WO1983003138A1 - Appareil de controle de la luminescence et assemblage injecteur pour celui-ci - Google Patents

Appareil de controle de la luminescence et assemblage injecteur pour celui-ci Download PDF

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Publication number
WO1983003138A1
WO1983003138A1 PCT/US1983/000290 US8300290W WO8303138A1 WO 1983003138 A1 WO1983003138 A1 WO 1983003138A1 US 8300290 W US8300290 W US 8300290W WO 8303138 A1 WO8303138 A1 WO 8303138A1
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WO
WIPO (PCT)
Prior art keywords
receptacle
shutter
cap
injector
rotatable
Prior art date
Application number
PCT/US1983/000290
Other languages
English (en)
Inventor
Inc. Diagnostic Sciences
Wyn Yoland Nielsen
Original Assignee
Diagnostic Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnostic Sciences Inc filed Critical Diagnostic Sciences Inc
Priority to AU13800/83A priority Critical patent/AU1380083A/en
Publication of WO1983003138A1 publication Critical patent/WO1983003138A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

Definitions

  • Luminescence is generally referred to as the light emitted by the glow worm, the firefly and certain varieties of fish and other marine life, including bacteria and plankton. It also includes light which is emitted from synthesized organic chemicals, such as lu inol, and it is more specifically referred to as biolu inescence or chemiluminescence, depending upon the source of the reacting materials.
  • the light is derived from a chemical, rather than a thermal reaction, without the necessity, as in the case of fluorescence, for excitation from an external source.
  • Light which is emitted is measured in units called photons by an instrument, called a photometer.
  • Bioluminescent reactions generally involve the use of an enzyme derived from firefly lanterns, termed “luciferase”, as a catalyst, and the reaction is an indication of the measure of adenosine triphosphate (ATP) which is a fundamental component of the energy cycle or of nicotinamide adene dinucleotide (NADH) , a substance which is vital to cell metabolism.
  • ATP adenosine triphosphate
  • NADH nicotinamide adene dinucleotide
  • ATP and NADH are present only in living cells, and therefore, they are accepted indicators of the presence or absence of life in a certain biological sample being tested. In medicine, the detection or measurement of ATP can provide a basis for the microbiologist to rapidly verify the presence of bacteria in urine, blood, or some other body fluid.
  • ATP is only present in living cells
  • detection of ATP may enable Blood Banks to assess the viability of the blood in their inventories.
  • the clinical chemist may use determinations of the amount of ATP in cells to detect disfunction of the cells, or may use the determination of the amount consumed in a reaction to derive other information, e.g., the serum level of drugs. Because NADH is a participant in several enzymatic reactions, the estimation of NADH by bioluminescenee measurement allows measurement of at least a dozen enzyme systems.
  • ATP determinations can provide information about relevant biological activity, e.g., the activity of degrading agents in sewage treatment, as well as other information about water quality.
  • Chemiluminescence can be used in immunoassays, which are medical diagnostic procedures based upon the science of immunology dealing with the interaction of antigens and antibodies.
  • the light-producing chemical may be attached, through a chemical process, to a specific antibody or antigen and thereby serve as a label to be read when the antigen-antibody complex is ultimately formed.
  • Test apparatus such as that disclosed in U.S. Patent No. 3,871,767, has certain limitations and some disadvantages, and more sophisticated and reliable test apparatus is desired. SUMMARY OF THE PRESENT INVENTION
  • Luminescence test apparatus which couples a holder with a photometer and its associated read-out, which bolder is designed to accommodate a cuvette of standard design.
  • the cuvette is manually loaded into the holder which incorporates a shutter mechanism that is designed to block passage of all light from reaching the photometer other than that created chemically in the cuvette during the time when it is open.
  • a movable hollow cylindrical shutter element rotates about a central axis in cooperation with a fixed shutter element.
  • the injection assembly is lowered into place to create a light-tight environment in the central region where the cuvette resides.
  • the injector for delivering the chemical reactants to the sample solution in the cuvette to be tested is protected by a surrounding, retractable guide.
  • a cap fits downward over a rounded upper edge of the rotatable shutter element and assures alignment of the injector with the holder.
  • the inwardly converging bottom surface of the guide engages the top of the cuvette, and the guide retracts upward against the pressure of a downwardly-biasing spring as the cap is seated in place ' on the holder.
  • a detent enters a groove in the undersurface of the cap and locks the injection assembly to the holder when the cap is rotated to the ready or TEST position opening the shutter.
  • the lower discharge end of the injector extends into the upper end of the cuvette, and the discharge outlet is aligned to direct liquid against the sidewall of the cuvette just above the surface level of the sample to initiate swirling and rapid, thorough mixing of the chemical reactants which are injected via a supply system connected to the upper end of the injector.
  • FIGURE 1 is a front perspective view of luminescence test apparatus embodying various features of the invention
