US4103025A - Novel terpenes - Google Patents
Novel terpenes Download PDFInfo
- Publication number
- US4103025A US4103025A US05/778,458 US77845877A US4103025A US 4103025 A US4103025 A US 4103025A US 77845877 A US77845877 A US 77845877A US 4103025 A US4103025 A US 4103025A
- Authority
- US
- United States
- Prior art keywords
- compound
- cicatrizing
- amount
- accordance
- animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/08—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals directly attached to carbocyclic rings
Definitions
- This invention relates to novel pharmaceutically active compounds, to processes for their production, to pharmaceutical compositions containing the compounds and to plant extracts from which the compounds may be prepared.
- Plants of the genus Terminalia (of the family Combretaceae) are distributed over regions of Africa, Asia, Australia and tropical America. We have now discovered that extracts possessing valuable pharmaceutical properties may be obtained from plants of one particular species of this genus, namely Terminalia sericea.
- Sericic acid and sericoside have been found to possess valuable cicatrigin, and anti-inflammatory properties which render them particularly useful in treating dermatological disorders, for example by being incorporated into cosmetics and in treating stomach ulcers. Sericic acid and sericoside may also be converted into derivatives which have similar valuable pharmacological properties.
- R 1 , R 2 , R 3 and R 4 which may be the same or different, may, for example, be substituted or unsubstituted aliphatic or aromatic mono- or poly-carboxylic acid acyl radicals, particularly such radicals containing up to twelve and preferably up to seven carbon atoms.
- the aliphatic mono- or poly-carboxylic acid acyl radicals may be straight or branched, chained or cyclic and furthermore may be saturated or unsaturated.
- saturated, unsubstituted aliphatic mono-carboxylic acid acyl radicals include acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl and heptanoyl. Particularly preferred are such radicals containing up to four carbon atoms.
- poly-carboxylic acid acyl radicals examples include hemi-maleyl, hemi-fumaryl and hemi-succinyl.
- aromatic carboxylic acid acyl radicals examples include benzoyl and phenylacetyl.
- the or each substituent may, for example, be selected from halogen (i.e. fluorine, chlorine, bromine or iodine), nitro, hydroxy, ether, keto and amino groups.
- the aromatic mono- or polycarboxylic acid acyl radicals may additionally or alternatively be substituted by one or more aliphatic radicals (optionally substituted, for example by one or more of the substituents referred to above) and preferably containing up to six carbon atoms as in methyl, ethyl, propyl, isopropyl, butyl, isobutyl, valeryl and hexyl.
- Particularly preferred acryl radicals for R 1 , R 2 , R 3 and R 4 are acetyl, benzoyl and hemi-succinyl.
- R 1 , R 2 , R 3 and R 4 represents an acyl radical derived from a polycarboxylic acid
- the remaining carboxyl (--COO--) group or groups may be in the form of the free acid (--COOH) or in the form of a derivative, for example an acid addition salt with a pharmaceutically acceptable cation or an ester with, for example, an aliphatic alcohol containing up to seven carbon atoms, such as, for example, methanol, ethanol, propanol, isopropanol, n-butanol, pentanol, hexanol and heptanol.
- R 5 may, for example, be hydrogen or a substituted or unsubstituted straight, branched chain or cyclic aliphatic radical, particularly such radicals containing up to twelve and preferably up to seven carbon atoms.
- examples of such radicals include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl, pentyl, cyclopentyl, hexyl, cyclohexyl and heptyl.
- the or each substitutent may, for example, be selected from halogen (i.e. fluorine, chlorine, bromine or iodine), nitro, hydroxy, ether, keto and amino groups. Any amino groups may be further substituted by one or two aliphatic radicals (which may be the same or different), for example the radicals specified above.
- halogen i.e. fluorine, chlorine, bromine or iodine
- nitro, hydroxy, ether, keto and amino groups Any amino groups may be further substituted by one or two aliphatic radicals (which may be the same or different), for example the radicals specified above.
- radicals represented by R 5 include hydrogen; the alkyl radicals referred to above; aminoalkyl radicals or mono- or dialkylaminoalkyl radicals (--R'--NR"R'") where --R'-- is a straight or branched chain alkylene radical, preferably containing up to seven carbon atoms (for example as in aminomethyl, 1,1- or 1,2-aminoethyl, aminopropyl, aminobutyl, aminopentyl, aminohexyl or aminoheptyl) and R" and R'", which may be the same or different are selected from hydrogen atoms and alkyl groups (for example the alkyl groups specified above); and glycosyl residues, particularly D-glucosyl.
- the invention also includes pharmaceutical compositions comprising as active ingredient a compound of the general formula (II) or (III) or a pharmaceutically acceptable salt thereof in combination with a pharmaceutically acceptable excipient and processes for producing such compositions which comprise admixing the active ingredient with the excipient.
- excipients are starch, lactose, propylene glycol, triethanolamine, water and anti-fermentative agents.
- a process for producing a compound of general formula (II) or (III) which comprises extracting tissue of a plant of the species Terminalia sericea, and in particular the roots or the bark of the roots with an organic solvent, isolating sericic acid and/or sericoside from the extract and if desired converting the isolated sericic acid or sericoside to another compound of general formula (II) or (III).
- the organic solvent may, for example, be an alcohol (preferably a lower alcohol containing up to six carbon atoms and most preferably up to four carbon atoms, such as methanol, ethanol, n-propanol, isopropanol, or one of the butanols), a ketone (preferably a di-lower alkyl ketone wherein the lower alkyl groups contain up to six and preferably up to four carbon atoms as in acetone, methyl ethyl ketone and di-isopropyl ketone) or an ester (preferably an ester formed with a lower alcohol containing up to six carbon atoms, and most preferably up to four carbon atoms, such as methanol, ethanol, n-propanol, isopropanol, the butanols and amyl alcohol and a lower alkanoic acid containing up to four carbon atoms, such as formic, acetic, propionic or butyric acids).
- an alcohol
- Particularly useful solvents are ethanol, acetone and ethyl acetate.
- organic solvent is miscible with water
- mixtures of the solvent and water may be used.
- fatty and resinous substances are removed, for example by evaporating the organic solvent from the extract and contacting the residue so obtained with a liquid hydrocarbon so as to form a partially purified extract.
- the residue is diluted with water or with an alcohol/water mixture before it is contacted with the hydrocarbon.
- the fatty and resinous substances are preferentially dissolved in the hydrocarbon and may therefore be removed by decanting the resulting hydrocarbon solution.
