WO1987006114A1 - Apparatus for cell sampling - Google Patents
Apparatus for cell sampling Download PDFInfo
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- WO1987006114A1 WO1987006114A1 PCT/JP1987/000238 JP8700238W WO8706114A1 WO 1987006114 A1 WO1987006114 A1 WO 1987006114A1 JP 8700238 W JP8700238 W JP 8700238W WO 8706114 A1 WO8706114 A1 WO 8706114A1
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- Prior art keywords
- cells
- edge
- cell collection
- cervix
- cell
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
Definitions
- the present invention relates to a cell collection instrument used for collecting cells as a specimen, for example, when performing cytodiagnosis for uterine cancer. . Background art
- Such cervical cancer screening is performed by collecting cells such as the cervix and cervix using a cell collection device, but early stage cancer such as cervical cancer is performed. It is especially important to make a cytological diagnosis in the cervix for the discovery of the disease.
- cancers as cervical cancer have a depth of 100 to 300.
- basal cells and parabasal cells located in B and gradually appear in middle cells and surface cells located at depths of 30 to 100 ⁇ , ⁇ . Reliable collection of these cells, especially basal cells and parabasal cells, will lead directly to early detection of cancer.
- instruments used for collecting such seed cells include a cotton swab as shown in FIG. 7 and a snorter shown in FIG. Or brushes as shown in Fig. 9, but each had the drawbacks listed below.
- Abrasions are likely to occur at the sampling site and blood is often bleeding.
- the tip is sharp, it is very dangerous to accidentally pierce the uterus or vagina, etc., which is extremely dangerous.
- the main purpose of the present invention is to narrow the insertion site, such as the cervix, or to detect uterine aging or menopause after menopause. Even if the insertion site is hardened and becomes difficult to spread due to the onset of the disease, painful re-scaling may occur at the site.
- An object of the present invention is to provide a cell collection device that can be safely and securely inserted without difficulty.
- Another object of the present invention is to provide a cell collection device that can reliably collect cells at a depth effective for the detection of early stage cancer by a simple operation. .
- Another object of the present invention is to obtain a cell collection device that has good adherence of the collected cells and has excellent smearability on slide glass. .
- the cell-collecting device of the present invention uses a small rod-shaped handle at the tip of a handle to remove cells.
- the scraping portion as a whole is formed in a tapered cone shape, and a hand is provided on a side surface of the cone-shaped scraping portion. It is characterized in that a saw-tooth-shaped projection rising toward the base end side of the drill is formed in a spiral shape, and the edge is formed on one side of the projection. It is something.
- the scraping portion is formed in a helical shape, and a spiral edge is formed on the side surface of the rubbing portion. Even if the insertion site is narrow, such as the cervix, or if the site is hardened due to cancer, etc. By simply pressing the tip of the insertion part into the entrance of the insertion site and rotating it, it can be inserted smoothly in a screw-in manner. There is little worry about occasional pain or damage to the insertion site.
- the edge can easily remove cells around the entire inner wall of the insertion site.
- FIG. 1 is a plan view of the whole showing one embodiment of the present invention
- FIG. 2 is an enlarged view of a conical scraping portion
- FIG. 3 is a cross-sectional view taken along line AA in FIG. 4 and 5 are enlarged views of the spatula and its side view
- FIG. 6 is a side view of the main part showing the method of smearing the collected cells
- FIGS. 9 The figure shows The best mode for carrying out the invention, which is a plan view of a conventional cell collection device.
- the sampling device 1 shown in its entirety in FIG. 1 has a configuration in which a cone-shaped scraping portion 3 and a spatular scraping portion 4 are provided at both ends of a nozzle 2 having a small-diameter rod shape. These are formed integrally from a relatively flexible and elastically deformable synthetic resin.
- the handle 2 is easy to hold because the cross section is circular or polygonal and the surface is roughened by fine irregularities.
- the structure is easy to operate, but still retains a degree of flexibility that does not cause unnecessary deformation during operation.
- the above-mentioned conical scraping part 3 is for separating cells in the cervix and collecting them, and is a thin part for facilitating elastic deformation at the tip of the above-mentioned handle 2. It is formed via the diameter portion 2a, and has a structure as specifically shown in FIGS. 2 and 3. That is, the conical abrasion portion 3 is formed to be thick enough to be inserted into the cervix without pain, and it is possible to cope with individual differences in the insertion site.
- the conical or pyramid-shaped taper is formed in a conical shape, such as a cone or a pyramid, and on the side surface of the conical scraping portion 3, a spiral is formed around the scraping portion. Edge 5 is formed.
- This edge 5 ' A saw-tooth-shaped projection S rising up toward the base end side of the handle 2 on the side surface of the scraping portion 3 in a spiral shape, and formed on the ridge portion of the projection S. It is what you do.
- a plurality of grooves 7 extending in the X-axis direction of the rubbing part are formed at regular intervals around the rubbing part 3 on the side surface of the conical rubbing part 3,
- An edge 8 is formed at the groove edge of the groove ⁇ so as to face in the circumferential direction of the rubbing part.
- the tip 3a of the conical abrasion 3 is formed in a spherical shape so that it can be safely inserted into the cervix.
- the degree of softness of the small-diameter portion 2a does not unnecessarily deform when the conical abrasion portion 3 is inserted into the cervix.
- FIG. 6 when the rubbing part 3 is pressed onto the slide glass when the rubbing is applied to the slide glass and the slide glass, as shown in FIG. Gulf is preferable to be able to bend
- the spatula 4 formed at the proximal end of the node 2 is for collecting cells in the posterior fornix or uterine vagina, and has a certain width.
- a flexible plate-shaped member having an arc-shaped tip at the top and bottom of the plate-shaped member 4a.
