US8062884B2 - Handling kit for analyzing a liquid sample by nucleic acid amplification - Google Patents
Handling kit for analyzing a liquid sample by nucleic acid amplification Download PDFInfo
- Publication number
- US8062884B2 US8062884B2 US12/373,518 US37351807A US8062884B2 US 8062884 B2 US8062884 B2 US 8062884B2 US 37351807 A US37351807 A US 37351807A US 8062884 B2 US8062884 B2 US 8062884B2
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- United States
- Prior art keywords
- sample
- preparation chamber
- transfer tip
- sample preparation
- sample transfer
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Definitions
- the invention relates to a single use handling kit for analyzing a liquid sample, especially by nucleic acid amplification, comprising a disposable sample holding and processing device for being used in an apparatus for is analyzing a liquid sample, especially by nucleic acid amplification, and a sample transfer tip for transferring liquid into the disposable device, the disposable device having a sample preparation chamber which has an outlet and insertion opening which is adapted to receive the sample transfer tip.
- a processing device for nucleic acid amplification is disclosed in U.S. Pat. No. 6,551,841 B1.
- the known device consists of a substrate of silicon or a polymeric material in which channels and chambers are formed.
- the substrate is covered by a cover made of glass or plastic which seals the channels and chambers between the substrate and the cover.
- US 2004/0141880 A1 discloses transfer of liquids to a disposable device with a tip forming an air tight seal.
- the tip contacts only with its front end an inlet port of the disposable device and is successively used for the transfer of several liquids to the device via the same inlet port. This embodiment does not avoid the risk of contamination of the environment and of primary vessels of the liquids transferred.
- a disposable handling kit which facilitates transferring a sample from a primary tube (e.g. sample storage or collection tube) into the processing device and facilitates a safe and contamination free execution of the analysis.
- a primary tube e.g. sample storage or collection tube
- a handling kit meeting these needs is provided according to the invention in that the insertion opening of the sample preparation chamber and the sample transfer tip being dimensioned in such a way that inserting the sample transfer tip into an insertion position in the sample preparation chamber causes a tight seal between an outer wall of the sample transfer tip and an inner wall of the sample preparation chamber, the disposable device also having a vent, in which preferably a filter material is placed, for venting the sample preparation chamber.
- a tight seal between the sample transfer tip and the wall of the sample preparation chamber prevents contamination of the sample and facilitates transferring liquid into the disposable sample holding and processing device.
- the tight seal is distanced from the end of the sample transfer tip which is introduced into the sample preparation chamber by a distance which is at least 300%, preferably at least 50%, especially at least 75%, of the total length of the sample transfer tip.
- the sample transfer tip reaches with the major part of its length into the device, i.e. into the sample preparation chamber, which results in a better and more precise positioning of the sample transfer tip as tilting of the sample transfer tip is reduced.
- a handling kit according to the invention is therefore readily suited for use with automated gripping devices which allow for fast processing and analyzing of sample liquid in an apparatus for analyzing sample by nucleic acid amplification.
- the sample to be analyzed by the handling kit may be a body fluid, e.g. plasma, serum, urine, or any liquid gained by processing, mixing or other treatment of a body liquid.
- body fluid e.g. plasma, serum, urine, or any liquid gained by processing, mixing or other treatment of a body liquid.
- Other possibilities of samples include suspensions of biological material or any liquid containing an analyte.
- FIG. 1 shows an exploded view of an embodiment of a handling kit according to the invention comprising a disposable handling and processing device and a sample transfer tip;
- FIG. 2 shows a perspective view of the body of the disposable handling and processing device shown in FIG. 1 ;
- FIG. 3 shows another perspective view of the device body shown in FIG. 1 ;
- FIG. 4 shows a schematic sketch of the handling kit shown in FIG. 1 ;
- FIG. 5 shows a back view of the device body and inserted tip shown in FIG. 1 ;
- FIG. 6 shows a cross-section view of the FIG. 5 along the line CC
- FIG. 7 shows a cross-section view of FIG. 4 along the line AA.
- FIG. 8 shows a detail of another embodiment in a cross-section view corresponding to FIG. 7 .