- the isolation of sericic acid and sericoside from the partially purified extract obtained as described above therefore preferably includes a fractionation step in which the extract is fractionated into a fraction containing sericic acid and other non-glucosidated terpenes (Fraction A) and a fraction containing sericoside and other terpenic glucosides by contacting a solution of the partially purified extract in water or a mixture of water and an alcohol (for example one of the alcohols specified above) with a chlorinated hydrocarbon solvent such as, for example, chloroform, methylene chloride or dichloroethane, and separating the aqueous and chlorinated hydrocarbon solutions so obtained.
- Fraction A fraction containing sericic acid and other non-glucosidated terpenes
- a chlorinated hydrocarbon solvent such as, for example, chloroform, methylene chloride or dichloroethane
- the aqueous solution may then be diluted with alcohol to give an alcohol concentration of not less than 60% and treated to remove proteinaceous material by adding neutral or basic lead acetate solution and filtering to remove any precipitate which is formed.
- the filtrate may then be evaporated to form an aqueous concentrate by eliminating the alcoholic solvent and the terpenic glucosides extracted repeatedly from the concentrate with a mixture of butanol and benzene (preferably 3:1 to 4:1),
- Sericic acid and sericoside may be obtained from Fractions A and B respectively by recrystallization or chromatographic separation.
- Sericic acid and sericoside may each be transformed into each of the other compounds of general formulae (II) and (III) by known methods of esterification, hydrolysis, oxidation and salt formation and such methods form a further aspect of the present invention.
- sericic acid may be converted to sericoside by esterification with ⁇ -bromo-(tetra-acetyl)-glucose followed by successive elimination of the acetyl radicals and sericoside may be converted to sericic acid by saponification.
- the aqueous residue remaining after the extraction with dichloroethane is diluted with 500 liters of alcohol and 10 kg. of neutral lead acetate dissolved in 30 liters of alcohol are added. An abundant precipitate is formed and is left to stand overnight to sediment. The precipitate is centrifuged and discarded, and the water-alcohol phase is concentrated in vacuo to about 200 liters.
- the aqueous concentrate is extracted three times with a mixture of t-butanol-benzene in the ratio 3:1 (v/v) using 100 liters each time.
- Fraction A 1 kg. of Fraction A is dissolved in 5 liters of alcohol. The solution is decolorized with carbon, evaporated to 3 liters and left to stand overnight.
- the sericic acid triacetate may be converted to sericic acid, in a state of purity such that its properties are entirely identical with that obtained by crystallization, as described above.
- Fraction A 1 kg. of Fraction A is dissolved in 3 volumes of chloroform and chromatographed on a column containing 15 kg. of silica gel by eluting with a solvent mixture comprising 95:5 chloroform - ethanol.
- the fractions containing the individual pure components are collected and reunited and after crystallization from methanol, 250 g. of pure sericic acid having a m.p. 278° - 82° C. are obtained along with 20 g. of arjunic acid with m.p. 220 ° - 22° C.
- Fraction B 1 kg. of Fraction B is dissolved in 6 liters of alcohol. The solution is decolorized with carbon and evaporated to 3 liters.
- fraction B 1 kg. of fraction B is dissolved in 3 liters of an 8:2 (v/v) chloroform - ethanol mixture and chromatographed on 30 kg. of silica gel by eluting with the said solvent mixture.
- the fractions containing the individual pure products are reunited and concentrated to dryness. After crystallization from methanol there are obtained respectively 320 g. of sericoside having an m.p. of 206 - 208° C. and 210 g. of arjunetine.
- the acetone solution is diluted with 1,500 ml. of water whereupon the product precipitates in amorphous form.
- the product melts at 223° C.
- reaction mixture is reheated to 80° C. for 6 hours, then cooled and diluted with 800 ml. of chloroform.
- the solution is counter-washed with 10% aqueous HCl until the pyridine is eliminated and then concentrated to dryness and dehydrated over Na 2 SO 4 .
- the anti-ulcer activity of sericic acid and sericoside was determined by administering the compounds to rats in which gastric ulcers were induced by Shay's method.
- the compounds were administered at the dose of 200 mg./kg. orally five times, at 42, 30, 25 and 6 hours before tying of the pylorus and immediately after the operation and as shown in Table 1 diminished the ulcer index by 48 and 45 per cent respectively, compared with the controls.
- mice (as determined with intraperitoneal administration) for sericic acid and sericoside was as follows:
- the anti-inflammatory activities of sericoside and sericic acid were determined by measuring the extent to which the oedema caused by sub-plantar administration of carragenin to rats could be inhibited by prior oral and intraperitoneal administration of the substances.
- the cicatrizing activity of sericic acid and sericoside upon experimental wounds was determined on rats in accordance with the method of Morton & Malone (Arch. Int. Pharmacodyn. Ther. 196, 117, 1972).
- the treatment of the wounds was effected with a 10% suspension of the active principles in water containing 2% carboxymethyl cellulose (CMC).
- CMC carboxymethyl cellulose
- Ultra-violet ray erythema in guinea pigs was effected in accordance with the method of Winder et al. (Arch. Int. Pharmacodyn, 106, 261, 1968).
- Sericoside and sericic acid were applied to the depilated skin of male albino guinea pigs (average weight 300 - 400 g) in the form of a 5% gel (using 200 mg. thereof) one hour prior to irradiation.
- the animals were depilated 18 hours prior to the irradiation and irradiated for 3 minutes with a 500 W. mercury vapor lamp at a distance of 18 cms.
- the controls were treated with a similar gel which did not contain the active ingredients, sericoside or sericic acid.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Pharmaceutically active compounds of the formula ##STR1## wherein --OR1, --OR2, --OR3 and --OR4, which may be the same or different, each represents a free or esterified hydroxyl group and --COOR5 represents a free or esterified carboxyl group, are provided together with processes for their production and pharmaceutical compositions containing them. Compounds of formula II and III possess valuable cicatrizing and anti-inflammatory properties.
Description
This is a continuation, of application Ser. No. 541,588, filed Jan. 16, 1975, now abandoned.
This invention relates to novel pharmaceutically active compounds, to processes for their production, to pharmaceutical compositions containing the compounds and to plant extracts from which the compounds may be prepared.
Plants of the genus Terminalia (of the family Combretaceae) are distributed over regions of Africa, Asia, Australia and tropical America. We have now discovered that extracts possessing valuable pharmaceutical properties may be obtained from plants of one particular species of this genus, namely Terminalia sericea.
