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A method for diagnosis using one or more cells, and apparatus intended therefor

Info

Publication number
WO1989003038A1
WO1989003038A1 PCT/EP1988/000879 EP8800879W WO1989003038A1 WO 1989003038 A1 WO1989003038 A1 WO 1989003038A1 EP 8800879 W EP8800879 W EP 8800879W WO 1989003038 A1 WO1989003038 A1 WO 1989003038A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
microwave
temperature
sample
method
oven
Prior art date
Application number
PCT/EP1988/000879
Other languages
French (fr)
Inventor
Mathilde Elisabeth Boon
Lanbrecht Piet Kok
Original Assignee
Mathilde Elisabeth Boon
Lanbrecht Piet Kok
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat

Abstract

The method according to the present invention accelerates known diagnosis methods, as ELISA and (immuno-)staining, by heating samples to be used and controlling the temperature thereof. Preferably this is achieved by microwave radiation.

Description

"A method for diagnosis using one or more cells, and apparatus intended therefore"

More and more, methods for diagnosis, such as iiruminostaining, ELISA, etc. are used in hospitals for obtaining diagnosis of patients.

The present invention has for its object to accelerate such diagnosis methods, such as to be able to obtain results on shorter notice, for instance to be able to have such results available for out-patients.

The present invention is defined by the annexed claim 1. The heating can be obtained by means of hot air, but preferably the heating is achieved by means of microwave radiation inducing heating internally in the sample, which further accelerate the heating and therefore the diagnosis. Further stirring of the samples during treatment can be dispensed with at using microwave radiation.

A thermometer (e.g. using a fibre optic) is expensive and can be dispensed with in a further preferred method (claim 4) and/or a microwave oven according to the sub-claims. Further details, features and advantages of the present invention will be clarified reading the following description, referring to the annexed drawing, in which show:

Fig. 1 a perspective view of a microwave oven in which a blood or sputum sample is placed, Fig. 2 a table provided with cooling means, on which a sample is to be placed,

Fig. 3, 4 and 5 diagrams in which in a preferred embodiment of the method according to the present invention, the temperature was measured as function of time. In a microwave oven 2, provided with a microwave antenna 1, a plate 4 is placed on a table 3. The plate 4 was pre-cooled, e.g. deepfreezed on -20° C. The sample 6, e.g. a drop of sputum, is placed on a synthetic plate 5, such as that microwaves will reach the drop 6. In fig. 2 table 3* is provided with a conduit 7 in which a cooling fluid can be led. Immunostaining can take place in sample 9, received between two plates 8.

By means of the pre-cooled plate 4 (fig. 1, 3).the temperature of a drop of 2,5 ml only rises from about 20° C to about 35° C during a period of four minutes and at supplying a power of 150 Watt to the antenna 1. This rise of temperature is physiologically not harmful to a blood or sputum drop. At lowering the supply power to 80 Watt the temperature in a drop of 2,5 ml (fig. 4) will be more constant, as it also will be more constant at supplying a power of 150 Watt to a drop of 30 ul (fig. 5).

The diagrams of fig. 3, 4 and 5 are measured with thermometers using an optic fibre. The tests were performed in a domestic microwave oven (Miele R M696).

The control of temperature can also be achieved by other means, such as a buffer of electrolyte (water) in a microwave oven or a wet sheet of paper. The buffer of electrolyte will receive radiation supplied by the microwave antenna.

The above described embodiment uses a domestic microwave oven. Between the date of priority of this application and filing date thereof, a more sophisticated microwave oven became available, the Bio-Rad H2500 Microwave Processor, in which the temperature can be controlled more easily, by setting the power to be supplied to the antenna and by means of a temperature sensing element.

More details about immunostaining and ELISA etc. including experiments performed, were published by the present inventors in the second edition of their book: Microwave Cookbook of Pathology, ISBN 90-71421-10-4, Leiden, 1988, especially chapters 14, 15, 16, 17, 20, 24, 25, 26 of which copies are annexed.

Claims

1. A method for diagnosis using one or more cells in one or more samples, e.g. from blood, sputum or urine, such as (immuno-)staining, ELISA, etc, comprising incubation and washing steps, in which during one or more incubation steps the sample is heated to a temperature above room temperature, and in which this temperature is controlled such as to prevent damage to the sample, due to overheating.
2. Method of claim 1, in which the heating is performed by a microwave radiation.
3. Method of claim 1 or 2, in which the temperature is controlled at the value of substantially 80° C, preferably 50° C or 40° C.
4. Method of claim 1, 2 or 3, in which the sample is placed on a pre-cooled plate in a microwave oven.
5. An apparatus for carrying out the method of one of the claims 1-4, in which a sample has a heat capacity which is large relative to the capacity of the sample and relative to the heating time.
6. A microwave oven for carrying out the method of one of the claims 1-4, in which a sample holder is provided with cooling means.
PCT/EP1988/000879 1987-09-30 1988-09-30 A method for diagnosis using one or more cells, and apparatus intended therefor WO1989003038A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
NL8702338A NL8702338A (en) 1987-09-30 1987-09-30 Method for the running of (bio) chemical or (micro) biological reactions, using micro-waves, therefrom using methods and apparatus therefor.
NL8702338 1987-09-30

