USRE42931E1 - Covalent tethering of functional groups to proteins - Google Patents

Covalent tethering of functional groups to proteins Download PDF

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Publication number
USRE42931E1
USRE42931E1 US12/284,010 US28401008A USRE42931E US RE42931 E1 USRE42931 E1 US RE42931E1 US 28401008 A US28401008 A US 28401008A US RE42931 E USRE42931 E US RE42931E
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Prior art keywords
substrate
protein
dhaa
biotin
mutant
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US12/284,010
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Keith V. Wood
Dieter Klaubert
Georgyi V. Los
Robert F. Bulleit
Mark McDougall
Chad Zimprich
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Promega Corp
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Promega Corp
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Assigned to PROMEGA CORPORATION reassignment PROMEGA CORPORATION ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BULLEIT, ROBERT F., LOS, GEORGYI V., WOOD, KEITH V., ZIMPRICH, CHAD, KLAUBERT, DIETER, MCDOUGALL, MARK
Assigned to JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT reassignment JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ORION SEVEN, LLC, PROMEGA AVIATION LLC, PROMEGA BIOSCIENCES, LLC, PROMEGA CORPORATION, TERSO SOLUTIONS, INC.
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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C217/00—Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton
    • C07C217/02—Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton having etherified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C217/04—Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton having etherified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C217/06—Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton having etherified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one etherified hydroxy group and one amino group bound to the carbon skeleton, which is not further substituted
    • C07C217/08—Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton having etherified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one etherified hydroxy group and one amino group bound to the carbon skeleton, which is not further substituted the oxygen atom of the etherified hydroxy group being further bound to an acyclic carbon atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
    • C07C271/06—Esters of carbamic acids
    • C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
    • C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C271/16—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by singly-bound oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/44—Iso-indoles; Hydrogenated iso-indoles
    • C07D209/48—Iso-indoles; Hydrogenated iso-indoles with oxygen atoms in positions 1 and 3, e.g. phthalimide
    • C—CHEMISTRY; METALLURGY
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
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    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
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    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic System
    • C07F5/02—Boron compounds
    • C07F5/022—Boron compounds without C-boron linkages
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y308/00—Hydrolases acting on halide bonds (3.8)
    • C12Y308/01—Hydrolases acting on halide bonds (3.8) in C-halide substances (3.8.1)
    • C12Y308/01005—Haloalkane dehalogenase (3.8.1.5)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

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  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • Immunology (AREA)
  • Biomedical Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biotechnology (AREA)
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  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Pyrane Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
US12/284,010 2003-01-31 2008-09-17 Covalent tethering of functional groups to proteins Expired - Lifetime USRE42931E1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/284,010 USRE42931E1 (en) 2003-01-31 2008-09-17 Covalent tethering of functional groups to proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US44409403P 2003-01-31 2003-01-31
US47465903P 2003-05-30 2003-05-30
US10/768,976 US7238842B2 (en) 2003-01-31 2004-01-30 Covalent tethering of functional groups to proteins
US12/284,010 USRE42931E1 (en) 2003-01-31 2008-09-17 Covalent tethering of functional groups to proteins

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USRE42931E1 true USRE42931E1 (en) 2011-11-15

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Application Number Title Priority Date Filing Date
US10/768,976 Ceased US7238842B2 (en) 2003-01-31 2004-01-30 Covalent tethering of functional groups to proteins
US11/786,792 Expired - Lifetime US7867726B2 (en) 2003-01-31 2007-04-12 Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
US12/284,010 Expired - Lifetime USRE42931E1 (en) 2003-01-31 2008-09-17 Covalent tethering of functional groups to proteins
US12/975,020 Expired - Lifetime US8257939B2 (en) 2003-01-31 2010-12-21 Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
US13/450,217 Expired - Lifetime US8921620B2 (en) 2003-01-31 2012-04-18 Compositions comprising a dehalogenase substrate and a contrast agent and methods of use
US13/450,233 Expired - Lifetime US8895787B2 (en) 2003-01-31 2012-04-18 Compositions comprising a dehalogenase substrate and a radionuclide and methods of use
US14/584,834 Expired - Lifetime US9540402B2 (en) 2003-01-31 2014-12-29 Covalent tethering of functional groups to proteins
US15/376,139 Expired - Lifetime US10240184B2 (en) 2003-01-31 2016-12-12 Covalent tethering of functional groups to proteins
US16/359,581 Expired - Lifetime US11028424B2 (en) 2003-01-31 2019-03-20 Covalent tethering of functional groups to proteins

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US10/768,976 Ceased US7238842B2 (en) 2003-01-31 2004-01-30 Covalent tethering of functional groups to proteins
US11/786,792 Expired - Lifetime US7867726B2 (en) 2003-01-31 2007-04-12 Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use

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US12/975,020 Expired - Lifetime US8257939B2 (en) 2003-01-31 2010-12-21 Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
US13/450,217 Expired - Lifetime US8921620B2 (en) 2003-01-31 2012-04-18 Compositions comprising a dehalogenase substrate and a contrast agent and methods of use
US13/450,233 Expired - Lifetime US8895787B2 (en) 2003-01-31 2012-04-18 Compositions comprising a dehalogenase substrate and a radionuclide and methods of use
US14/584,834 Expired - Lifetime US9540402B2 (en) 2003-01-31 2014-12-29 Covalent tethering of functional groups to proteins
US15/376,139 Expired - Lifetime US10240184B2 (en) 2003-01-31 2016-12-12 Covalent tethering of functional groups to proteins
US16/359,581 Expired - Lifetime US11028424B2 (en) 2003-01-31 2019-03-20 Covalent tethering of functional groups to proteins

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US (9) US7238842B2 (USRE042931-20111115-C00002.png)
EP (5) EP2341134B1 (USRE042931-20111115-C00002.png)
JP (1) JP4748685B2 (USRE042931-20111115-C00002.png)
KR (1) KR20050109934A (USRE042931-20111115-C00002.png)
AU (1) AU2004211584B2 (USRE042931-20111115-C00002.png)
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014093671A1 (en) 2012-12-12 2014-06-19 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US8809529B2 (en) 2010-11-02 2014-08-19 Promega Corporation Imidazo[1,2-α]pyrazine derivatives
US9487520B2 (en) 2010-11-02 2016-11-08 Promega Corporation Coelenterazine derivatives and methods of using same
US9540402B2 (en) 2003-01-31 2017-01-10 Promega Corporation Covalent tethering of functional groups to proteins
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US11913944B2 (en) 2019-03-20 2024-02-27 Promega Corporation Photoaffinity probes

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7429472B2 (en) * 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP3179252B1 (en) * 2004-07-30 2018-11-14 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) * 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
EP1821094A1 (en) * 2004-12-07 2007-08-22 Effector Cell Institute, Inc. Cell measuring method
US20090130130A1 (en) * 2005-06-10 2009-05-21 National University Of Singapore Mutant allergen(s)
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
EP1945262A2 (en) * 2005-10-20 2008-07-23 The Scripps Research Institute Fc labeling for immunostaining and immunotargeting
CA2635747A1 (en) 2006-01-04 2007-07-19 Stanford University Self-illuminating quantum dot systems and methods of use thereof
EP1989548A2 (en) * 2006-02-08 2008-11-12 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
US7449299B2 (en) * 2006-03-10 2008-11-11 David Bauer Quantum dot nanoparticle-based universal neurotoxin biosensor
JP5409354B2 (ja) * 2006-05-25 2014-02-05 インスティチュート フォー アドバンスド スタディ 配列モチーフを同定するための方法、およびその応用
DE102006045607A1 (de) * 2006-09-25 2008-03-27 Leica Microsystems Cms Gmbh Verfahren zur räumlich hochauflösenden Untersuchung von einer mit einer fluoreszierenden Substanz markierten Struktur einer Probe
EP2087107A2 (en) 2006-10-30 2009-08-12 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
FR2934684B1 (fr) 2008-07-31 2012-11-16 Cis Bio Int Methode de detection de l'internalisation de proteines membranaires.
