USPP11570P - Zoysiagrass plant named `Crowne` - Google Patents

Zoysiagrass plant named `Crowne` Download PDF

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USPP11570P
USPP11570P US09/076,973 US7697398V US11570P US PP11570 P USPP11570 P US PP11570P US 7697398 V US7697398 V US 7697398V US 11570 P US11570 P US 11570P
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crowne
zoysiagrass
meyer
ntep
internode
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Milton Charles Engelke
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/469—Zoysia

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  • the present invention relates to a new and distinct asexually reproduced variety of perennial zoysiagrass (Zoysia japonica).
  • This invention relates to a new and distinct perennial zoysiagrass cultivar identified as ⁇ Crowne ⁇ zoysiagrass (herein referred to as ⁇ Crowne ⁇ ), that was tested as DALZ8512.
  • ⁇ Crowne ⁇ a Zoysia japonica, is a chance hybrid of the maternal close ⁇ Z20 ⁇ (unpatented), obtained from Beltsville, MD in 1981, with an unknown pollen source from a zoysiagrass germplasm field nursery at TAES--Dallas.
  • ⁇ Crowne ⁇ has been vegetatively propagated by stolons and rhizomes in Dallas, Tex., and is uniform in growth expression through successive generations.
  • the new variety of zoysiagrass of the present invention is named ⁇ Crowne ⁇ .
  • FIG. 1 is a photograph of the leaf blade and ligule of ⁇ Crowne ⁇ .
  • FIG. 2 is a DNA fingerprint of ⁇ Crowne ⁇ in contrast to ⁇ Meyer ⁇ (unpatented) zoysiagrass.
  • ⁇ Crowne ⁇ was characterized in greenhouse and field conditions. ⁇ Crowne ⁇ is a unique variety of zoysiagrass developed as described above. ⁇ Crowne ⁇ was identified as being unique and desirable and was then propagated by cutting of stolons and rhizomes, rooting them in soil, and planting of the rooted material to provide planting stock for studying performance and for comparison of morphological characters after propagation. ⁇ Crowne ⁇ has been propagated by sod, plugs, sprigs, stolons and rhizomes in greenhouse and field studies by hand and mechanical propagation in Dallas, Tex. All distinguishing and defining characteristics are reliably reproduced in stable and firmly fixed form by such means through succeeding generations. Seed reproduction with self-fertility is not common in the Zoysia sp. No seedling establishment from ⁇ Crowne ⁇ has been noticed in either greenhouse or field studies.
  • ⁇ Crowne ⁇ spreads by stolons and rhizomes. It has an intermediate to rapid establishment rate and has low water use requirements.
  • sprig (stolons) or plug plantings are made in early May, the area should achieve 50-60% average in Dallas, Tex. in 90-120 days, with complete coverage in 190-210 days, in comparison to ⁇ Meyer ⁇ zoysiagrass which will require 360-400 days for full coverage.
  • the stolons of ⁇ Crowne ⁇ have a mean internode length of 17.7 mm between the second and third nodes, with a mean internode width of 0.9 mm and node diameter of 1.1; internode length between the fourth to fifth internode was 12.0 mm with a stolon width of 1.8 mm (Tables 1,2). This creates a proportionally short-thick internode unique to ⁇ Crowne ⁇ .
  • the stolons of ⁇ Crowne ⁇ root adventitiously at the nodes. Color notations of plant tissues were based on the Munsell Color Charts for Plant Tissues, Munsell Color, Baltimore, Md., 1977. Light quality, photoperiod, and general growth of the plants affect color notations.
  • the internode stolon color of ⁇ Crowne ⁇ stolons exposed to full sun is 10R 3/2.
  • Leaf blades of ⁇ Crowne ⁇ are rolled in the bud and are flat and stiff.
  • the leaf blade length of ⁇ Crowne ⁇ is from 72.7 to 126.6 mm and 2.96 to 3.23 mm in width (Tables 3,4).
  • the leaf sheath of ⁇ Crowne ⁇ is distinct from ⁇ Meyer ⁇ (Table 5).
  • the hairs on the abaxial leaf surface of ⁇ Crowne ⁇ are sparse in number and are approximately 0.9 mm long. Measured under greenhouse conditions in January 1996, the genetic, adaxial leaf color of ⁇ Crowne ⁇ is 2.5G 4/4 with ⁇ El Toro ⁇ (U.S. Plant Pat. No.
  • ligule of ⁇ Crowne ⁇ is a fringe of silky hairs, approximately 2.5 mm in length for the longest hairs.
  • Thatch development is a function of age of stand, types of soils and other environmental and cultural conditions. Thatch development was determined in 3 year old field plantings comparing eight commercially available cultivars. ⁇ Emerald ⁇ , ⁇ Miyako ⁇ (U.S. Plant Pat. No. 10,187) and ⁇ Zeon ⁇ (unpatented) produced the greatest depth of thatch (26.3 mm); ⁇ Crowne ⁇ , ⁇ Palisades ⁇ and ⁇ De Anza ⁇ produced an intermediate amount of thatch (25, 23 and 23 mm, respectively), and ⁇ Cavalier ⁇ (U.S. Plant Pat. No. 10,778) and ⁇ Meyer ⁇ produced the least amount of thatch (20 and 19 mm, respectively).
  • ⁇ Crowne ⁇ has 5.0 RP 3/4 colored anthers and white colored stigmas, undistinguished in shade of color.
  • the inflorescence of ⁇ Crowne ⁇ is a terminal spike-like raceme, with spikelets on short pedicels.
  • ⁇ Crowne ⁇ has a mean length floral region of 23.1 mm with a mean of 25.3 florets per raceme.
  • the chromosome number of ⁇ Crowne ⁇ is 40.
  • ⁇ Crowne ⁇ shows intermediate resistance to the zoysiagrass mite (Table 9).
  • the varieties ⁇ Meyer ⁇ , ⁇ Belair ⁇ (unpatented), and most experimental zoysiagrasses are very susceptible to the mite. This mite has been found in Maryland, Texas, Florida, and all extensive zones of use for zoysiagrasses.
  • ⁇ Crowne ⁇ is distinguished from other zoysiagrass by its coarse texture, thick stolons, and intermediate to good stolon and rhizome production. It has moderate to good shade tolerance and low water use needs and is highly competitive under natural environmental conditions typical of north central Texas. ⁇ Crowne ⁇ is intermediate to superior in its average growth rate and has good to excellent winter hardiness and will persist in regions north to Kansas, Missouri and Illinois.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

