WO1986003781A1 - Procede pour produire des alginates possedant des caracteristiques physiques ameliorees et leur utilisation - Google Patents
Procede pour produire des alginates possedant des caracteristiques physiques ameliorees et leur utilisation Download PDFInfo
- Publication number
- WO1986003781A1 WO1986003781A1 PCT/NO1985/000080 NO8500080W WO8603781A1 WO 1986003781 A1 WO1986003781 A1 WO 1986003781A1 NO 8500080 W NO8500080 W NO 8500080W WO 8603781 A1 WO8603781 A1 WO 8603781A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- alginates
- alginate
- physical properties
- preparation
- improved physical
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Definitions
- the present invention relates to the preparation of alginates having improved physical properties, especially with respect to the formation of gels having inorganic or polyvalent organic ions.
- Said modified alginates are intended to be used for immobilizing and encapsulating enzymes and/or cells for use in biotechnological processes .
- alginate gels as an immobilizing material has several deficiencies, two of them being:
- Calcium alginate gels are destabilized by compounds having affinity for calcium, for instance EDTA, citrate, lactate and phosphate, as well as high concentrations of
- + + ++ cations such as Na , K and Mg ;
- Alginates currently used have a high degree of chemical heterogenity and provide gels having pores of such great size that proteins - enzymes and other macro- molecules - can leach out, at the same time as the size distribution of the pores is difficult to control.
- Alginate is the most important structural poly- saccharide in marine brown algae and is used for several industrial purposes wherein the properties of the polymer are utilized as a polyelectrolyte - for instance for gel formation and thickening purposes - and also for its water and ion binding capacity.
- the purpose of the present invention is to prepare alginates having physical properties satisfying the requirements for increased gel strength and stability and better controllable pore size.
- alginate is a polyuronide built up from two uronic acids, viz., D-mannuronic acid ( ) and the C-5-epimer L-guluronic acid (G) . They are arranged in such fashion that the polymer is further built up from three types of sequence: (G)-rich sequences called G-blocks, (M)-rich sequences called M-blocks and alter ⁇ nating structure symbolized by ( G G G) .
- G-blocks G-blocks
- M-rich sequences called M-blocks
- alter ⁇ nating structure symbolized by ( G G G G) alter ⁇ nating structure symbolized by ( G G G G) .
- the alginate's ability to form a gel by ionic binding, and the properties of said gel depends both on the relative content of the two uronic acids and on the distribution of the guluronic acid units along the chain.
- a high content of (G) -blocks yields, for instance, an alginate with great gel-forming capacity, which, techni ⁇ cally seen, is a valuable property of the polymer.
- the present invention is based on the following:
- the alginate is synthesized in the alga as poly- mannuronic acid and is thereafter modified by an enzyme, mannuronan-C-5epimerase, which converts D-mannuronic acid residues into i-guluronic acid residues within the chain.
- an enzyme mannuronan-C-5epimerase, which converts D-mannuronic acid residues into i-guluronic acid residues within the chain.
- the invention relates to a process for producing alginates having improved physical properties such as increased gel strength, by using enzymatic modification on a polymeric level.
- the process is characterized in that alginates derived from brown algae or bacteria are inoculated with an enzyme preparation.
- an enzyme preparation is preferably used a C-5-epimerase preparation, more preferably an alginate lyase-free mannuronan-C-5-epimerase produced from the earth bacterium Azotobacter vinelandii.
- the present invention also comprises the use of the thus modified alginates for immobilizing enzymes, cell organelles and cells by entrapment in gels of alginate or alginate having suitable cations, as well as by immobi ⁇ lizing biocatalysts by encapsulation in alginate poly- cation microcapsules .
- the mannuronan-C-5-epimerase may be isolated from cultures from the earth bacterium Azotobacter vinelandii. which produces both alginate and epimerase extracellu- larily.
- the fact that the enzyme is extracellular is a great advantage in the isolation process, and it also indicates that the enzyme may function freely in solution independent on intracellular factors, which is favourable to a technical exploitation of the invention.
- Immobilized enzymes as catalysts has obtained still greater importance in industry and will, in the years to come, become one of the most important expansion areas for biotechnology. Immobilized enzymes are often more stable, but first and foremost, they are easier to handle than free, soluble enzymes and may be used in continuous processes.
- the cells may serve as carriers for a single enzyme, such that isolation of the enzyme is unnecessary before immobilizing, or several enzymes may also be used in the cell in order to catalyse multistep processes (for instance synthesis of hormones, proteins, etc.).
- the epimerization degree measured by means of high solution n.m. . -spectro- scopy, shows an increase of the guluronic acid content from 68% to 79% (see the Table) .