EP2425249B1 (fr) 2009-04-30 2014-07-16 Cisbio Bioassays Procede de detection de composes modulateurs de dimeres de proteines membranaires a domaine vft
FR2949156B1 (fr) 2009-08-13 2016-04-15 Cis-Bio Int Methode de determination de la liaison d'un compose donne a un recepteur membranaire
US8476014B2 (en) * 2010-02-19 2013-07-02 Los Alamos National Security, Llc Probe and method for DNA detection
US9500653B2 (en) 2010-12-07 2016-11-22 Yale University Small-molecule hydrophobic tagging of fusion proteins and induced degradation of same
FR2980271B1 (fr) 2011-09-16 2013-10-11 Cisbio Bioassays Procede de determination de la glycosylation d'un anticorps
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
EP2823270B1 (en) 2012-03-09 2022-05-04 Promega Corporation pH SENSORS
US9267117B2 (en) 2012-03-15 2016-02-23 New England Biolabs, Inc. Mapping cytosine modifications
FR2988174B1 (fr) 2012-03-19 2014-04-25 Cisbio Bioassays Procede de determination de la capacite d'un anticorps a maintenir des cellules a proximite l'une de l'autre
US9585975B2 (en) * 2012-04-27 2017-03-07 Northwestern University MRI contrast agents
KR101923632B1 (ko) 2012-07-31 2018-12-03 이스케이프 테라퓨틱스, 인코퍼레이티드 Hla g-변형된 세포 및 방법
WO2014035712A1 (en) 2012-08-28 2014-03-06 Pierce Biotechnology, Inc. Benzopyrylium compounds
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
EP3312295A1 (en) 2013-03-19 2018-04-25 Directed Genomics, LLC Enrichment of target sequences
WO2015164972A1 (en) * 2014-04-30 2015-11-05 Lanxess Inc. Diluent for the production of butyl rubber
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
US10618907B2 (en) 2015-06-05 2020-04-14 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
EP3430015A1 (en) 2016-03-16 2019-01-23 Oryzon Genomics, S.A. Methods to determine kdm1a target engagement and chemoprobes useful therefor
EP3429575A4 (en) * 2016-03-16 2019-10-23 Purdue Research Foundation AGAINST CARBOANHYDRASE-IX DRUGS AND METHODS
CN113444094B (zh) 2016-07-20 2023-12-22 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
EP3580563A4 (en) 2017-02-09 2020-11-18 Promega Corporation ANALYTE DETECTION IMMUNOLOGICAL ASSAY
US20200278351A1 (en) * 2017-06-23 2020-09-03 Nanotemper Technologies Gmbh Methods for measuring inter- and/or intra-molecular interactions
CN109574880B (zh) 2017-09-29 2022-06-17 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
CN110498799B (zh) 2018-05-18 2022-07-05 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
EP3803401A4 (en) 2018-05-30 2022-02-23 Promega Corporation BROAD-SPECTRUM KINASE BINDERS
US20200200765A1 (en) 2018-12-04 2020-06-25 Promega Corporation Broad spectrum gpcr binding agents
US20220275350A1 (en) 2019-04-16 2022-09-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Circularly permutated haloalkane transferase fusion molecules
EP3816180A1 (en) 2019-10-31 2021-05-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Circularly permutated haloalkane transferase fusion molecules
CN110646417B (zh) * 2019-10-24 2021-10-15 福建医科大学 以纳米金为显色探针的磷酸吡哆醛快速测定方法
WO2021119149A1 (en) 2019-12-10 2021-06-17 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
EP4118233A1 (en) 2020-03-12 2023-01-18 New England Biolabs, Inc. A rapid diagnostic test for lamp
EP4153770A2 (en) 2020-05-22 2023-03-29 Promega Corporation Enhancement of kinase target engagement
WO2022040443A2 (en) 2020-08-21 2022-02-24 New England Biolabs, Inc. A rapid diagnostic test for lamp
WO2022047186A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
KR20230147105A (ko) 2021-01-28 2023-10-20 진콴텀 헬스케어 (쑤저우) 씨오., 엘티디. 리가제 융합 단백질 및 그 응용
US20230203562A1 (en) 2021-12-28 2023-06-29 Encodia, Inc. High-throughput serotyping and antibody profiling assays
WO2023215452A2 (en) 2022-05-04 2023-11-09 Promega Corporation Split modified dehalogenase variants
WO2023215432A1 (en) 2022-05-04 2023-11-09 Promega Corporation Circularly permuted dehalogenase variants
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
WO2023215505A1 (en) 2022-05-04 2023-11-09 Promega Corporation Modified dehalogenase with extended surface loop regions

Citations (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE616245A (fr) 1961-04-14 1962-10-10 Boehringer Sohn Ingelheim Procédé pour la préparation de dérivés de pipéridine
US3131122A (en) 1961-04-14 1964-04-28 Boehringer Sohn Ingelheim Method of producing analgesia with n-substituted-4-phenyl-4-carbalkoxypiperidines
US4574079A (en) 1983-05-27 1986-03-04 Gavras Haralambos P Radiolabeled angiotensin converting enzyme inhibitors for radiolabeling mammalian organ sites
CS259396B1 (en) * 1987-05-06 1988-10-14 Jozef Luston 1,2,2,6,6-pentamethyl-4-(delta-bromalkoxy) piperidines and method of their preparation
US4818807A (en) 1986-09-04 1989-04-04 Idemitsu Kosan Co., Ltd. Liquid-crystalline polymer
US5071469A (en) 1989-04-21 1991-12-10 E. I. Du Pont De Nemours And Company Herbicidal benzylsulfonamides
US5099020A (en) 1989-11-27 1992-03-24 Abbott Laboratories Barbiturate assay compositions and methods
US5110833A (en) 1989-01-16 1992-05-05 Klaus Mosbach Preparation of synthetic enzymes and synthetic antibodies and use of the thus prepared enzymes and antibodies