  • FIGURE 2 is an enlarged sectional view taken along the central axis of the injection assembly and the holder, looking at the FIG. 1 apparatus from the front with the shutter closed, and diagrammatically illustrating certain other components of the apparatus
  • FIGURE 3 is an exploded perspective view of the shutter mechanism plus certain other components of the test apparatus shown in FIG. 1
  • FIGURE 4 is an enlarged fragmentary plan view showing the assembled components of FIG. 3;
  • FIGURE 5 is an enlarged sectional view taken generally along the line 5-5 of FIG. 4;
  • FIGURE 6 is an enlarged fragmentary view showing the initial mating of the cap with the top of the holder and the detent, as the injection assembly is lowered into place; and
  • FIGURE 7 is an enlarged sectional view similar to FIG. 2 showing the cap after rotation to the TEST position and the shutter open, with the cuvette in place and liquid being discharged;
  • FIGURE 8 is an enlarged fragmentary- sectional view taken generally along the line 8-8 of FIG. 7.
  • Luminescence test apparatus which includes a chassis 11 which surrounds the mechanical and electronic working components and which on its front face includes both a digital read-out 13 and a graphical read-out 15 which display the results of measurement of the luminescence reaching the photometer 17 through a double convex lens arrangement 18 which are both located within the chassis.
  • An opening in the rear left-hand portion of the upper panel of the chassis 11 provides clearance for the holder 19 which is disposed therebelow and extends upward just above the level of the upper panel.
  • the injection assembly 21 is disposed atop the holder 19 and may be manually removed and simply set to one side, or it may be -mounted by a suitable bracket on a vertical rod to allow it to be slid upward and downward and swung to one side to permit installation and removal of a sample-carrying cuvette 29.
  • a dispenser 23 of standard design preferably a motor-driven dispenser, is mated with a bottle of commercially available reagent, i.e., the enzyme
  • the dispenser 23 is ajustably set to dispense a precise amount of liguid in a desired short interval of time each time it is actuated. A manually operated dispenser may alternatively be used if it meets these specifications.
  • the dispenser 23 supplies the liquid through flexible tubing 27, which may be light-shielding Teflon tubing, that leads to the top of the injector assembly 21.
  • a round-bottomed tube of glass or plastic can be used as a receptacle or cuvette 29 to hold the liguid sample to be tested. It may be either transparent or translucent.
  • the elongated test tube 29 is of sufficient length to extend upward so that its upper end is easily accessible above the bottom of a well 31 that is provided at the upper end of a rotatable shutter element 33.
  • the holder 19, which incorporates the shutter mechanism, is supported within a base or block 35 which is in turn attached to the top panel of the chassis 11 by screws or the like extending through the holes 36 therein.
  • a fixed shutter 37 is mounted in the block 35 by screws extending up through its circular base 38 and includes an upstanding tubular portion 39 of circular cross-section, which defines the central axis of the shutter mechanism with which the injector assembly is coaxial when it is installed thereupon.
  • a short upstanding ring 40 is received in a complementary groove in the undersurface of the base 35 and blocks passage of light thereinto.
  • a port or opening 41 of generally rectangular shape is provided about midway between the top and bottom of the tubular section 39. The port 41 is cut to have dimensions intermediate the ports in the rotatable shutter 33 as explained hereinafter.
  • the fixed shutter 37 is installed in the block 35 so that the port 41 faces the screen of a side-window photo ultiplier tube which serves as the photometer 17 and which is located to the right thereof as one looks at it from the front of the chassis 11, as depicted in FIG. 2.
  • OMP shutter terminates in a flat bottom surface 45 which includes a tapped hole 47 located on the centerline constituting the central axis.
  • the rotatable shutter 33 is hollow having an elongated cavity 48 along its centerline extending downward from an enlarged region 49 at its upper end wherein the well 31 is formed.
  • the lower portion of the cavity 48 is formed by a lower hollow cylindrical section 51 which has an outer diameter just less than the inner diameter * of the tubular sidewall 39 of the fixed shutter within which it rotates.
  • the bottom of the rotatable shutter 33 has a central hole 52 through which a shoulder screw 53 or the like extends to enter the tapped hole 47 of the fixed shutter 37 to complete the subassembly of the two shutter elements.
  • Plastic bearing rings 54a and b (FIG. 7) surround the stem of the shoulder screw 53 above and below the bottom wall of the rotatable shutter element 33 and serve as thrust bearings.
  • the upper ring 54a may be a relatively rigid material, such as nylon, while the lower ring 54b is formed from Teflon or the like which undergoes cold flow when the screw 53 is tightened to seat the shoulder upon the bottom wall 45.
  • a liner 55 having an inner diameter just larger than the standard cuvette 29 lines the cavity 48 to collect any liquid which could inadvertently reach it.
  • the liner 55 is preferably formed from translucent but cloudy polypropylene to provide a light-diffusing effect.
  • An outer tubular skirt 56 is a part of the movable shutter and is spaced from the hollow cylindrical section 51 a distance just greater than the thickness of the fixed shutter tubular portion 39.