An asexually reproduced variety of perennial Zoysia japonica with a unique combination of characters including white stigmas, anthers which are 5.0 RP +E,fra 3/4+EE , and a distinct DNA fingerprint.

Description

BRIEF SUMMARY OF THE INVENTION
The present invention relates to a new and distinct asexually reproduced variety of perennial zoysiagrass (Zoysia japonica).
BACKGROUND OF THE INVENTION
This invention relates to a new and distinct perennial zoysiagrass cultivar identified as `Crowne` zoysiagrass (herein referred to as `Crowne`), that was tested as DALZ8512. `Crowne`, a Zoysia japonica, is a chance hybrid of the maternal close `Z20` (unpatented), obtained from Beltsville, MD in 1981, with an unknown pollen source from a zoysiagrass germplasm field nursery at TAES--Dallas. `Crowne` has been vegetatively propagated by stolons and rhizomes in Dallas, Tex., and is uniform in growth expression through successive generations.
For purposes of registration under the "International Convention for the Protection of New Varieties of Plants" (generally known by its French acronym as the UPOV Convention) and noting Section 1612 of the Manual of Plant Examination Procedures, the new variety of zoysiagrass of the present invention is named `Crowne`.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a photograph of the leaf blade and ligule of `Crowne`.
FIG. 2 is a DNA fingerprint of `Crowne` in contrast to `Meyer` (unpatented) zoysiagrass.
DETAILED DESCRIPTION OF THE PLANT
`Crowne` was characterized in greenhouse and field conditions. `Crowne` is a unique variety of zoysiagrass developed as described above. `Crowne` was identified as being unique and desirable and was then propagated by cutting of stolons and rhizomes, rooting them in soil, and planting of the rooted material to provide planting stock for studying performance and for comparison of morphological characters after propagation. `Crowne` has been propagated by sod, plugs, sprigs, stolons and rhizomes in greenhouse and field studies by hand and mechanical propagation in Dallas, Tex. All distinguishing and defining characteristics are reliably reproduced in stable and firmly fixed form by such means through succeeding generations. Seed reproduction with self-fertility is not common in the Zoysia sp. No seedling establishment from `Crowne` has been noticed in either greenhouse or field studies.
`Crowne` spreads by stolons and rhizomes. It has an intermediate to rapid establishment rate and has low water use requirements. When sprig (stolons) or plug plantings are made in early May, the area should achieve 50-60% average in Dallas, Tex. in 90-120 days, with complete coverage in 190-210 days, in comparison to `Meyer` zoysiagrass which will require 360-400 days for full coverage. The stolons of `Crowne` have a mean internode length of 17.7 mm between the second and third nodes, with a mean internode width of 0.9 mm and node diameter of 1.1; internode length between the fourth to fifth internode was 12.0 mm with a stolon width of 1.8 mm (Tables 1,2). This creates a proportionally short-thick internode unique to `Crowne`. The stolons of `Crowne` root adventitiously at the nodes. Color notations of plant tissues were based on the Munsell Color Charts for Plant Tissues, Munsell Color, Baltimore, Md., 1977. Light quality, photoperiod, and general growth of the plants affect color notations. The internode stolon color of `Crowne` stolons exposed to full sun is 10R 3/2.
Leaf blades of `Crowne` are rolled in the bud and are flat and stiff. The leaf blade length of `Crowne` is from 72.7 to 126.6 mm and 2.96 to 3.23 mm in width (Tables 3,4). The leaf sheath of `Crowne` is distinct from `Meyer` (Table 5). The hairs on the abaxial leaf surface of `Crowne` are sparse in number and are approximately 0.9 mm long. Measured under greenhouse conditions in January 1996, the genetic, adaxial leaf color of `Crowne` is 2.5G 4/4 with `El Toro` (U.S. Plant Pat. No. 5,845) having a leaf color of 2.5GY 5/2, and `Meyer` having a color of 2.5G 3/4. The ligule of `Crowne` is a fringe of silky hairs, approximately 2.5 mm in length for the longest hairs.
Thatch development is a function of age of stand, types of soils and other environmental and cultural conditions. Thatch development was determined in 3 year old field plantings comparing eight commercially available cultivars. `Emerald`, `Miyako` (U.S. Plant Pat. No. 10,187) and `Zeon` (unpatented) produced the greatest depth of thatch (26.3 mm); `Crowne`, `Palisades` and `De Anza` produced an intermediate amount of thatch (25, 23 and 23 mm, respectively), and `Cavalier` (U.S. Plant Pat. No. 10,778) and `Meyer` produced the least amount of thatch (20 and 19 mm, respectively).
`Crowne` has 5.0 RP 3/4 colored anthers and white colored stigmas, undistinguished in shade of color. The inflorescence of `Crowne` is a terminal spike-like raceme, with spikelets on short pedicels. `Crowne` has a mean length floral region of 23.1 mm with a mean of 25.3 florets per raceme.
The chromosome number of `Crowne` is 40.
When `Crowne` was compared with 59 other zoysiagrasses for salinity tolerance, it ranked number 33 in performance and was superior to `Meyer` (Table 6). When compared to other commercial varieties for root growth, `Crowne` was not different from `El Toro` or `Meyer` in average root depth, root weight, or clipping weights (Table 7).
`Crowne` was entered in the National Turfgrass Evaluation Program (NTEP). `Crowne` in the National Turfgrass Evaluation Program Zoysiagrass Trial for the years 1992, 1993, and 1994 had mean quality ratings of 5.8, 5.8, and 5.5 for overall quality (Table 8).
`Crowne` shows intermediate resistance to the zoysiagrass mite (Table 9). The varieties `Meyer`, `Belair` (unpatented), and most experimental zoysiagrasses are very susceptible to the mite. This mite has been found in Maryland, Texas, Florida, and all extensive zones of use for zoysiagrasses.
`Crowne` is distinguished from other zoysiagrass by its coarse texture, thick stolons, and intermediate to good stolon and rhizome production. It has moderate to good shade tolerance and low water use needs and is highly competitive under natural environmental conditions typical of north central Texas. `Crowne` is intermediate to superior in its average growth rate and has good to excellent winter hardiness and will persist in regions north to Kansas, Missouri and Illinois.
              TABLE 1                                                     
______________________________________                                    
Internode length as measured between the second and third nodes,          
internode diameter of the third internode, and node diameter of the       
third                                                                     
node measured on zoysiagrass plants. Plants were growing in a growth      
chamber with a 14-hr daylength, March 1995.                               
              Internode  Internode Node                                   
              length     diameter  diameter                               
Genotype      mm         mm        mm                                     
______________________________________                                    
`Crowne`      17.7 a*    0.9 abc   1.1 bc                                 
`Cavalier`    18.4 a     1.4 abc   1.4 abc                                
`Meyer`       24.1 a     2.0 a     2.0 a                                  
`Palisades` (United                                                       
              29.9 a     1.0 a     1.7 ab                                 
States Plant Patent                                                       
Application Serial                                                        
Number 09/078,184)                                                        
`El Toro`     39.8 a     1.4 abc   1.4 abc-                               
______________________________________                                    
 *Analysis of variance by General Linear Models, with means followed by th
 same letter not significantly different using Tukey's Studentized Range  
 (HSD), alpha = 0.05. Only selected means presented.                      