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5372944A (en) 1993-10-14 1994-12-13 The Dow Chemical Company Method for conversion of halogenated hydrocarbons to halohydrins
US5476770A (en) 1993-01-28 1995-12-19 Commissariat A L'energie Atomique Immunometric determination of an antigen or hapten
US5503977A (en) 1994-04-22 1996-04-02 California Institute Of Technology Split ubiquitin protein sensor
US5523209A (en) 1994-03-14 1996-06-04 The Scripps Research Institute Methods for identifying inhibitors of integrin activation
EP0718300A1 (en) 1994-12-22 1996-06-26 Nisshinbo Industries, Inc. Carbodiimide derivative
US5576424A (en) 1991-08-23 1996-11-19 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
US5700908A (en) 1989-05-25 1997-12-23 La Jolla Cancer Research Foundation β3 integrin cytoplasmic domain specific peptide and nucleic acid
US5786428A (en) 1996-03-27 1998-07-28 California Institute Of Technology Adsorbents for amino acid and peptide separation
WO1998036080A1 (en) 1997-02-13 1998-08-20 The Dow Chemical Company Recombinant haloaliphatic dehalogenases
US5821047A (en) 1990-12-03 1998-10-13 Genentech, Inc. Monovalent phage display
US5932421A (en) 1996-12-06 1999-08-03 The Scripps Research Institute Methods and cell lines for identification of regulators of integrin activation
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6255461B1 (en) 1996-04-05 2001-07-03 Klaus Mosbach Artificial antibodies to corticosteroids prepared by molecular imprinting
WO2001053303A1 (fr) * 2000-01-20 2001-07-26 Centre National De La Recherche Scientifique (Cnrs) Composes organosilicies, leur procede de preparation et leurs utilisations
WO2001060415A1 (en) 2000-02-18 2001-08-23 The Immune Response Corporation Methods and compositions for gene delivery
WO2001077668A2 (en) 2000-04-10 2001-10-18 The Scripps Research Institute Proteomic analysis using active-site directed probes
US6333154B1 (en) 1997-12-04 2001-12-25 Institut Pasteur Bacterial multi-hybrid system and applications thereof
WO2002028841A2 (en) 2000-10-02 2002-04-11 Molecular Probes, Inc. Reagents for labeling biomolecules having aldehyde or ketone moieties
US20020042055A1 (en) 1999-12-23 2002-04-11 Affholter Joseph A. Alteration of hydrolase genes and screening of the resulting libraries for the ability to catalyze specific reactions
US6416733B1 (en) 1996-10-07 2002-07-09 Bristol-Myers Squibb Pharma Company Radiopharmaceuticals for imaging infection and inflammation
WO2002057411A2 (en) 2000-12-01 2002-07-25 Diversa Corporation Hydrolase enzymes and their use in kinetic resolution
WO2002068583A2 (en) 2000-12-01 2002-09-06 Diversa Corporation Enzymes having dehalogenase activity and methods of use thereof
WO2002083937A2 (en) 2001-04-10 2002-10-24 Ecole Polytechnique Federale De Lausanne Methods using o6-alkylguanine-dna alkyltransferases
US6492560B2 (en) 1995-10-19 2002-12-10 The University Of Washington Discrete-length polyethylene glycols
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
WO2003040096A2 (en) 2001-11-08 2003-05-15 Elan Pharmaceuticals, Inc. N, n'-substituted-1,3-diamino-2-hydroxypropane derivatives
WO2004009788A2 (en) 2002-07-19 2004-01-29 Diversa Corporation Fluorescent proteins, nucleic acids encoding them and methods for making and using them
US20040152880A1 (en) 2002-11-22 2004-08-05 Carnegie Mellon University Compositions and methods for the reversible capture of biomolecules
WO2004072232A2 (en) 2003-01-31 2004-08-26 Promega Corporation Covalent tethering of functional groups to proteins
US6800453B2 (en) 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
US20050095651A1 (en) 2003-08-12 2005-05-05 The Regents Of The University Of California Photoswitchable method for the ordered attachment of proteins to surfaces
US20050272114A1 (en) 2003-01-31 2005-12-08 Aldis Darzins Substrates for covalent tethering to proteins
US20060024808A1 (en) 2004-07-30 2006-02-02 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
WO2007092579A2 (en) 2006-02-08 2007-08-16 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
WO2008054821A2 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4612302A (en) 1983-11-14 1986-09-16 Brigham And Women's Hospital Clinical use of somatostatin analogues
US4684620A (en) 1984-09-04 1987-08-04 Gibson-Stephens Neuropharmaceuticals, Inc. Cyclic polypeptides having mu-receptor specificity
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
DE3614647A1 (de) * 1986-04-30 1987-11-05 Euratom 7-phenylessigsaeure-4-alkyl-coumarinylamide, verfahren zu ihrer herstellung sowie ihre verwendung in verfahren zur fluorometrischen bestimmung der aktivitaet von hydrolasen, insbesondere von penicillin-g-acylase
US4853371A (en) 1986-06-17 1989-08-01 The Administrators Of The Tulane Educational Fund Therapeutic somatostatin analogs
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
ATE180018T1 (de) * 1991-08-23 1999-05-15 Molecular Probes Inc Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen
CH689633A5 (de) 1995-01-10 1999-07-30 Von Roll Umwelttechnik Ag Verfahren zur Kuehlung und Reinigung von Rauchgasen.