- composition and GG content of the polymer before and after enzymatic modification measured by 1 high solution H-n.m.r. -spectroscopy
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Ce procédé consiste à inoculer des alginates dérivés d'algues brunes ou de bactéries, avec une préparation d'enzyme telle qu'une préparation de mannuronane-C-5-épimérase à partir d'Azotobacter vinelandii. Les alginates modifiés sont utilisés pour immobiliser des enzymes, des organites cellulaires ou des cellules ainsi que pour la microencapsulation de biocatalyseurs.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO845059A NO845059L (no) | 1984-12-17 | 1984-12-17 | Fremgangsmaate for fremstilling av alginater med endrede fysikalske egenskaper samt anvendelse av disse alginater. |
NO845059 | 1984-12-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1986003781A1 true WO1986003781A1 (fr) | 1986-07-03 |
Family
ID=19887997
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/NO1985/000080 WO1986003781A1 (fr) | 1984-12-17 | 1985-12-16 | Procede pour produire des alginates possedant des caracteristiques physiques ameliorees et leur utilisation |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0204805A1 (fr) |
NO (1) | NO845059L (fr) |
WO (1) | WO1986003781A1 (fr) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988002758A1 (fr) * | 1986-10-17 | 1988-04-21 | Protan A/S | Modification d'alginates ou d'autres composes contenant de l'acide uronique par traitement avec du co2 |
WO1994009124A1 (fr) * | 1992-10-08 | 1994-04-28 | Pronova Biopolymer A.S | Composes d'adn comprenant des sequences codant la mannuronan c-5-epimerase |
FR2849056A1 (fr) * | 2002-12-19 | 2004-06-25 | Centre Nat Rech Scient | Mannuronane c5-epimerases d'algues brunes, procedes d'obtention et utilisations |
WO2005120589A2 (fr) * | 2004-06-14 | 2005-12-22 | Ntnu Technology Transfer | Nouvel agent de contraste |
WO2006051421A1 (fr) * | 2004-11-12 | 2006-05-18 | Fmc Biopolymer As | Alginates modifies, leurs procedes de production, et leur utilisation |
WO2008004890A2 (fr) | 2006-07-04 | 2008-01-10 | Spermvital As | Conservation et administration/libération contrôlée de spermatozoïdes |
WO2013076232A1 (fr) | 2011-11-24 | 2013-05-30 | Spermvital As | Procédés de préparation d'hydrogels en utilisant des enzymes lipases |
US9422373B2 (en) | 2011-06-02 | 2016-08-23 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
US9446168B2 (en) | 2010-06-07 | 2016-09-20 | Beta-O2 Technologies Ltd. | Multiple-layer immune barrier for donor cells |
US9540630B2 (en) | 2008-09-17 | 2017-01-10 | Beta O2 Technologies Ltd. | Optimization of alginate encapsulation of islets for transplantation |
WO2018104160A1 (fr) | 2016-12-05 | 2018-06-14 | Spermvital As | Composition à libération prolongée |
WO2019234010A1 (fr) | 2018-06-04 | 2019-12-12 | Spermvital As | Kit fonctionnalisé pour la préparation d'hydrogels |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU742434A1 (ru) * | 1978-10-11 | 1980-06-25 | Ленинградский ордена Трудового Красного Знамени институт текстильной и легкой промышленности им.С.М.Кирова | Способ получени иммобилизованной пероксидазы |
JPS5974984A (ja) * | 1982-10-21 | 1984-04-27 | Sumitomo Chem Co Ltd | 固定化酵素もしくは固定化微生物の製造方法 |
-
1984
- 1984-12-17 NO NO845059A patent/NO845059L/no unknown
-
1985
- 1985-12-16 WO PCT/NO1985/000080 patent/WO1986003781A1/fr unknown
- 1985-12-16 EP EP19860900291 patent/EP0204805A1/fr not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU742434A1 (ru) * | 1978-10-11 | 1980-06-25 | Ленинградский ордена Трудового Красного Знамени институт текстильной и легкой промышленности им.С.М.Кирова | Способ получени иммобилизованной пероксидазы |
JPS5974984A (ja) * | 1982-10-21 | 1984-04-27 | Sumitomo Chem Co Ltd | 固定化酵素もしくは固定化微生物の製造方法 |
Non-Patent Citations (14)
Title |
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BIOCHIM. BIOPHYS. ACTA, vol. 192, no. 3, 1969, pages 557 - 559 * |
CARBOHYD. RES., vol. 17, no. 2, 1971, pages 297 - 308 * |
CARBOHYD. RES., vol. 20, no. 2, 1971, pages 225 - 232 * |
CHEMICAL ABSTRACTS, vol. 101, 1984, Columbus, Ohio, US; abstract no. 166114U * |
