USPP21030P3 - Kiwi plant named ‘Tsechelidis’ - Google Patents

Kiwi plant named ‘Tsechelidis’ Download PDF

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USPP21030P3
USPP21030P3 US11/987,178 US98717807V USPP21030P3 US PP21030 P3 USPP21030 P3 US PP21030P3 US 98717807 V US98717807 V US 98717807V US PP21030 P3 USPP21030 P3 US PP21030P3
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fruit
tsechelidis
hayward
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Christos Tsechelidis
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KARIPIDIS L - TSECHELIDOU E SP PARTNERSHIP dba - AGROHARA
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Karipidis L Tsechilidou ESP
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/08—Fruits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy

Definitions

  • Kiwi plants have been cultivated outside their native China for over one hundred years.
  • Known varieties or cultivars include Hayward, Meteor, Hort16A, Abbott, Tomua, Jade Moon, Bruno, Monty, Matua and Kuimi.
  • Hayward is the most popular variety worldwide. Kiwi plants are now commercially grown in New Zealand, Italy, Chile, France, Greece, Japan, China and the United States.
  • Kiwi plants are commercially grown for their oblong or oval fruit, having brown skin covered in short hairs.
  • the flesh, firm until fully ripe, is glistening, bright green or sometimes yellow, brownish or off-white, except for the white, succulent center from which radiate many fine, pale lines. Between these lines are scattered minute dark-purple or nearly black seeds, unnoticeable in eating.
  • Kiwi plants may be propagated by seed, grafting or cutting.
  • the present invention relates to a new and distinctive kiwi variety characterized by very large, oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features.
  • the size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi.
  • the new variety designated ‘Tsechelidis’ was derived from the ‘Hayward’ variety in Episkopi, Imathia, Greece and has been asexually reproduced by cutting, among other methods.
  • ‘Tsechelidis’ is further distinguished by the nutritional characteristics of the fruit as well as the qualitative characteristics. Furthermore, a molecular genetic analysis distinguishes ‘Tsechelidis’ from ‘Hayward’ as indicated by several polymorphisms in known alleles.
  • FIG. 1 shows a typical leaf of ‘Tsechelidis’.
  • FIG. 2 shows typical flowers of ‘Tsechelidis’.
  • FIG. 3 shows a typical flower of ‘Tsechelidis’ as compared to a typical flower of ‘Hayward’.
  • FIG. 4 shows typical fruit of ‘Tsechelidis’ on the vine.
  • FIG. 5 shows typical fruit of ‘Tsechelidis’ on the vine as compared to typical fruit of ‘Hayward’ on the vine.
  • FIG. 6 shows typical fruit of ‘Tsechelidis’ with stems attached.
  • FIG. 7 shows a cross-section and a stylar end view of a typical fruit of ‘Tsechelidis’
  • FIG. 8 shows a cross-section and a side view of a typical fruit of ‘Tsechelidis’.
  • FIG. 9 shows typical fruit of ‘Tscehelidis’ as compared to typical fruit of ‘Hayward’.
  • the present invention is a new and distinct variety of Actinidia deliciosa plants having the several characteristics that distinguish the variety from other kiwi plants, particularly the ‘Hayward’ variety.
  • the new variety ‘Tsechelidis’ was discovered in Episkopi, Imathia, Greece, when the inventor planted seeds from selected Hayward plants with the purpose of obtaining a group of male plants. From this original group of twenty plants, a single plant was identified as female. During its first harvest in 1994, the female plant bore unusually large fruit as compared to plants of the ‘Hayward’ variety. The female plant was monitored for the next two harvests and consistently bore the unusually large fruit, as well as other features which distinguished it from ‘Hayward’. The female plant was then used to propagate the ‘Tseehelidis’ variety by asexual reproduction beginning in 1996.
  • the ‘Tsechelidis’ variety has been continuously asexually reproduced by cuttings from 1996 through 2007. The distinguishing characteristics of the variety continue to run true in the propagated plants, as shown by about 1000 plants covering 1 ⁇ 2 hectare. Furthermore, no changes have appeared in the new variety when grafted on ‘Hayward’. In addition to cuttings, the new variety can be asexually reproduced by grafting to rootstocks of A. deliciosa .
  • Male pollenizers suitable for ‘Tsechelidis’ may include, but are not limited to, ‘Mania’. At this time the ‘Tsechelidis’ variety is being tested for self-pollenization by the University of Volvos, Greece. According to preliminary results, ‘Tsechelidis’ is characterized by up to 75% self-pollenization as compared to 2% for ‘Hayward’.