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
GB0111987D0 (en) * 2001-05-16 2001-07-04 Univ Sheffield Method
US7195882B2 (en) * 2003-06-03 2007-03-27 Roche Diagnostics Operations, Inc. Monoclonal antibodies specific for buprenorphine and metabolites thereof

Patent Citations (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3131122A (en) 1961-04-14 1964-04-28 Boehringer Sohn Ingelheim Method of producing analgesia with n-substituted-4-phenyl-4-carbalkoxypiperidines
BE616245A (fr) 1961-04-14 1962-10-10 Boehringer Sohn Ingelheim Procédé pour la préparation de dérivés de pipéridine
US4574079A (en) 1983-05-27 1986-03-04 Gavras Haralambos P Radiolabeled angiotensin converting enzyme inhibitors for radiolabeling mammalian organ sites
US4818807A (en) 1986-09-04 1989-04-04 Idemitsu Kosan Co., Ltd. Liquid-crystalline polymer
CS259396B1 (en) * 1987-05-06 1988-10-14 Jozef Luston 1,2,2,6,6-pentamethyl-4-(delta-bromalkoxy) piperidines and method of their preparation
US5110833A (en) 1989-01-16 1992-05-05 Klaus Mosbach Preparation of synthetic enzymes and synthetic antibodies and use of the thus prepared enzymes and antibodies
US5071469A (en) 1989-04-21 1991-12-10 E. I. Du Pont De Nemours And Company Herbicidal benzylsulfonamides
US5700908A (en) 1989-05-25 1997-12-23 La Jolla Cancer Research Foundation β3 integrin cytoplasmic domain specific peptide and nucleic acid
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5099020A (en) 1989-11-27 1992-03-24 Abbott Laboratories Barbiturate assay compositions and methods
US5821047A (en) 1990-12-03 1998-10-13 Genentech, Inc. Monovalent phage display
US5576424A (en) 1991-08-23 1996-11-19 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
US5476770A (en) 1993-01-28 1995-12-19 Commissariat A L'energie Atomique Immunometric determination of an antigen or hapten
US5372944A (en) 1993-10-14 1994-12-13 The Dow Chemical Company Method for conversion of halogenated hydrocarbons to halohydrins
US5523209A (en) 1994-03-14 1996-06-04 The Scripps Research Institute Methods for identifying inhibitors of integrin activation
US5503977A (en) 1994-04-22 1996-04-02 California Institute Of Technology Split ubiquitin protein sensor
EP0718300A1 (en) 1994-12-22 1996-06-26 Nisshinbo Industries, Inc. Carbodiimide derivative
US5700935A (en) 1994-12-22 1997-12-23 Nisshinbo Industries, Inc. Carbodiimide derivative
US6492560B2 (en) 1995-10-19 2002-12-10 The University Of Washington Discrete-length polyethylene glycols
US5786428A (en) 1996-03-27 1998-07-28 California Institute Of Technology Adsorbents for amino acid and peptide separation
US6255461B1 (en) 1996-04-05 2001-07-03 Klaus Mosbach Artificial antibodies to corticosteroids prepared by molecular imprinting
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6416733B1 (en) 1996-10-07 2002-07-09 Bristol-Myers Squibb Pharma Company Radiopharmaceuticals for imaging infection and inflammation
US5932421A (en) 1996-12-06 1999-08-03 The Scripps Research Institute Methods and cell lines for identification of regulators of integrin activation
WO1998036080A1 (en) 1997-02-13 1998-08-20 The Dow Chemical Company Recombinant haloaliphatic dehalogenases
US6333154B1 (en) 1997-12-04 2001-12-25 Institut Pasteur Bacterial multi-hybrid system and applications thereof
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US20020042055A1 (en) 1999-12-23 2002-04-11 Affholter Joseph A. Alteration of hydrolase genes and screening of the resulting libraries for the ability to catalyze specific reactions
WO2001053303A1 (fr) * 2000-01-20 2001-07-26 Centre National De La Recherche Scientifique (Cnrs) Composes organosilicies, leur procede de preparation et leurs utilisations
US20030166957A1 (en) 2000-01-20 2003-09-04 Bernard Benneteau Organosilicon compounds, preparation method and uses thereof
WO2001060415A1 (en) 2000-02-18 2001-08-23 The Immune Response Corporation Methods and compositions for gene delivery
WO2001077668A2 (en) 2000-04-10 2001-10-18 The Scripps Research Institute Proteomic analysis using active-site directed probes
WO2002028841A2 (en) 2000-10-02 2002-04-11 Molecular Probes, Inc. Reagents for labeling biomolecules having aldehyde or ketone moieties
WO2002057411A2 (en) 2000-12-01 2002-07-25 Diversa Corporation Hydrolase enzymes and their use in kinetic resolution
US20020137171A1 (en) 2000-12-01 2002-09-26 Diversa Corporation Hydrolase enzymes and their use in kinetic resolution
WO2002068583A2 (en) 2000-12-01 2002-09-06 Diversa Corporation Enzymes having dehalogenase activity and methods of use thereof
US7078504B2 (en) 2000-12-01 2006-07-18 Diversa Corporation Enzymes having dehalogenase activity and methods of use thereof
US6800453B2 (en) 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
US20050048580A1 (en) 2001-01-23 2005-03-03 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
WO2002083937A2 (en) 2001-04-10 2002-10-24 Ecole Polytechnique Federale De Lausanne Methods using o6-alkylguanine-dna alkyltransferases
WO2003040096A2 (en) 2001-11-08 2003-05-15 Elan Pharmaceuticals, Inc. N, n'-substituted-1,3-diamino-2-hydroxypropane derivatives
WO2004009788A2 (en) 2002-07-19 2004-01-29 Diversa Corporation Fluorescent proteins, nucleic acids encoding them and methods for making and using them
US20040152880A1 (en) 2002-11-22 2004-08-05 Carnegie Mellon University Compositions and methods for the reversible capture of biomolecules
US20040214258A1 (en) 2003-01-31 2004-10-28 Wood Keith V Covalent tethering of functional groups to proteins
WO2004072232A2 (en) 2003-01-31 2004-08-26 Promega Corporation Covalent tethering of functional groups to proteins
US20050272114A1 (en) 2003-01-31 2005-12-08 Aldis Darzins Substrates for covalent tethering to proteins
US20080026407A1 (en) 2003-01-31 2008-01-31 Promega Corporation Covalent tethering of functional groups to proteins
US20090098627A1 (en) 2003-01-31 2009-04-16 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US7238842B2 (en) 2003-01-31 2007-07-03 Promega Corporation Covalent tethering of functional groups to proteins
US20050095651A1 (en) 2003-08-12 2005-05-05 The Regents Of The University Of California Photoswitchable method for the ordered attachment of proteins to surfaces
US20060024808A1 (en) 2004-07-30 2006-02-02 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US20080274488A1 (en) 2004-07-30 2008-11-06 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
WO2006093529A2 (en) 2004-07-30 2006-09-08 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070224620A1 (en) 2006-02-08 2007-09-27 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
WO2007092579A2 (en) 2006-02-08 2007-08-16 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
WO2008054821A2 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
US20080145882A1 (en) 2006-10-30 2008-06-19 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression

Non-Patent Citations (181)

* Cited by examiner, † Cited by third party
Title
"Australian Application Serial No. 2004211584, First Examiner Report mailed Jan. 6, 2009", 3 pgs.
"Australian Application Serial No. 2004211584, Response filed Sep. 9, 2009 to First Examiner Report mailed Jan. 6, 2009", 28 pgs.
"Chinese Application Serial No. 200480008194.4, Response filed to Second Office Action mailed May 22, 2009", (2009), 14 pgs.
"Chinese Application Serial No. 200480008194.4, Second Office Action mailed May 22, 2009", 15 pgs.
"Chinese Patent Application No. 20048000819.4, First Office Action mailed Dec. 22, 2006", 8 pgs.
"Chinese Patent Application No. 200480008194, Response filed Jul. 6, 2007 to the First Action mailed mailed 12-22-22-2006", 27 pgs.
"European Application Serial No. 04707032.1, Communication mailed Jun. 22, 2009", 6 pgs.
"European Patent Application Ser. No. 0407032.1, Response filed Sep. 18, 2007 to Examining Division's Communication mailed Mar. 12, 2007", 37 pgs.