- a large number of saw-toothed ribs 10 long in the width direction are formed at regular intervals, and the ribs 10 are formed by these ribs 10.
- the edge 11 is formed so as to face the tip side of the dollar 2.
- the plate-shaped body 4a is located at a position between the edge and the edge forming part 4b.
- a plurality of cuts 12 are provided to increase the height of the edge, and the edge forming portion 4b of the plate-like body 4a is formed to be thinner than the edge non-forming portion 4c. It has been.
- the height of the edges 5, 8, and 11 in the conical scraping part 3 and the spatula-like scraping part 4 may damage the stroma even from the superficial cells to the basal cells. It is preferable to set the range from 30 to 500a, so that the sample can be collected without any trouble. The range is 50 to 350m in the middle. Is most preferred.
- a synthetic resin material forming the above-mentioned collection instrument 1 a synthetic resin which has good cell adhesion and is superior in transferability to slide glass is preferable, for example.
- polyolefins such as polystyrene, polyvinylene, * ⁇ acid vinyl copolymer, polyethylenevinyl alcohol copolymer, etc.
- Polystyrene, Polystyrene, Polystyrene such as ABS, Polyimide, Polyvinyl Alcohol copolymer resin, etc. is there .
- these surfaces are formed as a rough surface having minute concaves and convexes as a whole. You can do that too.
- the conical abrasion part 3 When the cells in the cervix cervix are collected by the conical abrasion part 3 in the above-described cell collection instrument 1, the conical abrasion part 3 is pressed against the resection of the cervix and the collection instrument is pressed. Screw it into the cervix by rotating 1. Therefore, in cases where the cervix was narrow, cancer or other parts of the cervix were hardened and became difficult to spread. Even so, the conical abrasion portion 3 is gradually pushed down to the portion, and can be smoothly inserted with a widespread force S. There is no risk of damaging the site.
- the spiral edge 5 removes the cells on the inner wall of the cervix cervix. Are collected and collected. At this time, if the handle 2 is slightly rotated, and the cells are removed by the edge 8 at the groove edge, the entire circumference of the inner wall of the cervix and cervix can be spread. The cells can be reliably collected.
- the tip of the sampler 1 is deformed as shown in FIG. Press the side surface of the conical rubbing part 3 against the slide glass 15 with a slight force, and turn the conical rubbing part 3 forward while turning the handle 2. Let it slide. As a result, the cells adhered to the edges 5 and 8 are detached, and are reliably smeared on the slide glass 5.
- the spatula 4 When the cells of the posterior fornix of the vagina or the vagina are to be collected by the spatula 4, use the spatula 4.
- the edge-forming portion 4b is pressed against the collection site, and the cells are removed by the edge 11 thereof.
- the cells collected at the spatula scraping part 4 are pressed against the slide glass 15 to slide the cells forward. S can be applied to slide glass.
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Abstract
An apparatus used for sampling cells from the cervix of the womb in the case of medical examination of a cell applied to the diagnosis of cancer of the womb. Technical subject of this invention is to constitute said apparatus for cell sampling so that even when a part into which said apparatus is inserted has become so narrow and hard as the cervix of a woman after the menopause or has swollen or has become too hard to be easily expanded owing the cancer or other diseases, said apparatus can be inserted into said part safely and surely. According to this invention, the apparatus for cell sampling has a scratching part (3) for scraping cells on the forward end of a handle (2) having a thin cylindrical shape, said scratching part (3) having as a whole a gimlet-like shape tapering toward the end, on the side thereof saw-toothed projections (6) projected in the direction of the base end of said handle (2) being formed in a spiral form, on the ridges of said projections (6) edges (5) being formed; so that said apparatus can be inserted, as if screwed, into said cervix of the womb by pushing the forward end of said scratching part (3) against the entrance of the cervix and rotating it.
Description
明 細 Details
発明 の 名称 Title of invention
細胞採取器具 技術分野 Cell collection instrument
本発明 は 、 例 え ば子宮癌 を 対象 と す る 細胞診 を 行 う 場合等 に 、 検体 と し て の細胞 を 採取 す る の に使用 さ れ る 細胞採取器具 に 関す る も の で あ る 。 背景技術 The present invention relates to a cell collection instrument used for collecting cells as a specimen, for example, when performing cytodiagnosis for uterine cancer. . Background art
近年 、 子宮癌 の発生率 は次第 に増加 し て お り 、 そ の 予 防 の た め に 定 期 検 診 に よ る 早 期 発 見'が望 ま れ て い る 。 In recent years, the incidence of uterine cancer has been gradually increasing, and it is hoped that early detection by regular screening will be conducted to prevent it.
かか る 子宮癌特 に子宮頸癌 の定期検診 は 、 細胞採取 器 具 に よ リ 子宮膣部及 び子宮頸管部 な ど の細胞 を 採取 し て行 う が、 子宮頸癌 な ど の初期癌 の発見 に は 、 子宮 頸管内 の細胞診断 を 行 う こ と が特 に 重要 で あ る 。 し か も 、 こ の よ う な子宮頸癌 な ど の癌 は 、 深度 1 0 0〜 3 0 0 Such cervical cancer screening, especially cervical cancer, is performed by collecting cells such as the cervix and cervix using a cell collection device, but early stage cancer such as cervical cancer is performed. It is especially important to make a cytological diagnosis in the cervix for the discovery of the disease. However, such cancers as cervical cancer have a depth of 100 to 300.
