WO1982002696A1 - Dispositif gravimetrique de dilution - Google Patents

Dispositif gravimetrique de dilution Download PDF

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Publication number
WO1982002696A1
WO1982002696A1 PCT/US1982/000144 US8200144W WO8202696A1 WO 1982002696 A1 WO1982002696 A1 WO 1982002696A1 US 8200144 W US8200144 W US 8200144W WO 8202696 A1 WO8202696 A1 WO 8202696A1
Authority
WO
WIPO (PCT)
Prior art keywords
diluent
container
weight
sample
dilution factor
Prior art date
Application number
PCT/US1982/000144
Other languages
English (en)
Inventor
Systems Inc Spiral
Jeptha E Campbell
Samuel Schalkowsky
Donald Whitley
James E Gilchrist
Original Assignee
Systems Inc Spiral
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/232,531 external-priority patent/US4350186A/en
Priority claimed from US06/232,525 external-priority patent/US4345628A/en
Application filed by Systems Inc Spiral filed Critical Systems Inc Spiral
Priority to DE823231634T priority Critical patent/DE3231634T1/de
Publication of WO1982002696A1 publication Critical patent/WO1982002696A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00207—Handling bulk quantities of analyte
    • G01N2035/00217—Handling bulk quantities of analyte involving measurement of weight