These extracts have a high content of terpenic compounds and, in addition to the known compounds arjunic acid and arjunetine, they have been found to contain two hitherto unknown compounds which we have named sericic acid and sericoside. These compounds are respectively 2α,3β,19α,24-tetrahydroxy-olean-12-en-28-oic acid and its D-glucopyranoside ester, and may be represented by the formula (I) ##STR2## wherein in the case of sericic acid, R = hydrogen and in the case of sericoside, R = glucose.
Sericic acid and sericoside have been found to possess valuable cicatrigin, and anti-inflammatory properties which render them particularly useful in treating dermatological disorders, for example by being incorporated into cosmetics and in treating stomach ulcers. Sericic acid and sericoside may also be converted into derivatives which have similar valuable pharmacological properties.
Thus in accordance with one aspect of the present invention, there are provided compounds of the general formula (II) or (III) ##STR3## wherein --OR1, --OR2, --OR3 and --OR4 each represents a free or esterified hydroxyl group and --COOR5 represents a free or esterified carboxyl group, and pharmaceutically acceptable salts of such compounds which are capable of salt formation.
R1, R2, R3 and R4, which may be the same or different, may, for example, be substituted or unsubstituted aliphatic or aromatic mono- or poly-carboxylic acid acyl radicals, particularly such radicals containing up to twelve and preferably up to seven carbon atoms.
The aliphatic mono- or poly-carboxylic acid acyl radicals (both substituted and unsubstituted) may be straight or branched, chained or cyclic and furthermore may be saturated or unsaturated. Examples of saturated, unsubstituted aliphatic mono-carboxylic acid acyl radicals include acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl and heptanoyl. Particularly preferred are such radicals containing up to four carbon atoms.
Examples of poly-carboxylic acid acyl radicals include hemi-maleyl, hemi-fumaryl and hemi-succinyl.
Examples of aromatic carboxylic acid acyl radicals include benzoyl and phenylacetyl.
Where such radicals are substituted, the or each substituent may, for example, be selected from halogen (i.e. fluorine, chlorine, bromine or iodine), nitro, hydroxy, ether, keto and amino groups. The aromatic mono- or polycarboxylic acid acyl radicals may additionally or alternatively be substituted by one or more aliphatic radicals (optionally substituted, for example by one or more of the substituents referred to above) and preferably containing up to six carbon atoms as in methyl, ethyl, propyl, isopropyl, butyl, isobutyl, valeryl and hexyl.
Particularly preferred acryl radicals for R1, R2, R3 and R4 are acetyl, benzoyl and hemi-succinyl.
Where any one of R1, R2, R3 and R4 represents an acyl radical derived from a polycarboxylic acid, the remaining carboxyl (--COO--) group or groups may be in the form of the free acid (--COOH) or in the form of a derivative, for example an acid addition salt with a pharmaceutically acceptable cation or an ester with, for example, an aliphatic alcohol containing up to seven carbon atoms, such as, for example, methanol, ethanol, propanol, isopropanol, n-butanol, pentanol, hexanol and heptanol.
R5 may, for example, be hydrogen or a substituted or unsubstituted straight, branched chain or cyclic aliphatic radical, particularly such radicals containing up to twelve and preferably up to seven carbon atoms. Examples of such radicals include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl, pentyl, cyclopentyl, hexyl, cyclohexyl and heptyl.
Where such radicals are substituted, the or each substitutent may, for example, be selected from halogen (i.e. fluorine, chlorine, bromine or iodine), nitro, hydroxy, ether, keto and amino groups. Any amino groups may be further substituted by one or two aliphatic radicals (which may be the same or different), for example the radicals specified above.
Particularly preferred examples of radicals represented by R5 include hydrogen; the alkyl radicals referred to above; aminoalkyl radicals or mono- or dialkylaminoalkyl radicals (--R'--NR"R'") where --R'-- is a straight or branched chain alkylene radical, preferably containing up to seven carbon atoms (for example as in aminomethyl, 1,1- or 1,2-aminoethyl, aminopropyl, aminobutyl, aminopentyl, aminohexyl or aminoheptyl) and R" and R'", which may be the same or different are selected from hydrogen atoms and alkyl groups (for example the alkyl groups specified above); and glycosyl residues, particularly D-glucosyl.
Compounds of general formulae (II) and (III) containing carboxyl groups (for example where R5 = H or where R1, R2, R3 or R4 contains a free carboxyl group) may be converted to pharmaceutically acceptable acid addition salts, for example with metals yielding pharmaceutically acceptable cations, such as, for example, sodium, potassium, calcium, magnesium, aluminum or iron. Also, compounds containing substituted or unsubstituted amino groups may be converted to pharmaceutically acceptable acid addition salts with acids yielding pharmaceutically acceptable cations (e.g. hydrochloric or sulphuric acids).
The invention also includes pharmaceutical compositions comprising as active ingredient a compound of the general formula (II) or (III) or a pharmaceutically acceptable salt thereof in combination with a pharmaceutically acceptable excipient and processes for producing such compositions which comprise admixing the active ingredient with the excipient. Examples of excipients are starch, lactose, propylene glycol, triethanolamine, water and anti-fermentative agents.
According to a further aspect of the present invention there is provided a process for producing a compound of general formula (II) or (III) which comprises extracting tissue of a plant of the species Terminalia sericea, and in particular the roots or the bark of the roots with an organic solvent, isolating sericic acid and/or sericoside from the extract and if desired converting the isolated sericic acid or sericoside to another compound of general formula (II) or (III).
The organic solvent may, for example, be an alcohol (preferably a lower alcohol containing up to six carbon atoms and most preferably up to four carbon atoms, such as methanol, ethanol, n-propanol, isopropanol, or one of the butanols), a ketone (preferably a di-lower alkyl ketone wherein the lower alkyl groups contain up to six and preferably up to four carbon atoms as in acetone, methyl ethyl ketone and di-isopropyl ketone) or an ester (preferably an ester formed with a lower alcohol containing up to six carbon atoms, and most preferably up to four carbon atoms, such as methanol, ethanol, n-propanol, isopropanol, the butanols and amyl alcohol and a lower alkanoic acid containing up to four carbon atoms, such as formic, acetic, propionic or butyric acids).
Particularly useful solvents are ethanol, acetone and ethyl acetate.
Where the organic solvent is miscible with water, mixtures of the solvent and water may be used.