B に 位置す る 基底細胞や旁基底細胞 な ど で発生 し 、 そ れが次第 に深度 3 0〜 1 0 0 μ, α に位置す る 中層細胞や 表層細胞 に現われ る た め 、 こ れ ら の細胞特 に基底細胞 や旁基底細胞 を 確実 に 採取 す る こ と が癌 の早期発見 に 直結 す る こ と に な る 。 These occur in basal cells and parabasal cells located in B, and gradually appear in middle cells and surface cells located at depths of 30 to 100 μ, α. Reliable collection of these cells, especially basal cells and parabasal cells, will lead directly to early detection of cancer.
と こ ろ で 、 上記子宮頸管 ほ 、 も と も と 非常 に 狭 い部 分'で あ つ て 、 個人差 に よ っ て も 弾性や大 き さ 等が著 し
< 異 な つ て い る 力 特 に 、 子宮頸癌発生の リ ス ク 力 S t¾ い と さ れ て い る 閉経後の 婦人 は、 子宮頸管が萎縮 し て 固 く な つ て い る 例が多 く 、 し か も 、 癌が発症 し て い る ¾ *fcr に は さ ら に 固ィ匕 し た リ 肥大 し て い る 場合が 多い た め 、 採取器具 を 痛 みや傷 を 生 じ る こ と な く 安全 に挿入 し 、 且っ該頸管の内壁全周 に わた つ て細胞 を 確実 に 採 取す る の は非常 に困難 な が極 め て 多 い 。 At the same time, the above cervix was originally a very narrow part, and its elasticity and size were remarkable depending on individual differences. <Different forces, especially in postmenopausal women who are considered to be at risk for developing cervical cancer, St. In many cases, cancer has developed. ¾ * fcr is often hardened and re-enlarged. It is extremely difficult, but extremely difficult, to insert the cells safely and to reliably collect the cells over the entire inner wall of the cervical canal.
而 し て、 従来 、 こ の 種細胞の採取 に使用 さ れ て い る 器具 と し て 、 第 7 図 に示す よ う な綿棒や第 8 図 に示す ょ ラ な ス ノ 一テ ル 、 あ る い は第 9 図 に示す よ う な ブ ラ シ な どが知 ら れて い た が れ ら は そ れぞれ次 に 列記 'す る よ う な 欠点 を 有 し て い た Conventionally, instruments used for collecting such seed cells include a cotton swab as shown in FIG. 7 and a snorter shown in FIG. Or brushes as shown in Fig. 9, but each had the drawbacks listed below.
A 綿 A cotton
a 綿の柔軟性が大 き く 且つ分泌物 に よ り 湿潤 し 易 い た め 、 擦過力が小 さ く 、 深度 の大 き い部位 の細 胞 を 十分 に採取す る こ と が で き な い 。 ま た 、 力 を 加 え す ぎ る と 棒が折れ、 非常 に 危険 で あ る 。 a Because cotton is flexible and easily wetted by secretions, it is not possible to sufficiently collect cells from sites with a small abrasion and a large depth. No. Also, if you apply too much force, the rod will break, which is very dangerous.
b 分泌物血球 な ど検査 に不必要 な も の を 細胞 と一 緒 に 大里 に 採取 し 易 い た め 、 細胞 を 顕微鏡検査す る 際 に 夾雑物が 多 く 、 ΙΠ 的 の細胞 を 発見す る の に ΙΠΙがかか る 。 b It is easy to collect unnecessary things such as secretory blood cells for the test together with the cells in Osato.Therefore, when the cells are examined by microscopy, many foreign substances are found and specific cells are found. It takes a while.
c 採取 し た細胞が綿 に絡 み付 き 易 く 、 且つ離れ に ぐ い の で 、 そ の細胞 を ス ラ イ ド、 ガ ラ ス 上 に 塗抹す る 作業が非常 に 困難 で あ る 。
B ス ノヽ β — テ ル c Since the collected cells are easily entangled with cotton and are easily separated, it is very difficult to smear the cells on slides and glass. B Snow ヽβ — Ter
a 細胞 を 大量 に 採取 す る こ と が で き る が、 間質細 胞 な ど の不要細胞 を 含 め て組織塊状 に 剝離 す る こ と に な る た め 、 採取 し た細胞 の 重積性が強 く 、 顕 微鏡検査 の際 に個 々 の細胞が何層 に も 重 な っ て 見 る た め構造が判別 し に く く 、 判定 を 困難 に し 易 い o a It is possible to collect a large amount of cells, but it is necessary to separate unnecessary cells such as interstitial cells into tissue clumps. The structure is difficult to discriminate because the individual cells are superimposed on multiple layers during microscopy, making it difficult to judge.o
b 採取 部位 に擦過傷 を 生 じ 易 く 、 出 血す る こ が 多 い 。 b Abrasions are likely to occur at the sampling site and blood is often bleeding.
c 最 も 重要 な 子宮頸管内 の細胞 を 採取 す る こ と が で き な レ, c It is not possible to collect the most important cells in the cervix ,
9- C'、 ブ ラ シ 9-C ', brush
a 先端が と が っ て い る た め 、 子宮 、 膣内等 を 誤 つ -て刺突す る お そ れが 多 く 、 非常 に 危険 で あ る 。 a Because the tip is sharp, it is very dangerous to accidentally pierce the uterus or vagina, etc., which is extremely dangerous.
b 子宮頸管カゝ ら ブ ラ シ を 抜 く と き 、 採取 し た細胞 が植毛繊維か ら 脱落 し 易 い。 b When the brush is removed from the cervical canal, the collected cells can easily fall off from the flocked fibers.