CHEMICAL ABSTRACTS, vol. 72, 1970, Columbus, Ohio, US; abstract no. 75879P * |
CHEMICAL ABSTRACTS, vol. 75, 1971, Columbus, Ohio, US; abstract no. 564K * |
CHEMICAL ABSTRACTS, vol. 76, 1972, Columbus, Ohio, US; abstract no. 31806R * |
CHEMICAL ABSTRACTS, vol. 82, 1975, Columbus, Ohio, US; abstract no. 167310F * |
CHEMICAL ABSTRACTS, vol. 95, 1981, Columbus, Ohio, US; abstract no. 147178J * |
DATABASE WPI Derwent World Patents Index; AN 1981-13334D/08 * |
GUMS. STAB. FOOD IND. APPL. HYDROCOLLOIDS; PROC. INT. CONF. 2ND 1983, 1984, pages 523 - 528 * |
NIPPON SUISAN GAKKAISHA, vol. 47, no. 7, 1981, pages 889 - 893 * |
PATENT ABSTRACTS OF JAPAN * |
PROC. INT. SEAWEED SYMP. 7TH 1971, 1972, pages 491 - 495 * |
Cited By (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988002758A1 (fr) * | 1986-10-17 | 1988-04-21 | Protan A/S | Modification d'alginates ou d'autres composes contenant de l'acide uronique par traitement avec du co2 |
WO1994009124A1 (fr) * | 1992-10-08 | 1994-04-28 | Pronova Biopolymer A.S | Composes d'adn comprenant des sequences codant la mannuronan c-5-epimerase |
US5939289A (en) * | 1992-10-08 | 1999-08-17 | Pronova Biopolymer A.S. | DNA compounds comprising sequences encoding mannuronan C-5-epimerase |
KR100338997B1 (ko) * | 1992-10-08 | 2002-11-11 | 에프엠씨 바이오폴리머 아에스 | 만누로난씨(c)-5-에피머라제를암호화하는서열을포함하는디엔에이(dna)화합물 |
FR2849056A1 (fr) * | 2002-12-19 | 2004-06-25 | Centre Nat Rech Scient | Mannuronane c5-epimerases d'algues brunes, procedes d'obtention et utilisations |
WO2004065594A3 (fr) * | 2002-12-19 | 2005-04-07 | Centre Nat Rech Scient | Mannuronane c5-epimerases d’algues brunes, procedes d’obtention et utilisations |
WO2004065594A2 (fr) * | 2002-12-19 | 2004-08-05 | Centre National De La Recherche Scientifique (C.N.R.S.) | Mannuronane c5-epimerases d’algues brunes, procedes d’obtention et utilisations |
WO2005120589A3 (fr) * | 2004-06-14 | 2006-06-15 | Ntnu Technology Transfer | Nouvel agent de contraste |
WO2005120589A2 (fr) * | 2004-06-14 | 2005-12-22 | Ntnu Technology Transfer | Nouvel agent de contraste |
JP2008519595A (ja) * | 2004-11-12 | 2008-06-12 | エフエムシー バイオポリマー エイエス | 変性アルギネートおよびその製造方法および用途 |
WO2006051421A1 (fr) * | 2004-11-12 | 2006-05-18 | Fmc Biopolymer As | Alginates modifies, leurs procedes de production, et leur utilisation |
WO2008004890A2 (fr) | 2006-07-04 | 2008-01-10 | Spermvital As | Conservation et administration/libération contrôlée de spermatozoïdes |
US9540630B2 (en) | 2008-09-17 | 2017-01-10 | Beta O2 Technologies Ltd. | Optimization of alginate encapsulation of islets for transplantation |
US9446168B2 (en) | 2010-06-07 | 2016-09-20 | Beta-O2 Technologies Ltd. | Multiple-layer immune barrier for donor cells |
US10285949B2 (en) | 2011-06-02 | 2019-05-14 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
US9422373B2 (en) | 2011-06-02 | 2016-08-23 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
US11337930B2 (en) | 2011-06-02 | 2022-05-24 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
US10842753B2 (en) | 2011-06-02 | 2020-11-24 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
US10292936B2 (en) | 2011-06-02 | 2019-05-21 | Massachusetts Institute Of Technology | Modified alginates for cell encapsulation and cell therapy |
WO2013076232A1 (fr) | 2011-11-24 | 2013-05-30 | Spermvital As | Procédés de préparation d'hydrogels en utilisant des enzymes lipases |
US10041059B2 (en) | 2011-11-24 | 2018-08-07 | Spermvital As | Methods for the preparation of hydrogels |
US9578871B2 (en) | 2011-11-24 | 2017-02-28 | Spermvital As | Methods for the preparation of hydrogels using lipase enzymes |
WO2018104160A1 (fr) | 2016-12-05 | 2018-06-14 | Spermvital As | Composition à libération prolongée |
WO2019234010A1 (fr) | 2018-06-04 | 2019-12-12 | Spermvital As | Kit fonctionnalisé pour la préparation d'hydrogels |
US11957123B2 (en) | 2018-06-04 | 2024-04-16 | Spermvital As | Functionalized kit for preparing hydrogels |
Also Published As
Publication number | Publication date |
---|---|
NO845059L (no) | 1986-06-18 |
EP0204805A1 (fr) | 1986-12-17 |
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