Definitions

  • the invention relates to apparatus for making dilutions of samples of any weight with a diluent to a predetermined weight percentage of diluent.
  • liquid diluent In the fields of microbiology, biochemistry and chemistry it is often necessary to dilute liquid or solid samples with a diluent to a predetermined concentration by weight.
  • the liquid diluent may be used to make a slurry of the solid in the liquid. Bacterial density in solid food or fruit juices is determined by analysis of dilutions of these samples. In certain areas such as clinical laboratories or quality control laboratories it is necessary to make dilutions of large numbers of samples. While it is possible to manually dilute samples with diluents to a predetermined percentage by weight or volume, the amount of time required can be great where high accuracy or large numbers of dilutions are required.
  • the invention is an apparatus for diluting liquid or solid samples to a predetermined concentration by weight of a diluent.
  • the invention has means for measuring the weight of the container; means for specifying a predetermined dilution factor f by weight of a diluent in a sample; means for calculating TW - W, fluid conducting means disposed between a reservoir of diluent and the container for permitting the diluent to flow from the reservoir to the container for the purpose of diluting a sample within the container to the predetermined concentration by weight of diluent f; and selectively closeable valve means in fluid communication with the fluid conducting means which is disposed between the reservoir and the container for interrupting the flow of diluent between the reservoir and the container, said valve means.
  • the valve means is pulsed on and off when TW - W ⁇ 1 to prevent overshoot in the amount of diluent added to the sample for any value of f.
  • the invention also includes means for specifying a maximum weight limit UL of the combined weight of sample and diluent which is acceptable for a given dilution factor f and means for specifying a minimum weight limit LL of the combined weight of sample and diluent which is acceptable for a given dilution factor f.
  • Figure 1 is a system schematic of one embodiment of the present invention.
  • Figure 2 is a flow chart of the preferred form of a microprocessor control for the embodiment of Figure 1.
  • FIG. 3 is a system schematic of another embodiment of the presentinvention.
  • FIG. 1 is a general system schematic of the present invention 10 which functions to automatically dilute a sample 11 contained within a container 20 to a predetermined weight dilution factor f of a diluent 36.
  • dilutions of samples 11 are made without requiring measurement of the sample weight prior to placement in the container 20.
  • the dilutions made by the present invention are by weight percentage of the diluent in the sample 11.
  • a scale 12 with a TARE control lh which has an output line 16 on which is continually produced a signal of the weight placed on the pan 18 in binary coded decimal (BCD) format.
  • the TARE control 14 is activated is activated to produce a zero weight BCD output signal on line 16 when the pan 18 has a container 20 resting on it within which a sample 11 will be placed which is to be diluted by diluent 36.
  • Scales are commercially available having TARE capability and a BCD output which may be used with the present invention is a model PS15 which is manufactured by the Mettler Instrument Corporation of Hightstown, New Jersey.
  • the controller 22 of the present invention includes a plurality of controls which include a dilution factor selector control 24, an automatic dispenser switch 26, a rapid manual dispenser switch 32 which is used to manually open a first rapid dispense valve 34 to the flow of diluent 36 from reservoir 38 through conduit 40, under the power of pump 42, a slow dispense switch 44 which is used to manually open a second valve 46 to the flow of diluent 36 from a reservoir 38 through conduit 40 under the power of pump 42, a ready light 46A, an expensive sample indicator light 48, an insufficient sample indicator light 50, a power on indicator light 52, maximum weight control 53 and minimum weight control 55.
  • a dilution factor selector control 24 controls which include a dilution factor selector control 24, an automatic dispenser switch 26, a rapid manual dispenser switch 32 which is used to manually open a first rapid dispense valve 34 to the flow of diluent 36 from reservoir 38 through conduit 40, under the power of pump 42, a slow dispense switch 44 which is used to manually open
  • the maximum weight control 53 specifies the maximum weight of sample plus diluent which is permissible for the dilution of a sample to a specified dilution factor f.
  • the minimum weight control 55 specifies the minimum weight of sample plus diluent which is permissible for the dilution of a sample to a specified dilution factor f .
  • the rapid manual dispense switch 32 and the slow manual dispense switch 44 may be used to manually override the automatic control of valves 34 and 46 by the controller 22 to permit selective flow control of diluent into container 20.
  • the first valve 34 has a rated flow rate for a given pressure which is 10 times greater than the flow rate of the second valve 46.
  • the controller 22 has an interlock line 54 which is coupled to a switch 54A and attached to the chassis of the scale 12 which allows valves 34 and 46 attached to the horizontal member 76 to operate when the stanchion 74 is rotated so that switch 54A is closed and valve 34 and valve 46 are over the container 20, a power line 56 which provides suitable electrical power for the scale 12 and a pump activation line 58 which controls the activation of pump 42 for pumping diluent 36 from reservoir 38 through conduit 40, through either the first valve 34 or the second valve 46 into container 20 in a manner to be hereinafter described.
  • the power line for the controller 22 has been omitted.
  • the conduit 40 contains a T section having an input which is coupled to the pump 42 and first and second valves 34 and 36.
  • Valve control line 64 is connected between the controller 22 and the first valve 34 for selectively controlling the flow of diluent 36 from the pump 42 through the first valve in a manner to be hereinafter explained in conjunction with Figure 2.
  • Valve control line 66 is connected between the controller 22 and the second valve 46 for selectively controlling the flow of diluent 36 from the pump 42 through the second valve 46 in a manner to be hereinafter explained in conjunction with Figure 2.
  • the vavles 34 and 46 may have solenoids which are activated under the control of activation signals from the controller 22 over control lines 64 and 66.
  • Valves 34 and 46 may be model V5 valves sold by Skinner Precision Industries, Inc. of New England, Connecticut.
  • Bacterial filters 68 may be coupled between the outputs 70 and 72 of the first and second valves 34 and 46 to filter the diluent 36 prior to discharge into container 20.
  • the valves 34 and 46 are supported above container 20 by a metallic stanchion 74 and a horizontal member 76.
  • Figure 2 illustrates a flow chart of the preferred form of implementation of the controller 22 for the invention which utilizes a microprocessor to control the system operation.