c 採取' し た細胞 を ス ラ イ ド ガ ラ ス に 塗抹す る 際 、 植毛繊維部 を 強 く 押 し つ け て し ぼ り 取 る よ ラ に し な けれ ば な ら ず、 熟練 を 要 し 、 ま た 、 細胞がか な り ブ ラ シ 側 に 残留 す る こ と を 避 け る こ と が で き な い o 発明 の 開示 c When applying the collected cells to a slide glass, it is necessary to skillfully press the flocked fiber part firmly and squeeze it out. In addition, it is not possible to prevent cells from remaining on the brush side considerably.o Disclosure of the invention
本発明 の主た る 目 的 は 、 子宮頸管の よ う に挿入部位 が狭小 で あ っ た り 、 閉経後 の子宮 の 老化 あ る い は癌 等
の発症 に よ リ 挿入部位が硬化 し て 広が り に く く な っ て い る よ う な場合 で あ っ て も 、 該部位 に 、 痛 み を 伴 っ た リ 傷 を 生 じ た り す る こ と な く 安全且つ確実 に挿入 す る こ と の で き る 細胞採取器具 を 提供す る こ と に あ る 。 The main purpose of the present invention is to narrow the insertion site, such as the cervix, or to detect uterine aging or menopause after menopause. Even if the insertion site is hardened and becomes difficult to spread due to the onset of the disease, painful re-scaling may occur at the site. An object of the present invention is to provide a cell collection device that can be safely and securely inserted without difficulty.
本発明 の 他の 目 的 は 、 初期癌 の発見 に 有効 な深 さ の 細胞 を 簡単 な操作 に よ り 確実 に 採取す る こ と が で き る 細胞採取器具 を 提供す る こ と に あ る 。 Another object of the present invention is to provide a cell collection device that can reliably collect cells at a depth effective for the detection of early stage cancer by a simple operation. .
本 発 明 の 別 の 目 的 は 、 採 取 し た 細 胞 の 付 着性 が よ く 、 且つ ス ラ イ ド ガ ラ ス へ の塗抹性 に 優れた細胞採取 器具 を 得 る こ と に あ る 。 Another object of the present invention is to obtain a cell collection device that has good adherence of the collected cells and has excellent smearability on slide glass. .
上 記 ほ 的 を 達 成 す る た め 、 木 発 明 の 細 胞採取 器 具 は 、 細径棒状 を すハ ン ド ル.の先端 に 、 '細胞 を 搔 き 取 る た め あ エ ツ ジ を 備 え た擦過部 を 形成 し て な る 細胞採 取器具 に お い て 、 上記擦過部 を 全体 と し て先細 り を な す錐状 に 形成 し 、 該錐状擦過部の側面 に ハ ン ド ル の基 端側 を 向 い て立上が っ た鋸歯状 の突起 を 螺旋状 に 形成 す る と 共 に、 該突起の铙部 に 上記 エ ッ ジ を 形成 し た こ と を特徴 と す る も の で あ る 。 In order to achieve the above objective, the cell-collecting device of the present invention uses a small rod-shaped handle at the tip of a handle to remove cells. In a cell-collecting instrument having a scraping portion provided with the above, the scraping portion as a whole is formed in a tapered cone shape, and a hand is provided on a side surface of the cone-shaped scraping portion. It is characterized in that a saw-tooth-shaped projection rising toward the base end side of the drill is formed in a spiral shape, and the edge is formed on one side of the projection. It is something.
上記構成 を 有 す る 本発明 の細胞採取器具 に よ れ ば、 擦 過 部 を 雜 状 と し 、 且 つ 該 擦 過 部 の 側 面 に 螺旋状 の エ ッ ジ を 形成 し て あ る の で 、 子宮頸管の よ う に 挿入部 位が狭小 で あ っ た リ 、 癌 な ど に よ り そ の部分が硬化 し て 広力 S り に く く な つ て い る 場合等 で も 、 擦過部 の先端 を 挿入部位 の入 り 口 に押 し 付 け て 回転 さ せ る だ け で 、 そ れ を ね じ 込 み式 に 円滑 に 挿入す る こ と が で き 、 挿入
時 に 痛 み を 伴 っ た り 、 挿入 部位 を 傷付 け る 心配が ほ と ん ど な い 。 According to the cell collecting instrument of the present invention having the above-described configuration, the scraping portion is formed in a helical shape, and a spiral edge is formed on the side surface of the rubbing portion. Even if the insertion site is narrow, such as the cervix, or if the site is hardened due to cancer, etc. By simply pressing the tip of the insertion part into the entrance of the insertion site and rotating it, it can be inserted smoothly in a screw-in manner. There is little worry about occasional pain or damage to the insertion site.
ま た 、 擦過部 を 挿入 し た あ と は 、 そ れ を 単 に 铀線方 向 に 引 き 抜 く だ け で 、 上記 エ ッ ジ に よ り 挿入部位 の 内 壁全周 の細胞 を 簡 単且つ確実 に 搔 き 取 る こ と が で き 、 且つ 、 搔 き 取 っ た細胞 を エ ッ ジ に付着 さ せ て取 り 出 す こ と 力 S で き る 。 In addition, once the abraded part is inserted, simply pull it out in the X-ray direction, and the edge can easily remove cells around the entire inner wall of the insertion site. In addition, it is possible to reliably remove the cells, and it is possible to attach the removed cells to the edge and remove the cells.
さ ら に 、 エ ッ ジ に付着 し た細胞 を ス ラ イ ド ガ ラ ス へ 塗抹 す る 場合 に は 、 擦 過部 の側面 を ス ラ イ ド ガ ラ ス に 押 し 付 け 、 採取器具 を 回転 さ せ る か 、 ま た は前方 へ摺 動 さ せ る ^: と に よ り 、 エ ッ ジ に付着 し た細胞 を 該 エ ツ ジ か ら 剝離 さ'せ て ス ラ イ ド ガ ラ ス 上 に 移す こ と 力 S で き ' る 。 In addition, when the cells adhered to the edge are smeared on the slide glass, press the side of the rubbed part into the slide glass and attach the collection instrument. Rotate or slide forward ^: to separate cells adhering to the edge from the edge and slide glass. You can move it up.
