WO1988009809A3 - Systeme cellulaire pour l'expression d'une proteine recombinante sans production de virus - Google Patents

Systeme cellulaire pour l'expression d'une proteine recombinante sans production de virus Download PDF

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Publication number
WO1988009809A3
WO1988009809A3 PCT/US1988/001957 US8801957W WO8809809A3 WO 1988009809 A3 WO1988009809 A3 WO 1988009809A3 US 8801957 W US8801957 W US 8801957W WO 8809809 A3 WO8809809 A3 WO 8809809A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
recombinant protein
virus
production
cell system
Prior art date
Application number
PCT/US1988/001957
Other languages
English (en)
Other versions
WO1988009809A2 (fr
Inventor
John F Weaver
M Michele Manos
Yakov Gluzman
Original Assignee
Cetus Corp
Cold Spring Harbor Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp, Cold Spring Harbor Lab filed Critical Cetus Corp
Publication of WO1988009809A2 publication Critical patent/WO1988009809A2/fr
Publication of WO1988009809A3 publication Critical patent/WO1988009809A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des cellules de mammifères qui sont transformées de manière stable avec un système d'expression compatible pour exprimer une protéine recombinante dans laquelle un facteur nécessaire à l'expression de la protéine recombinante est absent de la cellule transformée de manière stable, cellules mammifères dans lesquelles est introduit un vecteur défectueux au niveau de la réplication et qui exprime le facteur requis. L'invention concerne également des procédés d'obtention de l'expression de protéines recombinantes à partir d'une telle cellule.
PCT/US1988/001957 1987-06-12 1988-06-09 Systeme cellulaire pour l'expression d'une proteine recombinante sans production de virus WO1988009809A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6168487A 1987-06-12 1987-06-12
US061,684 1987-06-12

Publications (2)

Publication Number Publication Date
WO1988009809A2 WO1988009809A2 (fr) 1988-12-15
WO1988009809A3 true WO1988009809A3 (fr) 1989-01-12

Family

ID=22037435

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/001957 WO1988009809A2 (fr) 1987-06-12 1988-06-09 Systeme cellulaire pour l'expression d'une proteine recombinante sans production de virus

Country Status (2)

Country Link
IL (1) IL86699A0 (fr)
WO (1) WO1988009809A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843742A (en) * 1994-12-16 1998-12-01 Avigen Incorporated Adeno-associated derived vector systems for gene delivery and integration into target cells
US6221349B1 (en) 1998-10-20 2001-04-24 Avigen, Inc. Adeno-associated vectors for expression of factor VIII by target cells
US6200560B1 (en) 1998-10-20 2001-03-13 Avigen, Inc. Adeno-associated virus vectors for expression of factor VIII by target cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Bio/Technology, volume 6, no. 3, March 1988, (New York, US), J.F. Weaver et al.: "Production of recombinant human CSF-1 in an inducible mammalian expression system", pages 287-290 *
Molecular and Cellular Biology, volume 4, no. 8, August 1984, American Society for Microbiology, K. Van Doren et al.: "Efficient transformation of human fibroblast by adeno-virus-Simian Virus 40 recombinants", pages 1653-1656 *
Proceedings of the National Academy of Sciences of the USA, volume 82, no. 11, June 1985, (Washington, D.C., US), M. Yamada et al.: "Overproduction of the protein product of a non-selected foreign gene carried by an adenovirus vector", pages 3567-3571 *
Science, volume 230, 18 October 1985, E.S. Kawasaki et al.: "Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1)", pages 291-296 *

Also Published As

Publication number Publication date
IL86699A0 (en) 1988-11-30
WO1988009809A2 (fr) 1988-12-15

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