  • ‘Tsechelidis’ should be cultivated in areas that are not affected by spring frost. Temperatures of less than ⁇ 2° C. will damage the tender shoots of the plant and suspending growth or reduce the setting process. Also, frost in early autumn to late October may damage fruit situated on the vine. The presence of strong wind, particularly in May, may result in considerable decrease of flowers borne by the plant.
  • the chart below describes the physical differences between ‘Tsechelidis’ and the known ‘Hayward’ variety.
  • the Horticultural terminology applied below is used in accordance with revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6 — Actinidia 2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg_98_6. pdf).
  • the shoots of ‘Tsechelidis’ grow more vigorously than ‘Hayward’, though there is no difference with respect to resilience against pest and diseases.
  • the fruits of ‘Tsechelidis’ are more oblong than ‘Hayward’ (the ratio of fruit length/width is 1.41 and 1.24 respectively).
  • the fruits of ‘Tsechelidis’ are larger and more uniform in size. ‘Tsechelidis’ do no require thinning absent defective fruit, unlike ‘Hayward’. Additionally, due to the larger fruit size, any decrease that may be caused by low temperatures during the flowering season or poor pollination of the fruits will not affect the marketability of the fruits, in contrast with ‘Hayward’.
  • the typical size of the leaves includes a stalk length of 12–14 cm, a length from stalk to apex of 18–20 cm and a leaf width of 16-18 cm.
  • the typical flower diameter is 5–7 cm.
  • the flowers are typically characterized by 5-6 sepals, 6-8 petals and 35-45 styles.
  • the stalk length of the flowers typically ranges from 6–9 cm.
  • the fruit length typically averages about 7.96 cm.
  • the fruit width typically ranges from about 5.15 cm (small width) to about 5.71 cm (large width).
  • the fruit weight typically ranges from 150-170 g.
  • ‘Tsechelidis’ has a greater density of buds in each stem than ‘Hayward’, whereby each stem, which has 13 buds, produces about 10 kg of fruit. Therefore, each ‘Tsechelidis’ tree, having about 15-18 stems, yields about 140-150 kg of fruit.
  • the high yield, and large size and uniformity of the fruit of ‘Tsechelidis’, as compared to the ‘Hayward’, are significant advantages, particularly with regard to reducing production costs. This data was taken during a harvest affected by adverse weather during the growing season in Imathia, Greece.
  • ‘Tsechelidis’ The fruit of ‘Tsechelidis’ ripen 7-10 days earlier than ‘Hayward’, which is shown by the fruit's lower resistance to pressure and their slight superiority in soluble solids (° Brix) during harvest. Despite the earlier ripening, the resistance to pressure of ‘Tsechelidis’ after two months of refrigerated storage was the same as ‘Hayward’.
  • the DNA analysis was performed as follows: Repeatable samples of four genotypes were used (commercial clones of ‘Hayward’ (one female, one male) and ‘Tsechelidis’ (one female, one male)). Leaf samples of 0.3 g per genotype was used for DNA extraction process according to a modified cetyltrimethylammonium bromide (CTAB) method. The extract DNA was quantified on agarose gel by comparison with report samples (DNA marker). The DNA quality and quantity was tested to ensure accuracy of the molecular genetic analysis.
  • CTAB cetyltrimethylammonium bromide
  • primer pairs Thirteen known primer pairs were used for amplifying dinucleotide tandems AG/CT and AC/GT.
  • the primer pairs used were: UDK 96-022, UDK 97-402, UDK 99-152, UDK 96-053, UDK 97-411, UDK 96-030, UDK 96-001, UDK 96-037, UDK 96-034.
  • the primers were developed in a bilateral European Union International Cooperation with Developing countries (INCO-DC) project performed by
  • UDK 96-001, UDK 96-022, UDK 96-030, UDK 96-034, UDK 96-037, UDK 97-402, UDK 97-406, UDK 97-407 and UDK-41 1 are all published in Huang et al., Microsatellite DNA in Actinidia chinesis : isolation, characterization, and homology in related species. Theor. Appl. Genet. (1998) 97: 1269-1278.
  • UDK 96-037 a and b listed below indicate the two polymorphic loci amplified in the same gel for the same primer UDK 96-037.
  • UDK 96-053 and UDK 99-152 are published in Korkovelos et al. Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species. Scientica Horticulturae 116 (2008) 305-310.
  • UDK 96-092 and UDK 99-168 are also know primers as disclosed by Korkovelos et al. Screening microsatellites for their effectiveness to identify and differentiate among Actinidia Genotypes . Acta Hort. 610 (2003) 357-363.
  • the PCR products were separated in 6% polyacrylamide gels 1.5 mm thick. Band visualization was made with silver nitrate. The results of the study indicated that at least seven out of 13 primer pairs were polymorphic. Therefore, the study provides grounds for distinguishing between genetic material from ‘Tsechelidis’ as compared to ‘Hayward’.
  • the polymorphic primer pairs are described in the table below according to the presence and absence of alleles of the same molecular weight.