  • a the starting point 200 of the microprocessor control program it is assumed the following sequence of events has occured: maximum and minimum weight UL and LL of samples and diluent have been entered; the dilution factor f has been set; the power has been turned on; a sample container 20 has been placed on the pan 18; the TARE control 14 has been depressed, a sample 11 has been placed in the container 20; and the automatic dispense switch 26 has been depressed.
  • the program proceeds to block 202 where the ready light on the controller is turned off to signify entry into a dilution of a sample.
  • the program proceeds to block 204 where the weight W of the sample to 0.1g in BCD format is read from the scale 12.
  • the program proceeds to block 206 where the weight W is stored in memory.
  • the program then proceeds to block 208 where the dilution factor f is read from the dilution input factor control 24 within the controller 22.
  • the program then proceeds to block 210 where the dilution factor f is stored in memory.
  • the program then proceeds to block 212 where the maximum weight limit UL of the sample and diluent is read from the maximum weight control 53 in the controller 22.
  • the range of the maximum weight linit is 0 ⁇ .UL-49999 grams.
  • the program then proceeds to block 214 where the maximum weight UL is stored in memory.
  • the program then proceeds to block 216 where the minimum weight limit LL of sample and diluent is read from the minimum weight control 55 in the controller 22.
  • the range of the minimum weight limit is 0 ⁇ LL ⁇ 9999 grams.
  • the program proceeds to block 218 where the minimum weight LL is stored in memory.
  • the program then proceeds to block 222 where TW> is stored in memory.
  • the program then proceeds to the decision point 224 where a determination is made if TW> UL. If the answer is "yes” the program branches to block 226 where an overweight light 48 in the controller 22 is turned on to indicate that the weight of the sample plus the diluent for achieving the previously set dilution factor f is greater than the. specified maximum weight which has been set with the maximum weight control 53.
  • the program then proceeds to block 228 where the memory elements are reset with switch 38 in the controller 22 to prepare the system for another dilution.
  • the program then proceeds to stopping point 230 which terminates all activity in the program. If the answer is "no" at decision point 224, the program branches to block 234 where the insufficient indicator light 50 in the controller 22 is turned on to indicate that the weight of the sample plus the diluent for achieving the previously set dilution factor f is less than the specified minimum weight which has been set with the minimum weight control 55.
  • the program then proceeds to block 236 where the memory elements in the controller 22 are reset with switch 28 to prepare the system for another dilution.
  • the program then proceeds to stopping point 238 which terminates all activity in the program.
  • the program proceeds to block 240 where the pump 42 is turned on and the valve 34 opened. At this point, the pump is pumping diluent 36 into the container at the higher rate of flow permitted by valve 34 than is possible with valve 46.
  • the program then proceeds to block 242 where the BCD output from scale 12 of the combined weight of diluent and sample is read.
  • the program then proceeds to decision point 244 where a determination is made if TW - W >10. If the answer is "no” the program loops back to block 242 and to decision point 244 where the calculation TW - W ⁇ 10 is made. As long as TW - W>10, valve number 34 is open and the highest flow rate of diluent into the container takes place.
  • the program then proceeds to block 248 where the weight W of sample plus diluent is read from the scale 12.
  • the program then proceeds to decision point 250 where a determination is made if TW - W ⁇ 1. If the answer is "no”, the program loops back to block 248. If the answer is "yes”, the program proceeds to block 252 where the valve is pulsed to be on for 0.2 seconds and pulsed off for 1.8 seconds.
  • the program proceeds to block 254 where the weight of the sample plus diluent is read from scale 12.
  • the program then proceeds to block 260 where the pump 42 is turned off, the ready light in the controller 22 is lit and the memory elements of the controller are reset.
  • FIG 3 is a general system schematic of the second embodiment 300 of the present invention.
  • the second embodiment is identical to the first embodiment of the present invention which is illustrated in Figure 1 with the exception that the apparatus for controlling the flow of diluent is different. Identical parts in Figures 1 and 4 are identified by identical reference numerals.
  • the flow of diluent between the reservoir and the container is controlled by the starting and stopping of a pump 302 without the first and second valves 34 and 46 of Figure 1.
  • the pump may be any type of pump which stops the pumping of fluid immediately when a stop command is applied thereto on lines 304 and/or 306 from controller 22. There are many types of pumps which have this capability.
  • the preferred type of pump is a peristaltic pump which may be a model Master Flex Pump C-7500-00 manufactured by the Cole-Parmer Corporation of Chicago, Illinois.
  • the peristaltic pump is preferred because it may be controlled to immediately cease the pumping of fluid.
  • reciprocating pumps, centrifugal pumps, rotary pumps, or other types of pumps which have a relatively high inertia during pumping would not be useful unless they contained valving which permitted the immediate stopping of the flow of liquid upon the application of the stop command from the controller 22.
  • the second embodiment may include a pump 302 which may be controlled to pump at two different flow rates which are used such that the higher flow rate is used when the quantity TW - W >10 and the lower flow rate is used when
  • the maximum and minimum weights UL and LL have been previously set by the maximum and minimum weight controls 53 and 55 of the controller 22 and are assumed to not warrant system shutdown.
  • the dilution factor f is set at 0.5 by the dilution factor selector control 24.
  • the sample of ethanol is then placed in the container 20 and the automatic dispenser switch 26 -is depressed.
  • the means for calculating TW reflects that the combined weight of ethanol and water. to achieve an 0.5 dilution factor is 200 grams.
  • the pump 42 is immediately activated and rapid dispense valve 34 is opened. The pump then proceeds to pump water into the container 20 through valve 34 until the combined weight of the water plus ethanol reaches 190 grams at which point the program causes rapid dispense valve 34 to close and slow dispense valve 46 is opened to reduce the rate of introduction of water into the container 20.
  • the valve 46 is repeatedly pulsed on for 0.2 seconds and off for 1.8 seconds.
  • the pump 42 continues to pump water into the container through the pulsating valve 46 until the weight of the water plus ethanol equals 200 grams which is equal to the previously calculated TW. The pump 42 is then shut off and valve 46 is closed to complete the dilution.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • External Artificial Organs (AREA)