"European Patent Application Ser. No. 04707032.1, Communication Pursuant to Article 96(2) EPC mailed Mar. 12, 2007", 3 pgs.
"Functional Group", Encyclopaedia Britannica Article Online, http://www.search.ed.com/eb/article-9035655, (Mar. 19, 2007).
"Indian Application Serial No. 3867/DELNP/2005, First Examination Report dated Sep. 28, 2007", 19 pgs.
"Indian Application Serial No. 3867/DELNP/2005, Response filed Mar. 19, 2008 to First Examination Report dated Sep. 28, 2007", 21 pgs.
"Indian Application Serial No. 3867/DELNP/2005, Response filed Sep. 23, 2008 to Examination Report Sep. 9, 2008", 5 pgs.
"Partial Search Report for corresponding PCT Application No. PCT/US2005/027307", (Jun. 11, 2006), 5 pgs.
"PCT Application No. PCT/US2004/002607, International Preliminary Report on Patentability mailed Jun. 23, 2005", 16 pgs.
"PCT Application No. PCT/US2004/002607, International Search Report mailed Oct. 26, 2004", 4 pgs.
"Prosecution File History for U.S. Appl. No. 10/768,976", (issued as US 7,238,842), 141 pgs., Jul. 2007.
"U.S. Appl. No. 10/768,976 Final Office Action mailed Mar. 14, 2006", 9 pgs.
"U.S. Appl. No. 10/768,976 Non Final Office Action mailed Aug. 1, 2006", 7 pgs.
"U.S. Appl. No. 10/768,976 Non Final Office Action mailed Sep. 9, 2005", 12 pgs.
"U.S. Appl. No. 10/768,976 Notice of Allowance mailed Dec. 15, 2006", 11 pgs.
"U.S. Appl. No. 10/768,976 Preliminary Amendment and Response to Restriction Requirement filed Aug. 5, 2005", 22 pgs.
"U.S. Appl. No. 10/768,976 Response filed Jan. 24, 2006 to Non Final Office Action mailed Sep. 9, 2005", 21 pgs.
"U.S. Appl. No. 10/768,976 Response filed Jul. 11, 2006 to Final Office Action mailed Mar. 14, 2006", 13 pgs.
"U.S. Appl. No. 10/768,976 Response filed Nov. 1, 2006 to Non Final Office Action mailed Aug. 1, 2006", 13 pgs.
"U.S. Appl. No. 10/768,976, Restriction Requirement mailed Jun. 2, 2005", 11 pgs.
"U.S. Appl. No. 11/006,031 Advisory Action mailed May 7, 2007", 3 pgs.
"U.S. Appl. No. 11/006,031 Amendment and Response filed Apr. 25, 2007 to Final Office Action mailed Feb. 8, 2007", 34 pgs.
"U.S. Appl. No. 11/006,031 Final Office Action mailed Feb. 8, 2007", 8 pgs.
"U.S. Appl. No. 11/006,031 Non Final Office Action mailed Aug. 9, 2006", 13 pgs.
"U.S. Appl. No. 11/006,031 Non Final Office Action mailed Mar. 15, 2006", 15 pgs.
"U.S. Appl. No. 11/006,031 Notice of Allowance mailed Apr. 24, 2008", 7 pgs.
"U.S. Appl. No. 11/006,031 Notice of Allowance Mailed Oct. 30, 2007", 8 pgs.
"U.S. Appl. No. 11/006,031 Response filed Dec. 22, 2006 to Non Final Office Action mailed Aug. 9, 2006", 37 pgs.
"U.S. Appl. No. 11/006,031 Response filed Feb. 17, 2006 to Restriction Requirement mailed Dec. 21, 2005", 34 pgs.
"U.S. Appl. No. 11/006,031 Response filed Jul. 6, 2006 to Non Final Office Action mailed Mar. 15, 2006", 35 pgs.
"U.S. Appl. No. 11/006,031 Response filed Jul. 7, 2007 to Advisory Action mailed May 7, 2007", 32 pgs.
"U.S. Appl. No. 11/006,031, Response to Restriction Requirement filed Aug. 31, 2007", 37 pgs.
"U.S. Appl. No. 11/006,031, Restriction Requirement mailed Dec. 21, 2005", 20 pgs.
"U.S. Appl. No. 11/006,031, Restriction Requirment mailed Jul. 31, 2007", 8 pgs.
"U.S. Appl. No. 11/194,110 Non Final Office Action mailed Mar. 29, 2007", 21 pgs.
"U.S. Appl. No. 11/194,110 Notice of Allowance mailed Aug. 15, 2007", NOAR, 12 pgs.
"U.S. Appl. No. 11/194,110 Preliminary Amendment mailed Oct. 31, 2007", 18 pgs.
"U.S. Appl. No. 11/194,110 Response filed Jun. 29, 2007 to Non Final Office Action mailed Mar. 29, 2007", 20 pgs.
"U.S. Appl. No. 11/194,110, Notice of Allowance Mailed Dec. 3, 2007", 6 pgs.
"U.S. Appl. No. 11/194,110, Response to Restriction Requirement and Preliminary Amendment mailed Feb. 7, 2007", 21 pgs.
"U.S. Appl. No. 11/194,110, Restriction Requirement mailed Jan. 4, 2007", 14 pgs.
"U.S. Appl. No. 11/194,110, Supplemental Preliminary Amendment mailed Nov. 14, 2007", 6 pgs.
"U.S. Appl. No. 11/704,150 Restriction Requirement mailed Mar. 30, 2009", 11 pgs.
"U.S. Appl. No. 11/704,150, Non-Final Office Action mailed Sep. 8, 2009", 12 pgs.
"U.S. Appl. No. 11/704,150, Response filed May 6, 2009 to Restriction Requirement mailed Mar. 30, 2009", 12 pgs.
"U.S. Appl. No. 11/709,150 Preliminary Amendment filed May 11, 2007", 12 pgs.
"U.S. Appl. No. 11/786,792 Preliminary Amendment filed Apr. 12, 2007", 11 pgs.
"U.S. Appl. No. 11/786,792 Preliminary Amendment filed Aug. 17, 2007", 13 pgs.
"U.S. Appl. No. 11/786,792, Final Office Action mailed Jul. 10, 2009", 14 pgs.
"U.S. Appl. No. 11/786,792, Non-Final Office Action mailed Feb. 5, 2009", 10 pgs.
"U.S. Appl. No. 11/786,792, Response filed Dec. 23, 2008 to Restriction Requirement mailed Nov. 26, 2008", 15 pgs.
"U.S. Appl. No. 11/786,792, Response filed Jun. 2, 2009 to Non-Final Office Action mailed Feb. 5, 2009", 16 pgs.
"U.S. Appl. No. 11/786,792, Response filed Sep. 28, 2009 to Final Office Action mailed Jul. 10, 2009", 17 pgs.
"U.S. Appl. No. 12/075,160, Restriction Requirement mailed Sep. 23, 2009", 8 Pgs.