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  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A new and distinctive variety of the kiwi plant, Actinidia deliciosa, named ‘Tsechelidis’ is described. The new variety is characterized by very large oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features. The size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi.

Description

Latin name of the genus and species of the plant claimed:
Actinidia deliciosa.
Variety denomination: ‘Tsechelidis’.
BACKGROUND OF THE INVENTION
Kiwi plants have been cultivated outside their native China for over one hundred years. Known varieties or cultivars include Hayward, Meteor, Hort16A, Abbott, Tomua, Jade Moon, Bruno, Monty, Matua and Kuimi. Hayward is the most popular variety worldwide. Kiwi plants are now commercially grown in New Zealand, Italy, Chile, France, Greece, Japan, China and the United States.
Kiwi plants are commercially grown for their oblong or oval fruit, having brown skin covered in short hairs. The flesh, firm until fully ripe, is glistening, bright green or sometimes yellow, brownish or off-white, except for the white, succulent center from which radiate many fine, pale lines. Between these lines are scattered minute dark-purple or nearly black seeds, unnoticeable in eating.
Kiwi plants may be propagated by seed, grafting or cutting.
SUMMARY OF THE INVENTION
The present invention relates to a new and distinctive kiwi variety characterized by very large, oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features. The size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi. The new variety designated ‘Tsechelidis’ was derived from the ‘Hayward’ variety in Episkopi, Imathia, Greece and has been asexually reproduced by cutting, among other methods.
‘Tsechelidis’ is further distinguished by the nutritional characteristics of the fruit as well as the qualitative characteristics. Furthermore, a molecular genetic analysis distinguishes ‘Tsechelidis’ from ‘Hayward’ as indicated by several polymorphisms in known alleles.
BRIEF DESCRIPTION OF THE PHOTOGRAPHS
The accompanying color photographs of ‘Tsechelidis’ show the new variety as well as comparisons of the ‘Tsechelidis’ variety to the ‘Hayward’ variety.
FIG. 1 shows a typical leaf of ‘Tsechelidis’.
FIG. 2 shows typical flowers of ‘Tsechelidis’.
FIG. 3 shows a typical flower of ‘Tsechelidis’ as compared to a typical flower of ‘Hayward’.
FIG. 4 shows typical fruit of ‘Tsechelidis’ on the vine.
FIG. 5 shows typical fruit of ‘Tsechelidis’ on the vine as compared to typical fruit of ‘Hayward’ on the vine.
FIG. 6 shows typical fruit of ‘Tsechelidis’ with stems attached.
FIG. 7 shows a cross-section and a stylar end view of a typical fruit of ‘Tsechelidis’
FIG. 8 shows a cross-section and a side view of a typical fruit of ‘Tsechelidis’.
FIG. 9 shows typical fruit of ‘Tscehelidis’ as compared to typical fruit of ‘Hayward’.
 DETAILED DESCRIPTION OF THE INVENTION
The present invention is a new and distinct variety of Actinidia deliciosa plants having the several characteristics that distinguish the variety from other kiwi plants, particularly the ‘Hayward’ variety.
The new variety ‘Tsechelidis’ was discovered in Episkopi, Imathia, Greece, when the inventor planted seeds from selected Hayward plants with the purpose of obtaining a group of male plants. From this original group of twenty plants, a single plant was identified as female. During its first harvest in 1994, the female plant bore unusually large fruit as compared to plants of the ‘Hayward’ variety. The female plant was monitored for the next two harvests and consistently bore the unusually large fruit, as well as other features which distinguished it from ‘Hayward’. The female plant was then used to propagate the ‘Tseehelidis’ variety by asexual reproduction beginning in 1996.
The ‘Tsechelidis’ variety has been continuously asexually reproduced by cuttings from 1996 through 2007. The distinguishing characteristics of the variety continue to run true in the propagated plants, as shown by about 1000 plants covering ½ hectare. Furthermore, no changes have appeared in the new variety when grafted on ‘Hayward’. In addition to cuttings, the new variety can be asexually reproduced by grafting to rootstocks of A. deliciosa.