Abstract

Dispositif (10) permettant d'obtenir des dilutions d'un echantillon (11) avec un diluant (36) jusqu'a concurrence d'un facteur de dilution f predetermine en poids du diluant. Une balance (12) pourvue d'une fonction TARE produit un signal de sortie du poids W d'un echantillon a l'interieur d'un recipient (20) en soustrayant le poids du recipient, Une commande (24) de facteur de dilution est prevue pour determiner le facteur de dilution f predetermine. Des blocs (218 et 220) sont prevus pour calculer et stocker la valeur TW = W/f qui est egale au poids total de l'echantillon plus le poids du diluant necessaire pour diluer l'echantillon jusqu'a concurrence du facteur de dilution f predetermine. Une pompe (42) est connectee entre un reservoir (38) de diluant et le recipient pour l'echantillon et sert a pomper dans le recipient la quantite de diluant necessaire pour diluer l'echantillon jusqu'a concurrence du facteur de dilution f en poids predetermine. La balance fournit une sortie continue du poids W de l'echantillon et du diluant a l'interieur du recipient a chaque instant. Un point de decision (256) est prevu pour determiner le moment ou TW - W = 0. Dans un mode de realisation, l'ecoulement du diluant vers le recipient est arrete par la fermeture d'une paire de vannes (34 et 46) situees entre la pompe et le recipient lorsque TW - W = 0.
PCT/US1982/000144 1981-02-09 1982-02-04 Dispositif gravimetrique de dilution WO1982002696A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE823231634T DE3231634T1 (de) 1981-02-09 1982-02-04 Gravimetrische verduennungsvorrichtung

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US232531810209 1981-02-09
US232525 1981-02-09
US06/232,531 US4350186A (en) 1981-02-09 1981-02-09 Gravimetric diluter
US06/232,525 US4345628A (en) 1981-02-09 1981-02-09 Gravimetric diluter

Publications (1)

Publication Number Publication Date
WO1982002696A1 true WO1982002696A1 (fr) 1982-08-19

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ID=26926083

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1982/000144 WO1982002696A1 (fr) 1981-02-09 1982-02-04 Dispositif gravimetrique de dilution

Country Status (5)

Country Link
EP (1) EP0070893A4 (fr)
JP (1) JPS58500337A (fr)
DE (1) DE3231634T1 (fr)
GB (1) GB2108288B (fr)
WO (1) WO1982002696A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2579749A1 (fr) * 1985-03-28 1986-10-03 Serg Gts Dispositif automatique de dilution, par prelevement permanent d'echantillons liquides a diluer et par dilution en discontinu
EP0651306A1 (fr) * 1993-10-25 1995-05-03 Alfatech S.R.L. Appareil pour préparer des solutions
US8459129B2 (en) 2008-05-30 2013-06-11 Stéphane Seznec Device for diluting of a sample