Publications (1)

Publication Number Publication Date
WO1989003038A1 true true WO1989003038A1 (en) 1989-04-06

Family

ID=19850685

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1988/000879 WO1989003038A1 (en) 1987-09-30 1988-09-30 A method for diagnosis using one or more cells, and apparatus intended therefor

Country Status (2)

Country Link
NL (1) NL8702338A (en)
WO (1) WO1989003038A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9300976A (en) * 1993-06-07 1995-01-02 Inst Voor Agrotech Onderzoek A method for altering the kinetics of a reaction in which at least one macromolecule is involved which comprises treating the macromolecule with electromagnetic energy, the application of this method for the optimization of processes, in which the macromolecule is involved, and a macromolecule with altered reactivity.
WO2002014868A1 (en) * 2000-08-16 2002-02-21 Council Of Scientific And Industrial Research A rapid method for microwave mediated enzyme-linked immunosorbent assay
WO2004088315A1 (en) * 2003-03-31 2004-10-14 Council Of Scientific And Industrial Research Rapid heat-mediated method for enzyme-linked immunosorbent assay procedure
WO2008009868A2 (en) * 2006-07-21 2008-01-24 Exonhit Therapeutics Sa Methods and tools for the therapy of neurodegenerative pathologies
WO2010028164A1 (en) * 2008-09-05 2010-03-11 The Government Of Usa, As Repesented By The Secretary, Department Of Health And Human Services Device and method for microwave assisted cryo-sample processing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3796639A (en) * 1971-09-07 1974-03-12 Gen Electric Biological incubator
US4327180A (en) * 1979-09-14 1982-04-27 Board Of Governors, Wayne State Univ. Method and apparatus for electromagnetic radiation of biological material
EP0058428A2 (en) * 1981-02-18 1982-08-25 Eisai Co., Ltd. An enzyme immuno-assay for simultaneously measuring a plurality of samples and test vessel for carrying out this method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3796639A (en) * 1971-09-07 1974-03-12 Gen Electric Biological incubator
US4327180A (en) * 1979-09-14 1982-04-27 Board Of Governors, Wayne State Univ. Method and apparatus for electromagnetic radiation of biological material
EP0058428A2 (en) * 1981-02-18 1982-08-25 Eisai Co., Ltd. An enzyme immuno-assay for simultaneously measuring a plurality of samples and test vessel for carrying out this method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
C.R. Acad. Sc. Paris, volume 281, serie D, 22 September 1975, A.-J. Berteaud et al.: "Radio-biologie. - Auction D'un rayonnement électromagnétique à longueur d'onde millimétrique sur la croissance bactérienne", pages 843-845 *
Chemical Abstracts, volume 106, 13 April 1987, (Columbus, Ohio, US), A.E. Ashford et al.: "Temperature control in Lowicryl K4M and glycol methacrylate during polymerization: is there a low-temperature embedding method? see page 294 *
Laboratory Investigation, volume 57, no. 5, May 1987, The United States and Canadian Academy of Pathology, Inc., (US), G.R. Login et al.: "Methods in laboratory investigation. Rapid microwave fixation of human tissues for light microscopic immunoperoxidase identification of diagnostically useful antigens", pages 585-591 *
Medical Laboratory Sciences, volume 44, 1987, K.Y. Chiu: "Use of microwaves for rapid immunoperoxidase staining of paraffin sections", pages 3-5 & Chemical Abstracts, volume 106, 22 June 1987, page 337 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9300976A (en) * 1993-06-07 1995-01-02 Inst Voor Agrotech Onderzoek A method for altering the kinetics of a reaction in which at least one macromolecule is involved which comprises treating the macromolecule with electromagnetic energy, the application of this method for the optimization of processes, in which the macromolecule is involved, and a macromolecule with altered reactivity.
WO2002014868A1 (en) * 2000-08-16 2002-02-21 Council Of Scientific And Industrial Research A rapid method for microwave mediated enzyme-linked immunosorbent assay
US6498016B1 (en) 2000-08-16 2002-12-24 Council Of Scientific And Industrial Research Rapid method for enzyme-linked immunosorbent assay
WO2004088315A1 (en) * 2003-03-31 2004-10-14 Council Of Scientific And Industrial Research Rapid heat-mediated method for enzyme-linked immunosorbent assay procedure
WO2008009868A2 (en) * 2006-07-21 2008-01-24 Exonhit Therapeutics Sa Methods and tools for the therapy of neurodegenerative pathologies
WO2008009868A3 (en) * 2006-07-21 2008-05-08 Exonhit Therapeutics Sa Methods and tools for the therapy of neurodegenerative pathologies
WO2010028164A1 (en) * 2008-09-05 2010-03-11 The Government Of Usa, As Repesented By The Secretary, Department Of Health And Human Services Device and method for microwave assisted cryo-sample processing

Also Published As

Publication number Publication date Type
NL8702338A (en) 1989-04-17 application

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