Male pollenizers suitable for ‘Tsechelidis’ may include, but are not limited to, ‘Mania’. At this time the ‘Tsechelidis’ variety is being tested for self-pollenization by the University of Volvos, Greece. According to preliminary results, ‘Tsechelidis’ is characterized by up to 75% self-pollenization as compared to 2% for ‘Hayward’.
‘Tsechelidis’ should be cultivated in areas that are not affected by spring frost. Temperatures of less than −2° C. will damage the tender shoots of the plant and suspending growth or reduce the setting process. Also, frost in early autumn to late October may damage fruit situated on the vine. The presence of strong wind, particularly in May, may result in considerable decrease of flowers borne by the plant.
Distinguishing Characteristics from Known Varieties
The following is a comparison of the fruit production an orchard of ‘Tsechelidis’ as compared with cultivated ‘Hayward’ situated in the area of Episkopi-Anthemion in the prefecture of Imathia, Greece. At the time of this study (2006) the ‘Tsechelidis’ orchard was five years old.
The listed observations, measurements and assessments were made in the following categories:
    • 1. Plant and fruit characteristics
    • 2. Quantitative production data (yield, number of fruits/plants and average fruit weight).
    • 3. Level of nutritional elements in leaves and fruit.
    • 4. Qualitative fruit characteristics (resistance to pressure, pH, soluble solids (° Brix), vitamin C, acids, sugars, Thiault index and dry matter).
    • 5. Plant/Fruit disease and pest resistance/susceptibility
      1. Plant and Fruit Characteristics:
The chart below describes the physical differences between ‘Tsechelidis’ and the known ‘Hayward’ variety. The Horticultural terminology applied below is used in accordance with revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6 —Actinidia 2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg_98_6. pdf).
TABLE 1
UPOV Characteristics for
comparison of varieties ‘HAYWARD’ ‘TSECHELIDIS’
Plant: vigor Medium Strong
Leaf blade: shape Broad ovate Very broad ovate
Leaf blade: shape of apex Acute Acuminate
Leaf blade: green color of Medium Dark
upper side
Petal: curvature of apex Strongly Very strongly expressed
expressed
Fruit: size Large Very large
Fruit: general shape Ellipsoid Oblong
Fruit: general shape of stylar Flat Between slightly
end depressed and flat
Fruit: shape of shoulder at Rounded Squared
stalk end
Fruit: type of hairiness Hirsute Downy
Time of beginning of Late Medium
flowering
The shoots of ‘Tsechelidis’ grow more vigorously than ‘Hayward’, though there is no difference with respect to resilience against pest and diseases. The fruits of ‘Tsechelidis’ are more oblong than ‘Hayward’ (the ratio of fruit length/width is 1.41 and 1.24 respectively). Furthermore, the fruits of ‘Tsechelidis’ are larger and more uniform in size. ‘Tsechelidis’ do no require thinning absent defective fruit, unlike ‘Hayward’. Additionally, due to the larger fruit size, any decrease that may be caused by low temperatures during the flowering season or poor pollination of the fruits will not affect the marketability of the fruits, in contrast with ‘Hayward’.
In addition to the distinguishing features between ‘Tsechelidis’ and ‘Hayward’ listed above, the following characteristics were identified by the European Community Plant Variety Office (CPVO). Again the terminology is presented according to the revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6—Actinidia 2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg_98_6.pdf). The UPOV characteristics are botanical terms known in the art for description plant varieties.
TABLE 2
UPOV State of
No. Characteristics Expression
5 Young shoot: Hairiness present
6 Young shoot: Density of hair medium
7 Young shoot: Type of hairiness hirsute
8 Young shoot: Anthocyanin coloration of medium
growing tip
9 Stem: Thickness medium