              TABLE 2                                                     
______________________________________                                    
Zoysiagrass internode lengths and stolon width measurements from the      
fourth to the fifth nodes taken February 1988 on greenhouse grown         
plants.                                                                   
                  Internode   Stolon                                      
                  length      width                                       
Genotype          mm          mm                                          
______________________________________                                    
`Emerald` (unpatented)                                                    
                   6.2 e*     1.0 ghi                                     
`Crowne`          12.0 b-e    1.8 a                                       
`Palisades`       12.3 b-e    1.6 b                                       
`Meyer`           16.5 bc     1.5 b                                       
`Cavalier`        16.7 bc     1.1 efg                                     
______________________________________                                    
 *Means followed by the same letter are not significantly different using 
 the WallerDuncan k ratio test (k ratio = 100). Only selected means       
 presented.                                                               

              TABLE 3                                                     
______________________________________                                    
Leaf blade width and length measured on the third youngest leaf of        
zoysiagrass. Plants were growing in a growth chamber with a 14-hr         
daylength, March 1995.                                                    
              Blade width   Blade length                                  
Genotype      mm            mm                                            
______________________________________                                    
`Cavalier`    1.3 d*        60.7 cd                                       
`Meyer`       2.6 c         74.3 bc                                       
`Crowne`      3.0 abc       72.7 bc                                       
`El Toro`     3.3 abc       68.3 bcd                                      
`Palisades`   3.4 ab        75.6 bc                                       
______________________________________                                    
 *Analysis of variance by General Linear Models, with means followed by th
 same letter not significantly different using Tukey's Studentized Range  
 (HSD), alpha = 0.05. Only selected means presented.                      

              TABLE 4                                                     
______________________________________                                    
Zoysiagrass leaf measurements taken February 1988 on the                  
fourth youngest leaf from greenhouse grown plants.                        
              Blade width   Blade length                                  
Genotype      mm            mm                                            
______________________________________                                    
`Cavalier`    1.8 ij*       58.9 e                                        
`Emerald`     2.0 fgh       30.6 f                                        
`Crowne`      3.2 b         126.6 b                                       
`Palisades`   3.4 b         101.1 c                                       
`Meyer`       3.3 b         82.3 d                                        
______________________________________                                    
 *Means followed by the same letter are not significantly different using 
 the WallerDuncan k ratio test (k ratio = 100). Only selected means       
 presented.                                                               

              TABLE 5                                                     
______________________________________                                    
Zoysiagrass leaf sheath length measurements taken February 1988 on the    
fourth youngest leaf from greenhouse grown plants.                        
                    Sheath length                                         
Genotype            mm                                                    
______________________________________                                    
`Emerald`           16.3 ij                                               
`Cavalier`          28.9 ef                                               
`Meyer`             30.7 cd                                               
`Crowne`            42.9 b*                                               
`Palisades`         51.25 a                                               
______________________________________                                    
 *Means followed by the same letter are not significantly different using 
 the WallerDuncan k ratio test (k ratio = 100). Only selected means       
 presented.                                                               