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2193581B (en) * 1986-07-03 1990-09-19 Francis Packaging Ltd Fluid dispensing apparatus and method of operation thereof
US8576217B2 (en) 2011-05-20 2013-11-05 Ignis Innovation Inc. System and methods for extraction of threshold and mobility parameters in AMOLED displays
CA2669367A1 (fr) 2009-06-16 2010-12-16 Ignis Innovation Inc Technique de compensation pour la variation chromatique des ecrans d'affichage .
US8803417B2 (en) 2009-12-01 2014-08-12 Ignis Innovation Inc. High resolution pixel architecture
CA2687631A1 (fr) 2009-12-06 2011-06-06 Ignis Innovation Inc Mecanisme de commande a faible puissance pour applications d'affichage

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3254728A (en) * 1964-03-11 1966-06-07 Howe Richardson Scale Co Automatic batch weigher using digital count-down control system
US3263761A (en) * 1962-03-16 1966-08-02 Lucas Industries Ltd Apparatus for dispensing predetermined weights of a plurality of different liquid orgranular materials
US3398689A (en) * 1966-01-05 1968-08-27 Instrumentation Specialties Co Apparatus providing a constant-rate two-component flow stream
US3516506A (en) * 1967-09-18 1970-06-23 Auto Control Inc Control apparatus for automatic industrial operations
US3528518A (en) * 1967-08-03 1970-09-15 Howe Richardson Scale Co Automatic batch weigher
US3805141A (en) * 1973-01-08 1974-04-16 United Aircraft Corp Bimodal inverter
US3959636A (en) * 1975-03-10 1976-05-25 Mangood Corporation Batching system
US4222448A (en) * 1979-06-29 1980-09-16 Owens-Corning Fiberglas Corporation Automatic batch weighing system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS506994A (fr) * 1973-05-25 1975-01-24
JPS5176664A (en) * 1974-12-26 1976-07-02 Daiju Doro Kensetsu Kk Nishunoekijotaino kongonodochoseihoho

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3263761A (en) * 1962-03-16 1966-08-02 Lucas Industries Ltd Apparatus for dispensing predetermined weights of a plurality of different liquid orgranular materials
US3254728A (en) * 1964-03-11 1966-06-07 Howe Richardson Scale Co Automatic batch weigher using digital count-down control system
US3398689A (en) * 1966-01-05 1968-08-27 Instrumentation Specialties Co Apparatus providing a constant-rate two-component flow stream
US3528518A (en) * 1967-08-03 1970-09-15 Howe Richardson Scale Co Automatic batch weigher
US3516506A (en) * 1967-09-18 1970-06-23 Auto Control Inc Control apparatus for automatic industrial operations
US3805141A (en) * 1973-01-08 1974-04-16 United Aircraft Corp Bimodal inverter
US3959636A (en) * 1975-03-10 1976-05-25 Mangood Corporation Batching system
US4222448A (en) * 1979-06-29 1980-09-16 Owens-Corning Fiberglas Corporation Automatic batch weighing system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2579749A1 (fr) * 1985-03-28 1986-10-03 Serg Gts Dispositif automatique de dilution, par prelevement permanent d'echantillons liquides a diluer et par dilution en discontinu
EP0651306A1 (fr) * 1993-10-25 1995-05-03 Alfatech S.R.L. Appareil pour préparer des solutions
US8459129B2 (en) 2008-05-30 2013-06-11 Stéphane Seznec Device for diluting of a sample

Also Published As

Publication number Publication date
DE3231634T1 (de) 1983-12-01
GB2108288B (en) 1985-03-06
GB2108288A (en) 1983-05-11
JPH0442619B2 (fr) 1992-07-14
EP0070893A1 (fr) 1983-02-09
JPS58500337A (ja) 1983-03-03
EP0070893A4 (fr) 1983-06-08

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