USPP33073P3 - Cannabis plant named ‘MR2018002’ - Google Patents

Cannabis plant named ‘MR2018002’ Download PDF

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Publication number
USPP33073P3
USPP33073P3 US16/873,050 US202016873050V USPP33073P3 US PP33073 P3 USPP33073 P3 US PP33073P3 US 202016873050 V US202016873050 V US 202016873050V US PP33073 P3 USPP33073 P3 US PP33073P3
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average
plant
variety
color
cannabis plant
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US20200236830P1 (en
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Andrew Hand
Alan Graziano
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Aurora Cannabis Enterprises Inc
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Aurora Cannabis Enterprises Inc
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Assigned to AURORA CANNABIS ENTERPRISES INC. reassignment AURORA CANNABIS ENTERPRISES INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: GRAZIANO, ALAN, HAND, ANDREW
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/28Cannabaceae, e.g. cannabis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)

Definitions

  • Genus and species Cannabis sativa.
  • the present invention relates to a new and distinct Cannabis ( Cannabis sativa ) cultivar designated as ‘MR2018002’.
  • MR2018002 is a selection resulting from a controlled-cross between a male unnamed plant of Cannabis sativa variety of unknown background (unpatented) and a female unnamed plant of Cannabis sativa variety of unknown background (unpatented). The plants were grown in Markham, Ontario, Canada.
  • Seeds from the cross were sown and grown at an indoor Cannabis facility in Markham, Ontario, Canada.
  • the plants were flowered under standard indoor environment conditions with High Pressure Sodium lamps.
  • the plants were screened for a number of traits including tetrahydrocannabinol (THC) and cannabidiol (CBD) levels, yield, flowering time, disease resistance and flower morphology.
  • THC tetrahydrocannabinol
  • CBD cannabidiol
  • ‘MR2018002’ was first asexually propagated by apical stem cuttings approximately 10 cm long and having multiple auxiliary meristems, in Markham, Ontario, Canada. ‘MR2018002’ is stable and reproduces true to type in successive generations of asexual reproduction. This cultivar has increased resistance to microbial growth for enhanced cultivation and production of flower yield and extracts thereof.
  • This invention relates to a new and distinctive Cannabis cultivar designated as ‘MR2018002’.
  • ‘MR2018002’ exhibits about 6% THC and 13% CBD by dry weight. ‘MR2018002’ also exhibits microbial resistance.
  • FIG. 1 shows multiple, whole plant clones of ‘MR2018002’.
  • FIG. 2 shows a close-up view of an inflorescence and foliage of ‘MR2018002’.
  • Plants were flowered under standard indoor environmental conditions with High Pressure Sodium lamps. Clones were cut from healthy mothers and allowed to root for 14 to 18 days prior to being introduced to the flower room. The room was maintained at 18 hour days/6 hour nights for a week to allow the cuttings to establish adequate vegetative tissue. The floral transition was initiated by switching to 12 hour days and 12 hour nights. Flowering lasted 8-9 weeks before harvest, at which point samples were taken for chemical analysis, and yield was quantified. Morphological measurements were taken from plants aged 7 weeks post-floral transition.
  • Variety ‘MR2018002’ can also be distinguished from the variety ‘Girl Scout Cookies’ with respect to the morphological traits shown in Table 3.
  • the flowers produced by variety ‘MR2018002’ are denser and stickier than the flowers produced by ‘Girl Scout Cookies’.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A new cultivar of Cannabis plant named ‘MR2018002’ that is characterized by about 6% THC and 13% CBD by dry weight and resistance to microbial growth.