- FIG. 1 shows an exploded view of a handling kit 100 comprising a disposable handling and processing device 1 and a sample transfer tip 12 .
- FIGS. 2 and 3 show the body 2 of the disposable sample holding and processing device 1 , which is designed for being used in an apparatus for analyzing a liquid sample by nucleic acid amplification, especially by polymerase chain reaction technique, and therefore is dimensioned for insertion into such an apparatus.
- the device 1 comprises a device body 2 having a structured surface 3 , which comprises grooves and depressions for channels and chambers, and a sealing cover 4 which covers the structured surface 3 thereby forming a wall of an amplification chamber 5 which is designed and intended for performing nucleic acid amplification and of an inlet channel 6 connected to the amplification chamber 5 .
- the device 1 also comprises a binding chamber 7 containing a solid phase adsorber 8 , preferably a glass fiber fleece, for binding nucleic acids contained in the sample liquid.
- the device 1 also comprises a sample preparation chamber 10 with an insertion opening 16 adapted to receive the sample transfer tip 12 .
- the sample preparation chamber 10 has an outlet 9 which is connected via a channel 13 to the binding chamber 7 .
- the sample preparation chamber 10 has a volume of 50 ⁇ l to 20 ml, especially in the range of 200 ⁇ l to 10 ml, and is typically used for lysis of the sample material or, more generally, for a preparation step of the sample.
- the various chambers 5 , 7 , 10 are connected by channels 13 with each other and/or to fluid interface ports 14 , 14 ′.
- the binding chamber 7 has a volume of 5 ⁇ l to 500 ⁇ l, especially 10 ⁇ l to 100 ⁇ l.
- the amplification chamber 5 has a volume of 10 ⁇ l to 100 ⁇ l and is preferably at least as large as the volume of the binding chamber 7 .
- the depth of the amplification chamber 5 , the binding chamber 7 , the channels 6 and 13 measured perpendicular to the sealing cover 4 is in the range of 50 ⁇ m to 2 mm, preferably 100 ⁇ m to 1 mm.
- the channels 6 , 13 have a cross-section area of 0.01 mm 2 to 2 mm 2 , especially 0.04 mm 2 to 0.5 mm 2 .
- FIG. 4 shows a schematic sketch of the function of the handling kit 100 comprising the device 1 and the sample transfer tip 12 .
- the sealing area 43 of the tip and the sealing area 46 of the inner wall of chamber 10 form a tight seal.
- Reagents e.g. for lysis
- a vent 31 which is closed by a filter 32 is also connected to the sample preparation chamber 10 .
- the chamber 10 has an outlet 9 which leads to a fluidic system 23 which comprises the chamber 5 and 7 shown in FIGS. 1 to 3 .
- Fluid control areas 21 and 22 can be used to close channels and thereby control the flow of gases or liquids.
- the fluid control areas may, for example, be closed by heat or pressure applied by an apparatus in which the handling kit 100 is used to analyze a sample.
- the device body 2 comprises a sheet 20 made of a plastic material on which the structured surface 3 forming the channels 6 , 13 and chambers 5 , 7 , 10 is arranged.
- the device body 2 is manufactured by injection molding.
- Suitable plastic materials which are inert with respect to the sample liquid and to reagents, are for example polypropylene, polyethylene, polystyrene, polycarbonate and polymethylmethacrylate.
- a thermo-plastic material is used, especially polypropylene.
- the structured surface 3 of the device body 2 is overlaid by the flat sealing cover 4 thereby forming a wall of the chambers 5 , 7 , 10 and channels 6 , 13 of the device 1 and sealing them tight.
- the sealing cover 4 is a thin sheet material, for example a plastic foil, which touches the device body 2 in sealing areas 38 .
- the sealing cover 4 comprises more than one layer. In the example shown, it comprises a first layer (preferably touching the device body 2 ) made of a material which is inert with respect to the sample liquid and a second layer (wherein preferably the first layer is placed between the device body 2 and the second layer) which is made of a metal, preferably aluminum.
- the second layer is preferably thicker than the first layer.
- the second layer provides an efficient way for transporting heat to the sample liquid or away from it.
- the sealing cover 4 can be connected to a heating or cooling area of an analysis apparatus.