  • the outer skirt 56 not only assures the relative rotational compatibility of the fixed and movable shutter elements, but substantially increases the light- ightness of the overall subassembly without the necessity of achieving very close tolerances in manufacturing.
  • Aligned rectangular ports 57,59 are respectively formed in the outer skirt 56 and in the inner hollow cylindrical section 51 of the rotatable shutter. These ports are of different horizontal and vertical dimensions, as discussed hereinafter, with the interior port 59 being narrower than the port 57 in the outer skirt.
  • a short tubular collar 61 is preferably seated in a groove in the upper surface of the block 35, within which collar a lower intermediate portion of the enlarged region 49 of the rotatable shutter is rotatably received.
  • a notch 62 approximately semicircular in cross section, is cut in the undersurface and sidewall of the enlarged region - just above the intermediate portion, as best seen in FIG. 4.
  • a radially disposed pin 63 is mounted extending radially outward from enlarged upper region 49 which defines the well 31.
  • and - upstanding ring portion 64 of the base 35 may be provided which is received in a complementary recess in the undersurface of the rotatable shutter element (FIG. 7).
  • the injection assembly 21 terminates in a lower cap 65 which is sized to fit downward over the rounded upper end of the rotatable shutter 33.
  • a groove 67 is cut in the interior wall of the depending flange portion of the cap, and the cap is mounted by screws 69 extending upward through the cap into tapped holes in a main barrel 71 of the injector.
  • the barrel 71 has a central passageway 73 which is located on the central axis defined by the fixed shutter, and the central passageway narrows at a neck region 75 near the upper end thereof.
  • a long metallic injector needle 77 extends downward from a bushing 79 installed in the neck 75.
  • the length of the needle 77 is such that it extends out the bottom of the passageway 73, and the lower discharge end portion 81 of the needle, which is otherwise straight, is bent at an angle of between about 3° and about 10°, preferably about 5°, for about the last one-quarter inch, so that the discharge outlet at the bottom of the needle 77 will be directed at a specific location along the sidewall of the cuvette 29, as discussed in more detail hereinafter.
  • the relatively fragile lower needle tip is protected by an injector guide 83, which has an enlarged lower portion and a main cylindrical body that allows it to freely slide up and down in the barrel passageway 73.
  • An interior central channel 85 through the guide 83 is larger in diameter than the needle 77, and the lower portion of the channel 85 receives a length 87 of polytetrafluoroethylene (Teflon) tubing so as to assure a good sliding fit with the needle.
  • Teflon polytetrafluoroethylene
  • the central channel 85 of the injector guide 83 is enlarged further near its lower end where it surrounds the discharge tip 81 of the needle just above the point where it terminates in a frusto-conical bottom surface 89.
  • the inwardly converging disposition of this bottom surface 89 serves a centering function with the top of the cuvette 29, as described hereinafter.
  • the guide 83 is adapted to slide between an extended and a retracted position and is- biased to the downward extended position by a compression spring 91 which is located in the upper end of the barrel passageway 73 where it seats about the lower portion of the needle bushing 79 and bears against the upper end of the guide.
  • a split-ring clip 93 or the like is snapped into a groove in the outer wall of the guide 83, and the clip travels in an enlarged region in the upper section of the cap 65 that terminates in a ledge 95.
  • the guide 83 is in its fully extended position.
  • the Teflon tubing is connected to the short upper end of the needle 77 that extends upward from the needle bushing 79, using suitable ferrules 101 that are compressed in place by a compression nut 103 which is screwed downward into the internally threaded upper end of the barrel passageway 73 from the intermediate position shown in FIG. 2.
  • the barrel end cap 98 With the tubing 27 attached to the upper end of the needle, the barrel end cap 98, which has female threads at its lower end that mate with male threads on the upper end of the barrel 71, is screwed downward as indicated by the arrow in FIG.
  • the barrel 71, the barrel end cap 98, the Teflon tubing 27 and the strain relief 97, as well as the cap 65 and the shutter elements 33 and 37, are all made of opaque light-blocking material. Accordingly, the design is extremely light-tight so that any light which is emitted through the aligned shutter ports is assured to originate in the chemical luminescence-producing reaction taking place in the cuvette 29 and is not leakage into the apparatus through the injection assembly 21.
  • the cap 65 and the injection assembly are separable from the top of the shutter element 33 so that the well 31 at the upper end of the holder 19 is accessible, and in this LOAD position the rotatable shutter element 37 is positioned approximately 180° from the position wherein its ports are aligned with the port 41 in the fixed shutter.
  • the cuvette 29 carrying the liquid sample to be tested is then lowered into the well 31 until it is in place within the liner 55 in rotatable shutter 33 with its lower end located just below the level of the bottom edges of the rectangular ports in the shutter elements.