              TABLE 6*                                                    
______________________________________                                    
Average percent shoot salt injury (average of 20 rating dates) on         
zoysiagrass entries in the 1991 NTEP† Trials.                      
Entry         Source    Species‡                               
                                   % Injury                               
______________________________________                                    
`Diamond`     NTEP 20   matrella   33 ab.English Pound.                   
(U.S. Plant Patent                                                        
No. 10,636)                                                               
`El Toro`     NTEP 13   japonica   38 a-e                                 
`Emerald`     NTEP 10   jap x tenu 42 a-h                                 
`Cavalier`    NTEP 17   matrella   42 a-h                                 
`Crowne`      NTEP      japonica   46 e-k                                 
`Palisades`   NTEP      japonica   46 e-k                                 
`Belair`      NTEP 11   japonica   50 f-k                                 
`Meyer`       NTEP 09   japonica   58 lmn                                 
`Korean Common`                                                           
              NTEP 07   japonica   76 pq                                  
(unpatented)                                                              
______________________________________                                    
 *Selected data set; complete data set includes 59 varieties and cultivars
 †NTEP = National Turfgrass Evaluation Program.                    
 ‡Species identity.                                            
 .English Pound.Means followed by the same letter are not significantly   
 different, based on the WallerDuncan kratio ttest (kratio = 100)
In Marcum, K. B., M. C. Engelke, S. J. Morton and C. Dayton. 1994. Salinity tolerances of selected bermudagrass and zoysiagrass genotypes. TX Turfgrass Res.--1993, Consolidated Prog. Rep. PR 5140: 105-107.
              TABLE 7                                                     
______________________________________                                    
Average mean root depth of zoysiagrass grown in flexible tubes in         
greenhouse studies, Dallas, TX.                                           
         Average Mean  Total Root                                         
                                Clippinge                                 
         Root Depth    Weight   Weights                                   
Variety  mm            mm       mm                                        
______________________________________                                    
`Cavalier`                                                                
         255           278      243                                       
`Belair` 296           330      286                                       
`Palisades`                                                               
         318           457      452                                       
`Emerald`                                                                 
         330           461      241                                       
`Meyer`  333           411      466                                       
`Crowne` 355           497      406                                       
`El Toro`                                                                 
         356           473      391                                       
MSD       79           161      267                                       
______________________________________                                    
 *MSD = minimum significant difference for comparison of means within     
 columns based on the WallerDuncan kratio test where k = 100.
In Marcum, K.B., M.C. Engelke, S.J. Morton, and R.H. White. 1995. Rooting characteristics and associated drought resistance of zoysiagrasses. Agron.
J. 87:534-538.
              TABLE 8                                                     
______________________________________                                    
Mean Turfgrass quality ratings of vegetative zoysiagrass cultivars grown  
in the National Turfgrass Evaluation Program at 23 locations in the US    
for                                                                       
1992, 1993, 1994, and 1995.                                               
                                       Overall                            
           1992     1993               4-Yr Avg.                          
Variety    mm       mm      1994  1995 mm                                 
______________________________________                                    
`Cavalier` 6.0      6.2     5.9   6.0  5.9                                
TC 2033    5.8      6.1     6.1   6.0  5.9                                
`Sunburst` 5.8      5.9     5.8   5.9  5.8                                
(unpatented)                                                              
TC 5018    5.8      5.8     5.9   5.7  5.8                                
`Emerald`  5.7      6.2     6.0   5.7  5.8                                
`Omni` (unpatented)                                                       
           5.6      6.1     6.1   6.0  5.7                                
QT 2004    5.6      6.0     5.9   5.6  5.6                                
DALZ8508   5.6      6.1     5.7   5.6  5.6                                