- the thickness of the sealing cover 4 is as small as possible while still ensuring sufficient mechanical strength for reliably sealing the various chambers 5 , 7 , 10 of the device 1 .
- a low thermal capacity, a high heat transfer conductivity and high heat transfer rate are advantageous as they enable faster heating and cooling of the device 1 , respectively of fluids therein.
- the thickness of the sealing cover 4 should not exceed 1 mm, preferably be below 500 ⁇ m. In order to ensure sufficient mechanical strength for a reliable sealing of the chambers 5 , 7 , 10 and of the channels 6 , 13 the thickness should be at least 50 ⁇ m. Especially advantageous is a thickness of 50 ⁇ m to 350 ⁇ m, especially of 60 ⁇ m to 200 ⁇ m.
- Aluminum is particularly well suited as material for the second layer of the sealing cover 4 as it has a very low thermal capacity. Of course, other materials can also be used.
- the thickness of the second layer is preferably 20 ⁇ m to 400 ⁇ m, especially 20 ⁇ m to 200 ⁇ m.
- the function of the first layer is mainly to prevent contact between sample liquid and the second layer it is advantageous to provide the first layer with a thickness as small as possible while still ensuring a continuous layer.
- the thickness of the first layer should therefore be less than 300 ⁇ m, preferably less than 200 ⁇ m, especially less than 100 ⁇ m. Particularly preferred is a thickness of the first layer of 0.1 ⁇ m to 80 ⁇ m.
- the sealing cover 4 is a composite foil comprising the first layer and the second layer.
- the first layer can be laminated to the second layer or sprayed, painted or, for example, vapor deposited on the second layer. More layers can be added to the sealing cover 4 , for example a coat of paint to protect the second layer.
- the overall heat transfer conductivity of the sealing cover 4 is at least 200 ⁇ 2 K ⁇ 1 , preferably at least 2000 ⁇ 2 K ⁇ 1 .
- the sealing cover 4 can be fixed to the device body 2 by means of suitable bonding procedures, e.g. by thermal sealing or by use of an adhesive, e.g. a polyurethane or polymethylmethacrylate adhesive.
- the sealing cover 4 is bonded using thermal bonding or welded, for example by ultrasonic welding or laser welding, to the device body 2 .
- Welding is most feasible if the first layer of the sealing cover 4 consists of the same material as the device body 2 , e.g. polypropylene.
- the sealing cover 4 and the device body 2 have positioning holes (not shown) which are used during manufacturing for precise positioning of the sealing cover 4 on the structured surface 3 .
- the device 1 has fluid interface ports 14 , 14 ′ which are connected to the channels 6 , 13 or chambers 5 , 7 , 10 of the device 1 .
- the fluid interface ports 14 are arranged on a small area side which adjoins both to a large area front, on which the sealing cover 4 is arranged, and a large area back of the device 1 .
- the interface ports 14 , 14 ′ comprise a cylindrical recess for a septum 29 .
- FIG. 3 shows the fluid interface ports 14 are closed by septa 29 to prevent contamination of the device 1 .
- the septa 29 are made of a suitable elastomere which can be pierced by a hollow needle, syringe or a similar device to deliver reagents or process gases into the device 1 .
- the elastomere used for the septa 29 has a shore hardness in the range of 20 to 80 Shore A, preferably in the range of 30 to 60 Shore A.
- the insertion opening of the sample preparation chamber 10 is also arranged on that small area side. This arrangement enables processing of the device 1 in a vertical position in an analysis apparatus.
- the fluid interface port 14 ′ is arranged on the same side as the inlet ports 14 or on a different small area side which also adjoins both to the large area front and the large area back of the device 1 .
- the fluid interface port 14 ′ is connected directly to the amplification chamber 5 and can be used as an outlet port for removing gas and/or liquid from the device 1 .
- the outlet interface port 14 ′ is arranged on a small area side opposite to the small area side on which the inlet fluid interface ports 14 are arranged.
- the device 1 has a vent 31 connected to the sample preparation chamber 10 via an insertion opening.
- the vent 31 is provided with means 19 , 32 for blocking passage of liquid or solid particles to prevent contamination of a sample with dust, aerosols or the like and to prevent contamination of ambient with potentially dangerous sample material.