  • the interior cylindrical wall 107 of the depending flange portion of the cap 65 centers on the curved upper end of the rotatable shutter 33 to insure that the injection assembly 21 is aligned on the main centerline, and the frusto-conical surface 89 of the injector guide contacts the upper end of the cuvette 29 to achieve a dual function.
  • the upper surface of the groove 67 in the cap 65 engages the top surface of the head of a locking detent 109 that is mounted in a hole 111 in the top surface of the block 35.
  • the hole 111 is aligned at an angle of between about 10° and about 30°, preferably about 15°, from the vertical and is generally radial to the main centerline of the holder.
  • a compression spring 113 biases the detent upward and toward the rotatable shutter element where it is seated in the notch 62 therein in the LOAD position.
  • the pin 63 is received in a corresponding radial recess 117 cut in the undersurface of the cap 65.
  • Rotation of the cap 65 about 180° via a radially extending handle 119 turns the shutter mechanism to the fully open position, termed the TEST position, and the apparatus is ready to assay a sample.
  • the cap 65 stops precisely at the desired LOAD and TEST positions because a depending pin- 121 (FIG. 2) hits one or the other of two abutments 123 press fit into a circular groove 125 in the top surface of the block 35 just interior of the collar 61.
  • a synthetic rubber O-ring 126 serves as a bearing between the rotatable shutter element 33 and the base 35.
  • the discharge tip 81 of the needle is well below the upper end of the cuvette 29 so that it is ready for delivery of the chemical reagents. Because the notch 62 in the rotatable shutter is now displaced 180° from the detent 109 (see FIG. 4) , the cap 65 is locked to the holder 19, and potentially damaging ambient light cannot reach the sensitive electrode of the photometer 17.
  • Activation of the dispenser 23 causes a fixed quantity of liquid to flow through the Teflon tubing 27 down the needle 77 and to be discharged as a narrow stream from the port at the lower end of the needle in a given time interval, e.g., about one second.
  • the narrow stream of liquid being discharged downwardly and just slightly radially outwardly from the needle port impinges against the sidewall of the cuvette at a location just above the surface of the liquid sample contained therein.
  • the orientation is preferably such that the narrow stream impinges against a region of the sidewall that will be at or just below the final surface level in the cuvette after all of the reagent is dispensed.
  • Effective reproducible measurement of luminescence is dependent upon achieving prompt, thorough and consistent mixing because the rapidity of the reaction is such that measurement of peak luminescence may often occur a few seconds, e.g. 2 or 3, following the mixing of the reagents with the sample and sometimes within hundreds of milliseconds.
  • the apparatus is provided with circuitry that monitors the photomultiplier tube reading 20 times a second and automatically updates and continues to display the peak reading sensed until a set number of seconds, e.g. 2, expire without a further increase in the light reaching the photometer, at which time the read-out values are locked in.
  • the apparatus may employ cuvettes 29 in the form of 6 x 50 mm. clear glass tubes that are accommodated in a liner 55 disposed within a fixed shutter element 37 having an interior diameter of about one-half inch.
  • the port 59 in the interior hollow cylindrical portion of the rotatable "shutter 33 may be about 0.35 inch across, the port 41 in the fixed shutter may be about 0.45 inch wide, and the horizontal dimension of the port 57 in the outer skirt may be about 0.63 inch.
  • the discharge port of the needle 77 may be located about 5mm. into the cuvette 29, and the inner diameter of the needle should be between about 0.01 and 0.04 inch. The size is dependent upon the amount of liquid to be injected and the size of the cuvette 29, and an 0.016 I.D. needle is used to inject about 100 in a 6 x 50mm tube.
  • the size of the cuvette 29 - ' - ' is preferably chosen so that the final depth of the liquid is between about one and about one and one-half times the diameter of the cuvette to assure prompt thorough mixing occurs.
  • the shutter arrangement accomplishes the effective blocking of ambient light when it is in the closed positon without the use of pressure-bearing seals which are inherently subject to wear and surface imperfections and without the need to hold close manufacturing tolerences.
  • the baffling effect is such that ambient light photons entering the cavity 48 from the top when the cap 65 is removed must travel out through the port 59, then forward through the thin tubular passageway between the shutter elements 33, 37, out the port 41, and then back through the thin tubular passageway interior of the skirt 56 to the rearward-facing port 57. Any photons able to travel through this maze can be attenuated by a light-absorbing pad 128 optionally mounted in the block 35 opposite the port 57 of the closed shutter (FIG. 2) .
  • both the outer surface of the rotatable shutter 33, particularly in its enlarged upper portion 49, and of the corresponding regions at the top of the block 35 are employed to minimize the entry of ambient light through this region, and additional rings 64 can be provided if felt necessary for a particular test apparatus.
  • This ring 64, the upstanding collar 61 and an inwardly extending flange 130 are effective in shutting out the entry of ambient light.
  • the shutter mechanism has application in other chemical reaction measurement 5 apparutus wherein control of light-transmission is important.
  • the detent mechanism for securing a removable cap to a rotatable element is expected to have broader application to other test apparatus.