`Palisades`                                                               
           5.8      5.8     5.5   5.4  5.6                                
DALZ9006   5.6      6.0     5.6   5.5  5.6                                
`Crowne`   5.8      5.8     5.5   5.4  5.6                                
`El Toro`  5.8      5.6     5.3   5.4  5.5                                
CD 259-13  5.3      5.5     5.7   5.5  5.4                                
`Meyer`    5.3      5.7     5.8   5.5  5.4                                
QT 2047    5.4      5.4     5.3   5.2  5.3                                
`Belair`   5.0      5.6     5.6   5.0  5.2                                
DALZ8516   4.7      5.4     5.0   5.0  4.9                                
`Diamond`  4.4      5.0     4.6   4.4  4.4                                
DALZ8501   4.9      4.3     4.0   4.0  4.3                                
DALZ8701   4.2      4.1     3.7   3.6  3.8                                
LSD VALUE  0.2      0.2     0.2   0.2  0.2                                
______________________________________                                    
 To determine statistical differences among entries, subtract one entry's 
 mean from another entry's mean. Statistical difference occurs when this  
 value is larger than the corresponding LSD value (LSD 0.05).
In National Zoysiagrass Test--1991. Final Report 1992-95, NTEP No. 96-15; (Table 4); United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, Md. 20705.
              TABLE 9                                                     
______________________________________                                    
Mean number of mite-damaged leaves per zoysiagrass plant (n = 18).        
        Leaf Texture                 Combined                             
Cultivar                                                                  
        Class      3April92  24April92                                    
                                     Dates                                
______________________________________                                    
DALZ9006                                                                  
        31         0.2a*     0.2 a   0.2 a                                
DALZ8508                                                                  
        2          0.7 b     0.6 ab  0.6 b                                
DALZ8516                                                                  
        2          1.8 d     0.3 ab  1.0 b                                
`Emerald`                                                                 
        3          1.3 bc    0.9 b   1.1 b                                
DALZ8501                                                                  
        1          1.4 cd    2.6 c   2.0 c                                
`Crowne`                                                                  
        4          5.4 ef    2.3 c   3.9 d                                
`El Toro`                                                                 
        4          5.6 ef    2.8 c   4.2 d                                
TC2033  2          4.4 e     4.3 d   4.4 d                                
`Palisades`                                                               
        4          7.5 fg    4.0 cd  5.8 e                                
CD2031  2          7.6 gh    7.9 e   7.8 f                                
`Diamond`                                                                 
        1          7.8 gh    9.4 ef  8.6 g                                
DALZ8701                                                                  
        1          9.3 h     9.4 ef  9.4 g                                
`Cavalier`                                                                
        3          9.1 h     9.8 ef  9.5 g                                
`Meyer` 2          9.9 h     9.9 ef  9.9 g                                
`Belair`                                                                  
        2          10.0 h    9.9 f   9.9 g                                
JZ-1    4          10.0 h    9.9 f   10.0 g                               
______________________________________                                    
 *Data transformed using log (X + 0.5) for analysis. Means in a column    
 followed by the same letter are not significantly different by           
 WallerDuncan kratio test (k = 100) (P = 0.05).                           
 1 Textural class of zoysiagrass where 1 = short, narrow leaves; 2 = short
 wide leaves; 3 = long, narrow leave; and 4 = long, wide leaves.
Reinert, J.A., M.C. Engelke, and S.J. Morton. 1993. Zoysiagrass resistance to the zoysiagrass mite, Eriphyes zoysiae (Acari: Eriophyidae). In R.N. Carrow, N.E. Christians, and R.C. Sherman (Eds.). International Turfgrass Society Research Journal 7. Intertec Publishing Corp., Overland Park, Kans. pp. 349-352.
DETAILED SUMMARY OF DNA FINGERPRINT ANALYSIS
Drs. Bassam and Caetano-Anolles of the University of Tennessee performed the DNA analysis. The zoysiagrass amplification profiles were obtained using primer of sequence 5'-GCCCGCCC-3', and are compared to the standard `Meyer`. Complex banding patterns and amplification fragment length polymorphism's were obtained in all cases. Results indicate bands fall into two categories, those that are common to the species, and those that in combination are characteristic of the cultivar (some identified by dots).