Preferably, prior to isolating sericic acid and/or sericoside from the extract, fatty and resinous substances are removed, for example by evaporating the organic solvent from the extract and contacting the residue so obtained with a liquid hydrocarbon so as to form a partially purified extract. Preferably, the residue is diluted with water or with an alcohol/water mixture before it is contacted with the hydrocarbon. The fatty and resinous substances are preferentially dissolved in the hydrocarbon and may therefore be removed by decanting the resulting hydrocarbon solution.
Sericic acid has been found to be generally more soluble in chlorinated hydrocarbon solvents than in water and sericoside has been found to be generally more soluble in aqueous solvents than chlorinated hydrocarbons. The isolation of sericic acid and sericoside from the partially purified extract obtained as described above therefore preferably includes a fractionation step in which the extract is fractionated into a fraction containing sericic acid and other non-glucosidated terpenes (Fraction A) and a fraction containing sericoside and other terpenic glucosides by contacting a solution of the partially purified extract in water or a mixture of water and an alcohol (for example one of the alcohols specified above) with a chlorinated hydrocarbon solvent such as, for example, chloroform, methylene chloride or dichloroethane, and separating the aqueous and chlorinated hydrocarbon solutions so obtained.
The aqueous solution may then be diluted with alcohol to give an alcohol concentration of not less than 60% and treated to remove proteinaceous material by adding neutral or basic lead acetate solution and filtering to remove any precipitate which is formed. The filtrate may then be evaporated to form an aqueous concentrate by eliminating the alcoholic solvent and the terpenic glucosides extracted repeatedly from the concentrate with a mixture of butanol and benzene (preferably 3:1 to 4:1),
The solution in the butanol/benzene mixture may then be evaporated to small volume and the residue poured into isopropyl ether. The precipitate so obtained contains a major part of the terpenic glucosides (Fraction B).
Sericic acid and sericoside may be obtained from Fractions A and B respectively by recrystallization or chromatographic separation.
It will be appreciated that the sericic acid- and sericoside-containing extracts of Terminalia sericea referred to above and in particular the purified fractions referred to as Fractions A and B constitute further aspects of the present invention.
Sericic acid and sericoside may each be transformed into each of the other compounds of general formulae (II) and (III) by known methods of esterification, hydrolysis, oxidation and salt formation and such methods form a further aspect of the present invention.
Thus, for example, sericic acid may be converted to sericoside by esterification with α-bromo-(tetra-acetyl)-glucose followed by successive elimination of the acetyl radicals and sericoside may be converted to sericic acid by saponification.
The following Examples illustrate the invention:
200 kg. of finely ground roots of plants of the species Terminalia sericea are extracted under weak reflux four times using 600 liters of aqueous alcohol each time. The reunited extracts are evaporated in vacuo to 100 liters and the aqueous residue extracted three times with ligroin using 100 liters of ligroin each time. The ligroin, which contains fatty acids, resins and β-sitosterol removed from the residue is decanted and the residue, in ethanol solution, is diluted with 300 liters of water and extracted three times with dichloroethane using 100 liters each time.
The dichloroethane solution so obtained is evaporated to dryness in vacuo and 2.5 kg. of residue are obtained containing sericic acid and other free triterpenes (Fraction A).
The aqueous residue remaining after the extraction with dichloroethane is diluted with 500 liters of alcohol and 10 kg. of neutral lead acetate dissolved in 30 liters of alcohol are added. An abundant precipitate is formed and is left to stand overnight to sediment. The precipitate is centrifuged and discarded, and the water-alcohol phase is concentrated in vacuo to about 200 liters. The aqueous concentrate is extracted three times with a mixture of t-butanol-benzene in the ratio 3:1 (v/v) using 100 liters each time.
The organic phase is separated and washed with a 10% solution of sodium carbonate and then evaporated in vacuo to 20 liters. The butanolic concentrate so obtained is poured, with vigorous agitation, into 150 liters of diethyl ether. A precipitate comprising triterpenic glucosides is formed and is centrifuged and dried. 5 kg. of product are obtained, containing sericoside and other triterpenic glucosides (Fraction B).
1 kg. of Fraction A is dissolved in 5 liters of alcohol. The solution is decolorized with carbon, evaporated to 3 liters and left to stand overnight.
300 g. of crude sericic acid crystallize, which by successive recrystallization from dilute aqueous alcohol or acetic acid enables 200 g. of pure sericic acid having m.p. 278° - 82° C., [α]D 30 =+ 37.8 (c = 0.32 EtOH 95) to be obtained.
Additional quantities of sericic acid may be obtained by reuniting the mother liquors, which are then evaporated to dryness, to obtain a residue which is acetylated in acetone, using acetic anhydride. 80 g. of sericic acid triacetate are obtained which after recrystallization from glacial acetic acid melts at 183° C. and has [α]D =+4° (c = 2, EtOH 95) IR 3630 cm-1 1745 cm-1 and 1705 cm-1.
By saponification with alcoholic potash the sericic acid triacetate may be converted to sericic acid, in a state of purity such that its properties are entirely identical with that obtained by crystallization, as described above.
1 kg. of Fraction A is dissolved in 3 volumes of chloroform and chromatographed on a column containing 15 kg. of silica gel by eluting with a solvent mixture comprising 95:5 chloroform - ethanol. The fractions containing the individual pure components are collected and reunited and after crystallization from methanol, 250 g. of pure sericic acid having a m.p. 278° - 82° C. are obtained along with 20 g. of arjunic acid with m.p. 220 ° - 22° C.
1 kg. of Fraction B is dissolved in 6 liters of alcohol. The solution is decolorized with carbon and evaporated to 3 liters.
1 liter of hot water (at about 50° C.) is added and the liquid is left to stand overnight. 600 g. of crude sericoside crystallize which, after repeated crystallizations from dilute alcohol, furnish 250 g. of pure sericoside having the following characteristics: m.p. 206° - 208° C. and [α]D = + 5.4 (c = 2. pyridine).
1 kg. of fraction B is dissolved in 3 liters of an 8:2 (v/v) chloroform - ethanol mixture and chromatographed on 30 kg. of silica gel by eluting with the said solvent mixture. The fractions containing the individual pure products are reunited and concentrated to dryness. After crystallization from methanol there are obtained respectively 320 g. of sericoside having an m.p. of 206 - 208° C. and 210 g. of arjunetine.
10 g. of sericic acid are dissolved in 50 ml. of chloroform and treated with a solution of diazomethane in methylene chloride until complete reaction. The solution is concentrated in vacuo to dryness and the residue is crystallized from acetone.
10 g. of methyl sericate are dissolved in 50 ml. of anhydrous pyridine and 9.24 g. of benzoyl chloride are added. The reaction mixture is left to stand for one night, then poured into water. Tribenzoyl methyl sericate precipitates, and after crystallization from methanol has a melting point of 199° C.