Abstract

Un appareil de contrôle de luminescence comprend des moyens photométriques (17) avec dispositif de lecture associé, un assemblage injecteur (21) et un récipient avec obturateur incorporé pour recevoir une cuvette (29) ou autre réceptacle contenant l'échantillon que l'on veut tester. L'assemblage injecteur comprend une enceinte cylindrique (71), un couvercle (65), un tube d'alimentation, un injecteur (77) et un guide protecteur environnant (83). Un mécanisme obturateur rotatif forme une partie du récipient et utilise des éléments obturateurs creux (33, 37), coaxiaux, conçus pour bloquer tout passage de lumière, sauf lorsque des orifices de passage (41, 57, 59) dans les éléments sont alignés. Après que la cuvette (29) portant l'échantillon a été placée axialement à l'intérieur des éléments obturateurs creux, l'assemblage injecteur (21) est baissé et mis en position. Le couvercle (65) s'adapte sur le sommet de l'élément obturateur mobile, et le guide protecteur (83) aligne l'extrémité supérieure de la cuvette dans l'axe et se retire en montant, de sorte que l'injecteur (77) s'étend dans la cuvette. L'injecteur (77) dirige du liquide contre la paroi latérale de la cuvette (29) à un endroit situé juste au-dessus du niveau de l'échantillon afin de produire un tournoiement et un mélange rapide et total. En conséquence, des résultats reproductibles de contrôle sont obtenus.
PCT/US1983/000290 1982-03-05 1983-03-04 Appareil de controle de la luminescence et assemblage injecteur pour celui-ci WO1983003138A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU13800/83A AU1380083A (en) 1982-03-05 1983-03-04 Luminescence test apparatus and injector assembly therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35496282A 1982-03-05 1982-03-05
US354,962820305 1982-03-05