11 Stem: Roughness of bark medium-rough
12 Stem: Hairiness present
13 Stem: Density of hair medium
14 Stem: Type of hairiness bristly
15 Stem: Size of lenticels medium
16 Stem: Number of lenticels medium
19 Stem: Size of bud support small to medium
20 Stem: Profile of proximal face of bud convex
support (if sloping)
21 Stem: Presence of bud cover present
22 Stem: Size of hole in bud cover medium
23 Stem: Leaf sear shallow
26 Leaf blade: Shape very broad ovate
27 Leaf blade: Shape of apex acuminate
28 Leaf blade: Arrangement of basal lobes slightly apart
29 Leaf blade: Hair on upper side medium
30 Leaf blade: Hair on lower side medium
31 Leaf blade: Puckering/Blistering on medium
upper side
34 Leaf blade: Presence of variegation absent
37 Leaf: Ratio petiole length/blade length large
38 Petiole: Density of hair medium-dense
39 Petiole: Anthocyanin coloration on medium
tipper side
40 Flower bud: anthocyanin coloration of medium
protruding petal end
41 Inflorescence: Predominant number of one
flowers
42 Flower stalk: Length medium-long
43 Flower stalk: Density of hair medium
44 Flower stalk: Length of hair medium
45 Flower: Number of sepals 6
47 Sepal: Density of hair sparse
48 Sepal: Length of hair medium-short
49 Flower: Diameter very large
50 Flower: Arrangement of petals (viewed overlapping
from beneath)
51 Petal: Curvature of apex strongly expressed
52 Petal: Type of coloration (adaxial side) single colored
54 Petal: Different shades of color absent
60 Styles: Number many
62 Styles: Attitude erect and horizontal
63 Fruit: Size very large
64 Fruit: General shape oblong
65 Fruit: Shape in cross-section (at median) elliptic
66 Fruit: General shape at stylar end slight depressed
and flat
67 Fruit: Presence of calyx ring weakly expressed
68 Fruit: Shape of shoulder at stalk end squared
69 Fruit: Length of stalk long
70 Fruit: Ratio stalk length/fruit length large
71 Fruit: Persistence of sepals present
72 Fruit: Conspicuousness of lenticels on skin inconspicuous
74 Fruit: Hairiness of skin present
75 Fruit: Density of hair medium
76 Fruit: Type of hairiness downy
77 Fruit: Distribution of hairs evenly spread
79 Fruit: Adherence of hair to skin medium-strong
(when rubbed)
84 Fruit: Diameter of core relative to fruit medium to large
85 Fruit: General shape of core (in cross transverse elliptic
section)
86 Fruit: Fluting of core (in cross section) present
88 Fruit: Sweetness medium-low
89 Fruit: Acidity medium
90 Time of vegetative bud burst medium
91 Time of beginning flowering medium-late
92 Time of maturity for harvest medium-late
In addition to the above listed characteristics set forth according to the UPOV guidelines, the following measurements are typical of ‘Tsehedilids.’ The typical size of the leaves includes a stalk length of 12–14 cm, a length from stalk to apex of 18–20 cm and a leaf width of 16-18 cm. The typical flower diameter is 5–7 cm. The flowers are typically characterized by 5-6 sepals, 6-8 petals and 35-45 styles. The stalk length of the flowers typically ranges from 6–9 cm.
With regards to the fruit, the fruit length typically averages about 7.96 cm. The fruit width typically ranges from about 5.15 cm (small width) to about 5.71 cm (large width). The fruit weight typically ranges from 150-170 g.
The following color description has been provided according to the R.H.S. Colour Chart.
TABLE 3
Characteristic Botanical Features Color according to RHS Colour Chart
Stem: Color of shoot on sunny side 178A-Greyed-Red Group
Stem: Color of lenticels 177C-Greyed-Orange Group
Leaf blade: Color of upper side 137B-Green Group
Leaf blade: Color of lower side 146C-YelIow-Green Group
Sepal: General color 200D-Brown Group
Petal: Main color on adaxial side 155D-White Group
Filament: Color 155C-White Group
Anther: Color 13C-Yellow Group
Styles: Color 158B-YelIow-White Group
Fruit: Color of skin 199A-Grey-Brown Group
Fruit: Color of hairs 199D-Grey-Brown Group
Fruit: Color of skin at maturity for 199A-GreyBrown Group
consumption
Fruit: Color of outer pericarp 141C-Green Group
Fruit: Color of inner pericarp 141C-Green Group
Fruit: Color of core 157D-Green-White Group