10 g. of methyl triacetylsericate (prepared in an analogous manner to methyl tribenzoyl sericate) are dissolved in 500 ml. of anhydrous acetone and Jones's reagent is added until the reaction is complete.
The acetone solution is diluted with 1,500 ml. of water whereupon the product precipitates in amorphous form.
The product is collected by filtration, and recrystallized from aqeuous methanol. After drying, there are obtained 9 g. of triacetyl 19-keto methyl sericate having an m.p. of 200 and [α]D = + 33 (c = 0.5, EtOH).
10 g. of potassium sericate are dissolved in 50 ml. of dimethyl formamide and treated with 3 g. of diethylaminoethyl chloride. The reaction mixture is kept at 50° C. for 5 hours, then poured into 600 ml. of water. An abundant precipitate forms which is filtered, washed with water and recrystallized from aqueous isopropanol. The product melts at 105° - 8° C. and [α]D = +19.7° (c = 1, EtOH).
20 g. of methyl triacetylsericate are dissolved in 30 ml. of acetic anhydride and 1 ml. of concentrated perchloric acid is added. The reaction mixture is left to stand for 1 hour, then poured into 500 ml. of water.
There is precipitation of the product which, after filtration, is crystallized from ethanol.
The product melts at 223° C.
10 g. of methyl triacetyl sericate are dissolved in 50 ml. of 40% hydrobromic acid in acetic acid and left at room temperature for 30 hours. The reaction mixture is poured into water. The product is filtered and precipitates, and after drying it is crystallized from hexane. 6 g. of methyl tetracetyl 18α-sericate are obtained having an m.p. of 194° C. and [α]D =+12.6° (c = 0.4 in EtOH).
10 g. of sericic acid are dissolved in 50 ml. of anhydrous pyridine and 10 g of succinic anhydride are added.
The reaction mixture is reheated to 80° C. for 6 hours, then cooled and diluted with 800 ml. of chloroform.
The solution is counter-washed with 10% aqueous HCl until the pyridine is eliminated and then concentrated to dryness and dehydrated over Na2 SO4.
The residue is crystallized from glacial acetic acid and 11 g. of trihemisuccinyl sericic acid with m.p. of 105° - 108° C. and [α]D =+2.65° (c = 2, EtOH) is obtained.
By following the general procedures of the above Examples 2 to 8, but using appropriate alternative reactants other compounds of general formulae (II) or (III) may be prepared.
The following pharmaceutical data is given to illustrate the anti-inflammatory and cicatrizing properties of the compounds of the invention:
The anti-ulcer activity of sericic acid and sericoside was determined by administering the compounds to rats in which gastric ulcers were induced by Shay's method. The compounds were administered at the dose of 200 mg./kg. orally five times, at 42, 30, 25 and 6 hours before tying of the pylorus and immediately after the operation and as shown in Table 1 diminished the ulcer index by 48 and 45 per cent respectively, compared with the controls.
Table 1
__________________________________________________________________________
Percentage ulcer
Treatment Number of
Number of ulcers of each class.sup.(2)
Ulcer
variation in rel-
Number of
non-ulcerated
Substance
mg/kg
animals
I II III
IV V Index
ation to controls
stomachs (per
__________________________________________________________________________
cent)
Controls (gum 338 36 10 9 9 75 -- --
arabic 10%)
-- 13 (338)
(180)
(100)
(180)
(180)
Sericoside
200 13 369 15 1 0 3 39.5
-48 7.6
(369)
(75)
(10)
(0) (60)
Sericic acid
200 13 279 12 3 2 9 45 -45 --
(279)
(60)
(30)
(40)
(180)
__________________________________________________________________________
.sup.(1) Doses administered orally 5 times, 42, 30, 25 and 6 hours prior
to and immediately after the tying of the pylorus.
.sup.(2) In parentheses the product of the number of ulcers and the
numerical value attributed to the individual classes according to the
individual classes according to the evaluation criterion of T. O.
Keyrilainen and M. K. Paasonen (Acta Pharmacol. et. Toxicol. 13, 22, 1957
LD 50 in Mice
The LD50 in mice (as determined with intraperitoneal administration) for sericic acid and sericoside was as follows:
Sericic acid > 1,000 mg./kg.
Sericoside > 1,000 mg./kg.
The anti-inflammatory activities of sericoside and sericic acid were determined by measuring the extent to which the oedema caused by sub-plantar administration of carragenin to rats could be inhibited by prior oral and intraperitoneal administration of the substances.
The following results were obtained:
______________________________________
1. Oral Administration
Volume of
No. oedema
of in ml. Percentage
Treat- Doses ani- (Average inhibition
ment mg/kg.sup.(1)
mals ±S.D).sup.(2)
of the oedema
______________________________________
Controls
-- 10 0.30 ± 0.005
--
Sericoside
200 10 *0.22 ± 0.010
26
Sericic acid
200 10 *0.23 ± 0.009
23
______________________________________
*Significantly different (P < 0.05) from the average obtained with the
controls according to Student's "t" test.
.sup.(1) Doses administered orally for 3 days; on the third day the
administration was effected two hours before the subplantar injection of
carragenin.
.sup.(2) Maximum volume of the oedema measured 3 hours after the
subplantar injection of carragenin.
______________________________________
Volume of the
Doses No. oedema in ml.
Percentage
mg/kg of Average± S.D.)
inhibition of
Treatment
(1) animals (2) the oedema
______________________________________
Controls
-- 10 0.31 ± 0.010
--
Sericoside
100 10 0.10 ±0 0.008 (*)
67
Sericic Acid
100 10 0.11 ± 0.006 (*)
64
Controls
-- 10 0.31 ± 0.006
--
Sericoside
50 10 0.20 ± 0.005 (*)
35
Sericic acid
50 10 0.23 ± 0.008 (*)
25
______________________________________
(*) Significantly different (P<0.05) from the average obtained with the
controls according to Student's "t" test.
(1) Administration endoperitoneally 30 minutes prior to the injection of
carragenin.
(2) Maximum volume measured after 3 hours.
The cicatrizing activity of sericic acid and sericoside upon experimental wounds was determined on rats in accordance with the method of Morton & Malone (Arch. Int. Pharmacodyn. Ther. 196, 117, 1972).
The treatment of the wounds was effected with a 10% suspension of the active principles in water containing 2% carboxymethyl cellulose (CMC). The suspensions were used in doses of 0.1 ml. for each application, one treatment being effected per day.