- These means comprise a filter material 32 , preferably a porous material, which is placed in the vent 31 .
- the means may also comprise a tortuous section 19 a channel 13 which causes liquid or solid particles to adhere to curving channel walls so that such particles are thereby taken out of a gas flow.
- the tortuous section 19 is the more effective the more curves it comprises and the smaller their curving radii are. In the example shown the tortuous section 19 comprises only a single curve which suffices to provide a filtering effect.
- the means 19 , 32 for blocking passage of liquid or solid particles allow a gas exchange of the preparation chamber 10 with a surrounding atmosphere, usually air.
- a porous plastic material 32 is used to close the vent 31 which is placed on the back of the device 1 .
- the described disposable sample holding and processing device 1 is part of the handling kit 100 which also comprises the sample transfer tip 12 for transferring liquid into the disposable device.
- the handling kit 100 is shown in a back view in FIG. 5 and in a cross-section view along line CC of FIG. 5 in FIG. 6 .
- the sample transfer tip 12 is made of the same polymeric material as the body 2 of the disposable device 1 , i.e. of polypropylene, although the sample transfer tip 12 could in principle also be made of a different material like glass.
- the disposable device 1 has a sample preparation chamber 10 with an insertion opening adapted to receive the sample transfer tip 12 .
- the insertion opening and the sample transfer tip 12 are dimensioned in such a way that inserting the sample transfer tip 12 into the sample preparation chamber 10 causes a tight seal between an outer wall 40 of the sample transfer tip 12 and an inner wall 41 of the sample preparation chamber 10
- the inner wall 41 of the sample preparation chamber 10 has a sealing area 46 which engages a sealing area 43 of the outer wall 40 of the sample transfer tip 12 to form the tight seal.
- the inner wall 41 and the sealing 43 of the sample preparation chamber 10 and the outer wall 40 of the sample transfer tip 12 , between which the tight seal is formed, are circular. When the seal is in place the inner wall 41 of the sample preparation chamber 10 presses against the sample transfer tip 12 .
- the outer diameter of the sample transfer tip 12 is typically in the range of 5 mm to 20 mm. In this way the sample transfer tip 12 can be used to pick up a sample from a blood collection tube or similar device where a sample may be stored.
- the sample transfer tip 12 has an end 15 for insertion into an insertion opening of the sample preparation chamber 10 .
- the end 15 of the sample transfer tip 12 is distanced from the insertion opening 16 ( FIG. 1 ), i.e. its rim 11 , by at least 1 cm, preferably at least 3 cm, especially at least 5 cm.
- the distance between the end 15 of the sample transfer tip 12 an the sealing area 43 is larger than the immersion depth with which the sample transfer tip 12 is immersed in a sample liquid during a sample collection process when sample is taken from a sample reservoir, e.g. by aspiration.
- the tip 12 is friction locked in the device 1 by applying a suitable pushing force which pushes the tip 12 into its insertion position.
- This force is typically in the range of 2 N to 50 N, preferably between 5 N to 30 N.
- the friction lock between the sample transfer tip 12 in the insertion position and the disposable device creates a locking force of at least 2 N, preferably at least 5 N.
- a force of at least 2 N, preferably at least 5 N would be necessary to pull the tip out of its insertion position.
- the sealing area 43 of the sample transfer tip 12 is provided as a frustum shaped section of the tip 12 , but may easily be provided by different means.
- the sample transfer tip 12 contains a plug 50 which is shown in FIG. 1 and made of a filter material, preferably a porous material. Fibrous materials, adsorptive materials, size exclusion materials and/or membranes may also be used.
- the plug 50 is made of a porous plastic material. The plug 50 prevents contamination but is sufficiently permeable for air to communicate pressure and therefore allow sample aspiration and dosing as well as sip and spit mixing of sample liquid with reagents in the sample preparation chamber 10 .
- the plug 50 filters aerosols from air which the device exchanges with a surrounding atmosphere.
- FIG. 7 shows a cross-section view along line AA of FIG. 5 .
- the sheet 20 carries at least one rib 34 , 35 , 36 for increasing the stiffness of the device body 2 .
