WO1983001687A1 - Electro-analyse par impulsion - Google Patents

Electro-analyse par impulsion Download PDF

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Publication number
WO1983001687A1
WO1983001687A1 PCT/AU1982/000175 AU8200175W WO8301687A1 WO 1983001687 A1 WO1983001687 A1 WO 1983001687A1 AU 8200175 W AU8200175 W AU 8200175W WO 8301687 A1 WO8301687 A1 WO 8301687A1
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WO
WIPO (PCT)
Prior art keywords
potential
period
cell
integration
current
Prior art date
Application number
PCT/AU1982/000175
Other languages
English (en)
Inventor
Scientific And Industrial Research ... Commonwealth
Original Assignee
Short, David, Leslie
Shell, Graeme, Stanley, Gibb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Short, David, Leslie, Shell, Graeme, Stanley, Gibb filed Critical Short, David, Leslie
Priority to AU90512/82A priority Critical patent/AU9051282A/en
Publication of WO1983001687A1 publication Critical patent/WO1983001687A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/49—Systems involving the determination of the current at a single specific value, or small range of values, of applied voltage for producing selective measurement of one or more particular ionic species

Definitions

  • This invention concerns electro-analysis. That is, it concerns measurement of the concentration of an electroactive material (termed an "analyte”) in an electrolyte.
  • the invention was developed primarily to measure the concentration of oxygen dissolved in water but, as will be seen, the invention is not limited to this application.
  • the apparatus currently used to measure the analyte concentration in an electrolyte consists of an electrolytic cell with electrical connections to an instrument which contains cell potential control circuitry and electrode current measuring means. That instrument provides an indication of the analyte concentration.
  • electrolytic cells that are suitable for these measurements are voltammetric and galvanic oxygen sensors, and dropping mercury electrode cells. Generally, those cells contain an indicator electrode and a counter electrode, and sometimes a third electrode which is used as a separate reference electrode. All the electrodes are in contact with the electrolyte under investigation.
  • Electrode current, which is related to analyte level in the vicinity of the indicator electrode, is that current which flows between the indicator electrode and the external circuit.
  • Cell potential (or indicator electrode potential) is the potential of the indicator electrode relative to whatever electrode is being used as a reference electrode.
  • the present invention although applicable to all types of electrolytic cells and sensors, has a particular application to the measurement of dissolved oxygen level (partial pressure) in still water. That measurement involves the use of membrane-covered oxygen sensors. Because that application of the present invention will be referred to frequently in this specification, it is convenient to use the terms "cathode” and “anode” for “indicator electrode” and “counter electrode”, respectively, when the measurement of dissolved oxygen is discussed.
  • pulse amperometr * sometimes called “chronoamperometry” or “pulse polarography”
  • Faradaic current that is, current related to oxygen concentration
  • Another pulse technique has been reported to produce low "zero” errors, but each measurement in that technique requires a large number of pulses, and the calibration of the instrument was reported to be non- linear.
  • This objective is achieved by integrating the cell current for a period which encompasses the time during which the applied pulse is "on” and at least part of the "off” time before the next pulse.
  • an instrument for measuring the concentration of electroactive material said instrument being adapted for use with an electrolytic cell and comprising means to apply sequentially to the cell an On-potential for an On-period of time and an Off- potential for an Off-period of time, characterised in that said apparatus includes a) integration means to integrate the cell current during the application of the On-potential and the Off-potential for a period of time comprising the On-period and at least part of the Off-period; b) control means to control the length of the On- period and the Off-period and to preselect the values of the On-potential and the Off- potential; and c) measuring means to display and/or record the integrated cell current.
  • the present invention also encompasses a method of electro-analysis to determine the concentration of electroactive material in an electrolytic cell, said method comprising sequentially applying an On-potential to the cell for an On-period of time and applying an Off-potential for an Off-period of time, characterised by the steps of a) selecting said On-potential to produce a measurable Faradaic cell current and selecting said Off-potential to produce zero Faradaic current in said cell; and b) integrating the total cell current during the application of the On-potential and the Off-potential for a period of time comprising the On-period and at least part of the Off-period, so that the capacitive component of the integral of the On-period cell current is cancelled by the integration of the Off-period cell current.
  • On- and Off-potentials, and the On- and Off-periods are the same criteria that are used in the existing pulse techniques (for example, pulse amperometry, pulse polarography or pulse coulometry) , with the exception that there is no need for capacitive current to be very low at the end of a pulse (that is, at the end of an
  • the On-potential is selected to produce a measurable Faradaic current while the Off-potential is selected to produce zero Faradaic current.
  • the electrode current is integrated over an integration