From the data shown in the following Table 2 it can be seen that sericic acid and sericoside possess significant healing activity, compared with the controls, especially in the first days of treatment.
Table 2
__________________________________________________________________________
Cicatrizing activity upon experimental wounds in the rat
ml./ No.
rat/ of Percentage remargination. Average ± S.D.
Treatment
day
animals
Day I Day II
Day III
Day V Day VI
__________________________________________________________________________
Control
0.1
14 5.2 ± 1.1
12.8 ± 1.5
21.9 ± 3.1
39.63 ± 2.3
57.4 ± 2.1
(2% CMC)
Sericoside
0.1
14 13.9 ± 1.4*
23.7 ± 1.5*
36.8 ± 2.0*
53.30 ± 2.8*
68.2 ± 2.1*
Sericic acid
0.1
14 10.4 ± 1.3*
23.7 ± 2.3*
36.9 ± 2.7*
48.00 ± 1.5*
59.0 ± 2.2
__________________________________________________________________________
Note: Topical treatment effected on each of the days on which measurement
were taken (I, II, III, V, and VI).
*Significantly (P<0.05) different from the controls (2% CMC) based upon
Student's "t" test.
Ultra-violet ray erythema in guinea pigs was effected in accordance with the method of Winder et al. (Arch. Int. Pharmacodyn, 106, 261, 1968).
Sericoside and sericic acid were applied to the depilated skin of male albino guinea pigs (average weight 300 - 400 g) in the form of a 5% gel (using 200 mg. thereof) one hour prior to irradiation. The animals were depilated 18 hours prior to the irradiation and irradiated for 3 minutes with a 500 W. mercury vapor lamp at a distance of 18 cms.
The controls were treated with a similar gel which did not contain the active ingredients, sericoside or sericic acid.
The compounds under examination were able to reduce the erythema with a most pronounced activity at 8 hours, as shown by the results set forth in the following Table 3.
Table 3
__________________________________________________________________________
UV Erythema in the Guinea Pig
Number of Erythema Score**
Treatment
animals
2 h. 4 h. 6 h. 8 h. 22 h.
__________________________________________________________________________
Controls
10 1.15 ± 0.17
2.40 ± 0.30
2.55 ± 0.34
2.35 ± 0.18
1.05 ± 0.23
Sericoside
10 0.75 ± 0.17
1.70 ± 0.23
1.95 ± 0.22
1.30 ± 0.25*
0.70 ± 0.15
(34.8) (29.2) (23.5) (44.7) (33.3)
Sericic acid
10 0.95 ± 0.12
1.80 ± 0.21
2.05 ± 0.22
1.35 ± 0.18*
0.60 ± 0.10
(17.4) (25) (19.6) (42.5) (42.8)
__________________________________________________________________________
*Significantly (P<0.05) different from the controls, according to Duncan'
test (analysis of the variation).
**0 = no erythema; 0.5 = moderate reddening; 1 = marked reddening; 2 =
obvious erythema.
Note: The percentage inhibition compared with the controls is entered in
parentheses.
The following examples of pharmaceutical preparations were prepared by admixing the active ingredients with the excipients referred to and illustrate the manner in which the compounds of the invention may be brought into forms suitable for topical and oral administration.
______________________________________
1 % Gel for Topical Application
______________________________________
1) Sericoside 1 g.
Excipients (propylene glycol, Carbopol 934, ethyl
alcohol, triethanolamine, water,
100 g.
antifermentative) q.s.
2) Sericic acid 1 g.
Excipients (propylene glycol, Carbopol 934, ethyl
alcohol, triethanolamine, water,
100 g.
antifermentative) q.s.
______________________________________
______________________________________
1 % Ointment for Topical Application
______________________________________
Sericoside 1 g.
Excipients (glycerine, cetyl alcohol, saturated vegetable
triglycerides, lanoline oil, propylene glycol, water) q.s.
100 g.
______________________________________
______________________________________
Suspension for Oral Administration
______________________________________
Sericic acid 1 g.
Excipients (sodium alginate, corn starch, saccarose,
water, antifermentative) q.s.
100 g.
______________________________________
______________________________________ Powder for Topical Application ______________________________________ 1) Sericoside 2 g. Excipients (Microlan, corn starch, magnesium stearate, talc) q.s. 100 g. 2) Sericic acid 2 g. Excipients (Microlan, corn starch, magnesium stearate, talc) q.s. 100 g. ______________________________________
______________________________________ Ampoule for Injection ______________________________________ 1) Sericic acid 10 mg. Excipients (propylene glycol, ethyl alcohol, sterile pyrogen free water) q.s. 1 ml. 2) Sericoside 20 mg. Excipients (propylene glycol, ethyl alcohol, sterile pyrogen-free water) q.s. 2 ml. ______________________________________
______________________________________ Confections ______________________________________ 1) Sericoside 20 mg. Excipients (corn starch, lactose, talc, magnesium stearate, sodium alginate, sugar, gum arabic, magnesium carbonate) q.s. 250 mg. 2) Sericic acid 10 mg. Excipients (corn starch, lactose, talc, magnesium stearate, sodium alginate, sugar, gum arabic, magnesium carbonate) q.s. 200 mg. ______________________________________
______________________________________ Transparent gel ______________________________________ Sericic acid 10 g. Excipients (propylene glycol, triethanolamine, water, antifermentative) q.s. to 200 g. ______________________________________
______________________________________ Transparent gel ______________________________________ Sericoside 5 g. Excipients (propylene glycol, triethanolamine, water, antifermentative) q.s. to 200 g. ______________________________________
______________________________________
Tablets
______________________________________
Sericoside 100 mg.
Excipients (starch, lactose) q.s. to
500 mg.
______________________________________
Claims (46)
1. A derivative of sericic acid, having anti-inflammatory and cicatrizing properties, comprising a compound of the general formula ##STR4## in which the hydrogen atom attached to C18 is in the beta configuration, and in which R1, R2, R3 and R4 each represent a hydrogen atom or a substituted or unsubstituted aliphatic or aromatic mono- or polycarboxylic acid acyl radical, wherein when more than one of R1, R2, R3 and R4 are acyl radicals, they are the same acyl radical, and R5 represents a hydrogen atom or a substituted or unsubstituted aliphatic radical, and in which R1 to R5 are not all hydrogen.
2. A compound in accordance with claim 1 wherein one or more of R1, R2, R3 and R4 is an unsubstituted acyl radical selected from the group consisting of acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl, heptanoyl, hemi-maleyl, hemi-fumaryl, hemi-succinyl, benzoyl and phenylacetyl.