"U.S. Appl. No. 12/220,478, Preliminary Amendment filed Dec. 2, 2008", 3 pgs.
"Valence," Hawley's Condensed Chemical Dictionary (14th Edition) Online, John Wiley and Sons, 2002, http://www.knovel.com/knove12/Toc.jsp?BookID=704&VerticlID=0 (Mar. 20, 2007).
"International Search Report from corresponding PCT Application No. PCT-US2004-002607", (Oct. 26, 2004), 4 Pages.
Adachi, H., et al., "Site-directed mutants, at position 166, of RTEM-1 beta-lactamase that form a stable acyl-enzyme intermediate with penicillin", J. Biol Chem., 266(5), (Feb. 1991), pp. 3186-3191.
Adamczyk, M. et al., "Surface plasmon resonance (SPR) as a tool for antibody conjugate analysis," Bioconjug. Chem. (1999) 10(6):1032-1037.
Affholter, J. A., et al., "Recombinant Haloaliphatic Dehalogenases", EMBL Database, Genetic Sequence, Entry Name: EMBL:BD057138, (Sep. 4, 2002), 1 p.
Akiyama, M., et al., "N-Hydroxy Amides, Part 8. Synthesis and Fe(III) Holding Properties of di- and Trihydroxamic Acids Extending from Benezenedi- and Tricarbonyl Units Through Oligo(ethyleneoxy) Arms", J. Chem. Soc. Perkin Transactions, 2 Physical Org Chem, 9, (1989), 1213-1218.
Albertson, Noel, et al., "A Synthesis of DL-Proline", Journal of the American Chemical Society,71, (1949), 2818-2820.
Amat-Guerri, F. et al., "Methacrylate-tethered analogs o the laser dye PM567 synthesis, copolymerization with methyl methacrylate and photostabilit of the copolymers," Phytochem. Photobiol. (2003) 77(6):577-584.
Arand, M., et al., "Sequence similarity of mammalian epoxide hydrolases to the bacterial haloalkanedehalogenase and other related proteins", FEBS Letters, 338, 1994 , 251-256.
Aravind, L., "An evolutionary classification of the metallo-beta-lactamase fold proteins", In Silico Biol., 1(2), (1999), pp. 69-91.
Banas, P. et al., "Mechanism of enhanced conversion of 1,2,3-trichloropropane by mutant haloalkane dehalogenase revealed by molecular modeling," J. Computer-Aided Molecular Design (2006) 20(6):375-383.
Banks, P., et al., "Understanding Fluorescene Polarization and its Data Analysis—Physical Principles of Fluorescence Polarization", http://www.perkinelmer.com/lifesciences, (2001), 12 Pages.
Barrett, A. G.M, "Synthesis and Characterization of a New Polymer Support for a Metallocene Catalyst", Tetrahedron, 58 (19), Elsevier Science Publishers, (May 6, 2002), 3785-3792.
Bier, C , "Covalys—one tag does it all", Bio World, (2003), pp. 46-47.
Bier, C, "Covalys—one tag does it all", Market Portrait, (2003), pp. 46-47.
Bodwell, G. J, et al., "Synthesis, Structure and AM1 Conformational Study of [3] Paracyclo[3] (1,3) indolophane, a Novel Chiral Cyclophane", Tetrahedron, 55(45), (Nov. 5, 1999), 12939-12956.
Bosma, et al., "Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene", Appl Environ Microbiol, (Jul. 2002), 3582-7.
Campbell, R. E., et al., "A Monomeric Red Fluorescent Protein", PNAS, 99(12), (2002), 7877-7882.
Castro, C. E, et al., "Biodehalogenation, Reductive Reactivities of Microbial and Mammalian Cytochromes P-450 Compared with Heme and Whole-cell Models", Journal of Agricultural & Food Chemistry, 36, (1988), 915-919.
Chaloupova, R, "Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel", J Biol Chem., (Dec. 26, 2003), 52622-8.
Chen, C. , "Relocation of the catalytic carboxylate group in class A beta-lactamase: the structure and function of the mutant enzyme Glu-166->Gln:Asn170->Asp.", Protein Eng., 12(7), (Jul. 1999), pp. 573-579.
Chen, C. H, et al., "Relocation of the Catalytic Carboxylate Group in Class A Beta-lactamase: The Structure and Function of the Mutant Enzyme GIu166-Gln: Asn170", Protein Engineering, Oxford University Press, Surrey GB, 12(7), (Jul. 1999) , 573-579.
Chen, I., et al., "Site-specific labeling of proteins with small molecules in live cells", Current Opinion in Biotechnology, 16, (2005), 35-40.
Cheuk et al., "Synthesis of optically active poly(phenylacetylenes) containing amino acid pendent groups," Polymeric Materials Science and Engineering 82:56-57, 2000. *
Cheuk, K. L.C, "Synthesis of Optically Active Poly(Phenylacetylenes) Containing Amino Acid Pendent Groups", Polymeric Materials Science and Engineering, 82, (2000), 56-57.
Chinese Patent Office Action for Application No. 200480008194.4 dated Oct. 9, 2009 (21 pages) with English translation.
Cohen, Victor, et al., "Synthesis of some Substituted Dibenzodiazepinones and Pyridobenzodiazepinones", Journal of Heterocyclic Chemistry, 35, (1998), 675-686.
Curragh et al., "Haloalkane degradation and assimilation by Rhodococcus rhodocrous NCIMB 13064," Microbiology 140:1433-1442, 1994. *
Dahl, K. H, et al., "The Reactivity of Affinity Labels: A Kinetic Study of the Reaction of Alkyl Halides with Thiolate Anions—a Model Reaction for Protein Alkylation", Bioorganic Chemistry,10, (1981), 329-341.
Database EMBL EBI, Accession No. BD057138, (2004), 1 pg.
Database EMBL EBI, Accession No. BD057138, 1 pg.
Dorwald, F.A., "Side reactions in organic synthesis," A Guide of Successful Synthesis Design, Wiley: VCH, Weinheim (2005) 4 pages.
Doubrovin, M., et al., "Reviews—Multimodality in Vivo Molecular-Genetic Imaging", Bioconjugate Chem., 15, (2004), 1376-1388.
European Patent Office Action for Application No. 05857556.4 dated May 7, 2009 (3 pages).
European Patent Office Action for Application No. 07763411.1 dated Dec. 17, 2008 (2 pages).
European Patent Office Action for Application No. 07763411.1 dated Mar. 17, 2009 (5 pages).
European Patent Office Action for Application No. 07867352.2 dated Feb. 1, 2010 (4 pages).
Farinas, J., "Receptor-mediated Targeting of Fluorescent Probes in Living Cells", The Journal of Biological Chemistry, 274(12), (1999), 7603-7606.
Franken, S., et al., "Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes", EMBO J., 10(6), (Jun. 1991), pp. 1297-1302.