2. Quantitative Production Data:
TABLE 4
‘HAY- Significance
Parameter ‘TSECHELIDIS’ WARD’ level (P)
Total number of fruits/plant 250 279 P > 0.05
Number of marketable 249 222 P > 0.05
fruits
Rate of marketable 99.6 79.6 —
fruits (%)
Total yield (kg/plant) 41.9 29.9 P > 0.05
Yield of marketable fruits 41.6 25.1 0.001 <
(kg/plant) P < 0.01
Yield of marketable fruits 99.3 83.9 —
(%)
Average weight of market- 167.0 114.5 P < 0.001
able fruits (g)
Average weight of non- 237.0 88.8 P > 0.001
marketable fruits (g)
P > 0.05 signifies a statistically insignificant difference.
‘Tsechelidis’ has a greater density of buds in each stem than ‘Hayward’, whereby each stem, which has 13 buds, produces about 10 kg of fruit. Therefore, each ‘Tsechelidis’ tree, having about 15-18 stems, yields about 140-150 kg of fruit. The high yield, and large size and uniformity of the fruit of ‘Tsechelidis’, as compared to the ‘Hayward’, are significant advantages, particularly with regard to reducing production costs. This data was taken during a harvest affected by adverse weather during the growing season in Imathia, Greece.
3. Level of Nutritional Elements The following is table that shows the statistically significant differences in nutritional elements between ‘Tsechelidis’ and ‘Hayward’.
TABLE 5
Parameter ‘TSECHELIDIS’ ‘HAYWARD’
Leaves: nitrogen level 1.95% 2.53%
Fruit skin: phosphorus 0.13% 0.08%
Fruit skin: potassium 2.35% 1.95%
Fruit skin: magnesium 0.08% 0.06%
Fruit skin: manganese 12.6 ppm 8.0 ppm
Fruit flesh: nitrogen 0.76% 0.95%
Fruit flesh: phosphorus 0.13% 0.16%
Fruit flesh: manganese 10.3 ppm 6.2 ppm
Fruit flesh: copper 6.79 ppm 10.51 ppm
Fruit flesh: proportion of 2.30 2.71
N/Ca