  • this integration period which comprises the total On-period and at least that part of the Off-period which is sufficient for cancellation of the capacitive component of the integral of the On-period electrode current. This is achieved if the integral of Off-period current is equal in magnitude but opposite in sign to the integral of On-period capacitive current.
  • the minimum value of this integration period depends on the type of electrolytic cell used in conjunction with the instrument, and also on the pulse regime employed by the instrument.
  • the present invention may be used in applications where the Faradaic Off-potential current is not zero and its integral is not small in relation to the On-period Faradaic current integral. Integration under these conditions would normally produce an error in the indication of the analyte level.
  • the instrument and method of the present invention may be used to cancel this error by instrument circuitry which provides a first integral for a first time period corresponding to an integration period as stated in the last preceding paragraph, and then subtracts from it a second integral produced by integrating the Off-period current for a second time period beginning after the capacitive component has decayed to an insignificant value. These two time periods are equal in length. Examples of instances where this approach will be adopted are the measurement of low analyte concentration and the cancellation of the effects of offset voltages or currents produced by the instrument electronic circuitry.
  • Figure 1 depicts a typical oxygen sensor voltammogram.
  • Figure 2 is a graph of cathode current versus time for pulsed operation of an electrolytic cell.
  • FIG. 3 is a timing diagram for the operation of the instrument of Figure 4.
  • Figure 4 is a block diagram of the preferred embodiment of the present invention for use in the analysis of the oxygen content of still water.
  • Figure 5 is a circuit diagram of one form of On/Off voltage source and switching circuit.
  • Figure 6 is a circuit diagram of an alternative means of providing the On/Off voltage.
  • Figure 7 is a circuit diagram of a digital integrator. DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS
  • Figure 1 shows a voltammogram obtained by plotting equilibrium cathode current against the cathode potential (relative to anode) for a constant oxygen level.
  • the voltammogram may conveniently be used to select the On- and Off-potentials for the present invention.
  • the potentials shown in Figure 1 as A and B, respectively, would be selected.
  • Figure 2 is a graphical representation of the cathode current that is produced by an electrolytic cell of which the cathode potential is controlled by the On/Off voltage source of the instrument of the invention.
  • line D represents the total electrode current including the Faradaic component and the capacitive component shown by line C.
  • the Off-period Faradaic current is practically zero (that is, it is very small compared with the On-period Faradaic current). Cancellation of the capacitive component may be achieved if the integration period begins at time Tl and ends at (or subsequent to) time T3.
  • the instrument depicted by the block diagram of Figure 4 includes a digital controller 10, one output of which is connected by line 30 to control a switched On/Off voltage source 13.
  • Source 13 sequentially applies preselected On and Off voltages to the anode of cell 14 via line 40.
  • the cathode current of cell 14 is connected to a current amplifier 16 via line 46.
  • Amplifier 16 has a voltage output which is proportional to the cathode current. This voltage is integrated by the integrator 18 and the integrated value is displayed by the readout device 20.
  • the digital controller 10 resets the integrator 18 via line 19 immediately prior to the beginning of each On-period and also provides signals via line 21 to the readout device at the end of the predetermined integration period to cause it to digitise (if necessary) and display the integrated current measurement.
  • the durations of the On- and Off-periods and the integration period are controlled by the digital controller according to a preselected program.
  • the On- and Off-potentials, applied by the switched On/Off voltage source 13, are also preselected by the operator of the instrument.
  • FIG. 5 One form of circuit that may be used for the switched On/Off voltage source 13 is shown in Figure 5.
  • this circuit there are an On-voltage source 38 and an Off-voltage source 52, with two electronic switch elements 32 and 34 arranged in series with the voltage sources 38 and 52 respectively.
  • the switch elements apply selected voltages via series resistor Rl, through a buffer amplifier circuit 44, to line 40, which is connected to the anode of the cell 14.
  • the control line 30 from the digital controller 10 is connected directly to switch 32 and is also connected, via inverter 36, to switch 34.
  • Switch 32 is normally off whilst switch 34 is normally on, so that, in the absence of a signal from the controller, the Off-voltage is fed to line 40 via the buffer amplifier 44.
  • inverter 36 Upon the receipt (through line 30) of a pulse from the controller 10 (the duration of which pulse is the On-period) , the action of inverter 36 ensures that switch 34 is turned off and switch 32 is turned on to connect the On-voltage source 38 via the buffer amplifier 44 to line 40.
  • FIG. 6 A second example of the switched voltage source 13 is shown in Figure 6.
  • This form of the source comprises a voltage reference 31, an amplifier 45 with series resistor 37 permanently connected and a resistor 35 connected during the On—period by switch 33.
  • the output of amplifier 45 is connected to line 40, which, (as already shown) is connected to the anode of the cell 14.
  • Control line 30 from the digital controller 10 is connected to switch 33, to which the reference voltage 31 is also connected.