- the ribs 34 , 35 , 36 and the sheet 20 are manufactured as a single piece.
- ribs 34 , 35 , 36 are arranged both on the front side (i.e. on the structured surface 3 facing to the cover 4 ) of the sheet 20 and on the back side (the opposite side of the sheet 20 facing away of the sheet 20 ) of the sheet 20 for increased stiffness.
- a useful stiffening effect can also be achieved with ribs on either the front or back side of the sheet 20 only, or even by a single rib.
- At least one rib 35 , 36 is arranged on the structured surface 3 such that at least one wall of a channel 6 , 13 is formed by the rib.
- opposing walls of the channel 6 or correspondingly of another channel 13
- neighboring walls forming the channel 6 in between that walls are formed by two corresponding ribs 35 , 36 running parallel to each other.
- the channel bottom 37 is elevated with respect to the surface of the sheet 20 adjacent to the ribs 35 , 36 , which form opposing walls of the channel 6 , as shown in FIG. 8 .
- ribs 35 , 36 or a raised section form sidewalls of the binding chamber 7 and the amplification chamber 5 .
- the sealing cover 4 is fixed to the ribs 35 , 36 on the front side of the sheet 20 and therefore touches the device body 2 only with a fraction of its surface area, which eases creation of a tight seal between the disposable body 2 and the sealing cover 4 and reduces bending of the device 1 .
- ribs 35 and 36 have flat tops which are connected to the sealing cover 4 .
- pockets of air 45 exist between the sheet 20 and the cover 4 .
- This provides for thermal insulation between the device body 2 and the sealing cover 4 .
- an improved thermal connection between the sealing cover 4 and sample liquid is achieved as the sealing cover 4 forms a wall to the various channels 6 , 13 and chambers 5 , 7 , 10 of the device 1 .
- the rib 34 or ribs on the back side of the sheet 20 are aligned with the inlet channel 6 or one or several other channels 13 on the front of the sheet 20 or with a chamber wall, no matter whether that channel 6 , 13 or wall of a chamber 5 , 7 , 10 is straight or curved.
- the at least one rib 34 is parallel to a straight channel 6 , 13 and/or to a straight portion of a channel 6 , 13 and/or to a straight chamber wall. It is especially advantageous to arrange at least one the rib 34 or ribs on the back side of the sheet 20 , i.e. on the side not covered by the sealing cover 4 .
- the at least one rib 34 is opposite of channels 6 , 13 as shown in FIGS. 7 and 8 and/or the sealing area 38 in which the cover sheet 4 is connected to the device body 2 . For additional stiffening further ribs may be added, especially on the back side of the sheet 20 .
- the sheet 20 has a thickness of 0.2 mm to 4 mm, especially 0.3 mm to 2 mm, preferably 0.5 mm to 1.5 mm, especially preferred of 0.8 mm to 1.0 mm.
- the ribs 34 on the back side of the sheet 20 have typically at half height a width which is 50% to 150% of the thickness of the sheet 20 .
- the ribs 34 rise above the surface of the sheet 20 to a height which is 60% to 200%, preferably 80% to 150% of the thickness of the sheet 20 .
- Ribs 35 , 36 on the front side of the sheet 20 have a smaller height than ribs 34 on the back side of the sheet 20 , i.e. ribs 35 , 36 on the front side of the sheet 20 have preferably a height of 20% to 120% of the thickness of the sheet 20 .
- ribs 34 on the back side of the sheet 20 and ribs 35 , 36 on its front side are largely due to differences in their function. Whereas ribs 34 serve only to increase the stiffness of the device body 2 , ribs 35 , 36 first and foremost serve to provide walls of one or several channels 6 , 13 and/or to connect the device body 2 to the cover 4 . Although the ribs 35 , 36 are therefore much smaller in height they still provide a welcome stiffening effect.
Abstract
Description
-
- The device should be suitable for performing a rather complex processing, e.g. forming the binding solution for the nucleic acid analysis, with enabling performing standard operation steps like addition of the sample, addition of reagents, incubation, closing of the chamber and pumping out of the chamber.
- The transfer of a sample into the disposable device with a disposable tip should be possible.