Description

Genus and species: Cannabis sativa.
Variety denomination: ‘MR2018002’.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of priority under 35 U.S.C. 199(f) to Canadian Plant Breeders' Rights Application Number 19-9708, which was filed for the instant plant variety on Jan. 18, 2019.
BACKGROUND OF THE INVENTION
The present invention relates to a new and distinct Cannabis (Cannabis sativa) cultivar designated as ‘MR2018002’.
‘MR2018002’ is a selection resulting from a controlled-cross between a male unnamed plant of Cannabis sativa variety of unknown background (unpatented) and a female unnamed plant of Cannabis sativa variety of unknown background (unpatented). The plants were grown in Markham, Ontario, Canada.
Seeds from the cross were sown and grown at an indoor Cannabis facility in Markham, Ontario, Canada. The plants were flowered under standard indoor environment conditions with High Pressure Sodium lamps. The plants were screened for a number of traits including tetrahydrocannabinol (THC) and cannabidiol (CBD) levels, yield, flowering time, disease resistance and flower morphology. An individual plant having about 6% THC and 13% CBD by dry weight and resistance to microbial growth was chosen to be ‘MR2018002’.
In September 2016, ‘MR2018002’ was first asexually propagated by apical stem cuttings approximately 10 cm long and having multiple auxiliary meristems, in Markham, Ontario, Canada. ‘MR2018002’ is stable and reproduces true to type in successive generations of asexual reproduction. This cultivar has increased resistance to microbial growth for enhanced cultivation and production of flower yield and extracts thereof.
BRIEF SUMMARY OF THE INVENTION
This invention relates to a new and distinctive Cannabis cultivar designated as ‘MR2018002’.
‘MR2018002’ exhibits about 6% THC and 13% CBD by dry weight. ‘MR2018002’ also exhibits microbial resistance.
BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying color photographs depict characteristics of ‘MR2018002.’ The colors shown are as true as can be reasonably obtained by conventional photographic procedures. The photographs are of plants aged 53 days post-floral transition and grown in flower rooms under standard cultivation methods in Bradford, Ontario Canada.
FIG. 1 shows multiple, whole plant clones of ‘MR2018002’.
FIG. 2 shows a close-up view of an inflorescence and foliage of ‘MR2018002’.
DETAILED BOTANICAL DESCRIPTION
The following is a detailed description of the new cultivar ‘MR2018002’.
‘MR2018002’ has not been tested under all possible environmental conditions. Phenotypic differences may be observed with variations in environment without any variance in genotype.
The traits of ‘MR2018002’ have been repeatedly observed and represent the distinguishing characteristics of ‘MR2018002’.
The data that follows was collected in Markham, Ontario, Canada.
Plants were flowered under standard indoor environmental conditions with High Pressure Sodium lamps. Clones were cut from healthy mothers and allowed to root for 14 to 18 days prior to being introduced to the flower room. The room was maintained at 18 hour days/6 hour nights for a week to allow the cuttings to establish adequate vegetative tissue. The floral transition was initiated by switching to 12 hour days and 12 hour nights. Flowering lasted 8-9 weeks before harvest, at which point samples were taken for chemical analysis, and yield was quantified. Morphological measurements were taken from plants aged 7 weeks post-floral transition.
Standard in-house developed nutrients were used throughout growth, and the plants were defoliated and pruned as necessary throughout the cycle.
In the following description, the color determination is in accordance with the 2017 Munsell Plant Tissue Color Book, except where general color terms of ordinary dictionary significance are used.
  • Classification:
      • Denomination.—‘MR2018002’.
      • Family.—Cannabacaeae.
      • Genus.—Cannabis.
      • Species.—Cannabis sativa.
      • Common name.—Marijuana.
  • Parentage: Male Cannabis sativa variety of unknown background and a female Cannabis sativa variety of unknown background.
  • Propagation: ‘MR2018002’ is asexually (clonally) propagated from vegetative cuttings. Roots are fine and well-branched. It takes approximately 14-18 days to produce a rooted young plant. Roots are fine and well-branched.
  • Plant:
      • Height.—65cm -75 cm.
      • Width.—Approx. 40 cm.
  • Stems:
      • Length/height.—50 cm-60 cm.
      • Width/diameter.—7 mm-9 mm.
      • Color.—2.5 GY 6/6, 5 RP 3/6.
      • Shape.—Tubular.
      • Texture.—Fibrous and Pubescent.
      • Lateral branch length.—25 cm-35 cm.
  • Foliage:
      • Type/form.—Palmately Compound.
      • Arrangement.—Alternate.
      • Attachment.—Petiolate.
      • Leaf width.—9 cm-14 cm.
      • Leaf length.—6 cm-15 cm.
      • Number of leaflets per leaf.—5-7.
      • Leaflet shape.—Lanceolate.
      • Leaflet length.—4 cm-14 cm.
      • Leaflet width.—1 cm-3 cm.
      • Leaflet margin.—Serrate.
      • Leaflet apex.—Acuminate.
      • Leaflet base.—Attenuate.
      • Leaflet color, upper surface.—7.5 GY 3/4, 7.5 GY 4/6.