  • Switch 33 is normally off " so that in the absence of a signal on line 30 from the controller, the amplifier 45 feeds the Off-voltage to line 40, the Off-voltage being set by series resistor 37 and feedback resistor 39.
  • resistor 35 Upon receipt of a pulse (through line 30) from the controller, the duration of which is the On-period, switch 33 is turned on, thereby connecting resistor 35 in parallel with series resistor 37. In this situation, amplifier 45 applies the On- voltage to line 40, the On-voltage being set by resistors 35 and 37 in parallel, together with feedback resistor 39. If a negative Off-voltage should be required (for example, for a galvanic oxygen sensor), resistor 37 will be connected to a different (positive) voltage source.
  • the On- and Off- voltages are applied sequentially to line 40, which is connected to the anode of cell 14.
  • the cathode of cell 14 is connected via line 46 to a virtual ground (zero volt) input of the current amplifier 16.
  • the On- and Off-voltages applied by the instrument are potentials of the anode relative to the cathode, these voltages being opposite in sign to the cathode potential shown in Figure 1.
  • the integrator 18 may be a simple analog- integrator.
  • the circuit shown in Figure 7 includes a digital integrator as an example of integrator 18.
  • the circuit of Figure 7 provides an up digital count for positive integrands and subtracts down counts from the. up count for negative integrands.
  • this circuit comprises a switchable unity gain circuit 58 which switches positive voltages on line 50 directl to line 51 and inverts negative voltages from line 50 to appear as positive voltages on line 51.
  • These voltages are presented to the input of a unipolar voltage-to- frequency converter 66 which provides, at line 67, a pulse output having a frequency which is proportional to the magnitude of the input voltage.
  • the up/down counter 68 converts the frequency input at line 67 into a digital count proportional to frequency.
  • the counter 68 is controlled by a signal from the digital controller introduced by line 30, which causes the counter to count up for positive voltages at line 50 and to count down for negative voltages at line 50.
  • the counter is reset by the digital controller via line 19 prior to the beginning of the next On-period.
  • the integrated count for this period is displayed in readout device 20, controlled by a signal on line 21.
  • FIG. 3 provides an illustration of the working of the instrument of the present invention. It contains various graphs of instrument and indicator electrode currents and voltages plotted against a common time events scale, to show the events controlled by the digital controller 10.
  • Graphs 40a and 46a of Figure 3 depict, respectively, the voltage applied to the anode and the corresponding cathode current.
  • the reset pulse of control signals 19 resets the integrator so that the integration period commences at the beginning of the On-period.
  • Graph 53 shows the output of integrator 18.
  • the end of the integration period is controlled from the digital controller by pulse on line 21 after the capacitive current has decayed.
  • Graphs 21a and 21b show the pulses on line 21 for the alternative integration periods, including part and nearly all of the Off- period respectively.
  • Graphs 54a and 54b show the corresponding integration periods.
  • the instrument described above was used with commercially available sensors to determine the level of oxygen dissolved in water.
  • Various time intervals for On-period and Off-period were tested and comparative tests were performed with an On-period of 1 second and an Off-period of 1 minute.
  • a galvanic sensor, Rexnord Model 62 (Rexnord Instrument Products, Malvern, Pennsylvania, USA) was used with the instrument and operated with cathode potential zero for the On-period and +0.7 volts for the Off-period.
  • a voltam etric sensor, IBC Model 526-001 International Biophysics Corporation, Irvine, California, USA was also used with the instrument, and it was operated at a cathode potential of -0.8 volts for the On-period and -0.2 volts for the Off-period. It was found that for the Rexnord sensor in air-saturated water, the cathode current, integrated in accordance with the present invention, had a capacitive component of only 5 percent of the integral of the On-period cathode current, yet for non-integrated current measurements at the end of the On-period, the capacitive component was approximately 90 percent of the total cathode current.
  • Another feature of the present invention is the reduction of the magnitude of the capacitive component of the electrode current that is achieved by adding to the electrolyte suitable capillary-active substances such as higher alcohols (for example, capryl alcohol) in order to reduce the amount of capacitive charge to be cancelled.
  • suitable capillary-active substances such as higher alcohols (for example, capryl alcohol)
  • capryl alcohol for example, capryl alcohol
  • a particularly beneficial feature of the present invention is that it may be used to measure oxygen level with a cathode material of high peroxide- decomposing ability such as silver or platinum.
  • the present invention reduces measurement errors caused by peroxide accumulation near the cathode.
  • the present invention is particularly useful in the measurement of oxygen levels in water.
  • the present invention may also be used for the measurement of the concentration of other analytes, when that measurement involves finding the Faradaic indicator electrode current which flows during a potential pulse in the electrolytic cell and there is a significant capacitive component at the end of the pulse, provided that a substantial proportion of this component is not due to changes in indicator electrode area during the integration period.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

