UST978003I4 - Reduction of ascorbic acid interference in assays of aqueous fluids - Google Patents

Reduction of ascorbic acid interference in assays of aqueous fluids Download PDF

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Publication number
UST978003I4
UST978003I4 US05/879,573 US87957378A UST978003I4 US T978003 I4 UST978003 I4 US T978003I4 US 87957378 A US87957378 A US 87957378A US T978003 I4 UST978003 I4 US T978003I4
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ascorbic acid
aqueous fluids
assays
reduction
peroxide
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US05/879,573
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Tai-Wing Wu
Shirley S. Yau
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
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  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

interference caused by ascorbic acid is eliminated by the presence of ascorbate oxidase in reagent compositions used in enzymatic assay of aqueous fluids such as blood serum for a specific analyte wherein the analyte is oxidized to peroxide and the peroxide reacts with an indicator to produce a detectable product. A preferred reagent composition contains an enzyme capable of catalyzing in the presence of oxygen the oxidation of the analyte to a peroxide, an indicator composition to produce a detectable product and ascorbate oxidase.
US05/879,573 1977-07-11 1978-02-21 Reduction of ascorbic acid interference in assays of aqueous fluids Pending UST978003I4 (en)

Applications Claiming Priority (2)

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CA282481 1977-07-11
CA282481 1977-07-11

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UST978003I4 true UST978003I4 (en) 1979-01-02

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0014898A1 (en) * 1979-02-27 1980-09-03 C.A. Greiner & Söhne GmbH & Co. KG Test tube for analysis of samples in the clinical field, especially for urine analysis
EP0037056A1 (en) * 1980-03-29 1981-10-07 Roche Diagnostics GmbH Process and diagnostic agents for detecting redox reactions
US4310626A (en) 1980-06-02 1982-01-12 Miles Laboratories, Inc. Interference-resistant composition, device and method for determining a peroxidatively active substance in a test sample

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0014898A1 (en) * 1979-02-27 1980-09-03 C.A. Greiner & Söhne GmbH & Co. KG Test tube for analysis of samples in the clinical field, especially for urine analysis
EP0037056A1 (en) * 1980-03-29 1981-10-07 Roche Diagnostics GmbH Process and diagnostic agents for detecting redox reactions
US4310626A (en) 1980-06-02 1982-01-12 Miles Laboratories, Inc. Interference-resistant composition, device and method for determining a peroxidatively active substance in a test sample

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