      • Leaflet color, lower surface.—5 GY 5/4.
      • Venation pattern and description, upper and lower leaflet surfaces.—Pinnate.
      • Texture (both surfaces).—Pubescent, primarily on the abaxial side and along leaf vasculature.
      • Stipules.—Present, 2 per node on either side of a petiole, attenuated.
      • Fragrance.—Earthy, aromatic, herbal aroma.
  • Petiole:
      • Length.—1 cm-4.5 cm.
      • Diameter.—0.2 mm-0.29 mm.
      • Texture.—Fine, short non-glandular trichomes.
      • Color.—5 GY 6/6, 5 RP 3/6.
  • Inflorescence: The plant is a genetically female dioicous plant and, therefore, produces predominantly female flowers. There are no male-only plants. There is a very low incidence of hermaphroditism.
      • Blooming habit.—Short day photoperiod sensitive.
      • Blooming period.—51 days (average). Male flowering: male flowers are very rare; if present, typically develop between week 2 and 4 of general flowering.
      • Attachment.—Subsessile.
      • Bracts.—Covered with trichomes and resin glands with 2 to 3 stigmas.
      • Bract color.—5GY 7/8 (Munsell).
      • Trichomes.—Capitate-sessile, capitate-stalked, bulbous, and non-glandular (cystolith hairs); trichomes are present on almost all aerial organs but are present at highest concentration on female flowers (calyxes, bracts, etc.) as well as subtending intra-flower leaves.
      • Bract trichome and resin gland color.—Trichomes are clear, and will turn amber (approximately 10R 4/10 (Munsell)) during senescence.
      • Inflorescence color.—5 GY 5/8, 5 GY 5/10.
      • Number of inflorescences per plant.—18-35.
      • Diameter.—3 cm-6 cm.
      • Length.—4 cm-8 cm.
      • Inflorescence anthocyanin.—Absent for both female and male (very rare) flowers.
      • Average flower width.—3.7 cm.
      • Inflorescence fragrance.—Earthy, aromatic, herbal aroma.
  • Reproductive organs:
      • Pistils.—1.
      • Quantity (of stigmas) per flower.—2-4.
      • Stigma length.—0.4 cm-0.8 cm.
      • Stigma width.—0.7 mm-0.9 mm.
      • Stigma color.—2.5 GY 8/2, 7.5 YR 5/6.
  • THC and CBD content (quantified with a waters LC-MS/MS, running an acetonitrile:methanol:2-propanol gradient mobile phase through a raptor ACR-18, 2.7 um, 2.1×150 mm column):
      • Average % THC by dry weight.—6%.
      • Average % CBD by dry weight.—13%.
  • Yield:
      • Average flower yield.—642 g/m2 (grams dried, trimmed flower per m2 of growing space).
      • Average trim yield.—238 g/m2 (grams trichome rich leaf material per m2 that is removed during harvest/trimming).
  • Disease and insect/pest resistance: ‘MR2018002’ exhibits resistance to aerobic bacteria, yeast, mold and coliform bacteria as shown in Table 1.
TABLE 1
‘MR2018002’ resistance to aerobic bacteria, yeast,
mold and coliform bacteria.
Average Aerobic Bacteria 4407.5
Count (per plant, in colony
forming units (CFUs))
Average Yeast and Mold Count 2057.5
(per plant, in CFUs)
Average coliform bacteria 10
count (per plant, in CFUs)
COMPARISON WITH KNOWN VARIETY
A close variety of Cannabis known to the inventors is the commercial variety ‘Girl Scout Cookies’. ‘MR2018002’ can be distinguished from ‘Girl Scout Cookies’ by its increased resistance to microbial growth, as shown in Table 2:
TABLE 2
Comparison between ‘MR2018002’ and
‘Girl Scout Cookies’: Microbial resistance
Average
Average Yeast
# of Aerobic and Coliform
Cycles Bacteria Mold Bacteria
Variety Grown Count* Count** Count*
‘MR2018002’ 9 4407.5 2057.5 10
Girl Scout 15 59270 52450 760
‘Cookies’
**Aerobic Bacteria Count, Yeast and Mold Count and Coliform Bacteria Count were determined per plant, in colony forming units (CFUs).
Variety ‘MR2018002’ can also be distinguished from the variety ‘Girl Scout Cookies’ with respect to the morphological traits shown in Table 3.
TABLE 3
Comparison between ‘MR2018002’ and
‘Girl Scout Cookies’: Morphological traits
‘Girl Scout
Characteristic ‘MR2018002’ Cookies’
Overage 67.9 cm 69.6 cm 
Average Height
Average Height 68.7 cm  74 cm
Average # of 34.4 22.3
Nodes
Average  4.6 cm 6.6 cm
Internodal
Length
Average Flower  3.7 cm 5.3 cm
Width
In addition, the flowers produced by variety ‘MR2018002’ are denser and stickier than the flowers produced by ‘Girl Scout Cookies’.

Claims (1)

The invention claimed is:
1. A new and distinct cultivar of Cannabis plant named ‘MR2018002’ as described and illustrated herein.
US16/873,050 2019-01-18 2020-01-21 Cannabis plant named ‘MR2018002’ Active USPP33073P3 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/873,050 USPP33073P3 (en) 2019-01-18 2020-01-21 Cannabis plant named ‘MR2018002’

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA19-9708 2019-01-18
CAPBR19-9708 2019-01-18
US16/873,050 USPP33073P3 (en) 2019-01-18 2020-01-21 Cannabis plant named ‘MR2018002’

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US20200236830P1 US20200236830P1 (en) 2020-07-23
USPP33073P3 true USPP33073P3 (en) 2021-05-18

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Owner name: AURORA CANNABIS ENTERPRISES INC., CANADA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:HAND, ANDREW;GRAZIANO, ALAN;REEL/FRAME:055443/0181

Effective date: 20210226