La mesure de la concentration d'un matériau électro-actif (analyte) dans un électrolyte est effectuée en formant une cellule (14) de l'électrolyte et en appliquant des impulsions d'un potentiel de travail à l'anode de la cellule (14). Le courant traversant la cellule (14) est mesuré et intégré pendant le temps que le potentiel de travail est appliqué et pendant au moins une partie de la période de temps entre l'application des impulsions de potentiel de travail. Un contrôleur (10) commande la longueur de la période de travail et la fréquence de répétition des impulsions. Le courant de cellule intégré est affiché de préférence sous forme numérique par un dispositif d'affichage (20), pouvant également fournir un enregistrement de sa sortie. Une application typique de la présente invention est la mesure de la concentration de l'oxygène dans l'eau.
PCT/AU1982/000175 1981-10-29 1982-10-27 Electro-analyse par impulsion WO1983001687A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU90512/82A AU9051282A (en) 1981-10-29 1982-10-27 Pulsed electro-analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU133481 1981-10-29
AUPF1334811029 1981-10-29

Publications (1)

Publication Number Publication Date
WO1983001687A1 true WO1983001687A1 (fr) 1983-05-11

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PCT/AU1982/000175 WO1983001687A1 (fr) 1981-10-29 1982-10-27 Electro-analyse par impulsion

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EP (1) EP0091924A1 (fr)
WO (1) WO1983001687A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170998A2 (fr) * 1984-08-10 1986-02-12 Pacesetter AB Procédé pour la détection électrochimique de la concentration en oxygène
EP0269794A2 (fr) * 1986-11-07 1988-06-08 Environmental Technologies Group, Inc. Procédé d'opération d'une cellule électrochimique à laquelle un potential redox est appliqué
EP0581081A1 (fr) * 1992-07-15 1994-02-02 Prominent Dosiertechnik Gmbh Méthode pour la détermination des péracides
EP0636880A2 (fr) * 1990-07-20 1995-02-01 Matsushita Electric Industrial Co., Ltd. Appareil d'analyse quantitative
WO2007053191A2 (fr) * 2005-06-03 2007-05-10 University Of Texas System Électrochimie et chimioluminescence électrogénérée avec une électrode faradique unique
US7553448B2 (en) 2002-06-20 2009-06-30 Bioveris Corporation Electrochemiluminescence flow cell and flow cell components