本 発 明 の 上 記 及 び 上 記 以 外 の 目 的 、 構 成及 び効 果 は 、 図面 を 参照 し て行 う 実施例 の 説明 に よ り 明 確 に す る 。 な お 、 以下 に 示す実施例 は単 に 好 ま し い例 で あ つ て 、 本発明 が こ れ ら の実施例 に 限定 さ れ る も の で な い こ と は レゝ ラ ま で も な い 。 図面 の 簡 単 な説明 The above and other objects, configurations and effects of the present invention will be clarified by the description of the embodiment with reference to the drawings. It should be noted that the embodiments described below are merely preferable examples, and it is to be noted that the present invention is not limited to these embodiments. No. Brief description of the drawings
第 1 図 は本発明 の一実施例 を 示す全 体 の平 面 図 、 第 2 図 は錐状擦 過部 の拡大 図 、 第 3 図 は 第 2 図 に お け る A - A 線での 断 面 図 、 第 4 図及 び第 5 図 は へ ら 状擦過 部 の拡大 図及び そ の側 面 図 、 第 6 図 は採取 し た細胞 の 塗抹方法 を 示す要部側面 図 、 第 7 図 〜 第 9 図 は は そ れ
ぞれ従来 の細胞採取器具 の平 面 図 で あ る 発明 を 実施す る た め の 最良 の 形態 FIG. 1 is a plan view of the whole showing one embodiment of the present invention, FIG. 2 is an enlarged view of a conical scraping portion, and FIG. 3 is a cross-sectional view taken along line AA in FIG. 4 and 5 are enlarged views of the spatula and its side view, and FIG. 6 is a side view of the main part showing the method of smearing the collected cells, and FIGS. 9 The figure shows The best mode for carrying out the invention, which is a plan view of a conventional cell collection device.
以下、 本発明 の細胞採取器具の実施例 を 図面 に 基づ い て詳細 に つ い て説明 す る 。 Hereinafter, embodiments of the cell collection device of the present invention will be described in detail with reference to the drawings.
第 1 図 に全体 を 示す採取器具 1 は 、 細径棒状 を な す ノヽ ン ド レ 2 の両端 に錐状擦過部 3 と へ ら状擦過部 4 と を 備 えた構成 を 有 し て お り 、 こ れ ら を 比較的柔軟 で弾 性変形可能 な合成樹脂 に よ っ て一体 に形成 し た も の で あ る 。 The sampling device 1 shown in its entirety in FIG. 1 has a configuration in which a cone-shaped scraping portion 3 and a spatular scraping portion 4 are provided at both ends of a nozzle 2 having a small-diameter rod shape. These are formed integrally from a relatively flexible and elastically deformable synthetic resin.
上記ハ ン ド ル 2 は 、 断面 を 円形 ま た は 多角形 と な す と 共 に 、 そ の表面 を 微細 な 凹凸 に よ て粗面 と な す こ と に よ り 、 持 ち 易 く 且 つ 操 作 し 易 い 構成 と な っ て お リ 、 し か も 、 操作時 に 不必要 に 変形す る こ と の な い 程 度 の 剐性 を 保持 し て い る 。 The handle 2 is easy to hold because the cross section is circular or polygonal and the surface is roughened by fine irregularities. The structure is easy to operate, but still retains a degree of flexibility that does not cause unnecessary deformation during operation.
上記錐状擦過部 3 は 、 子宮頸管内 の細胞 を 剝離 し て 採取す る た め の も の で あ っ て 、 上記ハ ン ド ル 2 の先端 に 弾性変形 を 容易 に す る た め の細径部 2 aを 介 し て形成 され 、 第 2 図及び第 3 図 に具体的 に 示す よ う な構成 を 備 え て い る 。 即 ち 、 こ の錐状擦過部 3 は 、 子宮頸管 に 痛 み を 伴 う こ と な く 挿入 し 得 る 程度 の太 さ に 形成 さ れ る と 共 に 、 挿入部位 の個人差 に対応 で き る よ う に 円錐 や 角錐 な ど の全体的 に 先細 り を な す錐状 に 形成 さ れ て ぉ リ 、 該錐状擦過部 3 の側面 に は 、 該擦過部 を取 リ 巻 く 螺旋状の エ ッ ジ 5 が形成 され て い る 。 こ の エ ツ ジ 5
'は 、 擦 過部 3 の側 面 に ハ ン ド ル 2 の基端側 を 向 い て 立 上が つ た鋸歯状 の突起 S を 蟓旋状 に 形成 し 、 該突起 S の 稜 部 に形成 し た も の で あ る 。 ま た 、 こ の錐状擦過部 3 の側 面 に は 、 該擦過部の铀線方 向 に延 び る 複数 の溝 7 が擦過部 3 の 回 り に 一定間隔 を お い て形成 さ れ 、 該 溝 Ί の溝縁 に 擦過部の周方 向 を 向 く エ ッ ジ 8 が形成 さ れ て い る 。 さ ら に 、 錐状擦過部 3 の先端 3 aは球状 に 形 成 さ れ 、 子宮頸管へ安全 に 挿入 し 得 る よ う に な つ て い る 。 The above-mentioned conical scraping part 3 is for separating cells in the cervix and collecting them, and is a thin part for facilitating elastic deformation at the tip of the above-mentioned handle 2. It is formed via the diameter portion 2a, and has a structure as specifically shown in FIGS. 2 and 3. That is, the conical abrasion portion 3 is formed to be thick enough to be inserted into the cervix without pain, and it is possible to cope with individual differences in the insertion site. The conical or pyramid-shaped taper is formed in a conical shape, such as a cone or a pyramid, and on the side surface of the conical scraping portion 3, a spiral is formed around the scraping portion. Edge 5 is formed. This edge 5 ', A saw-tooth-shaped projection S rising up toward the base end side of the handle 2 on the side surface of the scraping portion 3 in a spiral shape, and formed on the ridge portion of the projection S. It is what you do. In addition, a plurality of grooves 7 extending in the X-axis direction of the rubbing part are formed at regular intervals around the rubbing part 3 on the side surface of the conical rubbing part 3, An edge 8 is formed at the groove edge of the groove く so as to face in the circumferential direction of the rubbing part. Furthermore, the tip 3a of the conical abrasion 3 is formed in a spherical shape so that it can be safely inserted into the cervix.