Claims (1)

I claim:
1. A new and distinct cultivar of Zoysia japonica plant as herein shown and described.
US09/076,973 1998-05-13 1998-05-13 Zoysiagrass plant named `Crowne` Expired - Lifetime USPP11570P (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US09/076,973 USPP11570P (en) 1998-05-13 1998-05-13 Zoysiagrass plant named `Crowne`
AU41869/99A AU4186999A (en) 1998-05-13 1999-05-12 Crowne zoysiagrass plant
PCT/US1999/010550 WO1999057963A2 (en) 1998-05-13 1999-05-12 Crowne zoysiagrass plant

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP13166P2 (en) 1998-04-30 2002-11-05 Bladerunner Farms Zoysiagrass plant named ‘Zeon’
USPP13178P2 (en) 1998-04-14 2002-11-05 Bladerunner Farms Zoysiagrass plant named ‘JaMur’
USPP25203P3 (en) * 2013-04-16 2014-12-30 David Doguet Zoysiagrass plant named ‘L1F’
USPP35643P2 (en) 2023-02-01 2024-02-06 David Doguet Zoysiagrass plant named ‘L1FS’

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USPP8553P (en) * 1991-08-01 1994-01-18 Quality Turfgrass Zoysia grass plant `Z-3`

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USPP8553P (en) * 1991-08-01 1994-01-18 Quality Turfgrass Zoysia grass plant `Z-3`

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP13178P2 (en) 1998-04-14 2002-11-05 Bladerunner Farms Zoysiagrass plant named ‘JaMur’
USPP13166P2 (en) 1998-04-30 2002-11-05 Bladerunner Farms Zoysiagrass plant named ‘Zeon’
USPP25203P3 (en) * 2013-04-16 2014-12-30 David Doguet Zoysiagrass plant named ‘L1F’
USPP35643P2 (en) 2023-02-01 2024-02-06 David Doguet Zoysiagrass plant named ‘L1FS’

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WO1999057963A2 (en) 1999-11-18

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