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2766423A (en) * 1951-09-08 1956-10-09 Nat Res Dev Polarographs
GB1032231A (en) * 1962-01-30 1966-06-08 Sankyo Co Improved oscillographic polarograph
US3420764A (en) * 1964-03-16 1969-01-07 North American Rockwell Electrochemical analysis system
US4048041A (en) * 1976-05-24 1977-09-13 The United States Of America As Represented By The Secretary Of The Army Electronic system for providing specificity in an electrochemical analytical device
US4105523A (en) * 1976-03-31 1978-08-08 A. R. F. Products, Inc. Biochemical oxygen demand measuring device
US4260680A (en) * 1977-10-22 1981-04-07 Mitsubishi Chemical Industries, Ltd. Method and apparatus for the measurement of glucose content

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2766423A (en) * 1951-09-08 1956-10-09 Nat Res Dev Polarographs
GB1032231A (en) * 1962-01-30 1966-06-08 Sankyo Co Improved oscillographic polarograph
US3420764A (en) * 1964-03-16 1969-01-07 North American Rockwell Electrochemical analysis system
US4105523A (en) * 1976-03-31 1978-08-08 A. R. F. Products, Inc. Biochemical oxygen demand measuring device
US4048041A (en) * 1976-05-24 1977-09-13 The United States Of America As Represented By The Secretary Of The Army Electronic system for providing specificity in an electrochemical analytical device
US4260680A (en) * 1977-10-22 1981-04-07 Mitsubishi Chemical Industries, Ltd. Method and apparatus for the measurement of glucose content

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170998A2 (fr) * 1984-08-10 1986-02-12 Pacesetter AB Procédé pour la détection électrochimique de la concentration en oxygène
US4853091A (en) * 1984-08-10 1989-08-01 Siemens Aktiengesellschaft Method and apparatus for the electrochemical determination of oxygen concentration
EP0170998A3 (fr) * 1984-08-10 1989-08-30 Pacesetter AB Procédé pour la détection électrochimique de la concentration en oxygène
EP0269794A2 (fr) * 1986-11-07 1988-06-08 Environmental Technologies Group, Inc. Procédé d'opération d'une cellule électrochimique à laquelle un potential redox est appliqué
EP0269794A3 (fr) * 1986-11-07 1989-08-30 Environmental Technologies Group, Inc. Procédé d'opération d'une cellule électrochimique à laquelle un potential redox est appliqué
EP0636880A2 (fr) * 1990-07-20 1995-02-01 Matsushita Electric Industrial Co., Ltd. Appareil d'analyse quantitative
EP0636880A3 (fr) * 1990-07-20 1995-12-06 Matsushita Electric Ind Co Ltd Appareil d'analyse quantitative.
EP0581081A1 (fr) * 1992-07-15 1994-02-02 Prominent Dosiertechnik Gmbh Méthode pour la détermination des péracides
US7553448B2 (en) 2002-06-20 2009-06-30 Bioveris Corporation Electrochemiluminescence flow cell and flow cell components
WO2007053191A2 (fr) * 2005-06-03 2007-05-10 University Of Texas System Électrochimie et chimioluminescence électrogénérée avec une électrode faradique unique
WO2007053191A3 (fr) * 2005-06-03 2007-09-27 Univ Texas Électrochimie et chimioluminescence électrogénérée avec une électrode faradique unique
US8211279B2 (en) 2005-06-03 2012-07-03 Board Of Regents Of The University Of Texas System Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode
KR101311815B1 (ko) * 2005-06-03 2013-10-22 보드 오브 리전츠 오브 더 유니버시티 오브 텍사스 시스템 단일 패러데이 전극에서의 전기발생 화학발광 및 전기화학
KR101385384B1 (ko) * 2005-06-03 2014-04-14 보드 오브 리전츠 오브 더 유니버시티 오브 텍사스 시스템 단일 패러데이 전극에서의 전기발생 화학발광 및 전기화학
US8840774B2 (en) 2005-06-03 2014-09-23 Board Of Regents Of The University Of Texas System Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode

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