3. A compound in accordance with claim 2 wherein said acyl radical is hemi-maleyl, hemi-fumaryl, or hemi-succinyl in which the remaining carboxyl group is in the form of the free acid, an acid addition salt with a pharmaceutically acceptable cation or an ester with an aliphatic alcohol containing up to seven carbon atoms.
4. A compound in accordance with claim 1 wherein one or more of R1, R2, R3 and R4 is a substituted acyl radical selected from the group consisting of acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl, heptanoyl, hemi-maleyl, hemi-fumaryl, hemi-succinyl, benzoyl and phenylacetyl, wherein said substituents are selected from the group consisting of halogen, nitro, hydroxy, ether, keto and amino groups.
5. A compound in accordance with claim 4 wherein said acyl radical is benzoyl or phenylacetyl, substituted by one or more substituents selected from the group consisting of halogen, nitro, hydroxy, ether, keto and amino groups and aliphatic radicals containing up to six carbon atoms, said aliphatic radicals being unsubstituted or substituted by one or more of halogen, nitro, hydroxy, ether, keto and amino groups.
6. A compound as claimed in claim 1 in which each of R1, R2, R3 and R4 represents hydrogen.
7. A compound as claimed in claim 1 in which each of R1, R2, R3 and R4 represents acetyl.
8. A compound as claimed in claim 1 in which each of R1, R2, R3 and R4 represents benzoyl.
9. A compound as claimed in claim 1 in which each of R1, R2, R3 and R4 represents hemisuccinyl.
10. A compound as claimed in claim 1 in which R5 is an alkyl group.
11. A compound as claimed in claim 1 in which R5 is an aminoalkyl or a mono- or dialkylaminoalkyl group.
12. A compound as claimed in claim 11 in which each of R1, R2, R3 and R4 represent hydrogen.
13. A compound as claimed in claim 1 in which R5 is hydrogen.
14. A compound as claimed in claim 1 in which R5 is methyl.
15. A compound as claimed in claim 1 in which R5 is diethylaminoethyl.
16. A compound in accordance with claim 1 wherein R5 is --R'--NR"R'", wherein R' is a straight or branched chain alkylene radical containing up to seven carbon atoms and R" and R'", which may be the same or different, are hydrogen atoms or a straight or branched chain alkylene radical containing up to seven carbon atoms.
17. A compound in accordance with claim 16 wherein R1, R2, R3 and R4 are all hydrogen.
18. Methyl sericate as claimed in claim 1.
19. Tribenzoyl methyl sericate as claimed in claim 1.
20. Diethylaminoethyl sericate as claimed in claim 1.
21. Methyl tetracetyl sericate as claimed in claim 1.
22. 2,3,24-Trihemisuccinyl sericic acid as claimed in claim 1.
23. A pharmaceutically acceptable salt of a compound according to claim 1 capable of salt formation.
24. Sericic acid, having the general formula ##STR5## in pure form.
25. Sericic acid in accordance with claim 24 in crystalline form.
26. Methyl tetracetyl 18α-sericate.
27. A pharmaceutical composition consisting essentially of an anti-inflammatory amount of an active principle of the general formula ##STR6## in which the hydrogen atom attached to C18 is in the beta configuration, and in which R1, R2, R3 and R4 each represents a hydrogen atom or a substituted or unsubstituted aliphatic or aromatic mono-or polycarboxylic acid acyl radical, wherein when more than one of R1, R2, R3 and R4 are acyl radicals, they are the same acyl radical, and R5 represents a hydrogen atom or a substituted or an unsubstituted aliphatic radical, and a pharmaceutically acceptable excipient.
28. A composition in accordance with claim 27, wherein the active principle is sericic acid.
29. A composition in accordance with claim 27, wherein the active principle is a dialkylaminoalkyl sericate.
30. A method of ellicting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a compound as defined in claim 1.
31. A method of elliciting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a compound as defined in claim 24.
32. A method of elliciting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a composition in accordance with claim 27.
33. A method of elliciting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a compound as defined in claim 28.
34. A method of elliciting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a compound as defined in claim 29.
35. A pharmaceutical composition consisting essentially of a cicatrizing amount of an active principle of the general formula ##STR7## in which the hydrogen atom attached to C18 is in the beta configuration, and in which R1, R2, R3 and R4 each represents a hydrogen atom or a substituted or unsubstituted aliphatic or aromatic mono/or polycarboxylic acid acyl radical, wherein when more than one of R1, R2, R3 and R4 are acyl radicals, they are the same acyl radical, and R5 represents a hydrogen atom or a substituted or an unsubstituted aliphatic radical, and a pharmaceutically acceptable excipient.
36. A composition in accordance with claim 35, wherein the active principle is sericic acid.
37. A composition in accordance with claim 35, wherein the active principle is a dialkylaminoalkyl sericate.
38. A method of elliciting a cicatrizing effect in an animal, which comprises administering a cicatrizing amount of a compound as defined in claim 1.
39. A method of elliciting a cicatrizing effect in an animal, which comprises administering a cicatrizing amount of a compound as defined in claim 24.
40. A method of elliciting a cicatrizing effect in an animal, which comprises administering a cicatrizing amount of a composition in accordance with claim 35.
41. A method of elliciting a cicatrizing effect in an animal, which comprising administering a cicatrizing amount of a compound as defined in claim 36.
42. A method of elliciting a cicatrizing effect in an animal, which comprises administering a cicatrizing amount of a compound as defined in claim 37.
43. A pharmaceutical composition consisting essentially of an anti-inflammatory amount of the compound of claim 26 and a pharmaceutically acceptable excipient.
44. A method of elliciting an anti-inflammatory effect in an animal, which comprises administering an anti-inflammatory amount of a compound as defined in claim 26.
45. A pharmaceutical composition consisting essentially of a cicatrizing amount of the compound of claim 26 and a pharmaceutically acceptable excipient.