Publications (1)

Publication Number Publication Date
WO1983003138A1 true WO1983003138A1 (fr) 1983-09-15

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WO (1) WO1983003138A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995024633A1 (fr) * 1994-03-11 1995-09-14 Tecator Ab Caisson d'analyse
EP0718622A2 (fr) * 1994-12-20 1996-06-26 Biosensor Laboratories Co., Ltd. Procédé et dispositif de mesure de la chimioluminescence
EP0753735A2 (fr) * 1995-07-13 1997-01-15 Ciba Corning Diagnostics Corp. Appareil pour tester un spécimen
EP0838678A1 (fr) * 1995-07-10 1998-04-29 Precision System Science Co., Ltd. Instrument de mesure
EP0984283A2 (fr) * 1991-03-04 2000-03-08 Ciba Corning Diagnostics Corp. Moyen d'isolation et procédé d'évacuation d'un liquide d'une cuvette
EP3056894A4 (fr) * 2013-10-07 2017-06-28 Universal Bio Research Co., Ltd. Dispositif de mesure de chimiluminescence et son procédé

Citations (5)

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US3707174A (en) * 1970-12-30 1972-12-26 Diehl Mateer Co G Apparatus for filling containers
US3991627A (en) * 1975-11-28 1976-11-16 Block Engineering, Inc. Alignment device for sample containers
US4099920A (en) * 1977-03-17 1978-07-11 Baxter Travenol Laboratories, Inc. Temperature control and stirring apparatus for luminescence measuring photometer
US4291230A (en) * 1979-03-07 1981-09-22 Baxter Travenol Laboratories, Inc. Fluorometric analyzer including shutter means for simultaneously shielding sample and photodetector during sample change
US4319842A (en) * 1980-07-17 1982-03-16 Baxter Travenol Laboratories, Inc. Photomultiplier protector for a fluorometer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3707174A (en) * 1970-12-30 1972-12-26 Diehl Mateer Co G Apparatus for filling containers
US3991627A (en) * 1975-11-28 1976-11-16 Block Engineering, Inc. Alignment device for sample containers
US4099920A (en) * 1977-03-17 1978-07-11 Baxter Travenol Laboratories, Inc. Temperature control and stirring apparatus for luminescence measuring photometer
US4291230A (en) * 1979-03-07 1981-09-22 Baxter Travenol Laboratories, Inc. Fluorometric analyzer including shutter means for simultaneously shielding sample and photodetector during sample change
US4319842A (en) * 1980-07-17 1982-03-16 Baxter Travenol Laboratories, Inc. Photomultiplier protector for a fluorometer

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0984283A2 (fr) * 1991-03-04 2000-03-08 Ciba Corning Diagnostics Corp. Moyen d'isolation et procédé d'évacuation d'un liquide d'une cuvette
EP0984283A3 (fr) * 1991-03-04 2000-05-31 Ciba Corning Diagnostics Corp. Moyen d'isolation et procédé d'évacuation d'un liquide d'une cuvette
WO1995024633A1 (fr) * 1994-03-11 1995-09-14 Tecator Ab Caisson d'analyse
EP0718622A2 (fr) * 1994-12-20 1996-06-26 Biosensor Laboratories Co., Ltd. Procédé et dispositif de mesure de la chimioluminescence
EP0718622A3 (fr) * 1994-12-20 1997-07-23 Bio Sensor Kenkyusho Kk Procédé et dispositif de mesure de la chimioluminescence
EP0838678A1 (fr) * 1995-07-10 1998-04-29 Precision System Science Co., Ltd. Instrument de mesure
EP0838678A4 (fr) * 1995-07-10 1999-04-28 Precision Syst Science Co Ltd Instrument de mesure
EP0753735A2 (fr) * 1995-07-13 1997-01-15 Ciba Corning Diagnostics Corp. Appareil pour tester un spécimen
EP0753735A3 (fr) * 1995-07-13 1997-05-07 Ciba Corning Diagnostics Corp Appareil pour tester un spécimen
US5837195A (en) * 1995-07-13 1998-11-17 Chiron Diagnostics Corporation Luminometer
EP3056894A4 (fr) * 2013-10-07 2017-06-28 Universal Bio Research Co., Ltd. Dispositif de mesure de chimiluminescence et son procédé

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