上記細径部 2 aの柔 ら か さ の 度合 い は 、 錐状擦過部 3 を 子宮頸管内へ挿入す る 際 に は不必要 に 変形 す る こ と は な い が 、 '採取 し た細胞 を ス ラ，ィ ド ガ ラ 'ス へ塗抹 す る 際 に は 、 該擦過部 3 を ス ラ イ ド ガ ラ ス へ押 し付 け る こ と に よ つ て 第 6 図 に示す よ う に 湾 曲 さ せ得 る 程度が好 ま し い The degree of softness of the small-diameter portion 2a does not unnecessarily deform when the conical abrasion portion 3 is inserted into the cervix. As shown in FIG. 6, when the rubbing part 3 is pressed onto the slide glass when the rubbing is applied to the slide glass and the slide glass, as shown in FIG. Gulf is preferable to be able to bend
一方 、 ノヽ ン ド ル 2 の基端 に 形成 さ れた へ ら状擦過部 4 は 、 後膣円蓋部 ま た は 子宮膣部 の細胞 を採取 す る た め の も の で 、 一定 の幅 を も ち 且つ先端が円弧状 に形成 さ れた柔軟 な板状体 "を 備 え て い る 。 こ の板状体 4 aの 先端側 の 部分 の表裏面 に は 、 第 4 図及び第 5 図 か ら 明 ら か な よ う に 、 そ の幅方 向 に 長い 多数 の鋸歯状 の リ ブ 1 0がー定間隔 で形成 さ れ 、 こ れ ら の リ ブ 1 0に よ っ て ハ ン ドル 2 の先端側 を 向 く ェ ッ ジ 1 1が形成 さ れ て い る 。 ま た 、 上記板状体 4 aに は 、 そ の先端か ら エ ツ ジ形成部 分 4 bの 中 間位置 に かけ て細胞採取部位へ の フ イ ッ ト 性
を 高 め る た め の複数 の 切込 み 1 2が設け ら れ 、 該板状体 4 aに お け る エ ツ ジ形成部分 4 bは エ ツ ジ 非形成部分 4 cよ リ 薄肉 に形成 さ れ て い る 。 On the other hand, the spatula 4 formed at the proximal end of the node 2 is for collecting cells in the posterior fornix or uterine vagina, and has a certain width. And a flexible plate-shaped member having an arc-shaped tip at the top and bottom of the plate-shaped member 4a. As can be seen from the figure, a large number of saw-toothed ribs 10 long in the width direction are formed at regular intervals, and the ribs 10 are formed by these ribs 10. The edge 11 is formed so as to face the tip side of the dollar 2. Also, the plate-shaped body 4a is located at a position between the edge and the edge forming part 4b. To the cell collection site A plurality of cuts 12 are provided to increase the height of the edge, and the edge forming portion 4b of the plate-like body 4a is formed to be thinner than the edge non-forming portion 4c. It has been.
上記錐状擦過 部 3 及びへ ら 状擦過部 4 に おけ る エ ツ ジ 5 , 8 , 1 1の 高 さ は、 表層細胞か ら 基底細胞 ま で を 的 確 に し か も 間質 を 損傷す る こ と な く 採取 で き る よ う に す る た め 、 3 0〜 5 0 0 a の 範 囲 に 設定 す る の が 好 ま し く 、 中 で も 5 0〜 3 5 0 m の範囲が最 も 好 ま し い 。 The height of the edges 5, 8, and 11 in the conical scraping part 3 and the spatula-like scraping part 4 may damage the stroma even from the superficial cells to the basal cells. It is preferable to set the range from 30 to 500a, so that the sample can be collected without any trouble.The range is 50 to 350m in the middle. Is most preferred.
上記採取器具 1 を 形成す る 合成樹脂素材 と し て は 、 細胞の付着性が良 く 、 且つ ス ラ イ ド グ ラ ス への転移性 に勝れ る 合成樹脂が好 ま し く 、 例 え ばポ リ エ チ レ ン 、 ポ ' リ ブ ώ ビ レ ン 、 * ^酸 'ビ ニ ル共重合体、 ヱ チ レ ン ビニ ル ア ル コ ー ル共重合体 な ど の ボ リ ォ レ フ ィ ン 系樹脂、 ポ リ ス チ レ ン 、 A B S な ど の ボ リ ス チ レ ン 系樹脂 、 ボ リ ィ ミ ド 、 ォ レ フ ィ ン ビ ニ ル ア ル コ ー ル共重合体樹脂 等 で あ る 。 As a synthetic resin material forming the above-mentioned collection instrument 1, a synthetic resin which has good cell adhesion and is superior in transferability to slide glass is preferable, for example. For example, polyolefins such as polystyrene, polyvinylene, * ^ acid vinyl copolymer, polyethylenevinyl alcohol copolymer, etc. Polystyrene, Polystyrene, Polystyrene such as ABS, Polyimide, Polyvinyl Alcohol copolymer resin, etc. is there .