46. A method of elliciting a cicatrizing effect in an animal, which comprises administering a cicatrizing amount of a compound as defined in claim 26.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4042/74A GB1493926A (en) | 1974-01-29 | 1974-01-29 | Pentacyclic triterpenes obtainable from terminalia sericea and their derivatives |
| GB4042/74 | 1974-01-29 | ||
| US54158875A | 1975-01-16 | 1975-01-16 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US54158875A Continuation | 1974-01-29 | 1975-01-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US4103025A true US4103025A (en) | 1978-07-25 |
Family
ID=26238793
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US05/778,457 Expired - Lifetime US4147777A (en) | 1974-01-29 | 1977-03-17 | Sericosides and method of use |
| US05/778,458 Expired - Lifetime US4103025A (en) | 1974-01-29 | 1977-03-17 | Novel terpenes |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US05/778,457 Expired - Lifetime US4147777A (en) | 1974-01-29 | 1977-03-17 | Sericosides and method of use |
Country Status (1)
| Country | Link |
|---|---|
| US (2) | US4147777A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10121092A1 (en) * | 2001-04-26 | 2002-10-31 | Beiersdorf Ag | Cosmetic or dermatological formulations containing sericoside and / or plant extracts containing the same |
| DE10121090A1 (en) * | 2001-04-26 | 2002-10-31 | Beiersdorf Ag | New cosmetic or dermatological compositions comprise a synergistic combination of sericoside and tetrahydrocurcumin or its derivatives, useful for treating e.g. skin inflammation and aging symptoms |
| US20070122506A1 (en) * | 2005-11-25 | 2007-05-31 | Khanuja Suman P S | Process for the isolation of oleane compounds isolated from the bark of arjun tree Terminalia arjuna (Roxb.) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1195849B (en) * | 1986-07-01 | 1988-10-27 | Sigma Tau Ind Farmaceuti | TRITERPENIC SOAPS WITH ANTI-INFLAMMATORY, MUCOLITIC AND ANTI-EDEMIGEN ACTIVITY, PROCEDURE FOR THEIR OBTAINING AND PHARMACEUTICAL COMPOSITIONS THAT INCLUDE THEM |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1530409A (en) * | 1963-08-28 | 1968-06-28 | New derivatives of asiaticoside and their preparation process | |
| DE1617667B1 (en) * | 1966-09-08 | 1970-09-03 | Nattermann A & Cie | Process for the production of a sennosid-rich active ingredient concentrate from sennessee pods |
| US3723410A (en) * | 1970-12-01 | 1973-03-27 | Amazon Natural Drug Co | Method of producing stevioside |
-
1977
- 1977-03-17 US US05/778,457 patent/US4147777A/en not_active Expired - Lifetime
- 1977-03-17 US US05/778,458 patent/US4103025A/en not_active Expired - Lifetime
Non-Patent Citations (6)
| Title |
|---|
| Bombardelli et al., cited in CA, 82, 98192e (1975). * |
| Row et al., C.A, 63, p. 18178 (1965). * |
| Row et al., Indian Journal of Chemistry, vol. 6, pp. 716-721 (1970). * |
| Row et al., Tetrahedron Letters, No. 27, pp. 12-16 (1960). * |
| row et al., Tetrahedron Letters, No. 4, 129-134 (1962). * |
| Row et al., Tetrahedron, vol. 18, pp. 827-838 (1962). * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10121092A1 (en) * | 2001-04-26 | 2002-10-31 | Beiersdorf Ag | Cosmetic or dermatological formulations containing sericoside and / or plant extracts containing the same |
| DE10121090A1 (en) * | 2001-04-26 | 2002-10-31 | Beiersdorf Ag | New cosmetic or dermatological compositions comprise a synergistic combination of sericoside and tetrahydrocurcumin or its derivatives, useful for treating e.g. skin inflammation and aging symptoms |
| US20070122506A1 (en) * | 2005-11-25 | 2007-05-31 | Khanuja Suman P S | Process for the isolation of oleane compounds isolated from the bark of arjun tree Terminalia arjuna (Roxb.) |
| US7435433B2 (en) * | 2005-11-25 | 2008-10-14 | Council Of Scientific & Industrial Research | Process for the isolation of oleane compounds isolated from the bark of arjun tree terminalia arjuna (Roxb.) |
Also Published As
| Publication number | Publication date |
|---|---|
| US4147777A (en) | 1979-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0210785B1 (en) | Process for obtaining proanthocyanidine a2, pharmaceutical compositions and their therapeutic use | |
| US5629351A (en) | Boswellic acid compositions and preparation thereof | |
| US5652265A (en) | Production of rhein and rhein derivatives | |
| US5273747A (en) | Commiphora mukul extracts and therapeutical applications thereof | |
| SK9498A3 (en) | Use of calendula glycosides for the treatment of psoriasis | |
| CH645386A5 (en) | SAPONIN, PROCESS FOR ITS PREPARATION AND MEDICAMENTS CONTAINING THIS SAPONIN. | |
| CA2072283C (en) | Process for the preparation of diacetylrhein | |
| US4879376A (en) | Triterpene saponins having anti-inflammatory, mucolytic and antiedemic activities, process for the preparation thereof and pharmaceutical compositions containing them | |
| DE2821403C2 (en) | Decahydronaphthalene-1-spiro-2'-dihydrobenzofurans, processes for their preparation and pharmaceutical compositions containing these compounds | |
| Quick | THE PREPARATION OF BORNEOL GLYCURONIC ACID AND GLYCURONIC ACID. | |
| US4206222A (en) | Method for preparing a substance having properties against collagen diseases and products obtained | |
| Finnegan et al. | Chemical Examination of a Toxic Extract of Indigofera Endecaphylla: The Endecaphyllins | |
| US4103025A (en) | Novel terpenes | |
| CA1061777A (en) | Terpenes | |
| NZ232445A (en) | 2,3,23-trihydroxy-urs-12-ene derivatives as agents to treat some brain-related disorders; preparatory processes and use | |
| JPS6212791A (en) | Astragali radix saponin, isolation and use thereof | |
| US3364113A (en) | Senna preparations and methods of making and using them | |
| US4061773A (en) | Glycyrrhetinic acid derivatives | |
| Garg et al. | Blighia sapida I. Constituents of the fresh fruit | |
| US4618605A (en) | Process for the production of β-elemonic acid and pharmaceutical preparations containing said acid | |
| DE69206321T2 (en) | Ursane derivatives, their preparation and the pharmaceutical composition containing them. | |
| US4162255A (en) | Process for extracting aristolochic acids | |
| JP2640239B2 (en) | Protostar-13 (17) ene-3,16-dione compound | |
| US3579505A (en) | Alisol compounds | |
| JPH09202729A (en) | Arthritis treating agent composed of diacetylrhein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AS | Assignment |
Owner name: IDB HOLDING S.P.A., VIA RIPAMONTI 99, MILANO Free format text: CHANGE OF NAME;ASSIGNOR:INVERNI DELLA BEFFA S.P.A.;REEL/FRAME:005471/0361 Effective date: 19901015 |