4. Qualitative fruit characteristics The following tables show specific qualitative fruit characteristics between ‘Tsechelidis’ and ‘Hayward’. The first table lists measurements of fruit immediately after harvest. The second table lists measurements taken of fruit held in refrigerated storage for two months after harvest.
TABLE 6
Significance
Parameter ‘TSECHELIDIS’ ‘HAYWARD’ level (P)
Measurements Taken During Harvest
Resistance to 23.0 27.0 0.001 <
pressure (lb/in2) P < 0.01
Flesh pH 3.34 3.25 0.0l < P < 0.05
Soluble solids 7.30 6.70 P > 0.05
(°Brix)(%)
Vitamin C (mg/100 79.2 37.8 P < 0.00l
g fresh weight)
Measurements Taken During Harvest
Malic acid (g/l) 4.5 4.0 P > 0.05
Sugars (g/l) 62.8 57.2 0.01 <
P < 0.05
Thiault index 107.7 97.6 0.01 <
P < 0.05
Dry matter (%) 15.30 15.82 P > 0.05
TABLE 7
Measurements Taken Two (2) Months After Harvest
Significance
Parameter ‘TSECHELIDIS’ ‘HAYWARD’ level (P)
Resistance to 10. 10.9 P > 0.05
pressure (lb/in2)
Flesh pH 3.32 3.41 P < 0.001
Soluble solids 13.6 13.0 P > 0.05
(°Brix) (%)
Vitamin C (mg/100 80.2 38.3 P < 0.001
g fresh weight)
Malic acid (g/l) 4.8 4.5 P > 0.05
Sugars (g/l) 84.4 80.0 P > 0.05
Thiault index 132.4 125.0 P > 0.05
Based on the above information, the following distinctions may be drawn between ‘Tsechelidis’ and ‘Hayward’. The fruit of ‘Tsechelidis’ ripen 7-10 days earlier than ‘Hayward’, which is shown by the fruit's lower resistance to pressure and their slight superiority in soluble solids (° Brix) during harvest. Despite the earlier ripening, the resistance to pressure of ‘Tsechelidis’ after two months of refrigerated storage was the same as ‘Hayward’.
5. Plant/fruit disease and pest resistance/susceptibility
There is no difference between ‘Tsechelidis’ and ‘Hayward’ with respect to resilience against pest and diseases. There are no other observed characteristics specific to plant/fruit disease and pest resistance/susceptibility.
Molecular Genetic Analysis
A molecular genetic analysis was conducted by Dr. Athanasios Mavromatis, Professor of Genetics & Plant Breeding (University of Thessaly, School of Agricultural Services) comparing ‘Tsechelidis’ with ‘Hayward’ based on known microsatellite DNA markers using PCR. The method is recognized as an accurate and repeatable molecular analysis for Actinidia. Huang, W.G., Cipriani, G., Morgante, M., Testolin, R. (1998) Microsatellite DNA in Actinidia chinensis: isolation, characterization, and homology in related species. Theor. Appl. Genet. 97 (8): 1269-1278.
The DNA analysis was performed as follows: Repeatable samples of four genotypes were used (commercial clones of ‘Hayward’ (one female, one male) and ‘Tsechelidis’ (one female, one male)). Leaf samples of 0.3 g per genotype was used for DNA extraction process according to a modified cetyltrimethylammonium bromide (CTAB) method. The extract DNA was quantified on agarose gel by comparison with report samples (DNA marker). The DNA quality and quantity was tested to ensure accuracy of the molecular genetic analysis.
Thirteen known primer pairs were used for amplifying dinucleotide tandems AG/CT and AC/GT. The primer pairs used were: UDK 96-022, UDK 97-402, UDK 99-152, UDK 96-053, UDK 97-411, UDK 96-030, UDK 96-001, UDK 96-037, UDK 96-034. UDK 99-168, UDK 96- 092, UDK 97-406 and UDK 97-407. The primers were developed in a bilateral European Union International Cooperation with Developing Countries (INCO-DC) project performed by
University of Udine, Italy; Chinese Agricultural University, Beijing China; INRA, France; and University of Thessaly, Greece.
The sequence of all of the list primers are known and published the prior art. For example the sequences of UDK 96-001, UDK 96-022, UDK 96-030, UDK 96-034, UDK 96-037, UDK 97-402, UDK 97-406, UDK 97-407 and UDK-41 1 are all published in Huang et al., Microsatellite DNA in Actinidia chinesis: isolation, characterization, and homology in related species. Theor. Appl. Genet. (1998) 97: 1269-1278. UDK 96-037 a and b listed below indicate the two polymorphic loci amplified in the same gel for the same primer UDK 96-037. The sequences of UDK 96-053 and UDK 99-152 are published in Korkovelos et al. Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species. Scientica Horticulturae 116 (2008) 305-310. UDK 96-092 and UDK 99-168 are also know primers as disclosed by Korkovelos et al. Screening microsatellites for their effectiveness to identify and differentiate among Actinidia Genotypes. Acta Hort. 610 (2003) 357-363.
The PCR products were separated in 6% polyacrylamide gels 1.5 mm thick. Band visualization was made with silver nitrate. The results of the study indicated that at least seven out of 13 primer pairs were polymorphic. Therefore, the study provides grounds for distinguishing between genetic material from ‘Tsechelidis’ as compared to ‘Hayward’.
The polymorphic primer pairs are described in the table below according to the presence and absence of alleles of the same molecular weight.
TABLE 8
DNA primer/alleles ‘TSECHELIDIS’ ‘HAYWARD’
99-152
97-411 + −
96-030
  96-037a − +
 96-037b + −
96-034 + −
96-092 + −
97-406 − +
The genetic difference confirmed through diverse binding patterns indicate that the ‘Tsechelidis’ variety is genetically distinct from ‘Hayward’.

Claims (1)

1. A new and distinct variety of Actinidia deliciosa plant named ‘Tseehelidis’ substantially as shown and described.
US11/987,178 2007-11-28 2007-11-28 Kiwi plant named ‘Tsechelidis’ Active 2027-12-22 USPP21030P3 (en)

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Non-Patent Citations (3)

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Title
Huang, et al. Microsatellite DNA in Actinidia chinensis: isolation, characterisation, and homology in related species, Theor Appl Genet (1998) 97: 1269-1278.
Korkovelos, et al., Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species, Scientia Horticulturae 116 (2008) 305-310.
Korkovelos, et al., Screening Microsatellites for their Effectiveness to Identify and Differentiate among Actinidia Genotypes, Proc. IS on Kiwifruit, Ed. H. Huang, Acta Hort 610, ISHS pp. 357-363, 2007.

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