な お、 上記擦過部 3 , 4 に お け る 細胞の付着性 を よ り 向 上 さ せ る た め 、 そ れ ら の表面 を 全体 と し て微小 な 凹 凸 を 持つ粗面 に形成す る こ と も で き る 。 In order to further enhance the cell adhesion at the scraping portions 3 and 4, these surfaces are formed as a rough surface having minute concaves and convexes as a whole. You can do that too.
上述 し た細胞採取器具 1 に おけ る 錐状擦過部 3 で子 宮頸管内 の細胞 を採取す る と き は 、 該錐状擦過部 3 を 子宮頸管 の入 リ ロ に押 し 当 て 、 採取器具 1 を 回転 さ せ る こ と に よ り そ れ を 子宮頸管内 に ね じ 込み式 に挿入 す る 。 従 っ て 、 子宮頸管が狭小 で あ っ た リ 、 癌 な ど に よ り そ の部分が硬化 し て広が り に く く な っ て い る 場合 で
あ っ て も 、 錐状擦 過 部 3 は そ の 部 分 を 徐 徐 に 押 し 広 げ な 力 S ら 円 滑 に 挿入 さ れ る こ と に な リ 、 痛 み を 伴 っ た り ^ 入 部位 を 傷付 け る お そ れ が な い 。 When the cells in the cervix cervix are collected by the conical abrasion part 3 in the above-described cell collection instrument 1, the conical abrasion part 3 is pressed against the resection of the cervix and the collection instrument is pressed. Screw it into the cervix by rotating 1. Therefore, in cases where the cervix was narrow, cancer or other parts of the cervix were hardened and became difficult to spread. Even so, the conical abrasion portion 3 is gradually pushed down to the portion, and can be smoothly inserted with a widespread force S. There is no risk of damaging the site.
次 に 、 挿 入 し た 錐状擦 過 部 3 を 铀線方 向 に 引 き 抜 く こ と に よ り 、 螺 旋 状 の エ ッ ジ 5 で 子 宮頸管 の 内壁 の 細 胞 が搔 き 取 ら れ 、 採取 さ れ る 。 こ の と き 、 ハ ン ド ル 2 を 若 干 回 転 さ せ 、 溝縁 の エ ッ ジ 8 で細胞 を 搔 き 取 る よ う に すれ ば 、 子 宮頸管 の 内壁全周 に わ た っ て 確実 に 細 胞 を 採取 す る こ と が で き る 。 Next, by pulling out the inserted conical scraping portion 3 in the direction of the line, the spiral edge 5 removes the cells on the inner wall of the cervix cervix. Are collected and collected. At this time, if the handle 2 is slightly rotated, and the cells are removed by the edge 8 at the groove edge, the entire circumference of the inner wall of the cervix and cervix can be spread. The cells can be reliably collected.
こ の よ う に し て 採取 し た 細胞 を ス ラ イ ド ガ ラ ス 上 に 塗抹 す 時 は 、 第 6 図 に 示 す，よ う に 、 採 取器 具 1 の 先 端 が変形 す る 程 度 の 力 で錐 状擦 過 部 3 の 側 面 を ス ラ イ ド ガ ラ ス 1 5に 押 し 付 け 、 ハ ン ド ル 2 を 回 し な が ら 錐状 擦 過 部 3 を 前 方 に ス ラ イ ド さ せ る 。 こ れ に よ り 、 エ ツ ジ 5 , 8 に 付 着 し た細胞 が剝 離 し 、 ス ラ イ ド ガ ラ ス ί 5に 確実 に 塗抹 さ れ る 。 When the cells collected in this manner are spread on a slide glass, as shown in Fig. 6, the tip of the sampler 1 is deformed as shown in FIG. Press the side surface of the conical rubbing part 3 against the slide glass 15 with a slight force, and turn the conical rubbing part 3 forward while turning the handle 2. Let it slide. As a result, the cells adhered to the edges 5 and 8 are detached, and are reliably smeared on the slide glass 5.
ま た 、 へ ら 状擦 過部 4 に よ っ て 後膣 円 蓋部 ま た は 子 宮膣 部 の 細 胞 を 採取 す る と き は 、 該 へ ら 状擦 過部 4 に お け る エ ッ ジ 形成 部分 4 bを 採取 部位 に 押 し 当 て 、 そ の エ ツ ジ 1 1で細胞 を 搔 き 取 る よ う に す る 。 When the cells of the posterior fornix of the vagina or the vagina are to be collected by the spatula 4, use the spatula 4. The edge-forming portion 4b is pressed against the collection site, and the cells are removed by the edge 11 thereof.
へ ら 状擦 過 部 4 で採取 さ れ た 細胞 は 、 そ れ を ス ラ イ ド ガ ラ ス 1 5に 押 し 当 て 、 前 方 に ス ラ イ ド さ せ る こ と に よ っ て 該 ス ラ イ ド ガ ラ ス に 塗抹 す る こ と 力 S で き る 。
The cells collected at the spatula scraping part 4 are pressed against the slide glass 15 to slide the cells forward. S can be applied to slide glass.
Claims
1. 細径 状 を な すハ ン ド ル （ 2 ) の先端 に 、 細胞 を 搔 き 取 る た め の エ ツ ジ を 備 え た擦過 部 （ 3 ) を 形成 し て な る 細胞採取器具 に お い て 、 上記擦過 部 （ 3 ) を 全体 と し て 先細 り を な す錐状 に 形成 し 、 該錐状擦過部 （ 3 ) の 側面 に ハ ソ ド ル （ 2 ) の基端側 を 向 い て立上 が っ た鋸 歯 状 の突起 (6) を 螺旋状 に 形成す る と 共 に 、 該突起 （ S ) の稜部 に 上記 エ ツ ジ (5) を 形成 し た こ と を 特徵 と す る 細胞採取器具。 1. A cell harvesting instrument that forms a scraping part (3) at the tip of a small diameter handle (2) with an edge for removing cells. Here, the scraping portion (3) is formed into a tapered cone as a whole, and the side of the cone-shaped scraping portion (3) faces the base end of the nozzle (2). In addition to forming the raised serrated protrusion (6) in a spiral shape, the edge (5) is formed on the ridge of the protrusion (S). A cell collection device.