Gambhir, S. S., "Molecular Imaging of Cancer With Positron Emission Tomography", Nature Reviews, 2, 2002 , 683-693.
Gao, X. et al., "Construction of murine phage antibody library and selection of ricin-specific single-chain antibodies," IUBMB Life (1999) 48(5):513-517.
Gibbons, F. D., et al., "Chipper: Discovering Transcription-Factor Targets From Chromatin Immunoprecipitation Microarrays Using Variance Stabilization", Genome Biology, 6(11): Article R96, (2005), 9 pgs.
Gite, S., et al., "Ultrasensistive Fluorescence-Based Detection of Nascent Proteins in Gels", Analytical Biochemistry, 279, (2000), 218-225.
Gould, K. L., et al., "Tandem Affinity Purification and Identification of Protein Complex Components", Methods, 33, (2004), 239-244.
Gray, K, et al., "Rapid evolution of reversible denaturation and elevated melting temperature in a microbial haloalkane dehalogenase", Adv. Synth. Catal., (2001), 607-17.
Griffin, B. A., et al., "Specific covalent labeling of recombinant protein molecules inside live cells", Science, 281(5374), (Jul. 1998), 269-72.
Gurskaya, N. G., et al., "GFP-like Chromoproteins as a Source of Far-red Fluorescent Proteins", FEBS Letters, 507, (2001), 16-20.
Hall, D. A., et al., "Regulation of Gene Expression by a Metabolic Enzyme", Science, 306, (2004), 482-484.
Heck, R. F, "Aromatic Haloethylation with Palladium and copper Halides", Journal of the American Chemical Society, 90, (1968), 5538-5542.
Henze, H.R. et al., "The number of structurally isomeric alcohols of the methanol series," J. Amer. Chem. Soc. (1931) 53:3042-3046.
Hodneland, C. D., et al., "Selective Immobilization of Proteins to Self-Assembled Monolayers Presenting Active Site-Directed Capture Ligands", Proc. Natl. Acad. Sci. USA, 99(8), (Apr. 16, 2002), 5048-5052.
Holloway, P., et al., "A Colorimetric Assay for Detecting Haloalkine Dehalogenase Activity", Journal of Microbiological Materials, 32, (1998), 31-36.
Horton, R. H, et al., "Reactions with Reactive Alkyl Halidies", Methods in Enzymology, 11, (1967), 556-565.
Huber, W., et al., "SPR-based Interaction Studies With Small Molecular Weight Ligands Using hAGT Fusion Proteins", Analytical Biochemistry, 333, (2004), 280-288.
Hynkova, K., et al., "Identification of the Catalytic Triad in the Haloalkane Dehalogenase from Sphingomonas paucimobilis UT26", FEBS Letters, 446 (1), (Mar. 5, 1999), 177-181.
Ichiyama, S., et al., "Novel Catalytic Mechanism of Nucleophilic Substitution by Asparagine Residue Involving Cyanoalanine Intermediate Revealed by Mass Spectrometric Monitoring of an Enzyme", Journal of Biological Chemistry, 275 (52), (Dec. 29, 2000), 40804-40809.
Indian Patent Office Action for Application No. 3867/DELNP/2005 dated Sep. 9, 2008 (1 page).
Instant Notes-Chemistry for Biologists, Second Edition, Fisher & Arnolds, Garland Science/BIOS Scientific Publishers (2004) 245-246.
International Search Report and Written Opinion for Application No. PCT/US2005/027307 dated Jan. 29, 2007 (27 pages).
International Search Report and Written Opinion for Application No. PCT/US2007/003416 dated Sep. 14, 2007 (17 pages).
International Search Report and Written Opinion for Application No. PCT/US2007/023205 dated Nov. 3, 2008 (12 pages).
Japanese Patent Office Action for Application No. 2006-503174 mailed Oct. 27, 2009 (18 pages) with English translation.
Jeong, S. et al., "Kinase assay based on thiophosphorylation and biotinylation," Biotechniques (1999) 27(6):1232-1238.
Jones, T. C, et al., "Solvolysis Mechanisms, SNI-Like Behavior of Methyl Chloromethyl Ether, Sensitivity to Solvent Ionizing Power and alpha-Deuterium Isotope Effect", Journal of the American Chemical Society,89, (1967), 4863-4867.
Keppler, A., et al., "A general method for the covalent labeling of fusion proteins with small molecules in vivo", Nature Biotechnology, 21(1), (Jan. 20, 2003), 86-89.
Krooshof, G., et al., "Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure", Biochemistry, 36(31), (Aug. 1997), 9571-80.
Kulakova, A., et al., "The Plasmid-Located Haloalkane Dehalogenase Gene from Rhodococcus rhodochrous NCIMB 13064", Microbiology, 143, (Jan. 1997), 109-115.
Kurihara, T., et al., "Comprehensive Site-directed Mutagenesis of L-2-Halo Acid Dehalogenase to Probe Catalytic Amino Acid Residues", Journal of Biochemistry, 117(6), (Mar. 15, 1995), 1317-1322.
Kwon, Y, et al., "Antibody Arrays Prepared by Cutinase-Mediated Immobilization on Self-Assembled Monolayers", Analytical Chemistry,76(19), (Oct. 1, 2004), 5713-5720.
Lautens, M., et al., "An Expedient Route for the Stereoselective Construction of Bridged Polyheterocyclic Ring Systems Using the Tandem "Pincer" Diels-Alder Reaction", J. Org. Chem., 62, (1997), 4418-4427.
Lewis, B. et al., "Detection and quantification of biotinylated proteins using the storm 840 optical scanner," J. Nutri. Biochem. (2003) 14:196-202.
Li, Q., et al., "A Modified Mammalian Tandem Affinity Purification Procedure to Prepare Functional Polycystin-2 Channel", FEBS Letters, 576, (2004), 231-236.
Lin, Z., et al., "Methods for Labeling Quantum Dots to Biomolecules", Journal of Nanoscience and Nanotechnology, 4(6), (2004), 641-645.
Los, G. V, et al., "Chapter 14—The Halo Tagtm—A Novel Technology for Cell Imaging and Protein Analysis", Methods in Molecular Biology,356, (2007), 195-208.
Luo, X.-L., et al., "A Glucose Biosensor Based on Chitsosan-Glucose Oxidase-Gold Nanoparticles Biocomposite Formed by One-Step Electrodeposition", Analytical Biochemistry, 334, (2004), 284-289.
Manoury, P. M, et al., "Synthesis of a Series of Compounds Related to Betaxolol, a New B1-Adrenoceptor Antagonist with a Pharmacological and Pharmacokinetic Profile Optimized for the Treatment of Chronic Cardiovascular Diseases", J. Med. Chem., 30(6), (1987), 1003-1011.
Mathieu, S., et al., "Monitoring E-Selection-Mediated Adhesion Using Green and Red Fluorescent Proteins", Journal of Immunological Methods, 272, (2003), 81-92.