- When a sample, in particular a biological sample, is taken from a primary vessel by the tip there is a high risk that the outside of the tip is contaminated, at least to the extent at which the tip was immersed into the primary vessel or into the fluid comprised in the primary vessel. In order to avoid a contamination of the environment the tip should be possibly be immersed into a chamber of the device surrounding the tip at least to the extent at which it was immersed into the primary vessel or into the liquid in the primary vessel. Further, the tip should be kept in that in the chamber of the device after use of the tip upon using the device in order to avoid a contamination of the environment.
- In order to avoid a contamination of the tip before its use for transfer of a sample to the disposable device, e.g. by aerosols or drops, it is preferable when it can be kept for that purpose in the secured position in a chamber of the disposable device.
- In order to avoid spread, transmission and carry over of a contamination from a sample to a reagent container it should be possible to use separate ports for providing sample and for providing reagents to the disposable device.
- In order to avoid a contamination of the environment in mixing steps the chamber should be vented in a controlled manner
- 1 disposable sample holding and processing device
- 2 device body
- 3 structured surface
- 4 sealing cover
- 5 amplification chamber
- 6 inlet channel
- 7 binding chamber
- 8 solid phase adsorber
- 9 outlet of
sample preparation chamber 10 - 10 sample preparation chamber
- 11 rim of
insertion opening 16 of thesample preparation chamber 10 - 12 sample transfer tip
- 13 channels
- 14 interface port
- 14′ interface port
- 15 end of the
sample transfer tip 12 - 16 insertion opening of the sample preparation chamber
- 19 tortuous section of
channel 13 - 20 sheet
- 21 fluid control area
- 22 fluid control area
- 23 fluidic
system comprising channels chambers - 29 septa
- 31 vent
- 32 filter material
- 34 rib (on back side of sheet 20)
- 35 rib (on front side of sheet 20)
- 36 rib (on front side of sheet 20)
- 37 channel bottom
- 40 outer wall of the
sample transfer tip 12 - 41 inner wall of the
sample preparation chamber 10 - 43 sealing area of tip
- 45 air pocket
- 46 sealing area of chamber
- 50 plug
- 100 handling kit
Claims (15)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06014684 | 2006-07-14 | ||
EP06014684.2 | 2006-07-14 | ||
EP06014684A EP1878498A1 (en) | 2006-07-14 | 2006-07-14 | Handling kit for analyzing a liquid sample by nucleic acid ampification |
PCT/EP2007/005954 WO2008006503A1 (en) | 2006-07-14 | 2007-07-05 | Handling kit for analyzing a liquid sample by nucleic acid amplification |
Publications (2)
Publication Number | Publication Date |
---|---|
US20090317899A1 US20090317899A1 (en) | 2009-12-24 |
US8062884B2 true US8062884B2 (en) | 2011-11-22 |
Family
ID=37531848
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/373,518 Expired - Fee Related US8062884B2 (en) | 2006-07-14 | 2007-07-05 | Handling kit for analyzing a liquid sample by nucleic acid amplification |
Country Status (4)
Country | Link |
---|---|
US (1) | US8062884B2 (en) |
EP (2) | EP1878498A1 (en) |
AT (1) | ATE548116T1 (en) |
WO (1) | WO2008006503A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9707556B2 (en) | 2007-08-17 | 2017-07-18 | Diagnostics For The Real World, Ltd. | Device, system and method for processing a sample |
US9839909B2 (en) | 2006-07-28 | 2017-12-12 | Diagnostics For The Real World, Ltd. | Device, system and method for processing a sample |
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DE102008042581B4 (en) * | 2008-10-02 | 2012-02-02 | INSTITUT FüR MIKROTECHNIK MAINZ GMBH | Microfluidic extraction and reaction device |
US20140037516A1 (en) | 2011-03-15 | 2014-02-06 | Carclo Technical Plastics Limited | Surface preparation |
US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