2. 八 ソ ド ル （ 2 ) の先端 に弾性変形 を 容易 に す る た め の細径部 （2a)を 介 レて上記錐状擦過部 （3) を 形成 し た と を 特徴 と す る 特許請求 の範囲 第 1 項記載の細胞 採取器具 2. The conical scraping part (3) is formed at the tip of the eight rods (2) through a small diameter part (2a) for facilitating elastic deformation. The cell collection device according to claim 1
3. 錐状擦過部 （ 3 ) の側面 に 、 '該擦過部 の 铀線方 向 に延び る 複数 の溝 （ 7 ) を 擦過部 の 回 リ に 一定 間隔 を お い て形成 し 、 該溝 （7) の溝緣 に擦過部 （3) の周方 向 を 向 く エ ツ ジ （8) を 形成 し て あ る こ と を 特徴 と す る 特許 請求の範 囲 第 1 項記載の細胞採取器具。 3. A plurality of grooves (7) extending in the direction of the 铀 line of the rubbing part are formed at regular intervals in the side of the conical rubbing part (3). The cell collection device according to claim 1, characterized in that an edge (8) facing the circumferential direction of the scraping portion (3) is formed in the groove (7). .
4. 状擦 過部 （3) の先端 （3a)を 球状 に 形成 し た こ と を 特徴 と す る 特許請求 の 範囲第 1 項記載の細胞採取 目 4. The cell collection eye according to claim 1, wherein the tip (3a) of the rubbing part (3) is formed in a spherical shape.
5. 錐状擦過部 （3) の 表面 を 粗面 と し た こ と を 特徴 と す る 特許請求 の範囲第 1 項記載 の細胞採取器具。 5. The cell collection device according to claim 1, wherein the surface of the conical scraping portion (3) is roughened.
6 ン ド ル （2) を 断面多角形状 に形成 し 、 そ の表
面 を 粗 面 と し た こ と を 特 徴 と す る 特 許請求 の 範 囲 第 1 項 記 載 の 細 胞採取 器 具 。 6 The handle (2) is formed into a polygonal cross section, and the The cell sampling device described in paragraph 1 of the scope of the patent claim, characterized in that the surface is roughened.
7. ハ ン ド ル （ 2 ) と 擦 過 部 （ 3 ) と を 合成樹脂 に よ り 一 体 に 形 成 し た こ と を 特 徴 と す る 特許請求 の 範 囲 第 1 項記載 の 細胞採取 器 具 。 7. The cell collection according to claim 1, characterized in that the handle (2) and the rubbing part (3) are integrally formed by a synthetic resin. Equipment.
8. エ ッ ジ （5 , 8) の 高 さ を 30〜 500 μ. 0 に 設定 し た こ と を 特徴 と す る 特許請求 の 範 囲 第 1 項 ま た は 第 3 項 記 載 の 細胞採取 器 具 。 8. The cell collection according to claim 1 or 3, wherein the height of the edge (5, 8) is set to 30 to 500 μ.0. Equipment.
-
-
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85322386A | 1986-04-17 | 1986-04-17 | |
| US853,223 | 1986-04-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1987006114A1 true WO1987006114A1 (en) | 1987-10-22 |
Family
ID=25315424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP1987/000238 WO1987006114A1 (en) | 1986-04-17 | 1987-04-15 | Apparatus for cell sampling |
Country Status (2)
| Country | Link |
|---|---|
| DE (2) | DE3790193T1 (en) |
| WO (1) | WO1987006114A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0533694A1 (en) * | 1990-05-07 | 1993-03-31 | Kelly Anne Hunter | Clinical appliance and handle. |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE511864C2 (en) * | 1998-04-01 | 1999-12-06 | Medscand Medical Ab | Spatula for sampling including perforations |
| FR2794355B1 (en) * | 1999-06-01 | 2003-05-16 | Serge Schlee | INSTRUMENT FOR CURETHING, ABSORPTION, ASPECTIZATION OR SAMPLING WITH BULB HEAD FOR NATURAL OR ARTIFICIAL CONDUIT |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5244099A (en) * | 1975-10-01 | 1977-04-06 | Milan Albert R | Uterine internal tissue sample collector |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1087951A (en) * | 1975-10-01 | 1980-10-21 | Albert R. Milan | Endometrial sampling instrument |
| US4027658A (en) * | 1975-12-01 | 1977-06-07 | Manly Ernest Marshall | Instrument for taking samples |
| GB2159420B (en) * | 1984-05-29 | 1988-05-11 | Medscand Ab | Endocervical sampling brush and smear method |
-
1987
- 1987-04-15 WO PCT/JP1987/000238 patent/WO1987006114A1/en active Application Filing
- 1987-04-15 DE DE19873790193 patent/DE3790193T1/de active Pending
- 1987-04-15 DE DE19873790193 patent/DE3790193C2/de not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5244099A (en) * | 1975-10-01 | 1977-04-06 | Milan Albert R | Uterine internal tissue sample collector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0533694A1 (en) * | 1990-05-07 | 1993-03-31 | Kelly Anne Hunter | Clinical appliance and handle. |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3790193T1 (en) | 1988-04-21 |
| DE3790193C2 (en) | 1990-06-28 |
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