Michl, J., et al., "", Electronic Aspects of Organic Photochemistry, (John Wiley & Sons, 1990), 61-78.
Michl, J., et al., Electronic Aspects of Organic Photochemistry, (John Wiley & Sons, 1990),61-78.
Miller, L. W, et al., "Selective Chemical Labeling of proteins in Living Cells", Current opinion in chemical Biology, 9, (2005), 56-61.
Momose, Y., et al., "Novel 5-Substituted-1H-Tetrazole Derivatives as Potent Glucose and Lipid Lowering Agents", Chemical and Pharmaceutical Bulletin, 50(1), (Jan. 2002), 100-111.
Morzycki et al., "Synthesis of dimeric steroids as components of lipid membranes," Tetrahedron 53(30):10579-10590, 1997. *
Morzycki, J. W, et al., "Synthesis of Dimeric Steroids as Components of Lipid Membranes", Tetrahedron, 53(30), (1997), 10579-10590.
Newman, J., et al., "Haloalkane Dehalogenases: Structure of a Rhodococcus Enzyme", Biochemistry, 38(49), 1999 , 16105-16114.
Office Action for U.S. Appl. No. 11/006,031 dated May 7, 2007 (3 pages).
Office Action for U.S. Appl. No. 11/509,796 dated Apr. 11, 2007 (32 pages).
Office Action for U.S. Appl. No. 11/509,796 dated Mar. 28, 2008 (12 pages).
Office Action for U.S. Appl. No. 11/509,796 dated Nov. 12, 2008 (22 pages).
Office Action for U.S. Appl. No. 11/509,796 dated Sep. 24, 2007 (34 pages).
Office Action for U.S. Appl. No. 11/786,792 dated Jun. 7, 2010 (7 pages).
Office Action for U.S. Appl. No. 11/786,792 dated Nov. 2, 2009 (12 pages).
Office Action for U.S. Appl. No. 12/075,160 dated May 24, 2010 (6 pages).
Office Action for U.S. Appl. No. 12/075,160 dated Nov. 16, 2009 (6 pages).
Office Action for U.S. Appl. No. 12/220,478 dated Jun. 10, 2010 (11 pages).
Pieters, R., et al., "Design and synthesis of reagents for phage display screening of dehalogenases", Bioorganic & Medicinal Chemistry Letters, 9(2), (Jan. 1999), pp. 161-166.
Pries, F., et al., "Activation of an Asp-124-> Asn Mutant of Haloalkane Dehalogenase by Hydrolytic Deamidation of Asparagine", FEBS Letters, 358 (2), (1995), 171-174.
Pries, F., et al., "Histidine 289 is essential for hydrolysis of the alkyl-enzyme intermediate of haloalkane dehalogenase", Journal of Biological Chemistry, 270(18), (May 1995), 10405-10411.
Pulig, O., et al., "The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification", Methods, 24, (2001), 218-229.
Result 1, alignment of Applicants' dehalogenase sequence (instant SEQ ID No. 82 having C and G substituted at positions 106 and 176, respectively) with SEQ ID No. 40 of Short et al., US 7,078,504, search of issued patents database, May 19, 2010 (2 pages).
Rohila, J. S., et al., "Improved Tandem Affinity Purification Tag and Methods for Isolation of Protein Heterocomplexes from Plants", The Plant Journal, 38, (2004), 172-181.
Santra, S., et al., "Luminescent Nanoparticle Probes for Bioimaging", Journal of Nanoscience and Nanotechnology, 4(6), (2004), 590-599.
Scholten, J. D., et al., "Novel Enzymatic hydrolytic degalogenation of a chlorinated aromatic", Science,253, (1991), 182-185.
Stroffekova, K., et al., "The protein-labeling reagent Flash-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins", Pflugers Arch., 442(6), (Sep. 2001), 859-66.
Stryer, Biochemistry, 3rd Ed., W. H. Freeman and Co., New York, 1988, pp. 757-758. *
Stryer, Lubert, "Biochemistry", Third Edition, (1988), 757-758, San Francisco, California.
The Second Symposium on Biological Imaging: New Dimensions in In Vivo Imaging, Lecturer Abstracts, obtained from www.promega-rd.info/bioimage2003/abstracts/lecture/default.asp, (2003), 5 Pages.
Tou, J. C, et al., "Kinetic Study of the Stabilities of Chloromethyl Methyl Ether and Bis(Chloromethyl) Ether in Humid Air", Analytical Chemistry, 46, (1974), 1866-1869.
Vincze, L., et al., "Three-Dimensional Trace Element Analysis by Confocal X-Ray Microfluorescence Imaging", Analytical Chemistry, 76, (2004), 6786-6791.
Wada, K., et al., "Codon usage tabulated from the GenBank genetic sequence data", Nucleic Acids Res., (May 1992), pp. 2111-2118.
Wang, H.-Z., et al., "Detection of Tumor Marker CA125 in Ovarian Carcinoma Using Quantum Dots", Acta Biochimica et Biophysica Sinica 2004, 36(10), (2004), 681-686.
Wayback machine, http://www.promega-rd.info.bioimage2003/abstracts/lecturer/default.asap, (Mar. 17, 2007).
Weissleder, R., et al., "Shedding Light Onto Live Molecular Target", Nature Medicine, 9(1), (2003), 123-128.
Wheeler, J. B, et al., "Conjugation of Haloalkanes by Bacterial and Mammalian Glutathione Transferases: Mono- and Vicinal Dihaloethanes", Chemical Research in Toxicology, 14, (2001), 1107-1117.
Winberg, J., "The catalytic triad in short-chain dehydrogenases", Dept. of Biochemistry, Institute of Medical Biology, University of Tromso, Abstract, (Nov. 2002), 2 pgs, Tromso, Norway.
Wolfgang, M. J, et al., "Nonhuman primate transgenesis: progress and prospects", Trends in Biotechnology, 20 (11), (Nov. 2002), 479-484.
Yang, G., et al., "Identification of Active Site Residues Essential to 4-Chlorobenzoyl-Coenzyme A Dehalogenase Catalysis by Chemical Modification and Site Directed Mutagenesis", Biochemistry, 35, (1996), 10879-10885.
Yang, M., et al., "Whole-Body Optical Imaging of Green Fluorescent Protein-Expressing Tumors and Metastases", PNAS, 97(3), (2000), 1206-1211.
Yokota, T., et al., "Purification and Properties of Haloalkane Dehalogenase from Corynebacterium sp. Strain m15-3", Journal of Bacteriology, 169(9), (Sep. 1987), 4049-4054.
Zawadzke, L., "Elimination of the hydrolytic water molecule in a class A beta-lactamase mutant: crystal structure and kinetics", Biochemistry, (Dec. 1996), pp. 16475-16482.
Zeph, L.R. et al., "Use of a biotinylated DNA probe to detect bacteria transduced by bacteriophage P1 in soil," Appl. Environ. Microbiol. (1989) 55(3):661-665.

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