CN105008914B (en) * | 2013-02-28 | 2018-06-15 | 安捷伦科技有限公司 | For the integrated fluid connection of the flat structure of sample separating apparatus |
CN111257548B (en) * | 2020-02-28 | 2022-01-14 | 广州万孚生物技术股份有限公司 | In vitro diagnosis analyzer and reagent card |
DE102022200662A1 (en) * | 2022-01-21 | 2023-07-27 | Robert Bosch Gesellschaft mit beschränkter Haftung | Microfluidic device and method of using a microfluidic device |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0264704A2 (en) | 1986-10-20 | 1988-04-27 | Eppendorf Gerätebau Netheler + Hinz GmbH | Pipetting device provided with a conical adapter for a pipette tip, and pipette tip for such a device |
US20040096358A1 (en) | 2002-11-14 | 2004-05-20 | Gert Blankenstein | Device for the stepwise transport of liquid utilizing capillary forces |
US20040141880A1 (en) | 2002-07-24 | 2004-07-22 | Erich Handler | System and cartridge for processing a biological sample |
US20050148091A1 (en) | 1999-08-11 | 2005-07-07 | Asahi Kasei Kabushiki Kaisha | Analyzing cartridge and liquid feed control device |
EP1643254A2 (en) | 2004-10-04 | 2006-04-05 | Fuji Photo Film Co., Ltd. | Sample supplying method and device |
WO2007005954A1 (en) | 2005-07-01 | 2007-01-11 | Purdue Research Foundation | Integrated thξrmochemical and biocatalytic energy production apparatus and method |
-
2006
- 2006-07-14 EP EP06014684A patent/EP1878498A1/en not_active Withdrawn
-
2007
- 2007-07-05 US US12/373,518 patent/US8062884B2/en not_active Expired - Fee Related
- 2007-07-05 EP EP07765075A patent/EP2040840B1/en not_active Not-in-force
- 2007-07-05 AT AT07765075T patent/ATE548116T1/en active
- 2007-07-05 WO PCT/EP2007/005954 patent/WO2008006503A1/en active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0264704A2 (en) | 1986-10-20 | 1988-04-27 | Eppendorf Gerätebau Netheler + Hinz GmbH | Pipetting device provided with a conical adapter for a pipette tip, and pipette tip for such a device |
US4999164A (en) | 1986-10-20 | 1991-03-12 | Eppendorf-Netheler-Hinz Gmbh | Pipetting device comprising a retaining cone for holding a slip-on pipette tip and pipette tip for such pipetting device |
EP0264704B1 (en) | 1986-10-20 | 1992-03-04 | Eppendorf Gerätebau Netheler + Hinz GmbH | Pipetting device provided with a conical adapter for a pipette tip, and pipette tip for such a device |
US20050148091A1 (en) | 1999-08-11 | 2005-07-07 | Asahi Kasei Kabushiki Kaisha | Analyzing cartridge and liquid feed control device |
US20040141880A1 (en) | 2002-07-24 | 2004-07-22 | Erich Handler | System and cartridge for processing a biological sample |
US20040096358A1 (en) | 2002-11-14 | 2004-05-20 | Gert Blankenstein | Device for the stepwise transport of liquid utilizing capillary forces |
EP1643254A2 (en) | 2004-10-04 | 2006-04-05 | Fuji Photo Film Co., Ltd. | Sample supplying method and device |
WO2007005954A1 (en) | 2005-07-01 | 2007-01-11 | Purdue Research Foundation | Integrated thξrmochemical and biocatalytic energy production apparatus and method |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9839909B2 (en) | 2006-07-28 | 2017-12-12 | Diagnostics For The Real World, Ltd. | Device, system and method for processing a sample |
US10315195B2 (en) | 2006-07-28 | 2019-06-11 | Diagnostics For The Real World, Ltd. | Device, system and method processing a sample |
US9707556B2 (en) | 2007-08-17 | 2017-07-18 | Diagnostics For The Real World, Ltd. | Device, system and method for processing a sample |
US10661271B2 (en) | 2007-08-17 | 2020-05-26 | Diagnostics For The Real World, Ltd. | Device, system and method for processing a sample |
Also Published As
Publication number | Publication date |
---|---|
EP2040840A1 (en) | 2009-04-01 |
ATE548116T1 (en) | 2012-03-15 |
EP2040840B1 (en) | 2012-03-07 |
US20090317899A1 (en) | 2009-12-24 |
WO2008006503A1 (en) | 2008-01-17 |
EP1878498A1 (en) | 2008-01-16 |
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