 Cetus Corporation Système de traitement pour des échantillons multiples
US4713165A (en) * 1986-07-02 1987-12-15 Ilex Corporation Sensor having ion-selective electrodes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCHLEICHER et al., Minifold Bulletin, No. 358 3/583, 1981. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993009435A1 (fr) * 1991-11-09 1993-05-13 Bds Biologicals Limited Detection d'anticorps diriges contre des antigenes nucleaires extractibles et d'autres substances
EP1110613A1 (fr) * 1999-12-23 2001-06-27 Mikron Plastics Technology Support pour embouts de pipette

Also Published As

Publication number Publication date
AU3040089A (en) 1989-08-11
KR900700867A (ko) 1990-08-17

Similar Documents

Publication Publication Date Title
US5011779A (en) Apparatus for rapid deposition of test samples on an absorbent support
US4682890A (en) Microsample holder and carrier therefor
EP0154686B1 (fr) Plaque de support et dispositif d'examen à récipients multiples pour les essais immunologiques
US4427415A (en) Manifold vacuum biochemical test method and device
AU613621B2 (en) Sample ring for clinical analyzer network
US5324481A (en) Carousel for assay specimen carrier
DE69214013T2 (de) Reagenzienpackung für Immunoassays
US4787988A (en) Cell harvester
US5246665A (en) Heat and air flow control for assay carrier
US4952518A (en) Automated assay machine and assay tray
US5779907A (en) Magnetic microplate separator
US8298498B2 (en) Apparatus and method for handling fluids for analysis
JPH0795012B2 (ja) スライドガラス上に複数の液体試料を同時に引入れるための方法とその装置
US5320809A (en) Retrofit kit for changing single immunoassay instrument to flexible multiple immunoassay instrument
US8877141B2 (en) System for preparing arrays of biomolecules
JPH05188059A (ja) 試薬ビンとキャップ
JP2007163465A (ja) 免疫学的検定のための製品およびプロセス
JPS58501521A (ja) 自動免疫定量システム
JPH02502569A (ja) 抗体マトリツクス装置
US4446104A (en) Apparatus for transferring sample vessels into collecting vessels
WO2002102962A2 (fr) Trous d'acces de plaque de filtre multipuits pour appareil d'essai multipuits
US6663836B1 (en) Apparatus and method for alphanumerically identifying and arranging test tubes
US4713349A (en) Templet for simultaneous screening of several antibodies and method of using the same
EP0574243A2 (fr) Dispositif de transfert pour analyses
JP4026686B2 (ja) 薄組織断面の免疫標識化装置および方法

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AU BG DK FI HU JP KR NO RO SU

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE FR GB IT LU NL SE